MD07G1242100.v1.1

RESISTANCE protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000067
Physical Location & Seq
Reverse (-)
31257802 .. 31262494
4693 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1242100.v1.1.491

Sequence Viewer

Length: 3759 bp
ATGGCTGCTTCTTCTTCTTCATCTTCTTCTGGCCTTGGTTGCAAATACGATGTGTTCATCAATTTCAGAGGGGAAGACACTCGCAGGGGCTTCGTCAGCCATCTCTACAACGCTCTGGTTAAGAAACCAATCAACGCCTTCATTGATGCCGAGAAGCTCAGAAAAGGCGACCACCTTTCTGAGCTCCTGACAGCGATTCGAGAGTCAAGGCTTTCGATTGTAGTTTTCTCTCAAGACTATGCTTCTTCCACTTGGTGCTTGAAAGAACTCGTGCAAATCTTGAAATGCAAGGATACCAATAACCAGATTGTACTCCCCATTTTCTATGAAGTTGATCCGTCTGACGTTCGTAAACTCAAGAGAAAATTCGAGGAAGCTTTTGCTCAGCACGATCGTGATTCTAACGCTGAAATGGAAGAGGTTCAGAGCTGGAGATCCGCTCTTACAACTGCCACCAGTTTATCCGGCTGGGATTCGCGAAAATATGAGAATGATGCAGAGCTTATTGAGGAAATTGTAGAATATGTTTATAGGAAATTGATCGACATCTCATCAACATCAAGCAAAGATAATGGCTTGGTTGACATGGATTCTCACATGCATGAAATGCATTTATTATTATATCCTCCCGGAGGTGAAGCGAATAATGTTCGCGTTGTTGGAATATGGGGTATGGGTGGTTTAGGCAAAACAACCATCGCTAGAGCAGTTTATGATGAAATCGCATGTCGATTTGAAGCTTCTTGCTTTCTTGAAAACGTCAAGGAGGGTTTCATGAAGCATGGCAAACCACATATGCAGACACAACTTCTATCGAGTATATCGAACAACAAGGTGGGGAGTTCTGACATATCAAAATATGGTTTTAAGAAGATGTTAAAAAGACTTGGTCGGAGAAAAGTTCTTATTGTTGTTGATGATGTGGACGAATCAGAACAAATTGAAGCTTTACTTGGAAAGAAACGTTCCTTTGGTGTTGGAAGTAGGATTATTATAACAAGTAGAGATTCGCAGTTACTAAGCATAACTGATGTGATATATAATCCCCGGACTTTGAGTTATTATGGAGCTCTGGAACTCTTTTGGCAGTACGCCTTCAAAAATGACCAACCCAACAGAGATTATTATGATCTCTCGAATCGTGTTGTAGAATATGCTCAAGGTCTGCCTTTAGCCCTCAAAGTCTTGGGAGCTTTTTTGCATAAAAAAACTATACGCGTGTGGGAAGGTGAGTTAGAAAAAATAAGGAAAATCCCACAAAGGGGAGTTCATGATGTGCTTAAAACAAGCTTCGATGGACTAGATGACACACAGAAGGACATCTTTCTAGATATTGCATGTTTCTTTAGAGGGATGAAGGAAGACCAGGCAACTGAAATTCTGGACAGTTGTGGTTTCCATCCTCATACAGGAATAAAAGTTCTAATTGATCGAGCTCTCATAACTGTCTCAACGTTGGGGGTATTGGAGATGCATGATTCATTAGAGGAAATGGGTCGGGAAATTGTCCGCCAAGAATCTAGCAAAGATCCTGGGAGACGAAGTAGGTTGTGGAGTTATGAAGATGTTTGTGAGGTGCTAACTGAAAATACGGCTACAAAAACAGTTGAAAGCATAATTGTGGATTTCTCGTACTCAAGCTGGCCATGCTTAAATGCTGAAGCTTTTGTTAGAATGACTCAACTAAGACTTCTCAAGATCAATCATAGGCACATAAATGCTGAAGCTGTTGTTAGAAGGACTCAACGAAGACTTCTCAAGATCATTTATAGGCACTTCGTTTTTAAAGATGAGTGCTGCAAACACCACCTGTTTGGGTCCTTAAAGTTGCTTAACTTGAGATATCTCTCCTGGCTTGGTTTCCCTCTCAAGTCTTTGCCATCCAACTTTCAATTCGAAAATCTTGTTGAACTTGACATGCAATATAGTCTCATTGACCGACTTTGGGAAGGAACCCAGACACAGGAAAAGTTGAAATTCATCAATTTAAGTAATTGTCAATCCCTTAAGGAAACCCCTGACTTCACAAATGTGCCAAATCTTGAGAGGCTAATTGTTCGAAGTTGTATAAGCTTAGTTGAGGTTCACCCATCTATTTCGACTCTTTCAAACCTTGTTTTATTGGATCTGCATGGGTGCAATGAACTTATGATTCTACCTACCAAAATTCGTATGAGATCTCTCAAGACCTTTAATCTTTCTGGTTGCTCGAACCTTGAGATGTTTCCAGAGATTTCAGAAGTGATTGAGGGGTTTGAAGAGCTTGATTTATCCGGATCAAGAATTAAAGAACTGCCCCCGTCAATTAATAATCTCACGGGGTTGAGTCATTTGAACCTATCAAATTGCAGGGAACTTAAGATTCTACCTAGCAAAATTCCTATGAGATCTCTCAAAACCTTTAATCTTTCTTACTGCTCCAGTCTTGAGATGTTTCCAGAGATTTCAGAAGGTATGGAGGAGTTAAAAGAGCTTAATTTATCCGGGTTAAAAATTAAAGAACTGCCCCTGTCAATTAATAATCTCTCAGGGTTGAGTCATTTCAACCTAAAAGATTGCAAGGAACTTAAGATTCTACCTAGCAAAATTCGTATGAGATCTCTCAAAACCTTTGATCTTTCTGACTGCTCCAGTCTTGAGATGTTTCCAGAGATTTCAGAAGGTATGGAGGAGTTAGAAGAGCTTAATTTATCCGGGTCAAAAATTAAAGAACTGCCCCTGTTAATTAATAATCTCTCGGGGTTGAGTCATTTCAACCTAAAAGATTGCAAGGAACTTAAGATTCTACCTAGCAAAATTCGTATGAGATCTCTCAAAACCTTTGATCTTTCTGATTGCTCCAGTCTTGAGATGTTTCCAGAGATTTCAGAAGGTATGGAGGAGTTAGAAGAGCTTAATTTATCCGGGTCAAAAATTAAAGAATTGCCCGAGTCAATTAATAATCTCACGGGGTTGAGTCATTTCAACCTAAAAAATTGCAAGGAACTTAAGAGTCTTCCAAGCTGCATTCATATGAAATGTCTGAAAACCTTTAATCTTGATGGCTGCTCCAGTCTAGAGATGTTTCCATCGATTTCAGAAGGTATTGAGGGGTTAGAAAAGCTTGATTTACCCTGCTCAAAAATTAAAGAACTGTCCCCGTCAATTAATAATCTTACGAGGTTGTGTCATTTGAATCTACAAGGTTGCAAGGAACTTAAGAGTCTTCCAAGCAGCATTCGTATGAAATCTCTCAAAACCTTTGATCTTAATGGTTGCTGGAGTCTTGAGATGTTTCCAGAGATTTCAGAAGTGATTGAGGGGTTAAAAGTTTTTGAGTTATCCGGGTCGAAAATTAAAGAACTGCCCTCGTCAATTAATAATCTTACGGGATTGAAACATTTGAAGCTAGAAAATTGTAAGGAACTTGAGAGTCTTCCGAGCAGCATTTGTCAGCTCAAGTCCCTTCTCGTTCTCTCTCTTTCCGGTTGTACAAAATTTGAGGTGTTTCCAAGCATTGAAGAAAATATGGAAGGATTAAGATGTCTTTTCTTGGATGGAACATCTATCAAAGAGCTTTCCCCCTGGATTGAACGGCTTACGGGGCTTCGGTATTTAGATCTGAGAAACGGCAAAAGCCTTGTACATCTTCCCAACACTCTCTGTAATTTGGCATGGGTTTTCACAGACACAGAGATCCGTCCTGGCCGGGGTCCCACTCCACCCCCACGTCGATTTCTAGCTCTGTTTTGGCTTGGGTGCTCGTTGCAGCAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_3 LRR_5 LRR_8 NB-ARC TIR

Protein Analysis

1253

Amino Acids

142.28

Weight (kDa)

6.39

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 15 - 194 2.4e-53 TIR domain
TIR_2 PF13676 18 - 119 4e-15 TIR domain
NB-ARC PF00931 214 - 369 2.6e-15 NB-ARC domain
