Domain of unknown function (DUF4413)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1202 |
| Acc65I | GGTACC | 1 cut(s) | 2073 |
| AccB1I | GGYRCC | 1 cut(s) | 2073 |
| AccI | GTMKAC | 1 cut(s) | 128 |
| AciI | CCGC | 1 cut(s) | 322 |
| AcsI | RAATTY | 7 cut(s) | 802, 979, 1204, 1314, 1773, 1946, 2199 |
| AcuI | CTGAAG | 1 cut(s) | 746 |
| AcvI | CACGTG | 1 cut(s) | 2099 |
| AfaI | GTAC | 4 cut(s) | 80, 393, 1906, 2075 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 46, 120, 191, 1146, 1157 |
| AflIII | ACRYGT | 3 cut(s) | 14, 1195, 1273 |
| AgeI | ACCGGT | 1 cut(s) | 55 |
| AjnI | CCWGG | 2 cut(s) | 1885, 1919 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1208, 1240 |
| AleI | CACNNNNGTG | 1 cut(s) | 261 |
| AluBI | AGCT | 7 cut(s) | 93, 1406, 1497, 1831, 1880, 1896, 2184 |
| AluI | AGCT | 7 cut(s) | 93, 1406, 1497, 1831, 1880, 1896, 2184 |
| Alw26I | GTCTC | 4 cut(s) | 184, 562, 1816, 1952 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2141 |
| Ama87I | CYCGRG | 1 cut(s) | 45 |
| AoxI | GGCC | 2 cut(s) | 1148, 1293 |
| ApeKI | GCWGC | 3 cut(s) | 503, 1190, 1893 |
| ApoI | RAATTY | 7 cut(s) | 802, 979, 1204, 1314, 1773, 1946, 2199 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 735, 767 |
| AseI | ATTAAT | 2 cut(s) | 1610, 2213 |
| AsiGI | ACCGGT | 1 cut(s) | 55 |
| Asp718I | GGTACC | 1 cut(s) | 2073 |
| AspS9I | GGNCC | 6 cut(s) | 27, 49, 432, 664, 1294, 2059 |
| AsuC2I | CCSGG | 2 cut(s) | 46, 47 |
| AsuHPI | GGTGA | 3 cut(s) | 193, 566, 1255 |
| AvaI | CYCGRG | 1 cut(s) | 45 |
| AvaII | GGWCC | 5 cut(s) | 27, 49, 432, 664, 2059 |
| AxyI | CCTNAGG | 2 cut(s) | 443, 1643 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2057, 2090 |
| BamHI | GGATCC | 3 cut(s) | 36, 140, 1729 |
| BanI | GGYRCC | 1 cut(s) | 2073 |
| BanII | GRGCYC | 1 cut(s) | 1631 |
| BbrPI | CACGTG | 1 cut(s) | 2099 |
| BbvI | GCAGC | 3 cut(s) | 515, 1177, 1905 |
| BccI | CCATC | 9 cut(s) | 212, 277, 314, 1039, 1368, 1811, 2086, 2150, 2233 |
| BceAI | ACGGC | 1 cut(s) | 1703 |
| BciT130I | CCWGG | 2 cut(s) | 1887, 1921 |
| BciVI | GTATCC | 1 cut(s) | 1569 |
| BclI | TGATCA | 3 cut(s) | 607, 658, 1867 |
| BcnI | CCSGG | 2 cut(s) | 46, 47 |
| BcoDI | GTCTC | 4 cut(s) | 184, 562, 1816, 1952 |
| BfaI | CTAG | 3 cut(s) | 191, 1902, 2108 |
