FvH4_7g29722

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000094
Physical Location & Seq
Forward (+)
21814557 .. 21816635
2079 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g29722.t1

Sequence Viewer

Length: 2079 bp
ATGAGAAACCTCCAAGTTTCAAACCCATCCTCCACCCACCCCACATCTGCCTTAGTCCCACCCATCCCACCGCCTCGCAACGACAATATCCAGCATCCAAACCGCTCGGCAATACCTCTTTCCACCGACCATTTAGCCACTTTTCAGGCTGCCGTCGATGGCCTTCAATCTCAGTATGATGATGTGCAGCACGCCATCGCGGCGATAGCACGCAGCCAAACTCACATGAATCACCAGTTCGCTGCTATCCAAAACATCATTCTCAATCGGTGGGAAAACCACCCTCCCTCAACAGCACCACAATCCGCTGCTGTTACCCCGTCGTCCGCCCCCATCATCCACAGTTTGGAGCCTTACTCGCCACCTACACCTCCCTCACACCCATTGACCTTTGGGTCCTTCTCTCCGGCGCCAACTACACCTCACCTCACTCTCCCTCAACCCACACTCACTTTATTCACACCTCCCCAACTCACTTATAACACCTCCTCTCACCATTCTCACATGAGTTCAGCGCCACCACAGTACTCGACCATCTCATCACCCCATCCATCCCCTCACCAACAGCACATCAACTACAATCCTCCACCACCAAATCCTCCGAACAACACCAATTTTCCCCCTCCACCAAATCCCATTCCCGAACACCACCACTACCGACCCCCGCGAGTCGAACTACCACATTTCAATGGCGAAGACGTGGTCGGCTGGATAACCTTGGCAGAGCGCTACTTGCGGGTTCATCACGTACCGCCCTTCGATCGCGTTCAAACAGCAGCATTACACTTTGGCAATGACGCTTCAGTTTGGATGAGCGCTTATGAGCTCCGCCACCCCAACCCGACATGGGACGAGTTTGTGGCAGCCCTCATCTCTCGTTTTGGCGGTGGCACTCCGTCCAACTTCAAGAGCGCCCTCTCCCACATACAACAGGCCGGGTCTGTCGACAATTTCATTTTTGCATTCACCAAGCTCTCTTGTCGCGCTCCGGATTGGCCCGACAGTGAACTTGTGCACATCTTCATTAGGGGTCTGCGCCCTGAGCTTCAGCAAGATATCCAAGTGTCCAACCCTACCACCCTAGACGAGGCACAACGCCTTGCACGCTTATTTGAGGCGCGGAACGCTGAGCTTCGCCATTCCCAACCTTACAGACCCCGCACCTTTTCCAACCCACTTCCCAATCGTTTCCACACAACCTCCCTTTCCACTTCACATGCCCCCCCTGCATGCCTACCCCCAACAAACCCCATACCTCCCACTACCAGAACACACCTTCCCACTCCACCACAAAACACCAATCCTCGTTCCCCCTTCCGCCTCCTTTCCACTACGGAACAGAGAGAAAGGCGAGCCAAAAACCTTTACTTCAACTGCGATGAGCAGTATTCCGCCACCCATGTGTGCAAACGGCCATTTATGGCCATCCTCGACTTCGCCCAACCACCTGACGAGTACCCGGAGGTTGACACCGACCCACCGTTGCTCACCGAGGAGCCAACCCTTGATTCCTCCGACCCCCTTTACCCACTTCACGTCATCACCAACACAAAGGTCTGTGATATGATACGTTTCAAGGGCTCCATTGGCAATGTCACCATTAACATCTTCATTGATTGCGGGGCCGCAATGAACTTCCTCAACCCGGCTGTCATCAACAAATTGGGGCTGTCAATTGCCCCCGCCGGGCCTCTTCGATTCACAGCTGCATCGGGCCATTCACTCACTCCTTCCGGGGTCGCACACAACGTCACGGTACAAGCCCAAGCCTACAGTTTCACCGACTCCTTCCTTTTGTTGCCGGTGATTGGCTGCGACCTTATATTGGGGGCACAATGGCTGGAAACCATGGGATTTATTGGTGGGCACTTCCGCGACAAACTCATGGTCTTTGCAGTGGATGGCCAGCGCCATGTTTTGCGCGGCGAAACCTCCAACCCAAAGGCCGACATCGATGCTGCCGACATCTTTGACCTCATCCCACATAAGCAGCTTGAGTCTTTCTCCCCTCCCTTTGCCATTCTAGCCGCAGCTGCGCCACCAGCTCATCCACCAGAGATCACCCTTCTTCTCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

