RESISTANCE protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AbsI | CCTCGAGG | 1 cut(s) | 261 |
| Acc16I | TGCGCA | 1 cut(s) | 1120 |
| AccII | CGCG | 1 cut(s) | 1092 |
| AciI | CCGC | 3 cut(s) | 373, 1132, 1242 |
| AclWI | GGATC | 1 cut(s) | 1119 |
| AcsI | RAATTY | 2 cut(s) | 25, 322 |
| AcuI | CTGAAG | 3 cut(s) | 93, 150, 371 |
| AfaI | GTAC | 1 cut(s) | 56 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 951 |
| AflII | CTTAAG | 1 cut(s) | 200 |
| AflIII | ACRYGT | 1 cut(s) | 208 |
| AgsI | TTSAA | 7 cut(s) | 116, 175, 322, 676, 998, 1055, 1103 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 232 |
| AjnI | CCWGG | 1 cut(s) | 908 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 990, 1022 |
| Alw21I | GWGCWC | 2 cut(s) | 648, 1274 |
| Alw26I | GTCTC | 5 cut(s) | 62, 272, 790, 976, 1078 |
| AlwI | GGATC | 1 cut(s) | 1119 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 71, 535, 733 |
| Ama87I | CYCGRG | 2 cut(s) | 261, 269 |
| AoxI | GGCC | 1 cut(s) | 45 |
| ApeKI | GCWGC | 8 cut(s) | 417, 526, 724, 778, 841, 876, 1244, 1247 |
| ApoI | RAATTY | 2 cut(s) | 25, 322 |
| AspLEI | GCGC | 1 cut(s) | 1121 |
| AspS9I | GGNCC | 1 cut(s) | 226 |
| AsuC2I | CCSGG | 2 cut(s) | 13, 224 |
| AsuHPI | GGTGA | 2 cut(s) | 245, 967 |
| AvaI | CYCGRG | 2 cut(s) | 261, 269 |
| AvaII | GGWCC | 1 cut(s) | 226 |
| BanII | GRGCYC | 1 cut(s) | 648 |
| BbsI | GAAGAC | 1 cut(s) | 269 |
| Bbv12I | GWGCWC | 2 cut(s) | 648, 1274 |
| BbvCI | CCTCAGC | 1 cut(s) | 791 |
| BbvI | GCAGC | 8 cut(s) | 404, 513, 711, 765, 828, 863, 1231, 1234 |
| BccI | CCATC | 4 cut(s) | 302, 340, 866, 1212 |
| BciT130I | CCWGG | 1 cut(s) | 910 |
| BcnI | CCSGG | 2 cut(s) | 13, 224 |
| BcoDI | GTCTC | 5 cut(s) | 62, 272, 790, 976, 1078 |
| BfaI | CTAG | 1 cut(s) | 887 |
| BfmI | CTRYAG | 2 cut(s) | 527, 725 |
| BfrI | CTTAAG | 1 cut(s) | 200 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 787 |
| BglII | AGATCT | 1 cut(s) | 991 |
| BisI | GCNGC | 9 cut(s) | 418, 527, 725, 779, 842, 877, 1242, 1245, 1248 |
| BlpI | GCTNAGC | 1 cut(s) | 461 |
| BlsI | GCNGC | 9 cut(s) | 419, 528, 726, 780, 843, 878, 1243, 1246, 1249 |
| Bme1390I | CCNGG | 3 cut(s) | 13, 224, 910 |
| Bme18I | GGWCC | 1 cut(s) | 226 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 232 |
