Rroxscaffold_4G00282480

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000067
Physical Location & Seq
Reverse (-)
4534831 .. 4541545
6715 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00282480.1

Sequence Viewer

Length: 3969 bp
ATGGCCGCTTTTTCTTCTTCCTCTTCTTCTTCTTCTTCTTCTTCCTCTTCCTCTGTCGGTCGCTGGAACTACGATGTCTTCATCAGTTTTAGAGGCGATGACACTCGCAAGAACTTTGTCGGCCATCTCTACAGAGCTCTCCGTCAGAAAGCAATCAACACCTTCATCGATTCCGACGACCTTCGAACAGGAAACGACCCTTCAGAGCTTCTGGAAGCCATCGCCGACTCGAGGCTTTCCATTGTGGTGTTCTCCAAACACTATGCTTCTTCCAGGTGGTGCCTCAAAGAGCTGGTCAAGATCGTGGAGTGCATGAAAACGATGAAGCAAATTGTGGTGCCCGTTTTCTACGAACTTGATGCTTCTGATATTCGGAAACCCAAAGCGAGTTTTGAGCAAGCTTTTGATGAACATACGCATGTGGAAGAGGTGCAGAGGTGGAAGTCTGCTCTAGTTGAGGCCTCCAATTTATCGGGCTGGGATTCAAGAAATTATGAGGATGACGCCAAGCTTATTGAGAAAATAGTCGGTGATATTTCTAAAAGATTGATCCGCGTCTCATCCAGTAAAGACAATGTTTTGGTTGGAATGGATAAACACATTGCAGAAATAGATGTACTATTGTGTCTTGGGGTGGATGATGTTCGTGGTATTGGAATATTCGGAATGTCTGGTATAGGTAAAACAACCATAGCTCGAGCTGTTTATGATGAAATCACTTGTCAGTTTGAACACTATTGCTTTCTTGAAAATGTCAAAGATGGTTTCAAGAATAAAGGTGCAGTACATATGCAGGAAGAACTTCTATCTAGAATCTTGAAGAAAAAGGTGTGTACCTTAGGTAATTTGAATACAGGCTTCAAGATGGTTATGGAAAGGCTGAATAAGAAAAGAGTTTTACTTGTGCTTGATGATGTCGAAAATTTTGCCCAAATTGAAGCCTTACTCGGAAAGCAATGTTCATTTGGTGGTGGAAGTAGAATCATTATAACAACTAAAGATATGCAATCACTAAGTGGAGTGCAGGAGAGATATAGTCCCAAATTTTTAAGTGATGATGAAGATCTTGAGCTTTTTATGCAGTATGCCTTCAGAACAAACCAACCTGCAGGAGAGTACGATTCTCTCTCAAGGCGTGCCATAGAATATGCTCAAGGTCTGCCTTTAGCACTTAAAGTTTTGGGAGCTTTTCTTGATAGCAAAAGTGAATGTGAGTGGGAAGATGAGCTAGAAAAATTAAACAAAATCCCACACATAGAAATTCAGGGTGTGCTTAGAACAAGCTTTGATGGACTAGATCCTTTACAAAAGGATATATTTCTAGATATTGCATGCTTCTTCAGAGGAATGGACAAAGGATATGTAACAAAAGTTCTGGAAAGTTGTGGCTTTTACCCCCATAGTGGATTAAGAGTACTACTCGATAGAGCTCTCATCACAATCTCAGATGACAATAGGCTTGAAATGCATGATTTACTACAAGAAATAGGTTGGGAAATCGTCCGACGACAATCAATTAAAGAACCTGGGAAGCGCAGTAGGTTGTGGGTTTTTGAAGATGTCAATCATGTGTTCACTCATAATACGGCTACAGATGCAGTTGAGGGCATAATGCTTGACTTGTCAAGATCAAAAGAGGATTTATGTCATCCAAGTGATGTCTGTCAACAACATCTGAGTGGGAACTTCACGTTTCTTTCTAATGAGTTGAGGATTCTCCACTGGCATGGATTCCCCTTAAAGTCTTTGCCCTCCAATTTTGTGCCGACGAATCTTGTTGAACTTGACATGCGTTCTAGTCACATTGAACAACTGTGGGAAGGAATCAAGCCTCTGAAAAAGTTGAAAATTATTAATCTAAGTCACTCTCAATACCTTAAGAAAATCCCCGACTTCACTGGGGCAACGAATATGAAGAAACTAATTCTTGATGGTTGTTCAAGTTTATTGGAGGTTCATCCGTCAATTTCAGCTCTTAAAAACCTTGTTGTCATGAGTCTAAAAGGGTGTAAAGCAGTTAAGAGTCTTCCAGGTAGCATTTACATGAAATCTCTTAAAACCCTTGATCTTTCTGGCTGCTCAAATCTTAAGATGTTTCCAGAGATCTCAGGAATTATGGATGACCTATCGGAGCTTTATTTAAGTGAGACTGCAATTGAAGAACTACCGTCATCTATTGAACAGCTTCAGGGGCTTGTGTTATTGAATATGGCAGACTGCAGAAGCCTTGTCTGTCTTCCAGACAATATCTGTAATTTGGCATACCTTACAAATCTCGCTCTCTCTGGGTGCTCAAAACTTTATCGTTTGCCTGAGAACTTGGGGAATTTAGAATCCTTGATGGTCCTTGAAATAGAAAGAAGTGGTATAAAAGAACTCCCTTTTTCGATCTTACATGTGAATAAGCTCAAAAGATTATCATGTGATGGATGTAAAGAAATGATGGCGCCCCTTTCATCATGGTCATCATCAATCAAAGGATATTATACTTACTCTGGTATGCTACATCTTGATTTAAGTGACTGCAATCTGATGGTATTATCAGATGGTATTGCTCATTTGTCCTCATTAAGAACGTTAGAGCTGCGCAGAAACAACTTGGAGAGCTTACCTGCAGCAATGAATCGACTTCGTTGTTTAACACATCTTGAATTAGAAGCGTGCAAGAAACTGAAATCAATACCAGAGCTTTCATCAAGTATTAGTTACATAGATGCACATGATTGCACAGCTTTGGAAACAGTTTCAACACCAAAGCCTCGCTCTTCTACAAATCTCTGCTTCACATTTTCAAATTGTCTCCAGCTGATACAAACAAATCTATTCAGGGATATTGTGGAAACTCACTCACATCATCAGGGTAGCTATCTACGACCTCTTTCCTTTAGTATGTCACTTCCTGGAAGTGAAGTTCCTCATTGGTTCAACCATCAGTGTAAGGGATTCTCAGTGACTGTGCAGCTACCACAGAATTGGTTCGATGATAAGTTTTTGGGATTTGCTATATGCGGTGTTAGTAACTTCAAGGGTGCTCATAATGATGCGTCCGATCTATCTGCTGTTTGTTTTTGTACCTTCAAAGGAAATAACGGTGAGTACAAGTTCAGTTTTCATTTGCTTGATTGGGGTTTTAGAACAAACAGATACCTCCAATCAGATCACATGTTCCTGGGATATGTGCCATGGTCACAATATCGCTTCAGTGATGAAGGGAAGCAGGTCAATGAACGCTACTACACTGAAGTCAGATTTGAGATTGTGGTAGAAAATGGAGTCTATGCACATGATGATTACAGGACAGTAATACGACGACATTGCATCACAAGTTGTGGAGTGCGTTTGTTTCATGGTAATTTTATGGGACTGCAAAATCTAAGCATCACTCAGCCTAATTCTCATCATGATGGCTGTGGAATCCCATTGGACACTGCGAGGAAGAGAAAGAGAGATATAGCAGGGGTGACATTGCAGTGGGAGTGGCCTCCCACAGAAGAGGTGTTCACTGAGCAAGAGCAAGAAGGATCCCCATCAAGGGAAGGGTCCCTGGCGGGGGAAGGGTCCTCTGCGGGCAAGAGGTCCTCGGCGGGGGAAGGGGTTCCTTCTATTCCTACTGCAGTAGCAACAGTAGAAAGTGGTGCCAATGAACGTGATGATCCAACTATCCCTAATCGTACAAACAGAAGAAGGCGCACAAATTTGCGAAATTCAGCAACGTGGATCCGAGATACAAATCAACTTATTGATGACGGCCATGCTTGGAGAAAGTATGGCCAAAAGGAGATACTTGGATCCAGATATCCCAGAAACTATTACAGATGCACTTATCGAATGGATCAAGGCTGTATGGCCACTAAGCACGTGCAACGCATCAGTGACGAGCCAGTATTATATAGAACTACTTACAGAGGAAATCACACATGCAGACCCGTCTCCAGCATACAGGGCATAGAAGAGTATTTGGCATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1322

Amino Acids

149.51

Weight (kDa)

6.45

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 195 3.3e-52 TIR domain
TIR_2 PF13676 26 - 135 4.1e-16 TIR domain
NB-ARC PF00931 212 - 367 1.1e-15 NB-ARC domain
WHD_ROQ1 PF23282 436 - 505 4.9e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 591 - 610 5.8e-07 Leucine Rich Repeat
LRR_14 PF23598 651 - 762 1.2e-08 Leucine-rich repeat region
LRR_13 PF23286 682 - 767 6.6e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 749 - 814 4.7e-09 Leucine-rich repeat region
LRR_13 PF23286 769 - 917 2.8e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 836 - 897 2.1e-06 Leucine-rich repeat region
