No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 669 |
| AccBSI | CCGCTC | 4 cut(s) | 80, 399, 465, 540 |
| AccII | CGCG | 1 cut(s) | 14 |
| AciI | CCGC | 8 cut(s) | 14, 63, 80, 120, 207, 399, 465, 540 |
| AclWI | GGATC | 3 cut(s) | 29, 931, 944 |
| AcsI | RAATTY | 1 cut(s) | 181 |
| AcuI | CTGAAG | 5 cut(s) | 269, 401, 467, 533, 749 |
| AcvI | CACGTG | 1 cut(s) | 74 |
| AfaI | GTAC | 4 cut(s) | 320, 392, 458, 659 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 854, 855, 947, 975 |
| AgsI | TTSAA | 5 cut(s) | 181, 913, 1022, 1127, 1156 |
| AjiI | CACGTC | 1 cut(s) | 810 |
| AjnI | CCWGG | 1 cut(s) | 594 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 135, 167, 915, 947, 1014, 1046 |
| Alw21I | GWGCWC | 1 cut(s) | 35 |
| Alw26I | GTCTC | 4 cut(s) | 812, 829, 1000, 1102 |
| AlwI | GGATC | 3 cut(s) | 29, 931, 944 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 727 |
| AoxI | GGCC | 2 cut(s) | 94, 897 |
| ApeKI | GCWGC | 4 cut(s) | 204, 718, 769, 804 |
| ApoI | RAATTY | 1 cut(s) | 181 |
| AsuHPI | GGTGA | 1 cut(s) | 991 |
| BamHI | GGATCC | 1 cut(s) | 936 |
| BanII | GRGCYC | 1 cut(s) | 35 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 374, 406, 440, 472 |
| BbrPI | CACGTG | 1 cut(s) | 74 |
| Bbv12I | GWGCWC | 1 cut(s) | 35 |
| BbvI | GCAGC | 4 cut(s) | 191, 705, 756, 791 |
| BceAI | ACGGC | 1 cut(s) | 127 |
| BciT130I | CCWGG | 1 cut(s) | 596 |
| BcoDI | GTCTC | 4 cut(s) | 812, 829, 1000, 1102 |
| BfaI | CTAG | 2 cut(s) | 782, 815 |
| BfmI | CTRYAG | 2 cut(s) | 53, 719 |
| BfuAI | ACCTGC | 1 cut(s) | 669 |
| BisI | GCNGC | 5 cut(s) | 205, 208, 719, 770, 805 |
| BlsI | GCNGC | 5 cut(s) | 206, 209, 720, 771, 806 |
| BmcAI | AGTACT | 1 cut(s) | 320 |
| Bme1390I | CCNGG | 1 cut(s) | 596 |
| BmgBI | CACGTC | 1 cut(s) | 810 |
| BmiI | GGNNCC | 2 cut(s) | 938, 1062 |
| BmrFI | CCNGG | 1 cut(s) | 596 |
| BmsI | GCATC | 2 cut(s) | 784, 899 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 809, 841 |
| BpmI | CTGGAG | 4 cut(s) | 19, 278, 335, 674 |
| BpuEI | CTTGAG | 8 cut(s) | 236, 349, 415, 481, 622, 641, 688, 811 |
| Bsa29I | ATCGAT | 1 cut(s) | 24 |
| BsaAI | YACGTR | 1 cut(s) | 74 |
| BsaJI | CCNNGG | 2 cut(s) | 773, 894 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 854, 855, 947, 975 |
| Bse1I | ACTGG | 2 cut(s) | 36, 261 |
| BseBI | CCWGG | 1 cut(s) | 596 |
| BseCI | ATCGAT | 1 cut(s) | 24 |
| BseDI | CCNNGG | 2 cut(s) | 773, 894 |
| BseGI | GGATG | 2 cut(s) | 88, 890 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 854, 855, 947, 975 |
