Rmu_sc0005996.1_g000004

gag-polypeptide of LTR copia-type

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005996.1
OG0000077
Physical Location & Seq
Reverse (-)
22897 .. 24121
1225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005996.1_g000004.1.cds

Sequence Viewer

Length: 729 bp
atgaacttgaacagtgtcttgatgctaactggaaccaactatagagcttggctagattctgtagagaactatatgggaatgcatgagaatatagactattgctttactgaggataagcctgaagagttgaatgaaaagagcaccaagaaagaaactgacctttacaagaagtggcatagatccaatagaatggctaagaagctcattcgtacctctatgtccaagactgtcaggcgaagtatagaagagcctgagctagcatcagattttctggaactcataagtgctaagtttagagaaagtgaaaaaacagaagcagctaggctaaccaaggagtttcatgatttgaagtacatgggttcagggggagttagagagcagatcatgaagatgattaatataaacggcagactcagggagctattgatgggggataaacttatatacatctgtgtggatgaggaagctaggatcaagcaagagaaagaacttgctacagcaataaacctcatagagaagcctaaaagaaaaaagccccagaacaagttcaagcctattagagccataactaagagttcaacagctacagtggccaaggaaaacatgtcatttaggttcaagtgctacttttgcaaaagagtagtagtcgaagaagaccgactgacccgacccgaacccggcagatccgacccgacttttccggccgactgcggcggtctccggccgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2

Protein Analysis

242

Amino Acids

28.22

Weight (kDa)

9.3

Isoelectric Point (pI)

