protein serine/threonine kinase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 635 |
| Acc36I | ACCTGC | 1 cut(s) | 66 |
| AccB1I | GGYRCC | 1 cut(s) | 191 |
| AcoI | YGGCCR | 2 cut(s) | 418, 775 |
| AcsI | RAATTY | 2 cut(s) | 227, 347 |
| AcuI | CTGAAG | 4 cut(s) | 291, 312, 450, 813 |
| AfaI | GTAC | 1 cut(s) | 487 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 661, 702 |
| AgsI | TTSAA | 4 cut(s) | 112, 199, 500, 761 |
| AjnI | CCWGG | 2 cut(s) | 61, 187 |
| AluBI | AGCT | 5 cut(s) | 373, 403, 510, 535, 827 |
| AluI | AGCT | 5 cut(s) | 373, 403, 510, 535, 827 |
| Alw26I | GTCTC | 1 cut(s) | 281 |
| AoxI | GGCC | 2 cut(s) | 418, 775 |
| ApeKI | GCWGC | 3 cut(s) | 13, 72, 547 |
| ApoI | RAATTY | 2 cut(s) | 227, 347 |
| AsuC2I | CCSGG | 1 cut(s) | 696 |
| BalI | TGGCCA | 2 cut(s) | 420, 777 |
| BanI | GGYRCC | 1 cut(s) | 191 |
| BbvI | GCAGC | 2 cut(s) | 84, 534 |
| BccI | CCATC | 3 cut(s) | 41, 115, 349 |
| BciT130I | CCWGG | 2 cut(s) | 63, 189 |
| BcnI | CCSGG | 1 cut(s) | 696 |
| BcoDI | GTCTC | 1 cut(s) | 281 |
| BfaI | CTAG | 3 cut(s) | 365, 507, 824 |
| BfmI | CTRYAG | 1 cut(s) | 478 |
| BfuAI | ACCTGC | 1 cut(s) | 66 |
| BglII | AGATCT | 2 cut(s) | 472, 805 |
| BisI | GCNGC | 3 cut(s) | 14, 73, 548 |
| BlsI | GCNGC | 3 cut(s) | 15, 74, 549 |
| Bme1390I | CCNGG | 3 cut(s) | 63, 189, 696 |
| BmiI | GGNNCC | 1 cut(s) | 193 |
| BmrFI | CCNGG | 3 cut(s) | 63, 189, 696 |
| BmsI | GCATC | 1 cut(s) | 699 |
| BpmI | CTGGAG | 1 cut(s) | 191 |
| BpuEI | CTTGAG | 1 cut(s) | 263 |
| BpuMI | CCSGG | 1 cut(s) | 696 |
| BsaBI | GATNNNNATC | 1 cut(s) | 99 |
| BsaI | GGTCTC | 1 cut(s) | 281 |
| BsaJI | CCNNGG | 2 cut(s) | 61, 90 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 661, 702 |
| Bse118I | RCCGGY | 1 cut(s) | 609 |
| Bse1I | ACTGG | 1 cut(s) | 421 |
| Bse8I | GATNNNNATC | 1 cut(s) | 99 |
| BseBI | CCWGG | 2 cut(s) | 63, 189 |
| BseDI | CCNNGG | 2 cut(s) | 61, 90 |
| BseGI | GGATG | 5 cut(s) | 100, 126, 714, 741, 751 |
| BseJI | GATNNNNATC | 1 cut(s) | 99 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 661, 702 |
| BseMII | CTCAG | 3 cut(s) | 173, 461, 722 |
| BseNI | ACTGG | 1 cut(s) | 421 |
| BseXI | GCAGC | 2 cut(s) | 84, 534 |
| BsgI | GTGCAG | 1 cut(s) | 219 |
| BshFI | GGCC | 2 cut(s) | 420, 777 |
| BshNI | GGYRCC | 1 cut(s) | 191 |