| BmeT110I | CYCGRG | 2 cut(s) | 261, 269 |
| BmgT120I | GGNCC | 1 cut(s) | 226 |
| BmiI | GGNNCC | 3 cut(s) | 227, 228, 1038 |
| BmrFI | CCNGG | 3 cut(s) | 13, 224, 910 |
| BmsI | GCATC | 1 cut(s) | 171 |
| BpiI | GAAGAC | 1 cut(s) | 269 |
| BpmI | CTGGAG | 2 cut(s) | 126, 931 |
| Bpu10I | CCTNAGC | 1 cut(s) | 791 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 461 |
| BpuEI | CTTGAG | 6 cut(s) | 267, 449, 496, 562, 760, 972 |
| BpuMI | CCSGG | 2 cut(s) | 13, 224 |
| BsaI | GGTCTC | 1 cut(s) | 272 |
| BsaJI | CCNNGG | 2 cut(s) | 845, 908 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 951 |
| Bse1I | ACTGG | 3 cut(s) | 109, 474, 1096 |
| Bse3DI | GCAATG | 1 cut(s) | 43 |
| BseBI | CCWGG | 1 cut(s) | 910 |
| BseDI | CCNNGG | 2 cut(s) | 845, 908 |
| BseGI | GGATG | 2 cut(s) | 313, 1223 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 951 |
| BseMI | GCAATG | 1 cut(s) | 43 |
| BseMII | CTCAG | 2 cut(s) | 452, 782 |
| BseNI | ACTGG | 3 cut(s) | 109, 474, 1096 |
| BseRI | GAGGAG | 1 cut(s) | 808 |
| BseXI | GCAGC | 8 cut(s) | 404, 513, 711, 765, 828, 863, 1231, 1234 |
| Bsh1236I | CGCG | 1 cut(s) | 1092 |
| BshFI | GGCC | 1 cut(s) | 47 |
| BsiHKAI | GWGCWC | 2 cut(s) | 648, 1274 |
| BsiHKCI | CYCGRG | 2 cut(s) | 261, 269 |
| BsiSI | CCGG | 2 cut(s) | 12, 223 |
| BslFI | GGGAC | 2 cut(s) | 239, 649 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 951 |
| BsmAI | GTCTC | 5 cut(s) | 62, 272, 790, 976, 1078 |
| BsmBI | CGTCTC | 1 cut(s) | 1078 |
| BsmFI | GGGAC | 2 cut(s) | 239, 649 |
| BsmI | GAATGC | 1 cut(s) | 122 |
| BsnI | GGCC | 1 cut(s) | 47 |
| Bso31I | GGTCTC | 1 cut(s) | 272 |
| BsoBI | CYCGRG | 2 cut(s) | 261, 269 |
| Bsp1286I | GDGCHC | 2 cut(s) | 648, 1274 |
| Bsp143I | GATC | 4 cut(s) | 148, 669, 991, 1111 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 461 |
| Bsp19I | CCATGG | 1 cut(s) | 845 |
| BspACI | CCGC | 3 cut(s) | 373, 1132, 1242 |
| BspANI | GGCC | 1 cut(s) | 47 |
| BspCNI | CTCAG | 2 cut(s) | 453, 783 |
| BspFNI | CGCG | 1 cut(s) | 1092 |
| BspHI | TCATGA | 1 cut(s) | 126 |
| BspLI | GGNNCC | 3 cut(s) | 227, 228, 1038 |
| BspMAI | CTGCAG | 2 cut(s) | 531, 729 |
| BspPI | GGATC | 1 cut(s) | 1119 |
| BspTI | CTTAAG | 1 cut(s) | 200 |
