BED zinc finger
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 431 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 564, 730 |
| Acc65I | GGTACC | 2 cut(s) | 37, 679 |
| AccB1I | GGYRCC | 2 cut(s) | 37, 679 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1084, 1950 |
| AccI | GTMKAC | 1 cut(s) | 1552 |
| AccII | CGCG | 1 cut(s) | 948 |
| AciI | CCGC | 1 cut(s) | 355 |
| AclI | AACGTT | 2 cut(s) | 473, 1227 |
| AclWI | GGATC | 4 cut(s) | 569, 1412, 1425, 1496 |
| AcoI | YGGCCR | 1 cut(s) | 501 |
| AcsI | RAATTY | 6 cut(s) | 228, 433, 646, 992, 1284, 1461 |
| AcuI | CTGAAG | 3 cut(s) | 957, 1025, 1629 |
| AdeI | CACNNNGTG | 1 cut(s) | 412 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 695, 1084, 1644, 1950 |
| AjnI | CCWGG | 4 cut(s) | 40, 274, 553, 600 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 786, 818 |
| AluBI | AGCT | 7 cut(s) | 336, 716, 1003, 1015, 1039, 1223, 1335 |
| AluI | AGCT | 7 cut(s) | 336, 716, 1003, 1015, 1039, 1223, 1335 |
| Alw21I | GWGCWC | 2 cut(s) | 99, 328 |
| Alw26I | GTCTC | 1 cut(s) | 1475 |
| AlwI | GGATC | 4 cut(s) | 569, 1412, 1425, 1496 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 601 |
| Ama87I | CYCGRG | 3 cut(s) | 1526, 1743, 1925 |
| AoxI | GGCC | 3 cut(s) | 120, 501, 751 |
| ApeKI | GCWGC | 7 cut(s) | 357, 360, 1220, 1307, 1386, 1613, 1916 |
| ApoI | RAATTY | 6 cut(s) | 228, 433, 646, 992, 1284, 1461 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1534, 1566 |
| AseI | ATTAAT | 1 cut(s) | 1565 |
| Asp718I | GGTACC | 2 cut(s) | 37, 679 |
| AspLEI | GCGC | 2 cut(s) | 69, 1750 |
| AspS9I | GGNCC | 4 cut(s) | 120, 352, 751, 1964 |
| AsuHPI | GGTGA | 3 cut(s) | 710, 754, 1441 |
| AvaI | CYCGRG | 3 cut(s) | 1526, 1743, 1925 |
| AvaII | GGWCC | 2 cut(s) | 352, 1964 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 285, 318 |
| BalI | TGGCCA | 1 cut(s) | 503 |
| BamHI | GGATCC | 1 cut(s) | 1417 |
| BanI | GGYRCC | 2 cut(s) | 37, 679 |
| BanII | GRGCYC | 1 cut(s) | 822 |
| BauI | CACGAG | 2 cut(s) | 392, 882 |
| BbsI | GAAGAC | 1 cut(s) | 974 |
| Bbv12I | GWGCWC | 2 cut(s) | 99, 328 |
| BbvI | GCAGC | 7 cut(s) | 344, 372, 1232, 1319, 1373, 1625, 1928 |
| BccI | CCATC | 6 cut(s) | 181, 512, 691, 773, 1499, 2014 |
| BceAI | ACGGC | 2 cut(s) | 228, 1295 |
| BciT130I | CCWGG | 4 cut(s) | 42, 276, 555, 602 |
| BciVI | GTATCC | 1 cut(s) | 408 |
| BclI | TGATCA | 2 cut(s) | 346, 1341 |
| BcoDI | GTCTC | 1 cut(s) | 1475 |
| BfaI | CTAG | 3 cut(s) | 132, 321, 1026 |
| BfmI | CTRYAG | 1 cut(s) | 358 |
| BfuI | GTATCC | 1 cut(s) | 408 |
| BglII | AGATCT | 2 cut(s) | 158, 1595 |
