RchiOBHm_Chr1g0349221

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000067
Physical Location & Seq
Reverse (-)
42317067 .. 42325706
8640 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57520

Sequence Viewer

Length: 4134 bp
ATGGCTGGTGCTTCTTCATCATCAGGAGGCAGTTTCAGATACGACGTTTTCATCAATTTCAGAGGCGAAGACACCCGTAGGTGTTTCATCTGCCATCTTTACAAAGCTCTGGAACAGAAAGCACTCGACGTCTTCATAGATTCCGAAGAGCTTAGAACAGGCGACGACCTTTCGGCGCTATTCAAAGCCATTGAAGACTCGAGGCTTTCGATTGTTGTTTTCTCTAGACACTATGCCTCTTCCACATGGTGCTTAAAAGAACTGGTCCACATCCTGGATAGGCGGAGCCAGATTGTGGTGCCCATATTCTATCAAGTAAATCCCTCCCATATTTGCAACCTTGAGGAAAGTTTTGCAGAAGCTTTTGCAGAACATGAAAGCAATTCCAAGATCGACAAAGAAGAGTTGGAGAGCTGGAAGTCCAGTTTAAACAGAGCCGCCAATATATCTGGCTACCACTCCACAAATTTTGGGGATGATACCCAGCTTATCGACAGCATTGTAAAAGACATTTTAGAGAAATTGATCCACATCTCACCAAGTAAAACCGATGGCTTGATTGGAATGGATTCTCACATAAATGCAATTGATAGGCTATTACAACTGGGGGTGGATGATTTTCCCATTGTTGGAATCTGGGGTATGGGTGGCATCGGCAAGTCAACCATAGCTAAAGCTTTTTTTGACAGAATTGCTCATCAATTTGAACACAAGTGCTTTCTTGATAATGTAAAGGAGGGCTTCATCACAAAAAATGGTGCAGTACAAATGGTTGAAAAACTTCTATCCAAACTTCTGAAGGTAAAGGATCGAAGCACTTTGGATGGAGGGCTGAATATGATTAGGGAAAGACTAGGTAAGAAAAGAATTCTTCTTGTTCTTGATGATGTGGACAATCTGGACCAAATTGAAACCTTGATTGGAGAGAAACCTACATTTGGTGGAGGGAGCAGAATTATTATAACAACTAGAGATAAGAAGTTACTAGCTGGATATTTGATATACGAGCCCAAGTTGTTCACTAATGAAGAAGCTCTTGCACTCTTTAGCAAGTATGCTTTCAGAACAAACAAACCCTCAGAGAGTTACGATGCTATCGCAAGGCGTGCAGTAAAATACGCCTATGGTCTTCCTTTGGCACTCAAAGTCTTGGGAGCTTTGCTTGACAACAAATCTGTACAGGAGTGGGAATATGAGTTGGAGAAGATAAAGAAAGTTTCAATCACGTCGCTTGATAATAATATTGGACGTGTGCTTTGGGCAAGCTATGATGGATTGGATGAATATCAGAAGAAAATATTTCTAGATATTGCATGTTTCTTTAAGAGAATGGACAAAGATCTTGTAACAAAGTTTCATGAAAGTTGTGGCTTTTTCCCCCAGAATGGACTAAGAGTTTTGGAAGAAAGATCTCTTGTAAGTGTCTCACGTTATGGTGAACTAGAGATGCATGATTTGATACAGGAAGTGGGTAGGGACATCGTCCACCGACAATCTACTGAAGATCCGGGAATGCGTAGTAGATTATGGAATTATGAAGATGTTCACCTAGTGTTAGCTCAAAATACGGCTACGAAAGTTGAAGGCATAATCCTAGACTTGTCAAAGTTAAAAGCGGTATACATAAATGATGAAGTTTTTGTTAGCATGAGTAGACTAAGGCTACTCCAGCTTTCTGAATATCATTATGGAGATGGATATAAATATCATTATGGATGGCAACAAAAACATGCTTATGGAAGTGCAAGGACGCGAGAAGCAGATTATGAACCCTGTGTACAAGACATGAATGAGAACTTGAAGTTTCTTTCAAATGAGTTGAGAGTTCTTGTGTGGCATGGATGCCCCCTAAAGTCTTTCCCAATTAATTTTGTACCAAATAATCTTATTTACATTGACATGTGCAGTAGTCATATTAAACAACTTTGGGAAGGAACCAAGCGTTTGCAAAACTTGGTATACATTAATTTAAGTCATTCAAAGTATCTAATCGAAACCCCCGACTTCACTGAGGCAACACATCTTAAGCAACTGAGGCTTGGAGAATGTACAAGTTTATTGGAGGTTCACCCATCCATTTCAGCTCTTAAAAGCCTCGTCGAGTTGGACCTACGGGGCTGCTCAAATCTCAATAAGTTTCCAAATATATCCGGAAATATGGAGAAGCTAAGTAAACTTTGGTTAGATCAGACTGCAATTACAGAAATACCCTCGTCAATTAATGATCTTTCCGGGCTGCGGGATTTGAGCCTCGCTTGTTGCAGAGAACTCAGGAGTCTTCCGAGCAGCATTCACATGAAATCTCTAAACAAGCTTGATCTATCTGGCTGCTCGAAACTTACGGAGTTTCCAGAGATTTCAAAAGTAATGGAGGTACTAAGGGTGCTTCCATTAGAGGGGACAGCAATTAAAGAACTGCCCTCATCTATTAATAATCTCACGAGGCTTCAGAGCTTAAGTTTGAGAAACTGCACAAGCCTTGTATGTCTTCCGAACAATATCGGCAATATGACATGCCTTCAATATCTCTGTCTGACTGGGTGCAGAGAACTCAGGGGTCTTCCGAGCAGCATTCACATGAAATCTCTGAAATCTCTTGATCTATCTGGCTTCTCGAAACTTACGGAGTTTCTGGAGATTTCAGAAGTAATGGAGGAGCTAAAGGTGCTTCGATTAGACGGGACAGCAATTAAAGAACTACCCTCATCTATTAATAATCTTACGGGGCTTGTTGATTTATATATGAGTAACTGCACAAGCCTTGTCTGTCTTCCGGAAAATATCTGCAATATGGCATGCCTTCAATATCTCTGTCTGACTGGGTGTTCTAACCTTTCAAAGTTGCCCGAGAACTTCGGGAATTTGAAATCATTGTATCGATATGAACTGAAGGGAAGTGGTATAAAACAACTTCCATTCTCTATCTTGTGTAGGTCTTCATTATTATGTAATGGATGTAAAGAGATGACGGAGCCGTTTTCCTCGTGGCCAACATGGAACCACCAAAATCCAAGTTACTCTGTTCTCATCTATCTTGATTTAAGTGACTGCAATCTCTTGGAAATATCGGATGGTATTGCTCATTTATCTTCGTTGCAAACATTAAAGCTGTGTAGAAACGAGAATTTGGAAAGTTTACCTGCAGCAATGAATCGCCTGGGTCATTTAACACTGCTCTATCTGAGAGGTCTGAAGAGACTGAAATCAATACCAGAGCTTTCATCGAGTATACAAGACATAGAAGCCCACAATTGCACATCTTTGGAGACAGTTTCAACACCACAGCCTCCGTACGCTGAGTTCATTTGCTTCCAATTTTTTAATTGCTTCAAGTTGGTAAACAATACCAATATATTTATAAATATTGTGGAAAGTGCTACTCGTAATCAGGATTATTATAAATGCCGTATGACTCTTCCTGGAAGTGATATTCCAGATTGGTTCAACCATCAGAGTGGAGGGTTATCCGTAACTGTGCCGTTACCACCCAATTGGCTCGCAAGCAACTTTCTGGGGTTCGGTATATGCGCCATCAGTAACTTGCGGGGTGCTGATAATAGAAAAACTCTTGAATCTACCACCTGTTGCTGTACCTTAAAAGCAAATGACGGTCAGGACAATTTCTATTTTCCTTTGCTCCCCCCTTTTCGAGGTGGAACTGACGTGCCCCTTGAGTTGGATCATATGTTGCTGGGATATACAAATTTCCGAAGTTTAGGAGAGTGGCATAATGAACGCAAATACACCGAGGCCACATTTAGGATAGTGGCAGAGGACTATGGAGTCATCCGAGAGGATTGGGAAGATTGCACTTGGATCACAAGTTGCGGGGTTCGGTTATTCCACATGAATCCAACCAATGTCGGAGGCCAACGAGCAGAGCCCCTTCGGCGCGTTAACCGCCGTAGAGTTCTACTCCCGTCACTCCGTCTCTCACGGCTCCGACTACGTCACCAACCCCGGAAGCTTCAAGCTCTTCACTCAGTAGTGGACCCCACTCCCTCCAACCGAGGCCCTCGGCGTCCTCGCCATAGTTCACGGCTTCACCAGCGAGACCAGCTCTCTACGGACCTTATTTGCAATTTGGAAAATAGAGAAACAGAGATAAGGAGTAAGAGAAGGAGGAAACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1377

Amino Acids

156.5

Weight (kDa)

7.46

Isoelectric Point (pI)

49.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 14 - 185 3.9e-44 TIR domain
TIR_2 PF13676 16 - 107 1.3e-13 TIR domain
NB-ARC PF00931 195 - 357 3.7e-19 NB-ARC domain
WHD_ROQ1 PF23282 428 - 499 5.9e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 671 - 769 5.3e-06 Leucine-rich repeat region
LRR_13 PF23286 696 - 766 8.4e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 760 - 891 7.3e-12 Leucine-rich repeat region
