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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 494 |
| Acc16I | TGCGCA | 1 cut(s) | 999 |
| Acc36I | ACCTGC | 1 cut(s) | 529 |
| AccB1I | GGYRCC | 1 cut(s) | 309 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 296, 744 |
| AccIII | TCCGGA | 1 cut(s) | 959 |
| AciI | CCGC | 3 cut(s) | 786, 948, 951 |
| AclWI | GGATC | 4 cut(s) | 670, 689, 716, 802 |
| AcsI | RAATTY | 3 cut(s) | 557, 583, 775 |
| AcuI | CTGAAG | 2 cut(s) | 212, 281 |
| AfaI | GTAC | 2 cut(s) | 540, 1018 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 296, 607, 628, 744 |
| AgsI | TTSAA | 5 cut(s) | 232, 530, 589, 685, 890 |
| AjiI | CACGTC | 1 cut(s) | 823 |
| AjnI | CCWGG | 2 cut(s) | 601, 893 |
| AleI | CACNNNNGTG | 1 cut(s) | 51 |
| Alw21I | GWGCWC | 2 cut(s) | 48, 259 |
| Alw26I | GTCTC | 1 cut(s) | 229 |
| AlwI | GGATC | 4 cut(s) | 670, 689, 716, 802 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1020 |
| Aor13HI | TCCGGA | 1 cut(s) | 959 |
| AoxI | GGCC | 2 cut(s) | 90, 287 |
| ApeKI | GCWGC | 2 cut(s) | 97, 995 |
| ApoI | RAATTY | 3 cut(s) | 557, 583, 775 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 432, 659 |
| AspLEI | GCGC | 1 cut(s) | 1000 |
| AspS9I | GGNCC | 3 cut(s) | 90, 486, 619 |
| AsuC2I | CCSGG | 1 cut(s) | 699 |
| AsuHPI | GGTGA | 4 cut(s) | 37, 622, 655, 937 |
| AsuNHI | GCTAGC | 1 cut(s) | 733 |
| AvaII | GGWCC | 2 cut(s) | 486, 619 |
| BanI | GGYRCC | 1 cut(s) | 309 |
| BbsI | GAAGAC | 2 cut(s) | 35, 825 |
| Bbv12I | GWGCWC | 2 cut(s) | 48, 259 |
| BbvCI | CCTCAGC | 1 cut(s) | 852 |
| BbvI | GCAGC | 2 cut(s) | 109, 982 |
| BccI | CCATC | 4 cut(s) | 259, 299, 400, 785 |
| BceAI | ACGGC | 1 cut(s) | 1008 |
| BciT130I | CCWGG | 2 cut(s) | 603, 895 |
| BcnI | CCSGG | 1 cut(s) | 699 |
| BcoDI | GTCTC | 1 cut(s) | 229 |
| BfaI | CTAG | 6 cut(s) | 57, 104, 726, 734, 939, 966 |
| BfuAI | ACCTGC | 1 cut(s) | 529 |
| BglII | AGATCT | 1 cut(s) | 883 |
| BisI | GCNGC | 3 cut(s) | 98, 951, 996 |
| BlsI | GCNGC | 3 cut(s) | 99, 952, 997 |
| Bme1390I | CCNGG | 3 cut(s) | 603, 699, 895 |
| Bme18I | GGWCC | 2 cut(s) | 486, 619 |
| BmgBI | CACGTC | 1 cut(s) | 823 |
| BmgT120I | GGNCC | 3 cut(s) | 90, 486, 619 |
| BmiI | GGNNCC | 2 cut(s) | 91, 311 |
| BmrFI | CCNGG | 3 cut(s) | 603, 699, 895 |
| BmsI | GCATC | 1 cut(s) | 119 |
| BmtI | GCTAGC | 1 cut(s) | 737 |
| BpiI | GAAGAC | 2 cut(s) | 35, 825 |
| Bpu10I | CCTNAGC | 1 cut(s) | 852 |
