Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0000094

Physical Location & Seq

Genomic Coordinates Forward (+)

27713756 .. 27716455

Gene Structure 2700 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G023140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1851 bp

ATGGCCCGTCTGCAGCGGATCCCCATCATATGCTCTTCGGTGATGGACCCTCAAGGAGGCAGAGCTAGGGGTCGAGGTAGACCCAGAGGCAGGGGTAGAGCCCGAGGTAGGGGCAGGATTCCTCCTCCTGTTGAGGAGATATTCGAGGATGAAGTTGAGGCATCAAATGTTGAGTTGCCTGTTCCACCTGTGGTGGAGGTTGCTAATGTTGTAGATCCTACTCTGAGGGAGCAGATGGAAAGGGAATTCATCTCGTTAGTCCAAGGGACTAAGAGTGTGAGGGAGTATGAAGCTGAGTTCTCAAGGTTGTATCGCTTTGTGAGGCAGATGGATGCTGAGAGCTTAGCTATGAAGTTTCAGTGGGGACTGAATGCTTCAATCCGGCGCGATGTCGCTGTTCTCGGACTGAAGACGGTGGAGCTCATTTTCGTTAAGGCTATGGCCATTGAGCAGGAGAACTTGACTCTCCAAGAACACGAATCGGCTGAGAGGGATTTCCAAAGAAAGGGAAAGGCAACTGCGGGGAGCAAGACAAGGACTAATCAGCAGGCACCAGCTAGGGCTGCAGCTGCACCTGCTAGGGTTGCACCTAATAGGCCGGTGGCGAACCCGAAGTGTTTCAATTGCAATGAGATGGGCCATGTTGCTCGAGATTGCACGAAACCTAAGAACTTGGCATGCTTCACATGTGGCCAGACGGGGCACTTCTCAAGGGATTGTACCCAGCAGGGTACCTTATCCTTATTTGATTTCTTTGCTAGGGTGTTATTTGATACGGGAGCGTCCCACTCTTTCATATCTAGTTCTGTGGTTGATATGTTAGGTTTGACTCCTAGGCCTCTTGCTAGACCTTTGTGTGTTATTTCCCCACTTGGTGTTTCCCTTGAGCTTGATATGTTTTGTGATGCTTGTCCAATTGTGATTGGTGGTAAGGAGTTTACCGCAACCTTGATTGTGTTAGCAGATCATAAGTATGATGTCATTTTGGGTATTGATTGGCTGAGGCCAAATCATGCTTTGATTGATTGCTTTGAAATGGTAGTGTCATTTCATATACCTGGACAGCCGGTGTTTCGTTATCGTGATGTATTTCAGGAAATACCAGGATTACCTCCAAGAAGGGAAGTGGACTTTGCTATCGATGTGATACCAGGTACTGCACCTCAAGGGTTCATTAGACCTAGTACTTCTCCTTGGGGTGCACCTGTGGTGTTTGCGAAGAAGAGGGATGGTTCACTAAGGTTGTGTGTGGATTATCGGCAGTTGAACAAGGTGACGATTAAGAATAGGTACCCCTTACCTAGGATTGATGACTTGTTCGACCAGCTTAAGGAAGCTGCTGTATTCTCGAAGATTGATTTGAGATCCGGATATCACCAGTTGAGGGTGAAAGATGATGATGTTCCTAAGACTGCTTTTAGGACTAGGTATGGACACTTTGAGTTTGTTGTCATGCCTTTTGGTTTAACCAATGCACCTGCAGCCTTTATGGACTTGATGAACCGTATGTTCAGTCCGTACTTGGATAAGTTTGTGGTAGTGTTCGTGGATGATATTTTGGTTTATTCCAAGACGTTGGAGGACCATGATAAGCATCTGCGGATTGTTCTACAACTTTTGAGAGAAAAGGAATTGTTTGCGAAATTCGAGAAGTGCGAATTTTGGCAAAGGGAGGTCAAGTTCCTTGGTCATGTGATTTCGAAGGATGGAATCTCTGTGGATCCTTCTAAGGTGGAAGCAGTGATGAATTGGGCCAACCCACTACAGTTACTGAGGTACGTAGTTTTCTGGGTTTGGCAGGTTATTATCGGAGATTCATTGAGGGATTTTCCAGCATTGCTTCGGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000943 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0003887 GO:0003964 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006278 GO:0006508 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0018130 GO:0019438 GO:0019538 GO:0032196 GO:0032197 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0071897 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0140096 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RVP_2 RVT_1 Retrotrans_gag rve zf-CCHC

