FvH4_4g05523

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000094
Physical Location & Seq
Forward (+)
4812080 .. 4815151
3072 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05523.t1

Sequence Viewer

Length: 3072 bp
ATGCCTCCTAGGCGCCCACGGCAACCCCGTGTGGCTACTTCGACCAATGCCGATGAAGGCAATGAGGTCAGGGGACTGGTTGATGATATTGGGAATATGCTGAGGGATGTTATGGATCGAGCTCCACAACCTCATTCTGTGAAAAGGAGAGAAGTTTTTGAAGCTGGAGCCAGAGAGTTCAAGGGTGCCAAGGGACCCCTTGATGCTTATACTTGGGTGGACAAAATGGAAAAAGCTTTTGTGAGCACAGAGTTGCCAGATGAAAAGAAGGTGAAGATGGCAGTAACTTTCCTGGATGATTCAGCACATCACTGGTGGTCGCTTGCCAGCAGGAATGTTGAGGATGCGCTGAATATGTCGTGGGAACAGTTTAAGACTCTCTTCTTGGGGCAATATTTCAGTCCTGCCCATCGCAGCAGAATTCAGTCAGATTTCCTGAATATAAAGAAGAGGGATGATGAAGGCATTATAGAGTTCCAAGAGAGATTCACTTCCCTGAGCTATCATGTGTCGTATTTGGTTCCTGATGAGAGGACCAGGATGGAGATGTTCATTGGGGCACTTGGTGGAGTGTTTGCGGATAAGATGACGAGTGTTTATTACCCGACTTTCTCTGACGCTGTTCATGCAGCCATGGCCATTGAGTCTATGGGGATTTATAGTGCTCGGCCACGAGATTTCGGTGGTCCCAGTCAGGGTCCATCCAAGAAAGCTGTTTCATCATCCGCTTCAGGTTCATCAGCTGGTAGTGGCAGTAGTGGGGGATCAGGTTCTCCTTCTCGGTTTAGGGTTAGAGGACGTCACAGGAGACCTGCTAGGAGCCAGTTGGGTAGGCAGCAGTCCGGGCAGTATAGTTCGGGTGGCAGTTTCCATGGTGGGACATCTGGTAGCCGAACTTTTTCTTATGGGCAGCAGCAGCAGTTTTCTGGGTGCTTTGAGTGTGGTAGTCATGACCACTTCAGGAGAGATTGCCCATTGTTGGCTCAGAGGACCACTCCCACCCCTACTCAGCCTATTGGTCAGTCTTCAGCTGGAGGCTCTAGTAGAGGTGCCCAGGGCAGTGCTTCGGTCAGAGGTGGCTCTCAGCGGGGAAGTGGTCAGCGTGGACGCCCCATGACTCGCGCTAGACTGCATGCTATGACCCAGCAGGAGGGTCGTACTTCTCCAGATGTCATCATCGGTACGTTATTGATTTTTGGTCAGCCTGCTTTTACTTTAATTGATCCCGGAGCCACGCATTCATTCATGTCTAGTAGATTTGCCCTCCATGCCAATGTGCCATCCTCGTCTCTTCTAGGCGAGTGGCATGTGTCTCTACCCTCCGGTGAGGTGATGAGAATAAATTGGGTATTTAGTGGCTGTGAAGTGGTAGTGGAAGGAGTTATTTTCGAGGCTGATTTAATTCCTTTAGATATAGTGGAGTTTGATGTGATCTTGGGAATGGACTTTTTGGAAGCATATAGAGCTATGGTAGATTGTTTTCAAAAAGTAGTGGTGCTTCGAAGTCCAGATGGTTTTGAGGTTGCTTTCCAAGGGGAAAGAGATGTTATTTTTAGTTGCATAATTTCCGCTGTTGCTGCAAGGAAATTGTTGAGTAAAGGCTGTCAGGCATTTCTAGCTCATGTGGTGGATACAAGTATGGGAGTTGTGGGATTAGAAGATATTCCTGTAGTAAGAGAGTTTCCGGATGTTTTTCCTGAGGAGTTGCCTGGTTTGCCTCCTGCAAGGGATATAGACTTCTCTATAGAATTACTTCCCGGAACTATGCCGATCTCACAGGCGCCTTACAGAATGGCTCCAGCGGAATTGAAAGAGTTGAAGACTCAACTGCAGGAGTTGGTAGATAAGGGTTTTATTCGGCCTAGCATGTCTCCATGGGGTGCTCCAGTGTTGTTTGTTAAGAAGAAAGATGGCACCATGAGACTATGCATTGATTATAGGAAGCTGAATCAGGTCACAGTGAAGAATAAATATCCGTTGCCTCGGATCGATGATTTATTTGATCAGTTGAGGGGTGCCTCTGTTTTCTCCAAGATTGATTTGAGATCGGGGTATCATCAGTTGCGAATTCGAGAGGGTGACATAGCCAAGACTGCTTTTCGGTCACGATATGGTCATTATGAGTTTGTGGTGATGCCATTTGGACTTACTAATGCACCCGCGGCGTTCATGGATCTCATGAATAGGGTGTTTAGTCCATATCTTGATCGTTTCGTGATCGTATTCATAGATGACATTTTGGTTTACTCAAGAAGTAAGAAAGAGCATGCCAAGCATCTAAGAATAATATTGCGGACTTTGGAAAAGTCGCAGTTATTTGCTAAGTTTAGCAAGTGTGAGTTTTGGCTTGATAAAGTGGGGTTCTTGGGTCATGTGATTTCGGCAGAAGGAGTTAGTGTGGATCCCCAAAAGGTGGAAGCAGTGTCGAATTGGAGAAGACCTACCACTGTGACGGAGATTCGTAGTTTCTTGGGATTAGCAGGTTATTATCGGCGCTTTGTGAAAGATTTTTCAAAGATTGCTACTCCTCTCACAAAGTTGACCAGAAAAGGGGTTAAGTATGAATGGTCAGATCAGTGCGAGCAAAATTTTCAAGAATTGAAGAATTGCTTGACAAGTGCTCCTGTTTTGGCACTTCCGGATGATAGTGGAGAATATGTGGTCTATTGTGATGCTTCTAGACGTGGTCTAGGTGGAGTTTTGATGCAGCATGATCGAGTTATTGCCTACGCTTCTAGGCAATTGAAGCCGCATGAGGGGAATTATCCTACTCATGATCTGGAGCTTGCAGCAGTAGTTTTTGCTCTCAAGTTGTGGAGACACTATCTTTATGGAGCCAAGTGTCAGATATTTACCGATCACAAGAGTCTTCGGTATGTGTTCACTCAGAGAGAGCTTAATATGAGACAGAGAAGATGGATGGAGTTAATCAGTGATTATGATTGCACTATTGAGTATCATCCTGGAAAAGCTAATGTGGTAGCTGATGCTCTTAGTCGAAATCCCTCCATATCTTTATCTCATATAAAGATGGTTCGTGTTCCGCTTCTTTGTGAGTTGCGAGCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve zf-CCHC

Protein Analysis

1024

Amino Acids

114.6

Weight (kDa)

9.09

Isoelectric Point (pI)

44.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 92 - 187 1.4e-14 Retrotransposon gag protein
RVP_2 PF08284 372 - 502 6.4e-33 Retroviral aspartyl protease
RVT_1 PF00078 633 - 793 4.9e-36 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 856 - 950 5.2e-32 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 882 - 980 7.6e-33 RNase H-like domain found in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000094)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42190
fragaria_vesca FvH4_1g22371 FvH4_1g23261 FvH4_1g27933 FvH4_1g27934 FvH4_1g29231 FvH4_2g02781 FvH4_2g08031 FvH4_2g08581 FvH4_3g06961 FvH4_3g06962 FvH4_3g13531 FvH4_3g14391 FvH4_3g14392 FvH4_3g18622 FvH4_3g18964 FvH4_3g26314 FvH4_3g28781 FvH4_3g30771 FvH4_3g30772 FvH4_3g31752 FvH4_3g32803 FvH4_3g35751 FvH4_3g35752 FvH4_4g02151 FvH4_4g02642 FvH4_4g05523 FvH4_4g05524 FvH4_4g06574 FvH4_4g06722 FvH4_4g08644 FvH4_4g08645 FvH4_4g08646 FvH4_4g08851 FvH4_4g08971 FvH4_4g09081 FvH4_4g09243 FvH4_4g09244 FvH4_4g09283 FvH4_4g10021 FvH4_4g10032 FvH4_4g10033 FvH4_4g10182 FvH4_4g10361 FvH4_4g12502 FvH4_4g12503 FvH4_4g13341 FvH4_4g13942 FvH4_4g13943 FvH4_4g13944 FvH4_4g14432 FvH4_4g14822 FvH4_4g14823 FvH4_4g15291 FvH4_4g15292 FvH4_4g15293 FvH4_4g15471 FvH4_4g15583 FvH4_4g15584 FvH4_4g15585 FvH4_4g15586 FvH4_4g15587 FvH4_4g15591 FvH4_4g15592 FvH4_4g15593 FvH4_4g16179 FvH4_4g18131 FvH4_4g21031 FvH4_4g22021 FvH4_4g22021 FvH4_4g22042 FvH4_5g03402 FvH4_5g07411 FvH4_5g13881 FvH4_5g15792 FvH4_5g24682 FvH4_5g24911 FvH4_5g25322 FvH4_5g25641 FvH4_5g36692 FvH4_5g38072 FvH4_5g38171 FvH4_6g05202 FvH4_6g08531 FvH4_6g16342 FvH4_6g21771 FvH4_6g23303 FvH4_6g23961 FvH4_6g25381 FvH4_6g27021 FvH4_6g27022 FvH4_6g30091 FvH4_6g30092 FvH4_7g02051 FvH4_7g06031 FvH4_7g07031 FvH4_7g07581 FvH4_7g08381 FvH4_7g11551 FvH4_7g19711 FvH4_7g25621 FvH4_7g29722
malus_domestica MD06G1134500.v1.1 MD09G1067600.v1.1 MD13G1145500.v1.1 MD15G1166500.v1.1 MD16G1270000.v1.1
prunus_persica Prupe.7G030900_v2.0.a1
