pycom12g20730

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000094
Physical Location & Seq
Forward (+)
22116505 .. 22117332
828 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g20730.2

Sequence Viewer

Length: 720 bp
ATGCCTTTCGGATTGACGAATGCTCCTGCAGCGTTCATGGATTTGATGAACAGAGTCTTCAGACCATATCTGGACCGGTTTGTGATTGTGTTCATCGATGACATTCTGATTTATTCCAAAAGTGTTGACGAACACATGAAACATTTAAGGTTGGTGCTGGAAAGGTTGAGAAGTCGGCAGCTGTATGCTAAGTTCACCAAGTGTCAGTTTTGGCTTAACCAAATCAGCTTTCTCGGACATGTGGTATCTGCTGAAGGAATTAGTGTGGATCCTCAGAAGGTGTCGGCAATAACTACATGGGAGCAACCGAGGACTGTTACAGAGGTGAGGAGTTTCCTAGGTCTGGCTAGCTATTATCGACGTTTTGTGCAGGGTTTCTCAGCAATTGCCTTGCCTTTGAGTAAGTTGACTCGGAAGGAGGTTGAGTTTAAGTGGGATGAAGATTGTGAGCGGAGTTTTCAGGAGCTGAAACGACGTCTCACTCAAGCTCCCGTTCTTGTCCTTCCAGACGATAGCGGCGAGTTTGAAATCTACACGGATGCATTTGGTTCAGTTGTGTTCGCTCTGAAGATCTGGAGACACTATTTGTATGGTGAGAAGTGCCGCATCTTCACCGATCATAAGAGCCTCAAGTATATCTTTACGCAGAAAGACTTGAATTTGAGGCAGAGAAGGTGGATGGAGCTCATCAGTGAGTATAACTGCACGATCGATCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve zf-CCHC

Protein Analysis

240

Amino Acids

28.32

Weight (kDa)

8.97

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000094)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42190
fragaria_vesca FvH4_1g22371 FvH4_1g23261 FvH4_1g27933 FvH4_1g27934 FvH4_1g29231 FvH4_2g02781 FvH4_2g08031 FvH4_2g08581 FvH4_3g06961 FvH4_3g06962 FvH4_3g13531 FvH4_3g14391 FvH4_3g14392 FvH4_3g18622 FvH4_3g18964 FvH4_3g26314 FvH4_3g28781 FvH4_3g30771 FvH4_3g30772 FvH4_3g31752 FvH4_3g32803 FvH4_3g35751 FvH4_3g35752 FvH4_4g02151 FvH4_4g02642 FvH4_4g05523 FvH4_4g05524 FvH4_4g06574 FvH4_4g06722 FvH4_4g08644 FvH4_4g08645 FvH4_4g08646 FvH4_4g08851 FvH4_4g08971 FvH4_4g09081 FvH4_4g09243 FvH4_4g09244 FvH4_4g09283 FvH4_4g10021 FvH4_4g10032 FvH4_4g10033 FvH4_4g10182 FvH4_4g10361 FvH4_4g12502 FvH4_4g12503 FvH4_4g13341 FvH4_4g13942 FvH4_4g13943 FvH4_4g13944 FvH4_4g14432 FvH4_4g14822 FvH4_4g14823 FvH4_4g15291 FvH4_4g15292 FvH4_4g15293 FvH4_4g15471 FvH4_4g15583 FvH4_4g15584 FvH4_4g15585 FvH4_4g15586 FvH4_4g15587 FvH4_4g15591 FvH4_4g15592 FvH4_4g15593 FvH4_4g16179 FvH4_4g18131 FvH4_4g21031 FvH4_4g22021 FvH4_4g22021 FvH4_4g22042 FvH4_5g03402 FvH4_5g07411 FvH4_5g13881 FvH4_5g15792 FvH4_5g24682 FvH4_5g24911 FvH4_5g25322 FvH4_5g25641 FvH4_5g36692 FvH4_5g38072 FvH4_5g38171 FvH4_6g05202 FvH4_6g08531 FvH4_6g16342 FvH4_6g21771 FvH4_6g23303 FvH4_6g23961 FvH4_6g25381 FvH4_6g27021 FvH4_6g27022 FvH4_6g30091 FvH4_6g30092 FvH4_7g02051 FvH4_7g06031 FvH4_7g07031 FvH4_7g07581 FvH4_7g08381 FvH4_7g11551 FvH4_7g19711 FvH4_7g25621 FvH4_7g29722
