FvH4_4g02642

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000094
Physical Location & Seq
Forward (+)
2425046 .. 2429606
4561 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g02642.t1

Sequence Viewer

Length: 4278 bp
ATGCCTCCTAGGCGCCCACGGCAACCCCGTGTGGCTACTTCGACCAATGCCGATGAAGGCAATGAGGTCAGGGGACTGGTTGATGATATTGGGAATATGCTGAGGGATGTCATGGATCGAGCTCCACAACCTCATTCTGTGAAAAGGAGAGAAGTTTTTGAAGCTGGAGCCAGAGAGTTCAAGGGTGCCAAGGGACCCCTTGATGCTTATACTTGGGTGGACAAAATGGAAAAAGCTTTTGTGAGCACAGAGTTGCCAGATGAAAAGAAGGTGAAGATGGCAGTAACTTTCCTGGATGATTCAGCACATCACTGGTGGTCGCTTGCCAGCAGGAATGTTGAGGATGCGCTGAATATGTCATGGGAACAGTTTAAGACTCTTTTCTTGGGGCAATATTTCAGTCCTGCCCATCGCAGCAGAATTCAGTCAGATTTCCTGAATATAAAGAAGAGGGATGATGAAGGCGTTATAGAGTTCCAAGAGAGATTCACTTCCCTGAGCTATCATGTGTCGTATTTGGTTCCTGATGAGAGGACCAGGATGGAGATGTTCATTGGGGCACTTGGTGGAGTGTTTGCGGATAAGATGACGGGTGTTTATTACCCGACTTTCTCTGACGCGGTTCATGCAGCCATGGCCATTGAGTCTATGGGGATTTATAGTGCTCGGCCACGAGATTTCGGTGGTCCCAGTCAGGGTCCATCCAAGAAAGCTGTTTCATCATCCGCTTCAGGTTCATCAGCTGGTAGTGGCAGTAGTGGGGGATCAGGTTCTCCTTCTCGGTTCAGGGTTAGAGGACGTCACAGGAGACCTGCTAGGAGCCAGTTGGGTAGGCAGCAGTCCGGGCAGTATAGTTCGGGTGGCAGTTTCCATGGTGGGACATCTGGTAGTCGGACTTTTTCTTATGGGCAGCAGCAGCAGTTTTCTGGGTGCTTTGAGTGTGGTAGTCATGACCACTTCAGGAGAGATTGCCCATTGTTGGCTCAGAGGACCACTCCCACCCCTACTCAGCCTGTTGGTCAGTCTTCAGCTGGAGGCTCTAGTAGTGGTGCCCAGGGCAGTGCTTCGGTCAGAGGTGGCTCTCAGCGGGGAAGTGGTCAGCGTGGACGTCCCATGACTCGCGCTAGACTACATGCTATGACCCAGCAGGAGGGTCGTACTTCTCCAGATGTTATCATTGGTACGTTATTGATTTTTGGTCAGCCTGCTTTTACTTTAATTGATCCCGGAGCCACGCATTCATTCATGTCTAGTAGATTTGCCCTCCATGCCAATGTGCCATCCTCGTCTCTTCTAGGCGAGTGGCATGTGTCTCTACCCTCTGATATAGTGGAGTTTGATGTGATCTTGGGAATGGACTTTTTGGAAGCATATAGAGCTATGGTAGATTGTTTTCAAAAAATAGTGGTGCTTCGAAGTCCAGGTGGTTTTGAGGTTGCTTTCCAAGGGGAAAGAGATGTTATTTTTAGTTGCATAATTTCCGCTATTGCTGCAAGGAAATTGTTGAGTAAAGGCTGTCAGGCATTTCTAGCTCATGTGGATACAAGTATGGGAGTTGTGGGATTAGAAGATATTCCTGTAGTAAGAGAGTTTCCGGATGTTTTTCCTGAGGAGTTGCCTGGTTTGCCTCCTGCAAGGGAGATAGACTTCTCTATAGAATTACTTCCCGGAACTATGCCGATCTCTCAGGCGCCTTACAGAATGGCTCCAGCGGAATTGAAAGAGTTGAAGACTCAATTGCAGGAGTTGGTAGATAAGGGTTTTATTCGGCCTAGCATGTCTCCATGGGGTGCTCCAGTGTTGTTTGTTAAGAAGAAAGATGGCACCATGAGACTATGCATTGATTATAGGAAGCTGAATCAGTTACGGATCCGAGAGGGGGACATAGCCAAGACTGCTTTTCGGTCACGATATGGTCATTATGAATTTGTGGTGATGCCTTTTGGACTTACTAATGCACCCGCGGCGTTCATGGATCTCATGAATAGGGTGTTTAGTCCATATCTTGATCGTTTCGTGATCGTATTCATAGATGATATCTTGGTTTACTCAAGAAGTGAGAAGGAGCATGCCAAGCATCTAAGAATAATATTGCGGACTTTGGAAAAGTCGCAGTTATTTGCTAAGTTTAGCAAGTGTGAGTTTTGGCTTGATAAAGTGGGGTTCTTGGGTCATGTGATTTCGGCAGAAGGAGTTAGTGTGGATCCCCAAAAGGTGGAAGCAGTGTCGAATTGGAGAAGACCCACCACTGTGACGGAGATTCGTAGTTTCTTGGGATTAGCAGATCAGTGCGAGCAAAGTTTTCAAGAATTGAAGAATTGCTTGACAAGTGCTCCTGTTTTGGCACTTCCGGATGATAGTGGAGAATATGTGGTCTATTGTGATGCTTCTAGACGTGGTCTAGGTGGAGTTTTGATGCAGCATGATCGAGTTATTGCCTACGCTTCTAGGCAATTGAAGCCGCATGAGGGGAATTATCCTACTCATGATCTGGAGCTTGCAGCGGTAGTTTTTGCTCTCAAGTTGTGGAGACACTATCTTTATGGAGCCAAGTGTCAGATATTTACCGATCACAAGAGTCTTCGGTATGTGTTCACTCAAAGAGAGCTTAATATGAGACAGAGAAGATGGATGGAGTTAATCAGTGATTATGATTGCACTATTGAGTATCATCCTGGAAAAGCTAATGTGGTAGCTGATGCTCTTAATCGAAATCCCTCCATATCTTTATCTCATATAAAGATGGTTCGTGTTCCGCTTCTTTGTGAGTTGCGAGCTACAGGAGTGGATTTGTCAGTGGATAAGGTTGGTGCTTTGATAGCCAGTTTTCATGTGAGGCCAGTTTTGGTTGATAGAGTGCGGGAAGCACAGTATAATGATTCTTCCATAGTTCGATTGATGGAAAGAGCTTCGAATGGAGCCGCAGCCGATCATTTTTCAGTTAGAAGAGATGAGACTCTATTGTTTAAAAATCGGCTATGTGTTCCAAAAAGAAATGAAGAGTTGAAGAGTGAGATTATGGAGGAAGCTCATAGCTCTGCATATGCTGCTCATCCGGGTAGCACAAAAATGTATCGGATTTTGAAGGATTATTATTGGTGGCCAAACATGAAGAGGGAAATTGCAGCTTATGTGAGTAGATGTCTGATTTGTCAGCAAGTCAAGGCTGAAAGGCAGAAGCCTTCAGGATTGCTTGAGCCGTTGCCCATTCCAGTGTGGAAGTGGGACCACATCACCATGGATTTTGTTTTTAGCTTACCTCGTACCCGTGATGGGCATGACGGTATTTGGGTGATTGTGGATAGACTCACCAAGTCTGCACATTTCTTGCCAGTGGGAAAGAAGTATAAATTGGATAAATTGGCGGAGCTCTATGTGAATGAAATTGTGAGGCTTCACGGTGTTCCTGAATCAATAGTGTCAGATCGGGACCCTCGATTTGCTTCTAAATTCTGGGGAGCTCTTCAGGCAGCTTTAGGTACCCAATTACTCTTCAGCACTGCTTTTCATCCTCAGACTGATGGGCAGTCCGAGAGGACTATTCAGACTTTGGAGGATATGTTGAGGGCTTGTGCCATGCAGTTCAGAGGTAGTTGGGATAAAAACCTATCTTTGATGGAATTTGCCTATAATAATAGCTATCATTCTAGCATTGGTATGGCGCTCTTTGAGGCTCTTTATGGGAGGCAGTGTCGCACGCCTTTGTGTTGGAATGAAGTAGGGGAAAAAGAGCTTTGTGGCCCAGAGTTAATCCATGTTACGAATGAGAAGATTAAAGTGGTTAGAGATAGACTCAAGACTGCTCAAAGTAGGCAGAAAAGTTATGCGGATGTCCGTAGGAGAGATCTTGAATTTCAAGTGGGTGATTGGGTGTTCCTGAAATTATCTCCTTGGAAAGGGATTGTGCGTTTCGGTAAGCGTGGGAAGCTTAGCCCGAGGTATATTGGCCCTTATGAGATTATAGAGCGAGTTGGCTCACTTGCTTATCGATTAGCCTTGCCTCCAGAGTTAGCTAGGATACATAAGGTGTTTCATGTATCCATGCTTCGCAAGTATATTGCCGATCCTTCTCATGTGCTGCAAGAGCAACCGATCAGTTTGACGAGAGATTTGACCTATGAAGAGGAACCGGTTCAGATTCTCGACCGAAAGGATCAAGTGCTTAGGAACAAGACAATCCCGCTTGTCAAGGTGCTGTGGAGAAGTCATCAAGTAGAAGAAGCTACTTGGGAATCAGAGGAGCAAATGATGCAGCAGTATCTGTATCTCTTCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve zf-CCHC

Protein Analysis

1426

Amino Acids

161.13

Weight (kDa)

9.0

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 92 - 187 1.2e-14 Retrotransposon gag protein
RVP_2 PF08284 372 - 440 2.3e-12 Retroviral aspartyl protease
RVT_1 PF00078 634 - 727 1.5e-20 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 762 - 854 9.6e-31 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 786 - 884 1.2e-32 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 999 - 1056 4e-20 Integrase zinc binding domain
SH3_Tf2-1 PF24626 1288 - 1352 5.7e-27 Tf2-1-like, SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000094)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42190
fragaria_vesca FvH4_1g22371 FvH4_1g23261 FvH4_1g27933 FvH4_1g27934 FvH4_1g29231 FvH4_2g02781 FvH4_2g08031 FvH4_2g08581 FvH4_3g06961 FvH4_3g06962 FvH4_3g13531 FvH4_3g14391 FvH4_3g14392 FvH4_3g18622 FvH4_3g18964 FvH4_3g26314 FvH4_3g28781 FvH4_3g30771 FvH4_3g30772 FvH4_3g31752 FvH4_3g32803 FvH4_3g35751 FvH4_3g35752 FvH4_4g02151 FvH4_4g02642 FvH4_4g05523 FvH4_4g05524 FvH4_4g06574 FvH4_4g06722 FvH4_4g08644 FvH4_4g08645 FvH4_4g08646 FvH4_4g08851 FvH4_4g08971 FvH4_4g09081 FvH4_4g09243 FvH4_4g09244 FvH4_4g09283 FvH4_4g10021 FvH4_4g10032 FvH4_4g10033 FvH4_4g10182 FvH4_4g10361 FvH4_4g12502 FvH4_4g12503 FvH4_4g13341 FvH4_4g13942 FvH4_4g13943 FvH4_4g13944 FvH4_4g14432 FvH4_4g14822 FvH4_4g14823 FvH4_4g15291 FvH4_4g15292 FvH4_4g15293 FvH4_4g15471 FvH4_4g15583 FvH4_4g15584 FvH4_4g15585 FvH4_4g15586 FvH4_4g15587 