| BpuEI | CTTGAG | 1 cut(s) | 478 |
| BpuMI | CCSGG | 1 cut(s) | 699 |
| BsaJI | CCNNGG | 2 cut(s) | 295, 601 |
| BsaWI | WCCGGW | 1 cut(s) | 959 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 954, 984 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 296, 607, 628, 744 |
| Bse1I | ACTGG | 1 cut(s) | 290 |
| BseAI | TCCGGA | 1 cut(s) | 959 |
| BseBI | CCWGG | 2 cut(s) | 603, 895 |
| BseDI | CCNNGG | 2 cut(s) | 295, 601 |
| BseGI | GGATG | 3 cut(s) | 287, 367, 475 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 296, 607, 628, 744 |
| BseMII | CTCAG | 2 cut(s) | 843, 909 |
| BseNI | ACTGG | 1 cut(s) | 290 |
| BseRI | GAGGAG | 1 cut(s) | 167 |
| BseXI | GCAGC | 2 cut(s) | 109, 982 |
| BseYI | CCCAGC | 1 cut(s) | 479 |
| BshFI | GGCC | 2 cut(s) | 92, 289 |
| BshNI | GGYRCC | 1 cut(s) | 309 |
| BsiHKAI | GWGCWC | 2 cut(s) | 48, 259 |
| BsiSI | CCGG | 3 cut(s) | 699, 933, 960 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 296, 607, 628, 744 |
| BsmAI | GTCTC | 1 cut(s) | 229 |
| BsmI | GAATGC | 1 cut(s) | 1000 |
| BsnI | GGCC | 2 cut(s) | 92, 289 |
| Bsp1286I | GDGCHC | 2 cut(s) | 48, 259 |
| Bsp13I | TCCGGA | 1 cut(s) | 959 |
| Bsp143I | GATC | 8 cut(s) | 187, 675, 694, 721, 756, 768, 794, 883 |
| BspACI | CCGC | 3 cut(s) | 786, 948, 951 |
| BspANI | GGCC | 2 cut(s) | 92, 289 |
| BspCNI | CTCAG | 2 cut(s) | 844, 910 |
| BspEI | TCCGGA | 1 cut(s) | 959 |
| BspHI | TCATGA | 1 cut(s) | 859 |
| BspLI | GGNNCC | 2 cut(s) | 91, 311 |
| BspMI | ACCTGC | 1 cut(s) | 529 |
| BspOI | GCTAGC | 1 cut(s) | 737 |
| BspPI | GGATC | 4 cut(s) | 670, 689, 716, 802 |
| BspT107I | GGYRCC | 1 cut(s) | 309 |
| BsrI | ACTGG | 1 cut(s) | 290 |
| BssECI | CCNNGG | 2 cut(s) | 295, 601 |
| BssMI | GATC | 8 cut(s) | 187, 675, 694, 721, 756, 768, 794, 883 |
| BssT1I | CCWWGG | 1 cut(s) | 295 |
| Bst2UI | CCWGG | 2 cut(s) | 603, 895 |
| Bst4CI | ACNGT | 5 cut(s) | 332, 339, 422, 444, 875 |
| BstC8I | GCNNGC | 2 cut(s) | 68, 735 |
| BstDEI | CTNAG | 2 cut(s) | 852, 918 |
| BstF5I | GGATG | 3 cut(s) | 287, 367, 475 |
| BstHHI | GCGC | 1 cut(s) | 1000 |
| BstKTI | GATC | 8 cut(s) | 190, 678, 697, 724, 759, 771, 797, 886 |
| BstMAI | GTCTC | 1 cut(s) | 229 |
| BstMBI | GATC | 8 cut(s) | 187, 675, 694, 721, 756, 768, 794, 883 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 76, 739, 848, 971 |
| BstNI | CCWGG | 2 cut(s) | 603, 895 |
| BstNSI | RCATGY | 1 cut(s) | 636 |
