FvH4_7g06031

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000094
Physical Location & Seq
Reverse (-)
6216032 .. 6220245
4214 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g06031.t1

Sequence Viewer

Length: 3648 bp
ATGTCGTGGGAGCAGTTTAAGACTCTCTTCTTGGGGCAGTATTTCAGTCCTGCCCACCGCAGCAGAATTCAGTCAGATTTCCTGAATATGAAGAAGGGGGATGATGAAGGCGTTATAGAGTTCCAGGAGAGATTCACTTCCCTGAGCTATCATGTGTCGTATTTGGTTCCTGATGAGAGGACCAGGATGGAGATGTTCATTGGGGCTCTTGGTGGAGTGTTTGCAGATAAGATGACGGGTGTTTATTACCCGACTTTTTCTGACGCTGTTCATGCAGCCATGGCCATTGAGTCTATGGGGATTTATAGTGCTCGGCCACGAGATTTCGGTGGTCCCAGTCAGGGTCCATCCAAGAAAGCTGCTTCATCATCCGCTTCAGGTTCATCAGCTGGTAGTGGCAGTAGTGGGGGATCAGGTTCTCATTCTCGGTTCAGGGTTAGAGGACGTCACAGGAGACCTGCTAGGAGCCAGTTGGGTAGGCAGCAGTCTGGGCAGTATGGTTCGGGTGGCAGTTTCCATGGTGGGTCATCCGGTAGCCAGACTTTTTCATATGGGCAGCAGCAGCAGTTTTCTGGGTGCTTTGAGTGTGGTGGTCAGGACCACTTCAGGAGAGATTGCCCATTGTTGGCTCAGAGGACCACTCCCACCCCTACTCAGCCTATTGGTCAGTCTTCAGCTGGAGGCTCTAGTAGTGGTGCCCAGGGCAGTGCTTCGGTCAGAGGTGGCTCCCAGCGGGGAAGTGGTCAGCGTGGACGTCCCATGACTCGCGCTAGACTGCATGCTATGACCCAGCAGGAGGGTCGTACTTCTCCAGATGTTATCATTGGTACGTTATTGATTTTTGGTCAGCCTGCTTTTACTTTAATTGATCCCGGAGCCACACATTCATTCATGTCTAGTCGATTTGCCCTCCATGCCAATGTGCCATCCTCGTCTCTTCCAGGCGAGTGGCATGTGTCTCTACCCTCTGGTGAGGTGATGAGAATAAATTGGGTATTTAGTGGCTGTGAAGTGGTAGTGGAAGGAGTTAATTTCGAGGCTGATTTAATTCCTTTAGATATAGTGGAGTTTGATGTGATCTTGGGAATGGACTTTTTGGAAGCATATAGAGCTATGGTAGATTGTTTTCAAAAAGTAGTGGTGCTTCGAAGTCCAGATGGTTTTGAGGTTGCTTTCCAAGGGGAAAGAGATGTTATTTTTAGTTGCATAATTTCCGCTGTTGCTGCAAGGAAATTGTTAAGTAAAGGCTGTCAGGCATTTTTAGCTCATGTGGTGGATACAAGTATGGGAGTTGTGGGATTAGAAGATATTCCTGTAGTAAGAGAGTTTCCGGATGTTTTTCCTGAGGAATTGCCTGGTTTGCCTCCTGCAAGGGAGATAGACTTCTCTATAGAATTACTTCCCGGAACTATGCCGATCTCTCAGGCGCCTTACAGAATGGCTCCAGCGGAATTGAAAGAGTTGAAGACTCAATTGCAGGAGTTGGTAGATAAGGGTTTTATTCGGCCTAGCATGTCTCCATGGGGTGCTCCGGTGTTGTTTGTTAAGAAGAAAGATGGCACCATGAGACTATGCATTGATTATAGGAAGCTGAATCAGGTCACAGTGAAGAATAAATATCCGTTGCCTCGGATCGATGATTTATTTGATCAGTTGAGGGGTGCCTCTGTTTTCTCCAAGATTGATTTGAGATCGGGGTATCATCAGTTGCGGATCCGAGAGGGGGACATAGCCAAGACTGCTTTTCGGTCACGATATGGTCATTATGAATTTGTGGTGATGCCTTTTGGACTTACTAATGCACCCGCGGCGTTCATGGATCTCATGAATAGGGTGTTTAGTCCATATCTTGATCGTTTCGTGATCGTATTCATAGATGATATCTTGGTTTACTCAAGAAGTGAGAAGGAGCATGCCAAGCATCTAAGAATAATATTGCGGACTTTGGAAAAGTCGCAATTATTTGCTAAGTTTAGCAAGTGTGAGTTTTGGTTAGATAAAGTGGGGTTCTTGGGTCATGTGATTTCAGCGGAAGGAGTTAGTGTGGATCCACAAAAGGTGGAAGCAGTGTCGAATTGGAGAAGACCCACCACTGTGACGGAGATTCGTAGTTTCTTGGGATTAGCAGGTTATTATCGGCGCTTTGTGAAAGATTTTTCAAAGATTGCTGCTCCTCTCACAAAGTTGACCAGAAAGGGGGTTAAGTTTGAATGGTCAGATCAGTGCGAGCAAAGTTTTCAAGAATTGAAGAATTGCTTGACAAGTGCTCCTGTTTTGGCACTTCCGGATGATAGTGGAGAATATGTGGTCTATTGTGATGCTTCTAGACGTGGTCTAGGTGGAGTTTTGATGCAGCATGATCGAGTTATTGCCTACGCTTCTAGGCAATTGAAGCCGCATGAGGGGAATTATCCTACTCATGATCTGGAGCTTGCAGCAGTAGTTTTTGCTCTCAAGTTGTGGAGACACTATCTTTATGGAGCCAAGTGTCAGATATTTACCGATCACAAGAGTCTTCGAAATGAAGAGTTGAAGAGTGAGATTATGGAGGAAGCTCATAGCTCTGCATATGCTGCTCATCCGGGTAGCACAAAAATGTATCGGATTTTGAAGGATTATTATTGGTGGCCAAACATGAAGAGGGAAATTGCAGCTTATGTGAGTAGATGTCTGATTTGTCAGCAAGTCAAGGCTGAAAGGCAGAAGCCTTCAGGATTGCTTGAGCCGTTGCCCATTCCAGTGTGGAAGTGGGACCACATCACCATGGATTTTGTTTTTAGCTTACCTCGTACCCGTGATGGGCATGACGGTATTTGGGTGATTGTGGATAGACTCACCAAGTCTGCACATTTCTTGCCAGTGGGAAAGAAGTATAAATTGGATAAATTGGCGGAGCTCTATGTGAATGAAATTGTGAGGCTTCATGGTGTTCCTGAATCAATAGTGTCAGATCGGGACCCTCGATTTGCTTCTAAATTCTGGGGAGCTCTTCAGGTAGCTTTAGGTACCCAATTACTCTTCAGCACTGCTTTTCATCCTCAGACTGATGGGCAGTCCGAGAGGACTATTCAGACTTTGGAGGATATGTTGAGGGCTTGTGCCATGCAGTTCAGAGAGTTAATCCATGTTACGAATGAGAAGATTAAAGTGGTTAGAGATAGACTCAAGACCGCTCAGAGTAGGCAGAAGAGTTATGCAGATGTCCGTAGGAGAGATCTTGAATTTCAAGTGGGTGATTGGGTGTTCCTGAAATTATCTCCTTGGAAAGGAGTTGTGCGTTTCGGTAAGCGTGGGAAGCTTAGTCCGAGGTATATTGGCCCTTATGAGATTATAGAGCGAGTTGGTTCACTTGCTTATCGATTGGCCTTGCCTCCAGAGTTATCCCGGATACATAATGTGTTTCATGTATCCATGCTTCGCAAGTATATTGCCGATCCTTCTCATGTGCTGCAAGAGCAGCCGATCAGTTTGACGAGAGATTTGACCTATGAAGAGGAACCGGTTCAGATTCTCGACCGAAAGGAGCAGGTGCTTAGGAACAAGACAATCCCGCTTGTCAAGGTGCTGTGGAGAAGTCATCAAGTAGAAGAAGCTACTTGGGAATCAGAGGAGCAAATGATGCAGCAGTATCCCTATCTCTTCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve zf-CCHC

Protein Analysis

1216

Amino Acids

137.16

Weight (kDa)

9.06

Isoelectric Point (pI)

44.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 2 - 69 1.3e-07 Retrotransposon gag protein
RVP_2 PF08284 254 - 384 1.3e-33 Retroviral aspartyl protease
RVT_1 PF00078 515 - 675 6.6e-36 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 738 - 832 3.3e-32 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 764 - 840 2.4e-25 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 841 - 898 3.3e-20 Integrase zinc binding domain
SH3_Tf2-1 PF24626 1078 - 1142 5e-28 Tf2-1-like, SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000094)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42190
fragaria_vesca FvH4_1g22371 FvH4_1g23261 FvH4_1g27933 FvH4_1g27934 FvH4_1g29231 FvH4_2g02781 FvH4_2g08031 FvH4_2g08581 FvH4_3g06961 FvH4_3g06962 FvH4_3g13531 FvH4_3g14391 FvH4_3g14392 FvH4_3g18622 FvH4_3g18964 FvH4_3g26314 FvH4_3g28781 FvH4_3g30771 FvH4_3g30772 FvH4_3g31752 FvH4_3g32803 FvH4_3g35751 FvH4_3g35752 FvH4_4g02151 FvH4_4g02642 FvH4_4g05523 FvH4_4g05524 FvH4_4g06574 FvH4_4g06722 FvH4_4g08644 FvH4_4g08645 FvH4_4g08646 FvH4_4g08851 FvH4_4g08971 FvH4_4g09081 FvH4_4g09243 FvH4_4g09244 FvH4_4g09283 FvH4_4g10021 FvH4_4g10032 FvH4_4g10033 FvH4_4g10182 FvH4_4g10361 FvH4_4g12502 FvH4_4g12503 FvH4_4g13341 FvH4_4g13942 FvH4_4g13943 FvH4_4g13944 FvH4_4g14432 FvH4_4g14822 FvH4_4g14823 FvH4_4g15291 FvH4_4g15292 FvH4_4g15293 FvH4_4g15471 FvH4_4g15583 FvH4_4g15584 FvH4_4g15585 FvH4_4g15586 FvH4_4g15587 FvH4_4g15591 FvH4_4g15592 FvH4_4g15593 FvH4_4g16179 FvH4_4g18131 FvH4_4g21031 FvH4_4g22021 FvH4_4g22021 FvH4_4g22042 FvH4_5g03402 FvH4_5g07411 FvH4_5g13881 FvH4_5g15792 FvH4_5g24682 FvH4_5g24911 FvH4_5g25322 FvH4_5g25641 FvH4_5g36692 FvH4_5g38072 FvH4_5g38171 FvH4_6g05202 FvH4_6g08531 FvH4_6g16342 FvH4_6g21771 FvH4_6g23303 FvH4_6g23961 FvH4_6g25381 FvH4_6g27021 FvH4_6g27022 FvH4_6g30091 FvH4_6g30092 FvH4_7g02051 FvH4_7g06031 FvH4_7g07031 FvH4_7g07581 FvH4_7g08381 FvH4_7g11551 FvH4_7g19711 FvH4_7g25621 FvH4_7g29722
