MD07G1245600.v1.1

RESISTANCE protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000067
Physical Location & Seq
Reverse (-)
31554098 .. 31557149
3052 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1245600.v1.1.491

Sequence Viewer

Length: 1650 bp
ATGGAGAAGGATATCTTTCTAGATATTGCATGTTACTTTAAAGGAATGAGAGAAGACTGTGCAACCGAAGTTCTGCACAGCTGTGGTTTCTATCCTGATATTGGAATAAAAGTTCTAATCGATCGAGCTCTAGTAACTGTCTCAACGAAGGGGGAACTGGAGATGCATGATTTACTAGAGGAAATGGGTCAGAAAATCGTCTACCAAGAATCTATCAAAGAGCCTGGGAGACGAAGTAGGTTGTGGAGTTATGAAGATGTTTTTCATGTGATAACTAAAAATACGGGTACAGAAACAGTCGAAAGCATAATCCTCGATTCGTGGAACTCAGATGAGGGATGCTTAATGGTTGAAGCTTTTGATAGGATGACTAAACTAAGACTTCTCAAGATCAGTGCTCACGGCATACAACACCTGACTGGGCCCTTAAAGTTTGTTTCTAGTGAGTTGAGGTGTCTTTCCTGGTCTGGATACCCTCTCAAGTCTTTGCCATCCAACTTTCAATTCGAAAATCTTGTTGGACTTGACATGCAATATAGCCTCATTGACCGACTTTGGGAAGGAACCCAGGAAAAGTTGAAATTCATCAATTTAAGTCATTGTCATAACCTTGAGATAACCCCTGACTTCACAAATGTGCCAAATCTTGAGAGGCTAATTGTTCAAGGTTGTACACGCTTAGTTGAGGTTCACGGGTCGATTTTGGCTCTTACAAAAATTGTTTTATTGGATCTGAGTTTGTGCGGGCAACTTAAGATTCTACCCAGCAAAGTTTGTATGAGATCTCTCAAAACCTTTGGTCTTTTTGGCTGCCGGAGTCTTGAGATGTTTCCGGAGATTTCAGAAGTGATTGAGGGGTTAGAAAAGCTTGATTTATCCGGGTCAAGAATTAAAGAACTGCCCCTGTCAATTAATAATCTCACGGGGTTGAGTCATTTGAACCTATCAAAATGCATGGAACTTAAGATTCTACCCAGCAAAGTTTGTATGAGATCTCTCAAAACCTTTGATCTTTCTGGCTGCTCCAGTCTTGAGATGTTTCCAGAGATTTCAGAAGTGATTGAGGGGTTAGAAGCGCTTGATTTATCCAGGTCAAAAATTAAAGAACTACCCCTGTCAATTAATAATCTCACGAGGTTGAGTTATTTGGACCTAAACAATTGCAAGGAACTTGAGAGTCTTCCAAGCAGCATTCGTATGAAATCTCTCAAAACCCTTGATCTTTCTGGTTGCTCGAGTCTTGTGATGTTTCCAGAGATTTCAGAAGTTATTGAGGGGTTAGAAGAGCTTGATTTATCTGGGTCAAAAATTAAAGAACTACCCCTGTCAATTAATAATCTCACGGGATTGAGGTGTTTGCTTATGATGAATTGCAAGGAACTTGAGAGTCTTCCAAGCAGCATTTGTCAACTCAAATCCTTTTACCGTCTCTCTCTTTACAATTGTACAAAATTTGAGGTGTTTCCAAGCATTGAAGAAAATATGGAAGGATTAAGAATGCTTTTCTTGGGTGGAACATCTATCAAAGAGCTTTCCCCCTGGATTGAACGCCTTACGGGGCTTCAGGTTTTAAGTCTGATAAATTGCAAAAGACTTGTACACATTCCTGACACTCTATGTAATTTGGCACGCCTTAGCATATACACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_3 LRR_5 LRR_8 NB-ARC TIR

Protein Analysis

550

Amino Acids

62.02

Weight (kDa)

5.52

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 2 - 71 1.2e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 261 - 342 3.4e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 331 - 400 5.2e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 336 - 414 3.1e-06 Leucine-rich repeat region