LRR_13 PF23286 766 - 852 2.7e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 860 - 946 2e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 883 - 940 1e-07 Leucine-rich repeat region
LRR_14 PF23598 1009 - 1081 1.3e-06 Leucine-rich repeat region
C-JID PF20160 1139 - 1284 4.2e-17 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 962, 3359, 3399
AasI GACNNNNNNGTC 1 cut(s) 3782
AatII GACGTC 1 cut(s) 132
Acc36I ACCTGC 1 cut(s) 3148
AccB1I GGYRCC 1 cut(s) 298
AccB7I CCANNNNNTGG 2 cut(s) 274, 295
AccI GTMKAC 4 cut(s) 1620, 1654, 1959, 3229
AccII CGCG 2 cut(s) 1753, 3894
AccIII TCCGGA 2 cut(s) 2150, 2773
AciI CCGC 7 cut(s) 283, 438, 1616, 2239, 3544, 3828, 3901
AclWI GGATC 5 cut(s) 520, 816, 1499, 3687, 3824
AcoI YGGCCR 1 cut(s) 2987
AcsI RAATTY 5 cut(s) 466, 867, 2860, 3124, 3703
AcuI CTGAAG 5 cut(s) 818, 1521, 2432, 2909, 3212
AcyI GRCGYC 2 cut(s) 129, 4021
AdeI CACNNNGTG 2 cut(s) 249, 1552
AfaI GTAC 8 cut(s) 765, 1179, 1779, 1875, 2050, 2376, 3293, 3592
AflII CTTAAG 2 cut(s) 2024, 2455
AflIII ACRYGT 2 cut(s) 1249, 1899
AjiI CACGTC 3 cut(s) 1227, 1250, 3664
AjnI CCWGG 3 cut(s) 273, 3156, 3418
AjuI GAANNNNNNNTTGG 2 cut(s) 903, 935
Alw26I GTCTC 5 cut(s) 1429, 3190, 3260, 3935, 4047
AlwI GGATC 5 cut(s) 520, 816, 1499, 3687, 3824
Ama87I CYCGRG 2 cut(s) 199, 2846
Aor13HI TCCGGA 2 cut(s) 2150, 2773
AoxI GGCC 4 cut(s) 2987, 3750, 3868, 4012
ApeKI GCWGC 6 cut(s) 2118, 2236, 2286, 2328, 2568, 3143
ApoI RAATTY 5 cut(s) 466, 867, 2860, 3124, 3703
ArsI GACNNNNNNTTYG 2 cut(s) 1239, 1271
AseI ATTAAT 5 cut(s) 1866, 1965, 2220, 2430, 2712
Asp700I GAANNNNTTC 3 cut(s) 568, 780, 1542
AspLEI GCGC 3 cut(s) 178, 3530, 3894
AspS9I GGNCC 6 cut(s) 265, 901, 2107, 3991, 4013, 4069
AsuC2I CCSGG 3 cut(s) 1509, 2233, 3961
AsuHPI GGTGA 6 cut(s) 528, 1448, 1538, 2060, 3944, 4037
AvaI CYCGRG 2 cut(s) 199, 2846
AvaII GGWCC 5 cut(s) 265, 901, 2107, 3991, 4069
BaeGI GKGCMC 2 cut(s) 303, 3669
BalI TGGCCA 1 cut(s) 2989
BanI GGYRCC 1 cut(s) 298
BanII GRGCYC 2 cut(s) 1011, 3885
BauI CACGAG 2 cut(s) 2440, 2983
BbsI GAAGAC 9 cut(s) 75, 124, 201, 1121, 2270, 2480, 2552, 2762, 2928
BbvI GCAGC 6 cut(s) 2105, 2223, 2298, 2315, 2580, 3155
BceAI ACGGC 7 cut(s) 1584, 2959, 3390, 3463, 3888, 3953, 4055
BciT130I CCWGG 3 cut(s) 275, 3158, 3420
BcnI CCSGG 3 cut(s) 1509, 2233, 3961
BcoDI GTCTC 5 cut(s) 1429, 3190, 3260, 3935, 4047
BfaI CTAG 9 cut(s) 225, 854, 969, 986, 1304, 1442, 1550, 1595, 4132
BfmI CTRYAG 1 cut(s) 3141
