RESISTANCE protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1647 |
| Acc65I | GGTACC | 1 cut(s) | 853 |
| AccB1I | GGYRCC | 2 cut(s) | 853, 1814 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 178 |
| AccI | GTMKAC | 1 cut(s) | 913 |
| AciI | CCGC | 2 cut(s) | 702, 1834 |
| AclWI | GGATC | 1 cut(s) | 233 |
| AcsI | RAATTY | 6 cut(s) | 128, 327, 826, 1422, 1447, 1703 |
| AcuI | CTGAAG | 2 cut(s) | 276, 1139 |
| AfaI | GTAC | 6 cut(s) | 167, 649, 838, 855, 1538, 1737 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 178 |
| AflII | CTTAAG | 1 cut(s) | 524 |
| AjnI | CCWGG | 1 cut(s) | 617 |
| AluBI | AGCT | 8 cut(s) | 93, 358, 553, 613, 1013, 1072, 1353, 1679 |
| AluI | AGCT | 8 cut(s) | 93, 358, 553, 613, 1013, 1072, 1353, 1679 |
| Alw21I | GWGCWC | 2 cut(s) | 1074, 1681 |
| Alw26I | GTCTC | 5 cut(s) | 37, 483, 565, 701, 743 |
| AlwI | GGATC | 1 cut(s) | 233 |
| Ama87I | CYCGRG | 3 cut(s) | 404, 562, 1179 |
| AoxI | GGCC | 1 cut(s) | 151 |
| ApeKI | GCWGC | 3 cut(s) | 585, 1013, 1831 |
| ApoI | RAATTY | 6 cut(s) | 128, 327, 826, 1422, 1447, 1703 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1433 |
| Asp718I | GGTACC | 1 cut(s) | 853 |
| AspLEI | GCGC | 1 cut(s) | 883 |
| AsuHPI | GGTGA | 5 cut(s) | 691, 1265, 1614, 1723, 1789 |
| AsuII | TTCGAA | 1 cut(s) | 886 |
| AvaI | CYCGRG | 3 cut(s) | 404, 562, 1179 |
| AxyI | CCTNAGG | 1 cut(s) | 1626 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 615, 648, 828, 861 |
| BanI | GGYRCC | 2 cut(s) | 853, 1814 |
| BanII | GRGCYC | 2 cut(s) | 1074, 1681 |
| BbsI | GAAGAC | 3 cut(s) | 204, 524, 1127 |
| Bbv12I | GWGCWC | 2 cut(s) | 1074, 1681 |
| BbvI | GCAGC | 3 cut(s) | 572, 1000, 1843 |
| BccI | CCATC | 4 cut(s) | 130, 1333, 1587, 1742 |
| BceAI | ACGGC | 1 cut(s) | 1551 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 1129, 1163, 1231, 1265, 1272, 1306 |
| BciT130I | CCWGG | 1 cut(s) | 619 |
| BciVI | GTATCC | 2 cut(s) | 616, 1604 |
| BcoDI | GTCTC | 5 cut(s) | 37, 483, 565, 701, 743 |
| BfaI | CTAG | 3 cut(s) | 578, 635, 875 |
| BfrI | CTTAAG | 1 cut(s) | 524 |
| BfuI | GTATCC | 2 cut(s) | 616, 1604 |
| BglII | AGATCT | 1 cut(s) | 1388 |
| BisI | GCNGC | 3 cut(s) | 586, 1014, 1832 |
| BlsI | GCNGC | 3 cut(s) | 587, 1015, 1833 |
| Bme1390I | CCNGG | 1 cut(s) | 619 |
| BmeT110I | CYCGRG | 3 cut(s) | 404, 562, 1179 |
| BmiI | GGNNCC | 2 cut(s) | 855, 1816 |
| BmrFI | CCNGG | 1 cut(s) | 619 |
| BmsI | GCATC | 5 cut(s) | 230, 399, 1246, 1298, 1520 |
| BoxI | GACNNNNGTC | 1 cut(s) | 204 |
| BpiI | GAAGAC | 3 cut(s) | 204, 524, 1127 |
| BpmI | CTGGAG | 2 cut(s) | 791, 836 |
| Bpu14I | TTCGAA | 1 cut(s) | 886 |
| BsaAI | YACGTR | 1 cut(s) | 995 |
| BsaI | GGTCTC | 2 cut(s) | 483, 701 |
