RESISTANCE protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1467 |
| Acc36I | ACCTGC | 1 cut(s) | 1535 |
| AccB1I | GGYRCC | 1 cut(s) | 1718 |
| AccII | CGCG | 1 cut(s) | 1716 |
| AccIII | TCCGGA | 3 cut(s) | 481, 1264, 1321 |
| AciI | CCGC | 9 cut(s) | 35, 873, 1043, 1138, 1343, 1433, 1515, 1716, 2059 |
| AclWI | GGATC | 4 cut(s) | 189, 1121, 1275, 1691 |
| AcsI | RAATTY | 8 cut(s) | 83, 212, 506, 609, 715, 1053, 1171, 1317 |
| AcuI | CTGAAG | 1 cut(s) | 177 |
| AcvI | CACGTG | 1 cut(s) | 1763 |
| AfaI | GTAC | 5 cut(s) | 46, 93, 391, 1113, 2035 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 655 |
| AjiI | CACGTC | 1 cut(s) | 1077 |
| AjnI | CCWGG | 3 cut(s) | 1842, 1917, 2003 |
| Alw21I | GWGCWC | 3 cut(s) | 845, 1038, 1578 |
| Alw26I | GTCTC | 2 cut(s) | 902, 1501 |
| AlwI | GGATC | 4 cut(s) | 189, 1121, 1275, 1691 |
| Ama87I | CYCGRG | 1 cut(s) | 1306 |
| Aor13HI | TCCGGA | 3 cut(s) | 481, 1264, 1321 |
| AoxI | GGCC | 3 cut(s) | 160, 982, 1727 |
| ApeKI | GCWGC | 5 cut(s) | 294, 870, 1045, 1071, 1611 |
| ApoI | RAATTY | 8 cut(s) | 83, 212, 506, 609, 715, 1053, 1171, 1317 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 330 |
| AspLEI | GCGC | 2 cut(s) | 1421, 2022 |
| AspS9I | GGNCC | 2 cut(s) | 1858, 1933 |
| AsuC2I | CCSGG | 4 cut(s) | 96, 686, 1307, 1308 |
| AsuHPI | GGTGA | 1 cut(s) | 1493 |
| AvaI | CYCGRG | 1 cut(s) | 1306 |
| AvaII | GGWCC | 2 cut(s) | 1858, 1933 |
| BanI | GGYRCC | 1 cut(s) | 1718 |
| BanII | GRGCYC | 2 cut(s) | 1038, 1525 |
| BbrPI | CACGTG | 1 cut(s) | 1763 |
| BbsI | GAAGAC | 3 cut(s) | 143, 1890, 1965 |
| Bbv12I | GWGCWC | 3 cut(s) | 845, 1038, 1578 |
| BbvI | GCAGC | 5 cut(s) | 306, 857, 1032, 1058, 1623 |
| BccI | CCATC | 3 cut(s) | 214, 272, 887 |
| BceAI | ACGGC | 1 cut(s) | 1678 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 271, 305 |
| BciT130I | CCWGG | 3 cut(s) | 1844, 1919, 2005 |
| BciVI | GTATCC | 1 cut(s) | 150 |
| BclI | TGATCA | 2 cut(s) | 1864, 1939 |
| BcnI | CCSGG | 4 cut(s) | 96, 686, 1307, 1308 |
| BcoDI | GTCTC | 2 cut(s) | 902, 1501 |
| BfaI | CTAG | 7 cut(s) | 129, 809, 932, 1049, 1638, 1985, 1992 |
| BfmI | CTRYAG | 1 cut(s) | 250 |
| BfoI | RGCGCY | 1 cut(s) | 2023 |
| BfuAI | ACCTGC | 1 cut(s) | 1535 |
| BfuI | GTATCC | 1 cut(s) | 150 |
| BglII | AGATCT | 3 cut(s) | 310, 346, 550 |
| BisI | GCNGC | 6 cut(s) | 295, 871, 1046, 1072, 1612, 1717 |
| BlsI | GCNGC | 6 cut(s) | 296, 872, 1047, 1073, 1613, 1718 |
| Bme1390I | CCNGG | 7 cut(s) | 96, 686, 1307, 1308, 1844, 1919, 2005 |
| Bme18I | GGWCC | 2 cut(s) | 1858, 1933 |