693

Amino Acids

76.41

Weight (kDa)

6.38

Isoelectric Point (pI)

55.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 254 - 369 6.6e-12 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 259 - 347 2.7e-12 Retrotransposon gag protein
gag-asp_proteas PF13975 526 - 615 3.5e-06 gag-polyprotein putative aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000094)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42190
fragaria_vesca FvH4_1g22371 FvH4_1g23261 FvH4_1g27933 FvH4_1g27934 FvH4_1g29231 FvH4_2g02781 FvH4_2g08031 FvH4_2g08581 FvH4_3g06961 FvH4_3g06962 FvH4_3g13531 FvH4_3g14391 FvH4_3g14392 FvH4_3g18622 FvH4_3g18964 FvH4_3g26314 FvH4_3g28781 FvH4_3g30771 FvH4_3g30772 FvH4_3g31752 FvH4_3g32803 FvH4_3g35751 FvH4_3g35752 FvH4_4g02151 FvH4_4g02642 FvH4_4g05523 FvH4_4g05524 FvH4_4g06574 FvH4_4g06722 FvH4_4g08644 FvH4_4g08645 FvH4_4g08646 FvH4_4g08851 FvH4_4g08971 FvH4_4g09081 FvH4_4g09243 FvH4_4g09244 FvH4_4g09283 FvH4_4g10021 FvH4_4g10032 FvH4_4g10033 FvH4_4g10182 FvH4_4g10361 FvH4_4g12502 FvH4_4g12503 FvH4_4g13341 FvH4_4g13942 FvH4_4g13943 FvH4_4g13944 FvH4_4g14432 FvH4_4g14822 FvH4_4g14823 FvH4_4g15291 FvH4_4g15292 FvH4_4g15293 FvH4_4g15471 FvH4_4g15583 FvH4_4g15584 FvH4_4g15585 FvH4_4g15586 FvH4_4g15587 FvH4_4g15591 FvH4_4g15592 FvH4_4g15593 FvH4_4g16179 FvH4_4g18131 FvH4_4g21031 FvH4_4g22021 FvH4_4g22021 FvH4_4g22042 FvH4_5g03402 FvH4_5g07411 FvH4_5g13881 FvH4_5g15792 FvH4_5g24682 FvH4_5g24911 FvH4_5g25322 FvH4_5g25641 FvH4_5g36692 FvH4_5g38072 FvH4_5g38171 FvH4_6g05202 FvH4_6g08531 FvH4_6g16342 FvH4_6g21771 FvH4_6g23303 FvH4_6g23961 FvH4_6g25381 FvH4_6g27021 FvH4_6g27022 FvH4_6g30091 FvH4_6g30092 FvH4_7g02051 FvH4_7g06031 FvH4_7g07031 FvH4_7g07581 FvH4_7g08381 FvH4_7g11551 FvH4_7g19711 FvH4_7g25621 FvH4_7g29722
malus_domestica MD06G1134500.v1.1 MD09G1067600.v1.1 MD13G1145500.v1.1 MD15G1166500.v1.1 MD16G1270000.v1.1
prunus_persica Prupe.7G030900_v2.0.a1
pyrus_communis pycom01g00710 pycom01g03760 pycom03g10770 pycom04g02180 pycom05g08050 pycom06g04140 pycom06g11110 pycom07g07550 pycom07g21570 pycom08g17240 pycom09g13900 pycom09g19570 pycom10g22300 pycom11g03210 pycom11g13750 pycom11g24890 pycom12561g00270 pycom12668g00020 pycom12g11280 pycom12g20720 pycom12g20730 pycom13g21410 pycom13g21660 pycom14g06010 pycom14g06370 pycom14g12800 pycom16g21440 pycom16g22530 pycom17g02030 pycom420g00170 pycom50g00020 pycom5153684g00110 pycom520g00860 pycom581g00060