| BisI | GCNGC | 7 cut(s) | 358, 361, 1221, 1308, 1387, 1614, 1917 |
| BlsI | GCNGC | 7 cut(s) | 359, 362, 1222, 1309, 1388, 1615, 1918 |
| BmcAI | AGTACT | 1 cut(s) | 1208 |
| Bme1390I | CCNGG | 4 cut(s) | 42, 276, 555, 602 |
| Bme18I | GGWCC | 2 cut(s) | 352, 1964 |
| BmeT110I | CYCGRG | 3 cut(s) | 1526, 1743, 1925 |
| BmgT120I | GGNCC | 4 cut(s) | 120, 352, 751, 1964 |
| BmiI | GGNNCC | 6 cut(s) | 39, 121, 280, 681, 821, 1419 |
| BmrFI | CCNGG | 4 cut(s) | 42, 276, 555, 602 |
| BmrI | ACTGGG | 1 cut(s) | 230 |
| BmsI | GCATC | 5 cut(s) | 280, 1207, 1290, 1527, 1631 |
| BmuI | ACTGGG | 1 cut(s) | 230 |
| BpiI | GAAGAC | 1 cut(s) | 974 |
| Bsa29I | ATCGAT | 1 cut(s) | 1047 |
| BsaBI | GATNNNNATC | 2 cut(s) | 383, 1665 |
| BsaJI | CCNNGG | 4 cut(s) | 553, 601, 1421, 1944 |
| BsaWI | WCCGGW | 1 cut(s) | 1555 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 695, 1084, 1644, 1950 |
| Bse118I | RCCGGY | 1 cut(s) | 753 |
| Bse1I | ACTGG | 6 cut(s) | 148, 181, 236, 550, 647, 1838 |
| Bse8I | GATNNNNATC | 2 cut(s) | 383, 1665 |
| BseBI | CCWGG | 4 cut(s) | 42, 276, 555, 602 |
| BseCI | ATCGAT | 1 cut(s) | 1047 |
| BseDI | CCNNGG | 4 cut(s) | 553, 601, 1421, 1944 |
| BseGI | GGATG | 2 cut(s) | 131, 192 |
| BseJI | GATNNNNATC | 2 cut(s) | 383, 1665 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 695, 1084, 1644, 1950 |
| BseMII | CTCAG | 3 cut(s) | 470, 1227, 1470 |
| BseNI | ACTGG | 6 cut(s) | 148, 181, 236, 550, 647, 1838 |
| BseXI | GCAGC | 7 cut(s) | 344, 372, 1232, 1319, 1373, 1625, 1928 |
| BseYI | CCCAGC | 1 cut(s) | 913 |
| BsgI | GTGCAG | 1 cut(s) | 1935 |
| Bsh1236I | CGCG | 1 cut(s) | 948 |
| BshFI | GGCC | 3 cut(s) | 122, 503, 753 |
| BshNI | GGYRCC | 2 cut(s) | 37, 679 |
| BshVI | ATCGAT | 1 cut(s) | 1047 |
| BsiHKAI | GWGCWC | 2 cut(s) | 99, 328 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1526, 1743, 1925 |
| BsiSI | CCGG | 3 cut(s) | 123, 754, 1556 |
| BslFI | GGGAC | 1 cut(s) | 1079 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 695, 1084, 1644, 1950 |
| BsmAI | GTCTC | 1 cut(s) | 1475 |
| BsmFI | GGGAC | 1 cut(s) | 1079 |
| BsmI | GAATGC | 3 cut(s) | 500, 1480, 1548 |
| BsnI | GGCC | 3 cut(s) | 122, 503, 753 |
| BsoBI | CYCGRG | 3 cut(s) | 1526, 1743, 1925 |
| Bsp1286I | GDGCHC | 3 cut(s) | 99, 328, 822 |
| Bsp1407I | TGTACA | 1 cut(s) | 1193 |
| Bsp143I | GATC | 9 cut(s) | 158, 346, 443, 561, 1051, 1341, 1417, 1488, 1595 |
| Bsp68I | TCGCGA | 1 cut(s) | 948 |
| BspACI | CCGC | 1 cut(s) | 355 |
| BspANI | GGCC | 3 cut(s) | 122, 503, 753 |
| BspCNI | CTCAG | 3 cut(s) | 469, 1228, 1471 |
| BspDI | ATCGAT | 1 cut(s) | 1047 |