WHD_ROQ1 PF23282 437 - 507 2.5e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 634 - 653 5.9e-06 Leucine Rich Repeat
LRR_14 PF23598 693 - 825 6.6e-06 Leucine-rich repeat region
LRR_13 PF23286 726 - 795 1.5e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_EndoS PF23952 753 - 850 2e-07 Endo-beta-N-acetylglucosaminidase EndoS LRR domain
LRR_13 PF23286 796 - 865 2.6e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 866 - 935 8.2e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 936 - 1019 5.7e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_EndoS PF23952 964 - 1037 9.3e-06 Endo-beta-N-acetylglucosaminidase EndoS LRR domain
LRR_14 PF23598 1067 - 1174 1.2e-10 Leucine-rich repeat region
LRR_13 PF23286 1076 - 1157 1.4e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 995
AccBSI CCGCTC 1 cut(s) 440
AccII CGCG 3 cut(s) 478, 654, 1218
AccIII TCCGGA 1 cut(s) 2273
AciI CCGC 2 cut(s) 438, 1510
AclI AACGTT 2 cut(s) 964, 1454
AclWI GGATC 6 cut(s) 329, 429, 1523, 2133, 2284, 3670
AcoI YGGCCR 2 cut(s) 1643, 3685
AcsI RAATTY 8 cut(s) 365, 1377, 1976, 2166, 2376, 2586, 2796, 3476
AcuI CTGAAG 2 cut(s) 1680, 1743
AdeI CACNNNGTG 1 cut(s) 255
AfaI GTAC 5 cut(s) 312, 1091, 1634, 3472, 3624
AfiI CCNNNNNNNGG 7 cut(s) 632, 1261, 1262, 1409, 1945, 1963, 3689
AflII CTTAAG 6 cut(s) 2006, 2357, 2567, 2777, 2987, 3197
AflIII ACRYGT 1 cut(s) 1216
AjiI CACGTC 1 cut(s) 3710
AjnI CCWGG 5 cut(s) 1365, 1531, 1850, 3563, 3682
AjuI GAANNNNNNNTTGG 4 cut(s) 936, 968, 1877, 1909
AleI CACNNNNGTG 2 cut(s) 393, 2030
AloI GAACNNNNNNTCC 2 cut(s) 1404, 1436
Alw21I GWGCWC 4 cut(s) 186, 1072, 1438, 3743
Alw26I GTCTC 3 cut(s) 1453, 1531, 1934
AlwI GGATC 6 cut(s) 329, 429, 1523, 2133, 2284, 3670
Ama87I CYCGRG 2 cut(s) 2737, 2927
Aor13HI TCCGGA 1 cut(s) 2273
AoxI GGCC 3 cut(s) 31, 1643, 3685
ApeKI GCWGC 8 cut(s) 5, 1797, 3003, 3045, 3213, 3423, 3748, 3751
ApoI RAATTY 8 cut(s) 365, 1377, 1976, 2166, 2376, 2586, 2796, 3476
AseI ATTAAT 6 cut(s) 2307, 2517, 2727, 2937, 3147, 3357
Asp700I GAANNNNTTC 1 cut(s) 420
AspS9I GGNCC 2 cut(s) 1818, 3692
AsuC2I CCSGG 7 cut(s) 630, 1048, 2485, 2695, 2905, 3325, 3689
AsuHPI GGTGA 3 cut(s) 647, 1241, 2078
AsuII TTCGAA 2 cut(s) 1896, 2059
AvaI CYCGRG 2 cut(s) 2737, 2927