LRR_8 PF13855 836 - 888 7.4e-07 Leucine rich repeat
C-JID PF20160 966 - 1116 6.2e-21 C-JID domain
WRKY PF03106 1248 - 1307 6.8e-24 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 989
Acc16I TGCGCA 1 cut(s) 2588
Acc36I ACCTGC 3 cut(s) 1114, 2620, 3208
AccB1I GGYRCC 4 cut(s) 279, 337, 2446, 3635
AccII CGCG 1 cut(s) 555
AciI CCGC 6 cut(s) 6, 553, 3009, 3548, 3566, 3584
AclI AACGTT 1 cut(s) 2576
AcoI YGGCCR 5 cut(s) 3, 121, 3748, 3769, 3846
AcsI RAATTY 6 cut(s) 922, 1043, 1260, 2326, 3694, 3703
AcuI CTGAAG 6 cut(s) 186, 1075, 1324, 2171, 3184, 3261
AcvI CACGTG 1 cut(s) 3859
AcyI GRCGYC 2 cut(s) 504, 2447
AdeI CACNNNGTG 1 cut(s) 1016
AfaI GTAC 8 cut(s) 618, 786, 835, 1118, 1416, 3073, 3098, 3673
AfiI CCNNNNNNNGG 7 cut(s) 231, 1403, 3531, 3532, 3549, 3550, 3585
AflII CTTAAG 2 cut(s) 1877, 2087
AflIII ACRYGT 2 cut(s) 2395, 3162
AjnI CCWGG 6 cut(s) 272, 1525, 2029, 2899, 3168, 3543
AloI GAACNNNNNNTCC 2 cut(s) 2895, 2927
Alw21I GWGCWC 4 cut(s) 139, 1432, 2294, 3034
Alw26I GTCTC 4 cut(s) 562, 2141, 2804, 3934
AlwNI CAGNNNCTG 1 cut(s) 2954
Ama87I CYCGRG 2 cut(s) 229, 696
AoxI GGCC 7 cut(s) 3, 121, 459, 3479, 3748, 3769, 3846
ApeKI GCWGC 4 cut(s) 2076, 2584, 2615, 2959
ApoI RAATTY 6 cut(s) 922, 1043, 1260, 2326, 3694, 3703
ArsI GACNNNNNNTTYG 2 cut(s) 279, 311
AseI ATTAAT 1 cut(s) 1854
Asp700I GAANNNNTTC 4 cut(s) 801, 2184, 2975, 3594
AspLEI GCGC 4 cut(s) 1536, 2449, 2589, 3690
AspS9I GGNCC 4 cut(s) 2344, 3540, 3558, 3576
AsuHPI GGTGA 3 cut(s) 542, 3104, 3472
AsuII TTCGAA 1 cut(s) 184
AvaI CYCGRG 2 cut(s) 229, 696
AvaII GGWCC 4 cut(s) 2344, 3540, 3558, 3576
AxyI CCTNAGG 1 cut(s) 838
BaeGI GKGCMC 1 cut(s) 342
BalI TGGCCA 2 cut(s) 3771, 3848
BamHI GGATCC 3 cut(s) 3521, 3717, 3788
BanI GGYRCC 4 cut(s) 279, 337, 2446, 3635
BanII GRGCYC 2 cut(s) 139, 1432
BbrPI CACGTG 1 cut(s) 3859
BbsI GAAGAC 3 cut(s) 70, 2018, 2228
Bbv12I GWGCWC 4 cut(s) 139, 1432, 2294, 3034
BbvI GCAGC 4 cut(s) 2063, 2571, 2627, 2971
BceAI ACGGC 2 cut(s) 1602, 3763
BcgI CGANNNNNNTGC 8 cut(s) 341, 375, 908, 942, 3038, 3072, 3297, 3331
BciT130I CCWGG 6 cut(s) 274, 1527, 2031, 2901, 3170, 3545
BcoDI GTCTC 4 cut(s) 562, 2141, 2804, 3934
BfaI CTAG 6 cut(s) 452, 810, 1229, 1295, 1322, 1797
BfmI CTRYAG 6 cut(s) 130, 1107, 1590, 2218, 2613, 3612
BfoI RGCGCY 1 cut(s) 2450
BfrI CTTAAG 2 cut(s) 1877, 2087
BfuAI ACCTGC 3 cut(s) 1114, 2620, 3208
BglII AGATCT 2 cut(s) 1063, 2103
BisI GCNGC 5 cut(s) 6, 2077, 2585, 2616, 2960
BlsI GCNGC 5 cut(s) 7, 2078, 2586, 2617, 2961