| BsaJI | CCNNGG | 5 cut(s) | 209, 373, 617, 618, 1583 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1056, 1086, 1754, 1784 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 178 |
| Bse1I | ACTGG | 1 cut(s) | 1612 |
| Bse21I | CCTNAGG | 1 cut(s) | 1626 |
| Bse3DI | GCAATG | 2 cut(s) | 379, 1094 |
| BseBI | CCWGG | 1 cut(s) | 619 |
| BseDI | CCNNGG | 5 cut(s) | 209, 373, 617, 618, 1583 |
| BseGI | GGATG | 4 cut(s) | 245, 1231, 1535, 1579 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 178 |
| BseMI | GCAATG | 2 cut(s) | 379, 1094 |
| BseMII | CTCAG | 4 cut(s) | 810, 1279, 1617, 1704 |
| BseNI | ACTGG | 1 cut(s) | 1612 |
| BseRI | GAGGAG | 4 cut(s) | 1772, 1775, 1778, 1781 |
| BseXI | GCAGC | 3 cut(s) | 572, 1000, 1843 |
| BsgI | GTGCAG | 2 cut(s) | 928, 1128 |
| BshFI | GGCC | 1 cut(s) | 153 |
| BshNI | GGYRCC | 2 cut(s) | 853, 1814 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1074, 1681 |
| BsiHKCI | CYCGRG | 3 cut(s) | 404, 562, 1179 |
| BslFI | GGGAC | 1 cut(s) | 245 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 178 |
| BsmAI | GTCTC | 5 cut(s) | 37, 483, 565, 701, 743 |
| BsmFI | GGGAC | 1 cut(s) | 245 |
| BsmI | GAATGC | 1 cut(s) | 1237 |
| BsnI | GGCC | 1 cut(s) | 153 |
| Bso31I | GGTCTC | 2 cut(s) | 483, 701 |
| BsoBI | CYCGRG | 3 cut(s) | 404, 562, 1179 |
| Bsp119I | TTCGAA | 1 cut(s) | 886 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1074, 1681 |
| Bsp143I | GATC | 4 cut(s) | 14, 64, 225, 1388 |
| BspACI | CCGC | 2 cut(s) | 702, 1834 |
| BspANI | GGCC | 1 cut(s) | 153 |
| BspCNI | CTCAG | 4 cut(s) | 809, 1278, 1618, 1705 |
| BspHI | TCATGA | 2 cut(s) | 481, 1189 |
| BspLI | GGNNCC | 2 cut(s) | 855, 1816 |
| BspPI | GGATC | 1 cut(s) | 233 |
| BspT104I | TTCGAA | 1 cut(s) | 886 |
| BspT107I | GGYRCC | 2 cut(s) | 853, 1814 |
| BspTI | CTTAAG | 1 cut(s) | 524 |
| BspTNI | GGTCTC | 2 cut(s) | 483, 701 |
| BsrDI | GCAATG | 2 cut(s) | 379, 1094 |
| BsrI | ACTGG | 1 cut(s) | 1612 |
| BssECI | CCNNGG | 5 cut(s) | 209, 373, 617, 618, 1583 |
| BssMI | GATC | 4 cut(s) | 14, 64, 225, 1388 |
| BssNAI | GTATAC | 1 cut(s) | 914 |
| BssT1I | CCWWGG | 3 cut(s) | 209, 373, 1583 |
| Bst1107I | GTATAC | 1 cut(s) | 914 |
| Bst2UI | CCWGG | 1 cut(s) | 619 |
| Bst4CI | ACNGT | 5 cut(s) | 311, 631, 1043, 1126, 1735 |
| BstAFI | CTTAAG | 1 cut(s) | 524 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 546 |
| BstBAI | YACGTR | 1 cut(s) | 995 |
| BstBI | TTCGAA | 1 cut(s) | 886 |
| BstC8I | GCNNGC | 4 cut(s) | 356, 551, 1000, 1836 |
| BstF5I | GGATG | 4 cut(s) | 245, 1231, 1535, 1579 |
| BstHHI | GCGC | 1 cut(s) | 883 |
| BstKTI | GATC | 4 cut(s) | 17, 67, 228, 1391 |
| BstMAI | GTCTC | 5 cut(s) | 37, 483, 565, 701, 743 |