57.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000077)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53670
fragaria_vesca FvH4_1g12751 FvH4_1g13491 FvH4_1g13491 FvH4_1g16302 FvH4_1g16303 FvH4_1g29161 FvH4_1g29941 FvH4_2g02290 FvH4_2g05985 FvH4_2g15841 FvH4_2g18261 FvH4_2g22761 FvH4_2g22761 FvH4_2g22761 FvH4_3g13432 FvH4_3g14801 FvH4_3g28581 FvH4_3g30611 FvH4_3g30611 FvH4_3g30611 FvH4_3g32571 FvH4_3g32571 FvH4_3g32571 FvH4_3g33631 FvH4_3g36663 FvH4_3g36664 FvH4_4g00961 FvH4_4g17672 FvH4_5g13441 FvH4_5g30231 FvH4_5g39263 FvH4_6g06661 FvH4_6g06662 FvH4_6g40841 FvH4_7g00732 FvH4_7g11331 FvH4_7g12361
malus_domestica MD02G1100700.v1.1 MD02G1280000.v1.1 MD03G1045100.v1.1 MD04G1043300.v1.1 MD05G1061800.v1.1 MD05G1118300.v1.1 MD05G1334900.v1.1 MD08G1232100.v1.1 MD10G1202700.v1.1 MD11G1016600.v1.1 MD11G1271900.v1.1 MD15G1002900.v1.1 MD15G1059300.v1.1
prunus_persica Prupe.1G445900_v2.0.a1 Prupe.4G244000_v2.0.a1 Prupe.6G221400_v2.0.a1 Prupe.6G301400_v2.0.a1 Prupe.7G031600_v2.0.a1 Prupe.8G017100_v2.0.a1
pyrus_communis pycom01g08140 pycom01g19130 pycom02g09810 pycom03g12420 pycom05g09140 pycom05g22780 pycom06g17440 pycom07g04850 pycom07g08300 pycom08g09110 pycom08g10760 pycom09g15580 pycom09g18260 pycom09g18400 pycom11g07380 pycom11g20370 pycom12g01630 pycom12g11730 pycom13g21040 pycom14g00290 pycom14g10520 pycom15g16640 pycom15g18720 pycom15g24540 pycom15g26360 pycom16g08100 pycom16g09950 pycom16g11700 pycom16g19690 pycom17g20400
rosa_chinensis RchiOBHm_Chr1g0317481 RchiOBHm_Chr1g0322601 RchiOBHm_Chr1g0324761 RchiOBHm_Chr1g0335001 RchiOBHm_Chr1g0336961 RchiOBHm_Chr1g0345341 RchiOBHm_Chr1g0348501 RchiOBHm_Chr1g0352591 RchiOBHm_Chr1g0354901 RchiOBHm_Chr1g0365141 RchiOBHm_Chr1g0365511 RchiOBHm_Chr1g0368471 RchiOBHm_Chr1g0373471 RchiOBHm_Chr1g0373951 RchiOBHm_Chr1g0375621 RchiOBHm_Chr1g0378491 RchiOBHm_Chr1g0383431 RchiOBHm_Chr2g0086291 RchiOBHm_Chr2g0096681 RchiOBHm_Chr2g0117391 RchiOBHm_Chr2g0121511 RchiOBHm_Chr2g0128511 RchiOBHm_Chr2g0159591 RchiOBHm_Chr2g0173271 RchiOBHm_Chr3g0453771 RchiOBHm_Chr3g0462071 RchiOBHm_Chr3g0467151 RchiOBHm_Chr3g0468531 RchiOBHm_Chr3g0478981 RchiOBHm_Chr3g0488371 RchiOBHm_Chr3g0496001 RchiOBHm_Chr3g0496651 RchiOBHm_Chr4g0390941 RchiOBHm_Chr4g0431251 RchiOBHm_Chr4g0441691 RchiOBHm_Chr5g0001181 RchiOBHm_Chr5g0001671 RchiOBHm_Chr5g0021461 RchiOBHm_Chr5g0045101 RchiOBHm_Chr5g0045461 RchiOBHm_Chr5g0063421 RchiOBHm_Chr5g0068061 RchiOBHm_Chr5g0069541 RchiOBHm_Chr5g0073131 RchiOBHm_Chr5g0076631 RchiOBHm_Chr5g0079181 RchiOBHm_Chr5g0082051 RchiOBHm_Chr6g0248451 RchiOBHm_Chr6g0255561 RchiOBHm_Chr6g0266491 RchiOBHm_Chr6g0268111 RchiOBHm_Chr6g0304521 RchiOBHm_Chr6g0306511 RchiOBHm_Chr7g0181641 RchiOBHm_Chr7g0181791 RchiOBHm_Chr7g0188321 RchiOBHm_Chr7g0194951 RchiOBHm_Chr7g0195071 RchiOBHm_Chr7g0199671 RchiOBHm_Chr7g0204631 RchiOBHm_Chr7g0231771 RchiOBHm_Chr7g0231831 RchiOBHm_Chr7g0242511
