FvH4_4g13341

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000094
Physical Location & Seq
Forward (+)
16960965 .. 16965530
4566 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13341.t1

Sequence Viewer

Length: 4566 bp
ATGCCTCCTAGGCGCCCACGGCAACCCCGTGTGGCTACTTCGACCAATGCCGATGAAGGCAATGAGGTCAGGGGACTGGTGGATGATATTGGAAATATGCTGAGGGATGTCATGGATCGGGCTCCACGCCCTCCTACTGTGAAAAGGAGAGAAGTTTTTGAAGCTGGAGCCAGAGAGTTCAAGGGTGCCAAGGGACCCCTTGATGCTTATACTTGGGTGGACAAAATGGAAAAAGCTTTTGCCAGCACAGAGTTGCCAGAAGAAAAGAAGGTGATGATGGCAGTAACTTTCCTGGATGATTCAGCACATCACTGGTGGTCGCTTGCCAGCAGGAATGTTGAGGATGCGCTGAATATGTCGTGGGAACAGTTTAAGACTCTCTTCTTGGGGCAGTATTTCAGTCCTGCCCACCGCAGCAGAATTCAGTCAGATTTCCTGAATATGAAGAAGGGGGATGATGAAGGCGTTATAGAGTTCCAGGAGAGATTCACTTCCCTGAGCTATCATGTGTCATATCTGGTTCCTGATGAGAGGACCAGGATGGAGATGTTCATTGGGGCTCTTGGTGGAGTGTTTGCAGATAAGATGACGGGTGTTTATTACCCGACTTTCTCTGATGCTGTTCATGCAGCCATGGCCATTGAGTCTATGGGGATTTATAGTGCTCGGCCACGAGATTTCGGTGGTCCCAGTCAGGGTCCATCCAAGAAAGCTGCTTCATCATCCGCTTCAGGTTCATCAGCTGGTAGTGGCAGTAGTGGGGGATCAGGTTCTCATTCTCGGTTCAGGGTTAGAGGACGTCACAGGAGACCTGCTAGGAGCCAGTTGGGTAGGCAGCAGTCTGGGCAGTATGGTTCGGGTGGCAGTTTCCATGGTGGGTCATCCGGTAGCCAGACTTTTTCATATGGGCAGCAGCAGCAGTTTTCTGGGTGCTTTGAGTGTGGTGGTCAGGACCACTTCAGGAGAGATTGCCCATTGTTGGCTCAGAGGACCACTCCCACCCCTACTCAGCCTATTGGTCAGTCTTCAGCTGGAGGCTCTAGTAGTGGTGCCCAGGGCAGTGCTTCGGTCAGAGGTGGCTCCCAACGGGGAAGTGGTCAGCGTGGGCGTCCCATGACTCGCGCTAGACTGCATGCTATGACCCAGCAGGAGGGTCGTACTTCTCCAGATGTTATCATTGGTACGTTATTGATTTTTGGTCAGCCTGCTTTTACTTTAATTGATCCCGGAGCCACGCATTCATTCATGTCTAGTAGATTTGCCCTCCATGCCAATGTGCCATCCTCGTCTCTTCCAGGCGAGTGGCATGTGTCTCTACCCTCCGGTGAGGTGATGAGAATAAATTGGGTATTTAGTGGCTGTGAAGTGGTAGTGGAAGGAGTTAATTTCGAGGCTGATTTAATTCCTTTAGATATAGTGGAGTTTGATGTGATCTTGGGAATGGACTTTTTGGAAGCATATAGAGCTATGGTAGATTGTTTTCAAAAAGTAGTGGTGCTTCGAAGTCCAGATGGTTTTGAGGTTGCTTTCCAAGGGGAAAGAGATGTTATTTTTAGTTGCATAATTTCCGCTGTTGCTGCAAGGAAATTGTTGAGTAAAGGCTGTCAGGCATTTTTAGCTCATGTGGTGGATACAAGTATGGGAGTTGTGGGATTAGAAGATATTCCTGTAGTAAGAGAGTTTCCGGATGTTTTTCCTGAGGAGTTGCCTGGTTTGCCTCCTGCAAGGGAGATAGACTTCTCTATAGAATTACTTCCCGGAACTATGCCTATCTCTCAGGCGCCTTACAGAATGGCTCCAGCGGAATTGAAAGAGTTGAAGACTCAATTGCAGGAGTTGGTAGATAAGGGTTTTATTCGGCCTAGCATGTCTCCATGGGGTGCTCCGGTGTTGTTTGTTAAGAAGAAAGATGGCACCATGAGACTATGCATTGATTATAGGAAGCTGAATCAGGTCACAGTGAAGAATAAATATCCGTTGCCTCGGATCGATGATTTATTTGATCAGTTGAGGGGTGCCTCTGTTTTCTCCAAGATTGATTTGAGATCGGGGTATCATCAGTTGCGAATCCGAGAGGGGGACATAGCCAAGACTGCTTTTCGGTCACGATATGGTCATTATGAATTTGTGGTGATGCCTTTTGGACTTACTAATGCACCCGCGGCGTTCATGGATCTCATGAATAAGGTGTTTAGTCCATATCTTGATCGTTTCGTGATCGTATTCATAGATGATATCTTGGTTTACTCAAGAAGTGAGAAAGAGCATGCCAAGCATCTAAGAATAATATTGCGGACCTTGGAAAAGTCGCAGTTATTTGCTAAGTTTAGCAAGTGTGAGTTTTGGCTTGATAAAGTGGGGTTCTTGGGTCATGTGATTTCGGCAGAAGGAGTTAGTGTGGATCCCCAAAAGGTGGAAGCAGTGTCGAATTGGAGAAGACCCACCACTGTGACGGAGATTCGTAGTTTCTTGGGATTAGCAGGTTATTATCGGCGCTTTGTGAAAGATTTTTCAAAGATTGCTGCTCCTCTCACAAAGTTGACCAGAAAGGGGGTTAAGTTTGAATGGTCAGATCAGTGCGAGCAAAGTTTTCAAGAATTGAAGAATTGCTTGACAAGTGCTCCTGTTTTGGCACTTCCGGATGATAGTGGAGATTATGTGGTCTATTGTGATGCTTCTAGACGTGGTCTAGGTGGAGTTTTGATGCAGCATGATCGAGTTATTGCCTACGCTTCTAGGCAATTGAAGCCGCATGAGGGGAATTATCCTACTCATGATCTGGAGCTTGCAGCGGTAGTTTTTGCTCTCAAGTTGTGGAGACACTATCTATATGGAGCCAAGTGTCAGATATTTACCGATCACAAGAGTCTTCGGTATGTGTTCACTCAAAGAGAGCTTAATATGAGACAGAGAAGATGGATGGAGTTAATCAGTGATTATGATTGCACTATTGAGTATCATCCTGGAAAAGCTAATGTGGTAGCTGATGCTCTTAGTCGAAATCCCTCCATATCTTTATCTCATATAAAGATGGTTCGTGTCCCGCTTCTTTGTGGGTTGCGAGCTACAGGAGTGGATTTGTCAGTGGATGAGGTTGGTGCTTTGATAGCCAGTTTTCATGTGAGGCCAGTTTTGGTTGATAGAGTGCGGGAAGCACAGTATAATGATCCTTCCATAGTTCAATTGATGGAAAGAGCTTCGAATGGAGCCGCAGCCGATCATTTTTCAGTTAGAAGAGATGGAACTCTATTGTTTAAAAATCGGCTATGTGTTCCAAAAAGAAATGAAGAGTTGAAGAGTGAGATTATGGAGGAAGCTCATAGCTCTGCATATGCTGCTCATCCGGGTAGCACAAAAATGTATCGGATTTTGAAGGATTATTATTGGTGGCCAAACATGAAGAGGGAAATTGCAGCTTATGTGAGTAGATGTCTGATTTGTCAGCAAGTCAAGGCTGAAAGGCAGAAGCCGTCAGGATTGCTTGAGCCGTTGCCCATTCCAGTGTGGAAGTGGGACCACATCACCATGGATTTTGTTTTTAGCTTACCTCGTACCCGTGATGGGCATGACGGTATTTGGGTGATTGTGGATAGACTCACCAAGTCTGCACATTTCTTGCCAGTGGGAAAGAAGTATAAATTGGATAAATTGGCGGAGCTCTATGTGAATGAAATTGTGAGGCTTCATGGTGTTCCTGAATCAATAGTGTCAGATCGGGACCCTCGATTTGCTTCTAAATTCTGGGGAGCTCTTCAGGCAGCTTTAGGTACCCAATTACTCTTCAGCACTGCTTTTCATCCTCAGACTGATGGGCAGTCCGAGAGGACTATTCAGACTTTGGAGGATATGTTGAGGGCTTGTGCCATGCAGTTCAGAGGTAGTTGGGATAAAAACCTATCTTTGATGGAATTTGCCTATAATAATAGCTATCATTCTAGCATTGGTATGGCGCCCTTTGAGGCTCTTTATGGGAGGCAGTGTCGCACGCCTTTGTGTTGGAATGAAGTAGGGGAAAAAGAGCTTTGTGGCCCAGAGTTAATCCAGGTTACGAATGAGAAGATTAAAGTGGTTAGAGATAGACTCAAGACCGCTCAGAGTAGGCAGAAGAGTTATGCAGATGTCCGTAGGAGAGATCTTGAATTTCAAGTGGGTGATTGGGTGTTCCTGAAATTATCTCCTTGGAAAGGGGTTGTGCGTTTCGGTAAGCGTGGGAAGCTTAGTCCGAGGTATATTGACCCTTATGAGATTATAGAGCGAGTTGGCTCACTTGCTTATCGATTAGCCTTGCCTCCAGAGTTATCCCGGATACATAATGTGTTTCATGTATCCATGCTTCGCAAATATATTGCCGATCCTTCTCATGTGCTGCAAGAGCAGCCGATCAGTTTGACGAGAGATTTGACCTATGAAGAGGAACCGGTTCAGATTCTCGACCGAAAGGAGCAAGTGCTTAGGAATAAGACAATCCCGCTTGTCAAGGTGCTGTGGAGAAGTCATCAAGTAGAAGAAGCTACTTGGGAATCAGAGGAGCAAATGATGCAGCAGTATCCGTATCTCTTCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve zf-CCHC

Protein Analysis

1522

Amino Acids

171.89

Weight (kDa)

8.93

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 93 - 187 2.4e-14 Retrotransposon gag protein
RVP_2 PF08284 372 - 502 1.8e-33 Retroviral aspartyl protease
RVT_1 PF00078 633 - 793 6.1e-36 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 856 - 950 8e-32 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 883 - 980 1.7e-32 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 1095 - 1152 4.3e-20 Integrase zinc binding domain
SH3_Tf2-1 PF24626 1384 - 1448 8.4e-27 Tf2-1-like, SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000094)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42190