malus_domestica MD06G1134500.v1.1 MD09G1067600.v1.1 MD13G1145500.v1.1 MD15G1166500.v1.1 MD16G1270000.v1.1
prunus_persica Prupe.7G030900_v2.0.a1
pyrus_communis pycom01g00710 pycom01g03760 pycom03g10770 pycom04g02180 pycom05g08050 pycom06g04140 pycom06g11110 pycom07g07550 pycom07g21570 pycom08g17240 pycom09g13900 pycom09g19570 pycom10g22300 pycom11g03210 pycom11g13750 pycom11g24890 pycom12561g00270 pycom12668g00020 pycom12g11280 pycom12g20720 pycom12g20730 pycom13g21410 pycom13g21660 pycom14g06010 pycom14g06370 pycom14g12800 pycom16g21440 pycom16g22530 pycom17g02030 pycom420g00170 pycom50g00020 pycom5153684g00110 pycom520g00860 pycom581g00060
rosa_chinensis RchiOBHm_Chr0c08g0498091 RchiOBHm_Chr0c08g0498101 RchiOBHm_Chr1g0326991 RchiOBHm_Chr2g0084311 RchiOBHm_Chr2g0090701 RchiOBHm_Chr2g0098591 RchiOBHm_Chr2g0109491 RchiOBHm_Chr2g0109501 RchiOBHm_Chr2g0130801 RchiOBHm_Chr2g0145931 RchiOBHm_Chr2g0168091 RchiOBHm_Chr3g0457081 RchiOBHm_Chr3g0457091 RchiOBHm_Chr3g0467781 RchiOBHm_Chr3g0467791 RchiOBHm_Chr3g0493771 RchiOBHm_Chr3g0494051 RchiOBHm_Chr3g0496881 RchiOBHm_Chr4g0401391 RchiOBHm_Chr4g0414861 RchiOBHm_Chr4g0424081 RchiOBHm_Chr5g0009611 RchiOBHm_Chr5g0049881 RchiOBHm_Chr5g0050351 RchiOBHm_Chr5g0053791 RchiOBHm_Chr5g0078031 RchiOBHm_Chr6g0256481 RchiOBHm_Chr6g0271231 RchiOBHm_Chr6g0286381 RchiOBHm_Chr7g0197101 RchiOBHm_Chr7g0221891 RchiOBHm_Chr7g0237801 RchiOBHm_Chr7g0237821
rosa_multiflora Rmu_sc0000509.1_g000034 Rmu_sc0004751.1_g000014 Rmu_sc0008546.1_g000002 Rmu_sc0009320.1_g000003 Rmu_sc0010661.1_g000006 Rmu_sc0029652.1_g000002
rosa_roxburghii Rroxscaffold_10G00433150 Rroxscaffold_15G00447630 Rroxscaffold_26G00447790 Rroxscaffold_29G00441810 Rroxscaffold_32G00442860 Rroxscaffold_33G00439800 Rroxscaffold_34G00443230 Rroxscaffold_3G00220560 Rroxscaffold_3G00227370 Rroxscaffold_3G00239080 Rroxscaffold_3G00239090 Rroxscaffold_3G00273190 Rroxscaffold_4G00323530 Rroxscaffold_4G00326840 Rroxscaffold_57G00443110 Rroxscaffold_5G00338590 Rroxscaffold_5G00347530 Rroxscaffold_68G00446990 Rroxscaffold_76G00448570 Rroxscaffold_90G00448860
