FvH4_6g01690

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000067
Physical Location & Seq
Forward (+)
959378 .. 966058
6681 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g01690.t1

Sequence Viewer

Length: 4431 bp
ATGGACGAGCCTTCCTCTTCTTCTTCGTTGCCCACAGATGTCTTCCTCAATTTTAGAGGCGAGGACACCCGAAACATCTTTGTCTGCCATCTTCACAGAGCTCTGGTACGGGAAACACTCCAAGTCTACATTGATAAGCATGATCTACAGAAAGGCGACGACCTTGAGAATCTACTGGAAGGCATTGCCGGATCAAAGCTTTCAATTTTAGTTTTCTCTGAAAACTACGCGACTTCCACTTGGTGCTTGAAAGAACTGGTGCAGATCCTGAAATGCAAGAAGGACCAGAACCAGATTGTGATGCCCGTTTTCTACAAAGTAGATCCTTCCGACATTCGTAAACAGAGGGGAAGTTTTGGTGAAGCTTTTGCTAAGCATGAAAGCGACTGCAAAGGAGACAAGGAGTTGCTGCAACAAGTGCAGAGCTGGAGATCCGCTTTAGAGGAAGCTGCCAACTTATCTGGCTTCGATTCCAATTCCAAAGATTATGCGGATGATGCCGGGCTTATTGATGCTATTGTCAAAAAAGTGTTCGATAAAGTGAACTCGATACCTCCTCATACAGAGGATGGCTTTGTTGCAATGGATTCCCATCTGAATGAAATGAGACGAAAAATAAATGCTGTGCCGGATGGTGTTGTCTATGTCGGAATATGTGGCATGGGCGGCATAGGCAAGACAACAATTGCTCATGCTGTTTTTAGAGAAATTGCTCATCAGTTTGACCACTGTTGCTTTCTTGAAGATGTGAGGGAACGCTTCACGAAGGGGGTAGTACAGTTGCAACGTGAATTTTTAGAAGGAATGTTCAAGAAAAAAGAACACAATGTGGACGCGGTCTACATGAAGATGATGAGAAGGCTAGCTAACAAAAAGATCCTAGTTGTTCTTGACGATGTACTCGATTCACGCCAGATGGATACTTTACTTGGTGGAAGTAATCGTTCATTTGGTGCTGGAAGTATAATTATTGTAACAACTAGAGATGAACATGTAGTGCGTGGATTCGAGATATACAAGCCCACCTCACTAAGTGTTCGCGATTCTCTGAAACTCTTCAGCCAGAATGCTTTCAGCACAGACAAACCCTCTGGAAAGTATGAACATCTCTCAAGGTGCATAGTACAATATGCACAGGGTCTACCCTTAGCTCTTATACTATTGGGAAAGTTACTCCGTAAGAGAAGTGTATCTGATTGGGAAGGTCAATTAAATAAATTGAGGAAGCAACCACACCCAGGAATTAAGGAGGTGCTTCAAATAAGCTTCGATGGACTACAAGATGAGTATCAGAAGAACATATTTCTGGATATTGCATGTTTCTTTAAAGGGATGGACAAAGACTATGTAACAATAGTTCTTGAAAATTGTGGCTTCTATCCCAGGGCGGACCTGAATGTTTTAGTTGAAAGAGCTCTTCTAACTATCTCAGATTCAAATAAGCTTGAGATGCATGATTTACTACACGAGATGGGGAGGTACATTGAATCCTCTACAGAAGATGGAAAGCGAAGACGATTCTGGAGTGAACATGTTGAGCGTGTGCTGACTGATAAAAATTCGGCGGCTACAGAAGCAGAAGGCTTGATGATCACCCTGCCGGACAAGGAAGACAGATTCTTCAAAGCCACAGCATTTGAACATATGACGAAACTGAGGTTGCTCATGTTCATGTACCGGGAATGGTACTATGGAGCTGACGGGAAACATGACCTGATTGGGGACTTCAACTTTCTTTCTTCTGAGTTGAGAGTTTTCATCTGGCATAAATACCCCCTACAGTGTTTGCCATCCAGCTTTGACCCCAAGAATCTTGTTCACCTTGACATGCACTCCGGCCGCATTGAACACCTTTGGGAAGGACCTAAGCGTGCGAACAACTTGAGAATCATCAATTTGATTGGCTGTCCACTTAAAGAGACTCCCGACTTGACTGAGGTGCCAAATCTTGAGAGCCTAGTTATGGGAGGGGGTATTATTGGAGGTCGCCCAAGTTTCACTGAGGTTCACCCATCCATTTTAGGTCACGAAAACCTTGTATTTTTGAGCTTAGCTGGGTGCCATGAACTTAAGACACTTCCGAGAAGAATTCTCATGAAGTCTCTCAAAACCCTTGATCTTAGCCACTGTGTAAACCTTGAGATGTTTCCAGAGATTTCTGAAGATATGAAAGCACTGTCAGTGCTTAGCTTATATCAAACTGCAATTATGGAGCTACCATCGTCAATTGAACGGCTCCGGGGGCTTCAATCATTAGACATGACATTCTGCAGGAAACTTATCCGTCTTCCAGATAGTATCTGTAATTTAGCAAAACTTAGATCTCTCAAGCTGAAAGATTGCTCAAAACTTTCCAAGTTGCCTGAGAACATTGGGAATATGGATTCTCTGTTGGAACTTGAAGTAGACCTAGGTGGTTTAGAACAACTTCCAGTCTCTATCTTACGTCTGAAGTTAGGTGGGCGGCTATCGTTTCATGGTTGCAAAATGGCGGCTCCCTTTTCATCATGGCCATTATCAATAGAAGATCGTTGTACTGATGTGGTACATCTTGATTTCAGCGGTTGCAATATGATGAAATTGTCAGATGCTATTGCTTGTTTTCCTTCATTGAAAGTATTAAAGTTGTCAAGAAACAATTTGGAGCGCTTACCTGCAGCCATGAAAGAACTGGGTTGCTTAGAAAGGCTTGAATTGGATGGCTGCAAGAGACTGAGATCAATACCAGAGCTTTCGTCAACAATAAGTTACATAAATGCACAAAACTGCACAGCTTTGGAAACGGTTTCGGCACCACAGTCTCCCTATGAAATTGGTCGGTGCTTCATATTTTCTAATTGCTGTAATCTGGTACATGAAGATGTTTTTAAAGACATTGTAGGAACTCATTTCCCCCCTCAGGGCAATCCTTCCCGACCCTTTTATTTCTCTTGTCCTGGAAGTGAAATTCTAGGGCAGTTCATCCATAGTGAGGGGTCTTCCGTAACTGCCAAGCTACCACCAAATTGCTTTGACAACAAGTTTTTGGGGTTCGCTATAACCGCCATTACTAATAACCCCCAAGGTGTTGATTACTACAATTTGATTGTTCGATGTTTCTTCACCTTCAAAGGAGATCAATGCCAGTACCGTTCCAGTTTCTATTTGTTCGATAGTCATTTTGACAGTCTATGCCGCGATTGGCTCAAGTCAAGTCATATGTTGGTAGGATATGTGCCATGGTCTGAATTTGGCATTAATGGAGAAAAAGTGAACGAACGTCACTACACTGAGGCCAAATTTGAGATAGAATTATTGCACTCTGAAGTGGGTGATGTGGTGATGACCAAGGCAAGATTCGACCCCAGCATCGAAAGGTGTTTCCCGGGCATCAAAAGGTGCGGAGTTCAATTCTTTTTTGCCAACAATGAGGATAAGGAAGTTATATATCAAGATTTTGGGGAACCAATGGTACAAGTCCTTGATAATTCTGAGATCAGGAGCCTTGAGGGCTGCTCAGCTGAAGCCAGTTGTAGTGAAATCACTGGTCACCCTTCCGATGAGGAGGAGCGATTCCTTAAATTATCCGAAGTTTTTGAAGGTGCATACAGAAAACCCAGCCGTTACATGAAAACTGAGGACAACGAGGATTCATCTGGGAAGAGGGAAACTTTGAAAAATTGGAAGCAAAAGGTGAAAGTTACATCAGGTCTGACGCAAGGGCATCTCGATGATGGTTTTAACTGGTCCATTGGGAGCTCGGGGATTGAGAGATGTAAGTATCGATGTGCTCATGGACTTGGCTGTTGGGCGAAAGCCATAGTGCCAACTCCCATCGACCCAACCATCCGTAACATTACTTATGTCGGGAGGCACACTTGTTGGAAAACTCAACCAATGTTGACACACCAAGTAAGAATTACACTGGGTATGGAGACTATTGGAGGTCCTTTTGATGGCTTTCGGTGGACGAAGTATGACCAAAGGGACATACTAGGAAGCAGATACCCAAGCAGAGAGTATTACGTATGCAATCCTGAAGAATACGGAGGCTGCATGGCCGTAAAGGAAGTGCAAAAATCAAATGATCAGAGATTCCTTAGAATTACTTACACCGGAAGGCACACATGTACGCTACCCTCCACGGGCATCGGAGGGAGGACTGATAGCAGTACCTCCTTTGCCAGTAGTGCATCATCAGGTTCTAGTGAAGAAATTGCCGAATTACTGAGATCACTAGGACCGGATCTTGAAGGGATAGTCAACTTTCTTAACAATGCTGACAATCAGATTGCATTACGAGATGAATTCTCACGGGCTAGGGGTGTCAATGATATGGAGAATAATAATAACAACGTGACAGGCTGTTTATGCTGGTTCAGGAGTCTCTGGAATTTCATGACCTCAAAGAAAAATACCCCAGAAATCCATGAAGACGAGGAAGGCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1477