malus_domestica MD06G1134500.v1.1 MD09G1067600.v1.1 MD13G1145500.v1.1 MD15G1166500.v1.1 MD16G1270000.v1.1
prunus_persica Prupe.7G030900_v2.0.a1
pyrus_communis pycom01g00710 pycom01g03760 pycom03g10770 pycom04g02180 pycom05g08050 pycom06g04140 pycom06g11110 pycom07g07550 pycom07g21570 pycom08g17240 pycom09g13900 pycom09g19570 pycom10g22300 pycom11g03210 pycom11g13750 pycom11g24890 pycom12561g00270 pycom12668g00020 pycom12g11280 pycom12g20720 pycom12g20730 pycom13g21410 pycom13g21660 pycom14g06010 pycom14g06370 pycom14g12800 pycom16g21440 pycom16g22530 pycom17g02030 pycom420g00170 pycom50g00020 pycom5153684g00110 pycom520g00860 pycom581g00060
rosa_chinensis RchiOBHm_Chr0c08g0498091 RchiOBHm_Chr0c08g0498101 RchiOBHm_Chr1g0326991 RchiOBHm_Chr2g0084311 RchiOBHm_Chr2g0090701 RchiOBHm_Chr2g0098591 RchiOBHm_Chr2g0109491 RchiOBHm_Chr2g0109501 RchiOBHm_Chr2g0130801 RchiOBHm_Chr2g0145931 RchiOBHm_Chr2g0168091 RchiOBHm_Chr3g0457081 RchiOBHm_Chr3g0457091 RchiOBHm_Chr3g0467781 RchiOBHm_Chr3g0467791 RchiOBHm_Chr3g0493771 RchiOBHm_Chr3g0494051 RchiOBHm_Chr3g0496881 RchiOBHm_Chr4g0401391 RchiOBHm_Chr4g0414861 RchiOBHm_Chr4g0424081 RchiOBHm_Chr5g0009611 RchiOBHm_Chr5g0049881 RchiOBHm_Chr5g0050351 RchiOBHm_Chr5g0053791 RchiOBHm_Chr5g0078031 RchiOBHm_Chr6g0256481 RchiOBHm_Chr6g0271231 RchiOBHm_Chr6g0286381 RchiOBHm_Chr7g0197101 RchiOBHm_Chr7g0221891 RchiOBHm_Chr7g0237801 RchiOBHm_Chr7g0237821
rosa_multiflora Rmu_sc0000509.1_g000034 Rmu_sc0004751.1_g000014 Rmu_sc0008546.1_g000002 Rmu_sc0009320.1_g000003 Rmu_sc0010661.1_g000006 Rmu_sc0029652.1_g000002
rosa_roxburghii Rroxscaffold_10G00433150 Rroxscaffold_15G00447630 Rroxscaffold_26G00447790 Rroxscaffold_29G00441810 Rroxscaffold_32G00442860 Rroxscaffold_33G00439800 Rroxscaffold_34G00443230 Rroxscaffold_3G00220560 Rroxscaffold_3G00227370 Rroxscaffold_3G00239080 Rroxscaffold_3G00239090 Rroxscaffold_3G00273190 Rroxscaffold_4G00323530 Rroxscaffold_4G00326840 Rroxscaffold_57G00443110 Rroxscaffold_5G00338590 Rroxscaffold_5G00347530 Rroxscaffold_68G00446990 Rroxscaffold_76G00448570 Rroxscaffold_90G00448860
rosa_rugosa Rorug06G0233600
rosa_samantha Rh3CG302700 Rh6DG286700
rosa_wichuraiana Rw0G002280 Rw0G004080 Rw0G011830 Rw1G003690 Rw1G008800 Rw1G009130 Rw1G009140 Rw1G009150 Rw1G009160 Rw1G009410 Rw1G009580 Rw1G012770 Rw1G015850 Rw1G026860 Rw2G003780 Rw2G006480 Rw2G016520 Rw2G016960 Rw2G018170 Rw2G025180 Rw2G025510 Rw2G025610 Rw2G025750 Rw2G025920 Rw2G029950 Rw2G046360 Rw2G046370 Rw2G046380 Rw3G009070 Rw3G025820 Rw3G027340 Rw3G027900 Rw3G028150 Rw3G029060 Rw4G002440 Rw4G005960 Rw4G007520 Rw4G011970 Rw4G011980 Rw4G012190 Rw4G012530 Rw4G018490 Rw4G026380 Rw5G003970 Rw5G030920 Rw5G031780 Rw5G031900 Rw5G033030 Rw5G033040 Rw5G036330 Rw5G039730 Rw5G048110 Rw6G006670 Rw6G007910 Rw6G009720 Rw6G010990 Rw6G012930 Rw6G012940 Rw6G013420 Rw6G014690 Rw6G015290 Rw6G015790 Rw6G015920 Rw6G036510 Rw7G016650 Rw7G023140 Rw7G028650 Rw7G036450 Rw7G036460 Rw7G036910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3517