BmcAI AGTACT 1 cut(s) 1416
Bme1390I CCNGG 6 cut(s) 274, 1527, 2031, 2901, 3170, 3545
Bme18I GGWCC 4 cut(s) 2344, 3540, 3558, 3576
BmeT110I CYCGRG 2 cut(s) 229, 696
BmgT120I GGNCC 4 cut(s) 2344, 3540, 3558, 3576
BmrFI CCNGG 6 cut(s) 274, 1527, 2031, 2901, 3170, 3545
BmrI ACTGGG 1 cut(s) 1908
BmsI GCATC 8 cut(s) 349, 1585, 2704, 3031, 3327, 3387, 3806, 3876
BmuI ACTGGG 1 cut(s) 1908
BpiI GAAGAC 3 cut(s) 70, 2018, 2228
BplI GAGNNNNNCTC 2 cut(s) 1404, 1436
BpmI CTGGAG 2 cut(s) 2786, 3916
Bpu14I TTCGAA 1 cut(s) 184
BpuEI CTTGAG 3 cut(s) 1088, 1114, 1137
Bsa29I ATCGAT 1 cut(s) 168
BsaAI YACGTR 1 cut(s) 3859
BsaBI GATNNNNATC 4 cut(s) 1062, 1563, 3526, 3729
BsaHI GRCGYC 2 cut(s) 504, 2447
BsaJI CCNNGG 5 cut(s) 1526, 3169, 3182, 3543, 3579
Bsc4I CCNNNNNNNGG 7 cut(s) 231, 1403, 3531, 3532, 3549, 3550, 3585
Bse1I ACTGG 4 cut(s) 564, 1727, 1903, 3881
Bse21I CCTNAGG 1 cut(s) 838
Bse3DI GCAATG 5 cut(s) 600, 962, 2625, 3313, 3464
Bse8I GATNNNNATC 4 cut(s) 1062, 1563, 3526, 3729
BseBI CCWGG 6 cut(s) 274, 1527, 2031, 2901, 3170, 3545
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 5 cut(s) 1526, 3169, 3182, 3543, 3579
BseGI GGATG 7 cut(s) 505, 560, 643, 1648, 1957, 2125, 2435
BseJI GATNNNNATC 4 cut(s) 1062, 1563, 3526, 3729
BseLI CCNNNNNNNGG 7 cut(s) 231, 1403, 3531, 3532, 3549, 3550, 3585
BseMI GCAATG 5 cut(s) 600, 962, 2625, 3313, 3464
BseMII CTCAG 7 cut(s) 1458, 1667, 2121, 2304, 2961, 3398, 3495
BseNI ACTGG 4 cut(s) 564, 1727, 1903, 3881
BseSI GKGCMC 1 cut(s) 342
BseXI GCAGC 4 cut(s) 2063, 2571, 2627, 2971
BseYI CCCAGC 1 cut(s) 477
BsgI GTGCAG 4 cut(s) 452, 801, 1043, 2978
Bsh1236I CGCG 1 cut(s) 555
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 7 cut(s) 5, 123, 461, 3481, 3750, 3771, 3848
BshNI GGYRCC 4 cut(s) 279, 337, 2446, 3635
BshVI ATCGAT 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 4 cut(s) 139, 1432, 2294, 3034
BsiHKCI CYCGRG 2 cut(s) 229, 696
BslFI GGGAC 3 cut(s) 1023, 3375, 3526
BslI CCNNNNNNNGG 7 cut(s) 231, 1403, 3531, 3532, 3549, 3550, 3585
BsmAI GTCTC 4 cut(s) 562, 2141, 2804, 3934
BsmBI CGTCTC 2 cut(s) 562, 3934
BsmFI GGGAC 3 cut(s) 1023, 3375, 3526
BsnI GGCC 7 cut(s) 5, 123, 461, 3481, 3750, 3771, 3848
BsoBI CYCGRG 2 cut(s) 229, 696
Bsp119I TTCGAA 1 cut(s) 184
Bsp1286I GDGCHC 5 cut(s) 139, 342, 1432, 2294, 3034
Bsp19I CCATGG 1 cut(s) 3182
BspACI CCGC 6 cut(s) 6, 553, 3009, 3548, 3566, 3584
BspANI GGCC 7 cut(s) 5, 123, 461, 3481, 3750, 3771, 3848