FvH4_4g15591 FvH4_4g15592 FvH4_4g15593 FvH4_4g16179 FvH4_4g18131 FvH4_4g21031 FvH4_4g22021 FvH4_4g22021 FvH4_4g22042 FvH4_5g03402 FvH4_5g07411 FvH4_5g13881 FvH4_5g15792 FvH4_5g24682 FvH4_5g24911 FvH4_5g25322 FvH4_5g25641 FvH4_5g36692 FvH4_5g38072 FvH4_5g38171 FvH4_6g05202 FvH4_6g08531 FvH4_6g16342 FvH4_6g21771 FvH4_6g23303 FvH4_6g23961 FvH4_6g25381 FvH4_6g27021 FvH4_6g27022 FvH4_6g30091 FvH4_6g30092 FvH4_7g02051 FvH4_7g06031 FvH4_7g07031 FvH4_7g07581 FvH4_7g08381 FvH4_7g11551 FvH4_7g19711 FvH4_7g25621 FvH4_7g29722
malus_domestica MD06G1134500.v1.1 MD09G1067600.v1.1 MD13G1145500.v1.1 MD15G1166500.v1.1 MD16G1270000.v1.1
prunus_persica Prupe.7G030900_v2.0.a1
pyrus_communis pycom01g00710 pycom01g03760 pycom03g10770 pycom04g02180 pycom05g08050 pycom06g04140 pycom06g11110 pycom07g07550 pycom07g21570 pycom08g17240 pycom09g13900 pycom09g19570 pycom10g22300 pycom11g03210 pycom11g13750 pycom11g24890 pycom12561g00270 pycom12668g00020 pycom12g11280 pycom12g20720 pycom12g20730 pycom13g21410 pycom13g21660 pycom14g06010 pycom14g06370 pycom14g12800 pycom16g21440 pycom16g22530 pycom17g02030 pycom420g00170 pycom50g00020 pycom5153684g00110 pycom520g00860 pycom581g00060
rosa_chinensis RchiOBHm_Chr0c08g0498091 RchiOBHm_Chr0c08g0498101 RchiOBHm_Chr1g0326991 RchiOBHm_Chr2g0084311 RchiOBHm_Chr2g0090701 RchiOBHm_Chr2g0098591 RchiOBHm_Chr2g0109491 RchiOBHm_Chr2g0109501 RchiOBHm_Chr2g0130801 RchiOBHm_Chr2g0145931 RchiOBHm_Chr2g0168091 RchiOBHm_Chr3g0457081 RchiOBHm_Chr3g0457091 RchiOBHm_Chr3g0467781 RchiOBHm_Chr3g0467791 RchiOBHm_Chr3g0493771 RchiOBHm_Chr3g0494051 RchiOBHm_Chr3g0496881 RchiOBHm_Chr4g0401391 RchiOBHm_Chr4g0414861 RchiOBHm_Chr4g0424081 RchiOBHm_Chr5g0009611 RchiOBHm_Chr5g0049881 RchiOBHm_Chr5g0050351 RchiOBHm_Chr5g0053791 RchiOBHm_Chr5g0078031 RchiOBHm_Chr6g0256481 RchiOBHm_Chr6g0271231 RchiOBHm_Chr6g0286381 RchiOBHm_Chr7g0197101 RchiOBHm_Chr7g0221891 RchiOBHm_Chr7g0237801 RchiOBHm_Chr7g0237821
rosa_multiflora Rmu_sc0000509.1_g000034 Rmu_sc0004751.1_g000014 Rmu_sc0008546.1_g000002 Rmu_sc0009320.1_g000003 Rmu_sc0010661.1_g000006 Rmu_sc0029652.1_g000002
rosa_roxburghii Rroxscaffold_10G00433150 Rroxscaffold_15G00447630 Rroxscaffold_26G00447790 Rroxscaffold_29G00441810 Rroxscaffold_32G00442860 Rroxscaffold_33G00439800 Rroxscaffold_34G00443230 Rroxscaffold_3G00220560 Rroxscaffold_3G00227370 Rroxscaffold_3G00239080 Rroxscaffold_3G00239090 Rroxscaffold_3G00273190 Rroxscaffold_4G00323530 Rroxscaffold_4G00326840 Rroxscaffold_57G00443110 Rroxscaffold_5G00338590 Rroxscaffold_5G00347530 Rroxscaffold_68G00446990 Rroxscaffold_76G00448570 Rroxscaffold_90G00448860
rosa_rugosa Rorug06G0233600
rosa_samantha Rh3CG302700 Rh6DG286700