AatII GACGTC 2 cut(s) 448, 757
Acc36I ACCTGC 3 cut(s) 466, 2117, 3517
Acc65I GGTACC 1 cut(s) 3005
AccB1I GGYRCC 5 cut(s) 695, 1426, 1559, 1661, 3005
AccBSI CCGCTC 1 cut(s) 3173
AccII CGCG 2 cut(s) 768, 1808
AccIII TCCGGA 2 cut(s) 1330, 2284
AclWI GGATC 9 cut(s) 418, 863, 1640, 1708, 1721, 1827, 2042, 2055, 3428
AcoI YGGCCR 3 cut(s) 282, 314, 2627
AcsI RAATTY 4 cut(s) 66, 1769, 2975, 3221
AcuI CTGAAG 6 cut(s) 360, 589, 657, 2694, 2975, 3004
AcyI GRCGYC 3 cut(s) 445, 754, 1427
AfaI GTAC 4 cut(s) 805, 829, 2791, 3007
AfiI CCNNNNNNNGG 8 cut(s) 341, 625, 796, 1723, 2196, 2402, 2799, 3266
AgeI ACCGGT 1 cut(s) 3499
AjiI CACGTC 1 cut(s) 2330
AjnI CCWGG 5 cut(s) 123, 182, 699, 940, 1354
AjuI GAANNNNNNNTTGG 2 cut(s) 2861, 2893
AleI CACNNNNGTG 1 cut(s) 2093
Alw21I GWGCWC 5 cut(s) 313, 1531, 2269, 2898, 2989
Alw26I GTCTC 6 cut(s) 448, 939, 963, 1521, 1561, 2458
AlwI GGATC 9 cut(s) 418, 863, 1640, 1708, 1721, 1827, 2042, 2055, 3428
Aor13HI TCCGGA 2 cut(s) 1330, 2284
AoxI GGCC 6 cut(s) 282, 314, 1505, 2627, 3316, 3363
ApoI RAATTY 4 cut(s) 66, 1769, 2975, 3221
ArsI GACNNNNNNTTYG 4 cut(s) 2948, 2980, 3463, 3495
AsiGI ACCGGT 1 cut(s) 3499
Asp700I GAANNNNTTC 3 cut(s) 1308, 1398, 3501
Asp718I GGTACC 1 cut(s) 3005
AspLEI GCGC 3 cut(s) 770, 1429, 2142
AspS9I GGNCC 8 cut(s) 180, 332, 344, 598, 636, 2752, 2956, 3317
AsuC2I CCSGG 4 cut(s) 873, 1404, 2583, 3385
AsuHPI GGTGA 7 cut(s) 983, 988, 1789, 2752, 2827, 2830, 3245
AsuII TTCGAA 2 cut(s) 1147, 2518
AvaII GGWCC 7 cut(s) 180, 332, 344, 598, 636, 2752, 2956
AxyI CCTNAGG 1 cut(s) 1344
BaeGI GKGCMC 1 cut(s) 700
BalI TGGCCA 2 cut(s) 284, 2629
BamHI GGATCC 2 cut(s) 1713, 2047
BanI GGYRCC 5 cut(s) 695, 1426, 1559, 1661, 3005
BanII GRGCYC 3 cut(s) 208, 2898, 2989
BarI GAAGNNNNNNTAC 4 cut(s) 1128, 1160, 3399, 3431
BauI CACGAG 1 cut(s) 318
BbsI GAAGAC 4 cut(s) 663, 1472, 2089, 2507
Bbv12I GWGCWC 5 cut(s) 313, 1531, 2269, 2898, 2989
BccI CCATC 7 cut(s) 181, 355, 934, 1151, 1550, 2792, 3041
BceAI ACGGC 1 cut(s) 2710
BcgI CGANNNNNNTGC 4 cut(s) 782, 816, 2342, 2376
BciT130I CCWGG 5 cut(s) 125, 184, 701, 942, 1356
BciVI GTATCC 4 cut(s) 1270, 3381, 3418, 3639
BclI TGATCA 1 cut(s) 1648
BcnI CCSGG 4 cut(s) 873, 1404, 2583, 3385
BcoDI GTCTC 6 cut(s) 448, 939, 963, 1521, 1561, 2458
BfaI CTAG 8 cut(s) 462, 687, 771, 897, 1509, 2325, 2336, 2382
BfmI CTRYAG 2 cut(s) 1314, 1389
BfoI RGCGCY 2 cut(s) 1430, 2143
BfuAI ACCTGC 3 cut(s) 466, 2117, 3517
BfuI GTATCC 4 cut(s) 1270, 3381, 3418, 3639
BglII AGATCT 1 cut(s) 3214
Bme1390I CCNGG 9 cut(s) 125, 184, 701, 873, 942, 1356, 1404, 2583, 3385
Bme18I GGWCC 7 cut(s) 180, 332, 344, 598, 636, 2752, 2956
BmgBI CACGTC 1 cut(s) 2330
BmgT120I GGNCC 8 cut(s) 180, 332, 344, 598, 636, 2752, 2956, 3317
BmrFI CCNGG 9 cut(s) 125, 184, 701, 873, 942, 1356, 1404, 2583, 3385
BmrI ACTGGG 1 cut(s) 330
