RchiOBHm_Chr2g0145931

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000094
Physical Location & Seq
Forward (+)
63657468 .. 63661983
4516 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51575

Sequence Viewer

Length: 2427 bp
ATGCGTCCTAGACGCCGGACTCGTGGGGAAACCCCTCCCATTCCAGGAGATGAGCATACTCCTGGAGGAATAGCGGAAGCGTTAGGACGTATTGTGCAGCAGTTGACTGCAGCGCTTCCAGGCTCCAGAACTGACTTTACCATGGAGCGAGCGAAGAGGCATGGAGCTTATAGCTTCTCTCATGCTCCTGAGGCTATGGATGCCCAGAATTGGTTGAACAAAATGGAGAGAGTCTTCACTCAAATCCATTGCCCTGAGGATCGAAAAGTGGGCTTAGCGGTGGATTTTCTGGATGGTGTAGCTTTTGATTGGTGGTCTTATATGAGCAGGGACTTAGAGATTGATGGCCCAATTACTTGGGAACAGTTCAAGGAGCACTTTACTGAGAGATATTTTGGCACAGCTATCCGAGATAGGATGAAATATGAGTTCTTACATCTGCAAAAGGGGGACATGACCGTAACAGAATTTGAACAACGGTTCACCCAACTGGCCCAGTTTGTGCCTGATATGGTTAGCACTGAGAGGGAGCGGATTTATAGGTTTGTGGATGCTTTGGGGGGTAAGTATTCTGATCAGCTGACCGGAGTACCATTTACTGATTATGCTGAGGTCGTTAATGCTGCTTTACGACTTGAGACTAGGTATATGTCTGGTATCCGACCTCGGGATTTGGGTGGCCCCAGCCAGGGCCCACCCAAGAAGGCTGCTTCCACTTCTGGGTCAGGATCTTCAGCAGGCAGCGGACAGAGTAGTGGGTCTGGTGCTGGATCTCGTTATAGACGCGGGGGGCGTGACAGGAGATTCCCTCGGGGGCAGTTTAGTGGACGCCCGTTTGGGCAGAGCAGAGTTGGTTTTGGTGGTCAGCATAGGGCTCCAGCTAGGCAGCCAGCCCCGTTTGGGCAGTACCAGTCAGTCGGATGCTTTGAGTGTGGACAGCAGGGCCACTTTAGGAGGGATTGCCCTCTATTGACTCAGAGAGCTGCATTTACTCCTTATCAGACCGCGGGCCAGTCTTCTGCCGGTACGTCTATTAGTGGTACCGTGGCTTCTACTAGCGGTACCCATCATAGTTCAGCAGCCCGTGGCAGCCCGCAGCGGGGTAGGGGTCAGAGAGGACGTCCTACCACTCAGGCTAGGTTGCATGCCATGACGCAGCAGGAGGGCCACGACTCACCAGATGTTATCATTGGTACGTTGTCTGTTTTTGGTCAGCCTGCTTATACTTTAATTGATCCTGGTGCATCGCATTCTTTTATGTCGAGTAGATTTGCATGTTTTGCGAATGTGCCATCATCACTTCTTATTGGTGAATGGAATGTGTTATTACCTGCTGGAGCATCACTTAAGATAGAATGGGTTTTTAGAGGTTGTGATGTTTTGATTGAGGGTGTTAGTTTGGAGGTTGATTTAATTCCATTAGACCTTGTTGAATTTGATGTGATTTTGGGGATGGAGTTTTTGGCGACTCATCGGGCTAATATTGATTGCTTTCGTAAGATTGTGGTCTTTCGAAGTCCAGGAAAGCCAATGATTACCTTTCAGGGAGAACGTAATGTTCTAGCTTCCTGTATGATTTCCGCTTTGACCGCTGAGAAGCTGTTGAATAAGGGAAGTCAGGCTTATTTGGCACATGTGGTGGACACTAATAGGGAAGAGCTGAGTATTAGAGAGATACCAGTGGTTAGGAAATTTCAGGATGTATTTCCTGACGAATTACCTGGTTTACCTCCTGCTAGGGAGATAGATTTCACTATTGATTTACTCCCTGGCACCACACCCATATCTTTGGCACCATACAGAATGGCCCCAGCTGAATTAAGAGAGTTGAAGATTCAGTTGCAAGAACTAGTGGATAAGGGGTTCATCCAATCTAGTGTGTCTCCTTGGGGTGCCCCGGTTTTATTTGTTAAGAAGAAGGATGGTTCTTTACGGTTGTGCATTGATTATCGAAAGTTGAATAGGGTTACTGTGAAGAATAAATACCCTTTGCCTCGTATTGATGACCTCTTTGATCAGTTGAGGGGAGCCAAGGTCTTTTCCAAAATCGATTTGAGGTCTGGTTACCATCAGTTGAGGATAAGGGAGTCTGATGTACCCAAGACTGCATTTCGATCACGTTATGGGCACTACGAATTCAGAGTGATGCCGTTTGGGTTGACTAATGCCCCTGCAGCTTTTATGGACCTCATGAATAGAGTGTTCCGTCCTTACCTTGATCGATTTGTGATTGTGTTCATTGATGATATCTTGGTGTATTCCAAGGACAATAGTCAACATGCCAAGCATCTAAGGATTGTATTGCGAACTTTGAGGGAGGCCCAGTTATTTGCAAAGTTCAATAAATGTGAATTTTGGCTGAATAGTATTGGATTTTGGGTCATGTTGTATCCGCAGAAGGTATCAGTGTGGACCCTCAGAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve zf-CCHC