fragaria_vesca FvH4_1g22371 FvH4_1g23261 FvH4_1g27933 FvH4_1g27934 FvH4_1g29231 FvH4_2g02781 FvH4_2g08031 FvH4_2g08581 FvH4_3g06961 FvH4_3g06962 FvH4_3g13531 FvH4_3g14391 FvH4_3g14392 FvH4_3g18622 FvH4_3g18964 FvH4_3g26314 FvH4_3g28781 FvH4_3g30771 FvH4_3g30772 FvH4_3g31752 FvH4_3g32803 FvH4_3g35751 FvH4_3g35752 FvH4_4g02151 FvH4_4g02642 FvH4_4g05523 FvH4_4g05524 FvH4_4g06574 FvH4_4g06722 FvH4_4g08644 FvH4_4g08645 FvH4_4g08646 FvH4_4g08851 FvH4_4g08971 FvH4_4g09081 FvH4_4g09243 FvH4_4g09244 FvH4_4g09283 FvH4_4g10021 FvH4_4g10032 FvH4_4g10033 FvH4_4g10182 FvH4_4g10361 FvH4_4g12502 FvH4_4g12503 FvH4_4g13341 FvH4_4g13942 FvH4_4g13943 FvH4_4g13944 FvH4_4g14432 FvH4_4g14822 FvH4_4g14823 FvH4_4g15291 FvH4_4g15292 FvH4_4g15293 FvH4_4g15471 FvH4_4g15583 FvH4_4g15584 FvH4_4g15585 FvH4_4g15586 FvH4_4g15587 FvH4_4g15591 FvH4_4g15592 FvH4_4g15593 FvH4_4g16179 FvH4_4g18131 FvH4_4g21031 FvH4_4g22021 FvH4_4g22021 FvH4_4g22042 FvH4_5g03402 FvH4_5g07411 FvH4_5g13881 FvH4_5g15792 FvH4_5g24682 FvH4_5g24911 FvH4_5g25322 FvH4_5g25641 FvH4_5g36692 FvH4_5g38072 FvH4_5g38171 FvH4_6g05202 FvH4_6g08531 FvH4_6g16342 FvH4_6g21771 FvH4_6g23303 FvH4_6g23961 FvH4_6g25381 FvH4_6g27021 FvH4_6g27022 FvH4_6g30091 FvH4_6g30092 FvH4_7g02051 FvH4_7g06031 FvH4_7g07031 FvH4_7g07581 FvH4_7g08381 FvH4_7g11551 FvH4_7g19711 FvH4_7g25621 FvH4_7g29722
malus_domestica MD06G1134500.v1.1 MD09G1067600.v1.1 MD13G1145500.v1.1 MD15G1166500.v1.1 MD16G1270000.v1.1
prunus_persica Prupe.7G030900_v2.0.a1
pyrus_communis pycom01g00710 pycom01g03760 pycom03g10770 pycom04g02180 pycom05g08050 pycom06g04140 pycom06g11110 pycom07g07550 pycom07g21570 pycom08g17240 pycom09g13900 pycom09g19570 pycom10g22300 pycom11g03210 pycom11g13750 pycom11g24890 pycom12561g00270 pycom12668g00020 pycom12g11280 pycom12g20720 pycom12g20730 pycom13g21410 pycom13g21660 pycom14g06010 pycom14g06370 pycom14g12800 pycom16g21440 pycom16g22530 pycom17g02030 pycom420g00170 pycom50g00020 pycom5153684g00110 pycom520g00860 pycom581g00060
rosa_chinensis RchiOBHm_Chr0c08g0498091 RchiOBHm_Chr0c08g0498101 RchiOBHm_Chr1g0326991 RchiOBHm_Chr2g0084311 RchiOBHm_Chr2g0090701 RchiOBHm_Chr2g0098591 RchiOBHm_Chr2g0109491 RchiOBHm_Chr2g0109501 RchiOBHm_Chr2g0130801 RchiOBHm_Chr2g0145931 RchiOBHm_Chr2g0168091 RchiOBHm_Chr3g0457081 RchiOBHm_Chr3g0457091 RchiOBHm_Chr3g0467781 RchiOBHm_Chr3g0467791 RchiOBHm_Chr3g0493771 RchiOBHm_Chr3g0494051 RchiOBHm_Chr3g0496881 RchiOBHm_Chr4g0401391 RchiOBHm_Chr4g0414861 RchiOBHm_Chr4g0424081 RchiOBHm_Chr5g0009611 RchiOBHm_Chr5g0049881 RchiOBHm_Chr5g0050351 RchiOBHm_Chr5g0053791 RchiOBHm_Chr5g0078031 RchiOBHm_Chr6g0256481 RchiOBHm_Chr6g0271231 RchiOBHm_Chr6g0286381 RchiOBHm_Chr7g0197101 RchiOBHm_Chr7g0221891 RchiOBHm_Chr7g0237801 RchiOBHm_Chr7g0237821
rosa_multiflora Rmu_sc0000509.1_g000034 Rmu_sc0004751.1_g000014 Rmu_sc0008546.1_g000002 Rmu_sc0009320.1_g000003 Rmu_sc0010661.1_g000006 Rmu_sc0029652.1_g000002
rosa_roxburghii Rroxscaffold_10G00433150 Rroxscaffold_15G00447630 Rroxscaffold_26G00447790 Rroxscaffold_29G00441810 Rroxscaffold_32G00442860 Rroxscaffold_33G00439800 Rroxscaffold_34G00443230 Rroxscaffold_3G00220560 Rroxscaffold_3G00227370 Rroxscaffold_3G00239080 Rroxscaffold_3G00239090 Rroxscaffold_3G00273190 Rroxscaffold_4G00323530 Rroxscaffold_4G00326840 Rroxscaffold_57G00443110 Rroxscaffold_5G00338590 Rroxscaffold_5G00347530 Rroxscaffold_68G00446990 Rroxscaffold_76G00448570 Rroxscaffold_90G00448860
rosa_rugosa Rorug06G0233600