Protein Analysis

616

Amino Acids

69.8

Weight (kDa)

8.77

Isoelectric Point (pI)

36.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Retrotrans_gag	PF03732	71 - 124	2.3e-08	Retrotransposon gag protein
zf-CCHC	PF00098	205 - 221	1.2e-07	Zinc knuckle
zf-CCHC	PF00098	226 - 242	1.1e-07	Zinc knuckle
RVP_2	PF08284	243 - 350	7.9e-30	Retroviral aspartyl protease
gag-asp_proteas	PF13975	254 - 335	7.9e-06	gag-polyprotein putative aspartyl protease
RVT_1	PF00078	407 - 566	6.1e-39	Reverse transcriptase (RNA-dependent DNA polymerase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0000094)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G42190
fragaria_vesca	FvH4_1g22371 FvH4_1g23261 FvH4_1g27933 FvH4_1g27934 FvH4_1g29231 FvH4_2g02781 FvH4_2g08031 FvH4_2g08581 FvH4_3g06961 FvH4_3g06962 FvH4_3g13531 FvH4_3g14391 FvH4_3g14392 FvH4_3g18622 FvH4_3g18964 FvH4_3g26314 FvH4_3g28781 FvH4_3g30771 FvH4_3g30772 FvH4_3g31752 FvH4_3g32803 FvH4_3g35751 FvH4_3g35752 FvH4_4g02151 FvH4_4g02642 FvH4_4g05523 FvH4_4g05524 FvH4_4g06574 FvH4_4g06722 FvH4_4g08644 FvH4_4g08645 FvH4_4g08646 FvH4_4g08851 FvH4_4g08971 FvH4_4g09081 FvH4_4g09243 FvH4_4g09244 FvH4_4g09283 FvH4_4g10021 FvH4_4g10032 FvH4_4g10033 FvH4_4g10182 FvH4_4g10361 FvH4_4g12502 FvH4_4g12503 FvH4_4g13341 FvH4_4g13942 FvH4_4g13943 FvH4_4g13944 FvH4_4g14432 FvH4_4g14822 FvH4_4g14823 FvH4_4g15291 FvH4_4g15292 FvH4_4g15293 FvH4_4g15471 FvH4_4g15583 FvH4_4g15584 FvH4_4g15585 FvH4_4g15586 FvH4_4g15587 FvH4_4g15591 FvH4_4g15592 FvH4_4g15593 FvH4_4g16179 FvH4_4g18131 FvH4_4g21031 FvH4_4g22021 FvH4_4g22021 FvH4_4g22042 FvH4_5g03402 FvH4_5g07411 FvH4_5g13881 FvH4_5g15792 FvH4_5g24682 FvH4_5g24911 FvH4_5g25322 FvH4_5g25641 FvH4_5g36692 FvH4_5g38072 FvH4_5g38171 FvH4_6g05202 FvH4_6g08531 FvH4_6g16342 FvH4_6g21771 FvH4_6g23303 FvH4_6g23961 FvH4_6g25381 FvH4_6g27021 FvH4_6g27022 FvH4_6g30091 FvH4_6g30092 FvH4_7g02051 FvH4_7g06031 FvH4_7g07031 FvH4_7g07581 FvH4_7g08381 FvH4_7g11551 FvH4_7g19711 FvH4_7g25621 FvH4_7g29722
malus_domestica	MD06G1134500.v1.1 MD09G1067600.v1.1 MD13G1145500.v1.1 MD15G1166500.v1.1 MD16G1270000.v1.1
prunus_persica	Prupe.7G030900_v2.0.a1
pyrus_communis	pycom01g00710 pycom01g03760 pycom03g10770 pycom04g02180 pycom05g08050 pycom06g04140 pycom06g11110 pycom07g07550 pycom07g21570 pycom08g17240 pycom09g13900 pycom09g19570 pycom10g22300 pycom11g03210 pycom11g13750 pycom11g24890 pycom12561g00270 pycom12668g00020 pycom12g11280 pycom12g20720 pycom12g20730 pycom13g21410 pycom13g21660 pycom14g06010 pycom14g06370 pycom14g12800 pycom16g21440 pycom16g22530 pycom17g02030 pycom420g00170 pycom50g00020 pycom5153684g00110 pycom520g00860 pycom581g00060