AvaII GGWCC 2 cut(s) 1818, 3692
BalI TGGCCA 1 cut(s) 1645
BanII GRGCYC 3 cut(s) 186, 1072, 1438
BauI CACGAG 1 cut(s) 269
BbsI GAAGAC 6 cut(s) 81, 1368, 1756, 2987, 3197, 3407
Bbv12I GWGCWC 4 cut(s) 186, 1072, 1438, 3743
BbvI GCAGC 5 cut(s) 1784, 2990, 3032, 3225, 3435
BccI CCATC 9 cut(s) 108, 704, 1289, 1407, 1888, 2098, 3035, 3076, 3530
BceAI ACGGC 3 cut(s) 1608, 3590, 3625
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciT130I CCWGG 5 cut(s) 1367, 1533, 1852, 3565, 3684
BciVI GTATCC 1 cut(s) 286
BcnI CCSGG 7 cut(s) 630, 1048, 2485, 2695, 2905, 3325, 3689
BcoDI GTCTC 3 cut(s) 1453, 1531, 1934
BfrI CTTAAG 6 cut(s) 2006, 2357, 2567, 2777, 2987, 3197
BfuI GTATCC 1 cut(s) 286
BglII AGATCT 5 cut(s) 2177, 2387, 2597, 2807, 3598
BisI GCNGC 8 cut(s) 6, 1798, 3004, 3046, 3214, 3424, 3749, 3752
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 8 cut(s) 7, 1799, 3005, 3047, 3215, 3425, 3750, 3753
Bme18I GGWCC 2 cut(s) 1818, 3692
BmeT110I CYCGRG 2 cut(s) 2737, 2927
BmgBI CACGTC 1 cut(s) 3710
BmgT120I GGNCC 2 cut(s) 1818, 3692
BmiI GGNNCC 4 cut(s) 1819, 1954, 3693, 3694
BmsI GCATC 3 cut(s) 136, 484, 1461
BpiI GAAGAC 6 cut(s) 81, 1368, 1756, 2987, 3197, 3407
BplI GAGNNNNNCTC 2 cut(s) 424, 456
BpmI CTGGAG 6 cut(s) 451, 2404, 2614, 2824, 3034, 3280
Bpu1102I GCTNAGC 1 cut(s) 384
Bpu14I TTCGAA 2 cut(s) 1896, 2059
BpuMI CCSGG 7 cut(s) 630, 1048, 2485, 2695, 2905, 3325, 3689
Bsa29I ATCGAT 1 cut(s) 3071
BsaBI GATNNNNATC 1 cut(s) 545
BsaJI CCNNGG 5 cut(s) 34, 1046, 1532, 3563, 3688
BsaWI WCCGGW 2 cut(s) 2273, 3464
BsaXI ACNNNNNCTCC 2 cut(s) 1528, 1558
Bsc4I CCNNNNNNNGG 7 cut(s) 632, 1261, 1262, 1409, 1945, 1963, 3689
Bse1I ACTGG 5 cut(s) 456, 2421, 2631, 2841, 3051
Bse3DI GCAATG 1 cut(s) 2146
Bse8I GATNNNNATC 1 cut(s) 545
BseAI TCCGGA 1 cut(s) 2273
BseBI CCWGG 5 cut(s) 1367, 1533, 1852, 3565, 3684
BseCI ATCGAT 1 cut(s) 3071
BseDI CCNNGG 5 cut(s) 34, 1046, 1532, 3563, 3688
BseGI GGATG 4 cut(s) 1359, 1399, 1880, 3541
BseJI GATNNNNATC 1 cut(s) 545
BseLI CCNNNNNNNGG 7 cut(s) 632, 1261, 1262, 1409, 1945, 1963, 3689
BseMI GCAATG 1 cut(s) 2146
BseMII CTCAG 5 cut(s) 171, 172, 398, 2541, 3593
BseNI ACTGG 5 cut(s) 456, 2421, 2631, 2841, 3051
BseRI GAGGAG 3 cut(s) 2474, 2684, 2894