| BsiSI | CCGG | 2 cut(s) | 610, 696 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 661, 702 |
| BsmAI | GTCTC | 1 cut(s) | 281 |
| BsmI | GAATGC | 1 cut(s) | 91 |
| BsnI | GGCC | 2 cut(s) | 420, 777 |
| Bso31I | GGTCTC | 1 cut(s) | 281 |
| Bsp1407I | TGTACA | 1 cut(s) | 485 |
| Bsp143I | GATC | 3 cut(s) | 439, 472, 805 |
| BspANI | GGCC | 2 cut(s) | 420, 777 |
| BspCNI | CTCAG | 3 cut(s) | 174, 460, 723 |
| BspHI | TCATGA | 1 cut(s) | 40 |
| BspLI | GGNNCC | 1 cut(s) | 193 |
| BspMI | ACCTGC | 1 cut(s) | 66 |
| BspT107I | GGYRCC | 1 cut(s) | 191 |
| BspTNI | GGTCTC | 1 cut(s) | 281 |
| BsrFI | RCCGGY | 1 cut(s) | 609 |
| BsrGI | TGTACA | 1 cut(s) | 485 |
| BsrI | ACTGG | 1 cut(s) | 421 |
| BssAI | RCCGGY | 1 cut(s) | 609 |
| BssECI | CCNNGG | 2 cut(s) | 61, 90 |
| BssMI | GATC | 3 cut(s) | 439, 472, 805 |
| BssT1I | CCWWGG | 1 cut(s) | 90 |
| Bst2UI | CCWGG | 2 cut(s) | 63, 189 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 10 |
| BstAUI | TGTACA | 1 cut(s) | 485 |
| BstC8I | GCNNGC | 1 cut(s) | 552 |
| BstDEI | CTNAG | 5 cut(s) | 182, 215, 299, 447, 731 |
| BstF5I | GGATG | 5 cut(s) | 100, 126, 714, 741, 751 |
| BstKTI | GATC | 3 cut(s) | 442, 475, 808 |
| BstMAI | GTCTC | 1 cut(s) | 281 |
| BstMBI | GATC | 3 cut(s) | 439, 472, 805 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 10, 379 |
| BstNI | CCWGG | 2 cut(s) | 63, 189 |
| BstNSI | RCATGY | 2 cut(s) | 382, 617 |
| BstSCI | CCNGG | 3 cut(s) | 61, 187, 694 |
| BstSFI | CTRYAG | 1 cut(s) | 478 |
| BstV1I | GCAGC | 2 cut(s) | 84, 534 |
| BstX2I | RGATCY | 2 cut(s) | 472, 805 |
| BstYI | RGATCY | 2 cut(s) | 472, 805 |
| BsuRI | GGCC | 2 cut(s) | 420, 777 |
| BtsCI | GGATG | 5 cut(s) | 100, 126, 714, 741, 751 |
| BtsIMutI | CAGTG | 2 cut(s) | 455, 728 |
| BveI | ACCTGC | 1 cut(s) | 66 |
| Cac8I | GCNNGC | 1 cut(s) | 552 |
| CciI | TCATGA | 1 cut(s) | 40 |
| Cfr10I | RCCGGY | 1 cut(s) | 609 |
| Csp6I | GTAC | 1 cut(s) | 486 |
| CviAII | CATG | 4 cut(s) | 5, 41, 379, 614 |
| CviJI | RGCY | 9 cut(s) | 143, 373, 403, 420, 510, 535, 585, 777, 827 |
| CviKI_1 | RGCY | 9 cut(s) | 143, 373, 403, 420, 510, 535, 585, 777, 827 |
| CviQI | GTAC | 1 cut(s) | 486 |
| DdeI | CTNAG | 5 cut(s) | 182, 215, 299, 447, 731 |
| DpnI | GATC | 3 cut(s) | 441, 474, 807 |
| DpnII | GATC | 3 cut(s) | 439, 472, 805 |
| DraI | TTTAAA | 1 cut(s) | 520 |
| EaeI | YGGCCR | 2 cut(s) | 418, 775 |