rosa_samantha Rh3CG302700 Rh6DG286700
rosa_wichuraiana Rw0G002280 Rw0G004080 Rw0G011830 Rw1G003690 Rw1G008800 Rw1G009130 Rw1G009140 Rw1G009150 Rw1G009160 Rw1G009410 Rw1G009580 Rw1G012770 Rw1G015850 Rw1G026860 Rw2G003780 Rw2G006480 Rw2G016520 Rw2G016960 Rw2G018170 Rw2G025180 Rw2G025510 Rw2G025610 Rw2G025750 Rw2G025920 Rw2G029950 Rw2G046360 Rw2G046370 Rw2G046380 Rw3G009070 Rw3G025820 Rw3G027340 Rw3G027900 Rw3G028150 Rw3G029060 Rw4G002440 Rw4G005960 Rw4G007520 Rw4G011970 Rw4G011980 Rw4G012190 Rw4G012530 Rw4G018490 Rw4G026380 Rw5G003970 Rw5G030920 Rw5G031780 Rw5G031900 Rw5G033030 Rw5G033040 Rw5G036330 Rw5G039730 Rw5G048110 Rw6G006670 Rw6G007910 Rw6G009720 Rw6G010990 Rw6G012930 Rw6G012940 Rw6G013420 Rw6G014690 Rw6G015290 Rw6G015790 Rw6G015920 Rw6G036510 Rw7G016650 Rw7G023140 Rw7G028650 Rw7G036450 Rw7G036460 Rw7G036910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 802
Acc36I ACCTGC 2 cut(s) 820, 2471
Acc65I GGTACC 1 cut(s) 3767
AccB1I GGYRCC 8 cut(s) 12, 185, 1049, 1780, 1913, 2015, 3767, 3949
AccB7I CCANNNNNTGG 1 cut(s) 2413
AccBSI CCGCTC 1 cut(s) 4091
AccII CGCG 2 cut(s) 1122, 2162
AccIII TCCGGA 2 cut(s) 1684, 2638
AclWI GGATC 9 cut(s) 123, 772, 1217, 1994, 2181, 2396, 2409, 3161, 4346
AcoI YGGCCR 3 cut(s) 636, 668, 3389
AcsI RAATTY 5 cut(s) 420, 2123, 3737, 3908, 4139
AcuI CTGAAG 6 cut(s) 714, 943, 1011, 3737, 3766, 4543
AcyI GRCGYC 5 cut(s) 13, 799, 1108, 1781, 3950
AfaI GTAC 4 cut(s) 1159, 1183, 3553, 3769
AfiI CCNNNNNNNGG 9 cut(s) 695, 979, 1150, 2077, 2413, 2550, 2756, 3561, 4184
AgeI ACCGGT 1 cut(s) 4417
AjiI CACGTC 1 cut(s) 2684
AjnI CCWGG 8 cut(s) 291, 477, 536, 1053, 1294, 1708, 2962, 4041
AjuI GAANNNNNNNTTGG 2 cut(s) 3623, 3655
AleI CACNNNNGTG 1 cut(s) 2447
Alw21I GWGCWC 5 cut(s) 667, 1885, 2623, 3660, 3751
Alw26I GTCTC 7 cut(s) 802, 1293, 1317, 1875, 1915, 2812, 2899
AlwI GGATC 9 cut(s) 123, 772, 1217, 1994, 2181, 2396, 2409, 3161, 4346
Aor13HI TCCGGA 2 cut(s) 1684, 2638
AoxI GGCC 6 cut(s) 636, 668, 1859, 3125, 3389, 4027
ApoI RAATTY 5 cut(s) 420, 2123, 3737, 3908, 4139
ArsI GACNNNNNNTTYG 4 cut(s) 3710, 3742, 4381, 4413
AsiGI ACCGGT 1 cut(s) 4417
Asp700I GAANNNNTTC 3 cut(s) 1662, 1752, 4419
Asp718I GGTACC 1 cut(s) 3767
AspA2I CCTAGG 1 cut(s) 8
AspLEI GCGC 6 cut(s) 15, 349, 1124, 1783, 2496, 3952
AsuC2I CCSGG 4 cut(s) 1227, 1758, 3345, 4303
AsuHPI GGTGA 8 cut(s) 283, 1337, 1342, 2143, 3514, 3589, 3592, 4163
AsuII TTCGAA 2 cut(s) 1501, 3200
AvaII GGWCC 9 cut(s) 194, 534, 686, 698, 952, 990, 2295, 3514, 3718
AvrII CCTAGG 1 cut(s) 8
AxyI CCTNAGG 1 cut(s) 1698
BaeGI GKGCMC 1 cut(s) 1054
BalI TGGCCA 2 cut(s) 638, 3391
BamHI GGATCC 1 cut(s) 2401
BanI GGYRCC 8 cut(s) 12, 185, 1049, 1780, 1913, 2015, 3767, 3949
BanII GRGCYC 4 cut(s) 124, 562, 3660, 3751
BarI GAAGNNNNNNTAC 4 cut(s) 1482, 1514, 4317, 4349
BauI CACGAG 1 cut(s) 672
BbsI GAAGAC 4 cut(s) 1017, 1826, 2443, 2861
Bbv12I GWGCWC 5 cut(s) 667, 1885, 2623, 3660, 3751
BbvCI CCTCAGC 1 cut(s) 101
BceAI ACGGC 3 cut(s) 35, 3454, 3472
BcgI CGANNNNNNTGC 4 cut(s) 1136, 1170, 2696, 2730