rosa_chinensis RchiOBHm_Chr0c08g0498091 RchiOBHm_Chr0c08g0498101 RchiOBHm_Chr1g0326991 RchiOBHm_Chr2g0084311 RchiOBHm_Chr2g0090701 RchiOBHm_Chr2g0098591 RchiOBHm_Chr2g0109491 RchiOBHm_Chr2g0109501 RchiOBHm_Chr2g0130801 RchiOBHm_Chr2g0145931 RchiOBHm_Chr2g0168091 RchiOBHm_Chr3g0457081 RchiOBHm_Chr3g0457091 RchiOBHm_Chr3g0467781 RchiOBHm_Chr3g0467791 RchiOBHm_Chr3g0493771 RchiOBHm_Chr3g0494051 RchiOBHm_Chr3g0496881 RchiOBHm_Chr4g0401391 RchiOBHm_Chr4g0414861 RchiOBHm_Chr4g0424081 RchiOBHm_Chr5g0009611 RchiOBHm_Chr5g0049881 RchiOBHm_Chr5g0050351 RchiOBHm_Chr5g0053791 RchiOBHm_Chr5g0078031 RchiOBHm_Chr6g0256481 RchiOBHm_Chr6g0271231 RchiOBHm_Chr6g0286381 RchiOBHm_Chr7g0197101 RchiOBHm_Chr7g0221891 RchiOBHm_Chr7g0237801 RchiOBHm_Chr7g0237821
rosa_multiflora Rmu_sc0000509.1_g000034 Rmu_sc0004751.1_g000014 Rmu_sc0008546.1_g000002 Rmu_sc0009320.1_g000003 Rmu_sc0010661.1_g000006 Rmu_sc0029652.1_g000002
rosa_roxburghii Rroxscaffold_10G00433150 Rroxscaffold_15G00447630 Rroxscaffold_26G00447790 Rroxscaffold_29G00441810 Rroxscaffold_32G00442860 Rroxscaffold_33G00439800 Rroxscaffold_34G00443230 Rroxscaffold_3G00220560 Rroxscaffold_3G00227370 Rroxscaffold_3G00239080 Rroxscaffold_3G00239090 Rroxscaffold_3G00273190 Rroxscaffold_4G00323530 Rroxscaffold_4G00326840 Rroxscaffold_57G00443110 Rroxscaffold_5G00338590 Rroxscaffold_5G00347530 Rroxscaffold_68G00446990 Rroxscaffold_76G00448570 Rroxscaffold_90G00448860
rosa_rugosa Rorug06G0233600
rosa_samantha Rh3CG302700 Rh6DG286700
rosa_wichuraiana Rw0G002280 Rw0G004080 Rw0G011830 Rw1G003690 Rw1G008800 Rw1G009130 Rw1G009140 Rw1G009150 Rw1G009160 Rw1G009410 Rw1G009580 Rw1G012770 Rw1G015850 Rw1G026860 Rw2G003780 Rw2G006480 Rw2G016520 Rw2G016960 Rw2G018170 Rw2G025180 Rw2G025510 Rw2G025610 Rw2G025750 Rw2G025920 Rw2G029950 Rw2G046360 Rw2G046370 Rw2G046380 Rw3G009070 Rw3G025820 Rw3G027340 Rw3G027900 Rw3G028150 Rw3G029060 Rw4G002440 Rw4G005960 Rw4G007520 Rw4G011970 Rw4G011980 Rw4G012190 Rw4G012530 Rw4G018490 Rw4G026380 Rw5G003970 Rw5G030920 Rw5G031780 Rw5G031900 Rw5G033030 Rw5G033040 Rw5G036330 Rw5G039730 Rw5G048110 Rw6G006670 Rw6G007910 Rw6G009720 Rw6G010990 Rw6G012930 Rw6G012940 Rw6G013420 Rw6G014690 Rw6G015290 Rw6G015790 Rw6G015920 Rw6G036510 Rw7G016650 Rw7G023140 Rw7G028650 Rw7G036450 Rw7G036460 Rw7G036910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 480