BmsI GCATC 5 cut(s) 1770, 1930, 2308, 2340, 3609
BmuI ACTGGG 1 cut(s) 330
BpiI GAAGAC 4 cut(s) 663, 1472, 2089, 2507
BplI GAGNNNNNCTC 4 cut(s) 625, 657, 3147, 3179
BpmI CTGGAG 5 cut(s) 699, 795, 1428, 2447, 3357
Bpu10I CCTNAGC 2 cut(s) 143, 3533
Bpu14I TTCGAA 2 cut(s) 1147, 2518
BpuEI CTTGAG 4 cut(s) 1879, 2438, 2741, 3149
BpuMI CCSGG 4 cut(s) 873, 1404, 2583, 3385
Bsa29I ATCGAT 2 cut(s) 1635, 3358
BsaHI GRCGYC 3 cut(s) 445, 754, 1427
BsaI GGTCTC 1 cut(s) 448
BsaWI WCCGGW 5 cut(s) 530, 1330, 1531, 2284, 3499
BsaXI ACNNNNNCTCC 8 cut(s) 2028, 2058, 2251, 2281, 2289, 2319, 3071, 3101
Bsc4I CCNNNNNNNGG 8 cut(s) 341, 625, 796, 1723, 2196, 2402, 2799, 3266
Bse118I RCCGGY 1 cut(s) 3499
Bse1I ACTGG 4 cut(s) 336, 469, 2738, 2858
Bse21I CCTNAGG 1 cut(s) 1344
BseAI TCCGGA 2 cut(s) 1330, 2284
BseBI CCWGG 5 cut(s) 125, 184, 701, 942, 1356
BseCI ATCGAT 2 cut(s) 1635, 3358
BseLI CCNNNNNNNGG 8 cut(s) 341, 625, 796, 1723, 2196, 2402, 2799, 3266
BseMII CTCAG 7 cut(s) 134, 644, 668, 1335, 1436, 3053, 3188
BseNI ACTGG 4 cut(s) 336, 469, 2738, 2858
BseRI GAGGAG 2 cut(s) 2163, 3623
BseSI GKGCMC 1 cut(s) 700
BseYI CCCAGC 2 cut(s) 729, 789
BsgI GTGCAG 1 cut(s) 2829
Bsh1236I CGCG 2 cut(s) 768, 1808
Bsh1285I CGRYCG 1 cut(s) 3517
BshFI GGCC 6 cut(s) 284, 316, 1507, 2629, 3318, 3365
BshNI GGYRCC 5 cut(s) 695, 1426, 1559, 1661, 3005
BshTI ACCGGT 1 cut(s) 3499
BshVI ATCGAT 2 cut(s) 1635, 3358
BsiEI CGRYCG 1 cut(s) 3517
BsiHKAI GWGCWC 5 cut(s) 313, 1531, 2269, 2898, 2989
BsiSI CCGG 9 cut(s) 531, 873, 1331, 1404, 1532, 2285, 2582, 3385, 3500
BslFI GGGAC 5 cut(s) 318, 741, 1739, 2765, 2969
BslI CCNNNNNNNGG 8 cut(s) 341, 625, 796, 1723, 2196, 2402, 2799, 3266
BsmAI GTCTC 6 cut(s) 448, 939, 963, 1521, 1561, 2458
BsmBI CGTCTC 1 cut(s) 939
BsmFI GGGAC 5 cut(s) 318, 741, 1739, 2765, 2969
BsnI GGCC 6 cut(s) 284, 316, 1507, 2629, 3318, 3365
Bso31I GGTCTC 1 cut(s) 448
Bsp119I TTCGAA 2 cut(s) 1147, 2518
Bsp1286I GDGCHC 7 cut(s) 208, 313, 700, 1531, 2269, 2898, 2989
Bsp13I TCCGGA 2 cut(s) 1330, 2284
Bsp19I CCATGG 4 cut(s) 279, 517, 1520, 2763
BspANI GGCC 6 cut(s) 284, 316, 1507, 2629, 3318, 3365
BspCNI CTCAG 7 cut(s) 135, 643, 667, 1336, 1435, 3052, 3187
BspDI ATCGAT 2 cut(s) 1635, 3358
BspEI TCCGGA 2 cut(s) 1330, 2284
BspFNI CGCG 2 cut(s) 768, 1808
BspHI TCATGA 2 cut(s) 1824, 2419
BspMI ACCTGC 3 cut(s) 466, 2117, 3517
BspPI GGATC 9 cut(s) 418, 863, 1640, 1708, 1721, 1827, 2042, 2055, 3428
BspQI GCTCTTC 1 cut(s) 2994
BspT104I TTCGAA 2 cut(s) 1147, 2518
BspT107I GGYRCC 5 cut(s) 695, 1426, 1559, 1661, 3005
BspTNI GGTCTC 1 cut(s) 448
BsrBI CCGCTC 1 cut(s) 3173
BsrFI RCCGGY 1 cut(s) 3499
BsrI ACTGG 4 cut(s) 336, 469, 2738, 2858
BssAI RCCGGY 1 cut(s) 3499
BssNI GRCGYC 3 cut(s) 445, 754, 1427
BssSI CACGAG 1 cut(s) 318
BssT1I CCWWGG 6 cut(s) 279, 517, 1177, 1520, 2763, 3260
Bst2BI CACGAG 1 cut(s) 318
Bst2UI CCWGG 5 cut(s) 125, 184, 701, 942, 1356
Bst4CI ACNGT 3 cut(s) 1606, 2095, 2810
BstACI GRCGYC 3 cut(s) 445, 754, 1427