Amino Acids

167.21

Weight (kDa)

6.1

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 13 - 189 2.1e-47 TIR domain
TIR_2 PF13676 14 - 109 1.2e-13 TIR domain
NB-ARC PF00931 211 - 360 2.5e-20 NB-ARC domain
WHD_ROQ1 PF23282 429 - 495 1.4e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 698 - 802 4e-11 Leucine-rich repeat region
LRR_13 PF23286 699 - 785 9.4e-13 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 765 - 818 7.2e-06 Leucine-rich repeat region
LRR_13 PF23286 786 - 932 1.7e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 979 - 1135 9e-07 C-JID domain
WRKY PF03106 1312 - 1370 6e-15 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2662
AccB1I GGYRCC 3 cut(s) 1939, 2058, 2791
AccI GTMKAC 4 cut(s) 126, 840, 1141, 2406
AccII CGCG 4 cut(s) 230, 836, 1041, 3177
AclWI GGATC 6 cut(s) 199, 259, 317, 426, 871, 4233
AcoI YGGCCR 3 cut(s) 1837, 2510, 4038
AcsI RAATTY 8 cut(s) 791, 1558, 2088, 2946, 3227, 3278, 4286, 4372
AcuI CTGAAG 6 cut(s) 1042, 2181, 2471, 3324, 3522, 4038
AdeI CACNNNGTG 3 cut(s) 243, 829, 1034
AfeI AGCGCT 1 cut(s) 2648
AfiI CCNNNNNNNGG 8 cut(s) 1238, 1720, 1963, 2899, 2900, 2971, 3935, 4124
AflII CTTAAG 1 cut(s) 2069
AflIII ACRYGT 3 cut(s) 991, 1531, 4106
AjnI CCWGG 3 cut(s) 1237, 1382, 2935
AjuI GAANNNNNNNTTGG 2 cut(s) 3320, 3352
Alw21I GWGCWC 4 cut(s) 103, 1417, 3740, 3772
Alw26I GTCTC 9 cut(s) 390, 601, 1913, 2106, 2440, 2704, 2805, 3908, 4370
AlwI GGATC 6 cut(s) 199, 259, 317, 426, 871, 4233
AlwNI CAGNNNCTG 1 cut(s) 268
Ama87I CYCGRG 2 cut(s) 3364, 3739
Aor51HI AGCGCT 1 cut(s) 2648
AoxI GGCC 5 cut(s) 1837, 2510, 3273, 4038, 4423
ApeKI GCWGC 6 cut(s) 409, 449, 2657, 2703, 3492, 4032
ApoI RAATTY 8 cut(s) 791, 1558, 2088, 2946, 3227, 3278, 4286, 4372
AseI ATTAAT 1 cut(s) 3237
Asp700I GAANNNNTTC 3 cut(s) 1055, 1070, 2427
AspA2I CCTAGG 1 cut(s) 2410
AspLEI GCGC 1 cut(s) 2649
AspS9I GGNCC 6 cut(s) 283, 1390, 1862, 3726, 3926, 4220
AsuC2I CCSGG 5 cut(s) 502, 1679, 2240, 3365, 3366
AsuHPI GGTGA 9 cut(s) 371, 1585, 1811, 2000, 3094, 3323, 3331, 3521, 3685
AsuNHI GCTAGC 1 cut(s) 862
AvaI CYCGRG 2 cut(s) 3364, 3739
AvaII GGWCC 6 cut(s) 283, 1390, 1862, 3726, 3926, 4220
AvrII CCTAGG 1 cut(s) 2410
AxyI CCTNAGG 1 cut(s) 2898
BalI TGGCCA 1 cut(s) 2512
BanI GGYRCC 3 cut(s) 1939, 2058, 2791
BanII GRGCYC 3 cut(s) 103, 1417, 3740
BauI CACGAG 1 cut(s) 1466