BfoI RGCGCY 1 cut(s) 179
BfrI CTTAAG 2 cut(s) 2024, 2455
BfuAI ACCTGC 1 cut(s) 3148
BglI GCCNNNNNGGC 1 cut(s) 3889
BglII AGATCT 2 cut(s) 1339, 1409
BisI GCNGC 7 cut(s) 438, 2119, 2237, 2287, 2329, 2569, 3144
BlsI GCNGC 7 cut(s) 439, 2120, 2238, 2288, 2330, 2570, 3145
Bme1390I CCNGG 6 cut(s) 275, 1509, 2233, 3158, 3420, 3961
Bme18I GGWCC 5 cut(s) 265, 901, 2107, 3991, 4069
BmeT110I CYCGRG 2 cut(s) 199, 2846
BmgBI CACGTC 3 cut(s) 1227, 1250, 3664
BmgT120I GGNCC 6 cut(s) 265, 901, 2107, 3991, 4013, 4069
BmiI GGNNCC 7 cut(s) 287, 300, 1936, 2973, 2999, 3941, 3993
BmrFI CCNGG 6 cut(s) 275, 1509, 2233, 3158, 3420, 3961
BmrI ACTGGG 3 cut(s) 614, 2547, 2829
BmsI GCATC 4 cut(s) 660, 1081, 1437, 1832
BmuI ACTGGG 3 cut(s) 614, 2547, 2829
BpiI GAAGAC 9 cut(s) 75, 124, 201, 1121, 2270, 2480, 2552, 2762, 2928
BplI GAGNNNNNCTC 4 cut(s) 3900, 3932, 4044, 4076
BpmI CTGGAG 2 cut(s) 1652, 2654
BpuEI CTTGAG 2 cut(s) 362, 3692
BpuMI CCSGG 3 cut(s) 1509, 2233, 3961
Bsa29I ATCGAT 1 cut(s) 2878
BsaBI GATNNNNATC 2 cut(s) 530, 1284
BsaHI GRCGYC 2 cut(s) 129, 4021
BsaI GGTCTC 1 cut(s) 4047
BsaJI CCNNGG 5 cut(s) 3157, 3747, 3959, 4009, 4016
BsaWI WCCGGW 2 cut(s) 2150, 2773
Bse1I ACTGG 5 cut(s) 267, 423, 609, 2542, 2824
Bse3DI GCAATG 1 cut(s) 3153
Bse8I GATNNNNATC 2 cut(s) 530, 1284
BseAI TCCGGA 2 cut(s) 2150, 2773
BseBI CCWGG 3 cut(s) 275, 3158, 3420
BseCI ATCGAT 1 cut(s) 2878
BseDI CCNNGG 5 cut(s) 3157, 3747, 3959, 4009, 4016
BseJI GATNNNNATC 2 cut(s) 530, 1284
BseMI GCAATG 1 cut(s) 3153
BseMII CTCAG 8 cut(s) 1092, 2001, 2024, 2284, 2566, 3173, 3288, 3996
BseNI ACTGG 5 cut(s) 267, 423, 609, 2542, 2824
BseRI GAGGAG 1 cut(s) 2669
BseSI GKGCMC 2 cut(s) 303, 3669
BseXI GCAGC 6 cut(s) 2105, 2223, 2298, 2315, 2580, 3155
BseYI CCCAGC 2 cut(s) 483, 3691
BsgI GTGCAG 6 cut(s) 780, 1128, 1924, 2455, 2563, 2737
Bsh1236I CGCG 2 cut(s) 1753, 3894
BshFI GGCC 4 cut(s) 2989, 3752, 3870, 4014
BshNI GGYRCC 1 cut(s) 298
BshVI ATCGAT 1 cut(s) 2878
BsiHKCI CYCGRG 2 cut(s) 199, 2846
BsiSI CCGG 5 cut(s) 1508, 2151, 2232, 2774, 3961
BsiWI CGTACG 1 cut(s) 3291
BslFI GGGAC 3 cut(s) 1490, 2413, 2695
BsmAI GTCTC 5 cut(s) 1429, 3190, 3260, 3935, 4047
BsmBI CGTCTC 1 cut(s) 3935
BsmFI GGGAC 3 cut(s) 1490, 2413, 2695
BsmI GAATGC 3 cut(s) 1518, 2289, 2571
BsnI GGCC 4 cut(s) 2989, 3752, 3870, 4014
Bso31I GGTCTC 1 cut(s) 4047
BsoBI CYCGRG 2 cut(s) 199, 2846
Bsp1286I GDGCHC 4 cut(s) 303, 1011, 3669, 3885
Bsp13I TCCGGA 2 cut(s) 2150, 2773
Bsp1407I TGTACA 3 cut(s) 1177, 1777, 2048