AasI GACNNNNNNGTC 1 cut(s) 394
AccB1I GGYRCC 1 cut(s) 409
AccB7I CCANNNNNTGG 1 cut(s) 346
AccBSI CCGCTC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 945
AccII CGCG 7 cut(s) 200, 667, 765, 984, 1120, 1875, 1923
AccIII TCCGGA 1 cut(s) 988
AcoI YGGCCR 3 cut(s) 1412, 1422, 1903
AcuI CTGAAG 2 cut(s) 786, 1031
AcyI GRCGYC 1 cut(s) 410
AfaI GTAC 4 cut(s) 527, 750, 1457, 1758
AfeI AGCGCT 2 cut(s) 728, 817
AfiI CCNNNNNNNGG 7 cut(s) 346, 847, 1087, 1645, 1686, 1808, 1825
AgsI TTSAA 7 cut(s) 21, 167, 688, 770, 907, 1372, 1576
AjiI CACGTC 2 cut(s) 700, 1537
AjuI GAANNNNNNNTTGG 4 cut(s) 243, 275, 1492, 1524
AleI CACNNNNGTG 1 cut(s) 1400
AloI GAACNNNNNNTCC 2 cut(s) 1261, 1293
AluBI AGCT 8 cut(s) 826, 973, 1045, 1132, 1706, 1993, 2033, 2045
AluI AGCT 8 cut(s) 826, 973, 1045, 1132, 1706, 1993, 2033, 2045
Alw21I GWGCWC 2 cut(s) 828, 1017
Alw44I GTGCAC 1 cut(s) 1013
Aor13HI TCCGGA 1 cut(s) 988
Aor51HI AGCGCT 2 cut(s) 728, 817
ApaLI GTGCAC 1 cut(s) 1013
ArsI GACNNNNNNTTYG 4 cut(s) 687, 719, 1872, 1904
AspS9I GGNCC 5 cut(s) 396, 996, 1623, 1688, 1714
AsuC2I CCSGG 5 cut(s) 937, 1460, 1646, 1687, 1735
AvaII GGWCC 1 cut(s) 396
BaeGI GKGCMC 3 cut(s) 1017, 1834, 1869
BalI TGGCCA 2 cut(s) 1424, 1905
BanI GGYRCC 1 cut(s) 409
BanII GRGCYC 2 cut(s) 828, 1583
BbsI GAAGAC 1 cut(s) 702
Bbv12I GWGCWC 2 cut(s) 828, 1017
BceAI ACGGC 2 cut(s) 137, 1427
BcgI CGANNNNNNTGC 2 cut(s) 1937, 1971
BcnI CCSGG 5 cut(s) 937, 1460, 1646, 1687, 1735
BfaI CTAG 2 cut(s) 1082, 2024
BfmI CTRYAG 1 cut(s) 1771
BfoI RGCGCY 6 cut(s) 413, 518, 730, 819, 915, 1912
BglI GCCNNNNNGGC 1 cut(s) 200
BlpI GCTNAGC 1 cut(s) 1128
BmcAI AGTACT 1 cut(s) 527
Bme1390I CCNGG 5 cut(s) 937, 1460, 1646, 1687, 1735
Bme18I GGWCC 1 cut(s) 396
BmgBI CACGTC 2 cut(s) 700, 1537
BmgT120I GGNCC 5 cut(s) 396, 996, 1623, 1688, 1714
BmiI GGNNCC 6 cut(s) 351, 397, 411, 1497, 1582, 1624
BmrFI CCNGG 5 cut(s) 937, 1460, 1646, 1687, 1735
BmsI GCATC 3 cut(s) 103, 1718, 1945
BpiI GAAGAC 1 cut(s) 702
BplI GAGNNNNNCTC 4 cut(s) 341, 373, 1988, 2020
BpmI CTGGAG 1 cut(s) 2057
Bpu10I CCTNAGC 1 cut(s) 1041
Bpu1102I GCTNAGC 1 cut(s) 1128
BpuEI CTTGAG 1 cut(s) 2015
BpuMI CCSGG 5 cut(s) 937, 1460, 1646, 1687, 1735