LRR_14 PF23598 392 - 501 5.2e-09 Leucine-rich repeat region
LRR_13 PF23286 401 - 471 5.8e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 201
AccIII TCCGGA 1 cut(s) 832
AciI CCGC 1 cut(s) 744
AclWI GGATC 1 cut(s) 738
AcsI RAATTY 2 cut(s) 581, 1451
AcuI CTGAAG 1 cut(s) 1547
AfaI GTAC 4 cut(s) 289, 673, 1447, 1599
AfeI AGCGCT 1 cut(s) 1077
AfiI CCNNNNNNNGG 2 cut(s) 101, 556
AflII CTTAAG 2 cut(s) 752, 962
AgsI TTSAA 7 cut(s) 353, 503, 580, 665, 940, 1475, 1547
AjnI CCWGG 5 cut(s) 223, 461, 567, 1088, 1538
AjuI GAANNNNNNNTTGG 4 cut(s) 488, 501, 520, 533
AleI CACNNNNGTG 2 cut(s) 81, 635
AloI GAACNNNNNNTCC 2 cut(s) 96, 128
AluBI AGCT 6 cut(s) 81, 128, 356, 868, 1288, 1531
AluI AGCT 6 cut(s) 81, 128, 356, 868, 1288, 1531
Alw21I GWGCWC 2 cut(s) 130, 400
Alw26I GTCTC 3 cut(s) 145, 223, 1433
AlwI GGATC 1 cut(s) 738
Ama87I CYCGRG 1 cut(s) 1234
Aor13HI TCCGGA 1 cut(s) 832
Aor51HI AGCGCT 1 cut(s) 1077
AoxI GGCC 1 cut(s) 422
ApaI GGGCCC 1 cut(s) 426
ApeKI GCWGC 4 cut(s) 810, 1020, 1188, 1398
ApoI RAATTY 2 cut(s) 581, 1451
AseI ATTAAT 3 cut(s) 912, 1122, 1332
AspLEI GCGC 1 cut(s) 1078
AspS9I GGNCC 3 cut(s) 422, 423, 1150
AsuC2I CCSGG 1 cut(s) 880
AsuII TTCGAA 1 cut(s) 507
AvaI CYCGRG 1 cut(s) 1234
AvaII GGWCC 1 cut(s) 1150
BaeGI GKGCMC 1 cut(s) 426
BanII GRGCYC 2 cut(s) 130, 426
BauI CACGAG 1 cut(s) 1132
BbsI GAAGAC 3 cut(s) 60, 1172, 1382
Bbv12I GWGCWC 2 cut(s) 130, 400
BbvI GCAGC 4 cut(s) 797, 1007, 1200, 1410
BccI CCATC 1 cut(s) 499
BceAI ACGGC 1 cut(s) 418
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 5 cut(s) 225, 463, 569, 1090, 1540
BciVI GTATCC 1 cut(s) 464
BcnI CCSGG 1 cut(s) 880
BcoDI GTCTC 3 cut(s) 145, 223, 1433
BfaI CTAG 4 cut(s) 20, 131, 176, 441
BfoI RGCGCY 1 cut(s) 1079
BfrI CTTAAG 2 cut(s) 752, 962
BfuI GTATCC 1 cut(s) 464
BglII AGATCT 2 cut(s) 782, 992
BisI GCNGC 4 cut(s) 811, 1021, 1189, 1399
BlsI GCNGC 4 cut(s) 812, 1022, 1190, 1400
Bme1390I CCNGG 6 cut(s) 225, 463, 569, 880, 1090, 1540
Bme18I GGWCC 1 cut(s) 1150
BmeT110I CYCGRG 1 cut(s) 1234
BmgT120I GGNCC 3 cut(s) 422, 423, 1150
BmiI GGNNCC 2 cut(s) 424, 565
