MD07G1261100.v1.1

cellular transition metal ion homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000067
Physical Location & Seq
Forward (+)
32679484 .. 32683843
4360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1261100.v1.1.491

Sequence Viewer

Length: 2988 bp
ATGCATGATTTACTAGAGGAAATGGGTCGGGAAATAGTTCGCCAAGAATCTATCGAAGAGCCTGGAAGACGAAGTAGGTTGTGGAGTTATGAAGATGTCCATCATGTGCTAACTCAAAATAAGGCTACAAAAGTAGTTGAAAGCATAATCCTGGATTTATCGAACTCAGATGAGGTATGCTTAAATGCTGAAGCATTTGTTAGTATGACCCAACTAAGACTTCTCAAAATCAGTCACGAGAAGTACAATTACTGCAAACAGCACCTGATTGGGCACTTCAAGTTTCTTTCTCGTGAGTTGAGGTGTCTCTCCTGGCTTCGATTCCCCCTTAAGTCTTTGCCATCCAACTTTCAATTCAAAAATCTTGTTGACCTTGACATGCAATACAGTCTCATTGAGCAACTTTGGGAAGGAATCCAGACGCTGGAAAAGTTGAAATTCATCAATTTAAGTTATTGTCAATACCTTAAGGAGACCCCAGACTTCACAAAGGTGCCAAATCTTGAGAGGCTAATCCTTCAAAATTGTATAAGTTTAGTTGAGGTTCACCCATCTATTTGGACTCTTACAAAACTTGTTTTATTGAATTTGAATGGGTGCAAAGAACTTAAGATTCTAGCCAGCAACATTCATATGCGCTCTCTTAAAACCCTTGATCTTTCTGGTTGCTCAAAGCTTGACAAGTTTCCTGAGATTTTAGAAGTTATGGAGGAACTATCACATCTTAATTTATCCGGGTCAAAAATTAAAAAATTGCCCTCGTCTATCGATAATCTCACGGGGTTGAACTATTTGAACCTAAAAGATTGCAAGGAACTTAAGAGTCTTCCAAGCAGCATTCATATGAAATCTCTCAAACACCTTAATCTTTTTGGCTGCTCGAGTCTTGAGATGTTTCCAGAGATTTCAAAAGTTATGGAGAAACTATCATGGCTTGATTTGTCTGGGTTAAAAATTAAAGAACTGCCCTCGTCGATTAATAATCTCATGGGGTTGAGTTCTTTGTACCTAAAAGATTGCAAGGAACTTAAGAGTCTTCCAAGCAGCATTCGTATGAAATCTCTCAAAACCTTTAATCTTGATGGTTGCTCGAGTCTGGAGATGTTTCCAGAGATTTCAGAAGTGATTGAGGGGTTAAAAGCTCTTAATTTATCTGGGTCAAAAATTAAAGAACTGCCCTCGTCGATTAATAATCTCACGGGGTTGAGATATTTGGACCTAAAAGATTGCAAGGAACTTAAGAGTCTTCCAAGCAGCATTCCTATGAAATCTCTCAAAACCTTTTATCTTTGTGGCTGCTCGAGTCTTGAGATGTTTCCAGAGATTTCAGAAGTGATGGAGAAACTATCATTGCTTGATTTGTCCGGGTCAAAAATTAAAGAACTACGCTTGTCGATTAATAATCTCACGGAGTTGAGATATTTGGACCTAAAAGATTGCAAGGAACTTAAGAGTCTTCCCAACATCATTCGTATGAAATCTCTCAACACCCTTGATCTTTGTGGCTGCTCGAGTCTTAAGATGTTTCCAGAGATTTCAGAAGTTATGGAGAAACTATCACGACTTAGTGTGTCCGAGTCAAAAATTAAAGAACTACCCTCGTCGATTAATAATCTCACGGGGTTGAAGTATTTGGACCTAAAAGATTGCAAGGAACTTAAGAGTCTTCCAAGCAGCATTCATATGAAATCTCTCAAAACCCTTAATCTTTGTGGTTGCTCGAGTCTTGAGATCTTTCCAGAGATTTCAGAAGTGATGAAGAAACTATCATGGCTTGATTTGTCTGGGTCAAAAATTAAAGAATTGCCCTCGTCGATTAATAATCTCACGGGATTGAAGTATTTGGACCTAAAAGATTGCAAGGAACTTAAGAGTCTTCCAAGCAACATTCATATAAAATCTCTCAAAACCCTTGATCTTTGTGGCTGCTCGAGTCTTGAGATCTTTCCAGAGATTTCTGAAGTGATGGAGAAACTATCATTGCTTGATTTGTCTGGGTCAAAAATTAAAGAACTATGCTTGTCGATTAATAATCTCACGGGGTTGAGATATTTGGACCTAAAAGATTGCAAGGAACTTAAGAGTCTTCCAAGCAGCTTTCCTATGAAATCTCTCAAAACCCTTGATCTTTGTGGCTGCTCGAGTCTTGAAATGTTTCCAGAGATTTCAGAAGTGAAGGACAACAAAGACGACGGCGGCGATGCCAAGAATAAGCCGCTGGAGAAATCCAATTACGAAAAAACCAAAGAGCATCCGGTGAGGATGGCGGTTCTGAAGCTGAACCTCCACTGCCAGGGATGCATCCATAAGATCCACAGGATTGTCACCAAGACGAAAGGGTTCACCTACATGAGCATCGACAAGGAGAATGAGCTGCTGATGGTGAAGGGCTCGATGAACATGAAGGTTCTGGCCGAGAGCTTGAAGGAGAAGCTGAGGAGAAAGGCAATGGCCAAATGGACAGAACATGTCAGGAGAGTAATCTCTGAGAATGGGATTGAAGGACCCCATGAAGATGGTCACAGCTGGAGAAAATATGGGCAGAAAGACATCCTAGGAGCCAAATATCCAAGAAGCTATTACAGATGCACGTACCTGTTCACGCAAAAATGTAGGGCCAGAAAGAAAGTGCAAAGATCAGATGAAGACCCCACCGTCTTCGAAATCACATACGAAGGGAAGCATACATGTTCTCATGGCGGCAGTTCAGTTCCACCGCCACCATCACCAGAAAAGCAAGAACGAAAACGACACAATCATAACAATACTTATCAACAACAGCAGTCTCAAGGAAACCAAATGAGCTTCCCAACTAATCTGAGAGTCAATACTGAGAACTTAGACGACAGAGAGAACACAGCATCTCCATTCTCTTTTACCTCATCTCCATTCGGAAGTATCAGCGTTGACGCCTTCCTATCTTCGATGCTTGATGATAAAAGTCTTTTTGCCAGTCCACCAGAAAAGTCATTGGTTCTCATTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 ko04978 map00940 map01100 map01110 map04978
Pfam Domains
Protein Families
HMA

Protein Analysis

996

Amino Acids

113.19

Weight (kDa)

8.64

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_3 PF07725 121 - 140 5e-07 Leucine Rich Repeat
LRR_14 PF23598 182 - 304 1.2e-07 Leucine-rich repeat region
LRR_13 PF23286 213 - 282 1.2e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 283 - 364 4.1e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 353 - 436 4.8e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 356 - 455 1.7e-06 Leucine-rich repeat region
LRR_14 PF23598 428 - 550 4.9e-09 Leucine-rich repeat region
LRR_13 PF23286 493 - 562 8.2e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 542 - 720 1.2e-10 Leucine-rich repeat region
LRR_13 PF23286 562 - 631 9.4e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_8 PF13855 564 - 620 5.7e-06 Leucine rich repeat
LRR_13 PF23286 632 - 716 6.6e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
WRKY PF03106 838 - 897 1.3e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2654
AccB1I GGYRCC 1 cut(s) 493
AccB7I CCANNNNNTGG 1 cut(s) 424
AciI CCGC 5 cut(s) 2196, 2216, 2267, 2700, 2717
