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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcuI | CTGAAG | 1 cut(s) | 35 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 101 |
| AluBI | AGCT | 2 cut(s) | 13, 69 |
| AluI | AGCT | 2 cut(s) | 13, 69 |
| ApeKI | GCWGC | 1 cut(s) | 10 |
| BbvI | GCAGC | 1 cut(s) | 22 |
| BccI | CCATC | 2 cut(s) | 55, 148 |
| BfaI | CTAG | 1 cut(s) | 181 |
| BisI | GCNGC | 1 cut(s) | 11 |
| BlpI | GCTNAGC | 1 cut(s) | 141 |
| BlsI | GCNGC | 1 cut(s) | 12 |
| BpmI | CTGGAG | 1 cut(s) | 65 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 141 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 101 |
| BseGI | GGATG | 1 cut(s) | 63 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 101 |
| BseMII | CTCAG | 1 cut(s) | 132 |
| BseXI | GCAGC | 1 cut(s) | 22 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 101 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 141 |
| BspCNI | CTCAG | 1 cut(s) | 133 |
| BstDEI | CTNAG | 2 cut(s) | 99, 141 |
| BstF5I | GGATG | 1 cut(s) | 63 |
| BstV1I | GCAGC | 1 cut(s) | 22 |
| BtsCI | GGATG | 1 cut(s) | 63 |
| CviJI | RGCY | 4 cut(s) | 13, 69, 94, 120 |
| CviKI_1 | RGCY | 4 cut(s) | 13, 69, 94, 120 |
| DdeI | CTNAG | 2 cut(s) | 99, 141 |
| Eco57I | CTGAAG | 1 cut(s) | 35 |
| FaiI | YATR | 2 cut(s) | 176, 178 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 151, 183 |
| Fnu4HI | GCNGC | 1 cut(s) | 11 |
| FokI | GGATG | 1 cut(s) | 70 |
| Fsp4HI | GCNGC | 1 cut(s) | 11 |
| FspBI | CTAG | 1 cut(s) | 181 |
| GluI | GCNGC | 1 cut(s) | 11 |
| GsuI | CTGGAG | 1 cut(s) | 65 |
| Hpy188III | TCNNGA | 1 cut(s) | 44 |
| HpyAV | CCTTC | 1 cut(s) | 105 |
| HpyF3I | CTNAG | 2 cut(s) | 99, 141 |
| LpnPI | CCDG | 1 cut(s) | 29 |
| Lsp1109I | GCAGC | 1 cut(s) | 22 |
| MaeI | CTAG | 1 cut(s) | 181 |
| MboII | GAAGA | 2 cut(s) | 28, 123 |
| MnlI | CCTC | 4 cut(s) | 21, 48, 79, 118 |
| MseI | TTAA | 1 cut(s) | 129 |
| MspA1I | CMGCKG | 1 cut(s) | 13 |
| PkrI | GCNGC | 1 cut(s) | 12 |
| PvuII | CAGCTG | 1 cut(s) | 13 |
| SaqAI | TTAA | 1 cut(s) | 129 |
| SatI | GCNGC | 1 cut(s) | 11 |
| SetI | ASST | 3 cut(s) | 15, 71, 129 |
| SgeI | CNNG | 5 cut(s) | 56, 64, 103, 118, 158 |
| SspMI | CTAG | 1 cut(s) | 181 |
| Tru1I | TTAA | 1 cut(s) | 129 |
| Tru9I | TTAA | 1 cut(s) | 129 |
| TseI | GCWGC | 1 cut(s) | 10 |
| XspI | CTAG | 1 cut(s) | 181 |