BspACI CCGC 7 cut(s) 283, 438, 1616, 2239, 3544, 3828, 3901
BspANI GGCC 4 cut(s) 2989, 3752, 3870, 4014
BspCNI CTCAG 8 cut(s) 1091, 2002, 2025, 2283, 2565, 3174, 3289, 3995
BspDI ATCGAT 1 cut(s) 2878
BspEI TCCGGA 2 cut(s) 2150, 2773
BspFNI CGCG 2 cut(s) 1753, 3894
BspHI TCATGA 1 cut(s) 1357
BspLI GGNNCC 7 cut(s) 287, 300, 1936, 2973, 2999, 3941, 3993
BspMAI CTGCAG 1 cut(s) 3145
BspMI ACCTGC 1 cut(s) 3148
BspPI GGATC 5 cut(s) 520, 816, 1499, 3687, 3824
BspQI GCTCTTC 2 cut(s) 141, 3981
BspT107I GGYRCC 1 cut(s) 298
BspTI CTTAAG 2 cut(s) 2024, 2455
BspTNI GGTCTC 1 cut(s) 4047
BsrDI GCAATG 1 cut(s) 3153
BsrGI TGTACA 3 cut(s) 1177, 1777, 2048
BsrI ACTGG 5 cut(s) 267, 423, 609, 2542, 2824
BssECI CCNNGG 5 cut(s) 3157, 3747, 3959, 4009, 4016
BssNAI GTATAC 3 cut(s) 1621, 1960, 3230
BssNI GRCGYC 2 cut(s) 129, 4021
BssSI CACGAG 2 cut(s) 2440, 2983
Bst1107I GTATAC 3 cut(s) 1621, 1960, 3230
Bst2BI CACGAG 2 cut(s) 2440, 2983
Bst2UI CCWGG 3 cut(s) 275, 3158, 3420
Bst4CI ACNGT 3 cut(s) 3271, 3475, 3611
Bst6I CTCTTC 6 cut(s) 141, 244, 396, 3188, 3420, 3981
BstACI GRCGYC 2 cut(s) 129, 4021
BstAFI CTTAAG 2 cut(s) 2024, 2455
BstAPI GCANNNNNTGC 1 cut(s) 1106
BstAUI TGTACA 3 cut(s) 1177, 1777, 2048
BstC8I GCNNGC 5 cut(s) 1107, 1264, 2797, 3498, 3502
BstENI CCTNNNNNAGG 1 cut(s) 3648
BstFNI CGCG 2 cut(s) 1753, 3894
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 3 cut(s) 178, 3530, 3894
BstMAI GTCTC 5 cut(s) 1429, 3190, 3260, 3935, 4047
BstMWI GCNNNNNNNGC 8 cut(s) 1106, 1669, 2035, 2665, 3889, 3900, 4048, 4057
BstNI CCWGG 3 cut(s) 275, 3158, 3420
BstNSI RCATGY 5 cut(s) 1317, 1733, 1903, 2517, 2799
BstSCI CCNGG 6 cut(s) 273, 1507, 2231, 3156, 3418, 3959
BstSFI CTRYAG 1 cut(s) 3141
BstSLI GKGCMC 2 cut(s) 303, 3669
BstUI CGCG 2 cut(s) 1753, 3894
BstV1I GCAGC 6 cut(s) 2105, 2223, 2298, 2315, 2580, 3155
BstV2I GAAGAC 9 cut(s) 75, 124, 201, 1121, 2270, 2480, 2552, 2762, 2928
BstX2I RGATCY 3 cut(s) 1339, 1409, 1504
BstXI CCANNNNNNTGG 2 cut(s) 3455, 3492
BstYI RGATCY 3 cut(s) 1339, 1409, 1504
BstZ17I GTATAC 3 cut(s) 1621, 1960, 3230
Bsu15I ATCGAT 1 cut(s) 2878
BsuRI GGCC 4 cut(s) 2989, 3752, 3870, 4014
BsuTUI ATCGAT 1 cut(s) 2878
BtrI CACGTC 3 cut(s) 1227, 1250, 3664
BtsI GCAGTG 1 cut(s) 3170
BtsIMutI CAGTG 2 cut(s) 2007, 3170
BveI ACCTGC 1 cut(s) 3148
Cac8I GCNNGC 5 cut(s) 1107, 1264, 2797, 3498, 3502
CciI TCATGA 1 cut(s) 1357
CfoI GCGC 3 cut(s) 178, 3530, 3894
Cfr13I GGNCC 6 cut(s) 265, 901, 2107, 3991, 4013, 4069
ClaI ATCGAT 1 cut(s) 2878