| BseMII | CTCAG | 1 cut(s) | 1144 |
| BseNI | ACTGG | 2 cut(s) | 36, 261 |
| BseXI | GCAGC | 4 cut(s) | 191, 705, 756, 791 |
| BsgI | GTGCAG | 3 cut(s) | 647, 790, 828 |
| Bsh1236I | CGCG | 1 cut(s) | 14 |
| BshFI | GGCC | 2 cut(s) | 96, 899 |
| BshVI | ATCGAT | 1 cut(s) | 24 |
| BsiHKAI | GWGCWC | 1 cut(s) | 35 |
| BslFI | GGGAC | 2 cut(s) | 267, 1130 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 854, 855, 947, 975 |
| BsmAI | GTCTC | 4 cut(s) | 812, 829, 1000, 1102 |
| BsmBI | CGTCTC | 1 cut(s) | 1102 |
| BsmFI | GGGAC | 2 cut(s) | 267, 1130 |
| BsnI | GGCC | 2 cut(s) | 96, 899 |
| Bsp1286I | GDGCHC | 1 cut(s) | 35 |
| Bsp143I | GATC | 2 cut(s) | 21, 936 |
| Bsp19I | CCATGG | 1 cut(s) | 773 |
| BspACI | CCGC | 8 cut(s) | 14, 63, 80, 120, 207, 399, 465, 540 |
| BspANI | GGCC | 2 cut(s) | 96, 899 |
| BspCNI | CTCAG | 1 cut(s) | 1143 |
| BspDI | ATCGAT | 1 cut(s) | 24 |
| BspFNI | CGCG | 1 cut(s) | 14 |
| BspLI | GGNNCC | 2 cut(s) | 938, 1062 |
| BspMAI | CTGCAG | 1 cut(s) | 723 |
| BspMI | ACCTGC | 1 cut(s) | 669 |
| BspPI | GGATC | 3 cut(s) | 29, 931, 944 |
| BsrBI | CCGCTC | 4 cut(s) | 80, 399, 465, 540 |
| BsrI | ACTGG | 2 cut(s) | 36, 261 |
| BssECI | CCNNGG | 2 cut(s) | 773, 894 |
| BssMI | GATC | 2 cut(s) | 21, 936 |
| BssT1I | CCWWGG | 1 cut(s) | 773 |
| Bst2UI | CCWGG | 1 cut(s) | 596 |
| Bst4CI | ACNGT | 3 cut(s) | 47, 823, 973 |
| BstBAI | YACGTR | 1 cut(s) | 74 |
| BstC8I | GCNNGC | 2 cut(s) | 98, 1150 |
| BstDEI | CTNAG | 2 cut(s) | 1104, 1130 |
| BstDSI | CCRYGG | 1 cut(s) | 773 |
| BstF5I | GGATG | 2 cut(s) | 88, 890 |
| BstFNI | CGCG | 1 cut(s) | 14 |
| BstKTI | GATC | 2 cut(s) | 24, 939 |
| BstMAI | GTCTC | 4 cut(s) | 812, 829, 1000, 1102 |
| BstMBI | GATC | 2 cut(s) | 21, 936 |
| BstNI | CCWGG | 1 cut(s) | 596 |
| BstSCI | CCNGG | 1 cut(s) | 594 |
| BstSFI | CTRYAG | 2 cut(s) | 53, 719 |
| BstUI | CGCG | 1 cut(s) | 14 |
| BstV1I | GCAGC | 4 cut(s) | 191, 705, 756, 791 |
| BstX2I | RGATCY | 1 cut(s) | 936 |
| BstYI | RGATCY | 1 cut(s) | 936 |
| Bsu15I | ATCGAT | 1 cut(s) | 24 |
| BsuRI | GGCC | 2 cut(s) | 96, 899 |
| BsuTUI | ATCGAT | 1 cut(s) | 24 |
| BtgI | CCRYGG | 1 cut(s) | 773 |
| BtrI | CACGTC | 1 cut(s) | 810 |
| BtsCI | GGATG | 2 cut(s) | 88, 890 |
| BtsIMutI | CAGTG | 3 cut(s) | 43, 969, 1138 |
| BveI | ACCTGC | 1 cut(s) | 669 |
| Cac8I | GCNNGC | 2 cut(s) | 98, 1150 |
| CaiI | CAGNNNCTG | 1 cut(s) | 727 |
| ClaI | ATCGAT | 1 cut(s) | 24 |
| Csp6I | GTAC | 4 cut(s) | 319, 391, 457, 658 |
| CviAII | CATG | 3 cut(s) | 200, 774, 1213 |