BbsI GAAGAC 6 cut(s) 34, 1519, 1617, 2279, 2970, 4419
Bbv12I GWGCWC 4 cut(s) 103, 1417, 3740, 3772
BbvI GCAGC 6 cut(s) 396, 436, 2669, 2690, 3479, 4019
BceAI ACGGC 3 cut(s) 2249, 3585, 4025
BciT130I CCWGG 3 cut(s) 1239, 1384, 2937
BciVI GTATCC 1 cut(s) 913
BclI TGATCA 2 cut(s) 1590, 4066
BcnI CCSGG 5 cut(s) 502, 1679, 2240, 3365, 3366
BcoDI GTCTC 9 cut(s) 390, 601, 1913, 2106, 2440, 2704, 2805, 3908, 4370
BfmI CTRYAG 6 cut(s) 146, 1494, 1569, 1778, 2269, 2655
BfoI RGCGCY 1 cut(s) 2650
BfrI CTTAAG 1 cut(s) 2069
BfuAI ACCTGC 1 cut(s) 2662
BfuI GTATCC 1 cut(s) 913
BglI GCCNNNNNGGC 1 cut(s) 3489
BglII AGATCT 1 cut(s) 2321
BlnI CCTAGG 1 cut(s) 2410
BlpI GCTNAGC 4 cut(s) 372, 2050, 2186, 3496
Bme1390I CCNGG 8 cut(s) 502, 1239, 1384, 1679, 2240, 2937, 3365, 3366
Bme18I GGWCC 6 cut(s) 283, 1390, 1862, 3726, 3926, 4220
BmeT110I CYCGRG 2 cut(s) 3364, 3739
BmgT120I GGNCC 6 cut(s) 283, 1390, 1862, 3726, 3926, 4220
BmiI GGNNCC 7 cut(s) 1941, 2060, 2237, 2496, 2793, 3444, 3482
BmrFI CCNGG 8 cut(s) 502, 1239, 1384, 1679, 2240, 2937, 3365, 3366
BmrI ACTGGG 2 cut(s) 2681, 3914
BmtI GCTAGC 1 cut(s) 866
BmuI ACTGGG 2 cut(s) 2681, 3914
BpiI GAAGAC 6 cut(s) 34, 1519, 1617, 2279, 2970, 4419
BplI GAGNNNNNCTC 3 cut(s) 31, 3479, 3511
BpmI CTGGAG 2 cut(s) 448, 1543
Bpu10I CCTNAGC 2 cut(s) 1147, 1866
Bpu1102I GCTNAGC 4 cut(s) 372, 2050, 2186, 3496
BpuEI CTTGAG 9 cut(s) 185, 1096, 1466, 1903, 1970, 2159, 2312, 3170, 3506
BpuMI CCSGG 5 cut(s) 502, 1679, 2240, 3365, 3366
Bsa29I ATCGAT 1 cut(s) 3763
BsaAI YACGTR 1 cut(s) 4006
BsaBI GATNNNNATC 2 cut(s) 591, 1287
BsaWI WCCGGW 2 cut(s) 4094, 4222
BsaXI ACNNNNNCTCC 4 cut(s) 1817, 1847, 3234, 3264
Bsc4I CCNNNNNNNGG 8 cut(s) 1238, 1720, 1963, 2899, 2900, 2971, 3935, 4124
Bse21I CCTNAGG 1 cut(s) 2898
Bse3DI GCAATG 2 cut(s) 183, 588
Bse8I GATNNNNATC 2 cut(s) 591, 1287
BseBI CCWGG 3 cut(s) 1239, 1384, 2937
BseCI ATCGAT 1 cut(s) 3763
BseGI GGATG 9 cut(s) 499, 574, 637, 1338, 1790, 2012, 2704, 2961, 3825
BseJI GATNNNNATC 2 cut(s) 591, 1287
BseLI CCNNNNNNNGG 8 cut(s) 1238, 1720, 1963, 2899, 2900, 2971, 3935, 4124
BseMI GCAATG 2 cut(s) 183, 588
BseRI GAGGAG 3 cut(s) 546, 3557, 3560
BseX3I CGGCCG 1 cut(s) 1837
BseXI GCAGC 6 cut(s) 396, 436, 2669, 2690, 3479, 4019
BseYI CCCAGC 3 cut(s) 2054, 3344, 3596
BsgI GTGCAG 3 cut(s) 281, 440, 2752
Bsh1236I CGCG 4 cut(s) 230, 836, 1041, 3177