rosa_wichuraiana Rw0G002280 Rw0G004080 Rw0G011830 Rw1G003690 Rw1G008800 Rw1G009130 Rw1G009140 Rw1G009150 Rw1G009160 Rw1G009410 Rw1G009580 Rw1G012770 Rw1G015850 Rw1G026860 Rw2G003780 Rw2G006480 Rw2G016520 Rw2G016960 Rw2G018170 Rw2G025180 Rw2G025510 Rw2G025610 Rw2G025750 Rw2G025920 Rw2G029950 Rw2G046360 Rw2G046370 Rw2G046380 Rw3G009070 Rw3G025820 Rw3G027340 Rw3G027900 Rw3G028150 Rw3G029060 Rw4G002440 Rw4G005960 Rw4G007520 Rw4G011970 Rw4G011980 Rw4G012190 Rw4G012530 Rw4G018490 Rw4G026380 Rw5G003970 Rw5G030920 Rw5G031780 Rw5G031900 Rw5G033030 Rw5G033040 Rw5G036330 Rw5G039730 Rw5G048110 Rw6G006670 Rw6G007910 Rw6G009720 Rw6G010990 Rw6G012930 Rw6G012940 Rw6G013420 Rw6G014690 Rw6G015290 Rw6G015790 Rw6G015920 Rw6G036510 Rw7G016650 Rw7G023140 Rw7G028650 Rw7G036450 Rw7G036460 Rw7G036910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 802, 1111
Acc36I ACCTGC 1 cut(s) 820
Acc65I GGTACC 1 cut(s) 3479
AccB1I GGYRCC 6 cut(s) 12, 185, 1049, 1690, 1823, 3479
AccB7I CCANNNNNTGG 1 cut(s) 2215
AccII CGCG 3 cut(s) 620, 1122, 1964
AccIII TCCGGA 2 cut(s) 1594, 2350
AcoI YGGCCR 3 cut(s) 636, 668, 3101
AcsI RAATTY 5 cut(s) 420, 1925, 3449, 3620, 3851
AcuI CTGAAG 6 cut(s) 714, 943, 1011, 3168, 3449, 3478
AcyI GRCGYC 4 cut(s) 13, 799, 1108, 1691
AdeI CACNNNGTG 1 cut(s) 566
AfaI GTAC 4 cut(s) 1159, 1183, 3265, 3481
AfiI CCNNNNNNNGG 8 cut(s) 695, 979, 1150, 1879, 2215, 2468, 3273, 3896
AgeI ACCGGT 1 cut(s) 4129
AjiI CACGTC 1 cut(s) 2396
AjnI CCWGG 6 cut(s) 291, 536, 1053, 1420, 1618, 2674
AjuI GAANNNNNNNTTGG 2 cut(s) 3335, 3367
AleI CACNNNNGTG 1 cut(s) 2249
Alw21I GWGCWC 7 cut(s) 124, 248, 667, 1795, 2335, 3372, 3463
Alw26I GTCTC 8 cut(s) 802, 1293, 1317, 1785, 1825, 2524, 2611, 2948
AlwNI CAGNNNCTG 1 cut(s) 4261
Ama87I CYCGRG 1 cut(s) 3934
Aor13HI TCCGGA 2 cut(s) 1594, 2350
AoxI GGCC 7 cut(s) 636, 668, 1769, 2837, 3101, 3739, 3946
ApoI RAATTY 5 cut(s) 420, 1925, 3449, 3620, 3851
ArsI GACNNNNNNTTYG 4 cut(s) 3422, 3454, 4093, 4125
AsiGI ACCGGT 1 cut(s) 4129
Asp700I GAANNNNTTC 3 cut(s) 1572, 1662, 4131
Asp718I GGTACC 1 cut(s) 3479
AspA2I CCTAGG 1 cut(s) 8
AspLEI GCGC 5 cut(s) 15, 349, 1124, 1693, 3664
AspS9I GGNCC 9 cut(s) 194, 534, 686, 698, 990, 3226, 3430, 3740, 3947
AsuC2I CCSGG 4 cut(s) 844, 1227, 1668, 3057
AsuHPI GGTGA 6 cut(s) 283, 1945, 3226, 3301, 3304, 3875
AsuII TTCGAA 2 cut(s) 1414, 2912
AvaI CYCGRG 1 cut(s) 3934
AvaII GGWCC 7 cut(s) 194, 534, 686, 698, 990, 3226, 3430
AvrII CCTAGG 1 cut(s) 8
AxyI CCTNAGG 1 cut(s) 1608
BaeGI GKGCMC 2 cut(s) 562, 1054
BalI TGGCCA 2 cut(s) 638, 3103
BamHI GGATCC 2 cut(s) 1869, 2203
BanI GGYRCC 6 cut(s) 12, 185, 1049, 1690, 1823, 3479
BanII GRGCYC 3 cut(s) 124, 3372, 3463
BarI GAAGNNNNNNTAC 2 cut(s) 4029, 4061
BauI CACGAG 1 cut(s) 672
BbsI GAAGAC 4 cut(s) 1017, 1736, 2245, 2573
Bbv12I GWGCWC 7 cut(s) 124, 248, 667, 1795, 2335, 3372, 3463
BbvCI CCTCAGC 1 cut(s) 101
BceAI ACGGC 2 cut(s) 35, 3184
BcgI CGANNNNNNTGC 4 cut(s) 1136, 1170, 2408, 2442
BciT130I CCWGG 6 cut(s) 293, 538, 1055, 1422, 1620, 2676
BciVI GTATCC 3 cut(s) 1534, 4011, 4048
BcnI CCSGG 4 cut(s) 844, 1227, 1668, 3057
BcoDI GTCTC 8 cut(s) 802, 1293, 1317, 1785, 1825, 2524, 2611, 2948
BfmI CTRYAG 3 cut(s) 1578, 1653, 2778
BfoI RGCGCY 3 cut(s) 16, 1694, 3665
BfuAI ACCTGC 1 cut(s) 820
BfuI GTATCC 3 cut(s) 1534, 4011, 4048
BglI GCCNNNNNGGC 1 cut(s) 10
BglII AGATCT 1 cut(s) 3844
BlnI CCTAGG 1 cut(s) 8
BlpI GCTNAGC 1 cut(s) 3929