BmrFI CCNGG 6 cut(s) 225, 463, 569, 880, 1090, 1540
BmrI ACTGGG 1 cut(s) 429
BmsI GCATC 2 cut(s) 153, 329
BmuI ACTGGG 1 cut(s) 429
BpiI GAAGAC 3 cut(s) 60, 1172, 1382
BpmI CTGGAG 2 cut(s) 179, 1009
Bpu10I CCTNAGC 1 cut(s) 1634
Bpu14I TTCGAA 1 cut(s) 507
BpuEI CTTGAG 8 cut(s) 371, 464, 632, 668, 842, 1052, 1193, 1403
BpuMI CCSGG 1 cut(s) 880
Bsa29I ATCGAT 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 224, 567, 1538
BsaWI WCCGGW 1 cut(s) 832
BsaXI ACNNNNNCTCC 2 cut(s) 220, 250
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 556
Bse1I ACTGG 3 cut(s) 162, 424, 1026
BseAI TCCGGA 1 cut(s) 832
BseBI CCWGG 5 cut(s) 225, 463, 569, 1090, 1540
BseCI ATCGAT 1 cut(s) 120
BseDI CCNNGG 3 cut(s) 224, 567, 1538
BseGI GGATG 3 cut(s) 344, 372, 491
BseLI CCNNNNNNNGG 2 cut(s) 101, 556
BseMII CTCAG 2 cut(s) 342, 725
BseNI ACTGG 3 cut(s) 162, 424, 1026
BseSI GKGCMC 1 cut(s) 426
BseXI GCAGC 4 cut(s) 797, 1007, 1200, 1410
BseYI CCCAGC 2 cut(s) 764, 974
BsgI GTGCAG 1 cut(s) 59
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 1 cut(s) 424
BshVI ATCGAT 1 cut(s) 120
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 2 cut(s) 130, 400
BsiHKCI CYCGRG 1 cut(s) 1234
BsiSI CCGG 3 cut(s) 814, 833, 879
BslI CCNNNNNNNGG 2 cut(s) 101, 556
BsmAI GTCTC 3 cut(s) 145, 223, 1433
BsmBI CGTCTC 2 cut(s) 223, 1433
BsmI GAATGC 2 cut(s) 1191, 1503
BsnI GGCC 1 cut(s) 424
BsoBI CYCGRG 1 cut(s) 1234
Bsp119I TTCGAA 1 cut(s) 507
Bsp120I GGGCCC 1 cut(s) 422
Bsp1286I GDGCHC 3 cut(s) 130, 400, 426
Bsp13I TCCGGA 1 cut(s) 832
Bsp1407I TGTACA 3 cut(s) 671, 1445, 1597
Bsp143I GATC 7 cut(s) 121, 390, 730, 782, 992, 1009, 1219
BspACI CCGC 1 cut(s) 744
BspANI GGCC 1 cut(s) 424
BspCNI CTCAG 2 cut(s) 341, 726
BspDI ATCGAT 1 cut(s) 120
BspEI TCCGGA 1 cut(s) 832
BspLI GGNNCC 2 cut(s) 424, 565
BspPI GGATC 1 cut(s) 738
BspQI GCTCTTC 1 cut(s) 1278
BspT104I TTCGAA 1 cut(s) 507
BspTI CTTAAG 2 cut(s) 752, 962
BsrGI TGTACA 3 cut(s) 671, 1445, 1597
BsrI ACTGG 3 cut(s) 162, 424, 1026
BssECI CCNNGG 3 cut(s) 224, 567, 1538
BssMI GATC 7 cut(s) 121, 390, 730, 782, 992, 1009, 1219
BssSI CACGAG 1 cut(s) 1132
Bst2BI CACGAG 1 cut(s) 1132
Bst2UI CCWGG 5 cut(s) 225, 463, 569, 1090, 1540
Bst4CI ACNGT 4 cut(s) 59, 139, 298, 1427