BspCNI CTCAG 7 cut(s) 1457, 1668, 2120, 2305, 2960, 3397, 3496
BspDI ATCGAT 1 cut(s) 168
BspFNI CGCG 1 cut(s) 555
BspHI TCATGA 2 cut(s) 1992, 3400
BspMAI CTGCAG 4 cut(s) 1111, 2222, 2617, 3616
BspMI ACCTGC 3 cut(s) 1114, 2620, 3208
BspQI GCTCTTC 1 cut(s) 2770
BspT104I TTCGAA 1 cut(s) 184
BspT107I GGYRCC 4 cut(s) 279, 337, 2446, 3635
BspTI CTTAAG 2 cut(s) 1877, 2087
BsrDI GCAATG 5 cut(s) 600, 962, 2625, 3313, 3464
BsrI ACTGG 4 cut(s) 564, 1727, 1903, 3881
BssECI CCNNGG 5 cut(s) 1526, 3169, 3182, 3543, 3579
BssNI GRCGYC 2 cut(s) 504, 2447
BssT1I CCWWGG 1 cut(s) 3182
Bst2UI CCWGG 6 cut(s) 274, 1527, 2031, 2901, 3170, 3545
Bst4CI ACNGT 7 cut(s) 1815, 2169, 2743, 2956, 3092, 3301, 3625
Bst6I CTCTTC 7 cut(s) 28, 52, 420, 2770, 3431, 3486, 3945
BstACI GRCGYC 2 cut(s) 504, 2447
BstAFI CTTAAG 2 cut(s) 1877, 2087
BstBAI YACGTR 1 cut(s) 3859
BstBI TTCGAA 1 cut(s) 184
BstC8I GCNNGC 5 cut(s) 399, 1137, 1333, 2662, 3568
BstDSI CCRYGG 1 cut(s) 3182
BstF5I GGATG 7 cut(s) 505, 560, 643, 1648, 1957, 2125, 2435
BstFNI CGCG 1 cut(s) 555
BstH2I RGCGCY 1 cut(s) 2450
BstHHI GCGC 4 cut(s) 1536, 2449, 2589, 3690
BstMAI GTCTC 4 cut(s) 562, 2141, 2804, 3934
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 5 cut(s) 1078, 1465, 1595, 2191, 3942
BstNI CCWGG 6 cut(s) 274, 1527, 2031, 2901, 3170, 3545
BstNSI RCATGY 6 cut(s) 422, 1335, 1792, 2399, 3166, 3921
BstSCI CCNGG 6 cut(s) 272, 1525, 2029, 2899, 3168, 3543
BstSFI CTRYAG 6 cut(s) 130, 1107, 1590, 2218, 2613, 3612
BstSLI GKGCMC 1 cut(s) 342
BstUI CGCG 1 cut(s) 555
BstV1I GCAGC 4 cut(s) 2063, 2571, 2627, 2971
BstV2I GAAGAC 3 cut(s) 70, 2018, 2228
BstX2I RGATCY 6 cut(s) 1063, 1297, 2103, 3521, 3717, 3788
BstXI CCANNNNNNTGG 2 cut(s) 1727, 2973
BstYI RGATCY 6 cut(s) 1063, 1297, 2103, 3521, 3717, 3788
Bsu15I ATCGAT 1 cut(s) 168
Bsu36I CCTNAGG 1 cut(s) 838
BsuRI GGCC 7 cut(s) 5, 123, 461, 3481, 3750, 3771, 3848
BsuTUI ATCGAT 1 cut(s) 168
BtgI CCRYGG 1 cut(s) 3182
BtgZI GCGATG 2 cut(s) 111, 205
BtsCI GGATG 7 cut(s) 505, 560, 643, 1648, 1957, 2125, 2435
BtsI GCAGTG 2 cut(s) 3426, 3476
BveI ACCTGC 3 cut(s) 1114, 2620, 3208
Cac8I GCNNGC 5 cut(s) 399, 1137, 1333, 2662, 3568
CaiI CAGNNNCTG 1 cut(s) 2954
CciI TCATGA 2 cut(s) 1992, 3400
CfoI GCGC 4 cut(s) 1536, 2449, 2589, 3690
Cfr13I GGNCC 4 cut(s) 2344, 3540, 3558, 3576
ClaI ATCGAT 1 cut(s) 168
CseI GACGC 3 cut(s) 512, 544, 3033
Csp6I GTAC 8 cut(s) 617, 785, 834, 1117, 1415, 3072, 3097, 3672
CviQI GTAC 8 cut(s) 617, 785, 834, 1117, 1415, 3072, 3097, 3672