rosa_chinensis	RchiOBHm_Chr0c08g0498091 RchiOBHm_Chr0c08g0498101 RchiOBHm_Chr1g0326991 RchiOBHm_Chr2g0084311 RchiOBHm_Chr2g0090701 RchiOBHm_Chr2g0098591 RchiOBHm_Chr2g0109491 RchiOBHm_Chr2g0109501 RchiOBHm_Chr2g0130801 RchiOBHm_Chr2g0145931 RchiOBHm_Chr2g0168091 RchiOBHm_Chr3g0457081 RchiOBHm_Chr3g0457091 RchiOBHm_Chr3g0467781 RchiOBHm_Chr3g0467791 RchiOBHm_Chr3g0493771 RchiOBHm_Chr3g0494051 RchiOBHm_Chr3g0496881 RchiOBHm_Chr4g0401391 RchiOBHm_Chr4g0414861 RchiOBHm_Chr4g0424081 RchiOBHm_Chr5g0009611 RchiOBHm_Chr5g0049881 RchiOBHm_Chr5g0050351 RchiOBHm_Chr5g0053791 RchiOBHm_Chr5g0078031 RchiOBHm_Chr6g0256481 RchiOBHm_Chr6g0271231 RchiOBHm_Chr6g0286381 RchiOBHm_Chr7g0197101 RchiOBHm_Chr7g0221891 RchiOBHm_Chr7g0237801 RchiOBHm_Chr7g0237821
rosa_multiflora	Rmu_sc0000509.1_g000034 Rmu_sc0004751.1_g000014 Rmu_sc0008546.1_g000002 Rmu_sc0009320.1_g000003 Rmu_sc0010661.1_g000006 Rmu_sc0029652.1_g000002
rosa_roxburghii	Rroxscaffold_10G00433150 Rroxscaffold_15G00447630 Rroxscaffold_26G00447790 Rroxscaffold_29G00441810 Rroxscaffold_32G00442860 Rroxscaffold_33G00439800 Rroxscaffold_34G00443230 Rroxscaffold_3G00220560 Rroxscaffold_3G00227370 Rroxscaffold_3G00239080 Rroxscaffold_3G00239090 Rroxscaffold_3G00273190 Rroxscaffold_4G00323530 Rroxscaffold_4G00326840 Rroxscaffold_57G00443110 Rroxscaffold_5G00338590 Rroxscaffold_5G00347530 Rroxscaffold_68G00446990 Rroxscaffold_76G00448570 Rroxscaffold_90G00448860
rosa_rugosa	Rorug06G0233600
rosa_samantha	Rh3CG302700 Rh6DG286700
rosa_wichuraiana	Rw0G002280 Rw0G004080 Rw0G011830 Rw1G003690 Rw1G008800 Rw1G009130 Rw1G009140 Rw1G009150 Rw1G009160 Rw1G009410 Rw1G009580 Rw1G012770 Rw1G015850 Rw1G026860 Rw2G003780 Rw2G006480 Rw2G016520 Rw2G016960 Rw2G018170 Rw2G025180 Rw2G025510 Rw2G025610 Rw2G025750 Rw2G025920 Rw2G029950 Rw2G046360 Rw2G046370 Rw2G046380 Rw3G009070 Rw3G025820 Rw3G027340 Rw3G027900 Rw3G028150 Rw3G029060 Rw4G002440 Rw4G005960 Rw4G007520 Rw4G011970 Rw4G011980 Rw4G012190 Rw4G012530 Rw4G018490 Rw4G026380 Rw5G003970 Rw5G030920 Rw5G031780 Rw5G031900 Rw5G033030 Rw5G033040 Rw5G036330 Rw5G039730 Rw5G048110 Rw6G006670 Rw6G007910 Rw6G009720 Rw6G010990 Rw6G012930 Rw6G012940 Rw6G013420 Rw6G014690 Rw6G015290 Rw6G015790 Rw6G015920 Rw6G036510 Rw7G016650 Rw7G023140 Rw7G028650 Rw7G036450 Rw7G036460 Rw7G036910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	583, 1486
Acc36I	ACCTGC	3 cut(s)	583, 1486, 1789
Acc65I	GGTACC	2 cut(s)	731, 1290
AccB1I	GGYRCC	3 cut(s)	550, 731, 1290
AccI	GTMKAC	1 cut(s)	79
AccII	CGCG	1 cut(s)	387
AccIII	TCCGGA	1 cut(s)	1367
AciI	CCGC	4 cut(s)	16, 521, 942, 1600
