BED zinc finger
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1936 |
| Acc36I | ACCTGC | 2 cut(s) | 424, 1918 |
| AccB1I | GGYRCC | 1 cut(s) | 1490 |
| AccI | GTMKAC | 3 cut(s) | 106, 970, 1302 |
| AccII | CGCG | 2 cut(s) | 1026, 1813 |
| AccIII | TCCGGA | 1 cut(s) | 1868 |
| AciI | CCGC | 2 cut(s) | 1421, 1596 |
| AclWI | GGATC | 3 cut(s) | 139, 1427, 1578 |
| AcoI | YGGCCR | 1 cut(s) | 868 |
| AcsI | RAATTY | 4 cut(s) | 253, 1007, 1376, 1476 |
| AcuI | CTGAAG | 1 cut(s) | 513 |
| AfaI | GTAC | 5 cut(s) | 139, 644, 885, 1118, 1712 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1500, 1619 |
| AgsI | TTSAA | 6 cut(s) | 932, 1103, 1184, 1219, 1382, 1864 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 838 |
| AhlI | ACTAGT | 2 cut(s) | 140, 1673 |
| AjiI | CACGTC | 1 cut(s) | 1260 |
| AjnI | CCWGG | 4 cut(s) | 568, 1038, 1495, 1744 |
| Alw21I | GWGCWC | 4 cut(s) | 241, 1293, 1536, 1903 |
| Alw26I | GTCTC | 2 cut(s) | 825, 1266 |
| Alw44I | GTGCAC | 1 cut(s) | 1899 |
| AlwI | GGATC | 3 cut(s) | 139, 1427, 1578 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 17 |
| Aor13HI | TCCGGA | 1 cut(s) | 1868 |
| AoxI | GGCC | 3 cut(s) | 600, 868, 1498 |
| ApaLI | GTGCAC | 1 cut(s) | 1899 |
| ApeKI | GCWGC | 2 cut(s) | 893, 1309 |
| ApoI | RAATTY | 4 cut(s) | 253, 1007, 1376, 1476 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 139, 171 |
| AspA2I | CCTAGG | 1 cut(s) | 1784 |
| AspLEI | GCGC | 3 cut(s) | 1813, 1815, 1937 |
| AspS9I | GGNCC | 1 cut(s) | 600 |
| AsuHPI | GGTGA | 4 cut(s) | 95, 556, 953, 1055 |
| AvrII | CCTAGG | 1 cut(s) | 1784 |
| BaeGI | GKGCMC | 3 cut(s) | 1172, 1495, 1903 |
| BalI | TGGCCA | 1 cut(s) | 870 |
| BanI | GGYRCC | 1 cut(s) | 1490 |
| BanII | GRGCYC | 3 cut(s) | 241, 1293, 1536 |
| BbsI | GAAGAC | 1 cut(s) | 720 |
| Bbv12I | GWGCWC | 4 cut(s) | 241, 1293, 1536, 1903 |
| BbvI | GCAGC | 2 cut(s) | 905, 1321 |
| BccI | CCATC | 5 cut(s) | 605, 907, 1559, 1801, 1925 |
| BciT130I | CCWGG | 4 cut(s) | 570, 1040, 1497, 1746 |
| BciVI | GTATCC | 2 cut(s) | 1800, 1941 |
| BclI | TGATCA | 1 cut(s) | 259 |
| BcoDI | GTCTC | 2 cut(s) | 825, 1266 |
| BcuI | ACTAGT | 2 cut(s) | 140, 1673 |
| BfaI | CTAG | 9 cut(s) | 141, 938, 1061, 1209, 1614, 1632, 1674, 1785, 1961 |
| BfmI | CTRYAG | 2 cut(s) | 464, 1664 |
| BfuAI | ACCTGC | 2 cut(s) | 424, 1918 |
| BfuI | GTATCC | 2 cut(s) | 1800, 1941 |
| BglII | AGATCT | 1 cut(s) | 519 |
| BisI | GCNGC | 3 cut(s) | 894, 1310, 1422 |
| BlnI | CCTAGG | 1 cut(s) | 1784 |
| BlpI | GCTNAGC | 1 cut(s) | 1558 |
| BlsI | GCNGC | 3 cut(s) | 895, 1311, 1423 |
| BmcAI | AGTACT | 2 cut(s) | 139, 1712 |