Bme18I GGWCC 7 cut(s) 194, 534, 686, 698, 990, 3226, 3430
BmeT110I CYCGRG 1 cut(s) 3934
BmgBI CACGTC 1 cut(s) 2396
BmgT120I GGNCC 9 cut(s) 194, 534, 686, 698, 990, 3226, 3430, 3740, 3947
BmrI ACTGGG 1 cut(s) 684
BmsI GCATC 8 cut(s) 193, 334, 1926, 2086, 2374, 2406, 2689, 4239
BmuI ACTGGG 1 cut(s) 684
BpiI GAAGAC 4 cut(s) 1017, 1736, 2245, 2573
BplI GAGNNNNNCTC 4 cut(s) 979, 1011, 3777, 3809
BpmI CTGGAG 7 cut(s) 186, 1053, 1149, 1692, 1779, 2513, 3987
Bpu10I CCTNAGC 3 cut(s) 101, 497, 4163
Bpu1102I GCTNAGC 1 cut(s) 3929
Bpu14I TTCGAA 2 cut(s) 1414, 2912
BpuEI CTTGAG 4 cut(s) 2035, 2504, 3215, 3779
BpuMI CCSGG 4 cut(s) 844, 1227, 1668, 3057
Bsa29I ATCGAT 1 cut(s) 3988
BsaHI GRCGYC 4 cut(s) 13, 799, 1108, 1691
BsaI GGTCTC 1 cut(s) 802
BsaWI WCCGGW 3 cut(s) 1594, 2350, 4129
Bsc4I CCNNNNNNNGG 8 cut(s) 695, 979, 1150, 1879, 2215, 2468, 3273, 3896
Bse118I RCCGGY 1 cut(s) 4129
Bse1I ACTGG 9 cut(s) 81, 317, 690, 823, 1796, 2823, 2840, 3212, 3332
Bse21I CCTNAGG 1 cut(s) 1608
Bse3DI GCAATG 1 cut(s) 67
BseAI TCCGGA 2 cut(s) 1594, 2350
BseBI CCWGG 6 cut(s) 293, 538, 1055, 1422, 1620, 2676
BseCI ATCGAT 1 cut(s) 3988
BseLI CCNNNNNNNGG 8 cut(s) 695, 979, 1150, 1879, 2215, 2468, 3273, 3896
BseMI GCAATG 1 cut(s) 67
BseMII CTCAG 8 cut(s) 92, 488, 998, 1022, 1097, 1599, 1700, 3527
BseNI ACTGG 9 cut(s) 81, 317, 690, 823, 1796, 2823, 2840, 3212, 3332
BseRI GAGGAG 2 cut(s) 1625, 4253
BseSI GKGCMC 2 cut(s) 562, 1054
BseYI CCCAGC 1 cut(s) 1143
BsgI GTGCAG 1 cut(s) 3303
Bsh1236I CGCG 3 cut(s) 620, 1122, 1964
Bsh1285I CGRYCG 1 cut(s) 4147
BshFI GGCC 7 cut(s) 638, 670, 1771, 2839, 3103, 3741, 3948
BshNI GGYRCC 6 cut(s) 12, 185, 1049, 1690, 1823, 3479
BshTI ACCGGT 1 cut(s) 4129
BshVI ATCGAT 1 cut(s) 3988
BsiEI CGRYCG 1 cut(s) 4147
BsiHKAI GWGCWC 7 cut(s) 124, 248, 667, 1795, 2335, 3372, 3463
BsiHKCI CYCGRG 1 cut(s) 3934
BsiSI CCGG 7 cut(s) 843, 1227, 1595, 1668, 2351, 3056, 4130
BslFI GGGAC 8 cut(s) 87, 207, 672, 892, 1095, 1895, 3239, 3443
BslI CCNNNNNNNGG 8 cut(s) 695, 979, 1150, 1879, 2215, 2468, 3273, 3896
BsmAI GTCTC 8 cut(s) 802, 1293, 1317, 1785, 1825, 2524, 2611, 2948
BsmBI CGTCTC 1 cut(s) 1293
BsmFI GGGAC 8 cut(s) 87, 207, 672, 892, 1095, 1895, 3239, 3443
BsmI GAATGC 1 cut(s) 1237
BsnI GGCC 7 cut(s) 638, 670, 1771, 2839, 3103, 3741, 3948
Bso31I GGTCTC 1 cut(s) 802
BsoBI CYCGRG 1 cut(s) 3934
Bsp119I TTCGAA 2 cut(s) 1414, 2912
Bsp1286I GDGCHC 9 cut(s) 124, 248, 562, 667, 1054, 1795, 2335, 3372, 3463
Bsp13I TCCGGA 2 cut(s) 1594, 2350
Bsp1720I GCTNAGC 1 cut(s) 3929
Bsp19I CCATGG 4 cut(s) 633, 871, 1784, 3237
BspANI GGCC 7 cut(s) 638, 670, 1771, 2839, 3103, 3741, 3948
BspCNI CTCAG 8 cut(s) 93, 489, 997, 1021, 1096, 1600, 1699, 3526
BspDI ATCGAT 1 cut(s) 3988
BspEI TCCGGA 2 cut(s) 1594, 2350
BspFNI CGCG 3 cut(s) 620, 1122, 1964
BspHI TCATGA 3 cut(s) 949, 1980, 2485
BspMI ACCTGC 1 cut(s) 820
BspQI GCTCTTC 1 cut(s) 3468
BspT104I TTCGAA 2 cut(s) 1414, 2912
BspT107I GGYRCC 6 cut(s) 12, 185, 1049, 1690, 1823, 3479
BspTNI GGTCTC 1 cut(s) 802
BsrDI GCAATG 1 cut(s) 67
BsrFI RCCGGY 1 cut(s) 4129