| Eco130I | CCWWGG | 1 cut(s) | 90 |
| Eco31I | GGTCTC | 1 cut(s) | 281 |
| Eco32I | GATATC | 1 cut(s) | 305 |
| Eco57I | CTGAAG | 4 cut(s) | 291, 312, 450, 813 |
| EcoRII | CCWGG | 2 cut(s) | 61, 187 |
| EcoRV | GATATC | 1 cut(s) | 305 |
| EcoT14I | CCWWGG | 1 cut(s) | 90 |
| EcoT22I | ATGCAT | 1 cut(s) | 6 |
| ErhI | CCWWGG | 1 cut(s) | 90 |
| FaeI | CATG | 4 cut(s) | 8, 44, 382, 617 |
| FatI | CATG | 4 cut(s) | 4, 40, 378, 613 |
| Fnu4HI | GCNGC | 3 cut(s) | 14, 73, 548 |
| FokI | GGATG | 5 cut(s) | 107, 133, 721, 748, 758 |
| Fsp4HI | GCNGC | 3 cut(s) | 14, 73, 548 |
| FspBI | CTAG | 3 cut(s) | 365, 507, 824 |
| GluI | GCNGC | 3 cut(s) | 14, 73, 548 |
| GsuI | CTGGAG | 1 cut(s) | 191 |
| HaeIII | GGCC | 2 cut(s) | 420, 777 |
| HapII | CCGG | 2 cut(s) | 610, 696 |
| Hin1II | CATG | 4 cut(s) | 8, 44, 382, 617 |
| HincII | GTYRAC | 2 cut(s) | 426, 645 |
| HindII | GTYRAC | 2 cut(s) | 426, 645 |
| HindIII | AAGCTT | 2 cut(s) | 401, 533 |
| HinfI | GANTC | 3 cut(s) | 184, 650, 796 |
| HpaI | GTTAAC | 1 cut(s) | 645 |
| HpaII | CCGG | 2 cut(s) | 610, 696 |
| Hpy166II | GTNNAC | 4 cut(s) | 250, 426, 486, 645 |
| Hpy188I | TCNGA | 3 cut(s) | 154, 346, 724 |
| Hpy188III | TCNNGA | 6 cut(s) | 41, 170, 269, 290, 437, 527 |
| Hpy8I | GTNNAC | 4 cut(s) | 250, 426, 486, 645 |
| HpyAV | CCTTC | 4 cut(s) | 205, 266, 275, 336 |
| HpyCH4V | TGCA | 7 cut(s) | 4, 16, 51, 236, 262, 572, 661 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 10, 379 |
| HpyF3I | CTNAG | 5 cut(s) | 182, 215, 299, 447, 731 |
| Hsp92II | CATG | 4 cut(s) | 8, 44, 382, 617 |
| KspAI | GTTAAC | 1 cut(s) | 645 |
| Kzo9I | GATC | 3 cut(s) | 439, 472, 805 |
| Lsp1109I | GCAGC | 2 cut(s) | 84, 534 |
| LweI | GCATC | 1 cut(s) | 699 |
| MaeI | CTAG | 3 cut(s) | 365, 507, 824 |
| MaeIII | GTNAC | 1 cut(s) | 174 |
| MalI | GATC | 3 cut(s) | 441, 474, 807 |
| MboI | GATC | 3 cut(s) | 439, 472, 805 |
| MboII | GAAGA | 2 cut(s) | 305, 800 |
| MflI | RGATCY | 2 cut(s) | 472, 805 |
| MlsI | TGGCCA | 2 cut(s) | 420, 777 |
| MluCI | AATT | 6 cut(s) | 227, 347, 513, 636, 763, 779 |
| MluNI | TGGCCA | 2 cut(s) | 420, 777 |
| MlyI | GAGTC | 2 cut(s) | 193, 805 |
| MmeI | TCCRAC | 1 cut(s) | 720 |
| MnlI | CCTC | 6 cut(s) | 457, 572, 658, 699, 726, 736 |
| Mox20I | TGGCCA | 2 cut(s) | 420, 777 |
| Mph1103I | ATGCAT | 1 cut(s) | 6 |
| MscI | TGGCCA | 2 cut(s) | 420, 777 |
