MD07G1243000.v1.1

RESISTANCE protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000067
Physical Location & Seq
Reverse (-)
31322420 .. 31328307
5888 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1243000.v1.1.491

Sequence Viewer

Length: 2409 bp
ATGAAGAAAGACTGTGCAACCCGAATTCTGGAAGTTTGTGGTTTCCATCCTTATACTGGAATAAGAGTTCTAATCGATCGAGCTCTCATAACTGTCTCAGGGGAGGGGGAACTGGAGATGCATGATTCATTAGAGGAAATGGGTCGGGAAATCGTCCGCCAAGAATGTATCAAAGAGCCTGGGAGACGAAGTAGGTTGTGTAGTTATGAAGATGTTCATCACGTGCTATCTCAAAATACGGCTACAAAATCAGTTGAAAGCATAATCGTGGATTTCTCGTACTCAGACTGGGTATGCTTAAATGCTGAAGCTTTTGTTAGCATGAATCAACTAAGACTTATCAAGATCAGTCATAAGGACTCCATTTCTAAACATGATTACTGCAAACACCACTTGATTGGGCCCTTTAAGTTACTTAACTTGAGGTATCTCTCCTGGTTTGGATTCCCTCTCAAGTCTTTGCTATCCAACTTTCAATTCAAAAATCTTGTTGAACTTGACATGCAATTTAGTCTCATTGACCGACTTTGGGAAGGAACCCAGACGCTGGAAAAGTTGGAAGTCATCAATTTAAGTTATTGTCAATACCTTGAGGAAACCCCTGACTTCACAAATGTGCCAAATCTTGAGAGGCTAATTCTTCAAGGTTGTAGAAGCTTAGTTGAGGTTCACCAGTCTATTAGGACTCTTAGAAACCTTGTTTTATTGAATCTGAAAGGGTGCGAGAAACTTAAGATTCTACCCAGCAGCATTCGTATGAAATCTCTCAAAACCTTTAATCTTTCTGGCTGCTCCAGTCTTGAGATGTTTCCAGAGATTTTAGAAGGTATGGAGGAGTTAGAAGAGCTTGATTTATCCAAGTCAAAAATTAAAGAACTGTCCTCGTCAATTAATAATCTCACGGGGTTGAGTCATTTGAACCTAGAAAATTGCAAGGAACTTAAGAGTCTTCCAAGCAACATTCGTATCAAATCTCTCAAAACCTTTAATCTTTCTGGCTGCTGGAGTCTTGAGATGTTTCCAGAGATTTTACAAGTGATTGAGGGGTTAGAACAGCTTGATTTATCTAGGTCAAAAATTAAAGAACTGCCATCGTCAATTAATAACCTCACGGGGTTGAGTCGTTTGATCCTAGATGATTGCAAGGAACTTAAGTGTCTTCCAAGTAGCATTCAGATTTCAGAAGTGATTGAGGGGTTAGAAGTTCTTGATTTATCCGGGTCAAAAATTAAAGAATTGCCCCCATCAATTAATAATCTCAGTGGTTTGAGTTATTTAAGCCTAGATAATTGCGAGGAACTTAAGTGTCTTCCAAGCAGCATTCGTATGAAATCTCTCACAACCCTTAATCTTTCTGGCTGCTCGAGTCTAGAGATGTTTCCAGAGATTTCAGAAGTGATTGAGGGGTTAGAAGAGCTTGAGTTATCCGGGTCAAAAATTAAAGAACTACCCCCGTCAATTAATAACCTCACGGGGTTGAGTCATTTGCACCTAGATGATTGCAAGGAACTTAAGTGTCTTCCAAGCAGCATTCGTATGAAATCTCTCAAAGTGCTTAATCTTTCTGGTTGCTCGAGTCTAGAGATGTTTCCAGAGATTTCAGAAGTGATCGAGGGGTTAGAATTTCTTGATTTATCCGGGTCAAAAATTAAAGAATTGCCCTCGTCAATTAATAATCTCACTGGGTTGAGTTATTTAAGCTTGGATCATTGCGAGGAACTTAAGTGTCTTTCAAGCAGCATTCTTGTGAAATCTCTCAAAGCCGTTAATCTTTCTGGATGCTCGAGTCTAGAGATGTTTCCAGAGATTTCAGAAGTGATTGAGGGGTTAGAAGAGCTTGAGTTATCCGGGTCAAAAATTAAAGAACTACCCCTGTCAATTAATAACCTCACGGGGTTGAGTCATTTGCTCCTAGATGATTGCAAGGAACTTAAGTGTCTTCCAAGCAGCATTCGTATGAAATCTCTCAAAACTCTTAATCTTTCTGGTTGCTCGAGTCTAGAGATGTTTCCAGAGATTTCAGAAGTGATTGAGGGGTTAAAAGTTCTTTATTTTTCCGGGTCAAAAATTAAAGAACTGCCCTCGTCAATTAATAATCTCACGGGATTGAGTTATTTAAAGCTAGATGATTGCAAGGAACTTAAGGGTCTTCCAAGCAGCATTTGTCAGCTCAAGTCCCTGGTCTATCTCTCTCTTTCCGGTTGTACAAAGTTTGAGGTGTTTCCAAGCATTGAAGAAAATATGGAAGGATTAAGAGCGCTTTTCTTGGATGGAACATCTATCAAAGAGCTTTCCCCCTGGATTGAACGCCTTACGGGGCTTCAGTATTTAAATCTGAGAAACTGCAAAAGCCTTGTGCATCTTCCCGACACCCTCTGTAAATTGGCACACCTGATCACGGACACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