Protein Analysis

808

Amino Acids

90.82

Weight (kDa)

9.48

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 67 - 187 2.3e-06 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 92 - 186 5.7e-16 Retrotransposon gag protein
RVP_2 PF08284 377 - 505 7.9e-30 Retroviral aspartyl protease
RVT_1 PF00078 637 - 787 4.7e-36 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000094)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42190
fragaria_vesca FvH4_1g22371 FvH4_1g23261 FvH4_1g27933 FvH4_1g27934 FvH4_1g29231 FvH4_2g02781 FvH4_2g08031 FvH4_2g08581 FvH4_3g06961 FvH4_3g06962 FvH4_3g13531 FvH4_3g14391 FvH4_3g14392 FvH4_3g18622 FvH4_3g18964 FvH4_3g26314 FvH4_3g28781 FvH4_3g30771 FvH4_3g30772 FvH4_3g31752 FvH4_3g32803 FvH4_3g35751 FvH4_3g35752 FvH4_4g02151 FvH4_4g02642 FvH4_4g05523 FvH4_4g05524 FvH4_4g06574 FvH4_4g06722 FvH4_4g08644 FvH4_4g08645 FvH4_4g08646 FvH4_4g08851 FvH4_4g08971 FvH4_4g09081 FvH4_4g09243 FvH4_4g09244 FvH4_4g09283 FvH4_4g10021 FvH4_4g10032 FvH4_4g10033 FvH4_4g10182 FvH4_4g10361 FvH4_4g12502 FvH4_4g12503 FvH4_4g13341 FvH4_4g13942 FvH4_4g13943 FvH4_4g13944 FvH4_4g14432 FvH4_4g14822 FvH4_4g14823 FvH4_4g15291 FvH4_4g15292 FvH4_4g15293 FvH4_4g15471 FvH4_4g15583 FvH4_4g15584 FvH4_4g15585 FvH4_4g15586 FvH4_4g15587 FvH4_4g15591 FvH4_4g15592 FvH4_4g15593 FvH4_4g16179 FvH4_4g18131 FvH4_4g21031 FvH4_4g22021 FvH4_4g22021 FvH4_4g22042 FvH4_5g03402 FvH4_5g07411 FvH4_5g13881 FvH4_5g15792 FvH4_5g24682 FvH4_5g24911 FvH4_5g25322 FvH4_5g25641 FvH4_5g36692 FvH4_5g38072 FvH4_5g38171 FvH4_6g05202 FvH4_6g08531 FvH4_6g16342 FvH4_6g21771 FvH4_6g23303 FvH4_6g23961 FvH4_6g25381 FvH4_6g27021 FvH4_6g27022 FvH4_6g30091 FvH4_6g30092 FvH4_7g02051 FvH4_7g06031 FvH4_7g07031 FvH4_7g07581 FvH4_7g08381 FvH4_7g11551 FvH4_7g19711 FvH4_7g25621 FvH4_7g29722
malus_domestica MD06G1134500.v1.1 MD09G1067600.v1.1 MD13G1145500.v1.1 MD15G1166500.v1.1 MD16G1270000.v1.1
prunus_persica Prupe.7G030900_v2.0.a1
pyrus_communis pycom01g00710 pycom01g03760 pycom03g10770 pycom04g02180 pycom05g08050 pycom06g04140 pycom06g11110 pycom07g07550 pycom07g21570 pycom08g17240 pycom09g13900 pycom09g19570 pycom10g22300 pycom11g03210 pycom11g13750 pycom11g24890 pycom12561g00270 pycom12668g00020 pycom12g11280 pycom12g20720 pycom12g20730 pycom13g21410 pycom13g21660 pycom14g06010 pycom14g06370 pycom14g12800 pycom16g21440 pycom16g22530 pycom17g02030 pycom420g00170 pycom50g00020 pycom5153684g00110 pycom520g00860 pycom581g00060