| BspFNI | CGCG | 1 cut(s) | 948 |
| BspHI | TCATGA | 3 cut(s) | 936, 1513, 1627 |
| BspLI | GGNNCC | 6 cut(s) | 39, 121, 280, 681, 821, 1419 |
| BspMAI | CTGCAG | 1 cut(s) | 362 |
| BspPI | GGATC | 4 cut(s) | 569, 1412, 1425, 1496 |
| BspT107I | GGYRCC | 2 cut(s) | 37, 679 |
| BsrFI | RCCGGY | 1 cut(s) | 753 |
| BsrGI | TGTACA | 1 cut(s) | 1193 |
| BsrI | ACTGG | 6 cut(s) | 148, 181, 236, 550, 647, 1838 |
| BssAI | RCCGGY | 1 cut(s) | 753 |
| BssECI | CCNNGG | 4 cut(s) | 553, 601, 1421, 1944 |
| BssMI | GATC | 9 cut(s) | 158, 346, 443, 561, 1051, 1341, 1417, 1488, 1595 |
| BssSI | CACGAG | 2 cut(s) | 392, 882 |
| BssT1I | CCWWGG | 1 cut(s) | 1944 |
| Bst2BI | CACGAG | 2 cut(s) | 392, 882 |
| Bst2UI | CCWGG | 4 cut(s) | 42, 276, 555, 602 |
| Bst4CI | ACNGT | 7 cut(s) | 115, 574, 656, 854, 1849, 1895, 1901 |
| Bst6I | CTCTTC | 3 cut(s) | 453, 1526, 1719 |
| BstAUI | TGTACA | 1 cut(s) | 1193 |
| BstC8I | GCNNGC | 6 cut(s) | 56, 139, 193, 334, 1120, 1305 |
| BstDEI | CTNAG | 7 cut(s) | 63, 375, 456, 1123, 1236, 1479, 1547 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 693 |
| BstF5I | GGATG | 2 cut(s) | 131, 192 |
| BstFNI | CGCG | 1 cut(s) | 948 |
| BstHHI | GCGC | 2 cut(s) | 69, 1750 |
| BstKTI | GATC | 9 cut(s) | 161, 349, 446, 564, 1054, 1344, 1420, 1491, 1598 |
| BstMAI | GTCTC | 1 cut(s) | 1475 |
| BstMBI | GATC | 9 cut(s) | 158, 346, 443, 561, 1051, 1341, 1417, 1488, 1595 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 64, 240, 1241, 1619 |
| BstNI | CCWGG | 4 cut(s) | 42, 276, 555, 602 |
| BstNSI | RCATGY | 3 cut(s) | 332, 746, 847 |
| BstSCI | CCNGG | 4 cut(s) | 40, 274, 553, 600 |
| BstSFI | CTRYAG | 1 cut(s) | 358 |
| BstUI | CGCG | 1 cut(s) | 948 |
| BstV1I | GCAGC | 7 cut(s) | 344, 372, 1232, 1319, 1373, 1625, 1928 |
| BstV2I | GAAGAC | 1 cut(s) | 974 |
| BstX2I | RGATCY | 3 cut(s) | 158, 1417, 1595 |
| BstYI | RGATCY | 3 cut(s) | 158, 1417, 1595 |
| Bsu15I | ATCGAT | 1 cut(s) | 1047 |
| BsuI | GTATCC | 1 cut(s) | 408 |
| BsuRI | GGCC | 3 cut(s) | 122, 503, 753 |
| BsuTUI | ATCGAT | 1 cut(s) | 1047 |
| BtsCI | GGATG | 2 cut(s) | 131, 192 |
| BtsIMutI | CAGTG | 4 cut(s) | 155, 543, 579, 1872 |
| BtuMI | TCGCGA | 1 cut(s) | 948 |
| Cac8I | GCNNGC | 6 cut(s) | 56, 139, 193, 334, 1120, 1305 |
| CaiI | CAGNNNCTG | 1 cut(s) | 601 |
| CciI | TCATGA | 3 cut(s) | 936, 1513, 1627 |
| CfoI | GCGC | 2 cut(s) | 69, 1750 |
| Cfr10I | RCCGGY | 1 cut(s) | 753 |
| Cfr13I | GGNCC | 4 cut(s) | 120, 352, 751, 1964 |
| ClaI | ATCGAT | 1 cut(s) | 1047 |
| CseI | GACGC | 1 cut(s) | 321 |
| CsiI | ACCWGGT | 1 cut(s) | 40 |