pyrus_communis pycom01g00710 pycom01g03760 pycom03g10770 pycom04g02180 pycom05g08050 pycom06g04140 pycom06g11110 pycom07g07550 pycom07g21570 pycom08g17240 pycom09g13900 pycom09g19570 pycom10g22300 pycom11g03210 pycom11g13750 pycom11g24890 pycom12561g00270 pycom12668g00020 pycom12g11280 pycom12g20720 pycom12g20730 pycom13g21410 pycom13g21660 pycom14g06010 pycom14g06370 pycom14g12800 pycom16g21440 pycom16g22530 pycom17g02030 pycom420g00170 pycom50g00020 pycom5153684g00110 pycom520g00860 pycom581g00060
rosa_chinensis RchiOBHm_Chr0c08g0498091 RchiOBHm_Chr0c08g0498101 RchiOBHm_Chr1g0326991 RchiOBHm_Chr2g0084311 RchiOBHm_Chr2g0090701 RchiOBHm_Chr2g0098591 RchiOBHm_Chr2g0109491 RchiOBHm_Chr2g0109501 RchiOBHm_Chr2g0130801 RchiOBHm_Chr2g0145931 RchiOBHm_Chr2g0168091 RchiOBHm_Chr3g0457081 RchiOBHm_Chr3g0457091 RchiOBHm_Chr3g0467781 RchiOBHm_Chr3g0467791 RchiOBHm_Chr3g0493771 RchiOBHm_Chr3g0494051 RchiOBHm_Chr3g0496881 RchiOBHm_Chr4g0401391 RchiOBHm_Chr4g0414861 RchiOBHm_Chr4g0424081 RchiOBHm_Chr5g0009611 RchiOBHm_Chr5g0049881 RchiOBHm_Chr5g0050351 RchiOBHm_Chr5g0053791 RchiOBHm_Chr5g0078031 RchiOBHm_Chr6g0256481 RchiOBHm_Chr6g0271231 RchiOBHm_Chr6g0286381 RchiOBHm_Chr7g0197101 RchiOBHm_Chr7g0221891 RchiOBHm_Chr7g0237801 RchiOBHm_Chr7g0237821
rosa_multiflora Rmu_sc0000509.1_g000034 Rmu_sc0004751.1_g000014 Rmu_sc0008546.1_g000002 Rmu_sc0009320.1_g000003 Rmu_sc0010661.1_g000006 Rmu_sc0029652.1_g000002
rosa_roxburghii Rroxscaffold_10G00433150 Rroxscaffold_15G00447630 Rroxscaffold_26G00447790 Rroxscaffold_29G00441810 Rroxscaffold_32G00442860 Rroxscaffold_33G00439800 Rroxscaffold_34G00443230 Rroxscaffold_3G00220560 Rroxscaffold_3G00227370 Rroxscaffold_3G00239080 Rroxscaffold_3G00239090 Rroxscaffold_3G00273190 Rroxscaffold_4G00323530 Rroxscaffold_4G00326840 Rroxscaffold_57G00443110 Rroxscaffold_5G00338590 Rroxscaffold_5G00347530 Rroxscaffold_68G00446990 Rroxscaffold_76G00448570 Rroxscaffold_90G00448860
rosa_rugosa Rorug06G0233600
rosa_samantha Rh3CG302700 Rh6DG286700
rosa_wichuraiana Rw0G002280 Rw0G004080 Rw0G011830 Rw1G003690 Rw1G008800 Rw1G009130 Rw1G009140 Rw1G009150 Rw1G009160 Rw1G009410 Rw1G009580 Rw1G012770 Rw1G015850 Rw1G026860 Rw2G003780 Rw2G006480 Rw2G016520 Rw2G016960 Rw2G018170 Rw2G025180 Rw2G025510 Rw2G025610 Rw2G025750 Rw2G025920 Rw2G029950 Rw2G046360 Rw2G046370 Rw2G046380 Rw3G009070 Rw3G025820 Rw3G027340 Rw3G027900 Rw3G028150 Rw3G029060 Rw4G002440 Rw4G005960 Rw4G007520 Rw4G011970 Rw4G011980 Rw4G012190 Rw4G012530 Rw4G018490 Rw4G026380 Rw5G003970 Rw5G030920 Rw5G031780 Rw5G031900 Rw5G033030 Rw5G033040 Rw5G036330 Rw5G039730 Rw5G048110 Rw6G006670 Rw6G007910 Rw6G009720 Rw6G010990 Rw6G012930 Rw6G012940 Rw6G013420 Rw6G014690 Rw6G015290 Rw6G015790 Rw6G015920 Rw6G036510 Rw7G016650 Rw7G023140 Rw7G028650 Rw7G036450 Rw7G036460 Rw7G036910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 802