rosa_laevigata RLG00000015596 RLG00000021778 RLG00000028187
rosa_multiflora Rmu_co8285397.1_g000001 Rmu_co8301931.1_g000001 Rmu_co8376351.1_g000001 Rmu_co8412043.1_g000001 Rmu_sc0000079.1_g000006 Rmu_sc0000160.1_g000007 Rmu_sc0000208.1_g000006 Rmu_sc0000258.1_g000016 Rmu_sc0000295.1_g000008 Rmu_sc0000302.1_g000025 Rmu_sc0000423.1_g000039 Rmu_sc0000441.1_g000040 Rmu_sc0000538.1_g000074 Rmu_sc0000603.1_g000019 Rmu_sc0000611.1_g000018 Rmu_sc0000729.1_g000002 Rmu_sc0000837.1_g000044 Rmu_sc0000903.1_g000015 Rmu_sc0000924.1_g000029 Rmu_sc0001173.1_g000021 Rmu_sc0001289.1_g000004 Rmu_sc0001347.1_g000005 Rmu_sc0001464.1_g000020 Rmu_sc0001618.1_g000015 Rmu_sc0002014.1_g000002 Rmu_sc0002047.1_g000002 Rmu_sc0002160.1_g000029 Rmu_sc0002310.1_g000011 Rmu_sc0002416.1_g000002 Rmu_sc0002417.1_g000008 Rmu_sc0002445.1_g000008 Rmu_sc0002691.1_g000026 Rmu_sc0002700.1_g000021 Rmu_sc0002748.1_g000032 Rmu_sc0002792.1_g000048 Rmu_sc0002831.1_g000012 Rmu_sc0002928.1_g000025 Rmu_sc0002956.1_g000001 Rmu_sc0003170.1_g000039 Rmu_sc0003232.1_g000024 Rmu_sc0003391.1_g000014 Rmu_sc0003600.1_g000009 Rmu_sc0003630.1_g000064 Rmu_sc0003633.1_g000035 Rmu_sc0003802.1_g000001 Rmu_sc0003881.1_g000001 Rmu_sc0003989.1_g000001 Rmu_sc0004208.1_g000021 Rmu_sc0004815.1_g000004 Rmu_sc0004821.1_g000002 Rmu_sc0005237.1_g000004 Rmu_sc0005638.1_g000006 Rmu_sc0005996.1_g000004 Rmu_sc0006604.1_g000005 Rmu_sc0007023.1_g000007 Rmu_sc0007084.1_g000004 Rmu_sc0007239.1_g000001 Rmu_sc0007338.1_g000010 Rmu_sc0007398.1_g000004 Rmu_sc0007630.1_g000001 Rmu_sc0008067.1_g000001 Rmu_sc0008114.1_g000001 Rmu_sc0008623.1_g000008 Rmu_sc0009777.1_g000010 Rmu_sc0012719.1_g000001 Rmu_sc0014173.1_g000001 Rmu_sc0015338.1_g000002 Rmu_sc0015804.1_g000002 Rmu_sc0023779.1_g000001 Rmu_sc0024140.1_g000001 Rmu_sc0024833.1_g000006 Rmu_ssc0000087.1_g000013 Rmu_ssc0000400.1_g000088 Rmu_ssc0000400.1_g000089
rosa_roxburghii Rroxscaffold_175G00432540 Rroxscaffold_1G00003260 Rroxscaffold_1G00032490 Rroxscaffold_1G00035100 Rroxscaffold_1G00051960 Rroxscaffold_1G00056120 Rroxscaffold_2G00091260 Rroxscaffold_2G00097580 Rroxscaffold_2G00100910 Rroxscaffold_2G00101000 Rroxscaffold_2G00104370 Rroxscaffold_2G00154020 Rroxscaffold_3G00219010 Rroxscaffold_3G00241360 Rroxscaffold_3G00265190 Rroxscaffold_4G00328620 Rroxscaffold_5G00338660 Rroxscaffold_5G00339430 Rroxscaffold_5G00346490 Rroxscaffold_5G00360740 Rroxscaffold_5G00373490 Rroxscaffold_5G00373950 Rroxscaffold_5G00379290 Rroxscaffold_6G00390000 Rroxscaffold_6G00406560 Rroxscaffold_7G00163660 Rroxscaffold_7G00172220 Rroxscaffold_7G00182760 Rroxscaffold_7G00217680 Rroxscaffold_7G00217910