rosa_chinensis RchiOBHm_Chr0c08g0498091 RchiOBHm_Chr0c08g0498101 RchiOBHm_Chr1g0326991 RchiOBHm_Chr2g0084311 RchiOBHm_Chr2g0090701 RchiOBHm_Chr2g0098591 RchiOBHm_Chr2g0109491 RchiOBHm_Chr2g0109501 RchiOBHm_Chr2g0130801 RchiOBHm_Chr2g0145931 RchiOBHm_Chr2g0168091 RchiOBHm_Chr3g0457081 RchiOBHm_Chr3g0457091 RchiOBHm_Chr3g0467781 RchiOBHm_Chr3g0467791 RchiOBHm_Chr3g0493771 RchiOBHm_Chr3g0494051 RchiOBHm_Chr3g0496881 RchiOBHm_Chr4g0401391 RchiOBHm_Chr4g0414861 RchiOBHm_Chr4g0424081 RchiOBHm_Chr5g0009611 RchiOBHm_Chr5g0049881 RchiOBHm_Chr5g0050351 RchiOBHm_Chr5g0053791 RchiOBHm_Chr5g0078031 RchiOBHm_Chr6g0256481 RchiOBHm_Chr6g0271231 RchiOBHm_Chr6g0286381 RchiOBHm_Chr7g0197101 RchiOBHm_Chr7g0221891 RchiOBHm_Chr7g0237801 RchiOBHm_Chr7g0237821
rosa_multiflora Rmu_sc0000509.1_g000034 Rmu_sc0004751.1_g000014 Rmu_sc0008546.1_g000002 Rmu_sc0009320.1_g000003 Rmu_sc0010661.1_g000006 Rmu_sc0029652.1_g000002
rosa_roxburghii Rroxscaffold_10G00433150 Rroxscaffold_15G00447630 Rroxscaffold_26G00447790 Rroxscaffold_29G00441810 Rroxscaffold_32G00442860 Rroxscaffold_33G00439800 Rroxscaffold_34G00443230 Rroxscaffold_3G00220560 Rroxscaffold_3G00227370 Rroxscaffold_3G00239080 Rroxscaffold_3G00239090 Rroxscaffold_3G00273190 Rroxscaffold_4G00323530 Rroxscaffold_4G00326840 Rroxscaffold_57G00443110 Rroxscaffold_5G00338590 Rroxscaffold_5G00347530 Rroxscaffold_68G00446990 Rroxscaffold_76G00448570 Rroxscaffold_90G00448860
rosa_rugosa Rorug06G0233600
rosa_samantha Rh3CG302700 Rh6DG286700
rosa_wichuraiana Rw0G002280 Rw0G004080 Rw0G011830 Rw1G003690 Rw1G008800 Rw1G009130 Rw1G009140 Rw1G009150 Rw1G009160 Rw1G009410 Rw1G009580 Rw1G012770 Rw1G015850 Rw1G026860 Rw2G003780 Rw2G006480 Rw2G016520 Rw2G016960 Rw2G018170 Rw2G025180 Rw2G025510 Rw2G025610 Rw2G025750 Rw2G025920 Rw2G029950 Rw2G046360 Rw2G046370 Rw2G046380 Rw3G009070 Rw3G025820 Rw3G027340 Rw3G027900 Rw3G028150 Rw3G029060 Rw4G002440 Rw4G005960 Rw4G007520 Rw4G011970 Rw4G011980 Rw4G012190 Rw4G012530 Rw4G018490 Rw4G026380 Rw5G003970 Rw5G030920 Rw5G031780 Rw5G031900 Rw5G033030 Rw5G033040 Rw5G036330 Rw5G039730 Rw5G048110 Rw6G006670 Rw6G007910 Rw6G009720 Rw6G010990 Rw6G012930 Rw6G012940 Rw6G013420 Rw6G014690 Rw6G015290 Rw6G015790 Rw6G015920 Rw6G036510 Rw7G016650 Rw7G023140 Rw7G028650 Rw7G036450 Rw7G036460 Rw7G036910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1123
Acc36I ACCTGC 1 cut(s) 1339
Acc65I GGTACC 2 cut(s) 1040, 1061
AccB1I GGYRCC 5 cut(s) 1040, 1061, 1772, 1792, 1892
AccBSI CCGCTC 1 cut(s) 532
AccII CGCG 2 cut(s) 786, 1007
AclWI GGATC 4 cut(s) 267, 736, 778, 1229
AcsI RAATTY 5 cut(s) 467, 1433, 1691, 2135, 2351
AcuI CTGAAG 1 cut(s) 717
