Rw4G005960

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000094
Physical Location & Seq
Reverse (-)
12046458 .. 12049895
3438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G005960.1

Sequence Viewer

Length: 1929 bp
ATGGACATTGAGTCAGACTCACCTGCTGGATTTCACCCTAGGGATCCTGGTGGCCCTAGCCAGGGTCCATCTAAGAGGGCTACTTCCACATCTGGTTCGGGTTCTTCAGTTGGTAGTGGGAAGAGCAGTGGTTCCAGTTCGAGGACCCGTACTAGGTTCAGGGTACGTGACAGGAGATTCTCTCGGGGCCAGTCTAGTGGACGACAGTTTGGACAGTTTGAGAGGTCCAGGAGTTATCATGGTGGTTCGAGTGGGGCTTCTGCTAGCCAGCCAGCCCAGTTTGGACAGTATCAGACAGCAGGATGTTTTATTTGTGGTCAGCAGGATCACTTCAGGAGGGACTGTCCCCTTGCGACTCAGGGAGCTAGATCTACCCCTACTCAGACTGTTGGTCAGTCCTCTGCTGACCTTGTGGAGTTTGATGTAATTTTGGGGATGGACTTTTTGGAGACACATGGTGCATTGGTTGATTGTTTCCGCAAAGAAGTAGTGTTTCGAAGTCCAGGAAAACCGGAAATCACCTTCCGTGGTGAGAGGAACATTCTCTCCTCTTGCCTGATTTCGGCCATTACGGCTGAAAAGCTTATGAATAAAGGTTGTCAGGCTTATTTAGCACACATTTTTCCGGATGTGTTTCCCGATGAACTACCTGGTTTACCTCCAGAAAGGGAAATAGATTTTACCATTGAGTTGCTCCCTGCCCCCCTCACTAAATTGACCAGGAAAGGTGCTAAATTTGTTTGGTCGGATGAGTGTGAGCAAAGTTTCCAAGAACTCAAAAGACGTTTAACTTGTGCCCCAGTTTTAGTATTGCCTGATGATAGCGGGGAGTATGTGATTTACAGTGATGCCTCAAGACAAGGTTTAGGTTGTGTGTTGATGCAACATGGGAATTATCTACTTAATCAAAGGGACTTAAATTTGAGGCAAAGGAGGTGGTTAGAGTTGATTAAGGACTATGATTGCACAATTGAGTACCATCCAGGAAGGGCTAATGTGGTAGCTGATGCACTTAGCCGAAAGCCCTCTAGCTCTTTAGCCCATTTGAAGACAGTTCGAGTCCCTTTACTTTATGCATTGCGATCTACAGGAGTTAATTTGGCAATTGAAGAAGGGTCTGGAGCATTGTTAGCTAGCTTCCATGTGAGGCCGAATCTCATTGATAAGGTGCGGGAGGCACAAGATGAAGACGAATATTTAAGACAAGTAAGAGAAGCAGTGAGTGATGGCATTAGAACAGACTTTATTCTTAGAGGAGATGGAGCCTTAATGTTTGAGAATAGAATATGTGTACCTAATCTTCCTGATATTAAACGAGAAATTTTGGATGAGGCTCATAGTTCTGCTTACGCTATGCATCCTGGCGGGACAAAAATGTATCGAACTTTAAAACCATATTATTGGTGGCGGAATATGAAAAGGGAGATTGCAAATATTTGGGTGATTGTGGATCGACTCACCAAGTCTGCTCATTTCTTACCGGTTAACAAGACTTATGGGATGAACAAGCTGGCGGAACTTTATGTAAATGAAATCGTGAAACTTCATGGACCACCCGTTTCCATTGTCTCTGATCGAGATCCTCGTTTCACCTCAAAATTTTGGAAGAGTCTACACGAGGCTTTAGGTACTGGATTAAGTTTTAGCACGGCATTTCATCCACAGACTGATGGGCAATCTGAGAGGACTATTCAAACTTTGGAAGACATGTTGAGATCCTGTGTTATGCAATTCAAAGGAAGTTGGGATAGCCATCTGGCATTGATTGAGTTCGCTTACAATAATAGTTATCATTCCAGTATTGACATGGCTCCTTACGAAGCTCTCTATGGAAGACAATGTCGTACACCCTTATGTTGGAATGAAGTGGGAGAAAGGAAGCTCATAGGTCCAGAGATTGTACGGATTACCACTGAGAAGTTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve zf-CCHC

Protein Analysis

642

Amino Acids

71.98

Weight (kDa)

8.42

Isoelectric Point (pI)