| DdeI | CTNAG | 7 cut(s) | 63, 375, 456, 1123, 1236, 1479, 1547 |
| DpnI | GATC | 9 cut(s) | 160, 348, 445, 563, 1053, 1343, 1419, 1490, 1597 |
| DpnII | GATC | 9 cut(s) | 158, 346, 443, 561, 1051, 1341, 1417, 1488, 1595 |
| DraIII | CACNNNGTG | 1 cut(s) | 412 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 564, 730 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 564, 730 |
| EaeI | YGGCCR | 1 cut(s) | 501 |
| Eam1104I | CTCTTC | 3 cut(s) | 453, 1526, 1719 |
| EarI | CTCTTC | 3 cut(s) | 453, 1526, 1719 |
| Eco130I | CCWWGG | 1 cut(s) | 1944 |
| Eco24I | GRGCYC | 1 cut(s) | 822 |
| Eco32I | GATATC | 1 cut(s) | 1678 |
| Eco47I | GGWCC | 2 cut(s) | 352, 1964 |
| Eco57I | CTGAAG | 3 cut(s) | 957, 1025, 1629 |
| Eco88I | CYCGRG | 3 cut(s) | 1526, 1743, 1925 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 693 |
| EcoRI | GAATTC | 1 cut(s) | 646 |
| EcoRII | CCWGG | 4 cut(s) | 40, 274, 553, 600 |
| EcoRV | GATATC | 1 cut(s) | 1678 |
| EcoT14I | CCWWGG | 1 cut(s) | 1944 |
| EcoT22I | ATGCAT | 1 cut(s) | 500 |
| EcoT38I | GRGCYC | 1 cut(s) | 822 |
| ErhI | CCWWGG | 1 cut(s) | 1944 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 325, 357, 807, 839 |
| FaqI | GGGAC | 1 cut(s) | 1079 |
| FauNDI | CATATG | 3 cut(s) | 1080, 1132, 1198 |
| FbaI | TGATCA | 2 cut(s) | 346, 1341 |
| FblI | GTMKAC | 1 cut(s) | 1552 |
| Fnu4HI | GCNGC | 7 cut(s) | 358, 361, 1221, 1308, 1387, 1614, 1917 |
| FokI | GGATG | 2 cut(s) | 138, 199 |
| FriOI | GRGCYC | 1 cut(s) | 822 |
| Fsp4HI | GCNGC | 7 cut(s) | 358, 361, 1221, 1308, 1387, 1614, 1917 |
| FspBI | CTAG | 3 cut(s) | 132, 321, 1026 |
| GlaI | GCGC | 2 cut(s) | 68, 1749 |
| GluI | GCNGC | 7 cut(s) | 358, 361, 1221, 1308, 1387, 1614, 1917 |
| GsaI | CCCAGC | 1 cut(s) | 917 |
| HaeIII | GGCC | 3 cut(s) | 122, 503, 753 |
| HapII | CCGG | 3 cut(s) | 123, 754, 1556 |
| HgaI | GACGC | 1 cut(s) | 321 |
| HhaI | GCGC | 2 cut(s) | 69, 1750 |
| Hin6I | GCGC | 2 cut(s) | 67, 1748 |
| HinP1I | GCGC | 2 cut(s) | 67, 1748 |
| HindIII | AAGCTT | 2 cut(s) | 334, 1037 |
| HinfI | GANTC | 9 cut(s) | 262, 535, 692, 1136, 1174, 1238, 1322, 1727, 1859 |
| HpaII | CCGG | 3 cut(s) | 123, 754, 1556 |
| HphI | GGTGA | 3 cut(s) | 710, 754, 1441 |
| Hpy166II | GTNNAC | 4 cut(s) | 577, 585, 762, 1553 |
| Hpy8I | GTNNAC | 4 cut(s) | 577, 585, 762, 1553 |
| Hpy99I | CGWCG | 1 cut(s) | 315 |
| HpyAV | CCTTC | 2 cut(s) | 1462, 1568 |
| HpyCH4III | ACNGT | 7 cut(s) | 115, 574, 656, 854, 1849, 1895, 1901 |
| HpyCH4IV | ACGT | 2 cut(s) | 473, 1227 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 64, 240, 1241, 1619 |
| HpyF3I | CTNAG | 7 cut(s) | 63, 375, 456, 1123, 1236, 1479, 1547 |
