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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 79 |
| AgsI | TTSAA | 1 cut(s) | 176 |
| AlwI | GGATC | 1 cut(s) | 79 |
| BbsI | GAAGAC | 1 cut(s) | 131 |
| BfaI | CTAG | 2 cut(s) | 57, 191 |
| BpiI | GAAGAC | 1 cut(s) | 131 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 230 |
| BseRI | GAGGAG | 2 cut(s) | 78, 251 |
| Bsp143I | GATC | 2 cut(s) | 53, 71 |
| BspPI | GGATC | 1 cut(s) | 79 |
| BssMI | GATC | 2 cut(s) | 53, 71 |
| Bst4CI | ACNGT | 1 cut(s) | 108 |
| BstKTI | GATC | 2 cut(s) | 56, 74 |
| BstMBI | GATC | 2 cut(s) | 53, 71 |
| BstV2I | GAAGAC | 1 cut(s) | 131 |
| BstX2I | RGATCY | 1 cut(s) | 71 |
| BstYI | RGATCY | 1 cut(s) | 71 |
| CviAII | CATG | 1 cut(s) | 221 |
| DpnI | GATC | 2 cut(s) | 55, 73 |
| DpnII | GATC | 2 cut(s) | 53, 71 |
| FaeI | CATG | 1 cut(s) | 224 |
| FaiI | YATR | 3 cut(s) | 15, 198, 222 |
| FatI | CATG | 1 cut(s) | 220 |
| FspBI | CTAG | 2 cut(s) | 57, 191 |
| Hin1II | CATG | 1 cut(s) | 224 |
| Hpy188III | TCNNGA | 2 cut(s) | 143, 191 |
| HpyAV | CCTTC | 1 cut(s) | 122 |
| HpyCH4III | ACNGT | 1 cut(s) | 108 |
| Hsp92II | CATG | 1 cut(s) | 224 |
| Kzo9I | GATC | 2 cut(s) | 53, 71 |
| MaeI | CTAG | 2 cut(s) | 57, 191 |
| MalI | GATC | 2 cut(s) | 55, 73 |
| MboI | GATC | 2 cut(s) | 53, 71 |
| MboII | GAAGA | 3 cut(s) | 66, 131, 236 |
| MflI | RGATCY | 1 cut(s) | 71 |
| MnlI | CCTC | 4 cut(s) | 56, 199, 229, 232 |
| MseI | TTAA | 1 cut(s) | 250 |
| NdeII | GATC | 2 cut(s) | 53, 71 |
| NlaIII | CATG | 1 cut(s) | 224 |
| PsuI | RGATCY | 1 cut(s) | 71 |
| SaqAI | TTAA | 1 cut(s) | 250 |
| Sau3AI | GATC | 2 cut(s) | 53, 71 |
| SgeI | CNNG | 9 cut(s) | 33, 51, 69, 102, 123, 131, 155, 203, 233 |
| SspMI | CTAG | 2 cut(s) | 57, 191 |
| TaaI | ACNGT | 1 cut(s) | 108 |
| Tru1I | TTAA | 1 cut(s) | 250 |
| Tru9I | TTAA | 1 cut(s) | 250 |
| TspDTI | ATGAA | 3 cut(s) | 141, 183, 237 |
| XbaI | TCTAGA | 1 cut(s) | 190 |
| XspI | CTAG | 2 cut(s) | 57, 191 |