BseXI GCAGC 5 cut(s) 1784, 2990, 3032, 3225, 3435
BseYI CCCAGC 1 cut(s) 468
Bsh1236I CGCG 3 cut(s) 478, 654, 1218
Bsh1285I CGRYCG 1 cut(s) 394
BshFI GGCC 3 cut(s) 33, 1645, 3687
BshVI ATCGAT 1 cut(s) 3071
BsiEI CGRYCG 1 cut(s) 394
BsiHKAI GWGCWC 4 cut(s) 186, 1072, 1438, 3743
BsiHKCI CYCGRG 2 cut(s) 2737, 2927
BslFI GGGAC 3 cut(s) 3121, 3427, 3678
BslI CCNNNNNNNGG 7 cut(s) 632, 1261, 1262, 1409, 1945, 1963, 3689
BsmAI GTCTC 3 cut(s) 1453, 1531, 1934
BsmBI CGTCTC 1 cut(s) 1531
BsmFI GGGAC 3 cut(s) 3121, 3427, 3678
BsmI GAATGC 2 cut(s) 3006, 3216
BsnI GGCC 3 cut(s) 33, 1645, 3687
BsoBI CYCGRG 2 cut(s) 2737, 2927
Bsp119I TTCGAA 2 cut(s) 1896, 2059
Bsp1286I GDGCHC 4 cut(s) 186, 1072, 1438, 3743
Bsp13I TCCGGA 1 cut(s) 2273
Bsp1407I TGTACA 2 cut(s) 3470, 3622
Bsp1720I GCTNAGC 1 cut(s) 384
Bsp68I TCGCGA 1 cut(s) 478
BspACI CCGC 2 cut(s) 438, 1510
BspANI GGCC 3 cut(s) 33, 1645, 3687
BspCNI CTCAG 5 cut(s) 171, 172, 397, 2540, 3594
BspDI ATCGAT 1 cut(s) 3071
BspEI TCCGGA 1 cut(s) 2273
BspFNI CGCG 3 cut(s) 478, 654, 1218
BspHI TCATGA 2 cut(s) 774, 1270
BspLI GGNNCC 4 cut(s) 1819, 1954, 3693, 3694
BspPI GGATC 6 cut(s) 329, 429, 1523, 2133, 2284, 3670
BspQI GCTCTTC 3 cut(s) 2253, 2673, 2883
BspT104I TTCGAA 2 cut(s) 1896, 2059
BspTI CTTAAG 6 cut(s) 2006, 2357, 2567, 2777, 2987, 3197
BsrBI CCGCTC 1 cut(s) 440
BsrDI GCAATG 1 cut(s) 2146
BsrGI TGTACA 2 cut(s) 3470, 3622
BsrI ACTGG 5 cut(s) 456, 2421, 2631, 2841, 3051
BssECI CCNNGG 5 cut(s) 34, 1046, 1532, 3563, 3688
BssSI CACGAG 1 cut(s) 269
BssT1I CCWWGG 1 cut(s) 34
Bst2BI CACGAG 1 cut(s) 269
Bst2UI CCWGG 5 cut(s) 1367, 1533, 1852, 3565, 3684
Bst4CI ACNGT 4 cut(s) 1388, 1447, 1606, 3135
Bst6I CTCTTC 4 cut(s) 411, 2253, 2673, 2883
BstAFI CTTAAG 6 cut(s) 2006, 2357, 2567, 2777, 2987, 3197
BstAPI GCANNNNNTGC 1 cut(s) 607
BstAUI TGTACA 2 cut(s) 3470, 3622
BstBI TTCGAA 2 cut(s) 1896, 2059
BstC8I GCNNGC 1 cut(s) 1643
BstDEI CTNAG 8 cut(s) 158, 180, 384, 1019, 1685, 2074, 2527, 3602
BstENI CCTNNNNNAGG 2 cut(s) 630, 1407
BstF5I GGATG 4 cut(s) 1359, 1399, 1880, 3541
BstFNI CGCG 3 cut(s) 478, 654, 1218
BstMAI GTCTC 3 cut(s) 1453, 1531, 1934
BstMCI CGRYCG 1 cut(s) 394
BstMWI GCNNNNNNNGC 5 cut(s) 39, 96, 607, 1647, 3583