| BmeT110I | CYCGRG | 1 cut(s) | 1306 |
| BmgBI | CACGTC | 1 cut(s) | 1077 |
| BmgT120I | GGNCC | 2 cut(s) | 1858, 1933 |
| BmiI | GGNNCC | 6 cut(s) | 342, 1720, 1859, 1860, 1934, 1935 |
| BmrFI | CCNGG | 7 cut(s) | 96, 686, 1307, 1308, 1844, 1919, 2005 |
| BmrI | ACTGGG | 1 cut(s) | 187 |
| BmsI | GCATC | 8 cut(s) | 475, 480, 730, 765, 998, 1198, 1244, 1531 |
| BmuI | ACTGGG | 1 cut(s) | 187 |
| BpiI | GAAGAC | 3 cut(s) | 143, 1890, 1965 |
| BpmI | CTGGAG | 2 cut(s) | 292, 1359 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1584 |
| BpuEI | CTTGAG | 3 cut(s) | 387, 980, 1612 |
| BpuMI | CCSGG | 4 cut(s) | 96, 686, 1307, 1308 |
| BsaAI | YACGTR | 2 cut(s) | 224, 1763 |
| BsaI | GGTCTC | 1 cut(s) | 902 |
| BsaJI | CCNNGG | 8 cut(s) | 1306, 1842, 1843, 1890, 1917, 1918, 1965, 2004 |
| BsaWI | WCCGGW | 4 cut(s) | 481, 1264, 1321, 1776 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1020, 1050 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 655 |
| Bse1I | ACTGG | 1 cut(s) | 182 |
| Bse3DI | GCAATG | 5 cut(s) | 706, 914, 1079, 1425, 1516 |
| BseAI | TCCGGA | 3 cut(s) | 481, 1264, 1321 |
| BseBI | CCWGG | 3 cut(s) | 1844, 1919, 2005 |
| BseDI | CCNNGG | 8 cut(s) | 1306, 1842, 1843, 1890, 1917, 1918, 1965, 2004 |
| BseGI | GGATG | 3 cut(s) | 490, 1210, 1819 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 655 |
| BseMI | GCAATG | 5 cut(s) | 706, 914, 1079, 1425, 1516 |
| BseMII | CTCAG | 5 cut(s) | 29, 135, 368, 394, 843 |
| BseNI | ACTGG | 1 cut(s) | 182 |
| BseXI | GCAGC | 5 cut(s) | 306, 857, 1032, 1058, 1623 |
| BsgI | GTGCAG | 2 cut(s) | 547, 1057 |
| Bsh1236I | CGCG | 1 cut(s) | 1716 |
| Bsh1285I | CGRYCG | 1 cut(s) | 592 |
| BshFI | GGCC | 3 cut(s) | 162, 984, 1729 |
| BshNI | GGYRCC | 1 cut(s) | 1718 |
| BsiEI | CGRYCG | 1 cut(s) | 592 |
| BsiHKAI | GWGCWC | 3 cut(s) | 845, 1038, 1578 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1306 |
| BsiSI | CCGG | 7 cut(s) | 96, 482, 686, 1265, 1307, 1322, 1777 |
| BsiWI | CGTACG | 1 cut(s) | 44 |
| BslFI | GGGAC | 3 cut(s) | 1871, 1946, 2021 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 655 |
| BsmAI | GTCTC | 2 cut(s) | 902, 1501 |
| BsmFI | GGGAC | 3 cut(s) | 1871, 1946, 2021 |
| BsnI | GGCC | 3 cut(s) | 162, 984, 1729 |
| Bso31I | GGTCTC | 1 cut(s) | 902 |
| BsoBI | CYCGRG | 1 cut(s) | 1306 |
| Bsp1286I | GDGCHC | 4 cut(s) | 845, 1038, 1525, 1578 |
| Bsp13I | TCCGGA | 3 cut(s) | 481, 1264, 1321 |
| Bsp1407I | TGTACA | 2 cut(s) | 389, 1111 |
| Bsp19I | CCATGG | 2 cut(s) | 1890, 1965 |