rosa_rugosa Rorug03G0299400 Rorug04G0048800 Rorug05G0503600 Rorug07G0090800 Rorug07G0090900 Rorug07G0294700
rosa_samantha Rh3BG243300 Rh4BG398100
rosa_wichuraiana Rw0G005950 Rw0G023070 Rw1G003680 Rw1G004130 Rw1G007910 Rw1G013940 Rw1G018710 Rw1G022140 Rw1G031170 Rw1G041420 Rw2G024950 Rw2G045000 Rw2G049360 Rw3G007370 Rw3G009120 Rw3G011780 Rw3G027830 Rw4G001510 Rw4G003110 Rw4G008370 Rw4G022100 Rw4G035490 Rw5G001650 Rw5G002000 Rw5G002590 Rw5G017930 Rw5G020530 Rw5G024010 Rw5G024710 Rw5G025620 Rw5G027980 Rw5G030200 Rw5G036530 Rw5G044700 Rw6G006860 Rw6G018940 Rw6G019810 Rw6G029740 Rw7G009360 Rw7G017280 Rw7G017540 Rw7G020090 Rw7G022500 Rw7G031420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 711, 714
AclWI GGATC 3 cut(s) 174, 479, 678
AcoI YGGCCR 3 cut(s) 591, 702, 722
AcuI CTGAAG 1 cut(s) 141
AfaI GTAC 2 cut(s) 211, 353
AfiI CCNNNNNNNGG 1 cut(s) 677
AflIII ACRYGT 1 cut(s) 603
AgsI TTSAA 6 cut(s) 10, 130, 349, 550, 579, 619
AluBI AGCT 7 cut(s) 47, 202, 256, 320, 421, 467, 584
AluI AGCT 7 cut(s) 47, 202, 256, 320, 421, 467, 584
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 1 cut(s) 722
AlwI GGATC 3 cut(s) 174, 479, 678
AoxI GGCC 3 cut(s) 591, 702, 722
ApeKI GCWGC 1 cut(s) 317
AseI ATTAAT 1 cut(s) 396
Asp700I GAANNNNTTC 1 cut(s) 545
AsuC2I CCSGG 1 cut(s) 678
AsuNHI GCTAGC 1 cut(s) 256
BalI TGGCCA 1 cut(s) 593
BbsI GAAGAC 1 cut(s) 660
Bbv12I GWGCWC 1 cut(s) 143
BbvI GCAGC 1 cut(s) 329
BccI CCATC 1 cut(s) 421
BceAI ACGGC 2 cut(s) 421, 709
BcnI CCSGG 1 cut(s) 678
BcoDI GTCTC 1 cut(s) 722
BfaI CTAG 4 cut(s) 53, 257, 321, 468
BfmI CTRYAG 4 cut(s) 40, 60, 495, 585
BisI GCNGC 2 cut(s) 318, 712
BlsI GCNGC 2 cut(s) 319, 713
Bme1390I CCNGG 1 cut(s) 678
BmiI GGNNCC 1 cut(s) 34
BmrFI CCNGG 1 cut(s) 678
BmsI GCATC 2 cut(s) 12, 269
BmtI GCTAGC 1 cut(s) 260
BpiI GAAGAC 1 cut(s) 660
Bpu10I CCTNAGC 1 cut(s) 252
BpuMI CCSGG 1 cut(s) 678
BsaI GGTCTC 1 cut(s) 722
BsaJI CCNNGG 2 cut(s) 330, 594
Bsc4I CCNNNNNNNGG 1 cut(s) 677
Bse1I ACTGG 1 cut(s) 34
BseDI CCNNGG 2 cut(s) 330, 594
BseGI GGATG 1 cut(s) 463
BseLI CCNNNNNNNGG 1 cut(s) 677
BseMII CTCAG 3 cut(s) 99, 243, 427
BseNI ACTGG 1 cut(s) 34
BseX3I CGGCCG 2 cut(s) 702, 722
BseXI GCAGC 1 cut(s) 329
Bsh1285I CGRYCG 2 cut(s) 705, 725
BshFI GGCC 3 cut(s) 593, 704, 724
BsiEI CGRYCG 2 cut(s) 705, 725
BsiHKAI GWGCWC 1 cut(s) 143