BstNI CCWGG 5 cut(s) 1367, 1533, 1852, 3565, 3684
BstNSI RCATGY 4 cut(s) 601, 729, 1341, 1921
BstUI CGCG 3 cut(s) 478, 654, 1218
BstV1I GCAGC 5 cut(s) 1784, 2990, 3032, 3225, 3435
BstV2I GAAGAC 6 cut(s) 81, 1368, 1756, 2987, 3197, 3407
BstX2I RGATCY 9 cut(s) 434, 1528, 2125, 2177, 2387, 2597, 2807, 3598, 3675
BstXI CCANNNNNNTGG 1 cut(s) 1814
BstYI RGATCY 9 cut(s) 434, 1528, 2125, 2177, 2387, 2597, 2807, 3598, 3675
Bsu15I ATCGAT 1 cut(s) 3071
BsuI GTATCC 1 cut(s) 286
BsuRI GGCC 3 cut(s) 33, 1645, 3687
BsuTUI ATCGAT 1 cut(s) 3071
BtgZI GCGATG 1 cut(s) 682
BtrI CACGTC 1 cut(s) 3710
BtsCI GGATG 4 cut(s) 1359, 1399, 1880, 3541
BtuMI TCGCGA 1 cut(s) 478
Cac8I GCNNGC 1 cut(s) 1643
CciI TCATGA 2 cut(s) 774, 1270
Cfr13I GGNCC 2 cut(s) 1818, 3692
ClaI ATCGAT 1 cut(s) 3071
Csp6I GTAC 5 cut(s) 311, 1090, 1633, 3471, 3623
CviQI GTAC 5 cut(s) 311, 1090, 1633, 3471, 3623
DdeI CTNAG 8 cut(s) 158, 180, 384, 1019, 1685, 2074, 2527, 3602
DraI TTTAAA 1 cut(s) 1786
DraIII CACNNNGTG 1 cut(s) 255
EaeI YGGCCR 2 cut(s) 1643, 3685
Eam1104I CTCTTC 4 cut(s) 411, 2253, 2673, 2883
EarI CTCTTC 4 cut(s) 411, 2253, 2673, 2883
EciI GGCGGA 1 cut(s) 1499
Ecl136II GAGCTC 3 cut(s) 184, 1070, 1436
Eco130I CCWWGG 1 cut(s) 34
Eco24I GRGCYC 3 cut(s) 186, 1072, 1438
Eco32I GATATC 1 cut(s) 1844
Eco47I GGWCC 2 cut(s) 1818, 3692
Eco53kI GAGCTC 3 cut(s) 184, 1070, 1436
Eco57I CTGAAG 2 cut(s) 1680, 1743
Eco88I CYCGRG 2 cut(s) 2737, 2927
EcoICRI GAGCTC 3 cut(s) 184, 1070, 1436
EcoNI CCTNNNNNAGG 2 cut(s) 630, 1407
EcoO109I RGGNCCY 2 cut(s) 1818, 3692
EcoRII CCWGG 5 cut(s) 1365, 1531, 1850, 3563, 3682
EcoRV GATATC 1 cut(s) 1844
EcoT14I CCWWGG 1 cut(s) 34
EcoT22I ATGCAT 3 cut(s) 603, 612, 1476
EcoT38I GRGCYC 3 cut(s) 186, 1072, 1438
ErhI CCWWGG 1 cut(s) 34
Esp3I CGTCTC 1 cut(s) 1531
FalI AAGNNNNNCTT 4 cut(s) 936, 968, 1307, 1339
FaqI GGGAC 3 cut(s) 3121, 3427, 3678
FauNDI CATATG 2 cut(s) 795, 3012
Fnu4HI GCNGC 8 cut(s) 6, 1798, 3004, 3046, 3214, 3424, 3749, 3752
FokI GGATG 4 cut(s) 1366, 1386, 1867, 3548
FriOI GRGCYC 3 cut(s) 186, 1072, 1438
Fsp4HI GCNGC 8 cut(s) 6, 1798, 3004, 3046, 3214, 3424, 3749, 3752
GluI GCNGC 8 cut(s) 6, 1798, 3004, 3046, 3214, 3424, 3749, 3752