AcyI GRCGYC 3 cut(s) 13, 829, 1120
AfaI GTAC 7 cut(s) 591, 908, 1027, 1042, 1063, 1195, 2097
AfeI AGCGCT 1 cut(s) 114
AflII CTTAAG 1 cut(s) 1346
AflIII ACRYGT 1 cut(s) 1633
AgsI TTSAA 8 cut(s) 217, 370, 473, 1433, 1606, 1831, 1960, 2341
AhlI ACTAGT 1 cut(s) 1849
AjnI CCWGG 8 cut(s) 43, 61, 118, 687, 1237, 1519, 1720, 1768
AloI GAACNNNNNNTCC 2 cut(s) 1847, 1879
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 2 cut(s) 632, 1887
AlwI GGATC 4 cut(s) 267, 736, 778, 1229
Ama87I CYCGRG 2 cut(s) 666, 810
Aor51HI AGCGCT 1 cut(s) 114
AoxI GGCC 9 cut(s) 346, 492, 679, 691, 943, 1009, 1165, 1806, 2319
ApaI GGGCCC 1 cut(s) 695
ApoI RAATTY 5 cut(s) 467, 1433, 1691, 2135, 2351
Asp718I GGTACC 2 cut(s) 1040, 1061
AspLEI GCGC 1 cut(s) 115
AsuC2I CCSGG 1 cut(s) 1898
AsuHPI GGTGA 3 cut(s) 475, 1167, 1322
AsuII TTCGAA 1 cut(s) 1513
AvaI CYCGRG 2 cut(s) 666, 810
AvaII GGWCC 2 cut(s) 2185, 2412
AxyI CCTNAGG 2 cut(s) 189, 255
BaeGI GKGCMC 3 cut(s) 695, 1897, 2130
BaeI ACNNNNGTAYC 2 cut(s) 573, 606
BanI GGYRCC 5 cut(s) 1040, 1061, 1772, 1792, 1892
BanII GRGCYC 2 cut(s) 695, 877
BarI GAAGNNNNNNTAC 4 cut(s) 1033, 1065, 1967, 1999
BauI CACGAG 1 cut(s) 21
BbsI GAAGAC 2 cut(s) 226, 1008
Bbv12I GWGCWC 1 cut(s) 378
BbvCI CCTCAGC 1 cut(s) 609
BccI CCATC 7 cut(s) 287, 338, 1074, 1300, 1447, 1916, 2076
BceAI ACGGC 1 cut(s) 2134
BciT130I CCWGG 8 cut(s) 45, 63, 120, 689, 1239, 1521, 1722, 1770
BciVI GTATCC 2 cut(s) 668, 2400
BclI TGATCA 2 cut(s) 574, 2014
BcnI CCSGG 1 cut(s) 1898
BcoDI GTCTC 2 cut(s) 632, 1887
BcuI ACTAGT 1 cut(s) 1849
BfaI CTAG 9 cut(s) 9, 642, 882, 1056, 1137, 1562, 1737, 1850, 1875
BfmI CTRYAG 2 cut(s) 108, 2172
BfoI RGCGCY 1 cut(s) 116
BfrI CTTAAG 1 cut(s) 1346
BfuAI ACCTGC 1 cut(s) 1339
BfuI GTATCC 2 cut(s) 668, 2400
BlpI GCTNAGC 1 cut(s) 274
Bme1390I CCNGG 9 cut(s) 45, 63, 120, 689, 1239, 1521, 1722, 1770, 1898
Bme18I GGWCC 2 cut(s) 2185, 2412
BmeT110I CYCGRG 2 cut(s) 666, 810
BmrFI CCNGG 9 cut(s) 45, 63, 120, 689, 1239, 1521, 1722, 1770, 1898
BmrI ACTGGG 2 cut(s) 490, 2317
BmsI GCATC 7 cut(s) 190, 541, 911, 1253, 1349, 2136, 2296
BmuI ACTGGG 2 cut(s) 490, 2317
BoxI GACNNNNGTC 1 cut(s) 2271
BpiI GAAGAC 2 cut(s) 226, 1008
BplI GAGNNNNNCTC 2 cut(s) 793, 825
BpmI CTGGAG 4 cut(s) 84, 109, 861, 1356
Bpu10I CCTNAGC 1 cut(s) 609
Bpu1102I GCTNAGC 1 cut(s) 274
Bpu14I TTCGAA 1 cut(s) 1513
BpuEI CTTGAG 1 cut(s) 656
BpuMI CCSGG 1 cut(s) 1898
Bsa29I ATCGAT 2 cut(s) 2049, 2221
BsaHI GRCGYC 3 cut(s) 13, 829, 1120
BsaWI WCCGGW 1 cut(s) 584
BsaXI ACNNNNNCTCC 4 cut(s) 2019, 2049, 2309, 2339
Bse118I RCCGGY 1 cut(s) 1022
Bse1I ACTGG 6 cut(s) 495, 496, 910, 1012, 1679, 2323