Bsh1285I CGRYCG 1 cut(s) 1840
BshFI GGCC 5 cut(s) 1839, 2512, 3275, 4040, 4425
BshNI GGYRCC 3 cut(s) 1939, 2058, 2791
BshVI ATCGAT 1 cut(s) 3763
BsiEI CGRYCG 1 cut(s) 1840
BsiHKAI GWGCWC 4 cut(s) 103, 1417, 3740, 3772
BsiHKCI CYCGRG 2 cut(s) 3364, 3739
BslFI GGGAC 2 cut(s) 1736, 3980
BslI CCNNNNNNNGG 8 cut(s) 1238, 1720, 1963, 2899, 2900, 2971, 3935, 4124
BsmAI GTCTC 9 cut(s) 390, 601, 1913, 2106, 2440, 2704, 2805, 3908, 4370
BsmBI CGTCTC 1 cut(s) 601
BsmFI GGGAC 2 cut(s) 1736, 3980
BsmI GAATGC 1 cut(s) 1072
BsnI GGCC 5 cut(s) 1839, 2512, 3275, 4040, 4425
BsoBI CYCGRG 2 cut(s) 3364, 3739
Bsp1286I GDGCHC 4 cut(s) 103, 1417, 3740, 3772
Bsp1720I GCTNAGC 4 cut(s) 372, 2050, 2186, 3496
Bsp19I CCATGG 1 cut(s) 3218
Bsp68I TCGCGA 1 cut(s) 1041
BspANI GGCC 5 cut(s) 1839, 2512, 3275, 4040, 4425
BspDI ATCGAT 1 cut(s) 3763
BspFNI CGCG 4 cut(s) 230, 836, 1041, 3177
BspHI TCATGA 2 cut(s) 2094, 4377
BspLI GGNNCC 7 cut(s) 1941, 2060, 2237, 2496, 2793, 3444, 3482
BspMAI CTGCAG 2 cut(s) 2273, 2659
BspMI ACCTGC 1 cut(s) 2662
BspOI GCTAGC 1 cut(s) 866
BspPI GGATC 6 cut(s) 199, 259, 317, 426, 871, 4233
BspQI GCTCTTC 1 cut(s) 1422
BspT107I GGYRCC 3 cut(s) 1939, 2058, 2791
BspTI CTTAAG 1 cut(s) 2069
BsrDI GCAATG 2 cut(s) 183, 588
BssSI CACGAG 1 cut(s) 1466
BssT1I CCWWGG 4 cut(s) 2410, 3061, 3218, 3327
Bst2BI CACGAG 1 cut(s) 1466
Bst2UI CCWGG 3 cut(s) 1239, 1384, 2937
Bst4CI ACNGT 9 cut(s) 731, 780, 1782, 2129, 2178, 2785, 2799, 3131, 3167
Bst6I CTCTTC 4 cut(s) 22, 1061, 1422, 3635
BstAFI CTTAAG 1 cut(s) 2069
BstAPI GCANNNNNTGC 1 cut(s) 418
BstBAI YACGTR 1 cut(s) 4006
BstC8I GCNNGC 2 cut(s) 864, 1872
BstDSI CCRYGG 2 cut(s) 3218, 4122
BstEII GGTNACC 1 cut(s) 3527
BstF5I GGATG 9 cut(s) 499, 574, 637, 1338, 1790, 2012, 2704, 2961, 3825
BstFNI CGCG 4 cut(s) 230, 836, 1041, 3177
BstH2I RGCGCY 1 cut(s) 2650
BstHHI GCGC 1 cut(s) 2649
BstMAI GTCTC 9 cut(s) 390, 601, 1913, 2106, 2440, 2704, 2805, 3908, 4370
BstMCI CGRYCG 1 cut(s) 1840
BstNI CCWGG 3 cut(s) 1239, 1384, 2937
BstNSI RCATGY 5 cut(s) 995, 1320, 1535, 1831, 4110
BstPI GGTNACC 1 cut(s) 3527
BstSCI CCNGG 8 cut(s) 500, 1237, 1382, 1677, 2238, 2935, 3363, 3364
BstSFI CTRYAG 6 cut(s) 146, 1494, 1569, 1778, 2269, 2655
BstSNI TACGTA 1 cut(s) 4006
BstUI CGCG 4 cut(s) 230, 836, 1041, 3177
BstV1I GCAGC 6 cut(s) 396, 436, 2669, 2690, 3479, 4019
BstV2I GAAGAC 6 cut(s) 34, 1519, 1617, 2279, 2970, 4419