AclWI	GGATC	6 cut(s)	13, 26, 209, 1359, 1715, 1728
AcoI	YGGCCR	2 cut(s)	441, 691
AcsI	RAATTY	3 cut(s)	245, 1643, 1658
AcuI	CTGAAG	1 cut(s)	428
AdeI	CACNNNGTG	1 cut(s)	875
AfaI	GTAC	7 cut(s)	721, 733, 1156, 1186, 1292, 1520, 1778
AfiI	CCNNNNNNNGG	8 cut(s)	56, 90, 108, 109, 505, 1302, 1330, 1384
AflII	CTTAAG	1 cut(s)	1328
AflIII	ACRYGT	1 cut(s)	686
AgsI	TTSAA	4 cut(s)	378, 622, 1034, 1267
AjnI	CCWGG	3 cut(s)	1057, 1102, 1150
AloI	GAACNNNNNNTCC	2 cut(s)	1664, 1696
Alw21I	GWGCWC	2 cut(s)	423, 1204
Alw44I	GTGCAC	1 cut(s)	1200
AlwI	GGATC	6 cut(s)	13, 26, 209, 1359, 1715, 1728
AlwNI	CAGNNNCTG	2 cut(s)	1157, 1771
Ama87I	CYCGRG	2 cut(s)	102, 648
Aor13HI	TCCGGA	1 cut(s)	1367
AoxI	GGCC	8 cut(s)	3, 441, 596, 637, 691, 836, 1004, 1752
ApaLI	GTGCAC	1 cut(s)	1200
ApeKI	GCWGC	6 cut(s)	13, 563, 566, 569, 1337, 1481
ApoI	RAATTY	3 cut(s)	245, 1643, 1658
Asp700I	GAANNNNTTC	1 cut(s)	617
Asp718I	GGTACC	2 cut(s)	731, 1290
AspA2I	CCTAGG	2 cut(s)	833, 1301
AspLEI	GCGC	1 cut(s)	387
AspS9I	GGNCC	5 cut(s)	4, 46, 637, 1582, 1752
AsuHPI	GGTGA	4 cut(s)	52, 1285, 1367, 1399
AsuII	TTCGAA	1 cut(s)	1700
AvaI	CYCGRG	2 cut(s)	102, 648
AvaII	GGWCC	2 cut(s)	46, 1582
AvrII	CCTAGG	2 cut(s)	833, 1301
BaeGI	GKGCMC	2 cut(s)	705, 1204
BalI	TGGCCA	2 cut(s)	443, 693
BamHI	GGATCC	2 cut(s)	18, 1720
BanI	GGYRCC	3 cut(s)	550, 731, 1290
BanII	GRGCYC	2 cut(s)	103, 423
BbsI	GAAGAC	1 cut(s)	416
Bbv12I	GWGCWC	2 cut(s)	423, 1204
BbvCI	CCTCAGC	1 cut(s)	1001
BbvI	GCAGC	6 cut(s)	25, 550, 556, 578, 1324, 1493
BccI	CCATC	7 cut(s)	32, 37, 229, 322, 628, 1223, 1700
BciT130I	CCWGG	3 cut(s)	1059, 1104, 1152
BfmI	CTRYAG	4 cut(s)	11, 564, 1479, 1763
BfrI	CTTAAG	1 cut(s)	1328
BfuAI	ACCTGC	3 cut(s)	583, 1486, 1789
BisI	GCNGC	6 cut(s)	14, 564, 567, 570, 1338, 1482
BlnI	CCTAGG	2 cut(s)	833, 1301
BlpI	GCTNAGC	1 cut(s)	343
BlsI	GCNGC	6 cut(s)	15, 565, 568, 571, 1339, 1483
BmcAI	AGTACT	1 cut(s)	1186
Bme1390I	CCNGG	3 cut(s)	1059, 1104, 1152
Bme18I	GGWCC	2 cut(s)	46, 1582
BmeT110I	CYCGRG	2 cut(s)	102, 648
BmgT120I	GGNCC	5 cut(s)	4, 46, 637, 1582, 1752
BmiI	GGNNCC	6 cut(s)	20, 48, 552, 733, 1292, 1722
BmrFI	CCNGG	3 cut(s)	1059, 1104, 1152
BmsI	GCATC	4 cut(s)	170, 322, 895, 1603
BpiI	GAAGAC	1 cut(s)	416
Bpu10I	CCTNAGC	1 cut(s)	1001
Bpu1102I	GCTNAGC	1 cut(s)	343
Bpu14I	TTCGAA	1 cut(s)	1700
BpuEI	CTTGAG	5 cut(s)	36, 286, 694, 905, 1149
Bsa29I	ATCGAT	1 cut(s)	1140
BsaAI	YACGTR	1 cut(s)	1780
BsaBI	GATNNNNATC	2 cut(s)	23, 1593
BsaJI	CCNNGG	6 cut(s)	103, 262, 833, 1193, 1301, 1684
BsaWI	WCCGGW	1 cut(s)	1367
BsaXI	ACNNNNNCTCC	2 cut(s)	1664, 1694
Bsc4I	CCNNNNNNNGG	8 cut(s)	56, 90, 108, 109, 505, 1302, 1330, 1384
Bse118I	RCCGGY	2 cut(s)	598, 1066