| CviQI | GTAC | 4 cut(s) | 319, 391, 457, 658 |
| DdeI | CTNAG | 2 cut(s) | 1104, 1130 |
| DpnI | GATC | 2 cut(s) | 23, 938 |
| DpnII | GATC | 2 cut(s) | 21, 936 |
| Ecl136II | GAGCTC | 1 cut(s) | 33 |
| Eco130I | CCWWGG | 1 cut(s) | 773 |
| Eco147I | AGGCCT | 1 cut(s) | 899 |
| Eco24I | GRGCYC | 1 cut(s) | 35 |
| Eco32I | GATATC | 2 cut(s) | 136, 1090 |
| Eco53kI | GAGCTC | 1 cut(s) | 33 |
| Eco57I | CTGAAG | 5 cut(s) | 269, 401, 467, 533, 749 |
| Eco72I | CACGTG | 1 cut(s) | 74 |
| EcoICRI | GAGCTC | 1 cut(s) | 33 |
| EcoRI | GAATTC | 1 cut(s) | 181 |
| EcoRII | CCWGG | 1 cut(s) | 594 |
| EcoRV | GATATC | 2 cut(s) | 136, 1090 |
| EcoT14I | CCWWGG | 1 cut(s) | 773 |
| EcoT38I | GRGCYC | 1 cut(s) | 35 |
| ErhI | CCWWGG | 1 cut(s) | 773 |
| Esp3I | CGTCTC | 1 cut(s) | 1102 |
| FaeI | CATG | 3 cut(s) | 203, 777, 1216 |
| FaiI | YATR | 8 cut(s) | 201, 407, 473, 775, 1008, 1058, 1167, 1214 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 84, 116, 135, 167 |
| FaqI | GGGAC | 2 cut(s) | 267, 1130 |
| FatI | CATG | 3 cut(s) | 199, 773, 1212 |
| FauI | CCCGC | 2 cut(s) | 7, 113 |
| Fnu4HI | GCNGC | 5 cut(s) | 205, 208, 719, 770, 805 |
| FokI | GGATG | 2 cut(s) | 95, 877 |
| FriOI | GRGCYC | 1 cut(s) | 35 |
| Fsp4HI | GCNGC | 5 cut(s) | 205, 208, 719, 770, 805 |
| FspBI | CTAG | 2 cut(s) | 782, 815 |
| GluI | GCNGC | 5 cut(s) | 205, 208, 719, 770, 805 |
| GsuI | CTGGAG | 4 cut(s) | 19, 278, 335, 674 |
| HaeIII | GGCC | 2 cut(s) | 96, 899 |
| Hin1II | CATG | 3 cut(s) | 203, 777, 1216 |
| HincII | GTYRAC | 1 cut(s) | 961 |
| HindII | GTYRAC | 1 cut(s) | 961 |
| HindIII | AAGCTT | 1 cut(s) | 1150 |
| HphI | GGTGA | 1 cut(s) | 991 |
| Hpy166II | GTNNAC | 1 cut(s) | 961 |
| Hpy188I | TCNGA | 4 cut(s) | 167, 729, 895, 936 |
| Hpy188III | TCNNGA | 3 cut(s) | 158, 215, 872 |
| Hpy8I | GTNNAC | 1 cut(s) | 961 |
| Hpy99I | CGWCG | 2 cut(s) | 134, 1116 |
| HpyCH4III | ACNGT | 3 cut(s) | 47, 823, 973 |
| HpyCH4IV | ACGT | 2 cut(s) | 73, 809 |
| HpyF3I | CTNAG | 2 cut(s) | 1104, 1130 |
| HpySE526I | ACGT | 2 cut(s) | 73, 809 |
| Hsp92II | CATG | 3 cut(s) | 203, 777, 1216 |
| Kzo9I | GATC | 2 cut(s) | 21, 936 |
| LmnI | GCTCC | 3 cut(s) | 38, 77, 192 |
| Lsp1109I | GCAGC | 4 cut(s) | 191, 705, 756, 791 |
| LweI | GCATC | 2 cut(s) | 784, 899 |
| MaeI | CTAG | 2 cut(s) | 782, 815 |
| MaeII | ACGT | 2 cut(s) | 73, 809 |
| MaeIII | GTNAC | 2 cut(s) | 810, 1026 |
| MalI | GATC | 2 cut(s) | 23, 938 |
| MbiI | CCGCTC | 4 cut(s) | 80, 399, 465, 540 |
| MboI | GATC | 2 cut(s) | 21, 936 |
| MboII | GAAGA | 5 cut(s) | 1058, 1091, 1094, 1097, 1172 |