BstX2I RGATCY 6 cut(s) 264, 322, 431, 876, 2321, 4225
BstYI RGATCY 6 cut(s) 264, 322, 431, 876, 2321, 4225
BstZI CGGCCG 1 cut(s) 1837
Bsu15I ATCGAT 1 cut(s) 3763
Bsu36I CCTNAGG 1 cut(s) 2898
BsuI GTATCC 1 cut(s) 913
BsuRI GGCC 5 cut(s) 1839, 2512, 3275, 4040, 4425
BsuTUI ATCGAT 1 cut(s) 3763
BtgI CCRYGG 2 cut(s) 3218, 4122
BtsCI GGATG 9 cut(s) 499, 574, 637, 1338, 1790, 2012, 2704, 2961, 3825
BtsIMutI CAGTG 9 cut(s) 727, 1787, 1998, 2125, 2174, 2187, 3267, 3522, 3902
BtuMI TCGCGA 1 cut(s) 1041
BveI ACCTGC 1 cut(s) 2662
Cac8I GCNNGC 2 cut(s) 864, 1872
CaiI CAGNNNCTG 1 cut(s) 268
CciI TCATGA 2 cut(s) 2094, 4377
CfoI GCGC 1 cut(s) 2649
Cfr13I GGNCC 6 cut(s) 283, 1390, 1862, 3726, 3926, 4220
Cfr9I CCCGGG 1 cut(s) 3364
ClaI ATCGAT 1 cut(s) 3763
CseI GACGC 2 cut(s) 842, 3703
CspCI CAANNNNNGTGG 2 cut(s) 1618, 1653
DraI TTTAAA 2 cut(s) 1327, 2869
DraIII CACNNNGTG 3 cut(s) 243, 829, 1034
EaeI YGGCCR 3 cut(s) 1837, 2510, 4038
EagI CGGCCG 1 cut(s) 1837
Eam1104I CTCTTC 4 cut(s) 22, 1061, 1422, 3635
EarI CTCTTC 4 cut(s) 22, 1061, 1422, 3635
EciI GGCGGA 1 cut(s) 1403
Ecl136II GAGCTC 3 cut(s) 101, 1415, 3738
EclXI CGGCCG 1 cut(s) 1837
Eco105I TACGTA 1 cut(s) 4006
Eco130I CCWWGG 4 cut(s) 2410, 3061, 3218, 3327
Eco24I GRGCYC 3 cut(s) 103, 1417, 3740
Eco47I GGWCC 6 cut(s) 283, 1390, 1862, 3726, 3926, 4220
Eco47III AGCGCT 1 cut(s) 2648
Eco52I CGGCCG 1 cut(s) 1837
Eco53kI GAGCTC 3 cut(s) 101, 1415, 3738
Eco57I CTGAAG 6 cut(s) 1042, 2181, 2471, 3324, 3522, 4038
Eco81I CCTNAGG 1 cut(s) 2898
Eco88I CYCGRG 2 cut(s) 3364, 3739
Eco91I GGTNACC 1 cut(s) 3527
EcoICRI GAGCTC 3 cut(s) 101, 1415, 3738
EcoO109I RGGNCCY 2 cut(s) 1862, 3926
EcoO65I GGTNACC 1 cut(s) 3527
EcoRI GAATTC 2 cut(s) 2088, 4286
EcoRII CCWGG 3 cut(s) 1237, 1382, 2935
EcoT14I CCWWGG 4 cut(s) 2410, 3061, 3218, 3327
EcoT22I ATGCAT 1 cut(s) 1455
EcoT38I GRGCYC 3 cut(s) 103, 1417, 3740
ErhI CCWWGG 4 cut(s) 2410, 3061, 3218, 3327
Esp3I CGTCTC 1 cut(s) 601
FaqI GGGAC 2 cut(s) 1736, 3980
FauNDI CATATG 2 cut(s) 1644, 3198
FbaI TGATCA 2 cut(s) 1590, 4066
FblI GTMKAC 4 cut(s) 126, 840, 1141, 2406
FokI GGATG 9 cut(s) 506, 581, 644, 1345, 1777, 1999, 2711, 2948, 3812
FriOI GRGCYC 3 cut(s) 103, 1417, 3740
GlaI GCGC 1 cut(s) 2648
GsaI CCCAGC 3 cut(s) 2058, 3348, 3600
GsuI CTGGAG 2 cut(s) 448, 1543
HaeII RGCGCY 1 cut(s) 2650
HaeIII GGCC 5 cut(s) 1839, 2512, 3275, 4040, 4425