Bse1I	ACTGG	1 cut(s)	1378
Bse3DI	GCAATG	2 cut(s)	634, 1835
Bse8I	GATNNNNATC	2 cut(s)	23, 1593
BseAI	TCCGGA	1 cut(s)	1367
BseBI	CCWGG	3 cut(s)	1059, 1104, 1152
BseCI	ATCGAT	1 cut(s)	1140
BseDI	CCNNGG	6 cut(s)	103, 262, 833, 1193, 1301, 1684
BseGI	GGATG	5 cut(s)	154, 337, 1234, 1555, 1711
BseJI	GATNNNNATC	2 cut(s)	23, 1593
BseLI	CCNNNNNNNGG	8 cut(s)	56, 90, 108, 109, 505, 1302, 1330, 1384
BseMI	GCAATG	2 cut(s)	634, 1835
BseMII	CTCAG	6 cut(s)	215, 285, 327, 477, 992, 1763
BseNI	ACTGG	1 cut(s)	1378
BseRI	GAGGAG	2 cut(s)	114, 149
BseSI	GKGCMC	2 cut(s)	705, 1204
BseXI	GCAGC	6 cut(s)	25, 550, 556, 578, 1324, 1493
BseYI	CCCAGC	1 cut(s)	723
BsgI	GTGCAG	2 cut(s)	555, 1143
Bsh1236I	CGCG	1 cut(s)	387
BshFI	GGCC	8 cut(s)	5, 443, 598, 639, 693, 838, 1006, 1754
BshNI	GGYRCC	3 cut(s)	550, 731, 1290
BshVI	ATCGAT	1 cut(s)	1140
BsiHKAI	GWGCWC	2 cut(s)	423, 1204
BsiHKCI	CYCGRG	2 cut(s)	102, 648
BsiSI	CCGG	4 cut(s)	382, 599, 1067, 1368
BslFI	GGGAC	3 cut(s)	280, 378, 769
BslI	CCNNNNNNNGG	8 cut(s)	56, 90, 108, 109, 505, 1302, 1330, 1384
BsmFI	GGGAC	3 cut(s)	280, 378, 769
BsmI	GAATGC	1 cut(s)	376
BsnI	GGCC	8 cut(s)	5, 443, 598, 639, 693, 838, 1006, 1754
BsoBI	CYCGRG	2 cut(s)	102, 648
Bsp119I	TTCGAA	1 cut(s)	1700
Bsp1286I	GDGCHC	4 cut(s)	103, 423, 705, 1204
Bsp13I	TCCGGA	1 cut(s)	1367
Bsp143I	GATC	5 cut(s)	18, 214, 964, 1364, 1720
Bsp1720I	GCTNAGC	1 cut(s)	343
BspACI	CCGC	4 cut(s)	16, 521, 942, 1600
BspANI	GGCC	8 cut(s)	5, 443, 598, 639, 693, 838, 1006, 1754
BspCNI	CTCAG	6 cut(s)	216, 286, 328, 478, 993, 1764
BspDI	ATCGAT	1 cut(s)	1140
BspEI	TCCGGA	1 cut(s)	1367
BspFNI	CGCG	1 cut(s)	387
BspLI	GGNNCC	6 cut(s)	20, 48, 552, 733, 1292, 1722
BspMAI	CTGCAG	3 cut(s)	15, 568, 1483
BspMI	ACCTGC	3 cut(s)	583, 1486, 1789
BspPI	GGATC	6 cut(s)	13, 26, 209, 1359, 1715, 1728
BspQI	GCTCTTC	1 cut(s)	40
BspT104I	TTCGAA	1 cut(s)	1700
BspT107I	GGYRCC	3 cut(s)	550, 731, 1290
BspTI	CTTAAG	1 cut(s)	1328
BsrDI	GCAATG	2 cut(s)	634, 1835
BsrFI	RCCGGY	2 cut(s)	598, 1066
BsrI	ACTGG	1 cut(s)	1378
BssAI	RCCGGY	2 cut(s)	598, 1066
BssECI	CCNNGG	6 cut(s)	103, 262, 833, 1193, 1301, 1684
BssMI	GATC	5 cut(s)	18, 214, 964, 1364, 1720
BssT1I	CCWWGG	5 cut(s)	262, 833, 1193, 1301, 1684
Bst2UI	CCWGG	3 cut(s)	1059, 1104, 1152
Bst4CI	ACNGT	3 cut(s)	415, 1505, 1767
Bst6I	CTCTTC	2 cut(s)	40, 1217
BstAFI	CTTAAG	1 cut(s)	1328
BstBAI	YACGTR	1 cut(s)	1780
BstBI	TTCGAA	1 cut(s)	1700
BstC8I	GCNNGC	2 cut(s)	549, 679
BstENI	CCTNNNNNAGG	2 cut(s)	54, 1300
BstF5I	GGATG	5 cut(s)	154, 337, 1234, 1555, 1711
BstFNI	CGCG	1 cut(s)	387
BstHHI	GCGC	1 cut(s)	387
BstKTI	GATC	5 cut(s)	21, 217, 967, 1367, 1723
BstMBI	GATC	5 cut(s)	18, 214, 964, 1364, 1720