Acc36I ACCTGC 2 cut(s) 820, 2471
AccB1I GGYRCC 6 cut(s) 12, 185, 1049, 1780, 1913, 2015
AccB7I CCANNNNNTGG 1 cut(s) 2413
AccII CGCG 2 cut(s) 1122, 2162
AccIII TCCGGA 2 cut(s) 1684, 2638
AclWI GGATC 7 cut(s) 123, 772, 1217, 1994, 2181, 2396, 2409
AcoI YGGCCR 2 cut(s) 636, 668
AcsI RAATTY 3 cut(s) 420, 2067, 2587
AcuI CTGAAG 3 cut(s) 714, 943, 1011
AcyI GRCGYC 4 cut(s) 13, 799, 1108, 1781
AdeI CACNNNGTG 1 cut(s) 566
AfaI GTAC 2 cut(s) 1159, 1183
AfiI CCNNNNNNNGG 6 cut(s) 695, 979, 1150, 2413, 2550, 2756
AgsI TTSAA 9 cut(s) 161, 181, 1484, 1810, 1819, 2514, 2594, 2602, 2746
AjiI CACGTC 1 cut(s) 2684
AjnI CCWGG 5 cut(s) 291, 536, 1053, 1708, 2962
Alw21I GWGCWC 5 cut(s) 124, 248, 667, 1885, 2623
Alw26I GTCTC 7 cut(s) 802, 1293, 1317, 1875, 1915, 2812, 2899
AlwI GGATC 7 cut(s) 123, 772, 1217, 1994, 2181, 2396, 2409
Aor13HI TCCGGA 2 cut(s) 1684, 2638
AoxI GGCC 3 cut(s) 636, 668, 1859
ApeKI GCWGC 9 cut(s) 414, 629, 835, 910, 913, 916, 1577, 2707, 2789
ApoI RAATTY 3 cut(s) 420, 2067, 2587
Asp700I GAANNNNTTC 3 cut(s) 898, 1662, 1752
AspA2I CCTAGG 1 cut(s) 8
AspLEI GCGC 5 cut(s) 15, 349, 1124, 1783, 2496
AspS9I GGNCC 5 cut(s) 194, 534, 686, 698, 990
AsuC2I CCSGG 3 cut(s) 844, 1227, 1758
AsuHPI GGTGA 5 cut(s) 283, 1337, 1342, 2090, 2143
AsuII TTCGAA 1 cut(s) 1501
AvaII GGWCC 5 cut(s) 194, 534, 686, 698, 990
AvrII CCTAGG 1 cut(s) 8
AxyI CCTNAGG 1 cut(s) 1698
BaeGI GKGCMC 2 cut(s) 562, 1054
BalI TGGCCA 1 cut(s) 638
BamHI GGATCC 1 cut(s) 2401
BanI GGYRCC 6 cut(s) 12, 185, 1049, 1780, 1913, 2015
BanII GRGCYC 1 cut(s) 124
BarI GAAGNNNNNNTAC 2 cut(s) 1482, 1514
BauI CACGAG 1 cut(s) 672
BbsI GAAGAC 4 cut(s) 1017, 1826, 2443, 2861
Bbv12I GWGCWC 5 cut(s) 124, 248, 667, 1885, 2623
BbvCI CCTCAGC 1 cut(s) 101
BbvI GCAGC 9 cut(s) 426, 641, 847, 922, 925, 928, 1564, 2719, 2801
BceAI ACGGC 1 cut(s) 35
BcgI CGANNNNNNTGC 4 cut(s) 1136, 1170, 2696, 2730
BciT130I CCWGG 5 cut(s) 293, 538, 1055, 1710, 2964
BciVI GTATCC 1 cut(s) 1624
BclI TGATCA 1 cut(s) 2002
BcnI CCSGG 3 cut(s) 844, 1227, 1758
BcoDI GTCTC 7 cut(s) 802, 1293, 1317, 1875, 1915, 2812, 2899
BfmI CTRYAG 3 cut(s) 1668, 1743, 1829
BfoI RGCGCY 3 cut(s) 16, 1784, 2497
BfuAI ACCTGC 2 cut(s) 820, 2471
BfuI GTATCC 1 cut(s) 1624
BglI GCCNNNNNGGC 1 cut(s) 10
BlnI CCTAGG 1 cut(s) 8
Bme1390I CCNGG 8 cut(s) 293, 538, 844, 1055, 1227, 1710, 1758, 2964
Bme18I GGWCC 5 cut(s) 194, 534, 686, 698, 990
BmgBI CACGTC 1 cut(s) 2684
BmgT120I GGNCC 5 cut(s) 194, 534, 686, 698, 990
BmrFI CCNGG 8 cut(s) 293, 538, 844, 1055, 1227, 1710, 1758, 2964
BmrI ACTGGG 1 cut(s) 684
BmsI GCATC 7 cut(s) 193, 334, 2124, 2284, 2662, 2694, 2977
BmuI ACTGGG 1 cut(s) 684