52.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH_2 PF17919 248 - 298 1.7e-13 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 437 - 478 5.4e-10 Integrase zinc binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000094)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42190
fragaria_vesca FvH4_1g22371 FvH4_1g23261 FvH4_1g27933 FvH4_1g27934 FvH4_1g29231 FvH4_2g02781 FvH4_2g08031 FvH4_2g08581 FvH4_3g06961 FvH4_3g06962 FvH4_3g13531 FvH4_3g14391 FvH4_3g14392 FvH4_3g18622 FvH4_3g18964 FvH4_3g26314 FvH4_3g28781 FvH4_3g30771 FvH4_3g30772 FvH4_3g31752 FvH4_3g32803 FvH4_3g35751 FvH4_3g35752 FvH4_4g02151 FvH4_4g02642 FvH4_4g05523 FvH4_4g05524 FvH4_4g06574 FvH4_4g06722 FvH4_4g08644 FvH4_4g08645 FvH4_4g08646 FvH4_4g08851 FvH4_4g08971 FvH4_4g09081 FvH4_4g09243 FvH4_4g09244 FvH4_4g09283 FvH4_4g10021 FvH4_4g10032 FvH4_4g10033 FvH4_4g10182 FvH4_4g10361 FvH4_4g12502 FvH4_4g12503 FvH4_4g13341 FvH4_4g13942 FvH4_4g13943 FvH4_4g13944 FvH4_4g14432 FvH4_4g14822 FvH4_4g14823 FvH4_4g15291 FvH4_4g15292 FvH4_4g15293 FvH4_4g15471 FvH4_4g15583 FvH4_4g15584 FvH4_4g15585 FvH4_4g15586 FvH4_4g15587 FvH4_4g15591 FvH4_4g15592 FvH4_4g15593 FvH4_4g16179 FvH4_4g18131 FvH4_4g21031 FvH4_4g22021 FvH4_4g22021 FvH4_4g22042 FvH4_5g03402 FvH4_5g07411 FvH4_5g13881 FvH4_5g15792 FvH4_5g24682 FvH4_5g24911 FvH4_5g25322 FvH4_5g25641 FvH4_5g36692 FvH4_5g38072 FvH4_5g38171 FvH4_6g05202 FvH4_6g08531 FvH4_6g16342 FvH4_6g21771 FvH4_6g23303 FvH4_6g23961 FvH4_6g25381 FvH4_6g27021 FvH4_6g27022 FvH4_6g30091 FvH4_6g30092 FvH4_7g02051 FvH4_7g06031 FvH4_7g07031 FvH4_7g07581 FvH4_7g08381 FvH4_7g11551 FvH4_7g19711 FvH4_7g25621 FvH4_7g29722
malus_domestica MD06G1134500.v1.1 MD09G1067600.v1.1 MD13G1145500.v1.1 MD15G1166500.v1.1 MD16G1270000.v1.1
prunus_persica Prupe.7G030900_v2.0.a1
pyrus_communis pycom01g00710 pycom01g03760 pycom03g10770 pycom04g02180 pycom05g08050 pycom06g04140 pycom06g11110 pycom07g07550 pycom07g21570 pycom08g17240 pycom09g13900 pycom09g19570 pycom10g22300 pycom11g03210 pycom11g13750 pycom11g24890 pycom12561g00270 pycom12668g00020 pycom12g11280 pycom12g20720 pycom12g20730 pycom13g21410 pycom13g21660 pycom14g06010 pycom14g06370 pycom14g12800 pycom16g21440 pycom16g22530 pycom17g02030 pycom420g00170 pycom50g00020 pycom5153684g00110 pycom520g00860 pycom581g00060