BstMWI	GCNNNNNNNGC	5 cut(s)	563, 569, 575, 584, 1481
BstNI	CCWGG	3 cut(s)	1059, 1104, 1152
BstNSI	RCATGY	2 cut(s)	681, 690
BstSCI	CCNGG	3 cut(s)	1057, 1102, 1150
BstSFI	CTRYAG	4 cut(s)	11, 564, 1479, 1763
BstSLI	GKGCMC	2 cut(s)	705, 1204
BstSNI	TACGTA	1 cut(s)	1780
BstUI	CGCG	1 cut(s)	387
BstV1I	GCAGC	6 cut(s)	25, 550, 556, 578, 1324, 1493
BstV2I	GAAGAC	1 cut(s)	416
BstX2I	RGATCY	4 cut(s)	18, 214, 1364, 1720
BstXI	CCANNNNNNTGG	1 cut(s)	1576
BstYI	RGATCY	4 cut(s)	18, 214, 1364, 1720
Bsu15I	ATCGAT	1 cut(s)	1140
BsuRI	GGCC	8 cut(s)	5, 443, 598, 639, 693, 838, 1006, 1754
BsuTUI	ATCGAT	1 cut(s)	1140
BtgZI	GCGATG	1 cut(s)	402
BtsCI	GGATG	5 cut(s)	154, 337, 1234, 1555, 1711
BtsI	GCAGTG	1 cut(s)	1746
BtsIMutI	CAGTG	2 cut(s)	365, 1746
BveI	ACCTGC	3 cut(s)	583, 1486, 1789
Cac8I	GCNNGC	2 cut(s)	549, 679
CaiI	CAGNNNCTG	2 cut(s)	1157, 1771
CfoI	GCGC	1 cut(s)	387
Cfr10I	RCCGGY	2 cut(s)	598, 1066
Cfr13I	GGNCC	5 cut(s)	4, 46, 637, 1582, 1752
ClaI	ATCGAT	1 cut(s)	1140
CseI	GACGC	1 cut(s)	771
CsiI	ACCWGGT	1 cut(s)	1150
Csp6I	GTAC	7 cut(s)	720, 732, 1155, 1185, 1291, 1519, 1777
CviAII	CATG	7 cut(s)	641, 678, 687, 1013, 1453, 1586, 1691
CviQI	GTAC	7 cut(s)	720, 732, 1155, 1185, 1291, 1519, 1777
DpnI	GATC	5 cut(s)	20, 216, 966, 1366, 1722
DpnII	GATC	5 cut(s)	18, 214, 964, 1364, 1720
DraIII	CACNNNGTG	1 cut(s)	875
EaeI	YGGCCR	2 cut(s)	441, 691
Eam1104I	CTCTTC	2 cut(s)	40, 1217
EarI	CTCTTC	2 cut(s)	40, 1217
Ecl136II	GAGCTC	1 cut(s)	421
Eco105I	TACGTA	1 cut(s)	1780
Eco130I	CCWWGG	5 cut(s)	262, 833, 1193, 1301, 1684
Eco147I	AGGCCT	1 cut(s)	838
Eco24I	GRGCYC	2 cut(s)	103, 423
Eco32I	GATATC	1 cut(s)	1373
Eco47I	GGWCC	2 cut(s)	46, 1582
Eco53kI	GAGCTC	1 cut(s)	421
Eco57I	CTGAAG	1 cut(s)	428
Eco88I	CYCGRG	2 cut(s)	102, 648
EcoICRI	GAGCTC	1 cut(s)	421
EcoNI	CCTNNNNNAGG	2 cut(s)	54, 1300
EcoRI	GAATTC	1 cut(s)	245
EcoRII	CCWGG	3 cut(s)	1057, 1102, 1150
EcoRV	GATATC	1 cut(s)	1373
EcoT14I	CCWWGG	5 cut(s)	262, 833, 1193, 1301, 1684
EcoT38I	GRGCYC	2 cut(s)	103, 423
ErhI	CCWWGG	5 cut(s)	262, 833, 1193, 1301, 1684
FaeI	CATG	7 cut(s)	644, 681, 690, 1016, 1456, 1589, 1694
FaqI	GGGAC	3 cut(s)	280, 378, 769
FatI	CATG	7 cut(s)	640, 677, 686, 1012, 1452, 1585, 1690
FauI	CCCGC	1 cut(s)	514
FauNDI	CATATG	1 cut(s)	29
FblI	GTMKAC	1 cut(s)	79
Fnu4HI	GCNGC	6 cut(s)	14, 564, 567, 570, 1338, 1482
FokI	GGATG	5 cut(s)	161, 344, 1241, 1562, 1718
FriOI	GRGCYC	2 cut(s)	103, 423
Fsp4HI	GCNGC	6 cut(s)	14, 564, 567, 570, 1338, 1482
GlaI	GCGC	1 cut(s)	386
GluI	GCNGC	6 cut(s)	14, 564, 567, 570, 1338, 1482
GsaI	CCCAGC	1 cut(s)	727
HaeIII	GGCC	8 cut(s)	5, 443, 598, 639, 693, 838, 1006, 1754
HapII	CCGG	4 cut(s)	382, 599, 1067, 1368
HgaI	GACGC	1 cut(s)	771