CseI GACGC 2 cut(s) 1759, 4010
Csp6I GTAC 8 cut(s) 764, 1178, 1778, 1874, 2049, 2375, 3292, 3591
CspCI CAANNNNNGTGG 2 cut(s) 451, 486
CviQI GTAC 8 cut(s) 764, 1178, 1778, 1874, 2049, 2375, 3292, 3591
DraI TTTAAA 1 cut(s) 429
DraIII CACNNNGTG 2 cut(s) 249, 1552
DrdI GACNNNNNNGTC 1 cut(s) 3782
DseDI GACNNNNNNGTC 1 cut(s) 3782
EaeI YGGCCR 1 cut(s) 2987
Eam1104I CTCTTC 6 cut(s) 141, 244, 396, 3188, 3420, 3981
EarI CTCTTC 6 cut(s) 141, 244, 396, 3188, 3420, 3981
EciI GGCGGA 1 cut(s) 298
Eco24I GRGCYC 2 cut(s) 1011, 3885
Eco31I GGTCTC 1 cut(s) 4047
Eco47I GGWCC 5 cut(s) 265, 901, 2107, 3991, 4069
Eco57I CTGAAG 5 cut(s) 818, 1521, 2432, 2909, 3212
Eco88I CYCGRG 2 cut(s) 199, 2846
EcoNI CCTNNNNNAGG 1 cut(s) 3648
EcoO109I RGGNCCY 1 cut(s) 4013
EcoRI GAATTC 1 cut(s) 867
EcoRII CCWGG 3 cut(s) 273, 3156, 3418
EcoT22I ATGCAT 1 cut(s) 1452
EcoT38I GRGCYC 2 cut(s) 1011, 3885
Esp3I CGTCTC 1 cut(s) 3935
FalI AAGNNNNNCTT 4 cut(s) 725, 757, 1020, 1052
FaqI GGGAC 3 cut(s) 1490, 2413, 2695
FauI CCCGC 3 cut(s) 2232, 3537, 3821
FauNDI CATATG 1 cut(s) 3684
FblI GTMKAC 4 cut(s) 1620, 1654, 1959, 3229
Fnu4HI GCNGC 7 cut(s) 438, 2119, 2237, 2287, 2329, 2569, 3144
FriOI GRGCYC 2 cut(s) 1011, 3885
Fsp4HI GCNGC 7 cut(s) 438, 2119, 2237, 2287, 2329, 2569, 3144
FspBI CTAG 9 cut(s) 225, 854, 969, 986, 1304, 1442, 1550, 1595, 4132
GlaI GCGC 3 cut(s) 177, 3529, 3893
GluI GCNGC 7 cut(s) 438, 2119, 2237, 2287, 2329, 2569, 3144
GsaI CCCAGC 2 cut(s) 487, 3695
GsuI CTGGAG 2 cut(s) 1652, 2654
HaeII RGCGCY 1 cut(s) 179
HaeIII GGCC 4 cut(s) 2989, 3752, 3870, 4014
HapII CCGG 5 cut(s) 1508, 2151, 2232, 2774, 3961
HgaI GACGC 2 cut(s) 1759, 4010
HhaI GCGC 3 cut(s) 178, 3530, 3894
Hin1I GRCGYC 2 cut(s) 129, 4021
Hin6I GCGC 3 cut(s) 176, 3528, 3892
HinP1I GCGC 3 cut(s) 176, 3528, 3892
HincII GTYRAC 2 cut(s) 663, 3898
HindII GTYRAC 2 cut(s) 663, 3898
HindIII AAGCTT 4 cut(s) 360, 675, 2312, 3965
HpaI GTTAAC 1 cut(s) 3898
HpaII CCGG 5 cut(s) 1508, 2151, 2232, 2774, 3961
HphI GGTGA 6 cut(s) 528, 1448, 1538, 2060, 3944, 4037
Hpy99I CGWCG 5 cut(s) 47, 131, 167, 1231, 2102
HpyAV CCTTC 8 cut(s) 793, 1577, 1925, 2528, 2810, 2884, 3896, 4113
HpyCH4III ACNGT 3 cut(s) 3271, 3475, 3611
HpyCH4IV ACGT 7 cut(s) 45, 129, 1226, 1249, 1429, 3663, 3949
HpyF10VI GCNNNNNNNGC 8 cut(s) 1106, 1669, 2035, 2665, 3889, 3900, 4048, 4057
HpySE526I ACGT 7 cut(s) 45, 129, 1226, 1249, 1429, 3663, 3949
Hsp92I GRCGYC 2 cut(s) 129, 4021
HspAI GCGC 3 cut(s) 176, 3528, 3892