BsrI ACTGG 9 cut(s) 81, 317, 690, 823, 1796, 2823, 2840, 3212, 3332
BssAI RCCGGY 1 cut(s) 4129
BssNI GRCGYC 4 cut(s) 13, 799, 1108, 1691
BssSI CACGAG 1 cut(s) 672
BssT1I CCWWGG 8 cut(s) 8, 189, 633, 871, 1444, 1784, 3237, 3890
Bst2BI CACGAG 1 cut(s) 672
Bst2UI CCWGG 6 cut(s) 293, 538, 1055, 1422, 1620, 2676
Bst4CI ACNGT 5 cut(s) 369, 2251, 2871, 3284, 3401
BstACI GRCGYC 4 cut(s) 13, 799, 1108, 1691
BstAPI GCANNNNNTGC 2 cut(s) 3047, 4249
BstBI TTCGAA 2 cut(s) 1414, 2912
BstC8I GCNNGC 8 cut(s) 324, 328, 1206, 2070, 2294, 2499, 2775, 3698
BstDSI CCRYGG 6 cut(s) 17, 633, 871, 1784, 1962, 3237
BstENI CCTNNNNNAGG 1 cut(s) 3894
BstFNI CGCG 3 cut(s) 620, 1122, 1964
BstH2I RGCGCY 3 cut(s) 16, 1694, 3665
BstHHI GCGC 5 cut(s) 15, 349, 1124, 1693, 3664
BstMAI GTCTC 8 cut(s) 802, 1293, 1317, 1785, 1825, 2524, 2611, 2948
BstMCI CGRYCG 1 cut(s) 4147
BstNI CCWGG 6 cut(s) 293, 538, 1055, 1422, 1620, 2676
BstNSI RCATGY 4 cut(s) 1136, 1310, 1780, 2072
BstSFI CTRYAG 3 cut(s) 1578, 1653, 2778
BstSLI GKGCMC 2 cut(s) 562, 1054
BstUI CGCG 3 cut(s) 620, 1122, 1964
BstV2I GAAGAC 4 cut(s) 1017, 1736, 2245, 2573
BstX2I RGATCY 4 cut(s) 1869, 1975, 2203, 3844
BstYI RGATCY 4 cut(s) 1869, 1975, 2203, 3844
Bsu15I ATCGAT 1 cut(s) 3988
Bsu36I CCTNAGG 1 cut(s) 1608
BsuI GTATCC 3 cut(s) 1534, 4011, 4048
BsuRI GGCC 7 cut(s) 638, 670, 1771, 2839, 3103, 3741, 3948
BsuTUI ATCGAT 1 cut(s) 3988
BtgI CCRYGG 6 cut(s) 17, 633, 871, 1784, 1962, 3237
BtgZI GCGATG 1 cut(s) 395
BtrI CACGTC 1 cut(s) 2396
BtsI GCAGTG 4 cut(s) 1066, 2229, 3498, 3695
BveI ACCTGC 1 cut(s) 820
Cac8I GCNNGC 8 cut(s) 324, 328, 1206, 2070, 2294, 2499, 2775, 3698
CaiI CAGNNNCTG 1 cut(s) 4261
CciI TCATGA 3 cut(s) 949, 1980, 2485
CfoI GCGC 5 cut(s) 15, 349, 1124, 1693, 3664
Cfr10I RCCGGY 1 cut(s) 4129
Cfr13I GGNCC 9 cut(s) 194, 534, 686, 698, 990, 3226, 3430, 3740, 3947
Cfr42I CCGCGG 1 cut(s) 1965
ClaI ATCGAT 1 cut(s) 3988
CseI GACGC 1 cut(s) 626
Csp6I GTAC 4 cut(s) 1158, 1182, 3264, 3480
CspAI ACCGGT 1 cut(s) 4129
CviQI GTAC 4 cut(s) 1158, 1182, 3264, 3480
DinI GGCGCC 2 cut(s) 14, 1692
DraI TTTAAA 1 cut(s) 2968
DraIII CACNNNGTG 1 cut(s) 566
EaeI YGGCCR 3 cut(s) 636, 668, 3101
EciI GGCGGA 1 cut(s) 3380
Ecl136II GAGCTC 3 cut(s) 122, 3370, 3461
Eco130I CCWWGG 8 cut(s) 8, 189, 633, 871, 1444, 1784, 3237, 3890
Eco24I GRGCYC 3 cut(s) 124, 3372, 3463
Eco31I GGTCTC 1 cut(s) 802
Eco32I GATATC 1 cut(s) 2038
Eco47I GGWCC 7 cut(s) 194, 534, 686, 698, 990, 3226, 3430
Eco53kI GAGCTC 3 cut(s) 122, 3370, 3461
Eco57I CTGAAG 6 cut(s) 714, 943, 1011, 3168, 3449, 3478
Eco81I CCTNAGG 1 cut(s) 1608
Eco88I CYCGRG 1 cut(s) 3934
EcoICRI GAGCTC 3 cut(s) 122, 3370, 3461
EcoNI CCTNNNNNAGG 1 cut(s) 3894
EcoO109I RGGNCCY 2 cut(s) 194, 3430
EcoRI GAATTC 1 cut(s) 420
EcoRII CCWGG 6 cut(s) 291, 536, 1053, 1420, 1618, 2674
EcoRV GATATC 1 cut(s) 2038
EcoT14I CCWWGG 8 cut(s) 8, 189, 633, 871, 1444, 1784, 3237, 3890
EcoT22I ATGCAT 1 cut(s) 1841
EcoT38I GRGCYC 3 cut(s) 124, 3372, 3463
EgeI GGCGCC 2 cut(s) 14, 1692
EheI GGCGCC 2 cut(s) 14, 1692
ErhI CCWWGG 8 cut(s) 8, 189, 633, 871, 1444, 1784, 3237, 3890