rosa_chinensis RchiOBHm_Chr0c08g0498091 RchiOBHm_Chr0c08g0498101 RchiOBHm_Chr1g0326991 RchiOBHm_Chr2g0084311 RchiOBHm_Chr2g0090701 RchiOBHm_Chr2g0098591 RchiOBHm_Chr2g0109491 RchiOBHm_Chr2g0109501 RchiOBHm_Chr2g0130801 RchiOBHm_Chr2g0145931 RchiOBHm_Chr2g0168091 RchiOBHm_Chr3g0457081 RchiOBHm_Chr3g0457091 RchiOBHm_Chr3g0467781 RchiOBHm_Chr3g0467791 RchiOBHm_Chr3g0493771 RchiOBHm_Chr3g0494051 RchiOBHm_Chr3g0496881 RchiOBHm_Chr4g0401391 RchiOBHm_Chr4g0414861 RchiOBHm_Chr4g0424081 RchiOBHm_Chr5g0009611 RchiOBHm_Chr5g0049881 RchiOBHm_Chr5g0050351 RchiOBHm_Chr5g0053791 RchiOBHm_Chr5g0078031 RchiOBHm_Chr6g0256481 RchiOBHm_Chr6g0271231 RchiOBHm_Chr6g0286381 RchiOBHm_Chr7g0197101 RchiOBHm_Chr7g0221891 RchiOBHm_Chr7g0237801 RchiOBHm_Chr7g0237821
rosa_multiflora Rmu_sc0000509.1_g000034 Rmu_sc0004751.1_g000014 Rmu_sc0008546.1_g000002 Rmu_sc0009320.1_g000003 Rmu_sc0010661.1_g000006 Rmu_sc0029652.1_g000002
rosa_roxburghii Rroxscaffold_10G00433150 Rroxscaffold_15G00447630 Rroxscaffold_26G00447790 Rroxscaffold_29G00441810 Rroxscaffold_32G00442860 Rroxscaffold_33G00439800 Rroxscaffold_34G00443230 Rroxscaffold_3G00220560 Rroxscaffold_3G00227370 Rroxscaffold_3G00239080 Rroxscaffold_3G00239090 Rroxscaffold_3G00273190 Rroxscaffold_4G00323530 Rroxscaffold_4G00326840 Rroxscaffold_57G00443110 Rroxscaffold_5G00338590 Rroxscaffold_5G00347530 Rroxscaffold_68G00446990 Rroxscaffold_76G00448570 Rroxscaffold_90G00448860
rosa_rugosa Rorug06G0233600
rosa_samantha Rh3CG302700 Rh6DG286700
rosa_wichuraiana Rw0G002280 Rw0G004080 Rw0G011830 Rw1G003690 Rw1G008800 Rw1G009130 Rw1G009140 Rw1G009150 Rw1G009160 Rw1G009410 Rw1G009580 Rw1G012770 Rw1G015850 Rw1G026860 Rw2G003780 Rw2G006480 Rw2G016520 Rw2G016960 Rw2G018170 Rw2G025180 Rw2G025510 Rw2G025610 Rw2G025750 Rw2G025920 Rw2G029950 Rw2G046360 Rw2G046370 Rw2G046380 Rw3G009070 Rw3G025820 Rw3G027340 Rw3G027900 Rw3G028150 Rw3G029060 Rw4G002440 Rw4G005960 Rw4G007520 Rw4G011970 Rw4G011980 Rw4G012190 Rw4G012530 Rw4G018490 Rw4G026380 Rw5G003970 Rw5G030920 Rw5G031780 Rw5G031900 Rw5G033030 Rw5G033040 Rw5G036330 Rw5G039730 Rw5G048110 Rw6G006670 Rw6G007910 Rw6G009720 Rw6G010990 Rw6G012930 Rw6G012940 Rw6G013420 Rw6G014690 Rw6G015290 Rw6G015790 Rw6G015920 Rw6G036510 Rw7G016650 Rw7G023140 Rw7G028650 Rw7G036450 Rw7G036460 Rw7G036910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 31
Acc36I ACCTGC 1 cut(s) 31
AccI GTMKAC 1 cut(s) 1612
AccIII TCCGGA 1 cut(s) 625
AciI CCGC 6 cut(s) 478, 825, 1171, 1365, 1408, 1514
AclWI GGATC 6 cut(s) 38, 51, 333, 1458, 1574, 1710
AcoI YGGCCR 1 cut(s) 564
AcsI RAATTY 4 cut(s) 734, 919, 1320, 1598