| Bme1390I | CCNGG | 4 cut(s) | 570, 1040, 1497, 1746 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 838 |
| BmgBI | CACGTC | 1 cut(s) | 1260 |
| BmgT120I | GGNCC | 1 cut(s) | 600 |
| BmiI | GGNNCC | 2 cut(s) | 1492, 1722 |
| BmrFI | CCNGG | 4 cut(s) | 570, 1040, 1497, 1746 |
| BmsI | GCATC | 3 cut(s) | 4, 913, 1818 |
| BpiI | GAAGAC | 1 cut(s) | 720 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 509, 541 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1558 |
| BsaBI | GATNNNNATC | 3 cut(s) | 43, 762, 1659 |
| BsaJI | CCNNGG | 5 cut(s) | 1039, 1495, 1566, 1744, 1784 |
| BsaWI | WCCGGW | 1 cut(s) | 1868 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1500, 1619 |
| Bse1I | ACTGG | 3 cut(s) | 1458, 1651, 1949 |
| Bse3DI | GCAATG | 4 cut(s) | 871, 1070, 1082, 1822 |
| Bse8I | GATNNNNATC | 3 cut(s) | 43, 762, 1659 |
| BseAI | TCCGGA | 1 cut(s) | 1868 |
| BseBI | CCWGG | 4 cut(s) | 570, 1040, 1497, 1746 |
| BseDI | CCNNGG | 5 cut(s) | 1039, 1495, 1566, 1744, 1784 |
| BseGI | GGATG | 5 cut(s) | 283, 616, 928, 1833, 1917 |
| BseJI | GATNNNNATC | 3 cut(s) | 43, 762, 1659 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1500, 1619 |
| BseMI | GCAATG | 4 cut(s) | 871, 1070, 1082, 1822 |
| BseMII | CTCAG | 3 cut(s) | 139, 609, 1451 |
| BseNI | ACTGG | 3 cut(s) | 1458, 1651, 1949 |
| BsePI | GCGCGC | 1 cut(s) | 1811 |
| BseRI | GAGGAG | 1 cut(s) | 1384 |
| BseSI | GKGCMC | 3 cut(s) | 1172, 1495, 1903 |
| BseXI | GCAGC | 2 cut(s) | 905, 1321 |
| BsgI | GTGCAG | 2 cut(s) | 621, 912 |
| Bsh1236I | CGCG | 2 cut(s) | 1026, 1813 |
| BshFI | GGCC | 3 cut(s) | 602, 870, 1500 |
| BshNI | GGYRCC | 1 cut(s) | 1490 |
| BsiHKAI | GWGCWC | 4 cut(s) | 241, 1293, 1536, 1903 |
| BsiSI | CCGG | 2 cut(s) | 1501, 1869 |
| BslFI | GGGAC | 3 cut(s) | 160, 826, 1640 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1500, 1619 |
| BsmAI | GTCTC | 2 cut(s) | 825, 1266 |
| BsmBI | CGTCTC | 1 cut(s) | 1266 |
| BsmFI | GGGAC | 3 cut(s) | 160, 826, 1640 |
| BsmI | GAATGC | 1 cut(s) | 1859 |
| BsnI | GGCC | 3 cut(s) | 602, 870, 1500 |
| Bsp1286I | GDGCHC | 6 cut(s) | 241, 1172, 1293, 1495, 1536, 1903 |
| Bsp13I | TCCGGA | 1 cut(s) | 1868 |
| Bsp1407I | TGTACA | 1 cut(s) | 1116 |
| Bsp143I | GATC | 8 cut(s) | 131, 259, 382, 519, 763, 1432, 1570, 1885 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1558 |
| Bsp68I | TCGCGA | 1 cut(s) | 1026 |
| BspACI | CCGC | 2 cut(s) | 1421, 1596 |
| BspANI | GGCC | 3 cut(s) | 602, 870, 1500 |
| BspCNI | CTCAG | 3 cut(s) | 138, 610, 1450 |
| BspEI | TCCGGA | 1 cut(s) | 1868 |
| BspFNI | CGCG | 2 cut(s) | 1026, 1813 |