| HpySE526I | ACGT | 2 cut(s) | 473, 1227 |
| HspAI | GCGC | 2 cut(s) | 67, 1748 |
| KpnI | GGTACC | 2 cut(s) | 41, 683 |
| Ksp22I | TGATCA | 2 cut(s) | 346, 1341 |
| Kzo9I | GATC | 9 cut(s) | 158, 346, 443, 561, 1051, 1341, 1417, 1488, 1595 |
| LmnI | GCTCC | 4 cut(s) | 94, 284, 415, 825 |
| Lsp1109I | GCAGC | 7 cut(s) | 344, 372, 1232, 1319, 1373, 1625, 1928 |
| LweI | GCATC | 5 cut(s) | 280, 1207, 1290, 1527, 1631 |
| MabI | ACCWGGT | 1 cut(s) | 40 |
| MaeI | CTAG | 3 cut(s) | 132, 321, 1026 |
| MaeII | ACGT | 2 cut(s) | 473, 1227 |
| MaeIII | GTNAC | 5 cut(s) | 143, 469, 638, 698, 1895 |
| MalI | GATC | 9 cut(s) | 160, 348, 445, 563, 1053, 1343, 1419, 1490, 1597 |
| MboI | GATC | 9 cut(s) | 158, 346, 443, 561, 1051, 1341, 1417, 1488, 1595 |
| MboII | GAAGA | 9 cut(s) | 84, 440, 979, 998, 1178, 1258, 1513, 1643, 1736 |
| MfeI | CAATTG | 1 cut(s) | 1401 |
| MflI | RGATCY | 3 cut(s) | 158, 1417, 1595 |
| MhlI | GDGCHC | 3 cut(s) | 99, 328, 822 |
| MlsI | TGGCCA | 1 cut(s) | 503 |
| MluNI | TGGCCA | 1 cut(s) | 503 |
| MlyI | GAGTC | 5 cut(s) | 256, 1183, 1247, 1736, 1853 |
| Mox20I | TGGCCA | 1 cut(s) | 503 |
| Mph1103I | ATGCAT | 1 cut(s) | 500 |
| MscI | TGGCCA | 1 cut(s) | 503 |
| MseI | TTAA | 5 cut(s) | 206, 477, 617, 957, 1565 |
| Msp20I | TGGCCA | 1 cut(s) | 503 |
| MspA1I | CMGCKG | 2 cut(s) | 357, 1003 |
| MspI | CCGG | 3 cut(s) | 123, 754, 1556 |
| MspR9I | CCNGG | 4 cut(s) | 42, 276, 555, 602 |
| MunI | CAATTG | 1 cut(s) | 1401 |
| Mva1269I | GAATGC | 3 cut(s) | 500, 1480, 1548 |
| MvaI | CCWGG | 4 cut(s) | 42, 276, 555, 602 |
| MvnI | CGCG | 1 cut(s) | 948 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 64, 240, 1241, 1619 |
| NdeI | CATATG | 3 cut(s) | 1080, 1132, 1198 |
| NdeII | GATC | 9 cut(s) | 158, 346, 443, 561, 1051, 1341, 1417, 1488, 1595 |
| NlaIV | GGNNCC | 6 cut(s) | 39, 121, 280, 681, 821, 1419 |
| NmuCI | GTSAC | 2 cut(s) | 638, 698 |
| NruI | TCGCGA | 1 cut(s) | 948 |
| NsiI | ATGCAT | 1 cut(s) | 500 |
| NspI | RCATGY | 3 cut(s) | 332, 746, 847 |
| PaeR7I | CTCGAG | 2 cut(s) | 1526, 1925 |
| PagI | TCATGA | 3 cut(s) | 936, 1513, 1627 |
| PctI | GAATGC | 3 cut(s) | 500, 1480, 1548 |
| PfeI | GAWTC | 4 cut(s) | 535, 692, 1136, 1322 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1084, 1950 |
| PkrI | GCNGC | 7 cut(s) | 359, 362, 1222, 1309, 1388, 1615, 1918 |
| PleI | GAGTC | 5 cut(s) | 256, 1182, 1246, 1735, 1853 |
| PpsI | GAGTC | 5 cut(s) | 256, 1182, 1246, 1735, 1853 |
| PshBI | ATTAAT | 1 cut(s) | 1565 |
| PsiI | TTATAA | 1 cut(s) | 431 |
| Psp1406I | AACGTT | 2 cut(s) | 473, 1227 |