AcuI CTGAAG 2 cut(s) 90, 316
AdeI CACNNNGTG 1 cut(s) 458
AfaI GTAC 7 cut(s) 151, 165, 977, 1293, 1630, 1846, 1902
AfiI CCNNNNNNNGG 6 cut(s) 61, 62, 141, 153, 562, 1857
AflIII ACRYGT 1 cut(s) 1707
AgeI ACCGGT 1 cut(s) 1480
AgsI TTSAA 4 cut(s) 1048, 1109, 1694, 1735
AhdI GACNNNNNGTC 2 cut(s) 10, 390
AjnI CCWGG 8 cut(s) 46, 60, 227, 502, 649, 719, 982, 1360
AloI GAACNNNNNNTCC 2 cut(s) 530, 562
AluBI AGCT 9 cut(s) 365, 583, 1004, 1032, 1133, 1137, 1510, 1823, 1882
AluI AGCT 9 cut(s) 365, 583, 1004, 1032, 1133, 1137, 1510, 1823, 1882
Alw26I GTCTC 2 cut(s) 443, 1573
AlwI GGATC 6 cut(s) 38, 51, 333, 1458, 1574, 1710
Ama87I CYCGRG 1 cut(s) 183
Aor13HI TCCGGA 1 cut(s) 625
AoxI GGCC 4 cut(s) 52, 187, 564, 1148
ApoI RAATTY 4 cut(s) 734, 919, 1320, 1598
AsiGI ACCGGT 1 cut(s) 1480
AspA2I CCTAGG 1 cut(s) 38
AspS9I GGNCC 7 cut(s) 53, 65, 144, 187, 225, 1550, 1889
AsuHPI GGTGA 7 cut(s) 12, 26, 511, 542, 1450, 1453, 1582
AsuII TTCGAA 1 cut(s) 496
AsuNHI GCTAGC 2 cut(s) 263, 1133
AvaI CYCGRG 1 cut(s) 183
AvaII GGWCC 5 cut(s) 65, 144, 225, 1550, 1889
AvrII CCTAGG 1 cut(s) 38
BaeGI GKGCMC 1 cut(s) 799
BamHI GGATCC 1 cut(s) 43
BarI GAAGNNNNNNTAC 2 cut(s) 1284, 1316
BauI CACGAG 1 cut(s) 1616
BbsI GAAGAC 4 cut(s) 1055, 1194, 1710, 1840
BccI CCATC 7 cut(s) 76, 430, 987, 1220, 1253, 1664, 1761
BceAI ACGGC 2 cut(s) 588, 1665
BciT130I CCWGG 8 cut(s) 48, 62, 229, 504, 651, 721, 984, 1362
BcoDI GTCTC 2 cut(s) 443, 1573
BfaI CTAG 8 cut(s) 39, 57, 153, 195, 264, 366, 1029, 1134
BfmI CTRYAG 1 cut(s) 1086
BfuAI ACCTGC 1 cut(s) 31
BglI GCCNNNNNGGC 1 cut(s) 572
BglII AGATCT 1 cut(s) 368
BlnI CCTAGG 1 cut(s) 38
Bme1390I CCNGG 8 cut(s) 48, 62, 229, 504, 651, 721, 984, 1362
Bme18I GGWCC 5 cut(s) 65, 144, 225, 1550, 1889
BmeRI GACNNNNNGTC 2 cut(s) 10, 390
BmeT110I CYCGRG 1 cut(s) 183
BmgT120I GGNCC 7 cut(s) 53, 65, 144, 187, 225, 1550, 1889
BmiI GGNNCC 7 cut(s) 45, 66, 133, 146, 188, 1264, 1812
BmrFI CCNGG 8 cut(s) 48, 62, 229, 504, 651, 721, 984, 1362
BmrI ACTGGG 2 cut(s) 271, 794
BmsI GCATC 4 cut(s) 838, 870, 997, 1366
BmtI GCTAGC 2 cut(s) 267, 1137
BmuI ACTGGG 2 cut(s) 271, 794
BpiI GAAGAC 4 cut(s) 1055, 1194, 1710, 1840
BplI GAGNNNNNCTC 3 cut(s) 34, 166, 198
BpmI CTGGAG 2 cut(s) 645, 1140
Bpu14I TTCGAA 1 cut(s) 496
BpuEI CTTGAG 1 cut(s) 838
BsaAI YACGTR 1 cut(s) 167
BsaBI GATNNNNATC 2 cut(s) 1578, 1752
BsaJI CCNNGG 3 cut(s) 38, 61, 526
BsaWI WCCGGW 3 cut(s) 511, 625, 1480