Bsa29I ATCGAT 1 cut(s) 1953
BsaAI YACGTR 1 cut(s) 748
BsaHI GRCGYC 1 cut(s) 410
BsaJI CCNNGG 4 cut(s) 717, 1491, 1734, 1848
BsaWI WCCGGW 1 cut(s) 988
BsaXI ACNNNNNCTCC 4 cut(s) 14, 44, 1184, 1214
Bsc4I CCNNNNNNNGG 7 cut(s) 346, 847, 1087, 1645, 1686, 1808, 1825
Bse118I RCCGGY 1 cut(s) 1801
Bse1I ACTGG 2 cut(s) 235, 2074
Bse3DI GCAATG 3 cut(s) 799, 1597, 1635
BseAI TCCGGA 1 cut(s) 988
BseCI ATCGAT 1 cut(s) 1953
BseDI CCNNGG 4 cut(s) 717, 1491, 1734, 1848
BseLI CCNNNNNNNGG 7 cut(s) 346, 847, 1087, 1645, 1686, 1808, 1825
BseMI GCAATG 3 cut(s) 799, 1597, 1635
BseMII CTCAG 3 cut(s) 185, 1032, 1119
BseNI ACTGG 2 cut(s) 235, 2074
BseRI GAGGAG 2 cut(s) 478, 1508
BseSI GKGCMC 3 cut(s) 1017, 1834, 1869
BsgI GTGCAG 1 cut(s) 206
Bsh1236I CGCG 7 cut(s) 200, 667, 765, 984, 1120, 1875, 1923
Bsh1285I CGRYCG 1 cut(s) 763
BshNI GGYRCC 1 cut(s) 409
BshVI ATCGAT 1 cut(s) 1953
BsiEI CGRYCG 1 cut(s) 763
BsiHKAI GWGCWC 2 cut(s) 828, 1017
BsiSI CCGG 8 cut(s) 407, 936, 989, 1460, 1646, 1686, 1734, 1802
BslFI GGGAC 2 cut(s) 41, 863
BslI CCNNNNNNNGG 7 cut(s) 346, 847, 1087, 1645, 1686, 1808, 1825
BsmFI GGGAC 2 cut(s) 41, 863
BsmI GAATGC 1 cut(s) 962
Bsp1286I GDGCHC 5 cut(s) 828, 1017, 1583, 1834, 1869
Bsp13I TCCGGA 1 cut(s) 988
Bsp143I GATC 2 cut(s) 760, 2058
Bsp1720I GCTNAGC 1 cut(s) 1128
Bsp19I CCATGG 1 cut(s) 1848
BspCNI CTCAG 3 cut(s) 184, 1033, 1120
BspDI ATCGAT 1 cut(s) 1953
BspEI TCCGGA 1 cut(s) 988
BspFNI CGCG 7 cut(s) 200, 667, 765, 984, 1120, 1875, 1923
BspLI GGNNCC 6 cut(s) 351, 397, 411, 1497, 1582, 1624
BspT107I GGYRCC 1 cut(s) 409
BsrBI CCGCTC 1 cut(s) 105
BsrDI GCAATG 3 cut(s) 799, 1597, 1635
BsrFI RCCGGY 1 cut(s) 1801
BsrI ACTGG 2 cut(s) 235, 2074
BssAI RCCGGY 1 cut(s) 1801
BssECI CCNNGG 4 cut(s) 717, 1491, 1734, 1848
BssMI GATC 2 cut(s) 760, 2058
BssNI GRCGYC 1 cut(s) 410
BssT1I CCWWGG 2 cut(s) 717, 1848
Bst4CI ACNGT 6 cut(s) 344, 525, 1004, 1482, 1756, 1775
Bst6I CTCTTC 1 cut(s) 1698
BstACI GRCGYC 1 cut(s) 410
BstBAI YACGTR 1 cut(s) 748
BstC8I GCNNGC 6 cut(s) 192, 211, 1105, 1231, 1353, 1907
BstDEI CTNAG 4 cut(s) 52, 171, 1041, 1128
BstDSI CCRYGG 1 cut(s) 1848
BstENI CCTNNNNNAGG 1 cut(s) 1085
BstFNI CGCG 7 cut(s) 200, 667, 765, 984, 1120, 1875, 1923
BstH2I RGCGCY 6 cut(s) 413, 518, 730, 819, 915, 1912
BstKTI GATC 2 cut(s) 763, 2061
BstMBI GATC 2 cut(s) 760, 2058