| BfmI | CTRYAG | 3 cut(s) | 820, 962, 2003 |
| BfuI | GTATCC | 1 cut(s) | 1569 |
| BglII | AGATCT | 1 cut(s) | 1134 |
| BisI | GCNGC | 3 cut(s) | 504, 1191, 1894 |
| BlsI | GCNGC | 3 cut(s) | 505, 1192, 1895 |
| Bme1390I | CCNGG | 4 cut(s) | 46, 47, 1887, 1921 |
| Bme18I | GGWCC | 5 cut(s) | 27, 49, 432, 664, 2059 |
| BmeT110I | CYCGRG | 1 cut(s) | 45 |
| BmgT120I | GGNCC | 6 cut(s) | 27, 49, 432, 664, 1294, 2059 |
| BmiI | GGNNCC | 7 cut(s) | 38, 50, 142, 433, 1731, 2010, 2075 |
| BmrFI | CCNGG | 4 cut(s) | 46, 47, 1887, 1921 |
| BmrI | ACTGGG | 2 cut(s) | 1060, 1361 |
| BmsI | GCATC | 4 cut(s) | 92, 1210, 1828, 1830 |
| BmuI | ACTGGG | 2 cut(s) | 1060, 1361 |
| BoxI | GACNNNNGTC | 1 cut(s) | 669 |
| BpmI | CTGGAG | 1 cut(s) | 888 |
| BpuMI | CCSGG | 2 cut(s) | 46, 47 |
| Bsa29I | ATCGAT | 1 cut(s) | 2260 |
| BsaAI | YACGTR | 2 cut(s) | 15, 2099 |
| BsaBI | GATNNNNATC | 2 cut(s) | 612, 2256 |
| BsaJI | CCNNGG | 4 cut(s) | 45, 1919, 1920, 2069 |
| BsaWI | WCCGGW | 1 cut(s) | 55 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1121, 1151 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 46, 120, 191, 1146, 1157 |
| Bse118I | RCCGGY | 1 cut(s) | 55 |
| Bse1I | ACTGG | 2 cut(s) | 1055, 1367 |
| Bse21I | CCTNAGG | 2 cut(s) | 443, 1643 |
| Bse3DI | GCAATG | 1 cut(s) | 713 |
| Bse8I | GATNNNNATC | 2 cut(s) | 612, 2256 |
| BseBI | CCWGG | 2 cut(s) | 1887, 1921 |
| BseCI | ATCGAT | 1 cut(s) | 2260 |
| BseDI | CCNNGG | 4 cut(s) | 45, 1919, 1920, 2069 |
| BseGI | GGATG | 4 cut(s) | 10, 204, 631, 1813 |
| BseJI | GATNNNNATC | 2 cut(s) | 612, 2256 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 46, 120, 191, 1146, 1157 |
| BseMI | GCAATG | 1 cut(s) | 713 |
| BseMII | CTCAG | 6 cut(s) | 424, 434, 1226, 1575, 1657, 1979 |
| BseNI | ACTGG | 2 cut(s) | 1055, 1367 |
| BseRI | GAGGAG | 2 cut(s) | 965, 1360 |
| BseXI | GCAGC | 3 cut(s) | 515, 1177, 1905 |
| BseYI | CCCAGC | 1 cut(s) | 1320 |
| BsgI | GTGCAG | 3 cut(s) | 918, 1176, 1717 |
| BshFI | GGCC | 2 cut(s) | 1150, 1295 |
| BshNI | GGYRCC | 1 cut(s) | 2073 |
| BshTI | ACCGGT | 1 cut(s) | 55 |
| BshVI | ATCGAT | 1 cut(s) | 2260 |
| BsiHKCI | CYCGRG | 1 cut(s) | 45 |
| BsiSI | CCGG | 2 cut(s) | 46, 56 |
| BslFI | GGGAC | 1 cut(s) | 445 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 46, 120, 191, 1146, 1157 |