HgaI GACGC 2 cut(s) 842, 3703
HhaI GCGC 1 cut(s) 2649
Hin6I GCGC 1 cut(s) 2647
HinP1I GCGC 1 cut(s) 2647
HincII GTYRAC 3 cut(s) 2739, 3882, 4243
HindII GTYRAC 3 cut(s) 2739, 3882, 4243
HindIII AAGCTT 4 cut(s) 197, 363, 1264, 1442
HphI GGTGA 9 cut(s) 371, 1585, 1811, 2000, 3094, 3323, 3331, 3521, 3685
Hpy99I CGWCG 1 cut(s) 161
HpyCH4III ACNGT 9 cut(s) 731, 780, 1782, 2129, 2178, 2785, 2799, 3131, 3167
HpyCH4IV ACGT 5 cut(s) 787, 2446, 3259, 4005, 4335
HpySE526I ACGT 5 cut(s) 787, 2446, 3259, 4005, 4335
HspAI GCGC 1 cut(s) 2647
Ksp22I TGATCA 2 cut(s) 1590, 4066
LguI GCTCTTC 1 cut(s) 1422
LmnI GCTCC 8 cut(s) 1694, 2212, 2241, 2500, 2644, 3480, 3547, 3735
Lsp1109I GCAGC 6 cut(s) 396, 436, 2669, 2690, 3479, 4019
MaeII ACGT 5 cut(s) 787, 2446, 3259, 4005, 4335
MfeI CAATTG 2 cut(s) 684, 2226
MflI RGATCY 6 cut(s) 264, 322, 431, 876, 2321, 4225
MhlI GDGCHC 4 cut(s) 103, 1417, 3740, 3772
MlsI TGGCCA 1 cut(s) 2512
MluNI TGGCCA 1 cut(s) 2512
MlyI GAGTC 2 cut(s) 1915, 4372
MmeI TCCRAC 4 cut(s) 354, 628, 2373, 3842
Mox20I TGGCCA 1 cut(s) 2512
Mph1103I ATGCAT 1 cut(s) 1455
MroXI GAANNNNTTC 3 cut(s) 1055, 1070, 2427
MscI TGGCCA 1 cut(s) 2512
MslI CAYNNNNRTG 5 cut(s) 597, 848, 1670, 2859, 3708
Msp20I TGGCCA 1 cut(s) 2512
MspA1I CMGCKG 2 cut(s) 2562, 3500
MspCI CTTAAG 1 cut(s) 2069
MspR9I CCNGG 8 cut(s) 502, 1239, 1384, 1679, 2240, 2937, 3365, 3366
MunI CAATTG 2 cut(s) 684, 2226
Mva1269I GAATGC 1 cut(s) 1072
MvaI CCWGG 3 cut(s) 1239, 1384, 2937
MvnI CGCG 4 cut(s) 230, 836, 1041, 3177
NciI CCSGG 5 cut(s) 502, 1679, 2240, 3365, 3366
NcoI CCATGG 1 cut(s) 3218
NdeI CATATG 2 cut(s) 1644, 3198
NheI GCTAGC 1 cut(s) 862
NlaIV GGNNCC 7 cut(s) 1941, 2060, 2237, 2496, 2793, 3444, 3482
NmuCI GTSAC 4 cut(s) 2024, 3260, 3527, 4336
NruI TCGCGA 1 cut(s) 1041
NsiI ATGCAT 1 cut(s) 1455
NspI RCATGY 5 cut(s) 995, 1320, 1535, 1831, 4110
PagI TCATGA 2 cut(s) 2094, 4377
PasI CCCWGGG 1 cut(s) 1383
PciI ACATGT 3 cut(s) 991, 1531, 4106
PciSI GCTCTTC 1 cut(s) 1422
PcsI WCGNNNNNNNCGW 1 cut(s) 900
PctI GAATGC 1 cut(s) 1072
PdmI GAANNNNTTC 3 cut(s) 1055, 1070, 2427
PflFI GACNNNGTC 1 cut(s) 836
PfoI TCCNGGA 1 cut(s) 2935
PleI GAGTC 2 cut(s) 1915, 4371
PpsI GAGTC 2 cut(s) 1915, 4371
Ppu21I YACGTR 1 cut(s) 4006
PpuMI RGGWCCY 2 cut(s) 1862, 3926
PscI ACATGT 3 cut(s) 991, 1531, 4106
PshBI ATTAAT 1 cut(s) 3237
Psp124BI GAGCTC 3 cut(s) 103, 1417, 3740