Bse21I CCTNAGG 2 cut(s) 189, 255
Bse3DI GCAATG 1 cut(s) 247
BseBI CCWGG 8 cut(s) 45, 63, 120, 689, 1239, 1521, 1722, 1770
BseCI ATCGAT 2 cut(s) 2049, 2221
BseGI GGATG 9 cut(s) 205, 298, 423, 556, 926, 1458, 1705, 1866, 1927
BseMI GCAATG 1 cut(s) 247
BseMII CTCAG 9 cut(s) 180, 246, 375, 513, 600, 989, 1145, 1584, 1652
BseNI ACTGG 6 cut(s) 495, 496, 910, 1012, 1679, 2323
BseSI GKGCMC 3 cut(s) 695, 1897, 2130
BseYI CCCAGC 2 cut(s) 683, 1810
BsgI GTGCAG 1 cut(s) 116
Bsh1236I CGCG 2 cut(s) 786, 1007
BshFI GGCC 9 cut(s) 348, 494, 681, 693, 945, 1011, 1167, 1808, 2321
BshNI GGYRCC 5 cut(s) 1040, 1061, 1772, 1792, 1892
BshVI ATCGAT 2 cut(s) 2049, 2221
BsiHKAI GWGCWC 1 cut(s) 378
BsiHKCI CYCGRG 2 cut(s) 666, 810
BsiSI CCGG 4 cut(s) 16, 585, 1023, 1898
BslFI GGGAC 2 cut(s) 344, 464
BsmAI GTCTC 2 cut(s) 632, 1887
BsmFI GGGAC 2 cut(s) 344, 464
BsmI GAATGC 1 cut(s) 1249
BsnI GGCC 9 cut(s) 348, 494, 681, 693, 945, 1011, 1167, 1808, 2321
BsoBI CYCGRG 2 cut(s) 666, 810
Bsp119I TTCGAA 1 cut(s) 1513
Bsp120I GGGCCC 1 cut(s) 691
Bsp1286I GDGCHC 5 cut(s) 378, 695, 877, 1897, 2130
Bsp143I GATC 8 cut(s) 259, 574, 728, 770, 1234, 2014, 2114, 2218
Bsp1720I GCTNAGC 1 cut(s) 274
Bsp19I CCATGG 1 cut(s) 141
BspANI GGCC 9 cut(s) 348, 494, 681, 693, 945, 1011, 1167, 1808, 2321
BspCNI CTCAG 9 cut(s) 181, 247, 376, 514, 601, 988, 1144, 1585, 1653
BspDI ATCGAT 2 cut(s) 2049, 2221
BspFNI CGCG 2 cut(s) 786, 1007
BspHI TCATGA 1 cut(s) 2190
BspMAI CTGCAG 2 cut(s) 112, 2176
BspMI ACCTGC 1 cut(s) 1339
BspPI GGATC 4 cut(s) 267, 736, 778, 1229
BspQI GCTCTTC 1 cut(s) 1650
BspT104I TTCGAA 1 cut(s) 1513
BspT107I GGYRCC 5 cut(s) 1040, 1061, 1772, 1792, 1892
BspTI CTTAAG 1 cut(s) 1346
BsrBI CCGCTC 1 cut(s) 532
BsrDI GCAATG 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 1022
BsrI ACTGG 6 cut(s) 495, 496, 910, 1012, 1679, 2323
BssAI RCCGGY 1 cut(s) 1022
BssMI GATC 8 cut(s) 259, 574, 728, 770, 1234, 2014, 2114, 2218
BssNI GRCGYC 3 cut(s) 13, 829, 1120
BssSI CACGAG 1 cut(s) 21
BssT1I CCWWGG 4 cut(s) 141, 1886, 2031, 2262
Bst2BI CACGAG 1 cut(s) 21
Bst2UI CCWGG 8 cut(s) 45, 63, 120, 689, 1239, 1521, 1722, 1770
Bst4CI ACNGT 6 cut(s) 366, 460, 480, 1045, 1935, 1972
Bst6I CTCTTC 2 cut(s) 149, 1650
BstACI GRCGYC 3 cut(s) 13, 829, 1120
BstAFI CTTAAG 1 cut(s) 1346
BstAPI GCANNNNNTGC 1 cut(s) 1280
BstBI TTCGAA 1 cut(s) 1513
BstC8I GCNNGC 7 cut(s) 150, 739, 891, 1009, 1094, 1146, 1218
BstDSI CCRYGG 4 cut(s) 141, 1005, 1044, 1084
BstEII GGTNACC 1 cut(s) 2063
BstF5I GGATG 9 cut(s) 205, 298, 423, 556, 926, 1458, 1705, 1866, 1927
BstFNI CGCG 2 cut(s) 786, 1007
BstH2I RGCGCY 1 cut(s) 116
BstHHI GCGC 1 cut(s) 115