| BspHI | TCATGA | 1 cut(s) | 1285 |
| BspLI | GGNNCC | 2 cut(s) | 1492, 1722 |
| BspMAI | CTGCAG | 1 cut(s) | 1668 |
| BspMI | ACCTGC | 2 cut(s) | 424, 1918 |
| BspPI | GGATC | 3 cut(s) | 139, 1427, 1578 |
| BspQI | GCTCTTC | 1 cut(s) | 181 |
| BspT107I | GGYRCC | 1 cut(s) | 1490 |
| BsrDI | GCAATG | 4 cut(s) | 871, 1070, 1082, 1822 |
| BsrGI | TGTACA | 1 cut(s) | 1116 |
| BsrI | ACTGG | 3 cut(s) | 1458, 1651, 1949 |
| BssECI | CCNNGG | 5 cut(s) | 1039, 1495, 1566, 1744, 1784 |
| BssHII | GCGCGC | 1 cut(s) | 1811 |
| BssMI | GATC | 8 cut(s) | 131, 259, 382, 519, 763, 1432, 1570, 1885 |
| BssNAI | GTATAC | 2 cut(s) | 971, 1303 |
| BssT1I | CCWWGG | 2 cut(s) | 1566, 1784 |
| Bst1107I | GTATAC | 2 cut(s) | 971, 1303 |
| Bst2UI | CCWGG | 4 cut(s) | 570, 1040, 1497, 1746 |
| Bst4CI | ACNGT | 4 cut(s) | 512, 642, 975, 1201 |
| Bst6I | CTCTTC | 2 cut(s) | 181, 706 |
| BstAUI | TGTACA | 1 cut(s) | 1116 |
| BstC8I | GCNNGC | 3 cut(s) | 1024, 1610, 1813 |
| BstDEI | CTNAG | 5 cut(s) | 125, 618, 954, 1437, 1558 |
| BstF5I | GGATG | 5 cut(s) | 283, 616, 928, 1833, 1917 |
| BstFNI | CGCG | 2 cut(s) | 1026, 1813 |
| BstHHI | GCGC | 3 cut(s) | 1813, 1815, 1937 |
| BstKTI | GATC | 8 cut(s) | 134, 262, 385, 522, 766, 1435, 1573, 1888 |
| BstMAI | GTCTC | 2 cut(s) | 825, 1266 |
| BstMBI | GATC | 8 cut(s) | 131, 259, 382, 519, 763, 1432, 1570, 1885 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 932, 1130, 1421 |
| BstNI | CCWGG | 4 cut(s) | 570, 1040, 1497, 1746 |
| BstNSI | RCATGY | 1 cut(s) | 721 |
| BstSCI | CCNGG | 4 cut(s) | 568, 1038, 1495, 1744 |
| BstSFI | CTRYAG | 2 cut(s) | 464, 1664 |
| BstSLI | GKGCMC | 3 cut(s) | 1172, 1495, 1903 |
| BstUI | CGCG | 2 cut(s) | 1026, 1813 |
| BstV1I | GCAGC | 2 cut(s) | 905, 1321 |
| BstV2I | GAAGAC | 1 cut(s) | 720 |
| BstX2I | RGATCY | 2 cut(s) | 519, 1432 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1077 |
| BstYI | RGATCY | 2 cut(s) | 519, 1432 |
| BstZ17I | GTATAC | 2 cut(s) | 971, 1303 |
| BsuI | GTATCC | 2 cut(s) | 1800, 1941 |
| BsuRI | GGCC | 3 cut(s) | 602, 870, 1500 |
| BtrI | CACGTC | 1 cut(s) | 1260 |
| BtsCI | GGATG | 5 cut(s) | 283, 616, 928, 1833, 1917 |
| BtsI | GCAGTG | 1 cut(s) | 1960 |
| BtsIMutI | CAGTG | 4 cut(s) | 133, 1197, 1901, 1960 |
| BtuMI | TCGCGA | 1 cut(s) | 1026 |
| BveI | ACCTGC | 2 cut(s) | 424, 1918 |
| Cac8I | GCNNGC | 3 cut(s) | 1024, 1610, 1813 |
| CaiI | CAGNNNCTG | 1 cut(s) | 17 |
| CciI | TCATGA | 1 cut(s) | 1285 |
| CfoI | GCGC | 3 cut(s) | 1813, 1815, 1937 |
| Cfr13I | GGNCC | 1 cut(s) | 600 |
| Csp6I | GTAC | 5 cut(s) | 138, 643, 884, 1117, 1711 |
| CviAII | CATG | 6 cut(s) | 407, 691, 718, 781, 1286, 1834 |