| BsmAI | GTCTC | 4 cut(s) | 184, 562, 1816, 1952 |
| BsmFI | GGGAC | 1 cut(s) | 445 |
| BsmI | GAATGC | 1 cut(s) | 2144 |
| BsnI | GGCC | 2 cut(s) | 1150, 1295 |
| BsoBI | CYCGRG | 1 cut(s) | 45 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1631 |
| Bsp1407I | TGTACA | 1 cut(s) | 391 |
| BspACI | CCGC | 1 cut(s) | 322 |
| BspANI | GGCC | 2 cut(s) | 1150, 1295 |
| BspCNI | CTCAG | 6 cut(s) | 423, 435, 1227, 1576, 1656, 1978 |
| BspDI | ATCGAT | 1 cut(s) | 2260 |
| BspLI | GGNNCC | 7 cut(s) | 38, 50, 142, 433, 1731, 2010, 2075 |
| BspMAI | CTGCAG | 1 cut(s) | 966 |
| BspT107I | GGYRCC | 1 cut(s) | 2073 |
| BsrDI | GCAATG | 1 cut(s) | 713 |
| BsrFI | RCCGGY | 1 cut(s) | 55 |
| BsrGI | TGTACA | 1 cut(s) | 391 |
| BsrI | ACTGG | 2 cut(s) | 1055, 1367 |
| BssAI | RCCGGY | 1 cut(s) | 55 |
| BssECI | CCNNGG | 4 cut(s) | 45, 1919, 1920, 2069 |
| BssNAI | GTATAC | 1 cut(s) | 129 |
| BssT1I | CCWWGG | 1 cut(s) | 2069 |
| Bst1107I | GTATAC | 1 cut(s) | 129 |
| Bst2UI | CCWGG | 2 cut(s) | 1887, 1921 |
| Bst4CI | ACNGT | 5 cut(s) | 398, 532, 668, 1064, 1340 |
| Bst6I | CTCTTC | 1 cut(s) | 503 |
| BstAUI | TGTACA | 1 cut(s) | 391 |
| BstBAI | YACGTR | 2 cut(s) | 15, 2099 |
| BstC8I | GCNNGC | 4 cut(s) | 688, 1898, 2186, 2222 |
| BstDEI | CTNAG | 8 cut(s) | 94, 410, 443, 1118, 1235, 1584, 1643, 1965 |
| BstEII | GGTNACC | 1 cut(s) | 707 |
| BstF5I | GGATG | 4 cut(s) | 10, 204, 631, 1813 |
| BstMAI | GTCTC | 4 cut(s) | 184, 562, 1816, 1952 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1229 |
| BstNI | CCWGG | 2 cut(s) | 1887, 1921 |
| BstNSI | RCATGY | 3 cut(s) | 1199, 1277, 1389 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 669 |
| BstPI | GGTNACC | 1 cut(s) | 707 |
| BstSCI | CCNGG | 4 cut(s) | 44, 45, 1885, 1919 |
| BstSFI | CTRYAG | 3 cut(s) | 820, 962, 2003 |
| BstV1I | GCAGC | 3 cut(s) | 515, 1177, 1905 |
| BstX2I | RGATCY | 5 cut(s) | 36, 140, 194, 1134, 1729 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 263, 1328, 1374 |
| BstYI | RGATCY | 5 cut(s) | 36, 140, 194, 1134, 1729 |
| BstZ17I | GTATAC | 1 cut(s) | 129 |
| Bsu15I | ATCGAT | 1 cut(s) | 2260 |
| Bsu36I | CCTNAGG | 2 cut(s) | 443, 1643 |
| BsuI | GTATCC | 1 cut(s) | 1569 |
| BsuRI | GGCC | 2 cut(s) | 1150, 1295 |
| BsuTUI | ATCGAT | 1 cut(s) | 2260 |