BsaXI ACNNNNNCTCC 4 cut(s) 328, 358, 530, 560
Bsc4I CCNNNNNNNGG 6 cut(s) 61, 62, 141, 153, 562, 1857
Bse118I RCCGGY 1 cut(s) 1480
Bse1I ACTGG 6 cut(s) 135, 190, 277, 800, 1636, 1797
Bse3DI GCAATG 1 cut(s) 1076
Bse8I GATNNNNATC 2 cut(s) 1578, 1752
BseAI TCCGGA 1 cut(s) 625
BseBI CCWGG 8 cut(s) 48, 62, 229, 504, 651, 721, 984, 1362
BseDI CCNNGG 3 cut(s) 38, 61, 526
BseGI GGATG 9 cut(s) 308, 441, 634, 754, 979, 1333, 1357, 1506, 1657
BseJI GATNNNNATC 2 cut(s) 1578, 1752
BseLI CCNNNNNNNGG 6 cut(s) 61, 62, 141, 153, 562, 1857
BseMI GCAATG 1 cut(s) 1076
BseMII CTCAG 4 cut(s) 371, 395, 1671, 1905
BseNI ACTGG 6 cut(s) 135, 190, 277, 800, 1636, 1797
BseRI GAGGAG 2 cut(s) 538, 1269
BseSI GKGCMC 1 cut(s) 799
BshFI GGCC 4 cut(s) 54, 189, 566, 1150
BshTI ACCGGT 1 cut(s) 1480
BsiHKCI CYCGRG 1 cut(s) 183
BsiSI CCGG 3 cut(s) 512, 626, 1481
BslFI GGGAC 5 cut(s) 330, 353, 926, 1046, 1381
BslI CCNNNNNNNGG 6 cut(s) 61, 62, 141, 153, 562, 1857
BsmAI GTCTC 2 cut(s) 443, 1573
BsmFI GGGAC 5 cut(s) 330, 353, 926, 1046, 1381
BsnI GGCC 4 cut(s) 54, 189, 566, 1150
BsoBI CYCGRG 1 cut(s) 183
Bsp119I TTCGAA 1 cut(s) 496
Bsp1286I GDGCHC 1 cut(s) 799
Bsp13I TCCGGA 1 cut(s) 625
Bsp143I GATC 8 cut(s) 43, 325, 368, 1082, 1450, 1573, 1579, 1715
BspACI CCGC 6 cut(s) 478, 825, 1171, 1365, 1408, 1514
BspANI GGCC 4 cut(s) 54, 189, 566, 1150
BspCNI CTCAG 4 cut(s) 370, 394, 1672, 1906
BspEI TCCGGA 1 cut(s) 625
BspLI GGNNCC 7 cut(s) 45, 66, 133, 146, 188, 1264, 1812
BspMI ACCTGC 1 cut(s) 31
BspOI GCTAGC 2 cut(s) 267, 1137
BspPI GGATC 6 cut(s) 38, 51, 333, 1458, 1574, 1710
BspQI GCTCTTC 1 cut(s) 116
BspT104I TTCGAA 1 cut(s) 496
BsrDI GCAATG 1 cut(s) 1076
BsrFI RCCGGY 1 cut(s) 1480
BsrI ACTGG 6 cut(s) 135, 190, 277, 800, 1636, 1797
BssAI RCCGGY 1 cut(s) 1480
BssECI CCNNGG 3 cut(s) 38, 61, 526
BssMI GATC 8 cut(s) 43, 325, 368, 1082, 1450, 1573, 1579, 1715
BssSI CACGAG 1 cut(s) 1616
BssT1I CCWWGG 1 cut(s) 38
Bst2BI CACGAG 1 cut(s) 1616
Bst2UI CCWGG 8 cut(s) 48, 62, 229, 504, 651, 721, 984, 1362
Bst4CI ACNGT 7 cut(s) 207, 216, 288, 344, 388, 845, 1054
Bst6I CTCTTC 2 cut(s) 116, 1601
BstBAI YACGTR 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 496
BstC8I GCNNGC 5 cut(s) 265, 269, 273, 1135, 1512
BstDEI CTNAG 7 cut(s) 72, 357, 381, 1013, 1250, 1680, 1914
BstDSI CCRYGG 1 cut(s) 526
BstF5I GGATG 9 cut(s) 308, 441, 634, 754, 979, 1333, 1357, 1506, 1657