| BspACI | CCGC | 9 cut(s) | 35, 873, 1043, 1138, 1343, 1433, 1515, 1716, 2059 |
| BspANI | GGCC | 3 cut(s) | 162, 984, 1729 |
| BspCNI | CTCAG | 5 cut(s) | 30, 136, 367, 393, 842 |
| BspEI | TCCGGA | 3 cut(s) | 481, 1264, 1321 |
| BspFNI | CGCG | 1 cut(s) | 1716 |
| BspHI | TCATGA | 1 cut(s) | 454 |
| BspLI | GGNNCC | 6 cut(s) | 342, 1720, 1859, 1860, 1934, 1935 |
| BspMI | ACCTGC | 1 cut(s) | 1535 |
| BspPI | GGATC | 4 cut(s) | 189, 1121, 1275, 1691 |
| BspT107I | GGYRCC | 1 cut(s) | 1718 |
| BspTNI | GGTCTC | 1 cut(s) | 902 |
| BsrDI | GCAATG | 5 cut(s) | 706, 914, 1079, 1425, 1516 |
| BsrGI | TGTACA | 2 cut(s) | 389, 1111 |
| BsrI | ACTGG | 1 cut(s) | 182 |
| BssECI | CCNNGG | 8 cut(s) | 1306, 1842, 1843, 1890, 1917, 1918, 1965, 2004 |
| BssT1I | CCWWGG | 2 cut(s) | 1890, 1965 |
| Bst2UI | CCWGG | 3 cut(s) | 1844, 1919, 2005 |
| Bst4CI | ACNGT | 5 cut(s) | 75, 923, 1111, 1783, 2038 |
| BstAUI | TGTACA | 2 cut(s) | 389, 1111 |
| BstBAI | YACGTR | 2 cut(s) | 224, 1763 |
| BstC8I | GCNNGC | 3 cut(s) | 120, 1727, 2024 |
| BstDEI | CTNAG | 9 cut(s) | 38, 144, 164, 314, 354, 380, 511, 829, 1584 |
| BstDSI | CCRYGG | 2 cut(s) | 1890, 1965 |
| BstF5I | GGATG | 3 cut(s) | 490, 1210, 1819 |
| BstFNI | CGCG | 1 cut(s) | 1716 |
| BstH2I | RGCGCY | 1 cut(s) | 2023 |
| BstHHI | GCGC | 2 cut(s) | 1421, 2022 |
| BstMAI | GTCTC | 2 cut(s) | 902, 1501 |
| BstMCI | CGRYCG | 1 cut(s) | 592 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 244, 1042, 1418, 1427, 1722 |
| BstNI | CCWGG | 3 cut(s) | 1844, 1919, 2005 |
| BstNSI | RCATGY | 1 cut(s) | 1680 |
| BstSCI | CCNGG | 7 cut(s) | 94, 684, 1305, 1306, 1842, 1917, 2003 |
| BstSFI | CTRYAG | 1 cut(s) | 250 |
| BstUI | CGCG | 1 cut(s) | 1716 |
| BstV1I | GCAGC | 5 cut(s) | 306, 857, 1032, 1058, 1623 |
| BstV2I | GAAGAC | 3 cut(s) | 143, 1890, 1965 |
| BstX2I | RGATCY | 5 cut(s) | 310, 346, 550, 1126, 1683 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1709 |
| BstYI | RGATCY | 5 cut(s) | 310, 346, 550, 1126, 1683 |
| BsuI | GTATCC | 1 cut(s) | 150 |
| BsuRI | GGCC | 3 cut(s) | 162, 984, 1729 |
| BtgI | CCRYGG | 2 cut(s) | 1890, 1965 |
| BtgZI | GCGATG | 1 cut(s) | 503 |
| BtrI | CACGTC | 1 cut(s) | 1077 |
| BtsCI | GGATG | 3 cut(s) | 490, 1210, 1819 |
| BtsIMutI | CAGTG | 2 cut(s) | 175, 731 |
| BveI | ACCTGC | 1 cut(s) | 1535 |
| Cac8I | GCNNGC | 3 cut(s) | 120, 1727, 2024 |
| CciI | TCATGA | 1 cut(s) | 454 |
| CfoI | GCGC | 2 cut(s) | 1421, 2022 |
| Cfr13I | GGNCC | 2 cut(s) | 1858, 1933 |
| Cfr9I | CCCGGG | 1 cut(s) | 1306 |
| CseI | GACGC | 4 cut(s) | 1285, 1515, 1632, 1722 |