AclWI GGATC 1 cut(s) 2305
AcoI YGGCCR 2 cut(s) 2412, 2451
AcsI RAATTY 2 cut(s) 437, 586
AcuI CTGAAG 3 cut(s) 210, 1980, 2294
AcyI GRCGYC 1 cut(s) 2910
AfaI GTAC 3 cut(s) 245, 1007, 2594
AfiI CCNNNNNNNGG 2 cut(s) 424, 2293
AflIII ACRYGT 2 cut(s) 2467, 2687
AjnI CCWGG 4 cut(s) 61, 150, 311, 2292
AjuI GAANNNNNNNTTGG 4 cut(s) 204, 236, 338, 370
AleI CACNNNNGTG 1 cut(s) 491
Alw26I GTCTC 4 cut(s) 311, 395, 467, 2790
AlwI GGATC 1 cut(s) 2305
AlwNI CAGNNNCTG 2 cut(s) 265, 424
Ama87I CYCGRG 7 cut(s) 880, 1090, 1300, 1510, 1720, 1930, 2140
AoxI GGCC 3 cut(s) 2412, 2451, 2616
ApoI RAATTY 2 cut(s) 437, 586
AseI ATTAAT 6 cut(s) 978, 1188, 1398, 1608, 1818, 2028
Asp700I GAANNNNTTC 3 cut(s) 36, 2154, 2339
AspA2I CCTAGG 1 cut(s) 2554
AspLEI GCGC 1 cut(s) 639
AspS9I GGNCC 7 cut(s) 1216, 1426, 1636, 1846, 2056, 2504, 2616
AsuC2I CCSGG 2 cut(s) 736, 1366
AsuHPI GGTGA 6 cut(s) 539, 2269, 2317, 2335, 2395, 2718
AsuII TTCGAA 1 cut(s) 2661
AvaI CYCGRG 7 cut(s) 880, 1090, 1300, 1510, 1720, 1930, 2140
AvaII GGWCC 6 cut(s) 1216, 1426, 1636, 1846, 2056, 2504
AvrII CCTAGG 1 cut(s) 2554
BaeGI GKGCMC 1 cut(s) 276
BalI TGGCCA 1 cut(s) 2453
BanI GGYRCC 1 cut(s) 493
BanII GRGCYC 1 cut(s) 2393
BarI GAAGNNNNNNTAC 2 cut(s) 233, 265
BauI CACGAG 2 cut(s) 236, 291
BbvCI CCTCAGC 1 cut(s) 2435
BceAI ACGGC 1 cut(s) 2209
BciT130I CCWGG 4 cut(s) 63, 152, 313, 2294
BcnI CCSGG 2 cut(s) 736, 1366
BcoDI GTCTC 4 cut(s) 311, 395, 467, 2790
BfaI CTAG 3 cut(s) 14, 617, 2555
BglII AGATCT 2 cut(s) 1731, 1941
BlnI CCTAGG 1 cut(s) 2554
Bme1390I CCNGG 6 cut(s) 63, 152, 313, 736, 1366, 2294
Bme18I GGWCC 6 cut(s) 1216, 1426, 1636, 1846, 2056, 2504
BmeT110I CYCGRG 7 cut(s) 880, 1090, 1300, 1510, 1720, 1930, 2140
BmgT120I GGNCC 7 cut(s) 1216, 1426, 1636, 1846, 2056, 2504, 2616
BmiI GGNNCC 3 cut(s) 495, 2506, 2560
BmrFI CCNGG 6 cut(s) 63, 152, 313, 736, 1366, 2294
BmsI GCATC 8 cut(s) 2191, 2260, 2288, 2310, 2364, 2576, 2870, 2916
BpmI CTGGAG 3 cut(s) 1118, 2240, 2548
Bpu10I CCTNAGC 1 cut(s) 2435
Bpu14I TTCGAA 1 cut(s) 2661
BpuEI CTTGAG 6 cut(s) 524, 908, 1328, 1748, 1958, 2772
BpuMI CCSGG 2 cut(s) 736, 1366
Bsa29I ATCGAT 1 cut(s) 768
BsaAI YACGTR 1 cut(s) 2592
BsaBI GATNNNNATC 1 cut(s) 99
BsaHI GRCGYC 1 cut(s) 2910
BsaI GGTCTC 1 cut(s) 467
BsaJI CCNNGG 2 cut(s) 2293, 2554