BstKTI GATC 8 cut(s) 262, 577, 731, 773, 1237, 2017, 2117, 2221
BstMAI GTCTC 2 cut(s) 632, 1887
BstMBI GATC 8 cut(s) 259, 574, 728, 770, 1234, 2014, 2114, 2218
BstMWI GCNNNNNNNGC 6 cut(s) 191, 200, 1280, 1589, 1628, 2174
BstNI CCWGG 8 cut(s) 45, 63, 120, 689, 1239, 1521, 1722, 1770
BstNSI RCATGY 4 cut(s) 1148, 1278, 1637, 2282
BstPAI GACNNNNGTC 1 cut(s) 2271
BstPI GGTNACC 1 cut(s) 2063
BstSCI CCNGG 9 cut(s) 43, 61, 118, 687, 1237, 1519, 1720, 1768, 1896
BstSFI CTRYAG 2 cut(s) 108, 2172
BstSLI GKGCMC 3 cut(s) 695, 1897, 2130
BstUI CGCG 2 cut(s) 786, 1007
BstV2I GAAGAC 2 cut(s) 226, 1008
BstX2I RGATCY 2 cut(s) 728, 770
BstXI CCANNNNNNTGG 2 cut(s) 357, 1789
BstYI RGATCY 2 cut(s) 728, 770
Bsu15I ATCGAT 2 cut(s) 2049, 2221
Bsu36I CCTNAGG 2 cut(s) 189, 255
BsuI GTATCC 2 cut(s) 668, 2400
BsuRI GGCC 9 cut(s) 348, 494, 681, 693, 945, 1011, 1167, 1808, 2321
BsuTUI ATCGAT 2 cut(s) 2049, 2221
BtgI CCRYGG 4 cut(s) 141, 1005, 1044, 1084
BtgZI GCGATG 1 cut(s) 1230
BtsCI GGATG 9 cut(s) 205, 298, 423, 556, 926, 1458, 1705, 1866, 1927
BtsIMutI CAGTG 3 cut(s) 519, 1686, 2412
BveI ACCTGC 1 cut(s) 1339
Cac8I GCNNGC 7 cut(s) 150, 739, 891, 1009, 1094, 1146, 1218
CciI TCATGA 1 cut(s) 2190
CfoI GCGC 1 cut(s) 115
Cfr10I RCCGGY 1 cut(s) 1022
Cfr42I CCGCGG 1 cut(s) 1008
ClaI ATCGAT 2 cut(s) 2049, 2221
CseI GACGC 4 cut(s) 21, 792, 837, 1162
CsiI ACCWGGT 1 cut(s) 1720
Csp6I GTAC 7 cut(s) 590, 907, 1026, 1041, 1062, 1194, 2096
CviQI GTAC 7 cut(s) 590, 907, 1026, 1041, 1062, 1194, 2096
DpnI GATC 8 cut(s) 261, 576, 730, 772, 1236, 2016, 2116, 2220
DpnII GATC 8 cut(s) 259, 574, 728, 770, 1234, 2014, 2114, 2218
Eam1104I CTCTTC 2 cut(s) 149, 1650
EarI CTCTTC 2 cut(s) 149, 1650
Eco130I CCWWGG 4 cut(s) 141, 1886, 2031, 2262
Eco24I GRGCYC 2 cut(s) 695, 877
Eco32I GATATC 1 cut(s) 2248
Eco47I GGWCC 2 cut(s) 2185, 2412
Eco47III AGCGCT 1 cut(s) 114
Eco57I CTGAAG 1 cut(s) 717
Eco81I CCTNAGG 2 cut(s) 189, 255
Eco88I CYCGRG 2 cut(s) 666, 810
Eco91I GGTNACC 1 cut(s) 2063
EcoO109I RGGNCCY 1 cut(s) 691
EcoO65I GGTNACC 1 cut(s) 2063
EcoRI GAATTC 1 cut(s) 2135
EcoRII CCWGG 8 cut(s) 43, 61, 118, 687, 1237, 1519, 1720, 1768
EcoRV GATATC 1 cut(s) 2248
EcoT14I CCWWGG 4 cut(s) 141, 1886, 2031, 2262
EcoT38I GRGCYC 2 cut(s) 695, 877
ErhI CCWWGG 4 cut(s) 141, 1886, 2031, 2262
FalI AAGNNNNNCTT 4 cut(s) 362, 394, 1606, 1638
FaqI GGGAC 2 cut(s) 344, 464
FauI CCCGC 4 cut(s) 779, 1000, 1092, 1101
FbaI TGATCA 2 cut(s) 574, 2014
FokI GGATG 9 cut(s) 212, 305, 430, 563, 933, 1465, 1712, 1853, 1934
FriOI GRGCYC 2 cut(s) 695, 877
FspBI CTAG 9 cut(s) 9, 642, 882, 1056, 1137, 1562, 1737, 1850, 1875