| BspTNI | GGTCTC | 1 cut(s) | 272 |
| BsrDI | GCAATG | 1 cut(s) | 43 |
| BsrI | ACTGG | 3 cut(s) | 109, 474, 1096 |
| BssECI | CCNNGG | 2 cut(s) | 845, 908 |
| BssMI | GATC | 4 cut(s) | 148, 669, 991, 1111 |
| BssT1I | CCWWGG | 1 cut(s) | 845 |
| Bst2UI | CCWGG | 1 cut(s) | 910 |
| Bst4CI | ACNGT | 3 cut(s) | 801, 949, 1281 |
| BstAFI | CTTAAG | 1 cut(s) | 200 |
| BstDEI | CTNAG | 6 cut(s) | 121, 191, 290, 461, 791, 1080 |
| BstDSI | CCRYGG | 1 cut(s) | 845 |
| BstF5I | GGATG | 2 cut(s) | 313, 1223 |
| BstFNI | CGCG | 1 cut(s) | 1092 |
| BstHHI | GCGC | 1 cut(s) | 1121 |
| BstKTI | GATC | 4 cut(s) | 151, 672, 994, 1114 |
| BstMAI | GTCTC | 5 cut(s) | 62, 272, 790, 976, 1078 |
| BstMBI | GATC | 4 cut(s) | 148, 669, 991, 1111 |
| BstNI | CCWGG | 1 cut(s) | 910 |
| BstNSI | RCATGY | 2 cut(s) | 212, 883 |
| BstSCI | CCNGG | 3 cut(s) | 11, 222, 908 |
| BstSFI | CTRYAG | 2 cut(s) | 527, 725 |
| BstUI | CGCG | 1 cut(s) | 1092 |
| BstV1I | GCAGC | 8 cut(s) | 404, 513, 711, 765, 828, 863, 1231, 1234 |
| BstV2I | GAAGAC | 1 cut(s) | 269 |
| BstX2I | RGATCY | 1 cut(s) | 991 |
| BstYI | RGATCY | 1 cut(s) | 991 |
| BsuRI | GGCC | 1 cut(s) | 47 |
| BtgI | CCRYGG | 1 cut(s) | 845 |
| BtsCI | GGATG | 2 cut(s) | 313, 1223 |
| BtsI | GCAGTG | 1 cut(s) | 308 |
| BtsIMutI | CAGTG | 5 cut(s) | 308, 533, 731, 945, 1121 |
| CaiI | CAGNNNCTG | 3 cut(s) | 71, 535, 733 |
| CciI | TCATGA | 1 cut(s) | 126 |
| CfoI | GCGC | 1 cut(s) | 1121 |
| Cfr13I | GGNCC | 1 cut(s) | 226 |
| CseI | GACGC | 2 cut(s) | 229, 1098 |
| Csp6I | GTAC | 1 cut(s) | 55 |
| CviAII | CATG | 7 cut(s) | 127, 133, 209, 237, 409, 846, 880 |
| CviQI | GTAC | 1 cut(s) | 55 |
| DdeI | CTNAG | 6 cut(s) | 121, 191, 290, 461, 791, 1080 |
| DpnI | GATC | 4 cut(s) | 150, 671, 993, 1113 |
| DpnII | GATC | 4 cut(s) | 148, 669, 991, 1111 |
| DraI | TTTAAA | 1 cut(s) | 864 |
| DriI | GACNNNNNGTC | 1 cut(s) | 232 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 232 |
| EciI | GGCGGA | 1 cut(s) | 1121 |
| Ecl136II | GAGCTC | 1 cut(s) | 646 |
| Eco130I | CCWWGG | 1 cut(s) | 845 |
| Eco24I | GRGCYC | 1 cut(s) | 648 |
| Eco31I | GGTCTC | 1 cut(s) | 272 |
| Eco32I | GATATC | 1 cut(s) | 1066 |
| Eco47I | GGWCC | 1 cut(s) | 226 |
| Eco53kI | GAGCTC | 1 cut(s) | 646 |
| Eco57I | CTGAAG | 3 cut(s) | 93, 150, 371 |