BpiI GAAGAC 4 cut(s) 1017, 1826, 2443, 2861
BplI GAGNNNNNCTC 2 cut(s) 979, 1011
BpmI CTGGAG 6 cut(s) 186, 1053, 1149, 1782, 1869, 2801
Bpu10I CCTNAGC 2 cut(s) 101, 497
Bpu14I TTCGAA 1 cut(s) 1501
BpuEI CTTGAG 2 cut(s) 2233, 2792
BpuMI CCSGG 3 cut(s) 844, 1227, 1758
Bsa29I ATCGAT 1 cut(s) 1989
BsaHI GRCGYC 4 cut(s) 13, 799, 1108, 1781
BsaI GGTCTC 1 cut(s) 802
BsaWI WCCGGW 3 cut(s) 1322, 1684, 2638
BsaXI ACNNNNNCTCC 6 cut(s) 2382, 2412, 2605, 2635, 2643, 2673
Bsc4I CCNNNNNNNGG 6 cut(s) 695, 979, 1150, 2413, 2550, 2756
Bse1I ACTGG 5 cut(s) 81, 317, 690, 823, 1886
Bse21I CCTNAGG 1 cut(s) 1698
Bse3DI GCAATG 1 cut(s) 67
BseAI TCCGGA 2 cut(s) 1684, 2638
BseBI CCWGG 5 cut(s) 293, 538, 1055, 1710, 2964
BseCI ATCGAT 1 cut(s) 1989
BseLI CCNNNNNNNGG 6 cut(s) 695, 979, 1150, 2413, 2550, 2756
BseMI GCAATG 1 cut(s) 67
BseMII CTCAG 7 cut(s) 92, 488, 998, 1022, 1097, 1689, 2900
BseNI ACTGG 5 cut(s) 81, 317, 690, 823, 1886
BseRI GAGGAG 2 cut(s) 1715, 2517
BseSI GKGCMC 2 cut(s) 562, 1054
BseXI GCAGC 9 cut(s) 426, 641, 847, 922, 925, 928, 1564, 2719, 2801
BseYI CCCAGC 1 cut(s) 1143
Bsh1236I CGCG 2 cut(s) 1122, 2162
BshFI GGCC 3 cut(s) 638, 670, 1861
BshNI GGYRCC 6 cut(s) 12, 185, 1049, 1780, 1913, 2015
BshVI ATCGAT 1 cut(s) 1989
BsiHKAI GWGCWC 5 cut(s) 124, 248, 667, 1885, 2623
BsiSI CCGG 6 cut(s) 843, 1227, 1323, 1685, 1758, 2639
BslFI GGGAC 4 cut(s) 87, 207, 672, 892
BslI CCNNNNNNNGG 6 cut(s) 695, 979, 1150, 2413, 2550, 2756
BsmAI GTCTC 7 cut(s) 802, 1293, 1317, 1875, 1915, 2812, 2899
BsmBI CGTCTC 1 cut(s) 1293
BsmFI GGGAC 4 cut(s) 87, 207, 672, 892
BsmI GAATGC 1 cut(s) 1237
BsnI GGCC 3 cut(s) 638, 670, 1861
Bso31I GGTCTC 1 cut(s) 802
Bsp119I TTCGAA 1 cut(s) 1501
Bsp1286I GDGCHC 7 cut(s) 124, 248, 562, 667, 1054, 1885, 2623
Bsp13I TCCGGA 2 cut(s) 1684, 2638
Bsp19I CCATGG 3 cut(s) 633, 871, 1874
BspANI GGCC 3 cut(s) 638, 670, 1861
BspCNI CTCAG 7 cut(s) 93, 489, 997, 1021, 1096, 1690, 2899
BspDI ATCGAT 1 cut(s) 1989
BspEI TCCGGA 2 cut(s) 1684, 2638
BspFNI CGCG 2 cut(s) 1122, 2162
BspHI TCATGA 3 cut(s) 949, 2178, 2773
BspMAI CTGCAG 1 cut(s) 1833
BspMI ACCTGC 2 cut(s) 820, 2471
BspPI GGATC 7 cut(s) 123, 772, 1217, 1994, 2181, 2396, 2409
BspT104I TTCGAA 1 cut(s) 1501
BspT107I GGYRCC 6 cut(s) 12, 185, 1049, 1780, 1913, 2015
BspTNI GGTCTC 1 cut(s) 802
BsrDI GCAATG 1 cut(s) 67
BsrI ACTGG 5 cut(s) 81, 317, 690, 823, 1886
BssNI GRCGYC 4 cut(s) 13, 799, 1108, 1781
BssSI CACGAG 1 cut(s) 672
BssT1I CCWWGG 6 cut(s) 8, 189, 633, 871, 1531, 1874
Bst2BI CACGAG 1 cut(s) 672
Bst2UI CCWGG 5 cut(s) 293, 538, 1055, 1710, 2964
Bst4CI ACNGT 3 cut(s) 369, 1960, 2449
Bst6I CTCTTC 3 cut(s) 386, 443, 1296
BstACI GRCGYC 4 cut(s) 13, 799, 1108, 1781
BstBI TTCGAA 1 cut(s) 1501
BstC8I GCNNGC 8 cut(s) 324, 328, 1134, 1206, 2268, 2582, 2787, 3063