| Psp6I | CCWGG | 4 cut(s) | 40, 274, 553, 600 |
| PspFI | CCCAGC | 1 cut(s) | 913 |
| PspGI | CCWGG | 4 cut(s) | 40, 274, 553, 600 |
| PspN4I | GGNNCC | 6 cut(s) | 39, 121, 280, 681, 821, 1419 |
| PspPI | GGNCC | 4 cut(s) | 120, 352, 751, 1964 |
| PspXI | VCTCGAGB | 1 cut(s) | 1526 |
| PstI | CTGCAG | 1 cut(s) | 362 |
| PstNI | CAGNNNCTG | 1 cut(s) | 601 |
| PsuI | RGATCY | 3 cut(s) | 158, 1417, 1595 |
| PvuII | CAGCTG | 1 cut(s) | 1003 |
| RruI | TCGCGA | 1 cut(s) | 948 |
| SaqAI | TTAA | 5 cut(s) | 206, 477, 617, 957, 1565 |
| SatI | GCNGC | 7 cut(s) | 358, 361, 1221, 1308, 1387, 1614, 1917 |
| Sau3AI | GATC | 9 cut(s) | 158, 346, 443, 561, 1051, 1341, 1417, 1488, 1595 |
| Sau96I | GGNCC | 4 cut(s) | 120, 352, 751, 1964 |
| ScaI | AGTACT | 1 cut(s) | 1208 |
| SchI | GAGTC | 5 cut(s) | 256, 1183, 1247, 1736, 1853 |
| ScrFI | CCNGG | 4 cut(s) | 42, 276, 555, 602 |
| SduI | GDGCHC | 3 cut(s) | 99, 328, 822 |
| SexAI | ACCWGGT | 1 cut(s) | 40 |
| SfaNI | GCATC | 5 cut(s) | 280, 1207, 1290, 1527, 1631 |
| SfcI | CTRYAG | 1 cut(s) | 358 |
| Sfr274I | CTCGAG | 2 cut(s) | 1526, 1925 |
| SinI | GGWCC | 2 cut(s) | 352, 1964 |
| SlaI | CTCGAG | 2 cut(s) | 1526, 1925 |
| SmlI | CTYRAG | 2 cut(s) | 1526, 1925 |
| SmoI | CTYRAG | 2 cut(s) | 1526, 1925 |
| SsiI | CCGC | 1 cut(s) | 355 |
| SspMI | CTAG | 3 cut(s) | 132, 321, 1026 |
| StyD4I | CCNGG | 4 cut(s) | 40, 274, 553, 600 |
| StyI | CCWWGG | 1 cut(s) | 1944 |
| TaaI | ACNGT | 7 cut(s) | 115, 574, 656, 854, 1849, 1895, 1901 |
| TaiI | ACGT | 2 cut(s) | 476, 1230 |
| TaqI | TCGA | 5 cut(s) | 108, 1047, 1527, 1758, 1926 |
| TatI | WGTACW | 4 cut(s) | 128, 1193, 1206, 1787 |
| TfiI | GAWTC | 4 cut(s) | 535, 692, 1136, 1322 |
| Tru1I | TTAA | 5 cut(s) | 206, 477, 617, 957, 1565 |
| Tru9I | TTAA | 5 cut(s) | 206, 477, 617, 957, 1565 |
| TscAI | CASTG | 4 cut(s) | 155, 550, 579, 1879 |
| TseFI | GTSAC | 2 cut(s) | 638, 698 |
| TseI | GCWGC | 7 cut(s) | 357, 360, 1220, 1307, 1386, 1613, 1916 |
| Tsp45I | GTSAC | 2 cut(s) | 638, 698 |
| TspGWI | ACGGA | 1 cut(s) | 574 |
| TspRI | CASTG | 4 cut(s) | 155, 550, 579, 1879 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1084, 1950 |
| VpaK11BI | GGWCC | 2 cut(s) | 352, 1964 |
| VspI | ATTAAT | 1 cut(s) | 1565 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 693 |
| XapI | RAATTY | 6 cut(s) | 228, 433, 646, 992, 1284, 1461 |
| XceI | RCATGY | 3 cut(s) | 332, 746, 847 |
| XhoI | CTCGAG | 2 cut(s) | 1526, 1925 |
| XmiI | GTMKAC | 1 cut(s) | 1552 |
| XspI | CTAG | 3 cut(s) | 132, 321, 1026 |
| ZrmI | AGTACT | 1 cut(s) | 1208 |
| Zsp2I | ATGCAT | 1 cut(s) | 500 |