BstAPI GCANNNNNTGC 2 cut(s) 2573, 3619
BstBI TTCGAA 2 cut(s) 1147, 2518
BstC8I GCNNGC 5 cut(s) 780, 852, 1914, 2228, 2433
BstDSI CCRYGG 5 cut(s) 279, 517, 1520, 1806, 2763
BstENI CCTNNNNNAGG 1 cut(s) 3264
BstFNI CGCG 2 cut(s) 768, 1808
BstH2I RGCGCY 2 cut(s) 1430, 2143
BstHHI GCGC 3 cut(s) 770, 1429, 2142
BstMAI GTCTC 6 cut(s) 448, 939, 963, 1521, 1561, 2458
BstMCI CGRYCG 1 cut(s) 3517
BstNI CCWGG 5 cut(s) 125, 184, 701, 942, 1356
BstNSI RCATGY 4 cut(s) 782, 956, 1516, 1916
BstSCI CCNGG 9 cut(s) 123, 182, 699, 871, 940, 1354, 1402, 2581, 3383
BstSFI CTRYAG 2 cut(s) 1314, 1389
BstSLI GKGCMC 1 cut(s) 700
BstUI CGCG 2 cut(s) 768, 1808
BstV2I GAAGAC 4 cut(s) 663, 1472, 2089, 2507
BstX2I RGATCY 4 cut(s) 1713, 1819, 2047, 3214
BstXI CCANNNNNNTGG 1 cut(s) 948
BstYI RGATCY 4 cut(s) 1713, 1819, 2047, 3214
Bsu15I ATCGAT 2 cut(s) 1635, 3358
Bsu36I CCTNAGG 1 cut(s) 1344
BsuI GTATCC 4 cut(s) 1270, 3381, 3418, 3639
BsuRI GGCC 6 cut(s) 284, 316, 1507, 2629, 3318, 3365
BsuTUI ATCGAT 2 cut(s) 1635, 3358
BtgI CCRYGG 5 cut(s) 279, 517, 1520, 1806, 2763
BtrI CACGTC 1 cut(s) 2330
BtsI GCAGTG 3 cut(s) 712, 2073, 3024
BtsIMutI CAGTG 8 cut(s) 712, 1611, 2073, 2091, 2228, 2745, 2865, 3024
BveI ACCTGC 3 cut(s) 466, 2117, 3517
Cac8I GCNNGC 5 cut(s) 780, 852, 1914, 2228, 2433
CciI TCATGA 2 cut(s) 1824, 2419
CfoI GCGC 3 cut(s) 770, 1429, 2142
Cfr10I RCCGGY 1 cut(s) 3499
Cfr13I GGNCC 8 cut(s) 180, 332, 344, 598, 636, 2752, 2956, 3317
Cfr42I CCGCGG 1 cut(s) 1809
ClaI ATCGAT 2 cut(s) 1635, 3358
CseI GACGC 1 cut(s) 272
Csp6I GTAC 4 cut(s) 804, 828, 2790, 3006
CspAI ACCGGT 1 cut(s) 3499
CviQI GTAC 4 cut(s) 804, 828, 2790, 3006
DinI GGCGCC 1 cut(s) 1428
EaeI YGGCCR 3 cut(s) 282, 314, 2627
EciI GGCGGA 1 cut(s) 2906
Ecl136II GAGCTC 2 cut(s) 2896, 2987
Eco130I CCWWGG 6 cut(s) 279, 517, 1177, 1520, 2763, 3260
Eco24I GRGCYC 3 cut(s) 208, 2898, 2989
Eco31I GGTCTC 1 cut(s) 448
Eco32I GATATC 1 cut(s) 1882
Eco47I GGWCC 7 cut(s) 180, 332, 344, 598, 636, 2752, 2956
Eco53kI GAGCTC 2 cut(s) 2896, 2987
Eco57I CTGAAG 6 cut(s) 360, 589, 657, 2694, 2975, 3004
Eco81I CCTNAGG 1 cut(s) 1344
EcoICRI GAGCTC 2 cut(s) 2896, 2987
EcoNI CCTNNNNNAGG 1 cut(s) 3264
EcoO109I RGGNCCY 1 cut(s) 2956
EcoRI GAATTC 1 cut(s) 66
EcoRII CCWGG 5 cut(s) 123, 182, 699, 940, 1354
EcoRV GATATC 1 cut(s) 1882
EcoT14I CCWWGG 6 cut(s) 279, 517, 1177, 1520, 2763, 3260
EcoT22I ATGCAT 1 cut(s) 1577
EcoT38I GRGCYC 3 cut(s) 208, 2898, 2989
EgeI GGCGCC 1 cut(s) 1428
EheI GGCGCC 1 cut(s) 1428
ErhI CCWWGG 6 cut(s) 279, 517, 1177, 1520, 2763, 3260
Esp3I CGTCTC 1 cut(s) 939
FalI AAGNNNNNCTT 4 cut(s) 11, 43, 2240, 2272
FaqI GGGAC 5 cut(s) 318, 741, 1739, 2765, 2969
FauI CCCGC 3 cut(s) 726, 1813, 3558
FauNDI CATATG 2 cut(s) 550, 2569
FbaI TGATCA 1 cut(s) 1648
FriOI GRGCYC 3 cut(s) 208, 2898, 2989
FspBI CTAG 8 cut(s) 462, 687, 771, 897, 1509, 2325, 2336, 2382
GlaI GCGC 3 cut(s) 769, 1428, 2141
GsaI CCCAGC 2 cut(s) 733, 793
GsuI CTGGAG 5 cut(s) 699, 795, 1428, 2447, 3357