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 6 cut(s) 451, 2404, 2614, 2824, 3034, 3280
HaeIII GGCC 3 cut(s) 33, 1645, 3687
HincII GTYRAC 1 cut(s) 583
HindII GTYRAC 1 cut(s) 583
HindIII AAGCTT 7 cut(s) 375, 738, 945, 1288, 1662, 2071, 3101
HphI GGTGA 3 cut(s) 647, 1241, 2078
Hpy166II GTNNAC 4 cut(s) 353, 583, 925, 2086
Hpy8I GTNNAC 4 cut(s) 353, 583, 925, 2086
Hpy99I CGWCG 1 cut(s) 3714
HpyCH4III ACNGT 4 cut(s) 1388, 1447, 1606, 3135
HpyCH4IV ACGT 5 cut(s) 345, 759, 964, 1454, 3709
HpyF10VI GCNNNNNNNGC 5 cut(s) 39, 96, 607, 1647, 3583
HpyF3I CTNAG 8 cut(s) 158, 180, 384, 1019, 1685, 2074, 2527, 3602
HpySE526I ACGT 5 cut(s) 345, 759, 964, 1454, 3709
KflI GGGWCCC 1 cut(s) 3692
Kpn2I TCCGGA 1 cut(s) 2273
LguI GCTCTTC 3 cut(s) 2253, 2673, 2883
LmnI GCTCC 7 cut(s) 189, 1067, 1190, 2423, 2633, 2843, 3053
Lsp1109I GCAGC 5 cut(s) 1784, 2990, 3032, 3225, 3435
LweI GCATC 3 cut(s) 136, 484, 1461
MaeII ACGT 5 cut(s) 345, 759, 964, 1454, 3709
MaeIII GTNAC 1 cut(s) 1014
MbiI CCGCTC 1 cut(s) 440
MflI RGATCY 9 cut(s) 434, 1528, 2125, 2177, 2387, 2597, 2807, 3598, 3675
MhlI GDGCHC 4 cut(s) 186, 1072, 1438, 3743
MlsI TGGCCA 1 cut(s) 1645
MluI ACGCGT 1 cut(s) 1216
MluNI TGGCCA 1 cut(s) 1645
MmeI TCCRAC 4 cut(s) 640, 872, 958, 1908
Mox20I TGGCCA 1 cut(s) 1645
Mph1103I ATGCAT 3 cut(s) 603, 612, 1476
MroI TCCGGA 1 cut(s) 2273
MroXI GAANNNNTTC 1 cut(s) 420
MscI TGGCCA 1 cut(s) 1645
MslI CAYNNNNRTG 7 cut(s) 393, 600, 1619, 1716, 2030, 3011, 3221
Msp20I TGGCCA 1 cut(s) 1645
MspCI CTTAAG 6 cut(s) 2006, 2357, 2567, 2777, 2987, 3197
Mva1269I GAATGC 2 cut(s) 3006, 3216
MvaI CCWGG 5 cut(s) 1367, 1533, 1852, 3565, 3684
MvnI CGCG 3 cut(s) 478, 654, 1218
MwoI GCNNNNNNNGC 5 cut(s) 39, 96, 607, 1647, 3583
NciI CCSGG 7 cut(s) 630, 1048, 2485, 2695, 2905, 3325, 3689
NdeI CATATG 2 cut(s) 795, 3012
NlaIV GGNNCC 4 cut(s) 1819, 1954, 3693, 3694
NmeAIII GCCGAG 1 cut(s) 175
NruI TCGCGA 1 cut(s) 478
NsiI ATGCAT 3 cut(s) 603, 612, 1476
NspI RCATGY 4 cut(s) 601, 729, 1341, 1921
NspV TTCGAA 2 cut(s) 1896, 2059
OliI CACNNNNGTG 2 cut(s) 393, 2030
PacI TTAATTAA 1 cut(s) 2727
PagI TCATGA 2 cut(s) 774, 1270
PciSI GCTCTTC 3 cut(s) 2253, 2673, 2883
PcsI WCGNNNNNNNCGW 1 cut(s) 821