| CviQI | GTAC | 5 cut(s) | 138, 643, 884, 1117, 1711 |
| DdeI | CTNAG | 5 cut(s) | 125, 618, 954, 1437, 1558 |
| DpnI | GATC | 8 cut(s) | 133, 261, 384, 521, 765, 1434, 1572, 1887 |
| DpnII | GATC | 8 cut(s) | 131, 259, 382, 519, 763, 1432, 1570, 1885 |
| DriI | GACNNNNNGTC | 1 cut(s) | 838 |
| EaeI | YGGCCR | 1 cut(s) | 868 |
| Eam1104I | CTCTTC | 2 cut(s) | 181, 706 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 838 |
| EarI | CTCTTC | 2 cut(s) | 181, 706 |
| Ecl136II | GAGCTC | 3 cut(s) | 239, 1291, 1534 |
| Eco130I | CCWWGG | 2 cut(s) | 1566, 1784 |
| Eco24I | GRGCYC | 3 cut(s) | 241, 1293, 1536 |
| Eco53kI | GAGCTC | 3 cut(s) | 239, 1291, 1534 |
| Eco57I | CTGAAG | 1 cut(s) | 513 |
| EcoICRI | GAGCTC | 3 cut(s) | 239, 1291, 1534 |
| EcoRII | CCWGG | 4 cut(s) | 568, 1038, 1495, 1744 |
| EcoT14I | CCWWGG | 2 cut(s) | 1566, 1784 |
| EcoT38I | GRGCYC | 3 cut(s) | 241, 1293, 1536 |
| ErhI | CCWWGG | 2 cut(s) | 1566, 1784 |
| Esp3I | CGTCTC | 1 cut(s) | 1266 |
| FaeI | CATG | 6 cut(s) | 410, 694, 721, 784, 1289, 1837 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 225, 257, 705, 737 |
| FaqI | GGGAC | 3 cut(s) | 160, 826, 1640 |
| FatI | CATG | 6 cut(s) | 406, 690, 717, 780, 1285, 1833 |
| FauNDI | CATATG | 2 cut(s) | 1055, 1090 |
| FbaI | TGATCA | 1 cut(s) | 259 |
| FblI | GTMKAC | 3 cut(s) | 106, 970, 1302 |
| Fnu4HI | GCNGC | 3 cut(s) | 894, 1310, 1422 |
| FokI | GGATG | 5 cut(s) | 290, 623, 935, 1840, 1904 |
| FriOI | GRGCYC | 3 cut(s) | 241, 1293, 1536 |
| Fsp4HI | GCNGC | 3 cut(s) | 894, 1310, 1422 |
| FspAI | RTGCGCAY | 1 cut(s) | 1936 |
| FspBI | CTAG | 9 cut(s) | 141, 938, 1061, 1209, 1614, 1632, 1674, 1785, 1961 |
| FspI | TGCGCA | 1 cut(s) | 1936 |
| GlaI | GCGC | 3 cut(s) | 1812, 1814, 1936 |
| GluI | GCNGC | 3 cut(s) | 894, 1310, 1422 |
| HaeIII | GGCC | 3 cut(s) | 602, 870, 1500 |
| HapII | CCGG | 2 cut(s) | 1501, 1869 |
| HhaI | GCGC | 3 cut(s) | 1813, 1815, 1937 |
| Hin1II | CATG | 6 cut(s) | 410, 694, 721, 784, 1289, 1837 |
| Hin6I | GCGC | 3 cut(s) | 1811, 1813, 1935 |
| HinP1I | GCGC | 3 cut(s) | 1811, 1813, 1935 |
| HincII | GTYRAC | 2 cut(s) | 33, 697 |
| HindII | GTYRAC | 2 cut(s) | 33, 697 |
| HindIII | AAGCTT | 1 cut(s) | 1842 |
| HpaII | CCGG | 2 cut(s) | 1501, 1869 |
| HphI | GGTGA | 4 cut(s) | 95, 556, 953, 1055 |
| Hpy166II | GTNNAC | 8 cut(s) | 33, 107, 697, 971, 1303, 1777, 1901, 1958 |
| Hpy8I | GTNNAC | 8 cut(s) | 33, 107, 697, 971, 1303, 1777, 1901, 1958 |
| HpyAV | CCTTC | 6 cut(s) | 227, 488, 535, 1182, 1617, 1679 |
| HpyCH4III | ACNGT | 4 cut(s) | 512, 642, 975, 1201 |