rosa_rugosa Rorug06G0233600
rosa_samantha Rh3CG302700 Rh6DG286700
rosa_wichuraiana Rw0G002280 Rw0G004080 Rw0G011830 Rw1G003690 Rw1G008800 Rw1G009130 Rw1G009140 Rw1G009150 Rw1G009160 Rw1G009410 Rw1G009580 Rw1G012770 Rw1G015850 Rw1G026860 Rw2G003780 Rw2G006480 Rw2G016520 Rw2G016960 Rw2G018170 Rw2G025180 Rw2G025510 Rw2G025610 Rw2G025750 Rw2G025920 Rw2G029950 Rw2G046360 Rw2G046370 Rw2G046380 Rw3G009070 Rw3G025820 Rw3G027340 Rw3G027900 Rw3G028150 Rw3G029060 Rw4G002440 Rw4G005960 Rw4G007520 Rw4G011970 Rw4G011980 Rw4G012190 Rw4G012530 Rw4G018490 Rw4G026380 Rw5G003970 Rw5G030920 Rw5G031780 Rw5G031900 Rw5G033030 Rw5G033040 Rw5G036330 Rw5G039730 Rw5G048110 Rw6G006670 Rw6G007910 Rw6G009720 Rw6G010990 Rw6G012930 Rw6G012940 Rw6G013420 Rw6G014690 Rw6G015290 Rw6G015790 Rw6G015920 Rw6G036510 Rw7G016650 Rw7G023140 Rw7G028650 Rw7G036450 Rw7G036460 Rw7G036910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 478
AccBSI CCGCTC 1 cut(s) 451
AciI CCGC 3 cut(s) 451, 516, 604
AclWI GGATC 2 cut(s) 263, 276
AcsI RAATTY 1 cut(s) 658
AcuI CTGAAG 3 cut(s) 43, 273, 587
AcyI GRCGYC 1 cut(s) 475
AdeI CACNNNGTG 1 cut(s) 201
AfiI CCNNNNNNNGG 1 cut(s) 343
AflIII ACRYGT 1 cut(s) 238
AgeI ACCGGT 1 cut(s) 75
AgsI TTSAA 2 cut(s) 527, 658
AjuI GAANNNNNNNTTGG 2 cut(s) 213, 245
AluBI AGCT 6 cut(s) 181, 228, 351, 466, 488, 685
AluI AGCT 6 cut(s) 181, 228, 351, 466, 488, 685
Alw21I GWGCWC 1 cut(s) 687
Alw26I GTCTC 2 cut(s) 482, 571
AlwI GGATC 2 cut(s) 263, 276
AlwNI CAGNNNCTG 1 cut(s) 466
ApeKI GCWGC 2 cut(s) 29, 178
ApoI RAATTY 1 cut(s) 658
AsiGI ACCGGT 1 cut(s) 75
AspA2I CCTAGG 1 cut(s) 337
AspS9I GGNCC 1 cut(s) 73
AsuHPI GGTGA 4 cut(s) 187, 337, 604, 605
AsuNHI GCTAGC 1 cut(s) 347
AvaII GGWCC 1 cut(s) 73
AvrII CCTAGG 1 cut(s) 337
BamHI GGATCC 1 cut(s) 268
BanII GRGCYC 1 cut(s) 687
BbsI GAAGAC 1 cut(s) 49
Bbv12I GWGCWC 1 cut(s) 687
BbvI GCAGC 2 cut(s) 41, 190
BccI CCATC 1 cut(s) 673
BcoDI GTCTC 2 cut(s) 482, 571
BfaI CTAG 2 cut(s) 338, 348
BfmI CTRYAG 1 cut(s) 27
BglII AGATCT 1 cut(s) 570
BisI GCNGC 4 cut(s) 30, 179, 517, 604
BlnI CCTAGG 1 cut(s) 337
BlsI GCNGC 4 cut(s) 31, 180, 518, 605