BsaWI WCCGGW 1 cut(s) 2254
BsaXI ACNNNNNCTCC 4 cut(s) 2848, 2869, 2878, 2899
Bsc4I CCNNNNNNNGG 2 cut(s) 424, 2293
Bse1I ACTGG 1 cut(s) 2952
Bse3DI GCAATG 3 cut(s) 1349, 1979, 2454
Bse8I GATNNNNATC 1 cut(s) 99
BseBI CCWGG 4 cut(s) 63, 152, 313, 2294
BseCI ATCGAT 1 cut(s) 768
BseDI CCNNGG 2 cut(s) 2293, 2554
BseGI GGATG 6 cut(s) 341, 2251, 2268, 2301, 2303, 2550
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 2 cut(s) 424, 2293
BseMI GCAATG 3 cut(s) 1349, 1979, 2454
BseMII CTCAG 6 cut(s) 180, 681, 2426, 2478, 2810, 2823
BseNI ACTGG 1 cut(s) 2952
BseRI GAGGAG 1 cut(s) 2452
BseSI GKGCMC 1 cut(s) 276
BshFI GGCC 3 cut(s) 2414, 2453, 2618
BshNI GGYRCC 1 cut(s) 493
BshVI ATCGAT 1 cut(s) 768
BsiHKCI CYCGRG 7 cut(s) 880, 1090, 1300, 1510, 1720, 1930, 2140
BsiSI CCGG 3 cut(s) 735, 1365, 2255
BslI CCNNNNNNNGG 2 cut(s) 424, 2293
BsmAI GTCTC 4 cut(s) 311, 395, 467, 2790
BsmI GAATGC 4 cut(s) 837, 1047, 1257, 1677
BsnI GGCC 3 cut(s) 2414, 2453, 2618
Bso31I GGTCTC 1 cut(s) 467
BsoBI CYCGRG 7 cut(s) 880, 1090, 1300, 1510, 1720, 1930, 2140
Bsp119I TTCGAA 1 cut(s) 2661
Bsp1286I GDGCHC 2 cut(s) 276, 2393
Bsp143I GATC 8 cut(s) 655, 1495, 1731, 1915, 1941, 2125, 2310, 2636
BspACI CCGC 5 cut(s) 2196, 2216, 2267, 2700, 2717
BspANI GGCC 3 cut(s) 2414, 2453, 2618
BspCNI CTCAG 6 cut(s) 179, 682, 2427, 2479, 2811, 2824
BspDI ATCGAT 1 cut(s) 768
BspLI GGNNCC 3 cut(s) 495, 2506, 2560
BspPI GGATC 1 cut(s) 2305
BspQI GCTCTTC 1 cut(s) 51
BspT104I TTCGAA 1 cut(s) 2661
BspT107I GGYRCC 1 cut(s) 493
BspTNI GGTCTC 1 cut(s) 467
BsrDI GCAATG 3 cut(s) 1349, 1979, 2454
BsrI ACTGG 1 cut(s) 2952
BssECI CCNNGG 2 cut(s) 2293, 2554
BssMI GATC 8 cut(s) 655, 1495, 1731, 1915, 1941, 2125, 2310, 2636
BssNI GRCGYC 1 cut(s) 2910
BssSI CACGAG 2 cut(s) 236, 291
BssT1I CCWWGG 1 cut(s) 2554
Bst2BI CACGAG 2 cut(s) 236, 291
Bst2UI CCWGG 4 cut(s) 63, 152, 313, 2294
Bst4CI ACNGT 2 cut(s) 389, 2656
Bst6I CTCTTC 1 cut(s) 51
BstACI GRCGYC 1 cut(s) 2910
BstBAI YACGTR 1 cut(s) 2592
BstBI TTCGAA 1 cut(s) 2661
BstC8I GCNNGC 1 cut(s) 622
BstDEI CTNAG 9 cut(s) 166, 215, 690, 1565, 2435, 2487, 2819, 2832, 2839
BstF5I GGATG 6 cut(s) 341, 2251, 2268, 2301, 2303, 2550
BstHHI GCGC 1 cut(s) 639
BstKTI GATC 8 cut(s) 658, 1498, 1734, 1918, 1944, 2128, 2313, 2639