HaeII RGCGCY 2 cut(s) 1430, 2143
HaeIII GGCC 6 cut(s) 284, 316, 1507, 2629, 3318, 3365
HapII CCGG 9 cut(s) 531, 873, 1331, 1404, 1532, 2285, 2582, 3385, 3500
HgaI GACGC 1 cut(s) 272
HhaI GCGC 3 cut(s) 770, 1429, 2142
Hin1I GRCGYC 3 cut(s) 445, 754, 1427
Hin6I GCGC 3 cut(s) 768, 1427, 2140
HinP1I GCGC 3 cut(s) 768, 1427, 2140
HincII GTYRAC 1 cut(s) 2187
HindII GTYRAC 1 cut(s) 2187
HindIII AAGCTT 1 cut(s) 3296
HpaII CCGG 9 cut(s) 531, 873, 1331, 1404, 1532, 2285, 2582, 3385, 3500
HphI GGTGA 7 cut(s) 983, 988, 1789, 2752, 2827, 2830, 3245
Hpy166II GTNNAC 4 cut(s) 752, 1891, 2187, 3347
Hpy8I GTNNAC 4 cut(s) 752, 1891, 2187, 3347
HpyAV CCTTC 8 cut(s) 88, 101, 1016, 1900, 2027, 2605, 2718, 3447
HpyCH4III ACNGT 3 cut(s) 1606, 2095, 2810
HpyCH4IV ACGT 4 cut(s) 445, 754, 830, 2329
HpySE526I ACGT 4 cut(s) 445, 754, 830, 2329
Hsp92I GRCGYC 3 cut(s) 445, 754, 1427
HspAI GCGC 3 cut(s) 768, 1427, 2140
KasI GGCGCC 1 cut(s) 1426
KflI GGGWCCC 1 cut(s) 2956
Kpn2I TCCGGA 2 cut(s) 1330, 2284
KpnI GGTACC 1 cut(s) 3009
Ksp22I TGATCA 1 cut(s) 1648
KspI CCGCGG 1 cut(s) 1809
LguI GCTCTTC 1 cut(s) 2994
LweI GCATC 5 cut(s) 1770, 1930, 2308, 2340, 3609
MaeI CTAG 8 cut(s) 462, 687, 771, 897, 1509, 2325, 2336, 2382
MaeII ACGT 4 cut(s) 445, 754, 830, 2329
MaeIII GTNAC 5 cut(s) 446, 1600, 1749, 2095, 3127
MbiI CCGCTC 1 cut(s) 3173
MfeI CAATTG 2 cut(s) 1472, 2387
MflI RGATCY 4 cut(s) 1713, 1819, 2047, 3214
MhlI GDGCHC 7 cut(s) 208, 313, 700, 1531, 2269, 2898, 2989
MlsI TGGCCA 2 cut(s) 284, 2629
MluNI TGGCCA 2 cut(s) 284, 2629
Mly113I GGCGCC 1 cut(s) 1427
MlyI GAGTC 7 cut(s) 16, 299, 757, 1462, 2521, 2826, 3156
Mox20I TGGCCA 2 cut(s) 284, 2629
Mph1103I ATGCAT 1 cut(s) 1577
MroI TCCGGA 2 cut(s) 1330, 2284
MroXI GAANNNNTTC 3 cut(s) 1308, 1398, 3501
MscI TGGCCA 2 cut(s) 284, 2629
MseI TTAA 9 cut(s) 18, 863, 1029, 1046, 1238, 1545, 2202, 3119, 3144
MslI CAYNNNNRTG 5 cut(s) 918, 2093, 2594, 2738, 2762
Msp20I TGGCCA 2 cut(s) 284, 2629
MspA1I CMGCKG 7 cut(s) 389, 677, 733, 1217, 1448, 1808, 2030
MspI CCGG 9 cut(s) 531, 873, 1331, 1404, 1532, 2285, 2582, 3385, 3500
MspR9I CCNGG 9 cut(s) 125, 184, 701, 873, 942, 1356, 1404, 2583, 3385
MunI CAATTG 2 cut(s) 1472, 2387
MvaI CCWGG 5 cut(s) 125, 184, 701, 942, 1356
MvnI CGCG 2 cut(s) 768, 1808
NarI GGCGCC 1 cut(s) 1427
NciI CCSGG 4 cut(s) 873, 1404, 2583, 3385
NcoI CCATGG 4 cut(s) 279, 517, 1520, 2763
NdeI CATATG 2 cut(s) 550, 2569
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 4 cut(s) 446, 1600, 1749, 2095
NsiI ATGCAT 1 cut(s) 1577
NspI RCATGY 4 cut(s) 782, 956, 1516, 1916
NspV TTCGAA 2 cut(s) 1147, 2518
OliI CACNNNNGTG 1 cut(s) 2093
PaeI GCATGC 2 cut(s) 782, 1916
PagI TCATGA 2 cut(s) 1824, 2419
PaqCI CACCTGC 1 cut(s) 3517
PasI CCCWGGG 1 cut(s) 700
PciSI GCTCTTC 1 cut(s) 2994
PcsI WCGNNNNNNNCGW 1 cut(s) 2959
PdmI GAANNNNTTC 3 cut(s) 1308, 1398, 3501
PfeI GAWTC 6 cut(s) 132, 1594, 2104, 2936, 3508, 3602
PflFI GACNNNGTC 1 cut(s) 2331
PfoI TCCNGGA 4 cut(s) 123, 871, 1402, 3383