| Eco88I | CYCGRG | 2 cut(s) | 261, 269 |
| EcoICRI | GAGCTC | 1 cut(s) | 646 |
| EcoO109I | RGGNCCY | 1 cut(s) | 226 |
| EcoRI | GAATTC | 1 cut(s) | 322 |
| EcoRII | CCWGG | 1 cut(s) | 908 |
| EcoRV | GATATC | 1 cut(s) | 1066 |
| EcoT14I | CCWWGG | 1 cut(s) | 845 |
| EcoT22I | ATGCAT | 1 cut(s) | 186 |
| EcoT38I | GRGCYC | 1 cut(s) | 648 |
| ErhI | CCWWGG | 1 cut(s) | 845 |
| Esp3I | CGTCTC | 1 cut(s) | 1078 |
| FaeI | CATG | 7 cut(s) | 130, 136, 212, 240, 412, 849, 883 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 240, 272, 916, 948 |
| FaqI | GGGAC | 2 cut(s) | 239, 649 |
| FatI | CATG | 7 cut(s) | 126, 132, 208, 236, 408, 845, 879 |
| Fnu4HI | GCNGC | 9 cut(s) | 418, 527, 725, 779, 842, 877, 1242, 1245, 1248 |
| FokI | GGATG | 2 cut(s) | 320, 1230 |
| FriOI | GRGCYC | 1 cut(s) | 648 |
| Fsp4HI | GCNGC | 9 cut(s) | 418, 527, 725, 779, 842, 877, 1242, 1245, 1248 |
| FspBI | CTAG | 1 cut(s) | 887 |
| FspI | TGCGCA | 1 cut(s) | 1120 |
| GlaI | GCGC | 1 cut(s) | 1120 |
| GluI | GCNGC | 9 cut(s) | 418, 527, 725, 779, 842, 877, 1242, 1245, 1248 |
| GsuI | CTGGAG | 2 cut(s) | 126, 931 |
| HaeIII | GGCC | 1 cut(s) | 47 |
| HapII | CCGG | 2 cut(s) | 12, 223 |
| HgaI | GACGC | 2 cut(s) | 229, 1098 |
| HhaI | GCGC | 1 cut(s) | 1121 |
| Hin1II | CATG | 7 cut(s) | 130, 136, 212, 240, 412, 849, 883 |
| Hin6I | GCGC | 1 cut(s) | 1119 |
| HinP1I | GCGC | 1 cut(s) | 1119 |
| HincII | GTYRAC | 1 cut(s) | 244 |
| HindII | GTYRAC | 1 cut(s) | 244 |
| HinfI | GANTC | 8 cut(s) | 5, 490, 556, 622, 688, 754, 820, 969 |
| HpaII | CCGG | 2 cut(s) | 12, 223 |
| HphI | GGTGA | 2 cut(s) | 245, 967 |
| Hpy166II | GTNNAC | 4 cut(s) | 244, 253, 945, 1151 |
| Hpy188I | TCNGA | 1 cut(s) | 148 |
| Hpy188III | TCNNGA | 8 cut(s) | 127, 152, 269, 347, 428, 673, 989, 995 |
| Hpy8I | GTNNAC | 4 cut(s) | 244, 253, 945, 1151 |
| Hpy99I | CGWCG | 1 cut(s) | 1092 |
| HpyCH4III | ACNGT | 3 cut(s) | 801, 949, 1281 |
| HpyF3I | CTNAG | 6 cut(s) | 121, 191, 290, 461, 791, 1080 |
| Hsp92II | CATG | 7 cut(s) | 130, 136, 212, 240, 412, 849, 883 |
| HspAI | GCGC | 1 cut(s) | 1119 |
| KflI | GGGWCCC | 1 cut(s) | 226 |
| Kzo9I | GATC | 4 cut(s) | 148, 669, 991, 1111 |
| LmnI | GCTCC | 4 cut(s) | 107, 651, 912, 1134 |