BstDSI CCRYGG 5 cut(s) 17, 633, 871, 1874, 2160
BstFNI CGCG 2 cut(s) 1122, 2162
BstH2I RGCGCY 3 cut(s) 16, 1784, 2497
BstHHI GCGC 5 cut(s) 15, 349, 1124, 1783, 2496
BstMAI GTCTC 7 cut(s) 802, 1293, 1317, 1875, 1915, 2812, 2899
BstNI CCWGG 5 cut(s) 293, 538, 1055, 1710, 2964
BstNSI RCATGY 4 cut(s) 1136, 1310, 1870, 2270
BstSCI CCNGG 8 cut(s) 291, 536, 842, 1053, 1225, 1708, 1756, 2962
BstSFI CTRYAG 3 cut(s) 1668, 1743, 1829
BstSLI GKGCMC 2 cut(s) 562, 1054
BstUI CGCG 2 cut(s) 1122, 2162
BstV1I GCAGC 9 cut(s) 426, 641, 847, 922, 925, 928, 1564, 2719, 2801
BstV2I GAAGAC 4 cut(s) 1017, 1826, 2443, 2861
BstX2I RGATCY 2 cut(s) 2173, 2401
BstYI RGATCY 2 cut(s) 2173, 2401
Bsu15I ATCGAT 1 cut(s) 1989
Bsu36I CCTNAGG 1 cut(s) 1698
BsuI GTATCC 1 cut(s) 1624
BsuRI GGCC 3 cut(s) 638, 670, 1861
BsuTUI ATCGAT 1 cut(s) 1989
BtgI CCRYGG 5 cut(s) 17, 633, 871, 1874, 2160
BtgZI GCGATG 1 cut(s) 395
BtrI CACGTC 1 cut(s) 2684
BtsI GCAGTG 2 cut(s) 1066, 2427
BtsIMutI CAGTG 8 cut(s) 310, 1066, 1893, 1965, 2427, 2445, 2582, 2938
BveI ACCTGC 2 cut(s) 820, 2471
Cac8I GCNNGC 8 cut(s) 324, 328, 1134, 1206, 2268, 2582, 2787, 3063
CciI TCATGA 3 cut(s) 949, 2178, 2773
CfoI GCGC 5 cut(s) 15, 349, 1124, 1783, 2496
Cfr13I GGNCC 5 cut(s) 194, 534, 686, 698, 990
Cfr42I CCGCGG 1 cut(s) 2163
ClaI ATCGAT 1 cut(s) 1989
CseI GACGC 2 cut(s) 626, 1116
Csp6I GTAC 2 cut(s) 1158, 1182
CviQI GTAC 2 cut(s) 1158, 1182
DinI GGCGCC 2 cut(s) 14, 1782
DraIII CACNNNGTG 1 cut(s) 566
EaeI YGGCCR 2 cut(s) 636, 668
Eam1104I CTCTTC 3 cut(s) 386, 443, 1296
EarI CTCTTC 3 cut(s) 386, 443, 1296
Ecl136II GAGCTC 1 cut(s) 122
Eco130I CCWWGG 6 cut(s) 8, 189, 633, 871, 1531, 1874
Eco24I GRGCYC 1 cut(s) 124
Eco31I GGTCTC 1 cut(s) 802
Eco47I GGWCC 5 cut(s) 194, 534, 686, 698, 990
Eco53kI GAGCTC 1 cut(s) 122
Eco57I CTGAAG 3 cut(s) 714, 943, 1011
Eco81I CCTNAGG 1 cut(s) 1698
EcoICRI GAGCTC 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 194
EcoRI GAATTC 2 cut(s) 420, 2067
EcoRII CCWGG 5 cut(s) 291, 536, 1053, 1708, 2962
EcoT14I CCWWGG 6 cut(s) 8, 189, 633, 871, 1531, 1874
EcoT22I ATGCAT 1 cut(s) 1931
EcoT38I GRGCYC 1 cut(s) 124
EgeI GGCGCC 2 cut(s) 14, 1782
EheI GGCGCC 2 cut(s) 14, 1782
ErhI CCWWGG 6 cut(s) 8, 189, 633, 871, 1531, 1874
Esp3I CGTCTC 1 cut(s) 1293
FalI AAGNNNNNCTT 4 cut(s) 365, 397, 2594, 2626
FaqI GGGAC 4 cut(s) 87, 207, 672, 892
FauI CCCGC 2 cut(s) 1080, 2167
FbaI TGATCA 1 cut(s) 2002
FriOI GRGCYC 1 cut(s) 124
GlaI GCGC 5 cut(s) 14, 348, 1123, 1782, 2495
GsaI CCCAGC 1 cut(s) 1147
GsuI CTGGAG 6 cut(s) 186, 1053, 1149, 1782, 1869, 2801
HaeII RGCGCY 3 cut(s) 16, 1784, 2497
HaeIII GGCC 3 cut(s) 638, 670, 1861
HapII CCGG 6 cut(s) 843, 1227, 1323, 1685, 1758, 2639
HgaI GACGC 2 cut(s) 626, 1116