| BstMBI | GATC | 4 cut(s) | 14, 64, 225, 1388 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 546, 1844 |
| BstNI | CCWGG | 1 cut(s) | 619 |
| BstNSI | RCATGY | 2 cut(s) | 26, 30 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 204 |
| BstSCI | CCNGG | 1 cut(s) | 617 |
| BstV1I | GCAGC | 3 cut(s) | 572, 1000, 1843 |
| BstV2I | GAAGAC | 3 cut(s) | 204, 524, 1127 |
| BstX2I | RGATCY | 1 cut(s) | 1388 |
| BstYI | RGATCY | 1 cut(s) | 1388 |
| BstZ17I | GTATAC | 1 cut(s) | 914 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1626 |
| BsuI | GTATCC | 2 cut(s) | 616, 1604 |
| BsuRI | GGCC | 1 cut(s) | 153 |
| BtsCI | GGATG | 4 cut(s) | 245, 1231, 1535, 1579 |
| BtsI | GCAGTG | 2 cut(s) | 1140, 1689 |
| BtsIMutI | CAGTG | 5 cut(s) | 636, 1115, 1131, 1140, 1689 |
| Cac8I | GCNNGC | 4 cut(s) | 356, 551, 1000, 1836 |
| CciI | TCATGA | 2 cut(s) | 481, 1189 |
| CfoI | GCGC | 1 cut(s) | 883 |
| CseI | GACGC | 1 cut(s) | 991 |
| Csp6I | GTAC | 6 cut(s) | 166, 648, 837, 854, 1537, 1736 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 217, 252 |
| CviAII | CATG | 6 cut(s) | 23, 27, 414, 482, 898, 1190 |
| CviQI | GTAC | 6 cut(s) | 166, 648, 837, 854, 1537, 1736 |
| DpnI | GATC | 4 cut(s) | 16, 66, 227, 1390 |
| DpnII | GATC | 4 cut(s) | 14, 64, 225, 1388 |
| DraI | TTTAAA | 1 cut(s) | 1303 |
| Ecl136II | GAGCTC | 2 cut(s) | 1072, 1679 |
| Eco130I | CCWWGG | 3 cut(s) | 209, 373, 1583 |
| Eco24I | GRGCYC | 2 cut(s) | 1074, 1681 |
| Eco31I | GGTCTC | 2 cut(s) | 483, 701 |
| Eco53kI | GAGCTC | 2 cut(s) | 1072, 1679 |
| Eco57I | CTGAAG | 2 cut(s) | 276, 1139 |
| Eco81I | CCTNAGG | 1 cut(s) | 1626 |
| Eco88I | CYCGRG | 3 cut(s) | 404, 562, 1179 |
| EcoICRI | GAGCTC | 2 cut(s) | 1072, 1679 |
| EcoRII | CCWGG | 1 cut(s) | 617 |
| EcoT14I | CCWWGG | 3 cut(s) | 209, 373, 1583 |
| EcoT22I | ATGCAT | 1 cut(s) | 28 |
| EcoT38I | GRGCYC | 2 cut(s) | 1074, 1681 |
| ErhI | CCWWGG | 3 cut(s) | 209, 373, 1583 |
| FaeI | CATG | 6 cut(s) | 26, 30, 417, 485, 901, 1193 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 529, 561, 1526, 1558 |
| FaqI | GGGAC | 1 cut(s) | 245 |
| FatI | CATG | 6 cut(s) | 22, 26, 413, 481, 897, 1189 |
| FauI | CCCGC | 1 cut(s) | 1827 |
| FauNDI | CATATG | 2 cut(s) | 1330, 1345 |
| FblI | GTMKAC | 1 cut(s) | 913 |
| Fnu4HI | GCNGC | 3 cut(s) | 586, 1014, 1832 |
| FokI | GGATG | 4 cut(s) | 252, 1238, 1542, 1566 |
| FriOI | GRGCYC | 2 cut(s) | 1074, 1681 |
| Fsp4HI | GCNGC | 3 cut(s) | 586, 1014, 1832 |
| FspBI | CTAG | 3 cut(s) | 578, 635, 875 |
| GlaI | GCGC | 1 cut(s) | 882 |
| GluI | GCNGC | 3 cut(s) | 586, 1014, 1832 |
| GsuI | CTGGAG | 2 cut(s) | 791, 836 |
| HaeIII | GGCC | 1 cut(s) | 153 |
| HgaI | GACGC | 1 cut(s) | 991 |