| MseI | TTAA | 3 cut(s) | 396, 519, 644 |
| MslI | CAYNNNNRTG | 2 cut(s) | 414, 618 |
| Msp20I | TGGCCA | 2 cut(s) | 420, 777 |
| MspI | CCGG | 2 cut(s) | 610, 696 |
| MspR9I | CCNGG | 3 cut(s) | 63, 189, 696 |
| Mva1269I | GAATGC | 1 cut(s) | 91 |
| MvaI | CCWGG | 2 cut(s) | 63, 189 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 10, 379 |
| NciI | CCSGG | 1 cut(s) | 696 |
| NdeII | GATC | 3 cut(s) | 439, 472, 805 |
| NlaIII | CATG | 4 cut(s) | 8, 44, 382, 617 |
| NlaIV | GGNNCC | 1 cut(s) | 193 |
| NsiI | ATGCAT | 1 cut(s) | 6 |
| NspI | RCATGY | 2 cut(s) | 382, 617 |
| PagI | TCATGA | 1 cut(s) | 40 |
| PctI | GAATGC | 1 cut(s) | 91 |
| PfeI | GAWTC | 1 cut(s) | 650 |
| PfoI | TCCNGGA | 1 cut(s) | 694 |
| PkrI | GCNGC | 3 cut(s) | 15, 74, 549 |
| PleI | GAGTC | 2 cut(s) | 192, 804 |
| PpsI | GAGTC | 2 cut(s) | 192, 804 |
| PsiI | TTATAA | 1 cut(s) | 635 |
| Psp6I | CCWGG | 2 cut(s) | 61, 187 |
| PspGI | CCWGG | 2 cut(s) | 61, 187 |
| PspN4I | GGNNCC | 1 cut(s) | 193 |
| PsuI | RGATCY | 2 cut(s) | 472, 805 |
| RsaI | GTAC | 1 cut(s) | 487 |
| RsaNI | GTAC | 1 cut(s) | 486 |
| RseI | CAYNNNNRTG | 2 cut(s) | 414, 618 |
| SaqAI | TTAA | 3 cut(s) | 396, 519, 644 |
| SatI | GCNGC | 3 cut(s) | 14, 73, 548 |
| Sau3AI | GATC | 3 cut(s) | 439, 472, 805 |
| SchI | GAGTC | 2 cut(s) | 193, 805 |
| ScrFI | CCNGG | 3 cut(s) | 63, 189, 696 |
| SfaNI | GCATC | 1 cut(s) | 699 |
| SfcI | CTRYAG | 1 cut(s) | 478 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 414, 618 |
| SmlI | CTYRAG | 1 cut(s) | 278 |
| SmoI | CTYRAG | 1 cut(s) | 278 |
| Sse9I | AATT | 6 cut(s) | 227, 347, 513, 636, 763, 779 |
| SspI | AATATT | 1 cut(s) | 460 |
| SspMI | CTAG | 3 cut(s) | 365, 507, 824 |
| StyD4I | CCNGG | 3 cut(s) | 61, 187, 694 |
| StyI | CCWWGG | 1 cut(s) | 90 |
| TasI | AATT | 6 cut(s) | 227, 347, 513, 636, 763, 779 |
| TatI | WGTACW | 1 cut(s) | 485 |
| TfiI | GAWTC | 1 cut(s) | 650 |
| Tru1I | TTAA | 3 cut(s) | 396, 519, 644 |
| Tru9I | TTAA | 3 cut(s) | 396, 519, 644 |
| TscAI | CASTG | 2 cut(s) | 455, 735 |
| TseI | GCWGC | 3 cut(s) | 13, 72, 547 |
| TspDTI | ATGAA | 3 cut(s) | 29, 57, 636 |
| TspGWI | ACGGA | 1 cut(s) | 662 |
| TspRI | CASTG | 2 cut(s) | 455, 735 |
| XapI | RAATTY | 2 cut(s) | 227, 347 |
| XceI | RCATGY | 2 cut(s) | 382, 617 |
| XspI | CTAG | 3 cut(s) | 365, 507, 824 |
| Zsp2I | ATGCAT | 1 cut(s) | 6 |