Bst6I CTCTTC 1 cut(s) 1278
BstAFI CTTAAG 2 cut(s) 752, 962
BstAUI TGTACA 3 cut(s) 671, 1445, 1597
BstBI TTCGAA 1 cut(s) 507
BstC8I GCNNGC 2 cut(s) 746, 1630
BstDEI CTNAG 5 cut(s) 328, 377, 679, 734, 1634
BstF5I GGATG 3 cut(s) 344, 372, 491
BstH2I RGCGCY 1 cut(s) 1079
BstHHI GCGC 1 cut(s) 1078
BstKTI GATC 7 cut(s) 124, 393, 733, 785, 995, 1012, 1222
BstMAI GTCTC 3 cut(s) 145, 223, 1433
BstMBI GATC 7 cut(s) 121, 390, 730, 782, 992, 1009, 1219
BstMCI CGRYCG 1 cut(s) 124
BstNI CCWGG 5 cut(s) 225, 463, 569, 1090, 1540
BstNSI RCATGY 2 cut(s) 33, 532
BstSCI CCNGG 6 cut(s) 223, 461, 567, 878, 1088, 1538
BstSLI GKGCMC 1 cut(s) 426
BstV1I GCAGC 4 cut(s) 797, 1007, 1200, 1410
BstV2I GAAGAC 3 cut(s) 60, 1172, 1382
BstX2I RGATCY 3 cut(s) 730, 782, 992
BstYI RGATCY 3 cut(s) 730, 782, 992
Bsu15I ATCGAT 1 cut(s) 120
BsuI GTATCC 1 cut(s) 464
BsuRI GGCC 1 cut(s) 424
BsuTUI ATCGAT 1 cut(s) 120
BtsCI GGATG 3 cut(s) 344, 372, 491
BtsIMutI CAGTG 1 cut(s) 400
Cac8I GCNNGC 2 cut(s) 746, 1630
CfoI GCGC 1 cut(s) 1078
Cfr13I GGNCC 3 cut(s) 422, 423, 1150
ClaI ATCGAT 1 cut(s) 120
Csp6I GTAC 4 cut(s) 288, 672, 1446, 1598
CviAII CATG 5 cut(s) 30, 167, 266, 529, 955
CviQI GTAC 4 cut(s) 288, 672, 1446, 1598
DdeI CTNAG 5 cut(s) 328, 377, 679, 734, 1634
DpnI GATC 7 cut(s) 123, 392, 732, 784, 994, 1011, 1221
DpnII GATC 7 cut(s) 121, 390, 730, 782, 992, 1009, 1219
DraI TTTAAA 1 cut(s) 40
Eam1104I CTCTTC 1 cut(s) 1278
EarI CTCTTC 1 cut(s) 1278
Ecl136II GAGCTC 1 cut(s) 128
Eco24I GRGCYC 2 cut(s) 130, 426
Eco32I GATATC 1 cut(s) 13
Eco47I GGWCC 1 cut(s) 1150
Eco47III AGCGCT 1 cut(s) 1077
Eco53kI GAGCTC 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 1547
Eco88I CYCGRG 1 cut(s) 1234
EcoICRI GAGCTC 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 423
EcoRII CCWGG 5 cut(s) 223, 461, 567, 1088, 1538
EcoRV GATATC 1 cut(s) 13
EcoT22I ATGCAT 2 cut(s) 168, 956
EcoT38I GRGCYC 2 cut(s) 130, 426
Esp3I CGTCTC 2 cut(s) 223, 1433
FaeI CATG 5 cut(s) 33, 170, 269, 532, 958
FalI AAGNNNNNCTT 1 cut(s) 31
FatI CATG 5 cut(s) 29, 166, 265, 528, 954
FauI CCCGC 1 cut(s) 737
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 4 cut(s) 811, 1021, 1189, 1399
FokI GGATG 3 cut(s) 351, 379, 478
FriOI GRGCYC 2 cut(s) 130, 426
Fsp4HI GCNGC 4 cut(s) 811, 1021, 1189, 1399
FspBI CTAG 4 cut(s) 20, 131, 176, 441
GlaI GCGC 1 cut(s) 1077
GluI GCNGC 4 cut(s) 811, 1021, 1189, 1399
GsaI CCCAGC 2 cut(s) 768, 978
GsuI CTGGAG 2 cut(s) 179, 1009
HaeII RGCGCY 1 cut(s) 1079
HaeIII GGCC 1 cut(s) 424
HapII CCGG 3 cut(s) 814, 833, 879
HhaI GCGC 1 cut(s) 1078
Hin1II CATG 5 cut(s) 33, 170, 269, 532, 958
Hin6I GCGC 1 cut(s) 1076
HinP1I GCGC 1 cut(s) 1076
HincII GTYRAC 1 cut(s) 1409
HindII GTYRAC 1 cut(s) 1409
HindIII AAGCTT 2 cut(s) 354, 866
HinfI GANTC 9 cut(s) 209, 317, 757, 817, 931, 967, 1177, 1237, 1387
HpaII CCGG 3 cut(s) 814, 833, 879
Hpy166II GTNNAC 5 cut(s) 202, 674, 691, 1409, 1600
Hpy188I TCNGA 7 cut(s) 192, 331, 735, 844, 1054, 1264, 1578
Hpy8I GTNNAC 5 cut(s) 202, 674, 691, 1409, 1600
HpyAV CCTTC 3 cut(s) 142, 554, 1481
HpyCH4III ACNGT 4 cut(s) 59, 139, 298, 1427
HpyCH4V TGCA 9 cut(s) 29, 62, 76, 166, 532, 954, 1164, 1374, 1587
HpyF3I CTNAG 5 cut(s) 328, 377, 679, 734, 1634
Hsp92II CATG 5 cut(s) 33, 170, 269, 532, 958
HspAI GCGC 1 cut(s) 1076
Kpn2I TCCGGA 1 cut(s) 832
Kzo9I GATC 7 cut(s) 121, 390, 730, 782, 992, 1009, 1219
LguI GCTCTTC 1 cut(s) 1278
LmnI GCTCC 1 cut(s) 1028
Lsp1109I GCAGC 4 cut(s) 797, 1007, 1200, 1410
LweI GCATC 2 cut(s) 153, 329
MaeI CTAG 4 cut(s) 20, 131, 176, 441
MaeIII GTNAC 2 cut(s) 32, 133
MalI GATC 7 cut(s) 123, 392, 732, 784, 994, 1011, 1221
MboI GATC 7 cut(s) 121, 390, 730, 782, 992, 1009, 1219
MboII GAAGA 6 cut(s) 65, 266, 1172, 1295, 1382, 1487
MfeI CAATTG 2 cut(s) 1159, 1441
MflI RGATCY 3 cut(s) 730, 782, 992
MhlI GDGCHC 3 cut(s) 130, 400, 426
MlyI GAGTC 5 cut(s) 826, 940, 1186, 1246, 1396
MmeI TCCRAC 2 cut(s) 499, 519
Mph1103I ATGCAT 2 cut(s) 168, 956
MroI TCCGGA 1 cut(s) 832
MslI CAYNNNNRTG 3 cut(s) 81, 635, 1196
MspA1I CMGCKG 1 cut(s) 81
MspCI CTTAAG 2 cut(s) 752, 962
MspI CCGG 3 cut(s) 814, 833, 879
MspR9I CCNGG 6 cut(s) 225, 463, 569, 880, 1090, 1540
MunI CAATTG 2 cut(s) 1159, 1441
Mva1269I GAATGC 2 cut(s) 1191, 1503
MvaI CCWGG 5 cut(s) 225, 463, 569, 1090, 1540
NciI CCSGG 1 cut(s) 880
NdeII GATC 7 cut(s) 121, 390, 730, 782, 992, 1009, 1219
NlaIII CATG 5 cut(s) 33, 170, 269, 532, 958
NlaIV GGNNCC 2 cut(s) 424, 565
NsiI ATGCAT 2 cut(s) 168, 956
NspI RCATGY 2 cut(s) 33, 532