BciT130I CCWGG 8 cut(s) 293, 479, 538, 1055, 1296, 1710, 2964, 4043
BciVI GTATCC 4 cut(s) 1624, 4299, 4336, 4557
BclI TGATCA 1 cut(s) 2002
BcnI CCSGG 4 cut(s) 1227, 1758, 3345, 4303
BcoDI GTCTC 7 cut(s) 802, 1293, 1317, 1875, 1915, 2812, 2899
BfmI CTRYAG 3 cut(s) 1668, 1743, 3066
BfoI RGCGCY 4 cut(s) 16, 1784, 2497, 3953
BfuAI ACCTGC 2 cut(s) 820, 2471
BfuI GTATCC 4 cut(s) 1624, 4299, 4336, 4557
BglI GCCNNNNNGGC 1 cut(s) 10
BglII AGATCT 1 cut(s) 4132
BlnI CCTAGG 1 cut(s) 8
Bme18I GGWCC 9 cut(s) 194, 534, 686, 698, 952, 990, 2295, 3514, 3718
BmgBI CACGTC 1 cut(s) 2684
BmrI ACTGGG 1 cut(s) 684
BmsI GCATC 9 cut(s) 193, 334, 607, 2124, 2284, 2662, 2694, 2977, 4527
BmuI ACTGGG 1 cut(s) 684
BpiI GAAGAC 4 cut(s) 1017, 1826, 2443, 2861
BplI GAGNNNNNCTC 4 cut(s) 979, 1011, 4065, 4097
BpmI CTGGAG 6 cut(s) 186, 1053, 1149, 1782, 2801, 4275
Bpu10I CCTNAGC 3 cut(s) 101, 497, 4451
Bpu14I TTCGAA 2 cut(s) 1501, 3200
BpuEI CTTGAG 4 cut(s) 2233, 2792, 3503, 4067
BpuMI CCSGG 4 cut(s) 1227, 1758, 3345, 4303
Bsa29I ATCGAT 2 cut(s) 1989, 4276
BsaHI GRCGYC 5 cut(s) 13, 799, 1108, 1781, 3950
BsaI GGTCTC 1 cut(s) 802
BsaWI WCCGGW 6 cut(s) 884, 1322, 1684, 1885, 2638, 4417
Bsc4I CCNNNNNNNGG 9 cut(s) 695, 979, 1150, 2077, 2413, 2550, 2756, 3561, 4184
Bse118I RCCGGY 1 cut(s) 4417
Bse1I ACTGG 8 cut(s) 81, 317, 690, 823, 3111, 3128, 3500, 3620
Bse21I CCTNAGG 1 cut(s) 1698
Bse3DI GCAATG 1 cut(s) 67
BseAI TCCGGA 2 cut(s) 1684, 2638
BseBI CCWGG 8 cut(s) 293, 479, 538, 1055, 1296, 1710, 2964, 4043
BseCI ATCGAT 2 cut(s) 1989, 4276
BseLI CCNNNNNNNGG 9 cut(s) 695, 979, 1150, 2077, 2413, 2550, 2756, 3561, 4184
BseMI GCAATG 1 cut(s) 67
BseMII CTCAG 8 cut(s) 92, 488, 998, 1022, 1689, 1790, 3815, 4106
BseNI ACTGG 8 cut(s) 81, 317, 690, 823, 3111, 3128, 3500, 3620
BseRI GAGGAG 3 cut(s) 1715, 2517, 4541
BseSI GKGCMC 1 cut(s) 1054
BseYI CCCAGC 1 cut(s) 1143
BsgI GTGCAG 1 cut(s) 3591
Bsh1236I CGCG 2 cut(s) 1122, 2162
Bsh1285I CGRYCG 1 cut(s) 4435
BshFI GGCC 6 cut(s) 638, 670, 1861, 3127, 3391, 4029
BshNI GGYRCC 8 cut(s) 12, 185, 1049, 1780, 1913, 2015, 3767, 3949
BshTI ACCGGT 1 cut(s) 4417
BshVI ATCGAT 2 cut(s) 1989, 4276
BsiEI CGRYCG 1 cut(s) 4435
BsiHKAI GWGCWC 5 cut(s) 667, 1885, 2623, 3660, 3751
BslFI GGGAC 8 cut(s) 87, 207, 672, 1095, 2093, 3026, 3527, 3731
BslI CCNNNNNNNGG 9 cut(s) 695, 979, 1150, 2077, 2413, 2550, 2756, 3561, 4184
BsmAI GTCTC 7 cut(s) 802, 1293, 1317, 1875, 1915, 2812, 2899
BsmBI CGTCTC 1 cut(s) 1293
BsmFI GGGAC 8 cut(s) 87, 207, 672, 1095, 2093, 3026, 3527, 3731
BsmI GAATGC 1 cut(s) 1237
BsnI GGCC 6 cut(s) 638, 670, 1861, 3127, 3391, 4029
Bso31I GGTCTC 1 cut(s) 802
Bsp119I TTCGAA 2 cut(s) 1501, 3200
Bsp1286I GDGCHC 8 cut(s) 124, 562, 667, 1054, 1885, 2623, 3660, 3751
Bsp13I TCCGGA 2 cut(s) 1684, 2638
Bsp19I CCATGG 4 cut(s) 633, 871, 1874, 3525
BspANI GGCC 6 cut(s) 638, 670, 1861, 3127, 3391, 4029
BspCNI CTCAG 8 cut(s) 93, 489, 997, 1021, 1690, 1789, 3814, 4105
BspDI ATCGAT 2 cut(s) 1989, 4276
BspEI TCCGGA 2 cut(s) 1684, 2638