Esp3I CGTCTC 1 cut(s) 1293
FalI AAGNNNNNCTT 2 cut(s) 2306, 2338
FaqI GGGAC 8 cut(s) 87, 207, 672, 892, 1095, 1895, 3239, 3443
FauI CCCGC 4 cut(s) 1080, 1969, 2853, 4188
FauNDI CATATG 1 cut(s) 3043
FriOI GRGCYC 3 cut(s) 124, 3372, 3463
GlaI GCGC 5 cut(s) 14, 348, 1123, 1692, 3663
GsaI CCCAGC 1 cut(s) 1147
GsuI CTGGAG 7 cut(s) 186, 1053, 1149, 1692, 1779, 2513, 3987
HaeII RGCGCY 3 cut(s) 16, 1694, 3665
HaeIII GGCC 7 cut(s) 638, 670, 1771, 2839, 3103, 3741, 3948
HapII CCGG 7 cut(s) 843, 1227, 1595, 1668, 2351, 3056, 4130
HgaI GACGC 1 cut(s) 626
HhaI GCGC 5 cut(s) 15, 349, 1124, 1693, 3664
Hin1I GRCGYC 4 cut(s) 13, 799, 1108, 1691
Hin6I GCGC 5 cut(s) 13, 347, 1122, 1691, 3662
HinP1I GCGC 5 cut(s) 13, 347, 1122, 1691, 3662
HindIII AAGCTT 2 cut(s) 234, 3926
HpaII CCGG 7 cut(s) 843, 1227, 1595, 1668, 2351, 3056, 4130
HphI GGTGA 6 cut(s) 283, 1945, 3226, 3301, 3304, 3875
Hpy166II GTNNAC 4 cut(s) 220, 1106, 2047, 2595
Hpy8I GTNNAC 4 cut(s) 220, 1106, 2047, 2595
HpyAV CCTTC 9 cut(s) 50, 262, 455, 786, 2056, 2183, 3079, 3192, 4077
HpyCH4III ACNGT 5 cut(s) 369, 2251, 2871, 3284, 3401
HpyCH4IV ACGT 4 cut(s) 799, 1108, 1184, 2395
HpySE526I ACGT 4 cut(s) 799, 1108, 1184, 2395
Hsp92I GRCGYC 4 cut(s) 13, 799, 1108, 1691
HspAI GCGC 5 cut(s) 13, 347, 1122, 1691, 3662
KasI GGCGCC 2 cut(s) 12, 1690
KflI GGGWCCC 2 cut(s) 194, 3430
Kpn2I TCCGGA 2 cut(s) 1594, 2350
KpnI GGTACC 1 cut(s) 3483
KspI CCGCGG 1 cut(s) 1965
LguI GCTCTTC 1 cut(s) 3468
LweI GCATC 8 cut(s) 193, 334, 1926, 2086, 2374, 2406, 2689, 4239
MaeII ACGT 4 cut(s) 799, 1108, 1184, 2395
MaeIII GTNAC 6 cut(s) 283, 800, 1863, 1905, 2251, 3757
MfeI CAATTG 2 cut(s) 1736, 2453
MflI RGATCY 4 cut(s) 1869, 1975, 2203, 3844
MhlI GDGCHC 9 cut(s) 124, 248, 562, 667, 1054, 1795, 2335, 3372, 3463
MlsI TGGCCA 2 cut(s) 638, 3103
MluNI TGGCCA 2 cut(s) 638, 3103
Mly113I GGCGCC 2 cut(s) 13, 1691
MlyI GAGTC 8 cut(s) 370, 653, 1111, 1726, 2587, 2950, 3300, 3786
MmeI TCCRAC 2 cut(s) 872, 3689
Mox20I TGGCCA 2 cut(s) 638, 3103
Mph1103I ATGCAT 1 cut(s) 1841
MroI TCCGGA 2 cut(s) 1594, 2350
MroXI GAANNNNTTC 3 cut(s) 1572, 1662, 4131
MscI TGGCCA 2 cut(s) 638, 3103
MseI TTAA 9 cut(s) 372, 1217, 1809, 2610, 2639, 2706, 2967, 3749, 3774
MslI CAYNNNNRTG 6 cut(s) 1272, 2249, 3068, 3212, 3236, 3656
Msp20I TGGCCA 2 cut(s) 638, 3103
MspA1I CMGCKG 6 cut(s) 743, 1031, 1087, 1712, 1964, 2504
MspI CCGG 7 cut(s) 843, 1227, 1595, 1668, 2351, 3056, 4130
MunI CAATTG 2 cut(s) 1736, 2453
Mva1269I GAATGC 1 cut(s) 1237
MvaI CCWGG 6 cut(s) 293, 538, 1055, 1422, 1620, 2676
MvnI CGCG 3 cut(s) 620, 1122, 1964
NarI GGCGCC 2 cut(s) 13, 1691
NciI CCSGG 4 cut(s) 844, 1227, 1668, 3057
NcoI CCATGG 4 cut(s) 633, 871, 1784, 3237
NdeI CATATG 1 cut(s) 3043
NmeAIII GCCGAG 1 cut(s) 646
NmuCI GTSAC 3 cut(s) 800, 1905, 2251
NsiI ATGCAT 1 cut(s) 1841
NspI RCATGY 4 cut(s) 1136, 1310, 1780, 2072
NspV TTCGAA 2 cut(s) 1414, 2912
OliI CACNNNNGTG 1 cut(s) 2249
PaeI GCATGC 1 cut(s) 2072
PagI TCATGA 3 cut(s) 949, 1980, 2485
PasI CCCWGGG 1 cut(s) 1054
PciSI GCTCTTC 1 cut(s) 3468
PcsI WCGNNNNNNNCGW 2 cut(s) 25, 3433