HhaI GCGC 5 cut(s) 15, 349, 1124, 1783, 2496
Hin1I GRCGYC 4 cut(s) 13, 799, 1108, 1781
Hin6I GCGC 5 cut(s) 13, 347, 1122, 1781, 2494
HinP1I GCGC 5 cut(s) 13, 347, 1122, 1781, 2494
HincII GTYRAC 1 cut(s) 2541
HindII GTYRAC 1 cut(s) 2541
HindIII AAGCTT 1 cut(s) 234
HinfI GANTC 9 cut(s) 299, 376, 486, 644, 1117, 1822, 1948, 2458, 2866
HpaII CCGG 6 cut(s) 843, 1227, 1323, 1685, 1758, 2639
HphI GGTGA 5 cut(s) 283, 1337, 1342, 2090, 2143
Hpy166II GTNNAC 5 cut(s) 220, 1106, 2245, 2541, 2883
Hpy188I TCNGA 8 cut(s) 430, 616, 987, 1073, 1986, 2572, 2847, 2889
Hpy8I GTNNAC 5 cut(s) 220, 1106, 2245, 2541, 2883
HpyAV CCTTC 6 cut(s) 50, 262, 455, 786, 1370, 2381
HpyCH4III ACNGT 3 cut(s) 369, 1960, 2449
HpyCH4IV ACGT 3 cut(s) 799, 1184, 2683
HpySE526I ACGT 3 cut(s) 799, 1184, 2683
Hsp92I GRCGYC 4 cut(s) 13, 799, 1108, 1781
HspAI GCGC 5 cut(s) 13, 347, 1122, 1781, 2494
KasI GGCGCC 2 cut(s) 12, 1780
KflI GGGWCCC 1 cut(s) 194
Kpn2I TCCGGA 2 cut(s) 1684, 2638
Ksp22I TGATCA 1 cut(s) 2002
KspI CCGCGG 1 cut(s) 2163
LmnI GCTCC 9 cut(s) 127, 167, 819, 1229, 1801, 1888, 2626, 2782, 2834
Lsp1109I GCAGC 9 cut(s) 426, 641, 847, 922, 925, 928, 1564, 2719, 2801
LweI GCATC 7 cut(s) 193, 334, 2124, 2284, 2662, 2694, 2977
MaeII ACGT 3 cut(s) 799, 1184, 2683
MaeIII GTNAC 6 cut(s) 283, 800, 1954, 2078, 2103, 2449
MfeI CAATTG 1 cut(s) 2741
MflI RGATCY 2 cut(s) 2173, 2401
MhlI GDGCHC 7 cut(s) 124, 248, 562, 667, 1054, 1885, 2623
MlsI TGGCCA 1 cut(s) 638
MluNI TGGCCA 1 cut(s) 638
Mly113I GGCGCC 2 cut(s) 13, 1781
MlyI GAGTC 5 cut(s) 370, 653, 1111, 1816, 2875
Mox20I TGGCCA 1 cut(s) 638
Mph1103I ATGCAT 1 cut(s) 1931
MroI TCCGGA 2 cut(s) 1684, 2638
MroXI GAANNNNTTC 3 cut(s) 898, 1662, 1752
MscI TGGCCA 1 cut(s) 638
MseI TTAA 7 cut(s) 372, 1217, 1400, 1899, 2556, 2898, 2927
MslI CAYNNNNRTG 1 cut(s) 1272
Msp20I TGGCCA 1 cut(s) 638
MspA1I CMGCKG 6 cut(s) 743, 1031, 1087, 1571, 1802, 2162
MspI CCGG 6 cut(s) 843, 1227, 1323, 1685, 1758, 2639
MspR9I CCNGG 8 cut(s) 293, 538, 844, 1055, 1227, 1710, 1758, 2964
MunI CAATTG 1 cut(s) 2741
Mva1269I GAATGC 1 cut(s) 1237
MvaI CCWGG 5 cut(s) 293, 538, 1055, 1710, 2964
MvnI CGCG 2 cut(s) 1122, 2162
NarI GGCGCC 2 cut(s) 13, 1781
NciI CCSGG 3 cut(s) 844, 1227, 1758
NcoI CCATGG 3 cut(s) 633, 871, 1874
NmeAIII GCCGAG 1 cut(s) 646
NmuCI GTSAC 5 cut(s) 800, 1954, 2078, 2103, 2449
NsiI ATGCAT 1 cut(s) 1931
NspI RCATGY 4 cut(s) 1136, 1310, 1870, 2270
NspV TTCGAA 1 cut(s) 1501
PaeI GCATGC 2 cut(s) 1136, 2270
PagI TCATGA 3 cut(s) 949, 2178, 2773
PasI CCCWGGG 1 cut(s) 1054
PcsI WCGNNNNNNNCGW 1 cut(s) 25
PctI GAATGC 1 cut(s) 1237
PdmI GAANNNNTTC 3 cut(s) 898, 1662, 1752
PfeI GAWTC 4 cut(s) 299, 486, 1948, 2458
PflFI GACNNNGTC 1 cut(s) 2685
PflMI CCANNNNNTGG 1 cut(s) 2413