| Lsp1109I | GCAGC | 8 cut(s) | 404, 513, 711, 765, 828, 863, 1231, 1234 |
| LweI | GCATC | 1 cut(s) | 171 |
| MaeI | CTAG | 1 cut(s) | 887 |
| MaeIII | GTNAC | 6 cut(s) | 61, 204, 218, 882, 935, 1281 |
| MalI | GATC | 4 cut(s) | 150, 671, 993, 1113 |
| MboI | GATC | 4 cut(s) | 148, 669, 991, 1111 |
| MboII | GAAGA | 3 cut(s) | 269, 1034, 1073 |
| MfeI | CAATTG | 4 cut(s) | 111, 613, 679, 1251 |
| MflI | RGATCY | 1 cut(s) | 991 |
| MhlI | GDGCHC | 2 cut(s) | 648, 1274 |
| MluCI | AATT | 8 cut(s) | 25, 111, 294, 322, 599, 613, 679, 1251 |
| MlyI | GAGTC | 6 cut(s) | 499, 565, 631, 697, 829, 978 |
| MnlI | CCTC | 9 cut(s) | 257, 270, 291, 431, 786, 885, 945, 1004, 1168 |
| Mph1103I | ATGCAT | 1 cut(s) | 186 |
| MseI | TTAA | 4 cut(s) | 201, 213, 863, 1212 |
| MslI | CAYNNNNRTG | 1 cut(s) | 131 |
| MspA1I | CMGCKG | 1 cut(s) | 1244 |
| MspCI | CTTAAG | 1 cut(s) | 200 |
| MspI | CCGG | 2 cut(s) | 12, 223 |
| MspR9I | CCNGG | 3 cut(s) | 13, 224, 910 |
| MunI | CAATTG | 4 cut(s) | 111, 613, 679, 1251 |
| Mva1269I | GAATGC | 1 cut(s) | 122 |
| MvaI | CCWGG | 1 cut(s) | 910 |
| MvnI | CGCG | 1 cut(s) | 1092 |
| NciI | CCSGG | 2 cut(s) | 13, 224 |
| NcoI | CCATGG | 1 cut(s) | 845 |
| NdeII | GATC | 4 cut(s) | 148, 669, 991, 1111 |
| NlaIII | CATG | 7 cut(s) | 130, 136, 212, 240, 412, 849, 883 |
| NlaIV | GGNNCC | 3 cut(s) | 227, 228, 1038 |
| NmuCI | GTSAC | 2 cut(s) | 882, 935 |
| NsbI | TGCGCA | 1 cut(s) | 1120 |
| NsiI | ATGCAT | 1 cut(s) | 186 |
| NspI | RCATGY | 2 cut(s) | 212, 883 |
| PaeR7I | CTCGAG | 2 cut(s) | 261, 269 |
| PagI | TCATGA | 1 cut(s) | 126 |
| PciI | ACATGT | 1 cut(s) | 208 |
| PctI | GAATGC | 1 cut(s) | 122 |
| PfeI | GAWTC | 2 cut(s) | 5, 754 |
| PkrI | GCNGC | 9 cut(s) | 419, 528, 726, 780, 843, 878, 1243, 1246, 1249 |
| PleI | GAGTC | 6 cut(s) | 498, 564, 630, 696, 828, 977 |
| PpsI | GAGTC | 6 cut(s) | 498, 564, 630, 696, 828, 977 |
| PpuMI | RGGWCCY | 1 cut(s) | 226 |
| PscI | ACATGT | 1 cut(s) | 208 |
| Psp124BI | GAGCTC | 1 cut(s) | 648 |
| Psp5II | RGGWCCY | 1 cut(s) | 226 |
| Psp6I | CCWGG | 1 cut(s) | 908 |
| PspGI | CCWGG | 1 cut(s) | 908 |
| PspN4I | GGNNCC | 3 cut(s) | 227, 228, 1038 |
| PspPI | GGNCC | 1 cut(s) | 226 |
| PspPPI | RGGWCCY | 1 cut(s) | 226 |
| PspXI | VCTCGAGB | 1 cut(s) | 261 |
| PstI | CTGCAG | 2 cut(s) | 531, 729 |