| BtgZI | GCGATG | 1 cut(s) | 475 |
| BtsCI | GGATG | 4 cut(s) | 10, 204, 631, 1813 |
| BtsI | GCAGTG | 1 cut(s) | 1918 |
| BtsIMutI | CAGTG | 6 cut(s) | 394, 418, 1098, 1232, 1345, 1918 |
| Cac8I | GCNNGC | 4 cut(s) | 688, 1898, 2186, 2222 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2141 |
| Cfr10I | RCCGGY | 1 cut(s) | 55 |
| Cfr13I | GGNCC | 6 cut(s) | 27, 49, 432, 664, 1294, 2059 |
| Cfr9I | CCCGGG | 1 cut(s) | 45 |
| ClaI | ATCGAT | 1 cut(s) | 2260 |
| Csp6I | GTAC | 4 cut(s) | 79, 392, 1905, 2074 |
| CspAI | ACCGGT | 1 cut(s) | 55 |
| CviQI | GTAC | 4 cut(s) | 79, 392, 1905, 2074 |
| DdeI | CTNAG | 8 cut(s) | 94, 410, 443, 1118, 1235, 1584, 1643, 1965 |
| Eam1104I | CTCTTC | 1 cut(s) | 503 |
| EarI | CTCTTC | 1 cut(s) | 503 |
| Eco130I | CCWWGG | 1 cut(s) | 2069 |
| Eco147I | AGGCCT | 1 cut(s) | 1150 |
| Eco24I | GRGCYC | 1 cut(s) | 1631 |
| Eco32I | GATATC | 1 cut(s) | 817 |
| Eco47I | GGWCC | 5 cut(s) | 27, 49, 432, 664, 2059 |
| Eco57I | CTGAAG | 1 cut(s) | 746 |
| Eco72I | CACGTG | 1 cut(s) | 2099 |
| Eco81I | CCTNAGG | 2 cut(s) | 443, 1643 |
| Eco88I | CYCGRG | 1 cut(s) | 45 |
| Eco91I | GGTNACC | 1 cut(s) | 707 |
| EcoO65I | GGTNACC | 1 cut(s) | 707 |
| EcoRII | CCWGG | 2 cut(s) | 1885, 1919 |
| EcoRV | GATATC | 1 cut(s) | 817 |
| EcoT14I | CCWWGG | 1 cut(s) | 2069 |
| EcoT22I | ATGCAT | 2 cut(s) | 1543, 1843 |
| EcoT38I | GRGCYC | 1 cut(s) | 1631 |
| ErhI | CCWWGG | 1 cut(s) | 2069 |
| FaqI | GGGAC | 1 cut(s) | 445 |
| FbaI | TGATCA | 3 cut(s) | 607, 658, 1867 |
| FblI | GTMKAC | 1 cut(s) | 128 |
| Fnu4HI | GCNGC | 3 cut(s) | 504, 1191, 1894 |
| FokI | GGATG | 4 cut(s) | 17, 191, 638, 1820 |
| FriOI | GRGCYC | 1 cut(s) | 1631 |
| Fsp4HI | GCNGC | 3 cut(s) | 504, 1191, 1894 |
| FspBI | CTAG | 3 cut(s) | 191, 1902, 2108 |
| GluI | GCNGC | 3 cut(s) | 504, 1191, 1894 |
| GsaI | CCCAGC | 1 cut(s) | 1324 |
| GsuI | CTGGAG | 1 cut(s) | 888 |
| HaeIII | GGCC | 2 cut(s) | 1150, 1295 |
| HapII | CCGG | 2 cut(s) | 46, 56 |
| HincII | GTYRAC | 2 cut(s) | 327, 2124 |
| HindII | GTYRAC | 2 cut(s) | 327, 2124 |
| HindIII | AAGCTT | 2 cut(s) | 91, 1495 |
| HpaII | CCGG | 2 cut(s) | 46, 56 |
| HphI | GGTGA | 3 cut(s) | 193, 566, 1255 |
| Hpy166II | GTNNAC | 8 cut(s) | 129, 327, 392, 394, 1286, 1600, 2062, 2124 |