| Csp6I | GTAC | 5 cut(s) | 45, 92, 390, 1112, 2034 |
| CviQI | GTAC | 5 cut(s) | 45, 92, 390, 1112, 2034 |
| DdeI | CTNAG | 9 cut(s) | 38, 144, 164, 314, 354, 380, 511, 829, 1584 |
| DraI | TTTAAA | 2 cut(s) | 196, 421 |
| Ecl136II | GAGCTC | 1 cut(s) | 1036 |
| Eco130I | CCWWGG | 2 cut(s) | 1890, 1965 |
| Eco147I | AGGCCT | 1 cut(s) | 162 |
| Eco24I | GRGCYC | 2 cut(s) | 1038, 1525 |
| Eco31I | GGTCTC | 1 cut(s) | 902 |
| Eco32I | GATATC | 1 cut(s) | 170 |
| Eco47I | GGWCC | 2 cut(s) | 1858, 1933 |
| Eco53kI | GAGCTC | 1 cut(s) | 1036 |
| Eco57I | CTGAAG | 1 cut(s) | 177 |
| Eco72I | CACGTG | 1 cut(s) | 1763 |
| Eco88I | CYCGRG | 1 cut(s) | 1306 |
| EcoICRI | GAGCTC | 1 cut(s) | 1036 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1858, 1933 |
| EcoRI | GAATTC | 1 cut(s) | 506 |
| EcoRII | CCWGG | 3 cut(s) | 1842, 1917, 2003 |
| EcoRV | GATATC | 1 cut(s) | 170 |
| EcoT14I | CCWWGG | 2 cut(s) | 1890, 1965 |
| EcoT38I | GRGCYC | 2 cut(s) | 1038, 1525 |
| ErhI | CCWWGG | 2 cut(s) | 1890, 1965 |
| FaqI | GGGAC | 3 cut(s) | 1871, 1946, 2021 |
| FauI | CCCGC | 2 cut(s) | 866, 1508 |
| FauNDI | CATATG | 1 cut(s) | 243 |
| FbaI | TGATCA | 2 cut(s) | 1864, 1939 |
| Fnu4HI | GCNGC | 6 cut(s) | 295, 871, 1046, 1072, 1612, 1717 |
| FokI | GGATG | 3 cut(s) | 497, 1217, 1826 |
| FriOI | GRGCYC | 2 cut(s) | 1038, 1525 |
| Fsp4HI | GCNGC | 6 cut(s) | 295, 871, 1046, 1072, 1612, 1717 |
| FspBI | CTAG | 7 cut(s) | 129, 809, 932, 1049, 1638, 1985, 1992 |
| GlaI | GCGC | 2 cut(s) | 1420, 2021 |
| GluI | GCNGC | 6 cut(s) | 295, 871, 1046, 1072, 1612, 1717 |
| GsuI | CTGGAG | 2 cut(s) | 292, 1359 |
| HaeII | RGCGCY | 1 cut(s) | 2023 |
| HaeIII | GGCC | 3 cut(s) | 162, 984, 1729 |
| HapII | CCGG | 7 cut(s) | 96, 482, 686, 1265, 1307, 1322, 1777 |
| HgaI | GACGC | 4 cut(s) | 1285, 1515, 1632, 1722 |
| HhaI | GCGC | 2 cut(s) | 1421, 2022 |
| Hin6I | GCGC | 2 cut(s) | 1419, 2020 |
| HinP1I | GCGC | 2 cut(s) | 1419, 2020 |
| HindIII | AAGCTT | 4 cut(s) | 394, 434, 638, 1016 |
| HpaII | CCGG | 7 cut(s) | 96, 482, 686, 1265, 1307, 1322, 1777 |
| HphI | GGTGA | 1 cut(s) | 1493 |
| Hpy166II | GTNNAC | 2 cut(s) | 390, 1744 |
| Hpy8I | GTNNAC | 2 cut(s) | 390, 1744 |
| HpyAV | CCTTC | 7 cut(s) | 61, 152, 420, 624, 835, 995, 1666 |
| HpyCH4III | ACNGT | 5 cut(s) | 75, 923, 1111, 1783, 2038 |
| HpyCH4IV | ACGT | 4 cut(s) | 223, 1076, 1261, 1762 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 244, 1042, 1418, 1427, 1722 |
| HpyF3I | CTNAG | 9 cut(s) | 38, 144, 164, 314, 354, 380, 511, 829, 1584 |