BspFNI CGCG 2 cut(s) 1122, 2162
BspHI TCATGA 2 cut(s) 2178, 2773
BspMI ACCTGC 2 cut(s) 820, 2471
BspPI GGATC 9 cut(s) 123, 772, 1217, 1994, 2181, 2396, 2409, 3161, 4346
BspQI GCTCTTC 1 cut(s) 3756
BspT104I TTCGAA 2 cut(s) 1501, 3200
BspT107I GGYRCC 8 cut(s) 12, 185, 1049, 1780, 1913, 2015, 3767, 3949
BspTNI GGTCTC 1 cut(s) 802
BsrBI CCGCTC 1 cut(s) 4091
BsrDI GCAATG 1 cut(s) 67
BsrFI RCCGGY 1 cut(s) 4417
BsrI ACTGG 8 cut(s) 81, 317, 690, 823, 3111, 3128, 3500, 3620
BssAI RCCGGY 1 cut(s) 4417
BssNI GRCGYC 5 cut(s) 13, 799, 1108, 1781, 3950
BssSI CACGAG 1 cut(s) 672
BssT1I CCWWGG 9 cut(s) 8, 189, 633, 871, 1531, 1874, 2298, 3525, 4178
Bst2BI CACGAG 1 cut(s) 672
Bst2UI CCWGG 8 cut(s) 293, 479, 538, 1055, 1296, 1710, 2964, 4043
Bst4CI ACNGT 6 cut(s) 139, 369, 1960, 2449, 3159, 3572
BstACI GRCGYC 5 cut(s) 13, 799, 1108, 1781, 3950
BstAPI GCANNNNNTGC 2 cut(s) 3335, 4537
BstBI TTCGAA 2 cut(s) 1501, 3200
BstDSI CCRYGG 6 cut(s) 17, 633, 871, 1874, 2160, 3525
BstENI CCTNNNNNAGG 1 cut(s) 4182
BstFNI CGCG 2 cut(s) 1122, 2162
BstH2I RGCGCY 4 cut(s) 16, 1784, 2497, 3953
BstHHI GCGC 6 cut(s) 15, 349, 1124, 1783, 2496, 3952
BstMAI GTCTC 7 cut(s) 802, 1293, 1317, 1875, 1915, 2812, 2899
BstMCI CGRYCG 1 cut(s) 4435
BstNI CCWGG 8 cut(s) 293, 479, 538, 1055, 1296, 1710, 2964, 4043
BstNSI RCATGY 4 cut(s) 1136, 1310, 1870, 2270
BstSFI CTRYAG 3 cut(s) 1668, 1743, 3066
BstSLI GKGCMC 1 cut(s) 1054
BstUI CGCG 2 cut(s) 1122, 2162
BstV2I GAAGAC 4 cut(s) 1017, 1826, 2443, 2861
BstX2I RGATCY 3 cut(s) 2173, 2401, 4132
BstXI CCANNNNNNTGG 1 cut(s) 1302
BstYI RGATCY 3 cut(s) 2173, 2401, 4132
Bsu15I ATCGAT 2 cut(s) 1989, 4276
Bsu36I CCTNAGG 1 cut(s) 1698
BsuI GTATCC 4 cut(s) 1624, 4299, 4336, 4557
BsuRI GGCC 6 cut(s) 638, 670, 1861, 3127, 3391, 4029
BsuTUI ATCGAT 2 cut(s) 1989, 4276
BtgI CCRYGG 6 cut(s) 17, 633, 871, 1874, 2160, 3525
BtrI CACGTC 1 cut(s) 2684
BtsI GCAGTG 4 cut(s) 1066, 2427, 3786, 3983
BveI ACCTGC 2 cut(s) 820, 2471
CciI TCATGA 2 cut(s) 2178, 2773
CfoI GCGC 6 cut(s) 15, 349, 1124, 1783, 2496, 3952
Cfr10I RCCGGY 1 cut(s) 4417
Cfr42I CCGCGG 1 cut(s) 2163
ClaI ATCGAT 2 cut(s) 1989, 4276
CseI GACGC 1 cut(s) 1097
Csp6I GTAC 4 cut(s) 1158, 1182, 3552, 3768
CspAI ACCGGT 1 cut(s) 4417
CviQI GTAC 4 cut(s) 1158, 1182, 3552, 3768
DinI GGCGCC 3 cut(s) 14, 1782, 3951
DraI TTTAAA 1 cut(s) 3256
EaeI YGGCCR 3 cut(s) 636, 668, 3389
EciI GGCGGA 1 cut(s) 3668
Ecl136II GAGCTC 2 cut(s) 3658, 3749
Eco130I CCWWGG 9 cut(s) 8, 189, 633, 871, 1531, 1874, 2298, 3525, 4178
Eco24I GRGCYC 4 cut(s) 124, 562, 3660, 3751
Eco31I GGTCTC 1 cut(s) 802
Eco32I GATATC 1 cut(s) 2236
Eco47I GGWCC 9 cut(s) 194, 534, 686, 698, 952, 990, 2295, 3514, 3718
Eco53kI GAGCTC 2 cut(s) 3658, 3749
Eco57I CTGAAG 6 cut(s) 714, 943, 1011, 3737, 3766, 4543
Eco81I CCTNAGG 1 cut(s) 1698
EcoICRI GAGCTC 2 cut(s) 3658, 3749
EcoNI CCTNNNNNAGG 1 cut(s) 4182
EcoO109I RGGNCCY 2 cut(s) 194, 3718
EcoRI GAATTC 1 cut(s) 420
EcoRII CCWGG 8 cut(s) 291, 477, 536, 1053, 1294, 1708, 2962, 4041
EcoRV GATATC 1 cut(s) 2236
