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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 160 |
| AciI | CCGC | 1 cut(s) | 134 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 31, 160 |
| AgsI | TTSAA | 1 cut(s) | 71 |
| AjnI | CCWGG | 1 cut(s) | 24 |
| AoxI | GGCC | 3 cut(s) | 107, 124, 151 |
| AspS9I | GGNCC | 4 cut(s) | 20, 107, 124, 151 |
| AsuHPI | GGTGA | 1 cut(s) | 130 |
| AvaII | GGWCC | 1 cut(s) | 20 |
| BccI | CCATC | 2 cut(s) | 115, 169 |
| BciT130I | CCWGG | 1 cut(s) | 26 |
| Bme1390I | CCNGG | 1 cut(s) | 26 |
| Bme18I | GGWCC | 1 cut(s) | 20 |
| BmgT120I | GGNCC | 4 cut(s) | 20, 107, 124, 151 |
| BmiI | GGNNCC | 1 cut(s) | 22 |
| BmrFI | CCNGG | 1 cut(s) | 26 |
| BsaJI | CCNNGG | 1 cut(s) | 24 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 31, 160 |
| BseBI | CCWGG | 1 cut(s) | 26 |
| BseDI | CCNNGG | 1 cut(s) | 24 |
| BseGI | GGATG | 3 cut(s) | 40, 46, 100 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 31, 160 |
| BshFI | GGCC | 3 cut(s) | 109, 126, 153 |
| BslFI | GGGAC | 1 cut(s) | 6 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 31, 160 |
| BsmFI | GGGAC | 1 cut(s) | 6 |
| BsnI | GGCC | 3 cut(s) | 109, 126, 153 |
| BspACI | CCGC | 1 cut(s) | 134 |
| BspANI | GGCC | 3 cut(s) | 109, 126, 153 |
| BspLI | GGNNCC | 1 cut(s) | 22 |
| BssECI | CCNNGG | 1 cut(s) | 24 |
| Bst2UI | CCWGG | 1 cut(s) | 26 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstF5I | GGATG | 3 cut(s) | 40, 46, 100 |
| BstNI | CCWGG | 1 cut(s) | 26 |
| BstSCI | CCNGG | 1 cut(s) | 24 |
| BsuRI | GGCC | 3 cut(s) | 109, 126, 153 |
| BtsCI | GGATG | 3 cut(s) | 40, 46, 100 |
| Cfr13I | GGNCC | 4 cut(s) | 20, 107, 124, 151 |
| CviAII | CATG | 1 cut(s) | 160 |
| CviJI | RGCY | 4 cut(s) | 109, 126, 153, 180 |
| CviKI_1 | RGCY | 4 cut(s) | 109, 126, 153, 180 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| Eco47I | GGWCC | 1 cut(s) | 20 |
| EcoRII | CCWGG | 1 cut(s) | 24 |
| FaeI | CATG | 1 cut(s) | 163 |
| FaiI | YATR | 2 cut(s) | 105, 161 |
| FaqI | GGGAC | 1 cut(s) | 6 |
| FatI | CATG | 1 cut(s) | 159 |
| FauI | CCCGC | 1 cut(s) | 141 |
| FokI | GGATG | 3 cut(s) | 47, 53, 107 |
| HaeIII | GGCC | 3 cut(s) | 109, 126, 153 |
| Hin1II | CATG | 1 cut(s) | 163 |
| HphI | GGTGA | 1 cut(s) | 130 |
| Hpy188III | TCNNGA | 1 cut(s) | 68 |
| HpyCH4V | TGCA | 1 cut(s) | 4 |
| Hsp92II | CATG | 1 cut(s) | 163 |
| LpnPI | CCDG | 2 cut(s) | 11, 38 |
| MboII | GAAGA | 1 cut(s) | 41 |
| MnlI | CCTC | 4 cut(s) | 25, 31, 37, 82 |
| MslI | CAYNNNNRTG | 1 cut(s) | 164 |
| MspR9I | CCNGG | 1 cut(s) | 26 |
| MvaI | CCWGG | 1 cut(s) | 26 |
| NlaIII | CATG | 1 cut(s) | 163 |
| NlaIV | GGNNCC | 1 cut(s) | 22 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 160 |
| Psp6I | CCWGG | 1 cut(s) | 24 |
| PspGI | CCWGG | 1 cut(s) | 24 |
| PspN4I | GGNNCC | 1 cut(s) | 22 |
| PspPI | GGNCC | 4 cut(s) | 20, 107, 124, 151 |
| RseI | CAYNNNNRTG | 1 cut(s) | 164 |
| Sau96I | GGNCC | 4 cut(s) | 20, 107, 124, 151 |
| ScrFI | CCNGG | 1 cut(s) | 26 |
| SetI | ASST | 2 cut(s) | 9, 93 |
| SgeI | CNNG | 7 cut(s) | 17, 37, 38, 80, 145, 167, 172 |
| SinI | GGWCC | 1 cut(s) | 20 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 164 |
| SsiI | CCGC | 1 cut(s) | 134 |
| StyD4I | CCNGG | 1 cut(s) | 24 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 160 |
| VpaK11BI | GGWCC | 1 cut(s) | 20 |