PinAI ACCGGT 1 cut(s) 3499
PleI GAGTC 7 cut(s) 16, 298, 757, 1462, 2520, 2826, 3156
PluTI GGCGCC 1 cut(s) 1430
PpsI GAGTC 7 cut(s) 16, 298, 757, 1462, 2520, 2826, 3156
PpuMI RGGWCCY 1 cut(s) 2956
Psp124BI GAGCTC 2 cut(s) 2898, 2989
Psp5II RGGWCCY 1 cut(s) 2956
Psp6I CCWGG 5 cut(s) 123, 182, 699, 940, 1354
PspFI CCCAGC 2 cut(s) 729, 789
PspGI CCWGG 5 cut(s) 123, 182, 699, 940, 1354
PspPI GGNCC 8 cut(s) 180, 332, 344, 598, 636, 2752, 2956, 3317
PspPPI RGGWCCY 1 cut(s) 2956
PsuI RGATCY 4 cut(s) 1713, 1819, 2047, 3214
PsyI GACNNNGTC 1 cut(s) 2331
PvuII CAGCTG 2 cut(s) 389, 677
RsaI GTAC 4 cut(s) 805, 829, 2791, 3007
RsaNI GTAC 4 cut(s) 804, 828, 2790, 3006
RseI CAYNNNNRTG 5 cut(s) 918, 2093, 2594, 2738, 2762
SacI GAGCTC 2 cut(s) 2898, 2989
SacII CCGCGG 1 cut(s) 1809
SapI GCTCTTC 1 cut(s) 2994
SaqAI TTAA 9 cut(s) 18, 863, 1029, 1046, 1238, 1545, 2202, 3119, 3144
Sau96I GGNCC 8 cut(s) 180, 332, 344, 598, 636, 2752, 2956, 3317
SchI GAGTC 7 cut(s) 16, 299, 757, 1462, 2521, 2826, 3156
ScrFI CCNGG 9 cut(s) 125, 184, 701, 873, 942, 1356, 1404, 2583, 3385
SduI GDGCHC 7 cut(s) 208, 313, 700, 1531, 2269, 2898, 2989
SfaNI GCATC 5 cut(s) 1770, 1930, 2308, 2340, 3609
SfcI CTRYAG 2 cut(s) 1314, 1389
SfoI GGCGCC 1 cut(s) 1428
Sfr303I CCGCGG 1 cut(s) 1809
SfuI TTCGAA 2 cut(s) 1147, 2518
SgrBI CCGCGG 1 cut(s) 1809
SinI GGWCC 7 cut(s) 180, 332, 344, 598, 636, 2752, 2956
SmiMI CAYNNNNRTG 5 cut(s) 918, 2093, 2594, 2738, 2762
SmlI CTYRAG 4 cut(s) 1894, 2453, 2720, 3164
SmoI CTYRAG 4 cut(s) 1894, 2453, 2720, 3164
SphI GCATGC 2 cut(s) 782, 1916
SspDI GGCGCC 1 cut(s) 1426
SspI AATATT 1 cut(s) 1935
SspMI CTAG 8 cut(s) 462, 687, 771, 897, 1509, 2325, 2336, 2382
SstI GAGCTC 2 cut(s) 2898, 2989
StyD4I CCNGG 9 cut(s) 123, 182, 699, 871, 940, 1354, 1402, 2581, 3383
StyI CCWWGG 6 cut(s) 279, 517, 1177, 1520, 2763, 3260
TaaI ACNGT 3 cut(s) 1606, 2095, 2810
TaiI ACGT 4 cut(s) 448, 757, 833, 2332
TaqII GACCGA 3 cut(s) 703, 1737, 3531
TauI GCSGC 2 cut(s) 1811, 2398
TfiI GAWTC 6 cut(s) 132, 1594, 2104, 2936, 3508, 3602
Tru1I TTAA 9 cut(s) 18, 863, 1029, 1046, 1238, 1545, 2202, 3119, 3144
Tru9I TTAA 9 cut(s) 18, 863, 1029, 1046, 1238, 1545, 2202, 3119, 3144
TscAI CASTG 8 cut(s) 712, 1611, 2073, 2098, 2228, 2745, 2865, 3031
TseFI GTSAC 4 cut(s) 446, 1600, 1749, 2095
Tsp45I GTSAC 4 cut(s) 446, 1600, 1749, 2095
TspGWI ACGGA 3 cut(s) 1611, 2114, 3194
TspRI CASTG 8 cut(s) 712, 1611, 2073, 2098, 2228, 2745, 2865, 3031
Tth111I GACNNNGTC 1 cut(s) 2331
VpaK11BI GGWCC 7 cut(s) 180, 332, 344, 598, 636, 2752, 2956
XagI CCTNNNNNAGG 1 cut(s) 3264
XapI RAATTY 4 cut(s) 66, 1769, 2975, 3221
XbaI TCTAGA 1 cut(s) 2324
XceI RCATGY 4 cut(s) 782, 956, 1516, 1916
XcmI CCANNNNNNNNNTGG 3 cut(s) 292, 737, 2745
XmnI GAANNNNTTC 3 cut(s) 1308, 1398, 3501
XspI CTAG 8 cut(s) 462, 687, 771, 897, 1509, 2325, 2336, 2382
ZraI GACGTC 2 cut(s) 446, 755
Zsp2I ATGCAT 1 cut(s) 1577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.