BsiSI CCGG 3 cut(s) 678, 701, 721
BslI CCNNNNNNNGG 1 cut(s) 677
BsmAI GTCTC 1 cut(s) 722
BsmI GAATGC 1 cut(s) 84
BsnI GGCC 3 cut(s) 593, 704, 724
Bso31I GGTCTC 1 cut(s) 722
Bsp1286I GDGCHC 1 cut(s) 143
Bsp143I GATC 4 cut(s) 179, 381, 471, 683
BspACI CCGC 2 cut(s) 711, 714
BspANI GGCC 3 cut(s) 593, 704, 724
BspCNI CTCAG 3 cut(s) 100, 244, 426
BspHI TCATGA 2 cut(s) 340, 384
BspLI GGNNCC 1 cut(s) 34
BspOI GCTAGC 1 cut(s) 260
BspPI GGATC 3 cut(s) 174, 479, 678
BspQI GCTCTTC 1 cut(s) 240
BspTNI GGTCTC 1 cut(s) 722
BsrI ACTGG 1 cut(s) 34
BssECI CCNNGG 2 cut(s) 330, 594
BssMI GATC 4 cut(s) 179, 381, 471, 683
BssT1I CCWWGG 2 cut(s) 330, 594
Bst4CI ACNGT 3 cut(s) 14, 229, 589
Bst6I CTCTTC 2 cut(s) 117, 240
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 6 cut(s) 108, 195, 252, 288, 413, 570
BstF5I GGATG 1 cut(s) 463
BstKTI GATC 4 cut(s) 182, 384, 474, 686
BstMAI GTCTC 1 cut(s) 722
BstMBI GATC 4 cut(s) 179, 381, 471, 683
BstMCI CGRYCG 2 cut(s) 705, 725
BstMWI GCNNNNNNNGC 2 cut(s) 590, 630
BstNSI RCATGY 1 cut(s) 607
BstSCI CCNGG 1 cut(s) 676
BstSFI CTRYAG 4 cut(s) 40, 60, 495, 585
BstV1I GCAGC 1 cut(s) 329
BstV2I GAAGAC 1 cut(s) 660
BstX2I RGATCY 2 cut(s) 179, 683
BstXI CCANNNNNNTGG 1 cut(s) 190
BstYI RGATCY 2 cut(s) 179, 683
BstZI CGGCCG 2 cut(s) 702, 722
BsuRI GGCC 3 cut(s) 593, 704, 724
BtsCI GGATG 1 cut(s) 463
BtsIMutI CAGTG 2 cut(s) 19, 594
Cac8I GCNNGC 1 cut(s) 258
CciI TCATGA 2 cut(s) 340, 384
Csp6I GTAC 2 cut(s) 210, 352
CviAII CATG 5 cut(s) 83, 341, 355, 385, 604
CviQI GTAC 2 cut(s) 210, 352
DdeI CTNAG 6 cut(s) 108, 195, 252, 288, 413, 570
DpnI GATC 4 cut(s) 181, 383, 473, 685
DpnII GATC 4 cut(s) 179, 381, 471, 683
EaeI YGGCCR 3 cut(s) 591, 702, 722
EagI CGGCCG 2 cut(s) 702, 722
Eam1104I CTCTTC 2 cut(s) 117, 240
EarI CTCTTC 2 cut(s) 117, 240
EclXI CGGCCG 2 cut(s) 702, 722
Eco130I CCWWGG 2 cut(s) 330, 594
Eco31I GGTCTC 1 cut(s) 722
Eco52I CGGCCG 2 cut(s) 702, 722
Eco57I CTGAAG 1 cut(s) 141
EcoT14I CCWWGG 2 cut(s) 330, 594
EcoT22I ATGCAT 1 cut(s) 84
ErhI CCWWGG 2 cut(s) 330, 594
FaeI CATG 5 cut(s) 86, 344, 358, 388, 607
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FatI CATG 5 cut(s) 82, 340, 354, 384, 603
Fnu4HI GCNGC 2 cut(s) 318, 712
FokI GGATG 1 cut(s) 470
Fsp4HI GCNGC 2 cut(s) 318, 712
FspBI CTAG 4 cut(s) 53, 257, 321, 468
GluI GCNGC 2 cut(s) 318, 712
HaeIII GGCC 3 cut(s) 593, 704, 724
HapII CCGG 3 cut(s) 678, 701, 721
Hin1II CATG 5 cut(s) 86, 344, 358, 388, 607
HinfI GANTC 2 cut(s) 56, 411
HpaII CCGG 3 cut(s) 678, 701, 721
Hpy188I TCNGA 2 cut(s) 265, 688
Hpy188III TCNNGA 4 cut(s) 19, 272, 341, 385
HpyCH4III ACNGT 3 cut(s) 14, 229, 589