| HhaI | GCGC | 1 cut(s) | 883 |
| Hin1II | CATG | 6 cut(s) | 26, 30, 417, 485, 901, 1193 |
| Hin6I | GCGC | 1 cut(s) | 881 |
| HinP1I | GCGC | 1 cut(s) | 881 |
| HincII | GTYRAC | 1 cut(s) | 1726 |
| HindII | GTYRAC | 1 cut(s) | 1726 |
| HindIII | AAGCTT | 1 cut(s) | 356 |
| HphI | GGTGA | 5 cut(s) | 691, 1265, 1614, 1723, 1789 |
| Hpy166II | GTNNAC | 4 cut(s) | 518, 914, 1576, 1726 |
| Hpy188I | TCNGA | 7 cut(s) | 276, 799, 1210, 1268, 1297, 1339, 1428 |
| Hpy8I | GTNNAC | 4 cut(s) | 518, 914, 1576, 1726 |
| HpyAV | CCTTC | 2 cut(s) | 342, 1301 |
| HpyCH4III | ACNGT | 5 cut(s) | 311, 631, 1043, 1126, 1735 |
| HpyCH4IV | ACGT | 1 cut(s) | 994 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 546, 1844 |
| HpySE526I | ACGT | 1 cut(s) | 994 |
| Hsp92II | CATG | 6 cut(s) | 26, 30, 417, 485, 901, 1193 |
| HspAI | GCGC | 1 cut(s) | 881 |
| KpnI | GGTACC | 1 cut(s) | 857 |
| Kzo9I | GATC | 4 cut(s) | 14, 64, 225, 1388 |
| LmnI | GCTCC | 4 cut(s) | 610, 1077, 1239, 1676 |
| Lsp1109I | GCAGC | 3 cut(s) | 572, 1000, 1843 |
| LweI | GCATC | 5 cut(s) | 230, 399, 1246, 1298, 1520 |
| MaeI | CTAG | 3 cut(s) | 578, 635, 875 |
| MaeII | ACGT | 1 cut(s) | 994 |
| MaeIII | GTNAC | 2 cut(s) | 1113, 1708 |
| MalI | GATC | 4 cut(s) | 16, 66, 227, 1390 |
| MboI | GATC | 4 cut(s) | 14, 64, 225, 1388 |
| MboII | GAAGA | 6 cut(s) | 59, 209, 392, 524, 1132, 1673 |
| MfeI | CAATTG | 2 cut(s) | 718, 864 |
| MflI | RGATCY | 1 cut(s) | 1388 |
| MhlI | GDGCHC | 2 cut(s) | 1074, 1681 |
| MlyI | GAGTC | 5 cut(s) | 214, 286, 538, 692, 745 |
| MmeI | TCCRAC | 3 cut(s) | 1203, 1507, 1752 |
| Mph1103I | ATGCAT | 1 cut(s) | 28 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1433 |
| MslI | CAYNNNNRTG | 2 cut(s) | 27, 309 |
| MspA1I | CMGCKG | 1 cut(s) | 1834 |
| MspCI | CTTAAG | 1 cut(s) | 524 |
| MspR9I | CCNGG | 1 cut(s) | 619 |
| MunI | CAATTG | 2 cut(s) | 718, 864 |
| Mva1269I | GAATGC | 1 cut(s) | 1237 |
| MvaI | CCWGG | 1 cut(s) | 619 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 546, 1844 |
| NdeI | CATATG | 2 cut(s) | 1330, 1345 |
| NdeII | GATC | 4 cut(s) | 14, 64, 225, 1388 |
| NlaIII | CATG | 6 cut(s) | 26, 30, 417, 485, 901, 1193 |
| NlaIV | GGNNCC | 2 cut(s) | 855, 1816 |
| NmeAIII | GCCGAG | 1 cut(s) | 1843 |
| NmuCI | GTSAC | 1 cut(s) | 1113 |
| NsiI | ATGCAT | 1 cut(s) | 28 |
| NspI | RCATGY | 2 cut(s) | 26, 30 |
| NspV | TTCGAA | 1 cut(s) | 886 |
| PaeR7I | CTCGAG | 2 cut(s) | 562, 1179 |
| PagI | TCATGA | 2 cut(s) | 481, 1189 |
| PasI | CCCWGGG | 1 cut(s) | 618 |
| PctI | GAATGC | 1 cut(s) | 1237 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1433 |
| PfeI | GAWTC | 6 cut(s) | 590, 674, 1058, 1334, 1340, 1619 |