BstMAI GTCTC 4 cut(s) 311, 395, 467, 2790
BstMBI GATC 8 cut(s) 655, 1495, 1731, 1915, 1941, 2125, 2310, 2636
BstMWI GCNNNNNNNGC 1 cut(s) 2298
BstNI CCWGG 4 cut(s) 63, 152, 313, 2294
BstNSI RCATGY 3 cut(s) 382, 2471, 2691
BstSCI CCNGG 6 cut(s) 61, 150, 311, 734, 1364, 2292
BstSLI GKGCMC 1 cut(s) 276
BstX2I RGATCY 3 cut(s) 1731, 1941, 2310
BstXI CCANNNNNNTGG 2 cut(s) 558, 2516
BstYI RGATCY 3 cut(s) 1731, 1941, 2310
Bsu15I ATCGAT 1 cut(s) 768
BsuRI GGCC 3 cut(s) 2414, 2453, 2618
BsuTUI ATCGAT 1 cut(s) 768
BtgZI GCGATG 1 cut(s) 2214
BtsCI GGATG 6 cut(s) 341, 2251, 2268, 2301, 2303, 2550
BtsI GCAGTG 1 cut(s) 2287
BtsIMutI CAGTG 1 cut(s) 2287
Cac8I GCNNGC 1 cut(s) 622
CaiI CAGNNNCTG 2 cut(s) 265, 424
CfoI GCGC 1 cut(s) 639
Cfr13I GGNCC 7 cut(s) 1216, 1426, 1636, 1846, 2056, 2504, 2616
ClaI ATCGAT 1 cut(s) 768
CseI GACGC 2 cut(s) 430, 2918
Csp6I GTAC 3 cut(s) 244, 1006, 2593
CviQI GTAC 3 cut(s) 244, 1006, 2593
DdeI CTNAG 9 cut(s) 166, 215, 690, 1565, 2435, 2487, 2819, 2832, 2839
DpnI GATC 8 cut(s) 657, 1497, 1733, 1917, 1943, 2127, 2312, 2638
DpnII GATC 8 cut(s) 655, 1495, 1731, 1915, 1941, 2125, 2310, 2636
DrdI GACNNNNNNGTC 1 cut(s) 2654
DseDI GACNNNNNNGTC 1 cut(s) 2654
EaeI YGGCCR 2 cut(s) 2412, 2451
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 2554
Eco24I GRGCYC 1 cut(s) 2393
Eco31I GGTCTC 1 cut(s) 467
Eco47I GGWCC 6 cut(s) 1216, 1426, 1636, 1846, 2056, 2504
Eco57I CTGAAG 3 cut(s) 210, 1980, 2294
Eco88I CYCGRG 7 cut(s) 880, 1090, 1300, 1510, 1720, 1930, 2140
EcoO109I RGGNCCY 1 cut(s) 2504
EcoRII CCWGG 4 cut(s) 61, 150, 311, 2292
EcoT14I CCWWGG 1 cut(s) 2554
EcoT22I ATGCAT 2 cut(s) 6, 2303
EcoT38I GRGCYC 1 cut(s) 2393
ErhI CCWWGG 1 cut(s) 2554
FauNDI CATATG 3 cut(s) 633, 843, 1683
FokI GGATG 6 cut(s) 328, 2238, 2275, 2288, 2310, 2537
FriOI GRGCYC 1 cut(s) 2393
FspBI CTAG 3 cut(s) 14, 617, 2555
GlaI GCGC 1 cut(s) 638
GsuI CTGGAG 3 cut(s) 1118, 2240, 2548
HaeIII GGCC 3 cut(s) 2414, 2453, 2618
HapII CCGG 3 cut(s) 735, 1365, 2255
HgaI GACGC 2 cut(s) 430, 2918
HhaI GCGC 1 cut(s) 639
Hin1I GRCGYC 1 cut(s) 2910
Hin6I GCGC 1 cut(s) 637
HinP1I GCGC 1 cut(s) 637
HincII GTYRAC 2 cut(s) 370, 2908
HindII GTYRAC 2 cut(s) 370, 2908
HindIII AAGCTT 1 cut(s) 674
HpaII CCGG 3 cut(s) 735, 1365, 2255