BstMCI CGRYCG 1 cut(s) 763
BstNSI RCATGY 2 cut(s) 1220, 1233
BstSCI CCNGG 5 cut(s) 935, 1458, 1644, 1685, 1733
BstSFI CTRYAG 1 cut(s) 1771
BstSLI GKGCMC 3 cut(s) 1017, 1834, 1869
BstUI CGCG 7 cut(s) 200, 667, 765, 984, 1120, 1875, 1923
BstV2I GAAGAC 1 cut(s) 702
Bsu15I ATCGAT 1 cut(s) 1953
BsuTUI ATCGAT 1 cut(s) 1953
BtgI CCRYGG 1 cut(s) 1848
BtgZI GCGATG 2 cut(s) 181, 1392
BtrI CACGTC 2 cut(s) 700, 1537
BtsI GCAGTG 1 cut(s) 1902
BtsIMutI CAGTG 2 cut(s) 1009, 1902
Cac8I GCNNGC 6 cut(s) 192, 211, 1105, 1231, 1353, 1907
Cfr10I RCCGGY 1 cut(s) 1801
Cfr13I GGNCC 5 cut(s) 396, 996, 1623, 1688, 1714
ClaI ATCGAT 1 cut(s) 1953
CseI GACGC 1 cut(s) 806
Csp6I GTAC 4 cut(s) 526, 749, 1456, 1757
CviAII CATG 9 cut(s) 226, 505, 846, 1217, 1230, 1400, 1849, 1885, 1913
CviQI GTAC 4 cut(s) 526, 749, 1456, 1757
DdeI CTNAG 4 cut(s) 52, 171, 1041, 1128
DinI GGCGCC 1 cut(s) 411
DpnI GATC 2 cut(s) 762, 2060
DpnII GATC 2 cut(s) 760, 2058
DrdI GACNNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 1 cut(s) 394
EaeI YGGCCR 3 cut(s) 1412, 1422, 1903
Eam1104I CTCTTC 1 cut(s) 1698
EarI CTCTTC 1 cut(s) 1698
EciI GGCGGA 4 cut(s) 316, 818, 1307, 1381
Ecl136II GAGCTC 1 cut(s) 826
Eco130I CCWWGG 2 cut(s) 717, 1848
Eco24I GRGCYC 2 cut(s) 828, 1583
Eco32I GATATC 1 cut(s) 1057
Eco47I GGWCC 1 cut(s) 396
Eco47III AGCGCT 2 cut(s) 728, 817
Eco53kI GAGCTC 1 cut(s) 826
Eco57I CTGAAG 2 cut(s) 786, 1031
EcoICRI GAGCTC 1 cut(s) 826
EcoNI CCTNNNNNAGG 1 cut(s) 1085
EcoO109I RGGNCCY 1 cut(s) 396
EcoRV GATATC 1 cut(s) 1057
EcoT14I CCWWGG 2 cut(s) 717, 1848
EcoT38I GRGCYC 2 cut(s) 828, 1583
EgeI GGCGCC 1 cut(s) 411
EheI GGCGCC 1 cut(s) 411
ErhI CCWWGG 2 cut(s) 717, 1848
FaeI CATG 9 cut(s) 229, 508, 849, 1220, 1233, 1403, 1852, 1888, 1916
FaqI GGGAC 2 cut(s) 41, 863
FatI CATG 9 cut(s) 225, 504, 845, 1216, 1229, 1399, 1848, 1884, 1912
FauI CCCGC 5 cut(s) 672, 729, 1166, 1613, 1690
FblI GTMKAC 1 cut(s) 945
FriOI GRGCYC 2 cut(s) 828, 1583
FspBI CTAG 2 cut(s) 1082, 2024
GsuI CTGGAG 1 cut(s) 2057
HaeII RGCGCY 6 cut(s) 413, 518, 730, 819, 915, 1912
HapII CCGG 8 cut(s) 407, 936, 989, 1460, 1646, 1686, 1734, 1802
HgaI GACGC 1 cut(s) 806
Hin1I GRCGYC 1 cut(s) 410
Hin1II CATG 9 cut(s) 229, 508, 849, 1220, 1233, 1403, 1852, 1888, 1916
HincII GTYRAC 2 cut(s) 946, 1468
HindII GTYRAC 2 cut(s) 946, 1468
HinfI GANTC 6 cut(s) 229, 669, 1508, 1698, 1784, 1997