BstKTI GATC 8 cut(s) 46, 328, 371, 1085, 1453, 1576, 1582, 1718
BstMAI GTCTC 2 cut(s) 443, 1573
BstMBI GATC 8 cut(s) 43, 325, 368, 1082, 1450, 1573, 1579, 1715
BstMWI GCNNNNNNNGC 3 cut(s) 572, 611, 1130
BstNI CCWGG 8 cut(s) 48, 62, 229, 504, 651, 721, 984, 1362
BstNSI RCATGY 1 cut(s) 1711
BstSCI CCNGG 8 cut(s) 46, 60, 227, 502, 649, 719, 982, 1360
BstSFI CTRYAG 1 cut(s) 1086
BstSLI GKGCMC 1 cut(s) 799
BstV2I GAAGAC 4 cut(s) 1055, 1194, 1710, 1840
BstX2I RGATCY 4 cut(s) 43, 368, 1579, 1715
BstXI CCANNNNNNTGG 2 cut(s) 197, 1401
BstYI RGATCY 4 cut(s) 43, 368, 1579, 1715
BsuRI GGCC 4 cut(s) 54, 189, 566, 1150
BtgI CCRYGG 1 cut(s) 526
BtsCI GGATG 9 cut(s) 308, 441, 634, 754, 979, 1333, 1357, 1506, 1657
BtsI GCAGTG 2 cut(s) 133, 1224
BtsIMutI CAGTG 4 cut(s) 133, 850, 1224, 1911
BveI ACCTGC 1 cut(s) 31
Cac8I GCNNGC 5 cut(s) 265, 269, 273, 1135, 1512
Cfr10I RCCGGY 1 cut(s) 1480
Cfr13I GGNCC 7 cut(s) 53, 65, 144, 187, 225, 1550, 1889
CsiI ACCWGGT 1 cut(s) 649
Csp6I GTAC 7 cut(s) 150, 164, 976, 1292, 1629, 1845, 1901
CspAI ACCGGT 1 cut(s) 1480
CspCI CAANNNNNGTGG 2 cut(s) 917, 952
CviAII CATG 7 cut(s) 239, 455, 887, 1142, 1547, 1708, 1807
CviQI GTAC 7 cut(s) 150, 164, 976, 1292, 1629, 1845, 1901
DdeI CTNAG 7 cut(s) 72, 357, 381, 1013, 1250, 1680, 1914
DpnI GATC 8 cut(s) 45, 327, 370, 1084, 1452, 1575, 1581, 1717
DpnII GATC 8 cut(s) 43, 325, 368, 1082, 1450, 1573, 1579, 1715
DraI TTTAAA 1 cut(s) 1389
DraIII CACNNNGTG 1 cut(s) 458
DriI GACNNNNNGTC 2 cut(s) 10, 390
EaeI YGGCCR 1 cut(s) 564
Eam1104I CTCTTC 2 cut(s) 116, 1601
Eam1105I GACNNNNNGTC 2 cut(s) 10, 390
EarI CTCTTC 2 cut(s) 116, 1601
EciI GGCGGA 2 cut(s) 1423, 1529
Eco130I CCWWGG 1 cut(s) 38
Eco47I GGWCC 5 cut(s) 65, 144, 225, 1550, 1889
Eco57I CTGAAG 2 cut(s) 90, 316
Eco88I CYCGRG 1 cut(s) 183
EcoO109I RGGNCCY 1 cut(s) 144
EcoRII CCWGG 8 cut(s) 46, 60, 227, 502, 649, 719, 982, 1360
EcoT14I CCWWGG 1 cut(s) 38
EcoT22I ATGCAT 2 cut(s) 1078, 1359
ErhI CCWWGG 1 cut(s) 38
FaeI CATG 7 cut(s) 242, 458, 890, 1145, 1550, 1711, 1810
FaqI GGGAC 5 cut(s) 330, 353, 926, 1046, 1381
FatI CATG 7 cut(s) 238, 454, 886, 1141, 1546, 1707, 1806
FauI CCCGC 3 cut(s) 818, 1164, 1358
FblI GTMKAC 1 cut(s) 1612
FokI GGATG 9 cut(s) 315, 448, 641, 761, 966, 1340, 1344, 1513, 1644
FspBI CTAG 8 cut(s) 39, 57, 153, 195, 264, 366, 1029, 1134
GsuI CTGGAG 2 cut(s) 645, 1140
HaeIII GGCC 4 cut(s) 54, 189, 566, 1150
HapII CCGG 3 cut(s) 512, 626, 1481