Psp5II RGGWCCY 2 cut(s) 1862, 3926
Psp6I CCWGG 3 cut(s) 1237, 1382, 2935
PspEI GGTNACC 1 cut(s) 3527
PspFI CCCAGC 3 cut(s) 2054, 3344, 3596
PspGI CCWGG 3 cut(s) 1237, 1382, 2935
PspN4I GGNNCC 7 cut(s) 1941, 2060, 2237, 2496, 2793, 3444, 3482
PspPI GGNCC 6 cut(s) 283, 1390, 1862, 3726, 3926, 4220
PspPPI RGGWCCY 2 cut(s) 1862, 3926
PsrI GAACNNNNNNTAC 4 cut(s) 1341, 1373, 3435, 3467
PstI CTGCAG 2 cut(s) 2273, 2659
PstNI CAGNNNCTG 1 cut(s) 268
PsuI RGATCY 6 cut(s) 264, 322, 431, 876, 2321, 4225
PsyI GACNNNGTC 1 cut(s) 836
PvuII CAGCTG 1 cut(s) 3500
RruI TCGCGA 1 cut(s) 1041
RseI CAYNNNNRTG 5 cut(s) 597, 848, 1670, 2859, 3708
SacI GAGCTC 3 cut(s) 103, 1417, 3740
SapI GCTCTTC 1 cut(s) 1422
Sau96I GGNCC 6 cut(s) 283, 1390, 1862, 3726, 3926, 4220
SchI GAGTC 2 cut(s) 1915, 4372
ScrFI CCNGG 8 cut(s) 502, 1239, 1384, 1679, 2240, 2937, 3365, 3366
SduI GDGCHC 4 cut(s) 103, 1417, 3740, 3772
SfcI CTRYAG 6 cut(s) 146, 1494, 1569, 1778, 2269, 2655
SinI GGWCC 6 cut(s) 283, 1390, 1862, 3726, 3926, 4220
SmaI CCCGGG 1 cut(s) 3366
SmiMI CAYNNNNRTG 5 cut(s) 597, 848, 1670, 2859, 3708
SnaBI TACGTA 1 cut(s) 4006
SstI GAGCTC 3 cut(s) 103, 1417, 3740
StyD4I CCNGG 8 cut(s) 500, 1237, 1382, 1677, 2238, 2935, 3363, 3364
StyI CCWWGG 4 cut(s) 2410, 3061, 3218, 3327
TaaI ACNGT 9 cut(s) 731, 780, 1782, 2129, 2178, 2785, 2799, 3131, 3167
TaiI ACGT 5 cut(s) 790, 2449, 3262, 4008, 4338
TatI WGTACW 4 cut(s) 775, 898, 1123, 2534
TauI GCSGC 6 cut(s) 669, 1568, 1842, 2467, 2495, 3177
TscAI CASTG 9 cut(s) 734, 1787, 2005, 2132, 2181, 2187, 3274, 3529, 3909
TseFI GTSAC 4 cut(s) 2024, 3260, 3527, 4336
TseI GCWGC 6 cut(s) 409, 449, 2657, 2703, 3492, 4032
Tsp45I GTSAC 4 cut(s) 2024, 3260, 3527, 4336
TspGWI ACGGA 5 cut(s) 1166, 2273, 2971, 3818, 4041
TspMI CCCGGG 1 cut(s) 3364
TspRI CASTG 9 cut(s) 734, 1787, 2005, 2132, 2181, 2187, 3274, 3529, 3909
Tth111I GACNNNGTC 1 cut(s) 836
Vha464I CTTAAG 1 cut(s) 2069
VpaK11BI GGWCC 6 cut(s) 283, 1390, 1862, 3726, 3926, 4220
VspI ATTAAT 1 cut(s) 3237
XapI RAATTY 8 cut(s) 791, 1558, 2088, 2946, 3227, 3278, 4286, 4372
XceI RCATGY 5 cut(s) 995, 1320, 1535, 1831, 4110
XcmI CCANNNNNNNNNTGG 1 cut(s) 2668
XmaI CCCGGG 1 cut(s) 3364
XmaJI CCTAGG 1 cut(s) 2410
XmiI GTMKAC 4 cut(s) 126, 840, 1141, 2406
XmnI GAANNNNTTC 3 cut(s) 1055, 1070, 2427
Zsp2I ATGCAT 1 cut(s) 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.