Bme18I GGWCC 1 cut(s) 73
BmgT120I GGNCC 1 cut(s) 73
BmiI GGNNCC 1 cut(s) 270
BmsI GCATC 2 cut(s) 529, 615
BmtI GCTAGC 1 cut(s) 351
BpiI GAAGAC 1 cut(s) 49
BpmI CTGGAG 1 cut(s) 595
BpuEI CTTGAG 2 cut(s) 468, 614
Bsa29I ATCGAT 2 cut(s) 96, 711
BsaHI GRCGYC 1 cut(s) 475
BsaJI CCNNGG 2 cut(s) 308, 337
BsaWI WCCGGW 1 cut(s) 75
BsaXI ACNNNNNCTCC 6 cut(s) 7, 37, 410, 440, 472, 502
Bsc4I CCNNNNNNNGG 1 cut(s) 343
Bse118I RCCGGY 1 cut(s) 75
BseCI ATCGAT 2 cut(s) 96, 711
BseDI CCNNGG 2 cut(s) 308, 337
BseGI GGATG 3 cut(s) 442, 544, 684
BseLI CCNNNNNNNGG 1 cut(s) 343
BseMII CTCAG 2 cut(s) 287, 393
BseRI GAGGAG 1 cut(s) 343
BseXI GCAGC 2 cut(s) 41, 190
BsgI GTGCAG 2 cut(s) 389, 688
Bsh1285I CGRYCG 1 cut(s) 711
BshTI ACCGGT 1 cut(s) 75
BshVI ATCGAT 2 cut(s) 96, 711
BsiEI CGRYCG 1 cut(s) 711
BsiHKAI GWGCWC 1 cut(s) 687
BsiSI CCGG 1 cut(s) 76
BslI CCNNNNNNNGG 1 cut(s) 343
BsmAI GTCTC 2 cut(s) 482, 571
BsmBI CGTCTC 1 cut(s) 482
BsmI GAATGC 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 687
Bsp143I GATC 5 cut(s) 268, 570, 616, 708, 712
BspACI CCGC 3 cut(s) 451, 516, 604
BspCNI CTCAG 2 cut(s) 286, 392
BspDI ATCGAT 2 cut(s) 96, 711
BspLI GGNNCC 1 cut(s) 270
BspMAI CTGCAG 1 cut(s) 31
BspOI GCTAGC 1 cut(s) 351
BspPI GGATC 2 cut(s) 263, 276
BsrBI CCGCTC 1 cut(s) 451
BsrFI RCCGGY 1 cut(s) 75
BssAI RCCGGY 1 cut(s) 75
BssECI CCNNGG 2 cut(s) 308, 337
BssMI GATC 5 cut(s) 268, 570, 616, 708, 712
BssNI GRCGYC 1 cut(s) 475
BssT1I CCWWGG 1 cut(s) 337
Bst4CI ACNGT 1 cut(s) 316
BstACI GRCGYC 1 cut(s) 475
BstC8I GCNNGC 1 cut(s) 349
BstDEI CTNAG 3 cut(s) 189, 273, 379
BstF5I GGATG 3 cut(s) 442, 544, 684
BstKTI GATC 5 cut(s) 271, 573, 619, 711, 715
BstMAI GTCTC 2 cut(s) 482, 571
BstMBI GATC 5 cut(s) 268, 570, 616, 708, 712
BstMCI CGRYCG 1 cut(s) 711
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNSI RCATGY 1 cut(s) 242
BstSFI CTRYAG 1 cut(s) 27
BstV1I GCAGC 2 cut(s) 41, 190
BstV2I GAAGAC 1 cut(s) 49
BstX2I RGATCY 2 cut(s) 268, 570
BstYI RGATCY 2 cut(s) 268, 570
Bsu15I ATCGAT 2 cut(s) 96, 711
BsuTUI ATCGAT 2 cut(s) 96, 711
BtsCI GGATG 3 cut(s) 442, 544, 684
BtsIMutI CAGTG 1 cut(s) 697
Cac8I GCNNGC 1 cut(s) 349
CaiI CAGNNNCTG 1 cut(s) 466
Cfr10I RCCGGY 1 cut(s) 75
Cfr13I GGNCC 1 cut(s) 73
ClaI ATCGAT 2 cut(s) 96, 711