DinI GGCGCC 1 cut(s) 2448
DraIII CACNNNGTG 1 cut(s) 1016
EaeI YGGCCR 5 cut(s) 3, 121, 3748, 3769, 3846
Eam1104I CTCTTC 7 cut(s) 28, 52, 420, 2770, 3431, 3486, 3945
EarI CTCTTC 7 cut(s) 28, 52, 420, 2770, 3431, 3486, 3945
Ecl136II GAGCTC 2 cut(s) 137, 1430
Eco130I CCWWGG 1 cut(s) 3182
Eco147I AGGCCT 1 cut(s) 461
Eco24I GRGCYC 2 cut(s) 139, 1432
Eco32I GATATC 1 cut(s) 3797
Eco47I GGWCC 4 cut(s) 2344, 3540, 3558, 3576
Eco53kI GAGCTC 2 cut(s) 137, 1430
Eco57I CTGAAG 6 cut(s) 186, 1075, 1324, 2171, 3184, 3261
Eco72I CACGTG 1 cut(s) 3859
Eco81I CCTNAGG 1 cut(s) 838
Eco88I CYCGRG 2 cut(s) 229, 696
EcoICRI GAGCTC 2 cut(s) 137, 1430
EcoO109I RGGNCCY 3 cut(s) 3540, 3558, 3576
EcoRII CCWGG 6 cut(s) 272, 1525, 2029, 2899, 3168, 3543
EcoRV GATATC 1 cut(s) 3797
EcoT14I CCWWGG 1 cut(s) 3182
EcoT22I ATGCAT 1 cut(s) 1470
EcoT38I GRGCYC 2 cut(s) 139, 1432
EgeI GGCGCC 1 cut(s) 2448
EheI GGCGCC 1 cut(s) 2448
ErhI CCWWGG 1 cut(s) 3182
Esp3I CGTCTC 2 cut(s) 562, 3934
FaqI GGGAC 3 cut(s) 1023, 3375, 3526
FauI CCCGC 3 cut(s) 3541, 3559, 3577
FauNDI CATATG 1 cut(s) 789
Fnu4HI GCNGC 5 cut(s) 6, 2077, 2585, 2616, 2960
FokI GGATG 7 cut(s) 512, 547, 650, 1635, 1944, 2132, 2442
FriOI GRGCYC 2 cut(s) 139, 1432
Fsp4HI GCNGC 5 cut(s) 6, 2077, 2585, 2616, 2960
FspBI CTAG 6 cut(s) 452, 810, 1229, 1295, 1322, 1797
FspI TGCGCA 1 cut(s) 2588
GlaI GCGC 4 cut(s) 1535, 2448, 2588, 3689
GluI GCNGC 5 cut(s) 6, 2077, 2585, 2616, 2960
GsaI CCCAGC 1 cut(s) 481
GsuI CTGGAG 2 cut(s) 2786, 3916
HaeII RGCGCY 1 cut(s) 2450
HaeIII GGCC 7 cut(s) 5, 123, 461, 3481, 3750, 3771, 3848
HgaI GACGC 3 cut(s) 512, 544, 3033
HhaI GCGC 4 cut(s) 1536, 2449, 2589, 3690
Hin1I GRCGYC 2 cut(s) 504, 2447
Hin6I GCGC 4 cut(s) 1534, 2447, 2587, 3688
HinP1I GCGC 4 cut(s) 1534, 2447, 2587, 3688
HincII GTYRAC 1 cut(s) 1667
HindII GTYRAC 1 cut(s) 1667
HindIII AAGCTT 3 cut(s) 399, 509, 1282
HphI GGTGA 3 cut(s) 542, 3104, 3472
Hpy166II GTNNAC 4 cut(s) 834, 1575, 1667, 3501
Hpy8I GTNNAC 4 cut(s) 834, 1575, 1667, 3501
Hpy99I CGWCG 4 cut(s) 179, 1509, 1771, 3312
HpyCH4III ACNGT 7 cut(s) 1815, 2169, 2743, 2956, 3092, 3301, 3625
HpyCH4IV ACGT 5 cut(s) 1691, 2576, 3646, 3713, 3858
HpyF10VI GCNNNNNNNGC 5 cut(s) 1078, 1465, 1595, 2191, 3942
HpySE526I ACGT 5 cut(s) 1691, 2576, 3646, 3713, 3858
Hsp92I GRCGYC 2 cut(s) 504, 2447
HspAI GCGC 4 cut(s) 1534, 2447, 2587, 3688
KasI GGCGCC 1 cut(s) 2446
KflI GGGWCCC 1 cut(s) 3540
LguI GCTCTTC 1 cut(s) 2770
LmnI GCTCC 2 cut(s) 1184, 2131
Lsp1109I GCAGC 4 cut(s) 2063, 2571, 2627, 2971