| HpyCH4IV | ACGT | 1 cut(s) | 1259 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 932, 1130, 1421 |
| HpyF3I | CTNAG | 5 cut(s) | 125, 618, 954, 1437, 1558 |
| HpySE526I | ACGT | 1 cut(s) | 1259 |
| Hsp92II | CATG | 6 cut(s) | 410, 694, 721, 784, 1289, 1837 |
| HspAI | GCGC | 3 cut(s) | 1811, 1813, 1935 |
| Kpn2I | TCCGGA | 1 cut(s) | 1868 |
| Ksp22I | TGATCA | 1 cut(s) | 259 |
| Kzo9I | GATC | 8 cut(s) | 131, 259, 382, 519, 763, 1432, 1570, 1885 |
| LguI | GCTCTTC | 1 cut(s) | 181 |
| LmnI | GCTCC | 2 cut(s) | 236, 1371 |
| Lsp1109I | GCAGC | 2 cut(s) | 905, 1321 |
| LweI | GCATC | 3 cut(s) | 4, 913, 1818 |
| MaeI | CTAG | 9 cut(s) | 141, 938, 1061, 1209, 1614, 1632, 1674, 1785, 1961 |
| MaeII | ACGT | 1 cut(s) | 1259 |
| MaeIII | GTNAC | 5 cut(s) | 83, 338, 353, 484, 1234 |
| MalI | GATC | 8 cut(s) | 133, 261, 384, 521, 765, 1434, 1572, 1887 |
| MboI | GATC | 8 cut(s) | 131, 259, 382, 519, 763, 1432, 1570, 1885 |
| MboII | GAAGA | 6 cut(s) | 198, 685, 693, 725, 1394, 1887 |
| MflI | RGATCY | 2 cut(s) | 519, 1432 |
| MhlI | GDGCHC | 6 cut(s) | 241, 1172, 1293, 1495, 1536, 1903 |
| MlsI | TGGCCA | 1 cut(s) | 870 |
| MluNI | TGGCCA | 1 cut(s) | 870 |
| MlyI | GAGTC | 4 cut(s) | 80, 692, 1622, 1847 |
| MmeI | TCCRAC | 3 cut(s) | 418, 1012, 1289 |
| Mox20I | TGGCCA | 1 cut(s) | 870 |
| MroI | TCCGGA | 1 cut(s) | 1868 |
| MscI | TGGCCA | 1 cut(s) | 870 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1075, 1930 |
| Msp20I | TGGCCA | 1 cut(s) | 870 |
| MspA1I | CMGCKG | 1 cut(s) | 1312 |
| MspI | CCGG | 2 cut(s) | 1501, 1869 |
| MspR9I | CCNGG | 4 cut(s) | 570, 1040, 1497, 1746 |
| Mva1269I | GAATGC | 1 cut(s) | 1859 |
| MvaI | CCWGG | 4 cut(s) | 570, 1040, 1497, 1746 |
| MvnI | CGCG | 2 cut(s) | 1026, 1813 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 932, 1130, 1421 |
| NdeI | CATATG | 2 cut(s) | 1055, 1090 |
| NdeII | GATC | 8 cut(s) | 131, 259, 382, 519, 763, 1432, 1570, 1885 |
| NlaIII | CATG | 6 cut(s) | 410, 694, 721, 784, 1289, 1837 |
| NlaIV | GGNNCC | 2 cut(s) | 1492, 1722 |
| NmeAIII | GCCGAG | 1 cut(s) | 1835 |
| NmuCI | GTSAC | 2 cut(s) | 83, 484 |
| NruI | TCGCGA | 1 cut(s) | 1026 |
| NsbI | TGCGCA | 1 cut(s) | 1936 |
| NspI | RCATGY | 1 cut(s) | 721 |
| PagI | TCATGA | 1 cut(s) | 1285 |
| PauI | GCGCGC | 1 cut(s) | 1811 |
| PciSI | GCTCTTC | 1 cut(s) | 181 |
| PctI | GAATGC | 1 cut(s) | 1859 |
| PfeI | GAWTC | 7 cut(s) | 163, 441, 653, 958, 1035, 1254, 1330 |
| PflFI | GACNNNGTC | 1 cut(s) | 1154 |
| PkrI | GCNGC | 3 cut(s) | 895, 1311, 1423 |
| PleI | GAGTC | 4 cut(s) | 80, 692, 1622, 1847 |
| PpsI | GAGTC | 4 cut(s) | 80, 692, 1622, 1847 |