PctI GAATGC 1 cut(s) 1237
PdmI GAANNNNTTC 3 cut(s) 1572, 1662, 4131
PfeI GAWTC 8 cut(s) 299, 486, 1858, 2260, 2879, 3410, 4138, 4232
PflFI GACNNNGTC 1 cut(s) 2397
PflMI CCANNNNNTGG 1 cut(s) 2215
PfoI TCCNGGA 4 cut(s) 291, 1225, 1666, 2674
PinAI ACCGGT 1 cut(s) 4129
PleI GAGTC 8 cut(s) 370, 652, 1111, 1726, 2586, 2950, 3300, 3786
PluTI GGCGCC 2 cut(s) 16, 1694
PpsI GAGTC 8 cut(s) 370, 652, 1111, 1726, 2586, 2950, 3300, 3786
PpuMI RGGWCCY 2 cut(s) 194, 3430
Psp124BI GAGCTC 3 cut(s) 124, 3372, 3463
Psp5II RGGWCCY 2 cut(s) 194, 3430
Psp6I CCWGG 6 cut(s) 291, 536, 1053, 1420, 1618, 2674
PspFI CCCAGC 1 cut(s) 1143
PspGI CCWGG 6 cut(s) 291, 536, 1053, 1420, 1618, 2674
PspPI GGNCC 9 cut(s) 194, 534, 686, 698, 990, 3226, 3430, 3740, 3947
PspPPI RGGWCCY 2 cut(s) 194, 3430
PstNI CAGNNNCTG 1 cut(s) 4261
PsuI RGATCY 4 cut(s) 1869, 1975, 2203, 3844
PsyI GACNNNGTC 1 cut(s) 2397
PvuII CAGCTG 2 cut(s) 743, 1031
RsaI GTAC 4 cut(s) 1159, 1183, 3265, 3481
RsaNI GTAC 4 cut(s) 1158, 1182, 3264, 3480
RseI CAYNNNNRTG 6 cut(s) 1272, 2249, 3068, 3212, 3236, 3656
SacI GAGCTC 3 cut(s) 124, 3372, 3463
SacII CCGCGG 1 cut(s) 1965
SapI GCTCTTC 1 cut(s) 3468
SaqAI TTAA 9 cut(s) 372, 1217, 1809, 2610, 2639, 2706, 2967, 3749, 3774
Sau96I GGNCC 9 cut(s) 194, 534, 686, 698, 990, 3226, 3430, 3740, 3947
SchI GAGTC 8 cut(s) 370, 653, 1111, 1726, 2587, 2950, 3300, 3786
SduI GDGCHC 9 cut(s) 124, 248, 562, 667, 1054, 1795, 2335, 3372, 3463
SfaNI GCATC 8 cut(s) 193, 334, 1926, 2086, 2374, 2406, 2689, 4239
SfcI CTRYAG 3 cut(s) 1578, 1653, 2778
SfoI GGCGCC 2 cut(s) 14, 1692
Sfr303I CCGCGG 1 cut(s) 1965
SfuI TTCGAA 2 cut(s) 1414, 2912
SgrBI CCGCGG 1 cut(s) 1965
SinI GGWCC 7 cut(s) 194, 534, 686, 698, 990, 3226, 3430
SmiMI CAYNNNNRTG 6 cut(s) 1272, 2249, 3068, 3212, 3236, 3656
SmlI CTYRAG 4 cut(s) 2050, 2519, 3194, 3794
SmoI CTYRAG 4 cut(s) 2050, 2519, 3194, 3794
SphI GCATGC 1 cut(s) 2072
SspDI GGCGCC 2 cut(s) 12, 1690
SspI AATATT 2 cut(s) 395, 2091
SstI GAGCTC 3 cut(s) 124, 3372, 3463
StyI CCWWGG 8 cut(s) 8, 189, 633, 871, 1444, 1784, 3237, 3890
TaaI ACNGT 5 cut(s) 369, 2251, 2871, 3284, 3401
TaiI ACGT 4 cut(s) 802, 1111, 1187, 2398
TaqII GACCGA 3 cut(s) 1057, 1893, 4161
TauI GCSGC 3 cut(s) 1967, 2464, 2924
TfiI GAWTC 8 cut(s) 299, 486, 1858, 2260, 2879, 3410, 4138, 4232
Tru1I TTAA 9 cut(s) 372, 1217, 1809, 2610, 2639, 2706, 2967, 3749, 3774
Tru9I TTAA 9 cut(s) 372, 1217, 1809, 2610, 2639, 2706, 2967, 3749, 3774
TseFI GTSAC 3 cut(s) 800, 1905, 2251
Tsp45I GTSAC 3 cut(s) 800, 1905, 2251
TspGWI ACGGA 3 cut(s) 1882, 2270, 3824
Tth111I GACNNNGTC 1 cut(s) 2397
Van91I CCANNNNNTGG 1 cut(s) 2215
VpaK11BI GGWCC 7 cut(s) 194, 534, 686, 698, 990, 3226, 3430
XagI CCTNNNNNAGG 1 cut(s) 3894
XapI RAATTY 5 cut(s) 420, 1925, 3449, 3620, 3851
XbaI TCTAGA 1 cut(s) 2390
XceI RCATGY 4 cut(s) 1136, 1310, 1780, 2072
XcmI CCANNNNNNNNNTGG 2 cut(s) 646, 3219
XmaJI CCTAGG 1 cut(s) 8
XmnI GAANNNNTTC 3 cut(s) 1572, 1662, 4131
ZraI GACGTC 2 cut(s) 800, 1109
Zsp2I ATGCAT 1 cut(s) 1841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.