RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 30 |
| AcsI | RAATTY | 3 cut(s) | 138, 210, 323 |
| AcyI | GRCGYC | 1 cut(s) | 389 |
| AfaI | GTAC | 3 cut(s) | 8, 245, 366 |
| AgsI | TTSAA | 4 cut(s) | 179, 290, 346, 398 |
| AluBI | AGCT | 1 cut(s) | 471 |
| AluI | AGCT | 1 cut(s) | 471 |
| Alw26I | GTCTC | 1 cut(s) | 266 |
| ApoI | RAATTY | 3 cut(s) | 138, 210, 323 |
| AseI | ATTAAT | 2 cut(s) | 45, 131 |
| AsuHPI | GGTGA | 1 cut(s) | 311 |
| BcoDI | GTCTC | 1 cut(s) | 266 |
| BfaI | CTAG | 1 cut(s) | 420 |
| BsaHI | GRCGYC | 1 cut(s) | 389 |
| BsaJI | CCNNGG | 1 cut(s) | 478 |
| BseDI | CCNNGG | 1 cut(s) | 478 |
| BseRI | GAGGAG | 2 cut(s) | 238, 317 |
| BsmAI | GTCTC | 1 cut(s) | 266 |
| BsmBI | CGTCTC | 1 cut(s) | 266 |
| BssECI | CCNNGG | 1 cut(s) | 478 |
| BssNI | GRCGYC | 1 cut(s) | 389 |
| BssT1I | CCWWGG | 1 cut(s) | 478 |
| Bst4CI | ACNGT | 1 cut(s) | 362 |
| BstACI | GRCGYC | 1 cut(s) | 389 |
| BstDEI | CTNAG | 1 cut(s) | 384 |
| BstMAI | GTCTC | 1 cut(s) | 266 |
| BstNSI | RCATGY | 2 cut(s) | 71, 97 |
| BtsI | GCAGTG | 1 cut(s) | 235 |
| BtsIMutI | CAGTG | 1 cut(s) | 235 |
| CseI | GACGC | 1 cut(s) | 397 |
| Csp6I | GTAC | 3 cut(s) | 7, 244, 365 |
| CviAII | CATG | 6 cut(s) | 37, 68, 94, 308, 355, 436 |
| CviJI | RGCY | 3 cut(s) | 319, 418, 471 |
| CviKI_1 | RGCY | 3 cut(s) | 319, 418, 471 |
| CviQI | GTAC | 3 cut(s) | 7, 244, 365 |
| DdeI | CTNAG | 1 cut(s) | 384 |
| Eco130I | CCWWGG | 1 cut(s) | 478 |
| EcoT14I | CCWWGG | 1 cut(s) | 478 |
| EcoT22I | ATGCAT | 1 cut(s) | 95 |
| ErhI | CCWWGG | 1 cut(s) | 478 |
| Esp3I | CGTCTC | 1 cut(s) | 266 |
| FaeI | CATG | 6 cut(s) | 40, 71, 97, 311, 358, 439 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 455, 487 |
| FatI | CATG | 6 cut(s) | 36, 67, 93, 307, 354, 435 |
| FspBI | CTAG | 1 cut(s) | 420 |
| HgaI | GACGC | 1 cut(s) | 397 |
| Hin1I | GRCGYC | 1 cut(s) | 389 |
| Hin1II | CATG | 6 cut(s) | 40, 71, 97, 311, 358, 439 |
| HindIII | AAGCTT | 1 cut(s) | 469 |
| HinfI | GANTC | 1 cut(s) | 218 |
| HphI | GGTGA | 1 cut(s) | 311 |
| Hpy166II | GTNNAC | 1 cut(s) | 365 |
| Hpy188III | TCNNGA | 1 cut(s) | 179 |
| Hpy8I | GTNNAC | 1 cut(s) | 365 |
| HpyAV | CCTTC | 1 cut(s) | 284 |
| HpyCH4III | ACNGT | 1 cut(s) | 362 |
| HpyCH4V | TGCA | 5 cut(s) | 16, 36, 93, 225, 358 |
| HpyF3I | CTNAG | 1 cut(s) | 384 |
| Hsp92I | GRCGYC | 1 cut(s) | 389 |
| Hsp92II | CATG | 6 cut(s) | 40, 71, 97, 311, 358, 439 |
| LmnI | GCTCC | 1 cut(s) | 304 |
| LpnPI | CCDG | 4 cut(s) | 152, 226, 393, 467 |
| MaeI | CTAG | 1 cut(s) | 420 |
| MaeIII | GTNAC | 2 cut(s) | 426, 448 |
| MboII | GAAGA | 3 cut(s) | 62, 173, 358 |
| MluCI | AATT | 5 cut(s) | 25, 138, 186, 210, 323 |
| MnlI | CCTC | 2 cut(s) | 259, 295 |
| Mph1103I | ATGCAT | 1 cut(s) | 95 |
| MseI | TTAA | 3 cut(s) | 45, 131, 473 |
| MslI | CAYNNNNRTG | 1 cut(s) | 256 |
| NlaIII | CATG | 6 cut(s) | 40, 71, 97, 311, 358, 439 |
| NsiI | ATGCAT | 1 cut(s) | 95 |
| NspI | RCATGY | 2 cut(s) | 71, 97 |
| PfeI | GAWTC | 1 cut(s) | 218 |
| PshBI | ATTAAT | 2 cut(s) | 45, 131 |
| PsiI | TTATAA | 1 cut(s) | 30 |
| RsaI | GTAC | 3 cut(s) | 8, 245, 366 |
| RsaNI | GTAC | 3 cut(s) | 7, 244, 365 |
| RseI | CAYNNNNRTG | 1 cut(s) | 256 |
| SaqAI | TTAA | 3 cut(s) | 45, 131, 473 |
| SetI | ASST | 2 cut(s) | 412, 473 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 256 |
| Sse9I | AATT | 5 cut(s) | 25, 138, 186, 210, 323 |
| SspMI | CTAG | 1 cut(s) | 420 |
| StyI | CCWWGG | 1 cut(s) | 478 |
| TaaI | ACNGT | 1 cut(s) | 362 |
| TaqI | TCGA | 1 cut(s) | 404 |
| TasI | AATT | 5 cut(s) | 25, 138, 186, 210, 323 |
| TatI | WGTACW | 1 cut(s) | 6 |
| TfiI | GAWTC | 1 cut(s) | 218 |
| Tru1I | TTAA | 3 cut(s) | 45, 131, 473 |
| Tru9I | TTAA | 3 cut(s) | 45, 131, 473 |
| TscAI | CASTG | 1 cut(s) | 242 |
| TspDTI | ATGAA | 4 cut(s) | 63, 149, 424, 428 |
| TspRI | CASTG | 1 cut(s) | 242 |
| VspI | ATTAAT | 2 cut(s) | 45, 131 |
| XapI | RAATTY | 3 cut(s) | 138, 210, 323 |
| XceI | RCATGY | 2 cut(s) | 71, 97 |
| XspI | CTAG | 1 cut(s) | 420 |
| Zsp2I | ATGCAT | 1 cut(s) | 95 |