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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 128 |
| AgsI | TTSAA | 4 cut(s) | 25, 107, 133, 204 |
| AluBI | AGCT | 4 cut(s) | 15, 31, 38, 178 |
| AluI | AGCT | 4 cut(s) | 15, 31, 38, 178 |
| ApeKI | GCWGC | 3 cut(s) | 79, 172, 175 |
| ApoI | RAATTY | 1 cut(s) | 128 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 128 |
| AspS9I | GGNCC | 1 cut(s) | 18 |
| AvaII | GGWCC | 1 cut(s) | 18 |
| BbvI | GCAGC | 3 cut(s) | 91, 184, 187 |
| BccI | CCATC | 1 cut(s) | 166 |
| BfmI | CTRYAG | 2 cut(s) | 147, 170 |
| BisI | GCNGC | 3 cut(s) | 80, 173, 176 |
| BlsI | GCNGC | 3 cut(s) | 81, 174, 177 |
| Bme18I | GGWCC | 1 cut(s) | 18 |
| BmgT120I | GGNCC | 1 cut(s) | 18 |
| BseGI | GGATG | 1 cut(s) | 55 |
| BseRI | GAGGAG | 1 cut(s) | 68 |
| BseXI | GCAGC | 3 cut(s) | 91, 184, 187 |
| BspMAI | CTGCAG | 1 cut(s) | 174 |
| BstDEI | CTNAG | 1 cut(s) | 138 |
| BstF5I | GGATG | 1 cut(s) | 55 |
| BstSFI | CTRYAG | 2 cut(s) | 147, 170 |
| BstV1I | GCAGC | 3 cut(s) | 91, 184, 187 |
| BtsCI | GGATG | 1 cut(s) | 55 |
| Cfr13I | GGNCC | 1 cut(s) | 18 |
| DdeI | CTNAG | 1 cut(s) | 138 |
| Eco47I | GGWCC | 1 cut(s) | 18 |
| FaiI | YATR | 1 cut(s) | 149 |
| Fnu4HI | GCNGC | 3 cut(s) | 80, 173, 176 |
| FokI | GGATG | 1 cut(s) | 62 |
| Fsp4HI | GCNGC | 3 cut(s) | 80, 173, 176 |
| GluI | GCNGC | 3 cut(s) | 80, 173, 176 |
| HincII | GTYRAC | 1 cut(s) | 186 |
| HindII | GTYRAC | 1 cut(s) | 186 |
| Hpy166II | GTNNAC | 1 cut(s) | 186 |
| Hpy188I | TCNGA | 1 cut(s) | 163 |
| Hpy8I | GTNNAC | 1 cut(s) | 186 |
| HpyAV | CCTTC | 1 cut(s) | 216 |
| HpyCH4V | TGCA | 2 cut(s) | 4, 172 |
| HpyF3I | CTNAG | 1 cut(s) | 138 |
| Lsp1109I | GCAGC | 3 cut(s) | 91, 184, 187 |
| MboII | GAAGA | 1 cut(s) | 79 |
| MluCI | AATT | 2 cut(s) | 97, 128 |
| MnlI | CCTC | 3 cut(s) | 46, 94, 100 |
| MroXI | GAANNNNTTC | 1 cut(s) | 128 |
| MseI | TTAA | 1 cut(s) | 197 |
| PdmI | GAANNNNTTC | 1 cut(s) | 128 |
| PkrI | GCNGC | 3 cut(s) | 81, 174, 177 |
| PspPI | GGNCC | 1 cut(s) | 18 |
| PstI | CTGCAG | 1 cut(s) | 174 |
| SaqAI | TTAA | 1 cut(s) | 197 |
| SatI | GCNGC | 3 cut(s) | 80, 173, 176 |
| Sau96I | GGNCC | 1 cut(s) | 18 |
| SetI | ASST | 7 cut(s) | 17, 23, 33, 40, 92, 180, 227 |
| SfcI | CTRYAG | 2 cut(s) | 147, 170 |
| SgeI | CNNG | 8 cut(s) | 19, 28, 34, 58, 145, 166, 201, 216 |
| SinI | GGWCC | 1 cut(s) | 18 |
| Sse9I | AATT | 2 cut(s) | 97, 128 |
| TasI | AATT | 2 cut(s) | 97, 128 |
| Tru1I | TTAA | 1 cut(s) | 197 |
| Tru9I | TTAA | 1 cut(s) | 197 |
| TseI | GCWGC | 3 cut(s) | 79, 172, 175 |
| TspDTI | ATGAA | 1 cut(s) | 80 |
| VpaK11BI | GGWCC | 1 cut(s) | 18 |
| XapI | RAATTY | 1 cut(s) | 128 |
| XmnI | GAANNNNTTC | 1 cut(s) | 128 |