| HpySE526I | ACGT | 4 cut(s) | 223, 1076, 1261, 1762 |
| HspAI | GCGC | 2 cut(s) | 1419, 2020 |
| KflI | GGGWCCC | 2 cut(s) | 1858, 1933 |
| Kpn2I | TCCGGA | 3 cut(s) | 481, 1264, 1321 |
| Ksp22I | TGATCA | 2 cut(s) | 1864, 1939 |
| LmnI | GCTCC | 3 cut(s) | 13, 1041, 1191 |
| Lsp1109I | GCAGC | 5 cut(s) | 306, 857, 1032, 1058, 1623 |
| LweI | GCATC | 8 cut(s) | 475, 480, 730, 765, 998, 1198, 1244, 1531 |
| MaeI | CTAG | 7 cut(s) | 129, 809, 932, 1049, 1638, 1985, 1992 |
| MaeII | ACGT | 4 cut(s) | 223, 1076, 1261, 1762 |
| MaeIII | GTNAC | 4 cut(s) | 224, 690, 1633, 1758 |
| MfeI | CAATTG | 1 cut(s) | 363 |
| MflI | RGATCY | 5 cut(s) | 310, 346, 550, 1126, 1683 |
| MhlI | GDGCHC | 4 cut(s) | 845, 1038, 1525, 1578 |
| MlyI | GAGTC | 3 cut(s) | 49, 367, 394 |
| MmeI | TCCRAC | 2 cut(s) | 572, 782 |
| MroI | TCCGGA | 3 cut(s) | 481, 1264, 1321 |
| MroXI | GAANNNNTTC | 1 cut(s) | 330 |
| MseI | TTAA | 5 cut(s) | 195, 299, 420, 713, 767 |
| MslI | CAYNNNNRTG | 2 cut(s) | 59, 1119 |
| MspA1I | CMGCKG | 2 cut(s) | 37, 1045 |
| MspI | CCGG | 7 cut(s) | 96, 482, 686, 1265, 1307, 1322, 1777 |
| MspR9I | CCNGG | 7 cut(s) | 96, 686, 1307, 1308, 1844, 1919, 2005 |
| MunI | CAATTG | 1 cut(s) | 363 |
| MvaI | CCWGG | 3 cut(s) | 1844, 1919, 2005 |
| MvnI | CGCG | 1 cut(s) | 1716 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 244, 1042, 1418, 1427, 1722 |
| NciI | CCSGG | 4 cut(s) | 96, 686, 1307, 1308 |
| NcoI | CCATGG | 2 cut(s) | 1890, 1965 |
| NdeI | CATATG | 1 cut(s) | 243 |
| NlaIV | GGNNCC | 6 cut(s) | 342, 1720, 1859, 1860, 1934, 1935 |
| NmeAIII | GCCGAG | 1 cut(s) | 270 |
| NmuCI | GTSAC | 2 cut(s) | 1633, 1758 |
| NspI | RCATGY | 1 cut(s) | 1680 |
| PagI | TCATGA | 1 cut(s) | 454 |
| PasI | CCCWGGG | 2 cut(s) | 1843, 1918 |
| PceI | AGGCCT | 1 cut(s) | 162 |
| PdmI | GAANNNNTTC | 1 cut(s) | 330 |
| PfeI | GAWTC | 7 cut(s) | 286, 448, 652, 928, 1286, 1447, 1706 |
| Pfl23II | CGTACG | 1 cut(s) | 44 |
| PfoI | TCCNGGA | 1 cut(s) | 684 |
| PkrI | GCNGC | 6 cut(s) | 296, 872, 1047, 1073, 1613, 1718 |
| PleI | GAGTC | 3 cut(s) | 48, 366, 394 |
| PmaCI | CACGTG | 1 cut(s) | 1763 |
| PmlI | CACGTG | 1 cut(s) | 1763 |
| PpsI | GAGTC | 3 cut(s) | 48, 366, 394 |
| Ppu21I | YACGTR | 2 cut(s) | 224, 1763 |
| PpuMI | RGGWCCY | 2 cut(s) | 1858, 1933 |
| PsiI | TTATAA | 1 cut(s) | 1467 |
| Psp124BI | GAGCTC | 1 cut(s) | 1038 |
| Psp5II | RGGWCCY | 2 cut(s) | 1858, 1933 |
| Psp6I | CCWGG | 3 cut(s) | 1842, 1917, 2003 |
| PspCI | CACGTG | 1 cut(s) | 1763 |
| PspGI | CCWGG | 3 cut(s) | 1842, 1917, 2003 |
