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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 311 |
| AgsI | TTSAA | 2 cut(s) | 290, 345 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 78, 110 |
| ApoI | RAATTY | 1 cut(s) | 311 |
| AseI | ATTAAT | 1 cut(s) | 66 |
| AsuHPI | GGTGA | 2 cut(s) | 110, 140 |
| BccI | CCATC | 2 cut(s) | 68, 95 |
| BfaI | CTAG | 2 cut(s) | 258, 306 |
| BmsI | GCATC | 1 cut(s) | 268 |
| BseGI | GGATG | 1 cut(s) | 58 |
| Bsp143I | GATC | 1 cut(s) | 361 |
| BssMI | GATC | 1 cut(s) | 361 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstKTI | GATC | 1 cut(s) | 364 |
| BstMBI | GATC | 1 cut(s) | 361 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| CviJI | RGCY | 2 cut(s) | 11, 305 |
| CviKI_1 | RGCY | 2 cut(s) | 11, 305 |
| DpnI | GATC | 1 cut(s) | 363 |
| DpnII | GATC | 1 cut(s) | 361 |
| DraI | TTTAAA | 1 cut(s) | 244 |
| FaiI | YATR | 3 cut(s) | 212, 354, 409 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 78, 110 |
| FokI | GGATG | 1 cut(s) | 65 |
| FspBI | CTAG | 2 cut(s) | 258, 306 |
| HphI | GGTGA | 2 cut(s) | 110, 140 |
| HpyAV | CCTTC | 3 cut(s) | 80, 158, 191 |
| HpyCH4V | TGCA | 1 cut(s) | 281 |
| Kzo9I | GATC | 1 cut(s) | 361 |
| LpnPI | CCDG | 1 cut(s) | 31 |
| LweI | GCATC | 1 cut(s) | 268 |
| MaeI | CTAG | 2 cut(s) | 258, 306 |
| MaeIII | GTNAC | 2 cut(s) | 200, 397 |
| MalI | GATC | 1 cut(s) | 363 |
| MboI | GATC | 1 cut(s) | 361 |
| MboII | GAAGA | 1 cut(s) | 331 |
| MfeI | CAATTG | 1 cut(s) | 345 |
| MluCI | AATT | 3 cut(s) | 63, 311, 345 |
| MnlI | CCTC | 6 cut(s) | 42, 124, 134, 243, 295, 382 |
| MseI | TTAA | 4 cut(s) | 66, 243, 315, 336 |
| MunI | CAATTG | 1 cut(s) | 345 |
| NdeII | GATC | 1 cut(s) | 361 |
| PshBI | ATTAAT | 1 cut(s) | 66 |
| SaqAI | TTAA | 4 cut(s) | 66, 243, 315, 336 |
| Sau3AI | GATC | 1 cut(s) | 361 |
| SetI | ASST | 5 cut(s) | 91, 135, 145, 169, 202 |
| SfaNI | GCATC | 1 cut(s) | 268 |
| Sse9I | AATT | 3 cut(s) | 63, 311, 345 |
| SspMI | CTAG | 2 cut(s) | 258, 306 |
| TasI | AATT | 3 cut(s) | 63, 311, 345 |
| Tru1I | TTAA | 4 cut(s) | 66, 243, 315, 336 |
| Tru9I | TTAA | 4 cut(s) | 66, 243, 315, 336 |
| TspDTI | ATGAA | 2 cut(s) | 177, 198 |
| VspI | ATTAAT | 1 cut(s) | 66 |
| XapI | RAATTY | 1 cut(s) | 311 |
| XspI | CTAG | 2 cut(s) | 258, 306 |