| PstNI | CAGNNNCTG | 3 cut(s) | 71, 535, 733 |
| PsuI | RGATCY | 1 cut(s) | 991 |
| RsaI | GTAC | 1 cut(s) | 56 |
| RsaNI | GTAC | 1 cut(s) | 55 |
| RseI | CAYNNNNRTG | 1 cut(s) | 131 |
| SacI | GAGCTC | 1 cut(s) | 648 |
| SaqAI | TTAA | 4 cut(s) | 201, 213, 863, 1212 |
| SatI | GCNGC | 9 cut(s) | 418, 527, 725, 779, 842, 877, 1242, 1245, 1248 |
| Sau3AI | GATC | 4 cut(s) | 148, 669, 991, 1111 |
| Sau96I | GGNCC | 1 cut(s) | 226 |
| SchI | GAGTC | 6 cut(s) | 499, 565, 631, 697, 829, 978 |
| ScrFI | CCNGG | 3 cut(s) | 13, 224, 910 |
| SduI | GDGCHC | 2 cut(s) | 648, 1274 |
| SfaNI | GCATC | 1 cut(s) | 171 |
| SfcI | CTRYAG | 2 cut(s) | 527, 725 |
| Sfr274I | CTCGAG | 2 cut(s) | 261, 269 |
| SinI | GGWCC | 1 cut(s) | 226 |
| SlaI | CTCGAG | 2 cut(s) | 261, 269 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 131 |
| SmlI | CTYRAG | 9 cut(s) | 200, 261, 269, 282, 428, 475, 541, 739, 987 |
| SmoI | CTYRAG | 9 cut(s) | 200, 261, 269, 282, 428, 475, 541, 739, 987 |
| Sse9I | AATT | 8 cut(s) | 25, 111, 294, 322, 599, 613, 679, 1251 |
| SsiI | CCGC | 3 cut(s) | 373, 1132, 1242 |
| SspMI | CTAG | 1 cut(s) | 887 |
| SstI | GAGCTC | 1 cut(s) | 648 |
| StyD4I | CCNGG | 3 cut(s) | 11, 222, 908 |
| StyI | CCWWGG | 1 cut(s) | 845 |
| TaaI | ACNGT | 3 cut(s) | 801, 949, 1281 |
| TaqI | TCGA | 6 cut(s) | 262, 270, 444, 576, 642, 774 |
| TasI | AATT | 8 cut(s) | 25, 111, 294, 322, 599, 613, 679, 1251 |
| TatI | WGTACW | 1 cut(s) | 54 |
| TauI | GCSGC | 1 cut(s) | 1244 |
| TfiI | GAWTC | 2 cut(s) | 5, 754 |
| Tru1I | TTAA | 4 cut(s) | 201, 213, 863, 1212 |
| Tru9I | TTAA | 4 cut(s) | 201, 213, 863, 1212 |
| TscAI | CASTG | 5 cut(s) | 308, 540, 738, 952, 1121 |
| TseFI | GTSAC | 2 cut(s) | 882, 935 |
| TseI | GCWGC | 8 cut(s) | 417, 526, 724, 778, 841, 876, 1244, 1247 |
| Tsp45I | GTSAC | 2 cut(s) | 882, 935 |
| TspDTI | ATGAA | 4 cut(s) | 143, 425, 1035, 1184 |
| TspGWI | ACGGA | 1 cut(s) | 611 |
| TspRI | CASTG | 5 cut(s) | 308, 540, 738, 952, 1121 |
| Vha464I | CTTAAG | 1 cut(s) | 200 |
| VpaK11BI | GGWCC | 1 cut(s) | 226 |
| XapI | RAATTY | 2 cut(s) | 25, 322 |
| XceI | RCATGY | 2 cut(s) | 212, 883 |
| XhoI | CTCGAG | 2 cut(s) | 261, 269 |
| XspI | CTAG | 1 cut(s) | 887 |
| Zsp2I | ATGCAT | 1 cut(s) | 186 |