Kpn2I TCCGGA 2 cut(s) 2150, 2773
KspAI GTTAAC 1 cut(s) 3898
LguI GCTCTTC 2 cut(s) 141, 3981
LmnI GCTCC 7 cut(s) 285, 948, 1154, 2656, 2971, 3642, 3945
Lsp1109I GCAGC 6 cut(s) 2105, 2223, 2298, 2315, 2580, 3155
LweI GCATC 4 cut(s) 660, 1081, 1437, 1832
MaeI CTAG 9 cut(s) 225, 854, 969, 986, 1304, 1442, 1550, 1595, 4132
MaeII ACGT 7 cut(s) 45, 129, 1226, 1249, 1429, 3663, 3949
MfeI CAATTG 3 cut(s) 585, 3250, 3490
MflI RGATCY 3 cut(s) 1339, 1409, 1504
MhlI GDGCHC 4 cut(s) 303, 1011, 3669, 3885
MlsI TGGCCA 1 cut(s) 2989
MluNI TGGCCA 1 cut(s) 2989
MlyI GAGTC 4 cut(s) 191, 2284, 3406, 3792
MmeI TCCRAC 9 cut(s) 387, 610, 1179, 2085, 3657, 3844, 3878, 3967, 4029
Mox20I TGGCCA 1 cut(s) 2989
Mph1103I ATGCAT 1 cut(s) 1452
MroI TCCGGA 2 cut(s) 2150, 2773
MroXI GAANNNNTTC 3 cut(s) 568, 780, 1542
MscI TGGCCA 1 cut(s) 2989
MslI CAYNNNNRTG 7 cut(s) 579, 1734, 1898, 2294, 2576, 2944, 3453
Msp20I TGGCCA 1 cut(s) 2989
MspCI CTTAAG 2 cut(s) 2024, 2455
MspI CCGG 5 cut(s) 1508, 2151, 2232, 2774, 3961
MspR9I CCNGG 6 cut(s) 275, 1509, 2233, 3158, 3420, 3961
MssI GTTTAAAC 1 cut(s) 429
MunI CAATTG 3 cut(s) 585, 3250, 3490
Mva1269I GAATGC 3 cut(s) 1518, 2289, 2571
MvaI CCWGG 3 cut(s) 275, 3158, 3420
MvnI CGCG 2 cut(s) 1753, 3894
MwoI GCNNNNNNNGC 8 cut(s) 1106, 1669, 2035, 2665, 3889, 3900, 4048, 4057
NciI CCSGG 3 cut(s) 1509, 2233, 3961
NdeI CATATG 1 cut(s) 3684
NlaIV GGNNCC 7 cut(s) 287, 300, 1936, 2973, 2999, 3941, 3993
NmeAIII GCCGAG 1 cut(s) 3997
NmuCI GTSAC 3 cut(s) 3044, 3921, 3950
NsiI ATGCAT 1 cut(s) 1452
NspI RCATGY 5 cut(s) 1317, 1733, 1903, 2517, 2799
PaeI GCATGC 1 cut(s) 2799
PaeR7I CTCGAG 1 cut(s) 199
PagI TCATGA 1 cut(s) 1357
PciI ACATGT 1 cut(s) 1899
PciSI GCTCTTC 2 cut(s) 141, 3981
PcsI WCGNNNNNNNCGW 2 cut(s) 206, 1998
PctI GAATGC 3 cut(s) 1518, 2289, 2571
PdmI GAANNNNTTC 3 cut(s) 568, 780, 1542
PfeI GAWTC 6 cut(s) 140, 569, 633, 3151, 3572, 3850
Pfl23II CGTACG 1 cut(s) 3291
PflFI GACNNNGTC 2 cut(s) 1481, 3948
PflMI CCANNNNNTGG 2 cut(s) 274, 295
PfoI TCCNGGA 3 cut(s) 273, 1507, 3418
PkrI GCNGC 7 cut(s) 439, 2120, 2238, 2288, 2330, 2570, 3145
PleI GAGTC 4 cut(s) 191, 2283, 3406, 3791
PmeI GTTTAAAC 1 cut(s) 429
PpsI GAGTC 4 cut(s) 191, 2283, 3406, 3791
PscI ACATGT 1 cut(s) 1899
PshBI ATTAAT 5 cut(s) 1866, 1965, 2220, 2430, 2712
PsiI TTATAA 3 cut(s) 962, 3359, 3399
Psp6I CCWGG 3 cut(s) 273, 3156, 3418
PspFI CCCAGC 2 cut(s) 483, 3691
PspGI CCWGG 3 cut(s) 273, 3156, 3418
PspLI CGTACG 1 cut(s) 3291