| Hpy8I | GTNNAC | 8 cut(s) | 129, 327, 392, 394, 1286, 1600, 2062, 2124 |
| HpyAV | CCTTC | 6 cut(s) | 358, 507, 770, 902, 1927, 2050 |
| HpyCH4III | ACNGT | 5 cut(s) | 398, 532, 668, 1064, 1340 |
| HpyCH4IV | ACGT | 2 cut(s) | 14, 2098 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1229 |
| HpyF3I | CTNAG | 8 cut(s) | 94, 410, 443, 1118, 1235, 1584, 1643, 1965 |
| HpySE526I | ACGT | 2 cut(s) | 14, 2098 |
| KpnI | GGTACC | 1 cut(s) | 2077 |
| Ksp22I | TGATCA | 3 cut(s) | 607, 658, 1867 |
| LmnI | GCTCC | 3 cut(s) | 1689, 1828, 2008 |
| Lsp1109I | GCAGC | 3 cut(s) | 515, 1177, 1905 |
| LweI | GCATC | 4 cut(s) | 92, 1210, 1828, 1830 |
| MaeI | CTAG | 3 cut(s) | 191, 1902, 2108 |
| MaeII | ACGT | 2 cut(s) | 14, 2098 |
| MaeIII | GTNAC | 5 cut(s) | 398, 634, 707, 985, 1978 |
| MboII | GAAGA | 3 cut(s) | 520, 979, 1810 |
| MfeI | CAATTG | 1 cut(s) | 2283 |
| MflI | RGATCY | 5 cut(s) | 36, 140, 194, 1134, 1729 |
| MhlI | GDGCHC | 1 cut(s) | 1631 |
| MlyI | GAGTC | 1 cut(s) | 1978 |
| MmeI | TCCRAC | 3 cut(s) | 76, 1206, 1586 |
| Mph1103I | ATGCAT | 2 cut(s) | 1543, 1843 |
| MslI | CAYNNNNRTG | 5 cut(s) | 15, 261, 276, 1008, 2215 |
| MspI | CCGG | 2 cut(s) | 46, 56 |
| MspR9I | CCNGG | 4 cut(s) | 46, 47, 1887, 1921 |
| MunI | CAATTG | 1 cut(s) | 2283 |
| Mva1269I | GAATGC | 1 cut(s) | 2144 |
| MvaI | CCWGG | 2 cut(s) | 1887, 1921 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1229 |
| NciI | CCSGG | 2 cut(s) | 46, 47 |
| NlaIV | GGNNCC | 7 cut(s) | 38, 50, 142, 433, 1731, 2010, 2075 |
| NmuCI | GTSAC | 2 cut(s) | 985, 1978 |
| NsiI | ATGCAT | 2 cut(s) | 1543, 1843 |
| NspI | RCATGY | 3 cut(s) | 1199, 1277, 1389 |
| OliI | CACNNNNGTG | 1 cut(s) | 261 |
| PasI | CCCWGGG | 1 cut(s) | 1920 |
| PceI | AGGCCT | 1 cut(s) | 1150 |
| PciI | ACATGT | 2 cut(s) | 1195, 1273 |
| PctI | GAATGC | 1 cut(s) | 2144 |
| PinAI | ACCGGT | 1 cut(s) | 55 |
| PkrI | GCNGC | 3 cut(s) | 505, 1192, 1895 |
| PleI | GAGTC | 1 cut(s) | 1977 |
| PmaCI | CACGTG | 1 cut(s) | 2099 |
| PmlI | CACGTG | 1 cut(s) | 2099 |
| PpsI | GAGTC | 1 cut(s) | 1977 |
| Ppu21I | YACGTR | 2 cut(s) | 15, 2099 |
| PscI | ACATGT | 2 cut(s) | 1195, 1273 |
| PshAI | GACNNNNGTC | 1 cut(s) | 669 |
| PshBI | ATTAAT | 2 cut(s) | 1610, 2213 |
| PsiI | TTATAA | 1 cut(s) | 1202 |
| Psp6I | CCWGG | 2 cut(s) | 1885, 1919 |