| Psp124BI | GAGCTC | 3 cut(s) | 241, 1293, 1536 |
| Psp6I | CCWGG | 4 cut(s) | 568, 1038, 1495, 1744 |
| PspGI | CCWGG | 4 cut(s) | 568, 1038, 1495, 1744 |
| PspN4I | GGNNCC | 2 cut(s) | 1492, 1722 |
| PspPI | GGNCC | 1 cut(s) | 600 |
| PstI | CTGCAG | 1 cut(s) | 1668 |
| PstNI | CAGNNNCTG | 1 cut(s) | 17 |
| PsuI | RGATCY | 2 cut(s) | 519, 1432 |
| PsyI | GACNNNGTC | 1 cut(s) | 1154 |
| PteI | GCGCGC | 1 cut(s) | 1811 |
| PvuII | CAGCTG | 1 cut(s) | 1312 |
| RruI | TCGCGA | 1 cut(s) | 1026 |
| RsaI | GTAC | 5 cut(s) | 139, 644, 885, 1118, 1712 |
| RsaNI | GTAC | 5 cut(s) | 138, 643, 884, 1117, 1711 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1075, 1930 |
| SacI | GAGCTC | 3 cut(s) | 241, 1293, 1536 |
| SapI | GCTCTTC | 1 cut(s) | 181 |
| SatI | GCNGC | 3 cut(s) | 894, 1310, 1422 |
| Sau3AI | GATC | 8 cut(s) | 131, 259, 382, 519, 763, 1432, 1570, 1885 |
| Sau96I | GGNCC | 1 cut(s) | 600 |
| ScaI | AGTACT | 2 cut(s) | 139, 1712 |
| SchI | GAGTC | 4 cut(s) | 80, 692, 1622, 1847 |
| ScrFI | CCNGG | 4 cut(s) | 570, 1040, 1497, 1746 |
| SduI | GDGCHC | 6 cut(s) | 241, 1172, 1293, 1495, 1536, 1903 |
| SfaNI | GCATC | 3 cut(s) | 4, 913, 1818 |
| SfcI | CTRYAG | 2 cut(s) | 464, 1664 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1075, 1930 |
| SpeI | ACTAGT | 2 cut(s) | 140, 1673 |
| SsiI | CCGC | 2 cut(s) | 1421, 1596 |
| SspI | AATATT | 2 cut(s) | 301, 1388 |
| SspMI | CTAG | 9 cut(s) | 141, 938, 1061, 1209, 1614, 1632, 1674, 1785, 1961 |
| SstI | GAGCTC | 3 cut(s) | 241, 1293, 1536 |
| StyD4I | CCNGG | 4 cut(s) | 568, 1038, 1495, 1744 |
| StyI | CCWWGG | 2 cut(s) | 1566, 1784 |
| TaaI | ACNGT | 4 cut(s) | 512, 642, 975, 1201 |
| TaiI | ACGT | 1 cut(s) | 1262 |
| TaqI | TCGA | 3 cut(s) | 391, 961, 1011 |
| TatI | WGTACW | 4 cut(s) | 137, 642, 1116, 1710 |
| TauI | GCSGC | 1 cut(s) | 1424 |
| TfiI | GAWTC | 7 cut(s) | 163, 441, 653, 958, 1035, 1254, 1330 |
| TscAI | CASTG | 4 cut(s) | 133, 1204, 1908, 1960 |
| TseFI | GTSAC | 2 cut(s) | 83, 484 |
| TseI | GCWGC | 2 cut(s) | 893, 1309 |
| Tsp45I | GTSAC | 2 cut(s) | 83, 484 |
| TspDTI | ATGAA | 6 cut(s) | 294, 666, 676, 1274, 1463, 1836 |
| TspRI | CASTG | 4 cut(s) | 133, 1204, 1908, 1960 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1154 |
| VneI | GTGCAC | 1 cut(s) | 1899 |
| XapI | RAATTY | 4 cut(s) | 253, 1007, 1376, 1476 |
| XbaI | TCTAGA | 1 cut(s) | 1060 |
| XceI | RCATGY | 1 cut(s) | 721 |
| XmaJI | CCTAGG | 1 cut(s) | 1784 |
| XmiI | GTMKAC | 3 cut(s) | 106, 970, 1302 |
| XspI | CTAG | 9 cut(s) | 141, 938, 1061, 1209, 1614, 1632, 1674, 1785, 1961 |
| ZrmI | AGTACT | 2 cut(s) | 139, 1712 |