CspAI ACCGGT 1 cut(s) 75
CviAII CATG 4 cut(s) 37, 136, 239, 297
CviJI RGCY 9 cut(s) 181, 214, 228, 347, 351, 466, 488, 627, 685
CviKI_1 RGCY 9 cut(s) 181, 214, 228, 347, 351, 466, 488, 627, 685
DdeI CTNAG 3 cut(s) 189, 273, 379
DpnI GATC 5 cut(s) 270, 572, 618, 710, 714
DpnII GATC 5 cut(s) 268, 570, 616, 708, 712
DraIII CACNNNGTG 1 cut(s) 201
Ecl136II GAGCTC 1 cut(s) 685
Eco130I CCWWGG 1 cut(s) 337
Eco24I GRGCYC 1 cut(s) 687
Eco47I GGWCC 1 cut(s) 73
Eco53kI GAGCTC 1 cut(s) 685
Eco57I CTGAAG 3 cut(s) 43, 273, 587
EcoICRI GAGCTC 1 cut(s) 685
EcoT14I CCWWGG 1 cut(s) 337
EcoT22I ATGCAT 1 cut(s) 544
EcoT38I GRGCYC 1 cut(s) 687
ErhI CCWWGG 1 cut(s) 337
Esp3I CGTCTC 1 cut(s) 482
FaeI CATG 4 cut(s) 40, 139, 242, 300
FalI AAGNNNNNCTT 2 cut(s) 623, 655
FatI CATG 4 cut(s) 36, 135, 238, 296
Fnu4HI GCNGC 4 cut(s) 30, 179, 517, 604
FokI GGATG 3 cut(s) 449, 551, 691
FriOI GRGCYC 1 cut(s) 687
Fsp4HI GCNGC 4 cut(s) 30, 179, 517, 604
FspBI CTAG 2 cut(s) 338, 348
GluI GCNGC 4 cut(s) 30, 179, 517, 604
GsuI CTGGAG 1 cut(s) 595
HapII CCGG 1 cut(s) 76
Hin1I GRCGYC 1 cut(s) 475
Hin1II CATG 4 cut(s) 40, 139, 242, 300
HincII GTYRAC 2 cut(s) 127, 408
HindII GTYRAC 2 cut(s) 127, 408
HinfI GANTC 2 cut(s) 54, 409
HpaII CCGG 1 cut(s) 76
HphI GGTGA 4 cut(s) 187, 337, 604, 605
Hpy166II GTNNAC 3 cut(s) 127, 195, 408
Hpy188I TCNGA 7 cut(s) 11, 62, 108, 236, 276, 414, 567
Hpy188III TCNNGA 4 cut(s) 71, 461, 506, 574
Hpy8I GTNNAC 3 cut(s) 127, 195, 408
Hpy99I CGWCG 2 cut(s) 363, 477
HpyAV CCTTC 5 cut(s) 248, 271, 409, 512, 666
HpyCH4III ACNGT 1 cut(s) 316
HpyCH4IV ACGT 2 cut(s) 361, 475
HpyCH4V TGCA 4 cut(s) 29, 370, 542, 705
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 3 cut(s) 189, 273, 379
HpySE526I ACGT 2 cut(s) 361, 475
Hsp92I GRCGYC 1 cut(s) 475
Hsp92II CATG 4 cut(s) 40, 139, 242, 300
Kzo9I GATC 5 cut(s) 268, 570, 616, 708, 712
LmnI GCTCC 5 cut(s) 28, 301, 463, 493, 682
LpnPI CCDG 9 cut(s) 39, 56, 89, 143, 329, 356, 446, 519, 559
Lsp1109I GCAGC 2 cut(s) 41, 190
LweI GCATC 2 cut(s) 529, 615
MaeI CTAG 2 cut(s) 338, 348
MaeII ACGT 2 cut(s) 361, 475
MaeIII GTNAC 1 cut(s) 316
MalI GATC 5 cut(s) 270, 572, 618, 710, 714
MbiI CCGCTC 1 cut(s) 451
MboI GATC 5 cut(s) 268, 570, 616, 708, 712