PspN4I GGNNCC 7 cut(s) 287, 300, 1936, 2973, 2999, 3941, 3993
PspPI GGNCC 6 cut(s) 265, 901, 2107, 3991, 4013, 4069
PspXI VCTCGAGB 1 cut(s) 199
PsrI GAACNNNNNNTAC 4 cut(s) 1761, 1793, 3284, 3316
PstI CTGCAG 1 cut(s) 3145
PsuI RGATCY 3 cut(s) 1339, 1409, 1504
PsyI GACNNNGTC 2 cut(s) 1481, 3948
RsaI GTAC 8 cut(s) 765, 1179, 1779, 1875, 2050, 2376, 3293, 3592
RsaNI GTAC 8 cut(s) 764, 1178, 1778, 1874, 2049, 2375, 3292, 3591
RseI CAYNNNNRTG 7 cut(s) 579, 1734, 1898, 2294, 2576, 2944, 3453
SapI GCTCTTC 2 cut(s) 141, 3981
SatI GCNGC 7 cut(s) 438, 2119, 2237, 2287, 2329, 2569, 3144
Sau96I GGNCC 6 cut(s) 265, 901, 2107, 3991, 4013, 4069
SchI GAGTC 4 cut(s) 191, 2284, 3406, 3792
ScrFI CCNGG 6 cut(s) 275, 1509, 2233, 3158, 3420, 3961
SduI GDGCHC 4 cut(s) 303, 1011, 3669, 3885
SfaNI GCATC 4 cut(s) 660, 1081, 1437, 1832
SfcI CTRYAG 1 cut(s) 3141
Sfr274I CTCGAG 1 cut(s) 199
SinI GGWCC 5 cut(s) 265, 901, 2107, 3991, 4069
SlaI CTCGAG 1 cut(s) 199
SmiMI CAYNNNNRTG 7 cut(s) 579, 1734, 1898, 2294, 2576, 2944, 3453
SmlI CTYRAG 5 cut(s) 199, 341, 2024, 2455, 3671
SmoI CTYRAG 5 cut(s) 199, 341, 2024, 2455, 3671
SphI GCATGC 1 cut(s) 2799
SsiI CCGC 7 cut(s) 283, 438, 1616, 2239, 3544, 3828, 3901
SspI AATATT 3 cut(s) 1243, 1299, 3364
SspMI CTAG 9 cut(s) 225, 854, 969, 986, 1304, 1442, 1550, 1595, 4132
StyD4I CCNGG 6 cut(s) 273, 1507, 2231, 3156, 3418, 3959
TaaI ACNGT 3 cut(s) 3271, 3475, 3611
TaiI ACGT 7 cut(s) 48, 132, 1229, 1252, 1432, 3666, 3952
TatI WGTACW 4 cut(s) 763, 1177, 1777, 2048
TauI GCSGC 1 cut(s) 440
TfiI GAWTC 6 cut(s) 140, 569, 633, 3151, 3572, 3850
TscAI CASTG 2 cut(s) 2014, 3177
TseFI GTSAC 3 cut(s) 3044, 3921, 3950
TseI GCWGC 6 cut(s) 2118, 2236, 2286, 2328, 2568, 3143
Tsp45I GTSAC 3 cut(s) 3044, 3921, 3950
TspGWI ACGGA 7 cut(s) 2357, 2639, 2984, 3279, 3457, 3917, 4082
TspRI CASTG 2 cut(s) 2014, 3177
Tth111I GACNNNGTC 2 cut(s) 1481, 3948
Van91I CCANNNNNTGG 2 cut(s) 274, 295
Vha464I CTTAAG 2 cut(s) 2024, 2455
VpaK11BI GGWCC 5 cut(s) 265, 901, 2107, 3991, 4069
VspI ATTAAT 5 cut(s) 1866, 1965, 2220, 2430, 2712
XagI CCTNNNNNAGG 1 cut(s) 3648
XapI RAATTY 5 cut(s) 466, 867, 2860, 3124, 3703
XbaI TCTAGA 2 cut(s) 224, 1303
XceI RCATGY 5 cut(s) 1317, 1733, 1903, 2517, 2799
XhoI CTCGAG 1 cut(s) 199
XmiI GTMKAC 4 cut(s) 1620, 1654, 1959, 3229
XmnI GAANNNNTTC 3 cut(s) 568, 780, 1542
XspI CTAG 9 cut(s) 225, 854, 969, 986, 1304, 1442, 1550, 1595, 4132
ZraI GACGTC 1 cut(s) 130
Zsp2I ATGCAT 1 cut(s) 1452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.