HphI GGTGA 6 cut(s) 539, 2269, 2317, 2335, 2395, 2718
Hpy166II GTNNAC 6 cut(s) 370, 547, 2343, 2601, 2908, 2957
Hpy8I GTNNAC 6 cut(s) 370, 547, 2343, 2601, 2908, 2957
Hpy99I CGWCG 5 cut(s) 976, 1186, 1606, 1816, 2195
HpyAV CCTTC 9 cut(s) 404, 527, 2170, 2380, 2398, 2419, 2495, 2669, 2923
HpyCH4III ACNGT 2 cut(s) 389, 2656
HpyCH4IV ACGT 1 cut(s) 2591
HpyF10VI GCNNNNNNNGC 1 cut(s) 2298
HpyF3I CTNAG 9 cut(s) 166, 215, 690, 1565, 2435, 2487, 2819, 2832, 2839
HpySE526I ACGT 1 cut(s) 2591
Hsp92I GRCGYC 1 cut(s) 2910
HspAI GCGC 1 cut(s) 637
Kzo9I GATC 8 cut(s) 655, 1495, 1731, 1915, 1941, 2125, 2310, 2636
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 1 cut(s) 2558
LweI GCATC 8 cut(s) 2191, 2260, 2288, 2310, 2364, 2576, 2870, 2916
MaeI CTAG 3 cut(s) 14, 617, 2555
MaeII ACGT 1 cut(s) 2591
MaeIII GTNAC 3 cut(s) 233, 2323, 2519
MalI GATC 8 cut(s) 657, 1497, 1733, 1917, 1943, 2127, 2312, 2638
MboI GATC 8 cut(s) 655, 1495, 1731, 1915, 1941, 2125, 2310, 2636
MflI RGATCY 3 cut(s) 1731, 1941, 2310
MhlI GDGCHC 2 cut(s) 276, 2393
MlsI TGGCCA 1 cut(s) 2453
MluNI TGGCCA 1 cut(s) 2453
MmeI TCCRAC 1 cut(s) 369
Mox20I TGGCCA 1 cut(s) 2453
Mph1103I ATGCAT 2 cut(s) 6, 2303
MroXI GAANNNNTTC 3 cut(s) 36, 2154, 2339
MscI TGGCCA 1 cut(s) 2453
MslI CAYNNNNRTG 9 cut(s) 491, 632, 842, 1052, 1262, 1472, 1682, 2348, 2514
Msp20I TGGCCA 1 cut(s) 2453
MspA1I CMGCKG 2 cut(s) 2218, 2526
MspI CCGG 3 cut(s) 735, 1365, 2255
MspR9I CCNGG 6 cut(s) 63, 152, 313, 736, 1366, 2294
Mva1269I GAATGC 4 cut(s) 837, 1047, 1257, 1677
MvaI CCWGG 4 cut(s) 63, 152, 313, 2294
MwoI GCNNNNNNNGC 1 cut(s) 2298
NciI CCSGG 2 cut(s) 736, 1366
NdeI CATATG 3 cut(s) 633, 843, 1683
NdeII GATC 8 cut(s) 655, 1495, 1731, 1915, 1941, 2125, 2310, 2636
NlaIV GGNNCC 3 cut(s) 495, 2506, 2560
NmeAIII GCCGAG 1 cut(s) 2440
NmuCI GTSAC 3 cut(s) 233, 2323, 2519
NsiI ATGCAT 2 cut(s) 6, 2303
NspI RCATGY 3 cut(s) 382, 2471, 2691
NspV TTCGAA 1 cut(s) 2661
OliI CACNNNNGTG 1 cut(s) 491
PaeR7I CTCGAG 7 cut(s) 880, 1090, 1300, 1510, 1720, 1930, 2140
PciI ACATGT 2 cut(s) 2467, 2687
PciSI GCTCTTC 1 cut(s) 51
PcsI WCGNNNNNNNCGW 1 cut(s) 2196
PctI GAATGC 4 cut(s) 837, 1047, 1257, 1677
PdmI GAANNNNTTC 3 cut(s) 36, 2154, 2339
PfeI GAWTC 4 cut(s) 47, 321, 414, 613
PflMI CCANNNNNTGG 1 cut(s) 424