EcoT14I CCWWGG 9 cut(s) 8, 189, 633, 871, 1531, 1874, 2298, 3525, 4178
EcoT22I ATGCAT 1 cut(s) 1931
EcoT38I GRGCYC 4 cut(s) 124, 562, 3660, 3751
EgeI GGCGCC 3 cut(s) 14, 1782, 3951
EheI GGCGCC 3 cut(s) 14, 1782, 3951
ErhI CCWWGG 9 cut(s) 8, 189, 633, 871, 1531, 1874, 2298, 3525, 4178
Esp3I CGTCTC 1 cut(s) 1293
FalI AAGNNNNNCTT 4 cut(s) 365, 397, 2594, 2626
FaqI GGGAC 8 cut(s) 87, 207, 672, 1095, 2093, 3026, 3527, 3731
FauI CCCGC 4 cut(s) 2167, 3051, 3141, 4476
FauNDI CATATG 2 cut(s) 904, 3331
FbaI TGATCA 1 cut(s) 2002
FriOI GRGCYC 4 cut(s) 124, 562, 3660, 3751
GlaI GCGC 6 cut(s) 14, 348, 1123, 1782, 2495, 3951
GsaI CCCAGC 1 cut(s) 1147
GsuI CTGGAG 6 cut(s) 186, 1053, 1149, 1782, 2801, 4275
HaeII RGCGCY 4 cut(s) 16, 1784, 2497, 3953
HaeIII GGCC 6 cut(s) 638, 670, 1861, 3127, 3391, 4029
HgaI GACGC 1 cut(s) 1097
HhaI GCGC 6 cut(s) 15, 349, 1124, 1783, 2496, 3952
Hin1I GRCGYC 5 cut(s) 13, 799, 1108, 1781, 3950
Hin6I GCGC 6 cut(s) 13, 347, 1122, 1781, 2494, 3950
HinP1I GCGC 6 cut(s) 13, 347, 1122, 1781, 2494, 3950
HincII GTYRAC 1 cut(s) 2541
HindII GTYRAC 1 cut(s) 2541
HindIII AAGCTT 2 cut(s) 234, 4214
HphI GGTGA 8 cut(s) 283, 1337, 1342, 2143, 3514, 3589, 3592, 4163
Hpy166II GTNNAC 4 cut(s) 220, 2245, 2541, 2883
Hpy8I GTNNAC 4 cut(s) 220, 2245, 2541, 2883
HpyAV CCTTC 9 cut(s) 50, 262, 442, 455, 1370, 2381, 3180, 3367, 4365
HpyCH4III ACNGT 6 cut(s) 139, 369, 1960, 2449, 3159, 3572
HpyCH4IV ACGT 3 cut(s) 799, 1184, 2683
HpySE526I ACGT 3 cut(s) 799, 1184, 2683
Hsp92I GRCGYC 5 cut(s) 13, 799, 1108, 1781, 3950
HspAI GCGC 6 cut(s) 13, 347, 1122, 1781, 2494, 3950
KasI GGCGCC 3 cut(s) 12, 1780, 3949
KflI GGGWCCC 2 cut(s) 194, 3718
Kpn2I TCCGGA 2 cut(s) 1684, 2638
KpnI GGTACC 1 cut(s) 3771
Ksp22I TGATCA 1 cut(s) 2002
KspI CCGCGG 1 cut(s) 2163
LguI GCTCTTC 1 cut(s) 3756
LweI GCATC 9 cut(s) 193, 334, 607, 2124, 2284, 2662, 2694, 2977, 4527
MaeII ACGT 3 cut(s) 799, 1184, 2683
MaeIII GTNAC 6 cut(s) 283, 800, 1954, 2103, 2449, 4045
MbiI CCGCTC 1 cut(s) 4091
MfeI CAATTG 3 cut(s) 1826, 2741, 3182
MflI RGATCY 3 cut(s) 2173, 2401, 4132
MhlI GDGCHC 8 cut(s) 124, 562, 667, 1054, 1885, 2623, 3660, 3751
MlsI TGGCCA 2 cut(s) 638, 3391
MluNI TGGCCA 2 cut(s) 638, 3391
Mly113I GGCGCC 3 cut(s) 13, 1781, 3950
MlyI GAGTC 7 cut(s) 370, 653, 1111, 1816, 2875, 3588, 4074
MmeI TCCRAC 1 cut(s) 3977
Mox20I TGGCCA 2 cut(s) 638, 3391
Mph1103I ATGCAT 1 cut(s) 1931
MroI TCCGGA 2 cut(s) 1684, 2638
MroXI GAANNNNTTC 3 cut(s) 1662, 1752, 4419
MscI TGGCCA 2 cut(s) 638, 3391
MslI CAYNNNNRTG 6 cut(s) 1272, 2447, 3356, 3500, 3524, 3944
Msp20I TGGCCA 2 cut(s) 638, 3391
MspA1I CMGCKG 6 cut(s) 743, 1031, 1571, 1802, 2162, 2792
MunI CAATTG 3 cut(s) 1826, 2741, 3182
Mva1269I GAATGC 1 cut(s) 1237
MvaI CCWGG 8 cut(s) 293, 479, 538, 1055, 1296, 1710, 2964, 4043
MvnI CGCG 2 cut(s) 1122, 2162
NarI GGCGCC 3 cut(s) 13, 1781, 3950
NciI CCSGG 4 cut(s) 1227, 1758, 3345, 4303
NcoI CCATGG 4 cut(s) 633, 871, 1874, 3525
NdeI CATATG 2 cut(s) 904, 3331
NmeAIII GCCGAG 1 cut(s) 646
NmuCI GTSAC 4 cut(s) 800, 1954, 2103, 2449