MboII GAAGA 4 cut(s) 49, 452, 580, 601
MfeI CAATTG 1 cut(s) 384
MflI RGATCY 2 cut(s) 268, 570
MhlI GDGCHC 1 cut(s) 687
MluCI AATT 3 cut(s) 258, 384, 658
MlyI GAGTC 2 cut(s) 63, 403
MnlI CCTC 7 cut(s) 282, 303, 316, 321, 412, 638, 657
Mph1103I ATGCAT 1 cut(s) 544
MseI TTAA 3 cut(s) 146, 216, 429
MspA1I CMGCKG 1 cut(s) 181
MspI CCGG 1 cut(s) 76
MunI CAATTG 1 cut(s) 384
Mva1269I GAATGC 1 cut(s) 25
MwoI GCNNNNNNNGC 1 cut(s) 29
NdeII GATC 5 cut(s) 268, 570, 616, 708, 712
NheI GCTAGC 1 cut(s) 347
NlaIII CATG 4 cut(s) 40, 139, 242, 300
NlaIV GGNNCC 1 cut(s) 270
NsiI ATGCAT 1 cut(s) 544
NspI RCATGY 1 cut(s) 242
PciI ACATGT 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 516
PctI GAATGC 1 cut(s) 25
PinAI ACCGGT 1 cut(s) 75
PkrI GCNGC 4 cut(s) 31, 180, 518, 605
Ple19I CGATCG 1 cut(s) 711
PleI GAGTC 2 cut(s) 62, 403
PpsI GAGTC 2 cut(s) 62, 403
PscI ACATGT 1 cut(s) 238
Psp124BI GAGCTC 1 cut(s) 687
PspN4I GGNNCC 1 cut(s) 270
PspPI GGNCC 1 cut(s) 73
PsrI GAACNNNNNNTAC 2 cut(s) 176, 208
PstI CTGCAG 1 cut(s) 31
PstNI CAGNNNCTG 1 cut(s) 466
PsuI RGATCY 2 cut(s) 268, 570
PvuI CGATCG 1 cut(s) 711
PvuII CAGCTG 1 cut(s) 181
SacI GAGCTC 1 cut(s) 687
SaqAI TTAA 3 cut(s) 146, 216, 429
SatI GCNGC 4 cut(s) 30, 179, 517, 604
Sau3AI GATC 5 cut(s) 268, 570, 616, 708, 712
Sau96I GGNCC 1 cut(s) 73
SchI GAGTC 2 cut(s) 63, 403
SduI GDGCHC 1 cut(s) 687
SfaNI GCATC 2 cut(s) 529, 615
SfcI CTRYAG 1 cut(s) 27
SinI GGWCC 1 cut(s) 73
SmlI CTYRAG 2 cut(s) 483, 629
SmoI CTYRAG 2 cut(s) 483, 629
Sse9I AATT 3 cut(s) 258, 384, 658
SsiI CCGC 3 cut(s) 451, 516, 604
SspMI CTAG 2 cut(s) 338, 348
SstI GAGCTC 1 cut(s) 687
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 1 cut(s) 316
TaiI ACGT 2 cut(s) 364, 478
TaqI TCGA 3 cut(s) 96, 358, 711
TasI AATT 3 cut(s) 258, 384, 658
TauI GCSGC 2 cut(s) 519, 606
Tru1I TTAA 3 cut(s) 146, 216, 429
Tru9I TTAA 3 cut(s) 146, 216, 429
TscAI CASTG 1 cut(s) 697
TseI GCWGC 2 cut(s) 29, 178
TspDTI ATGAA 5 cut(s) 25, 62, 82, 152, 453
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 1 cut(s) 697
VpaK11BI GGWCC 1 cut(s) 73
XapI RAATTY 1 cut(s) 658
XceI RCATGY 1 cut(s) 242
XmaJI CCTAGG 1 cut(s) 337
XspI CTAG 2 cut(s) 338, 348
ZraI GACGTC 1 cut(s) 476
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.