HpyCH4V TGCA 2 cut(s) 82, 633
HpyF10VI GCNNNNNNNGC 2 cut(s) 590, 630
HpyF3I CTNAG 6 cut(s) 108, 195, 252, 288, 413, 570
Hsp92II CATG 5 cut(s) 86, 344, 358, 388, 607
Kzo9I GATC 4 cut(s) 179, 381, 471, 683
LguI GCTCTTC 1 cut(s) 240
LmnI GCTCC 1 cut(s) 418
Lsp1109I GCAGC 1 cut(s) 329
LweI GCATC 2 cut(s) 12, 269
MaeI CTAG 4 cut(s) 53, 257, 321, 468
MalI GATC 4 cut(s) 181, 383, 473, 685
MboI GATC 4 cut(s) 179, 381, 471, 683
MboII GAAGA 5 cut(s) 134, 257, 400, 662, 665
MflI RGATCY 2 cut(s) 179, 683
MhlI GDGCHC 1 cut(s) 143
MlsI TGGCCA 1 cut(s) 593
MluNI TGGCCA 1 cut(s) 593
MlyI GAGTC 1 cut(s) 405
MmeI TCCRAC 1 cut(s) 711
MnlI CCTC 4 cut(s) 103, 223, 454, 518
Mox20I TGGCCA 1 cut(s) 593
Mph1103I ATGCAT 1 cut(s) 84
MroXI GAANNNNTTC 1 cut(s) 545
MscI TGGCCA 1 cut(s) 593
MseI TTAA 1 cut(s) 396
MslI CAYNNNNRTG 2 cut(s) 389, 452
Msp20I TGGCCA 1 cut(s) 593
MspI CCGG 3 cut(s) 678, 701, 721
MspR9I CCNGG 1 cut(s) 678
Mva1269I GAATGC 1 cut(s) 84
MwoI GCNNNNNNNGC 2 cut(s) 590, 630
NciI CCSGG 1 cut(s) 678
NdeII GATC 4 cut(s) 179, 381, 471, 683
NheI GCTAGC 1 cut(s) 256
NlaIII CATG 5 cut(s) 86, 344, 358, 388, 607
NlaIV GGNNCC 1 cut(s) 34
NsiI ATGCAT 1 cut(s) 84
NspI RCATGY 1 cut(s) 607
PagI TCATGA 2 cut(s) 340, 384
PciI ACATGT 1 cut(s) 603
PciSI GCTCTTC 1 cut(s) 240
PctI GAATGC 1 cut(s) 84
PdmI GAANNNNTTC 1 cut(s) 545
PfeI GAWTC 1 cut(s) 56
PkrI GCNGC 2 cut(s) 319, 713
PleI GAGTC 1 cut(s) 405
PpsI GAGTC 1 cut(s) 405
PscI ACATGT 1 cut(s) 603
PshBI ATTAAT 1 cut(s) 396
PspN4I GGNNCC 1 cut(s) 34
PsuI RGATCY 2 cut(s) 179, 683
RsaI GTAC 2 cut(s) 211, 353
RsaNI GTAC 2 cut(s) 210, 352
RseI CAYNNNNRTG 2 cut(s) 389, 452
SapI GCTCTTC 1 cut(s) 240
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 2 cut(s) 318, 712
Sau3AI GATC 4 cut(s) 179, 381, 471, 683
SchI GAGTC 1 cut(s) 405
ScrFI CCNGG 1 cut(s) 678
SduI GDGCHC 1 cut(s) 143
SfaNI GCATC 2 cut(s) 12, 269
SfcI CTRYAG 4 cut(s) 40, 60, 495, 585
SmiMI CAYNNNNRTG 2 cut(s) 389, 452
SsiI CCGC 2 cut(s) 711, 714
SspMI CTAG 4 cut(s) 53, 257, 321, 468
StyD4I CCNGG 1 cut(s) 676
StyI CCWWGG 2 cut(s) 330, 594
TaaI ACNGT 3 cut(s) 14, 229, 589
TaqI TCGA 1 cut(s) 648
TaqII GACCGA 1 cut(s) 672
TatI WGTACW 1 cut(s) 351
TauI GCSGC 1 cut(s) 714
TfiI GAWTC 1 cut(s) 56
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TscAI CASTG 2 cut(s) 19, 594
TseI GCWGC 1 cut(s) 317
TspDTI ATGAA 4 cut(s) 17, 147, 329, 401
TspRI CASTG 2 cut(s) 19, 594
VspI ATTAAT 1 cut(s) 396
XceI RCATGY 1 cut(s) 607
XmnI GAANNNNTTC 1 cut(s) 545
XspI CTAG 4 cut(s) 53, 257, 321, 468
Zsp2I ATGCAT 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.