NspV TTCGAA 1 cut(s) 507
OliI CACNNNNGTG 2 cut(s) 81, 635
PaeR7I CTCGAG 1 cut(s) 1234
PciSI GCTCTTC 1 cut(s) 1278
PctI GAATGC 2 cut(s) 1191, 1503
PfeI GAWTC 4 cut(s) 209, 317, 757, 967
PkrI GCNGC 4 cut(s) 812, 1022, 1190, 1400
Ple19I CGATCG 1 cut(s) 124
PleI GAGTC 5 cut(s) 825, 939, 1185, 1245, 1395
PpsI GAGTC 5 cut(s) 825, 939, 1185, 1245, 1395
PshBI ATTAAT 3 cut(s) 912, 1122, 1332
Psp124BI GAGCTC 1 cut(s) 130
Psp6I CCWGG 5 cut(s) 223, 461, 567, 1088, 1538
PspFI CCCAGC 2 cut(s) 764, 974
PspGI CCWGG 5 cut(s) 223, 461, 567, 1088, 1538
PspN4I GGNNCC 2 cut(s) 424, 565
PspOMI GGGCCC 1 cut(s) 422
PspPI GGNCC 3 cut(s) 422, 423, 1150
PspXI VCTCGAGB 1 cut(s) 1234
PsuI RGATCY 3 cut(s) 730, 782, 992
PvuI CGATCG 1 cut(s) 124
PvuII CAGCTG 1 cut(s) 81
RsaI GTAC 4 cut(s) 289, 673, 1447, 1599
RsaNI GTAC 4 cut(s) 288, 672, 1446, 1598
RseI CAYNNNNRTG 3 cut(s) 81, 635, 1196
SacI GAGCTC 1 cut(s) 130
SapI GCTCTTC 1 cut(s) 1278
SatI GCNGC 4 cut(s) 811, 1021, 1189, 1399
Sau3AI GATC 7 cut(s) 121, 390, 730, 782, 992, 1009, 1219
Sau96I GGNCC 3 cut(s) 422, 423, 1150
SchI GAGTC 5 cut(s) 826, 940, 1186, 1246, 1396
ScrFI CCNGG 6 cut(s) 225, 463, 569, 880, 1090, 1540
SduI GDGCHC 3 cut(s) 130, 400, 426
SfaNI GCATC 2 cut(s) 153, 329
Sfr274I CTCGAG 1 cut(s) 1234
SfuI TTCGAA 1 cut(s) 507
SinI GGWCC 1 cut(s) 1150
SlaI CTCGAG 1 cut(s) 1234
SmiMI CAYNNNNRTG 3 cut(s) 81, 635, 1196
SsiI CCGC 1 cut(s) 744
SspMI CTAG 4 cut(s) 20, 131, 176, 441
SstI GAGCTC 1 cut(s) 130
StyD4I CCNGG 6 cut(s) 223, 461, 567, 878, 1088, 1538
TaaI ACNGT 4 cut(s) 59, 139, 298, 1427
TaqI TCGA 7 cut(s) 120, 124, 300, 315, 507, 698, 1235
TaqII GACCGA 1 cut(s) 564
TatI WGTACW 3 cut(s) 671, 1445, 1597
TfiI GAWTC 4 cut(s) 209, 317, 757, 967
TscAI CASTG 1 cut(s) 400
TseI GCWGC 4 cut(s) 810, 1020, 1188, 1398
TspDTI ATGAA 5 cut(s) 254, 267, 574, 1214, 1382
TspRI CASTG 1 cut(s) 400
Vha464I CTTAAG 2 cut(s) 752, 962
VpaK11BI GGWCC 1 cut(s) 1150
VspI ATTAAT 3 cut(s) 912, 1122, 1332
XapI RAATTY 2 cut(s) 581, 1451
XbaI TCTAGA 1 cut(s) 19
XceI RCATGY 2 cut(s) 33, 532
XhoI CTCGAG 1 cut(s) 1234
XmiI GTMKAC 1 cut(s) 201
XspI CTAG 4 cut(s) 20, 131, 176, 441
Zsp2I ATGCAT 2 cut(s) 168, 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.