| BstSCI | CCNGG | 3 cut(s) | 601, 697, 893 |
| BstV1I | GCAGC | 2 cut(s) | 109, 982 |
| BstV2I | GAAGAC | 2 cut(s) | 35, 825 |
| BstX2I | RGATCY | 2 cut(s) | 721, 883 |
| BstYI | RGATCY | 2 cut(s) | 721, 883 |
| BsuRI | GGCC | 2 cut(s) | 92, 289 |
| BtrI | CACGTC | 1 cut(s) | 823 |
| BtsCI | GGATG | 3 cut(s) | 287, 367, 475 |
| BtsI | GCAGTG | 1 cut(s) | 838 |
| BtsIMutI | CAGTG | 3 cut(s) | 257, 838, 880 |
| BveI | ACCTGC | 1 cut(s) | 529 |
| Cac8I | GCNNGC | 2 cut(s) | 68, 735 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1020 |
| CciI | TCATGA | 1 cut(s) | 859 |
| CfoI | GCGC | 1 cut(s) | 1000 |
| Cfr13I | GGNCC | 3 cut(s) | 90, 486, 619 |
| CseI | GACGC | 1 cut(s) | 889 |
| Csp6I | GTAC | 2 cut(s) | 539, 1017 |
| CviAII | CATG | 6 cut(s) | 8, 22, 378, 633, 860, 869 |
| CviQI | GTAC | 2 cut(s) | 539, 1017 |
| DdeI | CTNAG | 2 cut(s) | 852, 918 |
| DpnI | GATC | 8 cut(s) | 189, 677, 696, 723, 758, 770, 796, 885 |
| DpnII | GATC | 8 cut(s) | 187, 675, 694, 721, 756, 768, 794, 883 |
| DraI | TTTAAA | 1 cut(s) | 318 |
| EciI | GGCGGA | 1 cut(s) | 775 |
| Eco130I | CCWWGG | 1 cut(s) | 295 |
| Eco47I | GGWCC | 2 cut(s) | 486, 619 |
| Eco57I | CTGAAG | 2 cut(s) | 212, 281 |
| EcoO109I | RGGNCCY | 1 cut(s) | 90 |
| EcoRII | CCWGG | 2 cut(s) | 601, 893 |
| EcoT14I | CCWWGG | 1 cut(s) | 295 |
| ErhI | CCWWGG | 1 cut(s) | 295 |
| FaeI | CATG | 6 cut(s) | 11, 25, 381, 636, 863, 872 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 185, 217 |
| FatI | CATG | 6 cut(s) | 7, 21, 377, 632, 859, 868 |
| FauNDI | CATATG | 1 cut(s) | 706 |
| Fnu4HI | GCNGC | 3 cut(s) | 98, 951, 996 |
| FokI | GGATG | 3 cut(s) | 294, 374, 482 |
| Fsp4HI | GCNGC | 3 cut(s) | 98, 951, 996 |
| FspBI | CTAG | 6 cut(s) | 57, 104, 726, 734, 939, 966 |
| FspI | TGCGCA | 1 cut(s) | 999 |
| GlaI | GCGC | 1 cut(s) | 999 |
| GluI | GCNGC | 3 cut(s) | 98, 951, 996 |
| GsaI | CCCAGC | 1 cut(s) | 483 |
| HaeIII | GGCC | 2 cut(s) | 92, 289 |
| HapII | CCGG | 3 cut(s) | 699, 933, 960 |
| HgaI | GACGC | 1 cut(s) | 889 |
| HhaI | GCGC | 1 cut(s) | 1000 |
| Hin1II | CATG | 6 cut(s) | 11, 25, 381, 636, 863, 872 |
| Hin6I | GCGC | 1 cut(s) | 998 |
| HinP1I | GCGC | 1 cut(s) | 998 |
| HincII | GTYRAC | 1 cut(s) | 169 |
| HindII | GTYRAC | 1 cut(s) | 169 |
| HindIII | AAGCTT | 1 cut(s) | 68 |
| HinfI | GANTC | 1 cut(s) | 942 |
| HpaII | CCGG | 3 cut(s) | 699, 933, 960 |
| HphI | GGTGA | 4 cut(s) | 37, 622, 655, 937 |
| Hpy166II | GTNNAC | 4 cut(s) | 169, 335, 486, 579 |