HpaII CCGG 8 cut(s) 407, 936, 989, 1460, 1646, 1686, 1734, 1802
Hpy166II GTNNAC 4 cut(s) 946, 1007, 1015, 1468
Hpy188I TCNGA 2 cut(s) 603, 1516
Hpy188III TCNNGA 3 cut(s) 641, 907, 989
Hpy8I GTNNAC 4 cut(s) 946, 1007, 1015, 1468
Hpy99I CGWCG 2 cut(s) 158, 325
HpyAV CCTTC 8 cut(s) 173, 409, 766, 1286, 1324, 1740, 1798, 2075
HpyCH4III ACNGT 6 cut(s) 344, 525, 1004, 1482, 1756, 1775
HpyCH4IV ACGT 5 cut(s) 699, 747, 1536, 1570, 1749
HpyCH4V TGCA 8 cut(s) 187, 962, 1015, 1103, 1229, 1407, 1709, 1895
HpyF3I CTNAG 4 cut(s) 52, 171, 1041, 1128
HpySE526I ACGT 5 cut(s) 699, 747, 1536, 1570, 1749
Hsp92I GRCGYC 1 cut(s) 410
Hsp92II CATG 9 cut(s) 229, 508, 849, 1220, 1233, 1403, 1852, 1888, 1916
KasI GGCGCC 1 cut(s) 409
Kpn2I TCCGGA 1 cut(s) 988
Kzo9I GATC 2 cut(s) 760, 2058
LmnI GCTCC 5 cut(s) 349, 831, 991, 1495, 1586
LweI GCATC 3 cut(s) 103, 1718, 1945
MaeI CTAG 2 cut(s) 1082, 2024
MaeII ACGT 5 cut(s) 699, 747, 1536, 1570, 1749
MaeIII GTNAC 3 cut(s) 313, 1594, 1750
MalI GATC 2 cut(s) 762, 2060
MbiI CCGCTC 1 cut(s) 105
MboI GATC 2 cut(s) 760, 2058
MboII GAAGA 5 cut(s) 707, 1012, 1600, 1685, 2060
MfeI CAATTG 1 cut(s) 1674
MhlI GDGCHC 5 cut(s) 828, 1017, 1583, 1834, 1869
MlsI TGGCCA 2 cut(s) 1424, 1905
MluCI AATT 4 cut(s) 613, 949, 1661, 1674
MluNI TGGCCA 2 cut(s) 1424, 1905
Mly113I GGCGCC 1 cut(s) 410
MlyI GAGTC 3 cut(s) 678, 1778, 2006
MmeI TCCRAC 5 cut(s) 924, 1092, 1194, 1539, 1959
Mox20I TGGCCA 2 cut(s) 1424, 1905
MroI TCCGGA 1 cut(s) 988
MscI TGGCCA 2 cut(s) 1424, 1905
MseI TTAA 1 cut(s) 1602
MslI CAYNNNNRTG 2 cut(s) 687, 1400
Msp20I TGGCCA 2 cut(s) 1424, 1905
MspA1I CMGCKG 3 cut(s) 308, 1706, 2033
MspI CCGG 8 cut(s) 407, 936, 989, 1460, 1646, 1686, 1734, 1802
MspR9I CCNGG 5 cut(s) 937, 1460, 1646, 1687, 1735
MunI CAATTG 1 cut(s) 1674
Mva1269I GAATGC 1 cut(s) 962
MvnI CGCG 7 cut(s) 200, 667, 765, 984, 1120, 1875, 1923
NarI GGCGCC 1 cut(s) 410
NciI CCSGG 5 cut(s) 937, 1460, 1646, 1687, 1735
NcoI CCATGG 1 cut(s) 1848
NdeII GATC 2 cut(s) 760, 2058
NlaIII CATG 9 cut(s) 229, 508, 849, 1220, 1233, 1403, 1852, 1888, 1916
NlaIV GGNNCC 6 cut(s) 351, 397, 411, 1497, 1582, 1624
NmeAIII GCCGAG 1 cut(s) 86
NmuCI GTSAC 2 cut(s) 1594, 1750
NspI RCATGY 2 cut(s) 1220, 1233
OliI CACNNNNGTG 1 cut(s) 1400
PaeI GCATGC 1 cut(s) 1233
PcsI WCGNNNNNNNCGW 2 cut(s) 1746, 1959
PctI GAATGC 1 cut(s) 962