NsiI ATGCAT 1 cut(s) 1931
NspI RCATGY 4 cut(s) 1136, 1310, 1870, 2270
NspV TTCGAA 2 cut(s) 1501, 3200
OliI CACNNNNGTG 1 cut(s) 2447
PaeI GCATGC 2 cut(s) 1136, 2270
PagI TCATGA 2 cut(s) 2178, 2773
PasI CCCWGGG 1 cut(s) 1054
PciSI GCTCTTC 1 cut(s) 3756
PcsI WCGNNNNNNNCGW 2 cut(s) 25, 3721
PctI GAATGC 1 cut(s) 1237
PdmI GAANNNNTTC 3 cut(s) 1662, 1752, 4419
PfeI GAWTC 8 cut(s) 299, 486, 1948, 2067, 2458, 3698, 4426, 4520
PflFI GACNNNGTC 1 cut(s) 2685
PflMI CCANNNNNTGG 1 cut(s) 2413
PfoI TCCNGGA 6 cut(s) 291, 477, 1225, 1756, 2962, 4301
PinAI ACCGGT 1 cut(s) 4417
PleI GAGTC 7 cut(s) 370, 652, 1111, 1816, 2874, 3588, 4074
PluTI GGCGCC 3 cut(s) 16, 1784, 3953
PpsI GAGTC 7 cut(s) 370, 652, 1111, 1816, 2874, 3588, 4074
PpuMI RGGWCCY 2 cut(s) 194, 3718
Psp124BI GAGCTC 2 cut(s) 3660, 3751
Psp5II RGGWCCY 2 cut(s) 194, 3718
Psp6I CCWGG 8 cut(s) 291, 477, 536, 1053, 1294, 1708, 2962, 4041
PspFI CCCAGC 1 cut(s) 1143
PspGI CCWGG 8 cut(s) 291, 477, 536, 1053, 1294, 1708, 2962, 4041
PspPPI RGGWCCY 2 cut(s) 194, 3718
PsuI RGATCY 3 cut(s) 2173, 2401, 4132
PsyI GACNNNGTC 1 cut(s) 2685
PvuII CAGCTG 2 cut(s) 743, 1031
RsaI GTAC 4 cut(s) 1159, 1183, 3553, 3769
RsaNI GTAC 4 cut(s) 1158, 1182, 3552, 3768
RseI CAYNNNNRTG 6 cut(s) 1272, 2447, 3356, 3500, 3524, 3944
SacI GAGCTC 2 cut(s) 3660, 3751
SacII CCGCGG 1 cut(s) 2163
SapI GCTCTTC 1 cut(s) 3756
SchI GAGTC 7 cut(s) 370, 653, 1111, 1816, 2875, 3588, 4074
SduI GDGCHC 8 cut(s) 124, 562, 667, 1054, 1885, 2623, 3660, 3751
SfaNI GCATC 9 cut(s) 193, 334, 607, 2124, 2284, 2662, 2694, 2977, 4527
SfcI CTRYAG 3 cut(s) 1668, 1743, 3066
SfoI GGCGCC 3 cut(s) 14, 1782, 3951
Sfr303I CCGCGG 1 cut(s) 2163
SfuI TTCGAA 2 cut(s) 1501, 3200
SgrBI CCGCGG 1 cut(s) 2163
SinI GGWCC 9 cut(s) 194, 534, 686, 698, 952, 990, 2295, 3514, 3718
SmiMI CAYNNNNRTG 6 cut(s) 1272, 2447, 3356, 3500, 3524, 3944
SmlI CTYRAG 4 cut(s) 2248, 2807, 3482, 4082
SmoI CTYRAG 4 cut(s) 2248, 2807, 3482, 4082
SphI GCATGC 2 cut(s) 1136, 2270
SspDI GGCGCC 3 cut(s) 12, 1780, 3949
SspI AATATT 1 cut(s) 2289
SstI GAGCTC 2 cut(s) 3660, 3751
StyI CCWWGG 9 cut(s) 8, 189, 633, 871, 1531, 1874, 2298, 3525, 4178
TaaI ACNGT 6 cut(s) 139, 369, 1960, 2449, 3159, 3572
TaiI ACGT 3 cut(s) 802, 1187, 2686
TaqII GACCGA 3 cut(s) 1057, 2091, 4449
TauI GCSGC 3 cut(s) 2165, 2752, 3212
TfiI GAWTC 8 cut(s) 299, 486, 1948, 2067, 2458, 3698, 4426, 4520
TseFI GTSAC 4 cut(s) 800, 1954, 2103, 2449
Tsp45I GTSAC 4 cut(s) 800, 1954, 2103, 2449
TspGWI ACGGA 4 cut(s) 1965, 2468, 4112, 4539
Tth111I GACNNNGTC 1 cut(s) 2685
Van91I CCANNNNNTGG 1 cut(s) 2413
VpaK11BI GGWCC 9 cut(s) 194, 534, 686, 698, 952, 990, 2295, 3514, 3718
XagI CCTNNNNNAGG 1 cut(s) 4182
XapI RAATTY 5 cut(s) 420, 2123, 3737, 3908, 4139
XbaI TCTAGA 1 cut(s) 2678
XceI RCATGY 4 cut(s) 1136, 1310, 1870, 2270
XcmI CCANNNNNNNNNTGG 3 cut(s) 646, 1091, 3507
XmaJI CCTAGG 1 cut(s) 8
XmnI GAANNNNTTC 3 cut(s) 1662, 1752, 4419
ZraI GACGTC 1 cut(s) 800
Zsp2I ATGCAT 1 cut(s) 1931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.