GlaI GCGC 1 cut(s) 114
GsaI CCCAGC 2 cut(s) 687, 1814
GsuI CTGGAG 4 cut(s) 84, 109, 861, 1356
HaeII RGCGCY 1 cut(s) 116
HaeIII GGCC 9 cut(s) 348, 494, 681, 693, 945, 1011, 1167, 1808, 2321
HapII CCGG 4 cut(s) 16, 585, 1023, 1898
HgaI GACGC 4 cut(s) 21, 792, 837, 1162
HhaI GCGC 1 cut(s) 115
Hin1I GRCGYC 3 cut(s) 13, 829, 1120
Hin6I GCGC 1 cut(s) 113
HinP1I GCGC 1 cut(s) 113
HincII GTYRAC 3 cut(s) 105, 2160, 2276
HindII GTYRAC 3 cut(s) 105, 2160, 2276
HinfI GANTC 8 cut(s) 19, 231, 804, 973, 1172, 1468, 1834, 2087
HpaII CCGG 4 cut(s) 16, 585, 1023, 1898
HphI GGTGA 3 cut(s) 475, 1167, 1322
Hpy166II GTNNAC 9 cut(s) 105, 483, 827, 935, 1642, 1727, 2160, 2276, 2412
Hpy188III TCNNGA 8 cut(s) 126, 188, 290, 668, 726, 1697, 1709, 2191
Hpy8I GTNNAC 9 cut(s) 105, 483, 827, 935, 1642, 1727, 2160, 2276, 2412
HpyAV CCTTC 4 cut(s) 697, 1912, 2392, 2415
HpyCH4III ACNGT 6 cut(s) 366, 460, 480, 1045, 1935, 1972
HpyCH4IV ACGT 6 cut(s) 88, 1028, 1120, 1196, 1552, 2119
HpyF10VI GCNNNNNNNGC 6 cut(s) 191, 200, 1280, 1589, 1628, 2174
HpySE526I ACGT 6 cut(s) 88, 1028, 1120, 1196, 1552, 2119
Hsp92I GRCGYC 3 cut(s) 13, 829, 1120
HspAI GCGC 1 cut(s) 113
KpnI GGTACC 2 cut(s) 1044, 1065
Ksp22I TGATCA 2 cut(s) 574, 2014
KspI CCGCGG 1 cut(s) 1008
Kzo9I GATC 8 cut(s) 259, 574, 728, 770, 1234, 2014, 2114, 2218
LguI GCTCTTC 1 cut(s) 1650
LmnI GCTCC 9 cut(s) 128, 145, 164, 190, 373, 529, 880, 1337, 2027
LweI GCATC 7 cut(s) 190, 541, 911, 1253, 1349, 2136, 2296
MabI ACCWGGT 1 cut(s) 1720
MaeI CTAG 9 cut(s) 9, 642, 882, 1056, 1137, 1562, 1737, 1850, 1875
MaeII ACGT 6 cut(s) 88, 1028, 1120, 1196, 1552, 2119
MaeIII GTNAC 4 cut(s) 460, 794, 1966, 2063
MalI GATC 8 cut(s) 261, 576, 730, 772, 1236, 2016, 2116, 2220
MbiI CCGCTC 1 cut(s) 532
MboI GATC 8 cut(s) 259, 574, 728, 770, 1234, 2014, 2114, 2218
MboII GAAGA 8 cut(s) 166, 226, 723, 1008, 1667, 1843, 1927, 1987
MflI RGATCY 2 cut(s) 728, 770
MhlI GDGCHC 5 cut(s) 378, 695, 877, 1897, 2130
MlyI GAGTC 6 cut(s) 13, 240, 967, 1166, 1462, 2096
MmeI TCCRAC 2 cut(s) 685, 898
MseI TTAA 6 cut(s) 618, 1229, 1347, 1412, 1820, 1911
MspA1I CMGCKG 6 cut(s) 580, 744, 1007, 1099, 1592, 1814
MspCI CTTAAG 1 cut(s) 1346
MspI CCGG 4 cut(s) 16, 585, 1023, 1898
MspR9I CCNGG 9 cut(s) 45, 63, 120, 689, 1239, 1521, 1722, 1770, 1898
Mva1269I GAATGC 1 cut(s) 1249
MvaI CCWGG 8 cut(s) 45, 63, 120, 689, 1239, 1521, 1722, 1770
MvnI CGCG 2 cut(s) 786, 1007
MwoI GCNNNNNNNGC 6 cut(s) 191, 200, 1280, 1589, 1628, 2174
NciI CCSGG 1 cut(s) 1898
NcoI CCATGG 1 cut(s) 141
NdeII GATC 8 cut(s) 259, 574, 728, 770, 1234, 2014, 2114, 2218
NmuCI GTSAC 1 cut(s) 794
