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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AflIII | ACRYGT | 1 cut(s) | 150 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 151, 183 |
| AluBI | AGCT | 3 cut(s) | 32, 115, 122 |
| AluI | AGCT | 3 cut(s) | 32, 115, 122 |
| ApeKI | GCWGC | 2 cut(s) | 29, 122 |
| AseI | ATTAAT | 2 cut(s) | 234, 303 |
| BbvI | GCAGC | 2 cut(s) | 41, 109 |
| BccI | CCATC | 2 cut(s) | 47, 239 |
| BfaI | CTAG | 1 cut(s) | 119 |
| BisI | GCNGC | 2 cut(s) | 30, 123 |
| BlsI | GCNGC | 2 cut(s) | 31, 124 |
| BmsI | GCATC | 1 cut(s) | 127 |
| BpuEI | CTTGAG | 1 cut(s) | 203 |
| BseGI | GGATG | 1 cut(s) | 62 |
| BseXI | GCAGC | 2 cut(s) | 41, 109 |
| BslFI | GGGAC | 1 cut(s) | 265 |
| BsmFI | GGGAC | 1 cut(s) | 265 |
| BstF5I | GGATG | 1 cut(s) | 62 |
| BstNSI | RCATGY | 1 cut(s) | 154 |
| BstV1I | GCAGC | 2 cut(s) | 41, 109 |
| BtsCI | GGATG | 1 cut(s) | 62 |
| CviAII | CATG | 2 cut(s) | 151, 198 |
| CviJI | RGCY | 5 cut(s) | 32, 65, 115, 122, 208 |
| CviKI_1 | RGCY | 5 cut(s) | 32, 65, 115, 122, 208 |
| FaeI | CATG | 2 cut(s) | 154, 201 |
| FaiI | YATR | 9 cut(s) | 18, 27, 103, 105, 107, 152, 199, 224, 269 |
| FaqI | GGGAC | 1 cut(s) | 265 |
| FatI | CATG | 2 cut(s) | 150, 197 |
| Fnu4HI | GCNGC | 2 cut(s) | 30, 123 |
| FokI | GGATG | 1 cut(s) | 69 |
| Fsp4HI | GCNGC | 2 cut(s) | 30, 123 |
| FspBI | CTAG | 1 cut(s) | 119 |
| GluI | GCNGC | 2 cut(s) | 30, 123 |
| Hin1II | CATG | 2 cut(s) | 154, 201 |
| HindIII | AAGCTT | 1 cut(s) | 113 |
| HinfI | GANTC | 1 cut(s) | 194 |
| Hpy188I | TCNGA | 1 cut(s) | 274 |
| Hpy188III | TCNNGA | 1 cut(s) | 50 |
| HpyCH4V | TGCA | 1 cut(s) | 29 |
| Hsp92II | CATG | 2 cut(s) | 154, 201 |
| LmnI | GCTCC | 1 cut(s) | 37 |
| Lsp1109I | GCAGC | 2 cut(s) | 41, 109 |
| LweI | GCATC | 1 cut(s) | 127 |
| MaeI | CTAG | 1 cut(s) | 119 |
| MluCI | AATT | 1 cut(s) | 304 |
| MlyI | GAGTC | 1 cut(s) | 188 |
| MmeI | TCCRAC | 1 cut(s) | 52 |
| MnlI | CCTC | 3 cut(s) | 3, 220, 286 |
| MseI | TTAA | 5 cut(s) | 6, 93, 213, 234, 303 |
| NlaIII | CATG | 2 cut(s) | 154, 201 |
| NspI | RCATGY | 1 cut(s) | 154 |
| PciI | ACATGT | 1 cut(s) | 150 |
| PkrI | GCNGC | 2 cut(s) | 31, 124 |
| PleI | GAGTC | 1 cut(s) | 188 |
| PpsI | GAGTC | 1 cut(s) | 188 |
| PscI | ACATGT | 1 cut(s) | 150 |
| PshBI | ATTAAT | 2 cut(s) | 234, 303 |
| SaqAI | TTAA | 5 cut(s) | 6, 93, 213, 234, 303 |
| SatI | GCNGC | 2 cut(s) | 30, 123 |
| SchI | GAGTC | 1 cut(s) | 188 |
| SetI | ASST | 3 cut(s) | 34, 117, 124 |
| SfaNI | GCATC | 1 cut(s) | 127 |
| SgeI | CNNG | 7 cut(s) | 48, 62, 131, 163, 194, 210, 216 |
| SmlI | CTYRAG | 1 cut(s) | 182 |
| SmoI | CTYRAG | 1 cut(s) | 182 |
| Sse9I | AATT | 1 cut(s) | 304 |
| SspMI | CTAG | 1 cut(s) | 119 |
| TasI | AATT | 1 cut(s) | 304 |
| Tru1I | TTAA | 5 cut(s) | 6, 93, 213, 234, 303 |
| Tru9I | TTAA | 5 cut(s) | 6, 93, 213, 234, 303 |
| TseI | GCWGC | 2 cut(s) | 29, 122 |
| TspDTI | ATGAA | 3 cut(s) | 90, 211, 256 |
| VspI | ATTAAT | 2 cut(s) | 234, 303 |
| XceI | RCATGY | 1 cut(s) | 154 |
| XspI | CTAG | 1 cut(s) | 119 |