| PflFI | GACNNNGTC | 1 cut(s) | 1126 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 178 |
| PkrI | GCNGC | 3 cut(s) | 587, 1015, 1833 |
| PleI | GAGTC | 5 cut(s) | 213, 285, 537, 691, 744 |
| PpsI | GAGTC | 5 cut(s) | 213, 285, 537, 691, 744 |
| Ppu21I | YACGTR | 1 cut(s) | 995 |
| PshAI | GACNNNNGTC | 1 cut(s) | 204 |
| PsiI | TTATAA | 1 cut(s) | 1647 |
| Psp124BI | GAGCTC | 2 cut(s) | 1074, 1681 |
| Psp6I | CCWGG | 1 cut(s) | 617 |
| PspGI | CCWGG | 1 cut(s) | 617 |
| PspN4I | GGNNCC | 2 cut(s) | 855, 1816 |
| PsuI | RGATCY | 1 cut(s) | 1388 |
| PsyI | GACNNNGTC | 1 cut(s) | 1126 |
| RsaI | GTAC | 6 cut(s) | 167, 649, 838, 855, 1538, 1737 |
| RsaNI | GTAC | 6 cut(s) | 166, 648, 837, 854, 1537, 1736 |
| RseI | CAYNNNNRTG | 2 cut(s) | 27, 309 |
| SacI | GAGCTC | 2 cut(s) | 1074, 1681 |
| SatI | GCNGC | 3 cut(s) | 586, 1014, 1832 |
| Sau3AI | GATC | 4 cut(s) | 14, 64, 225, 1388 |
| SchI | GAGTC | 5 cut(s) | 214, 286, 538, 692, 745 |
| ScrFI | CCNGG | 1 cut(s) | 619 |
| SduI | GDGCHC | 2 cut(s) | 1074, 1681 |
| SfaNI | GCATC | 5 cut(s) | 230, 399, 1246, 1298, 1520 |
| Sfr274I | CTCGAG | 2 cut(s) | 562, 1179 |
| SfuI | TTCGAA | 1 cut(s) | 886 |
| SlaI | CTCGAG | 2 cut(s) | 562, 1179 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 27, 309 |
| SmlI | CTYRAG | 3 cut(s) | 524, 562, 1179 |
| SmoI | CTYRAG | 3 cut(s) | 524, 562, 1179 |
| SsiI | CCGC | 2 cut(s) | 702, 1834 |
| SspI | AATATT | 1 cut(s) | 116 |
| SspMI | CTAG | 3 cut(s) | 578, 635, 875 |
| SstI | GAGCTC | 2 cut(s) | 1074, 1681 |
| StyD4I | CCNGG | 1 cut(s) | 617 |
| StyI | CCWWGG | 3 cut(s) | 209, 373, 1583 |
| TaaI | ACNGT | 5 cut(s) | 311, 631, 1043, 1126, 1735 |
| TaiI | ACGT | 1 cut(s) | 997 |
| TaqI | TCGA | 3 cut(s) | 563, 886, 1180 |
| TaqII | GACCGA | 1 cut(s) | 992 |
| TatI | WGTACW | 2 cut(s) | 647, 1536 |
| TfiI | GAWTC | 6 cut(s) | 590, 674, 1058, 1334, 1340, 1619 |
| TscAI | CASTG | 5 cut(s) | 636, 1122, 1131, 1147, 1696 |
| TseFI | GTSAC | 1 cut(s) | 1113 |
| TseI | GCWGC | 3 cut(s) | 585, 1013, 1831 |
| Tsp45I | GTSAC | 1 cut(s) | 1113 |
| TspDTI | ATGAA | 7 cut(s) | 210, 498, 687, 761, 1178, 1332, 1434 |
| TspGWI | ACGGA | 1 cut(s) | 1252 |
| TspRI | CASTG | 5 cut(s) | 636, 1122, 1131, 1147, 1696 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1126 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 178 |
| Vha464I | CTTAAG | 1 cut(s) | 524 |
| XapI | RAATTY | 6 cut(s) | 128, 327, 826, 1422, 1447, 1703 |
| XceI | RCATGY | 2 cut(s) | 26, 30 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 750 |
| XhoI | CTCGAG | 2 cut(s) | 562, 1179 |
| XmiI | GTMKAC | 1 cut(s) | 913 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1433 |
| XspI | CTAG | 3 cut(s) | 578, 635, 875 |
| Zsp2I | ATGCAT | 1 cut(s) | 28 |