PfoI TCCNGGA 4 cut(s) 291, 1225, 1756, 2962
PleI GAGTC 5 cut(s) 370, 652, 1111, 1816, 2874
PluTI GGCGCC 2 cut(s) 16, 1784
PpsI GAGTC 5 cut(s) 370, 652, 1111, 1816, 2874
PpuMI RGGWCCY 1 cut(s) 194
Psp124BI GAGCTC 1 cut(s) 124
Psp5II RGGWCCY 1 cut(s) 194
Psp6I CCWGG 5 cut(s) 291, 536, 1053, 1708, 2962
PspFI CCCAGC 1 cut(s) 1143
PspGI CCWGG 5 cut(s) 291, 536, 1053, 1708, 2962
PspPI GGNCC 5 cut(s) 194, 534, 686, 698, 990
PspPPI RGGWCCY 1 cut(s) 194
PstI CTGCAG 1 cut(s) 1833
PsuI RGATCY 2 cut(s) 2173, 2401
PsyI GACNNNGTC 1 cut(s) 2685
PvuII CAGCTG 2 cut(s) 743, 1031
RsaI GTAC 2 cut(s) 1159, 1183
RsaNI GTAC 2 cut(s) 1158, 1182
RseI CAYNNNNRTG 1 cut(s) 1272
SacI GAGCTC 1 cut(s) 124
SacII CCGCGG 1 cut(s) 2163
SaqAI TTAA 7 cut(s) 372, 1217, 1400, 1899, 2556, 2898, 2927
Sau96I GGNCC 5 cut(s) 194, 534, 686, 698, 990
SchI GAGTC 5 cut(s) 370, 653, 1111, 1816, 2875
ScrFI CCNGG 8 cut(s) 293, 538, 844, 1055, 1227, 1710, 1758, 2964
SduI GDGCHC 7 cut(s) 124, 248, 562, 667, 1054, 1885, 2623
SfaNI GCATC 7 cut(s) 193, 334, 2124, 2284, 2662, 2694, 2977
SfcI CTRYAG 3 cut(s) 1668, 1743, 1829
SfoI GGCGCC 2 cut(s) 14, 1782
Sfr303I CCGCGG 1 cut(s) 2163
SfuI TTCGAA 1 cut(s) 1501
SgrBI CCGCGG 1 cut(s) 2163
SinI GGWCC 5 cut(s) 194, 534, 686, 698, 990
SmiMI CAYNNNNRTG 1 cut(s) 1272
SmlI CTYRAG 2 cut(s) 2248, 2807
SmoI CTYRAG 2 cut(s) 2248, 2807
SphI GCATGC 2 cut(s) 1136, 2270
SspDI GGCGCC 2 cut(s) 12, 1780
SspI AATATT 2 cut(s) 395, 2289
SstI GAGCTC 1 cut(s) 124
StyD4I CCNGG 8 cut(s) 291, 536, 842, 1053, 1225, 1708, 1756, 2962
StyI CCWWGG 6 cut(s) 8, 189, 633, 871, 1531, 1874
TaaI ACNGT 3 cut(s) 369, 1960, 2449
TaiI ACGT 3 cut(s) 802, 1187, 2686
TaqI TCGA 9 cut(s) 41, 118, 1389, 1501, 1989, 2071, 2426, 2716, 2998
TaqII GACCGA 2 cut(s) 1057, 2091
TauI GCSGC 2 cut(s) 2165, 2752
TfiI GAWTC 4 cut(s) 299, 486, 1948, 2458
Tru1I TTAA 7 cut(s) 372, 1217, 1400, 1899, 2556, 2898, 2927
Tru9I TTAA 7 cut(s) 372, 1217, 1400, 1899, 2556, 2898, 2927
TscAI CASTG 8 cut(s) 317, 1066, 1893, 1965, 2427, 2452, 2582, 2938
TseFI GTSAC 5 cut(s) 800, 1954, 2078, 2103, 2449
TseI GCWGC 9 cut(s) 414, 629, 835, 910, 913, 916, 1577, 2707, 2789
Tsp45I GTSAC 5 cut(s) 800, 1954, 2078, 2103, 2449
TspGWI ACGGA 2 cut(s) 1965, 2468
TspRI CASTG 8 cut(s) 317, 1066, 1893, 1965, 2427, 2452, 2582, 2938
Tth111I GACNNNGTC 1 cut(s) 2685
Van91I CCANNNNNTGG 1 cut(s) 2413
VpaK11BI GGWCC 5 cut(s) 194, 534, 686, 698, 990
XapI RAATTY 3 cut(s) 420, 2067, 2587
XbaI TCTAGA 1 cut(s) 2678
XceI RCATGY 4 cut(s) 1136, 1310, 1870, 2270
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XmaJI CCTAGG 1 cut(s) 8
XmnI GAANNNNTTC 3 cut(s) 898, 1662, 1752
ZraI GACGTC 1 cut(s) 800
Zsp2I ATGCAT 1 cut(s) 1931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.