| PspLI | CGTACG | 1 cut(s) | 44 |
| PspN4I | GGNNCC | 6 cut(s) | 342, 1720, 1859, 1860, 1934, 1935 |
| PspPI | GGNCC | 2 cut(s) | 1858, 1933 |
| PspPPI | RGGWCCY | 2 cut(s) | 1858, 1933 |
| PsuI | RGATCY | 5 cut(s) | 310, 346, 550, 1126, 1683 |
| RsaI | GTAC | 5 cut(s) | 46, 93, 391, 1113, 2035 |
| RsaNI | GTAC | 5 cut(s) | 45, 92, 390, 1112, 2034 |
| RseI | CAYNNNNRTG | 2 cut(s) | 59, 1119 |
| SacI | GAGCTC | 1 cut(s) | 1038 |
| SaqAI | TTAA | 5 cut(s) | 195, 299, 420, 713, 767 |
| SatI | GCNGC | 6 cut(s) | 295, 871, 1046, 1072, 1612, 1717 |
| Sau96I | GGNCC | 2 cut(s) | 1858, 1933 |
| SchI | GAGTC | 3 cut(s) | 49, 367, 394 |
| ScrFI | CCNGG | 7 cut(s) | 96, 686, 1307, 1308, 1844, 1919, 2005 |
| SduI | GDGCHC | 4 cut(s) | 845, 1038, 1525, 1578 |
| SfaNI | GCATC | 8 cut(s) | 475, 480, 730, 765, 998, 1198, 1244, 1531 |
| SfcI | CTRYAG | 1 cut(s) | 250 |
| SinI | GGWCC | 2 cut(s) | 1858, 1933 |
| SmaI | CCCGGG | 1 cut(s) | 1308 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 59, 1119 |
| SmlI | CTYRAG | 3 cut(s) | 402, 995, 1627 |
| SmoI | CTYRAG | 3 cut(s) | 402, 995, 1627 |
| SseBI | AGGCCT | 1 cut(s) | 162 |
| SsiI | CCGC | 9 cut(s) | 35, 873, 1043, 1138, 1343, 1433, 1515, 1716, 2059 |
| SspI | AATATT | 1 cut(s) | 571 |
| SspMI | CTAG | 7 cut(s) | 129, 809, 932, 1049, 1638, 1985, 1992 |
| SstI | GAGCTC | 1 cut(s) | 1038 |
| StuI | AGGCCT | 1 cut(s) | 162 |
| StyD4I | CCNGG | 7 cut(s) | 94, 684, 1305, 1306, 1842, 1917, 2003 |
| StyI | CCWWGG | 2 cut(s) | 1890, 1965 |
| TaaI | ACNGT | 5 cut(s) | 75, 923, 1111, 1783, 2038 |
| TaiI | ACGT | 4 cut(s) | 226, 1079, 1264, 1765 |
| TaqI | TCGA | 3 cut(s) | 429, 786, 1241 |
| TaqII | GACCGA | 1 cut(s) | 578 |
| TatI | WGTACW | 3 cut(s) | 389, 1111, 2033 |
| TauI | GCSGC | 1 cut(s) | 1719 |
| TfiI | GAWTC | 7 cut(s) | 286, 448, 652, 928, 1286, 1447, 1706 |
| Tru1I | TTAA | 5 cut(s) | 195, 299, 420, 713, 767 |
| Tru9I | TTAA | 5 cut(s) | 195, 299, 420, 713, 767 |
| TscAI | CASTG | 2 cut(s) | 182, 731 |
| TseFI | GTSAC | 2 cut(s) | 1633, 1758 |
| TseI | GCWGC | 5 cut(s) | 294, 870, 1045, 1071, 1611 |
| Tsp45I | GTSAC | 2 cut(s) | 1633, 1758 |
| TspGWI | ACGGA | 2 cut(s) | 1228, 1521 |
| TspMI | CCCGGG | 1 cut(s) | 1306 |
| TspRI | CASTG | 2 cut(s) | 182, 731 |
| VpaK11BI | GGWCC | 2 cut(s) | 1858, 1933 |
| XapI | RAATTY | 8 cut(s) | 83, 212, 506, 609, 715, 1053, 1171, 1317 |
| XceI | RCATGY | 1 cut(s) | 1680 |
| XmaI | CCCGGG | 1 cut(s) | 1306 |
| XmnI | GAANNNNTTC | 1 cut(s) | 330 |
| XspI | CTAG | 7 cut(s) | 129, 809, 932, 1049, 1638, 1985, 1992 |