| PspCI | CACGTG | 1 cut(s) | 2099 |
| PspEI | GGTNACC | 1 cut(s) | 707 |
| PspFI | CCCAGC | 1 cut(s) | 1320 |
| PspGI | CCWGG | 2 cut(s) | 1885, 1919 |
| PspN4I | GGNNCC | 7 cut(s) | 38, 50, 142, 433, 1731, 2010, 2075 |
| PspPI | GGNCC | 6 cut(s) | 27, 49, 432, 664, 1294, 2059 |
| PstI | CTGCAG | 1 cut(s) | 966 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2141 |
| PsuI | RGATCY | 5 cut(s) | 36, 140, 194, 1134, 1729 |
| RsaI | GTAC | 4 cut(s) | 80, 393, 1906, 2075 |
| RsaNI | GTAC | 4 cut(s) | 79, 392, 1905, 2074 |
| RseI | CAYNNNNRTG | 5 cut(s) | 15, 261, 276, 1008, 2215 |
| SatI | GCNGC | 3 cut(s) | 504, 1191, 1894 |
| Sau96I | GGNCC | 6 cut(s) | 27, 49, 432, 664, 1294, 2059 |
| SchI | GAGTC | 1 cut(s) | 1978 |
| ScrFI | CCNGG | 4 cut(s) | 46, 47, 1887, 1921 |
| SduI | GDGCHC | 1 cut(s) | 1631 |
| SfaNI | GCATC | 4 cut(s) | 92, 1210, 1828, 1830 |
| SfcI | CTRYAG | 3 cut(s) | 820, 962, 2003 |
| SinI | GGWCC | 5 cut(s) | 27, 49, 432, 664, 2059 |
| SmaI | CCCGGG | 1 cut(s) | 47 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 15, 261, 276, 1008, 2215 |
| SseBI | AGGCCT | 1 cut(s) | 1150 |
| SsiI | CCGC | 1 cut(s) | 322 |
| SspI | AATATT | 1 cut(s) | 1685 |
| SspMI | CTAG | 3 cut(s) | 191, 1902, 2108 |
| StuI | AGGCCT | 1 cut(s) | 1150 |
| StyD4I | CCNGG | 4 cut(s) | 44, 45, 1885, 1919 |
| StyI | CCWWGG | 1 cut(s) | 2069 |
| TaaI | ACNGT | 5 cut(s) | 398, 532, 668, 1064, 1340 |
| TaiI | ACGT | 2 cut(s) | 17, 2101 |
| TaqI | TCGA | 8 cut(s) | 798, 1208, 1258, 1372, 2179, 2203, 2260, 2267 |
| TatI | WGTACW | 1 cut(s) | 391 |
| TscAI | CASTG | 6 cut(s) | 401, 418, 1105, 1239, 1345, 1918 |
| TseFI | GTSAC | 2 cut(s) | 985, 1978 |
| TseI | GCWGC | 3 cut(s) | 503, 1190, 1893 |
| Tsp45I | GTSAC | 2 cut(s) | 985, 1978 |
| TspGWI | ACGGA | 1 cut(s) | 40 |
| TspMI | CCCGGG | 1 cut(s) | 45 |
| TspRI | CASTG | 6 cut(s) | 401, 418, 1105, 1239, 1345, 1918 |
| VpaK11BI | GGWCC | 5 cut(s) | 27, 49, 432, 664, 2059 |
| VspI | ATTAAT | 2 cut(s) | 1610, 2213 |
| XapI | RAATTY | 7 cut(s) | 802, 979, 1204, 1314, 1773, 1946, 2199 |
| XceI | RCATGY | 3 cut(s) | 1199, 1277, 1389 |
| XmaI | CCCGGG | 1 cut(s) | 45 |
| XmiI | GTMKAC | 1 cut(s) | 128 |
| XspI | CTAG | 3 cut(s) | 191, 1902, 2108 |
| Zsp2I | ATGCAT | 2 cut(s) | 1543, 1843 |