PctI GAATGC 2 cut(s) 3006, 3216
PdmI GAANNNNTTC 1 cut(s) 420
PflFI GACNNNGTC 1 cut(s) 888
PfoI TCCNGGA 1 cut(s) 628
PkrI GCNGC 8 cut(s) 7, 1799, 3005, 3047, 3215, 3425, 3750, 3753
Ple19I CGATCG 1 cut(s) 394
PpuMI RGGWCCY 2 cut(s) 1818, 3692
PshBI ATTAAT 6 cut(s) 2307, 2517, 2727, 2937, 3147, 3357
PsiI TTATAA 1 cut(s) 995
Psp124BI GAGCTC 3 cut(s) 186, 1072, 1438
Psp1406I AACGTT 2 cut(s) 964, 1454
Psp5II RGGWCCY 2 cut(s) 1818, 3692
Psp6I CCWGG 5 cut(s) 1365, 1531, 1850, 3563, 3682
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 5 cut(s) 1365, 1531, 1850, 3563, 3682
PspN4I GGNNCC 4 cut(s) 1819, 1954, 3693, 3694
PspPI GGNCC 2 cut(s) 1818, 3692
PspPPI RGGWCCY 2 cut(s) 1818, 3692
PsuI RGATCY 9 cut(s) 434, 1528, 2125, 2177, 2387, 2597, 2807, 3598, 3675
PsyI GACNNNGTC 1 cut(s) 888
PvuI CGATCG 1 cut(s) 394
RruI TCGCGA 1 cut(s) 478
RsaI GTAC 5 cut(s) 312, 1091, 1634, 3472, 3624
RsaNI GTAC 5 cut(s) 311, 1090, 1633, 3471, 3623
RseI CAYNNNNRTG 7 cut(s) 393, 600, 1619, 1716, 2030, 3011, 3221
SacI GAGCTC 3 cut(s) 186, 1072, 1438
SapI GCTCTTC 3 cut(s) 2253, 2673, 2883
SatI GCNGC 8 cut(s) 6, 1798, 3004, 3046, 3214, 3424, 3749, 3752
Sau96I GGNCC 2 cut(s) 1818, 3692
SduI GDGCHC 4 cut(s) 186, 1072, 1438, 3743
SfaNI GCATC 3 cut(s) 136, 484, 1461
SfuI TTCGAA 2 cut(s) 1896, 2059
SinI GGWCC 2 cut(s) 1818, 3692
SmiMI CAYNNNNRTG 7 cut(s) 393, 600, 1619, 1716, 2030, 3011, 3221
SsiI CCGC 2 cut(s) 438, 1510
SstI GAGCTC 3 cut(s) 186, 1072, 1438
StyI CCWWGG 1 cut(s) 34
TaaI ACNGT 4 cut(s) 1388, 1447, 1606, 3135
TaiI ACGT 5 cut(s) 348, 762, 967, 1457, 3712
TaqII GACCGA 1 cut(s) 1953
TatI WGTACW 3 cut(s) 310, 3470, 3622
TseI GCWGC 8 cut(s) 5, 1797, 3003, 3045, 3213, 3423, 3748, 3751
TspGWI ACGGA 2 cut(s) 327, 3668
Tth111I GACNNNGTC 1 cut(s) 888
Vha464I CTTAAG 6 cut(s) 2006, 2357, 2567, 2777, 2987, 3197
VpaK11BI GGWCC 2 cut(s) 1818, 3692
VspI ATTAAT 6 cut(s) 2307, 2517, 2727, 2937, 3147, 3357
XagI CCTNNNNNAGG 2 cut(s) 630, 1407
XapI RAATTY 8 cut(s) 365, 1377, 1976, 2166, 2376, 2586, 2796, 3476
XbaI TCTAGA 2 cut(s) 1327, 3055
XceI RCATGY 4 cut(s) 601, 729, 1341, 1921
XmnI GAANNNNTTC 1 cut(s) 420
Zsp2I ATGCAT 3 cut(s) 603, 612, 1476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.