PfeI GAWTC 3 cut(s) 229, 1508, 1698
PflFI GACNNNGTC 1 cut(s) 701
PflMI CCANNNNNTGG 1 cut(s) 346
Ple19I CGATCG 1 cut(s) 763
PleI GAGTC 3 cut(s) 677, 1778, 2005
PluTI GGCGCC 1 cut(s) 413
PpsI GAGTC 3 cut(s) 677, 1778, 2005
Ppu21I YACGTR 1 cut(s) 748
PpuMI RGGWCCY 1 cut(s) 396
PsiI TTATAA 1 cut(s) 480
Psp124BI GAGCTC 1 cut(s) 828
Psp5II RGGWCCY 1 cut(s) 396
PspN4I GGNNCC 6 cut(s) 351, 397, 411, 1497, 1582, 1624
PspPI GGNCC 5 cut(s) 396, 996, 1623, 1688, 1714
PspPPI RGGWCCY 1 cut(s) 396
PsyI GACNNNGTC 1 cut(s) 701
PvuI CGATCG 1 cut(s) 763
PvuII CAGCTG 2 cut(s) 1706, 2033
RsaI GTAC 4 cut(s) 527, 750, 1457, 1758
RsaNI GTAC 4 cut(s) 526, 749, 1456, 1757
RseI CAYNNNNRTG 2 cut(s) 687, 1400
SacI GAGCTC 1 cut(s) 828
SalI GTCGAC 1 cut(s) 944
SaqAI TTAA 1 cut(s) 1602
Sau3AI GATC 2 cut(s) 760, 2058
Sau96I GGNCC 5 cut(s) 396, 996, 1623, 1688, 1714
ScaI AGTACT 1 cut(s) 527
SchI GAGTC 3 cut(s) 678, 1778, 2006
ScrFI CCNGG 5 cut(s) 937, 1460, 1646, 1687, 1735
SduI GDGCHC 5 cut(s) 828, 1017, 1583, 1834, 1869
SfaNI GCATC 3 cut(s) 103, 1718, 1945
SfcI CTRYAG 1 cut(s) 1771
SfoI GGCGCC 1 cut(s) 411
SinI GGWCC 1 cut(s) 396
SmiMI CAYNNNNRTG 2 cut(s) 687, 1400
SmlI CTYRAG 1 cut(s) 1994
SmoI CTYRAG 1 cut(s) 1994
SphI GCATGC 1 cut(s) 1233
Sse9I AATT 4 cut(s) 613, 949, 1661, 1674
SspDI GGCGCC 1 cut(s) 409
SspMI CTAG 2 cut(s) 1082, 2024
SstI GAGCTC 1 cut(s) 828
StyD4I CCNGG 5 cut(s) 935, 1458, 1644, 1685, 1733
StyI CCWWGG 2 cut(s) 717, 1848
TaaI ACNGT 6 cut(s) 344, 525, 1004, 1482, 1756, 1775
TaiI ACGT 5 cut(s) 702, 750, 1539, 1573, 1752
TaqI TCGA 8 cut(s) 156, 530, 672, 759, 945, 1431, 1696, 1953
TasI AATT 4 cut(s) 613, 949, 1661, 1674
TatI WGTACW 1 cut(s) 525
TauI GCSGC 4 cut(s) 203, 1628, 1926, 2030
TfiI GAWTC 3 cut(s) 229, 1508, 1698
Tru1I TTAA 1 cut(s) 1602
Tru9I TTAA 1 cut(s) 1602
TscAI CASTG 2 cut(s) 1009, 1902
TseFI GTSAC 2 cut(s) 1594, 1750
Tsp45I GTSAC 2 cut(s) 1594, 1750
TspDTI ATGAA 6 cut(s) 242, 731, 943, 1012, 1600, 1646
TspGWI ACGGA 2 cut(s) 885, 1349
TspRI CASTG 2 cut(s) 1009, 1902
Tth111I GACNNNGTC 1 cut(s) 701
Van91I CCANNNNNTGG 1 cut(s) 346
VneI GTGCAC 1 cut(s) 1013
VpaK11BI GGWCC 1 cut(s) 396
XagI CCTNNNNNAGG 1 cut(s) 1085
XceI RCATGY 2 cut(s) 1220, 1233
XmiI GTMKAC 1 cut(s) 945
XspI CTAG 2 cut(s) 1082, 2024
ZrmI AGTACT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.