| Hpy188I | TCNGA | 2 cut(s) | 192, 773 |
| Hpy188III | TCNNGA | 9 cut(s) | 41, 104, 212, 232, 437, 530, 682, 860, 960 |
| Hpy8I | GTNNAC | 4 cut(s) | 169, 335, 486, 579 |
| Hpy99I | CGWCG | 3 cut(s) | 905, 908, 994 |
| HpyAV | CCTTC | 3 cut(s) | 445, 610, 723 |
| HpyCH4III | ACNGT | 5 cut(s) | 332, 339, 422, 444, 875 |
| HpyCH4IV | ACGT | 1 cut(s) | 822 |
| HpyCH4V | TGCA | 5 cut(s) | 4, 97, 131, 548, 638 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 76, 739, 848, 971 |
| HpyF3I | CTNAG | 2 cut(s) | 852, 918 |
| HpySE526I | ACGT | 1 cut(s) | 822 |
| Hsp92II | CATG | 6 cut(s) | 11, 25, 381, 636, 863, 872 |
| HspAI | GCGC | 1 cut(s) | 998 |
| Kpn2I | TCCGGA | 1 cut(s) | 959 |
| Kzo9I | GATC | 8 cut(s) | 187, 675, 694, 721, 756, 768, 794, 883 |
| LmnI | GCTCC | 8 cut(s) | 154, 254, 476, 701, 747, 788, 897, 962 |
| Lsp1109I | GCAGC | 2 cut(s) | 109, 982 |
| LweI | GCATC | 1 cut(s) | 119 |
| MaeI | CTAG | 6 cut(s) | 57, 104, 726, 734, 939, 966 |
| MaeII | ACGT | 1 cut(s) | 822 |
| MaeIII | GTNAC | 4 cut(s) | 197, 219, 516, 943 |
| MalI | GATC | 8 cut(s) | 189, 677, 696, 723, 758, 770, 796, 885 |
| MboI | GATC | 8 cut(s) | 187, 675, 694, 721, 756, 768, 794, 883 |
| MboII | GAAGA | 4 cut(s) | 40, 274, 830, 878 |
| MfeI | CAATTG | 1 cut(s) | 183 |
| MflI | RGATCY | 2 cut(s) | 721, 883 |
| MhlI | GDGCHC | 2 cut(s) | 48, 259 |
| MluCI | AATT | 5 cut(s) | 125, 183, 557, 583, 775 |
| MlyI | GAGTC | 1 cut(s) | 951 |
| MroI | TCCGGA | 1 cut(s) | 959 |
| MroXI | GAANNNNTTC | 2 cut(s) | 432, 659 |
| MseI | TTAA | 4 cut(s) | 159, 317, 765, 779 |
| MslI | CAYNNNNRTG | 3 cut(s) | 20, 51, 873 |
| MspA1I | CMGCKG | 2 cut(s) | 350, 1008 |
| MspI | CCGG | 3 cut(s) | 699, 933, 960 |
| MspR9I | CCNGG | 3 cut(s) | 603, 699, 895 |
| MunI | CAATTG | 1 cut(s) | 183 |
| Mva1269I | GAATGC | 1 cut(s) | 1000 |
| MvaI | CCWGG | 2 cut(s) | 603, 895 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 76, 739, 848, 971 |
| NciI | CCSGG | 1 cut(s) | 699 |
| NdeI | CATATG | 1 cut(s) | 706 |
| NdeII | GATC | 8 cut(s) | 187, 675, 694, 721, 756, 768, 794, 883 |
| NheI | GCTAGC | 1 cut(s) | 733 |
| NlaIII | CATG | 6 cut(s) | 11, 25, 381, 636, 863, 872 |
| NlaIV | GGNNCC | 2 cut(s) | 91, 311 |
| NmuCI | GTSAC | 2 cut(s) | 197, 943 |
| NsbI | TGCGCA | 1 cut(s) | 999 |
| NspI | RCATGY | 1 cut(s) | 636 |
| OliI | CACNNNNGTG | 1 cut(s) | 51 |
| PagI | TCATGA | 1 cut(s) | 859 |
| PctI | GAATGC | 1 cut(s) | 1000 |