Hin1II CATG 7 cut(s) 242, 458, 890, 1145, 1550, 1711, 1810
HincII GTYRAC 1 cut(s) 1486
HindII GTYRAC 1 cut(s) 1486
HindIII AAGCTT 1 cut(s) 581
HinfI GANTC 8 cut(s) 11, 17, 177, 355, 1059, 1153, 1455, 1609
HpaI GTTAAC 1 cut(s) 1486
HpaII CCGG 3 cut(s) 512, 626, 1481
HphI GGTGA 7 cut(s) 12, 26, 511, 542, 1450, 1453, 1582
Hpy166II GTNNAC 6 cut(s) 200, 656, 1292, 1486, 1613, 1847
Hpy188I TCNGA 6 cut(s) 16, 294, 384, 748, 1573, 1681
Hpy8I GTNNAC 6 cut(s) 200, 656, 1292, 1486, 1613, 1847
HpyAV CCTTC 3 cut(s) 532, 981, 1106
HpyCH4III ACNGT 7 cut(s) 207, 216, 288, 344, 388, 845, 1054
HpyCH4IV ACGT 2 cut(s) 166, 784
HpyCH4V TGCA 8 cut(s) 461, 883, 966, 1010, 1076, 1357, 1430, 1729
HpyF10VI GCNNNNNNNGC 3 cut(s) 572, 611, 1130
HpyF3I CTNAG 7 cut(s) 72, 357, 381, 1013, 1250, 1680, 1914
HpySE526I ACGT 2 cut(s) 166, 784
Hsp92II CATG 7 cut(s) 242, 458, 890, 1145, 1550, 1711, 1810
Kpn2I TCCGGA 1 cut(s) 625
KspAI GTTAAC 1 cut(s) 1486
Kzo9I GATC 8 cut(s) 43, 325, 368, 1082, 1450, 1573, 1579, 1715
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 5 cut(s) 362, 699, 1121, 1262, 1816
LweI GCATC 4 cut(s) 838, 870, 997, 1366
MabI ACCWGGT 1 cut(s) 649
MaeI CTAG 8 cut(s) 39, 57, 153, 195, 264, 366, 1029, 1134
MaeII ACGT 2 cut(s) 166, 784
MaeIII GTNAC 1 cut(s) 167
MalI GATC 8 cut(s) 45, 327, 370, 1084, 1452, 1575, 1581, 1717
MboI GATC 8 cut(s) 43, 325, 368, 1082, 1450, 1573, 1579, 1715
MboII GAAGA 9 cut(s) 96, 133, 1060, 1121, 1199, 1292, 1618, 1715, 1845
MfeI CAATTG 2 cut(s) 969, 1104
MflI RGATCY 4 cut(s) 43, 368, 1579, 1715
MhlI GDGCHC 1 cut(s) 799
MlyI GAGTC 6 cut(s) 11, 20, 349, 1068, 1449, 1618
MmeI TCCRAC 2 cut(s) 726, 1838
Mph1103I ATGCAT 2 cut(s) 1078, 1359
MroI TCCGGA 1 cut(s) 625
MslI CAYNNNNRTG 1 cut(s) 1852
MspI CCGG 3 cut(s) 512, 626, 1481
MspR9I CCNGG 8 cut(s) 48, 62, 229, 504, 651, 721, 984, 1362
MunI CAATTG 2 cut(s) 969, 1104
MvaI CCWGG 8 cut(s) 48, 62, 229, 504, 651, 721, 984, 1362
MwoI GCNNNNNNNGC 3 cut(s) 572, 611, 1130
NdeII GATC 8 cut(s) 43, 325, 368, 1082, 1450, 1573, 1579, 1715
NheI GCTAGC 2 cut(s) 263, 1133
NlaIII CATG 7 cut(s) 242, 458, 890, 1145, 1550, 1711, 1810
NlaIV GGNNCC 7 cut(s) 45, 66, 133, 146, 188, 1264, 1812
NmuCI GTSAC 1 cut(s) 167
NsiI ATGCAT 2 cut(s) 1078, 1359
NspI RCATGY 1 cut(s) 1711
NspV TTCGAA 1 cut(s) 496
PaqCI CACCTGC 1 cut(s) 31
PciI ACATGT 1 cut(s) 1707
PciSI GCTCTTC 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 1582