LweI GCATC 8 cut(s) 349, 1585, 2704, 3031, 3327, 3387, 3806, 3876
MaeI CTAG 6 cut(s) 452, 810, 1229, 1295, 1322, 1797
MaeII ACGT 5 cut(s) 1691, 2576, 3646, 3713, 3858
MfeI CAATTG 1 cut(s) 2154
MflI RGATCY 6 cut(s) 1063, 1297, 2103, 3521, 3717, 3788
MhlI GDGCHC 5 cut(s) 139, 342, 1432, 2294, 3034
MlsI TGGCCA 2 cut(s) 3771, 3848
MluNI TGGCCA 2 cut(s) 3771, 3848
Mly113I GGCGCC 1 cut(s) 2447
MlyI GAGTC 4 cut(s) 221, 2005, 2032, 3282
MmeI TCCRAC 4 cut(s) 198, 565, 1528, 3680
Mox20I TGGCCA 2 cut(s) 3771, 3848
Mph1103I ATGCAT 1 cut(s) 1470
MroXI GAANNNNTTC 4 cut(s) 801, 2184, 2975, 3594
MscI TGGCCA 2 cut(s) 3771, 3848
MslI CAYNNNNRTG 9 cut(s) 245, 417, 1653, 1677, 1725, 2042, 3039, 3402, 3469
Msp20I TGGCCA 2 cut(s) 3771, 3848
MspA1I CMGCKG 1 cut(s) 2806
MspCI CTTAAG 2 cut(s) 1877, 2087
MspR9I CCNGG 6 cut(s) 274, 1527, 2031, 2901, 3170, 3545
MunI CAATTG 1 cut(s) 2154
MvaI CCWGG 6 cut(s) 274, 1527, 2031, 2901, 3170, 3545
MvnI CGCG 1 cut(s) 555
MwoI GCNNNNNNNGC 5 cut(s) 1078, 1465, 1595, 2191, 3942
NarI GGCGCC 1 cut(s) 2447
NcoI CCATGG 1 cut(s) 3182
NdeI CATATG 1 cut(s) 789
NmeAIII GCCGAG 1 cut(s) 3560
NmuCI GTSAC 8 cut(s) 1799, 1862, 2519, 2892, 2950, 3186, 3460, 3872
NsbI TGCGCA 1 cut(s) 2588
NsiI ATGCAT 1 cut(s) 1470
NspI RCATGY 6 cut(s) 422, 1335, 1792, 2399, 3166, 3921
NspV TTCGAA 1 cut(s) 184
PaeI GCATGC 1 cut(s) 1335
PaeR7I CTCGAG 2 cut(s) 229, 696
PagI TCATGA 2 cut(s) 1992, 3400
PceI AGGCCT 1 cut(s) 461
PciI ACATGT 2 cut(s) 2395, 3162
PciSI GCTCTTC 1 cut(s) 2770
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PdmI GAANNNNTTC 4 cut(s) 801, 2184, 2975, 3594
PfoI TCCNGGA 1 cut(s) 2899
PkrI GCNGC 5 cut(s) 7, 2078, 2586, 2617, 2961
PleI GAGTC 4 cut(s) 221, 2004, 2031, 3281
PluTI GGCGCC 1 cut(s) 2450
PmaCI CACGTG 1 cut(s) 3859
PmlI CACGTG 1 cut(s) 3859
PpsI GAGTC 4 cut(s) 221, 2004, 2031, 3281
Ppu21I YACGTR 1 cut(s) 3859
PpuMI RGGWCCY 3 cut(s) 3540, 3558, 3576
PscI ACATGT 2 cut(s) 2395, 3162
PshBI ATTAAT 1 cut(s) 1854
PsiI TTATAA 1 cut(s) 989
Psp124BI GAGCTC 2 cut(s) 139, 1432
Psp1406I AACGTT 1 cut(s) 2576
Psp5II RGGWCCY 3 cut(s) 3540, 3558, 3576
Psp6I CCWGG 6 cut(s) 272, 1525, 2029, 2899, 3168, 3543
PspCI CACGTG 1 cut(s) 3859
PspFI CCCAGC 1 cut(s) 477
PspGI CCWGG 6 cut(s) 272, 1525, 2029, 2899, 3168, 3543
PspPI GGNCC 4 cut(s) 2344, 3540, 3558, 3576
PspPPI RGGWCCY 3 cut(s) 3540, 3558, 3576
PspXI VCTCGAGB 2 cut(s) 229, 696
PsrI GAACNNNNNNTAC 2 cut(s) 1356, 1388
PstI CTGCAG 4 cut(s) 1111, 2222, 2617, 3616