| MflI | RGATCY | 1 cut(s) | 936 |
| MhlI | GDGCHC | 1 cut(s) | 35 |
| MluCI | AATT | 5 cut(s) | 181, 334, 607, 673, 739 |
| MmeI | TCCRAC | 1 cut(s) | 33 |
| MspR9I | CCNGG | 1 cut(s) | 596 |
| MvaI | CCWGG | 1 cut(s) | 596 |
| MvnI | CGCG | 1 cut(s) | 14 |
| NcoI | CCATGG | 1 cut(s) | 773 |
| NdeII | GATC | 2 cut(s) | 21, 936 |
| NlaIII | CATG | 3 cut(s) | 203, 777, 1216 |
| NlaIV | GGNNCC | 2 cut(s) | 938, 1062 |
| NmuCI | GTSAC | 1 cut(s) | 810 |
| PceI | AGGCCT | 1 cut(s) | 899 |
| PflFI | GACNNNGTC | 1 cut(s) | 153 |
| PkrI | GCNGC | 5 cut(s) | 206, 209, 720, 771, 806 |
| PmaCI | CACGTG | 1 cut(s) | 74 |
| PmlI | CACGTG | 1 cut(s) | 74 |
| Ppu21I | YACGTR | 1 cut(s) | 74 |
| Psp124BI | GAGCTC | 1 cut(s) | 35 |
| Psp6I | CCWGG | 1 cut(s) | 594 |
| PspCI | CACGTG | 1 cut(s) | 74 |
| PspGI | CCWGG | 1 cut(s) | 594 |
| PspN4I | GGNNCC | 2 cut(s) | 938, 1062 |
| PstI | CTGCAG | 1 cut(s) | 723 |
| PstNI | CAGNNNCTG | 1 cut(s) | 727 |
| PsuI | RGATCY | 1 cut(s) | 936 |
| PsyI | GACNNNGTC | 1 cut(s) | 153 |
| RsaI | GTAC | 4 cut(s) | 320, 392, 458, 659 |
| RsaNI | GTAC | 4 cut(s) | 319, 391, 457, 658 |
| SacI | GAGCTC | 1 cut(s) | 35 |
| SatI | GCNGC | 5 cut(s) | 205, 208, 719, 770, 805 |
| Sau3AI | GATC | 2 cut(s) | 21, 936 |
| ScaI | AGTACT | 1 cut(s) | 320 |
| ScrFI | CCNGG | 1 cut(s) | 596 |
| SduI | GDGCHC | 1 cut(s) | 35 |
| SfaNI | GCATC | 2 cut(s) | 784, 899 |
| SfcI | CTRYAG | 2 cut(s) | 53, 719 |
| SmlI | CTYRAG | 8 cut(s) | 215, 328, 394, 460, 601, 620, 667, 826 |
| SmoI | CTYRAG | 8 cut(s) | 215, 328, 394, 460, 601, 620, 667, 826 |
| Sse9I | AATT | 5 cut(s) | 181, 334, 607, 673, 739 |
| SseBI | AGGCCT | 1 cut(s) | 899 |
| SsiI | CCGC | 8 cut(s) | 14, 63, 80, 120, 207, 399, 465, 540 |
| SspMI | CTAG | 2 cut(s) | 782, 815 |
| SstI | GAGCTC | 1 cut(s) | 35 |
| StuI | AGGCCT | 1 cut(s) | 899 |
| StyD4I | CCNGG | 1 cut(s) | 594 |
| StyI | CCWWGG | 1 cut(s) | 773 |
| TaaI | ACNGT | 3 cut(s) | 47, 823, 973 |
| TaiI | ACGT | 2 cut(s) | 76, 812 |
| TasI | AATT | 5 cut(s) | 181, 334, 607, 673, 739 |
| TatI | WGTACW | 3 cut(s) | 318, 390, 456 |
| TauI | GCSGC | 1 cut(s) | 210 |
| TscAI | CASTG | 3 cut(s) | 43, 976, 1138 |
| TseFI | GTSAC | 1 cut(s) | 810 |
| TseI | GCWGC | 4 cut(s) | 204, 718, 769, 804 |
| Tsp45I | GTSAC | 1 cut(s) | 810 |
| TspDTI | ATGAA | 2 cut(s) | 1059, 1065 |
| TspRI | CASTG | 3 cut(s) | 43, 976, 1138 |
| Tth111I | GACNNNGTC | 1 cut(s) | 153 |
| XapI | RAATTY | 1 cut(s) | 181 |
| XspI | CTAG | 2 cut(s) | 782, 815 |
| ZrmI | AGTACT | 1 cut(s) | 320 |