HhaI	GCGC	1 cut(s)	387
Hin1II	CATG	7 cut(s)	644, 681, 690, 1016, 1456, 1589, 1694
Hin6I	GCGC	1 cut(s)	385
HinP1I	GCGC	1 cut(s)	385
HinfI	GANTC	6 cut(s)	118, 463, 479, 829, 1710, 1814
HpaII	CCGG	4 cut(s)	382, 599, 1067, 1368
HphI	GGTGA	4 cut(s)	52, 1285, 1367, 1399
Hpy166II	GTNNAC	5 cut(s)	80, 939, 1129, 1202, 1235
Hpy188I	TCNGA	3 cut(s)	225, 404, 1811
Hpy188III	TCNNGA	6 cut(s)	650, 1082, 1094, 1348, 1368, 1648
Hpy8I	GTNNAC	5 cut(s)	80, 939, 1129, 1202, 1235
HpyAV	CCTTC	3 cut(s)	1113, 1696, 1734
HpyCH4III	ACNGT	3 cut(s)	415, 1505, 1767
HpyCH4IV	ACGT	2 cut(s)	1574, 1779
HpyF10VI	GCNNNNNNNGC	5 cut(s)	563, 569, 575, 584, 1481
HpySE526I	ACGT	2 cut(s)	1574, 1779
Hsp92II	CATG	7 cut(s)	644, 681, 690, 1016, 1456, 1589, 1694
HspAI	GCGC	1 cut(s)	385
Kpn2I	TCCGGA	1 cut(s)	1367
KpnI	GGTACC	2 cut(s)	735, 1294
Kzo9I	GATC	5 cut(s)	18, 214, 964, 1364, 1720
LguI	GCTCTTC	1 cut(s)	40
LmnI	GCTCC	4 cut(s)	229, 418, 525, 779
Lsp1109I	GCAGC	6 cut(s)	25, 550, 556, 578, 1324, 1493
LweI	GCATC	4 cut(s)	170, 322, 895, 1603
MabI	ACCWGGT	1 cut(s)	1150
MaeII	ACGT	2 cut(s)	1574, 1779
MaeIII	GTNAC	2 cut(s)	1273, 1767
MalI	GATC	5 cut(s)	20, 216, 966, 1366, 1722
MboI	GATC	5 cut(s)	18, 214, 964, 1364, 1720
MboII	GAAGA	5 cut(s)	27, 421, 1231, 1234, 1363
MfeI	CAATTG	2 cut(s)	622, 915
MflI	RGATCY	4 cut(s)	18, 214, 1364, 1720
MhlI	GDGCHC	4 cut(s)	103, 423, 705, 1204
MlsI	TGGCCA	2 cut(s)	443, 693
MluCI	AATT	7 cut(s)	245, 622, 915, 1631, 1643, 1658, 1747
MluNI	TGGCCA	2 cut(s)	443, 693
MlyI	GAGTC	2 cut(s)	457, 823
MmeI	TCCRAC	1 cut(s)	1557
Mox20I	TGGCCA	2 cut(s)	443, 693
MroI	TCCGGA	1 cut(s)	1367
MroXI	GAANNNNTTC	1 cut(s)	617
MscI	TGGCCA	2 cut(s)	443, 693
MseI	TTAA	4 cut(s)	432, 1281, 1329, 1466
MslI	CAYNNNNRTG	1 cut(s)	972
Msp20I	TGGCCA	2 cut(s)	443, 693
MspA1I	CMGCKG	2 cut(s)	16, 569
MspCI	CTTAAG	1 cut(s)	1328
MspI	CCGG	4 cut(s)	382, 599, 1067, 1368
MspR9I	CCNGG	3 cut(s)	1059, 1104, 1152
MunI	CAATTG	2 cut(s)	622, 915
Mva1269I	GAATGC	1 cut(s)	376
MvaI	CCWGG	3 cut(s)	1059, 1104, 1152
MvnI	CGCG	1 cut(s)	387
MwoI	GCNNNNNNNGC	5 cut(s)	563, 569, 575, 584, 1481
NdeI	CATATG	1 cut(s)	29
NdeII	GATC	5 cut(s)	18, 214, 964, 1364, 1720
NlaIII	CATG	7 cut(s)	644, 681, 690, 1016, 1456, 1589, 1694
NlaIV	GGNNCC	6 cut(s)	20, 48, 552, 733, 1292, 1722
NmuCI	GTSAC	1 cut(s)	1273
NspI	RCATGY	2 cut(s)	681, 690
NspV	TTCGAA	1 cut(s)	1700
PaeI	GCATGC	1 cut(s)	681
PaeR7I	CTCGAG	1 cut(s)	648
PaqCI	CACCTGC	2 cut(s)	583, 1486
PceI	AGGCCT	1 cut(s)	838
PciI	ACATGT	1 cut(s)	686
PciSI	GCTCTTC	1 cut(s)	40
PcsI	WCGNNNNNNNCGW	1 cut(s)	1653
PctI	GAATGC	1 cut(s)	376
PdmI	GAANNNNTTC	1 cut(s)	617
PfeI	GAWTC	4 cut(s)	118, 479, 1710, 1814