NspI RCATGY 4 cut(s) 1148, 1278, 1637, 2282
NspV TTCGAA 1 cut(s) 1513
PaeI GCATGC 1 cut(s) 1148
PagI TCATGA 1 cut(s) 2190
PciI ACATGT 1 cut(s) 1633
PciSI GCTCTTC 1 cut(s) 1650
PcsI WCGNNNNNNNCGW 2 cut(s) 19, 790
PctI GAATGC 1 cut(s) 1249
PfeI GAWTC 2 cut(s) 804, 1834
PfoI TCCNGGA 3 cut(s) 43, 61, 1519
PleI GAGTC 6 cut(s) 13, 239, 967, 1166, 1462, 2095
PpsI GAGTC 6 cut(s) 13, 239, 967, 1166, 1462, 2095
PscI ACATGT 1 cut(s) 1633
PshAI GACNNNNGTC 1 cut(s) 2271
Psp6I CCWGG 8 cut(s) 43, 61, 118, 687, 1237, 1519, 1720, 1768
PspEI GGTNACC 1 cut(s) 2063
PspFI CCCAGC 2 cut(s) 683, 1810
PspGI CCWGG 8 cut(s) 43, 61, 118, 687, 1237, 1519, 1720, 1768
PspOMI GGGCCC 1 cut(s) 691
PsrI GAACNNNNNNTAC 2 cut(s) 121, 153
PstI CTGCAG 2 cut(s) 112, 2176
PsuI RGATCY 2 cut(s) 728, 770
PvuII CAGCTG 2 cut(s) 580, 1814
RsaI GTAC 7 cut(s) 591, 908, 1027, 1042, 1063, 1195, 2097
RsaNI GTAC 7 cut(s) 590, 907, 1026, 1041, 1062, 1194, 2096
SacII CCGCGG 1 cut(s) 1008
SapI GCTCTTC 1 cut(s) 1650
SaqAI TTAA 6 cut(s) 618, 1229, 1347, 1412, 1820, 1911
Sau3AI GATC 8 cut(s) 259, 574, 728, 770, 1234, 2014, 2114, 2218
SchI GAGTC 6 cut(s) 13, 240, 967, 1166, 1462, 2096
ScrFI CCNGG 9 cut(s) 45, 63, 120, 689, 1239, 1521, 1722, 1770, 1898
SduI GDGCHC 5 cut(s) 378, 695, 877, 1897, 2130
SexAI ACCWGGT 1 cut(s) 1720
SfaNI GCATC 7 cut(s) 190, 541, 911, 1253, 1349, 2136, 2296
SfcI CTRYAG 2 cut(s) 108, 2172
Sfr303I CCGCGG 1 cut(s) 1008
SfuI TTCGAA 1 cut(s) 1513
SgrBI CCGCGG 1 cut(s) 1008
SinI GGWCC 2 cut(s) 2185, 2412
SmlI CTYRAG 2 cut(s) 635, 1346
SmoI CTYRAG 2 cut(s) 635, 1346
SpeI ACTAGT 1 cut(s) 1849
SphI GCATGC 1 cut(s) 1148
SspI AATATT 1 cut(s) 1483
SspMI CTAG 9 cut(s) 9, 642, 882, 1056, 1137, 1562, 1737, 1850, 1875
StyD4I CCNGG 9 cut(s) 43, 61, 118, 687, 1237, 1519, 1720, 1768, 1896
StyI CCWWGG 4 cut(s) 141, 1886, 2031, 2262
TaaI ACNGT 6 cut(s) 366, 460, 480, 1045, 1935, 1972
TaiI ACGT 6 cut(s) 91, 1031, 1123, 1199, 1555, 2122
TaqI TCGA 7 cut(s) 262, 1262, 1513, 1951, 2049, 2113, 2221
TfiI GAWTC 2 cut(s) 804, 1834
Tru1I TTAA 6 cut(s) 618, 1229, 1347, 1412, 1820, 1911
Tru9I TTAA 6 cut(s) 618, 1229, 1347, 1412, 1820, 1911
TscAI CASTG 3 cut(s) 526, 1686, 2412
TseFI GTSAC 1 cut(s) 794
Tsp45I GTSAC 1 cut(s) 794
TspDTI ATGAA 4 cut(s) 434, 1855, 2207, 2227
TspGWI ACGGA 1 cut(s) 2195
TspRI CASTG 3 cut(s) 526, 1686, 2412
Vha464I CTTAAG 1 cut(s) 1346
VpaK11BI GGWCC 2 cut(s) 2185, 2412
XapI RAATTY 5 cut(s) 467, 1433, 1691, 2135, 2351
XceI RCATGY 4 cut(s) 1148, 1278, 1637, 2282
XspI CTAG 9 cut(s) 9, 642, 882, 1056, 1137, 1562, 1737, 1850, 1875
ZraI GACGTC 1 cut(s) 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.