PstNI CAGNNNCTG 1 cut(s) 2954
PsuI RGATCY 6 cut(s) 1063, 1297, 2103, 3521, 3717, 3788
PvuII CAGCTG 1 cut(s) 2806
RsaI GTAC 8 cut(s) 618, 786, 835, 1118, 1416, 3073, 3098, 3673
RsaNI GTAC 8 cut(s) 617, 785, 834, 1117, 1415, 3072, 3097, 3672
RseI CAYNNNNRTG 9 cut(s) 245, 417, 1653, 1677, 1725, 2042, 3039, 3402, 3469
SacI GAGCTC 2 cut(s) 139, 1432
SapI GCTCTTC 1 cut(s) 2770
SatI GCNGC 5 cut(s) 6, 2077, 2585, 2616, 2960
Sau96I GGNCC 4 cut(s) 2344, 3540, 3558, 3576
SbfI CCTGCAGG 1 cut(s) 1111
ScaI AGTACT 1 cut(s) 1416
SchI GAGTC 4 cut(s) 221, 2005, 2032, 3282
ScrFI CCNGG 6 cut(s) 274, 1527, 2031, 2901, 3170, 3545
SdaI CCTGCAGG 1 cut(s) 1111
SduI GDGCHC 5 cut(s) 139, 342, 1432, 2294, 3034
SfaNI GCATC 8 cut(s) 349, 1585, 2704, 3031, 3327, 3387, 3806, 3876
SfcI CTRYAG 6 cut(s) 130, 1107, 1590, 2218, 2613, 3612
SfoI GGCGCC 1 cut(s) 2448
Sfr274I CTCGAG 2 cut(s) 229, 696
SfuI TTCGAA 1 cut(s) 184
SinI GGWCC 4 cut(s) 2344, 3540, 3558, 3576
SlaI CTCGAG 2 cut(s) 229, 696
SmiMI CAYNNNNRTG 9 cut(s) 245, 417, 1653, 1677, 1725, 2042, 3039, 3402, 3469
SmlI CTYRAG 7 cut(s) 229, 696, 1067, 1129, 1152, 1877, 2087
SmoI CTYRAG 7 cut(s) 229, 696, 1067, 1129, 1152, 1877, 2087
SphI GCATGC 1 cut(s) 1335
Sse8387I CCTGCAGG 1 cut(s) 1111
SseBI AGGCCT 1 cut(s) 461
SsiI CCGC 6 cut(s) 6, 553, 3009, 3548, 3566, 3584
SspDI GGCGCC 1 cut(s) 2446
SspI AATATT 1 cut(s) 660
SspMI CTAG 6 cut(s) 452, 810, 1229, 1295, 1322, 1797
SstI GAGCTC 2 cut(s) 139, 1432
StuI AGGCCT 1 cut(s) 461
StyD4I CCNGG 6 cut(s) 272, 1525, 2029, 2899, 3168, 3543
StyI CCWWGG 1 cut(s) 3182
TaaI ACNGT 7 cut(s) 1815, 2169, 2743, 2956, 3092, 3301, 3625
TaiI ACGT 5 cut(s) 1694, 2579, 3649, 3716, 3861
TaqII GACCGA 1 cut(s) 47
TatI WGTACW 4 cut(s) 616, 784, 1414, 3096
TauI GCSGC 1 cut(s) 8
TseFI GTSAC 8 cut(s) 1799, 1862, 2519, 2892, 2950, 3186, 3460, 3872
TseI GCWGC 4 cut(s) 2076, 2584, 2615, 2959
Tsp45I GTSAC 8 cut(s) 1799, 1862, 2519, 2892, 2950, 3186, 3460, 3872
TspGWI ACGGA 2 cut(s) 131, 1950
Vha464I CTTAAG 2 cut(s) 1877, 2087
VpaK11BI GGWCC 4 cut(s) 2344, 3540, 3558, 3576
VspI ATTAAT 1 cut(s) 1854
XapI RAATTY 6 cut(s) 922, 1043, 1260, 2326, 3694, 3703
XbaI TCTAGA 2 cut(s) 809, 1321
XceI RCATGY 6 cut(s) 422, 1335, 1792, 2399, 3166, 3921
XhoI CTCGAG 2 cut(s) 229, 696
XmnI GAANNNNTTC 4 cut(s) 801, 2184, 2975, 3594
XspI CTAG 6 cut(s) 452, 810, 1229, 1295, 1322, 1797
ZrmI AGTACT 1 cut(s) 1416
Zsp2I ATGCAT 1 cut(s) 1470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.