PfeI GAWTC 2 cut(s) 177, 1153
PflFI GACNNNGTC 1 cut(s) 1839
PfoI TCCNGGA 3 cut(s) 227, 502, 982
PinAI ACCGGT 1 cut(s) 1480
PleI GAGTC 6 cut(s) 11, 19, 349, 1067, 1449, 1617
PpsI GAGTC 6 cut(s) 11, 19, 349, 1067, 1449, 1617
Ppu21I YACGTR 1 cut(s) 167
PpuMI RGGWCCY 1 cut(s) 144
PscI ACATGT 1 cut(s) 1707
Psp5II RGGWCCY 1 cut(s) 144
Psp6I CCWGG 8 cut(s) 46, 60, 227, 502, 649, 719, 982, 1360
PspGI CCWGG 8 cut(s) 46, 60, 227, 502, 649, 719, 982, 1360
PspN4I GGNNCC 7 cut(s) 45, 66, 133, 146, 188, 1264, 1812
PspPI GGNCC 7 cut(s) 53, 65, 144, 187, 225, 1550, 1889
PspPPI RGGWCCY 1 cut(s) 144
PsuI RGATCY 4 cut(s) 43, 368, 1579, 1715
PsyI GACNNNGTC 1 cut(s) 1839
RsaI GTAC 7 cut(s) 151, 165, 977, 1293, 1630, 1846, 1902
RsaNI GTAC 7 cut(s) 150, 164, 976, 1292, 1629, 1845, 1901
RseI CAYNNNNRTG 1 cut(s) 1852
SapI GCTCTTC 1 cut(s) 116
Sau3AI GATC 8 cut(s) 43, 325, 368, 1082, 1450, 1573, 1579, 1715
Sau96I GGNCC 7 cut(s) 53, 65, 144, 187, 225, 1550, 1889
SchI GAGTC 6 cut(s) 11, 20, 349, 1068, 1449, 1618
ScrFI CCNGG 8 cut(s) 48, 62, 229, 504, 651, 721, 984, 1362
SduI GDGCHC 1 cut(s) 799
SexAI ACCWGGT 1 cut(s) 649
SfaNI GCATC 4 cut(s) 838, 870, 997, 1366
SfcI CTRYAG 1 cut(s) 1086
SfuI TTCGAA 1 cut(s) 496
SinI GGWCC 5 cut(s) 65, 144, 225, 1550, 1889
SmiMI CAYNNNNRTG 1 cut(s) 1852
SmlI CTYRAG 1 cut(s) 853
SmoI CTYRAG 1 cut(s) 853
SsiI CCGC 6 cut(s) 478, 825, 1171, 1365, 1408, 1514
SspI AATATT 2 cut(s) 1196, 1435
SspMI CTAG 8 cut(s) 39, 57, 153, 195, 264, 366, 1029, 1134
StyD4I CCNGG 8 cut(s) 46, 60, 227, 502, 649, 719, 982, 1360
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 7 cut(s) 207, 216, 288, 344, 388, 845, 1054
TaiI ACGT 2 cut(s) 169, 787
TaqI TCGA 7 cut(s) 140, 248, 496, 1057, 1381, 1453, 1576
TfiI GAWTC 2 cut(s) 177, 1153
TscAI CASTG 4 cut(s) 133, 850, 1224, 1918
TseFI GTSAC 1 cut(s) 167
Tsp45I GTSAC 1 cut(s) 167
TspDTI ATGAA 9 cut(s) 602, 657, 1200, 1430, 1517, 1535, 1545, 1646, 1878
TspGWI ACGGA 2 cut(s) 515, 1918
TspRI CASTG 4 cut(s) 133, 850, 1224, 1918
Tth111I GACNNNGTC 1 cut(s) 1839
VpaK11BI GGWCC 5 cut(s) 65, 144, 225, 1550, 1889
XapI RAATTY 4 cut(s) 734, 919, 1320, 1598
XceI RCATGY 1 cut(s) 1711
XcmI CCANNNNNNNNNTGG 2 cut(s) 1401, 1804
XmaJI CCTAGG 1 cut(s) 38
XmiI GTMKAC 1 cut(s) 1612
XspI CTAG 8 cut(s) 39, 57, 153, 195, 264, 366, 1029, 1134
Zsp2I ATGCAT 2 cut(s) 1078, 1359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.