| PdmI | GAANNNNTTC | 2 cut(s) | 432, 659 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 296, 744 |
| PfoI | TCCNGGA | 1 cut(s) | 697 |
| PkrI | GCNGC | 3 cut(s) | 99, 952, 997 |
| PleI | GAGTC | 1 cut(s) | 950 |
| PpsI | GAGTC | 1 cut(s) | 950 |
| PsiI | TTATAA | 1 cut(s) | 494 |
| Psp6I | CCWGG | 2 cut(s) | 601, 893 |
| PspFI | CCCAGC | 1 cut(s) | 479 |
| PspGI | CCWGG | 2 cut(s) | 601, 893 |
| PspN4I | GGNNCC | 2 cut(s) | 91, 311 |
| PspPI | GGNCC | 3 cut(s) | 90, 486, 619 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1020 |
| PsuI | RGATCY | 2 cut(s) | 721, 883 |
| PvuII | CAGCTG | 2 cut(s) | 350, 1008 |
| RsaI | GTAC | 2 cut(s) | 540, 1018 |
| RsaNI | GTAC | 2 cut(s) | 539, 1017 |
| RseI | CAYNNNNRTG | 3 cut(s) | 20, 51, 873 |
| SaqAI | TTAA | 4 cut(s) | 159, 317, 765, 779 |
| SatI | GCNGC | 3 cut(s) | 98, 951, 996 |
| Sau3AI | GATC | 8 cut(s) | 187, 675, 694, 721, 756, 768, 794, 883 |
| Sau96I | GGNCC | 3 cut(s) | 90, 486, 619 |
| SchI | GAGTC | 1 cut(s) | 951 |
| ScrFI | CCNGG | 3 cut(s) | 603, 699, 895 |
| SduI | GDGCHC | 2 cut(s) | 48, 259 |
| SfaNI | GCATC | 1 cut(s) | 119 |
| SinI | GGWCC | 2 cut(s) | 486, 619 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 20, 51, 873 |
| SmlI | CTYRAG | 1 cut(s) | 457 |
| SmoI | CTYRAG | 1 cut(s) | 457 |
| Sse9I | AATT | 5 cut(s) | 125, 183, 557, 583, 775 |
| SsiI | CCGC | 3 cut(s) | 786, 948, 951 |
| SspMI | CTAG | 6 cut(s) | 57, 104, 726, 734, 939, 966 |
| StyD4I | CCNGG | 3 cut(s) | 601, 697, 893 |
| StyI | CCWWGG | 1 cut(s) | 295 |
| TaaI | ACNGT | 5 cut(s) | 332, 339, 422, 444, 875 |
| TaiI | ACGT | 1 cut(s) | 825 |
| TaqI | TCGA | 1 cut(s) | 816 |
| TasI | AATT | 5 cut(s) | 125, 183, 557, 583, 775 |
| TauI | GCSGC | 1 cut(s) | 953 |
| Tru1I | TTAA | 4 cut(s) | 159, 317, 765, 779 |
| Tru9I | TTAA | 4 cut(s) | 159, 317, 765, 779 |
| TscAI | CASTG | 3 cut(s) | 264, 838, 880 |
| TseFI | GTSAC | 2 cut(s) | 197, 943 |
| TseI | GCWGC | 2 cut(s) | 97, 995 |
| Tsp45I | GTSAC | 2 cut(s) | 197, 943 |
| TspDTI | ATGAA | 4 cut(s) | 629, 668, 852, 861 |
| TspRI | CASTG | 3 cut(s) | 264, 838, 880 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 296, 744 |
| VpaK11BI | GGWCC | 2 cut(s) | 486, 619 |
| XapI | RAATTY | 3 cut(s) | 557, 583, 775 |
| XbaI | TCTAGA | 1 cut(s) | 103 |
| XceI | RCATGY | 1 cut(s) | 636 |
| XmnI | GAANNNNTTC | 2 cut(s) | 432, 659 |
| XspI | CTAG | 6 cut(s) | 57, 104, 726, 734, 939, 966 |