PfoI TCCNGGA 1 cut(s) 150
Ppu21I YACGTR 1 cut(s) 2592
PpuMI RGGWCCY 1 cut(s) 2504
PscI ACATGT 2 cut(s) 2467, 2687
PshBI ATTAAT 6 cut(s) 978, 1188, 1398, 1608, 1818, 2028
Psp5II RGGWCCY 1 cut(s) 2504
Psp6I CCWGG 4 cut(s) 61, 150, 311, 2292
PspGI CCWGG 4 cut(s) 61, 150, 311, 2292
PspN4I GGNNCC 3 cut(s) 495, 2506, 2560
PspPI GGNCC 7 cut(s) 1216, 1426, 1636, 1846, 2056, 2504, 2616
PspPPI RGGWCCY 1 cut(s) 2504
PspXI VCTCGAGB 7 cut(s) 880, 1090, 1300, 1510, 1720, 1930, 2140
PstNI CAGNNNCTG 2 cut(s) 265, 424
PsuI RGATCY 3 cut(s) 1731, 1941, 2310
PvuII CAGCTG 1 cut(s) 2526
RsaI GTAC 3 cut(s) 245, 1007, 2594
RsaNI GTAC 3 cut(s) 244, 1006, 2593
RseI CAYNNNNRTG 9 cut(s) 491, 632, 842, 1052, 1262, 1472, 1682, 2348, 2514
SapI GCTCTTC 1 cut(s) 51
Sau3AI GATC 8 cut(s) 655, 1495, 1731, 1915, 1941, 2125, 2310, 2636
Sau96I GGNCC 7 cut(s) 1216, 1426, 1636, 1846, 2056, 2504, 2616
ScrFI CCNGG 6 cut(s) 63, 152, 313, 736, 1366, 2294
SduI GDGCHC 2 cut(s) 276, 2393
SfaNI GCATC 8 cut(s) 2191, 2260, 2288, 2310, 2364, 2576, 2870, 2916
Sfr274I CTCGAG 7 cut(s) 880, 1090, 1300, 1510, 1720, 1930, 2140
SfuI TTCGAA 1 cut(s) 2661
SinI GGWCC 6 cut(s) 1216, 1426, 1636, 1846, 2056, 2504
SlaI CTCGAG 7 cut(s) 880, 1090, 1300, 1510, 1720, 1930, 2140
SmiMI CAYNNNNRTG 9 cut(s) 491, 632, 842, 1052, 1262, 1472, 1682, 2348, 2514
SsiI CCGC 5 cut(s) 2196, 2216, 2267, 2700, 2717
SspMI CTAG 3 cut(s) 14, 617, 2555
StyD4I CCNGG 6 cut(s) 61, 150, 311, 734, 1364, 2292
StyI CCWWGG 1 cut(s) 2554
TaaI ACNGT 2 cut(s) 389, 2656
TaiI ACGT 1 cut(s) 2594
TatI WGTACW 1 cut(s) 243
TauI GCSGC 3 cut(s) 2199, 2218, 2703
TfiI GAWTC 4 cut(s) 47, 321, 414, 613
TscAI CASTG 1 cut(s) 2294
TseFI GTSAC 3 cut(s) 233, 2323, 2519
Tsp45I GTSAC 3 cut(s) 233, 2323, 2519
TspGWI ACGGA 1 cut(s) 1424
TspRI CASTG 1 cut(s) 2294
Van91I CCANNNNNTGG 1 cut(s) 424
VpaK11BI GGWCC 6 cut(s) 1216, 1426, 1636, 1846, 2056, 2504
VspI ATTAAT 6 cut(s) 978, 1188, 1398, 1608, 1818, 2028
XapI RAATTY 2 cut(s) 437, 586
XceI RCATGY 3 cut(s) 382, 2471, 2691
XhoI CTCGAG 7 cut(s) 880, 1090, 1300, 1510, 1720, 1930, 2140
XmaJI CCTAGG 1 cut(s) 2554
XmnI GAANNNNTTC 3 cut(s) 36, 2154, 2339
XspI CTAG 3 cut(s) 14, 617, 2555
Zsp2I ATGCAT 2 cut(s) 6, 2303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.