PkrI	GCNGC	6 cut(s)	15, 565, 568, 571, 1339, 1483
PleI	GAGTC	2 cut(s)	457, 823
PpsI	GAGTC	2 cut(s)	457, 823
Ppu21I	YACGTR	1 cut(s)	1780
PscI	ACATGT	1 cut(s)	686
Psp124BI	GAGCTC	1 cut(s)	423
Psp6I	CCWGG	3 cut(s)	1057, 1102, 1150
PspFI	CCCAGC	1 cut(s)	723
PspGI	CCWGG	3 cut(s)	1057, 1102, 1150
PspN4I	GGNNCC	6 cut(s)	20, 48, 552, 733, 1292, 1722
PspPI	GGNCC	5 cut(s)	4, 46, 637, 1582, 1752
PstI	CTGCAG	3 cut(s)	15, 568, 1483
PstNI	CAGNNNCTG	2 cut(s)	1157, 1771
PsuI	RGATCY	4 cut(s)	18, 214, 1364, 1720
PvuII	CAGCTG	1 cut(s)	569
RsaI	GTAC	7 cut(s)	721, 733, 1156, 1186, 1292, 1520, 1778
RsaNI	GTAC	7 cut(s)	720, 732, 1155, 1185, 1291, 1519, 1777
RseI	CAYNNNNRTG	1 cut(s)	972
SacI	GAGCTC	1 cut(s)	423
SapI	GCTCTTC	1 cut(s)	40
SaqAI	TTAA	4 cut(s)	432, 1281, 1329, 1466
SatI	GCNGC	6 cut(s)	14, 564, 567, 570, 1338, 1482
Sau3AI	GATC	5 cut(s)	18, 214, 964, 1364, 1720
Sau96I	GGNCC	5 cut(s)	4, 46, 637, 1582, 1752
ScaI	AGTACT	1 cut(s)	1186
SchI	GAGTC	2 cut(s)	457, 823
ScrFI	CCNGG	3 cut(s)	1059, 1104, 1152
SduI	GDGCHC	4 cut(s)	103, 423, 705, 1204
SexAI	ACCWGGT	1 cut(s)	1150
SfaNI	GCATC	4 cut(s)	170, 322, 895, 1603
SfcI	CTRYAG	4 cut(s)	11, 564, 1479, 1763
Sfr274I	CTCGAG	1 cut(s)	648
SfuI	TTCGAA	1 cut(s)	1700
SinI	GGWCC	2 cut(s)	46, 1582
SlaI	CTCGAG	1 cut(s)	648
SmiMI	CAYNNNNRTG	1 cut(s)	972
SmlI	CTYRAG	7 cut(s)	51, 301, 648, 709, 884, 1164, 1328
SmoI	CTYRAG	7 cut(s)	51, 301, 648, 709, 884, 1164, 1328
SnaBI	TACGTA	1 cut(s)	1780
SphI	GCATGC	1 cut(s)	681
Sse9I	AATT	7 cut(s)	245, 622, 915, 1631, 1643, 1658, 1747
SseBI	AGGCCT	1 cut(s)	838
SsiI	CCGC	4 cut(s)	16, 521, 942, 1600
SstI	GAGCTC	1 cut(s)	423
StuI	AGGCCT	1 cut(s)	838
StyD4I	CCNGG	3 cut(s)	1057, 1102, 1150
StyI	CCWWGG	5 cut(s)	262, 833, 1193, 1301, 1684
TaaI	ACNGT	3 cut(s)	415, 1505, 1767
TaiI	ACGT	2 cut(s)	1577, 1782
TaqI	TCGA	8 cut(s)	73, 144, 649, 1140, 1320, 1349, 1647, 1700
TasI	AATT	7 cut(s)	245, 622, 915, 1631, 1643, 1658, 1747
TatI	WGTACW	1 cut(s)	1184
TfiI	GAWTC	4 cut(s)	118, 479, 1710, 1814
Tru1I	TTAA	4 cut(s)	432, 1281, 1329, 1466
Tru9I	TTAA	4 cut(s)	432, 1281, 1329, 1466
TscAI	CASTG	2 cut(s)	365, 1746
TseFI	GTSAC	1 cut(s)	1273
TseI	GCWGC	6 cut(s)	13, 563, 566, 569, 1337, 1481
Tsp45I	GTSAC	1 cut(s)	1273
TspGWI	ACGGA	1 cut(s)	1506
TspRI	CASTG	2 cut(s)	365, 1746
Vha464I	CTTAAG	1 cut(s)	1328
VneI	GTGCAC	1 cut(s)	1200
VpaK11BI	GGWCC	2 cut(s)	46, 1582
XagI	CCTNNNNNAGG	2 cut(s)	54, 1300
XapI	RAATTY	3 cut(s)	245, 1643, 1658
XceI	RCATGY	2 cut(s)	681, 690
XhoI	CTCGAG	1 cut(s)	648
XmaJI	CCTAGG	2 cut(s)	833, 1301
XmiI	GTMKAC	1 cut(s)	79
XmnI	GAANNNNTTC	1 cut(s)	617
ZrmI	AGTACT	1 cut(s)	1186

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.