803

Amino Acids

89.84

Weight (kDa)

5.33

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 8 - 54 2e-10 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 254 - 334 1.4e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_EndoS PF23952 282 - 374 4.7e-07 Endo-beta-N-acetylglucosaminidase EndoS LRR domain
LRR_13 PF23286 324 - 393 4.3e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 398 - 467 3.9e-06 Leucine-rich repeat region
LRR_13 PF23286 444 - 513 4.5e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 449 - 527 5.7e-06 Leucine-rich repeat region
LRR_13 PF23286 514 - 597 3.7e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 515 - 583 6.7e-06 Leucine-rich repeat region
LRR_13 PF23286 584 - 653 3.1e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 647 - 754 2.7e-11 Leucine-rich repeat region
LRR_13 PF23286 654 - 737 5.7e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 547
AciI CCGC 1 cut(s) 157
AclWI GGATC 2 cut(s) 1123, 1713
AcsI RAATTY 2 cut(s) 24, 1622
AcuI CTGAAG 2 cut(s) 327, 2306
AcvI CACGTG 1 cut(s) 223
AfaI GTAC 2 cut(s) 281, 2206
AfeI AGCGCT 1 cut(s) 2259
AfiI CCNNNNNNNGG 5 cut(s) 28, 56, 529, 547, 2398
AflII CTTAAG 8 cut(s) 731, 941, 1151, 1301, 1511, 1721, 1931, 2141
AjnI CCWGG 4 cut(s) 178, 434, 2178, 2297
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AleI CACNNNNGTG 1 cut(s) 614
AloI GAACNNNNNNTCC 2 cut(s) 51, 83
Alw21I GWGCWC 1 cut(s) 85
Alw26I GTCTC 3 cut(s) 100, 178, 518
AlwI GGATC 2 cut(s) 1123, 1713
AlwNI CAGNNNCTG 1 cut(s) 547
Ama87I CYCGRG 4 cut(s) 1363, 1573, 1783, 1993
Aor51HI AGCGCT 1 cut(s) 2259
AoxI GGCC 1 cut(s) 401
ApaI GGGCCC 1 cut(s) 405
ApeKI GCWGC 9 cut(s) 747, 789, 999, 1317, 1359, 1527, 1737, 1947, 2157
ApoI RAATTY 2 cut(s) 24, 1622
AseI ATTAAT 7 cut(s) 891, 1101, 1251, 1461, 1671, 1881, 2091
Asp700I GAANNNNTTC 1 cut(s) 213
AspLEI GCGC 1 cut(s) 2260
AspS9I GGNCC 2 cut(s) 401, 402
AsuC2I CCSGG 5 cut(s) 1219, 1429, 1639, 1849, 2059
AsuHPI GGTGA 1 cut(s) 662
AvaI CYCGRG 4 cut(s) 1363, 1573, 1783, 1993
BaeGI GKGCMC 1 cut(s) 405
BaeI ACNNNNGTAYC 2 cut(s) 949, 982
BanII GRGCYC 2 cut(s) 85, 405
BbrPI CACGTG 1 cut(s) 223
BbsI GAAGAC 6 cut(s) 941, 1151, 1301, 1511, 1931, 2141
Bbv12I GWGCWC 1 cut(s) 85
BbvI GCAGC 9 cut(s) 759, 776, 986, 1329, 1346, 1539, 1749, 1959, 2169
BccI CCATC 4 cut(s) 54, 1099, 1252, 2264
BceAI ACGGC 2 cut(s) 255, 1748
BciT130I CCWGG 4 cut(s) 180, 436, 2180, 2299
BclI TGATCA 1 cut(s) 2394
BcnI CCSGG 5 cut(s) 1219, 1429, 1639, 1849, 2059
BcoDI GTCTC 3 cut(s) 100, 178, 518
BfoI RGCGCY 1 cut(s) 2261
BfrI CTTAAG 8 cut(s) 731, 941, 1151, 1301, 1511, 1721, 1931, 2141
BisI GCNGC 9 cut(s) 748, 790, 1000, 1318, 1360, 1528, 1738, 1948, 2158
BlsI GCNGC 9 cut(s) 749, 791, 1001, 1319, 1361, 1529, 1739, 1949, 2159
Bme1390I CCNGG 9 cut(s) 180, 436, 1219, 1429, 1639, 1849, 2059, 2180, 2299
BmeT110I CYCGRG 4 cut(s) 1363, 1573, 1783, 1993
BmgT120I GGNCC 2 cut(s) 401, 402
BmiI GGNNCC 2 cut(s) 403, 538
BmrFI CCNGG 9 cut(s) 180, 436, 1219, 1429, 1639, 1849, 2059, 2180, 2299
BmrI ACTGGG 2 cut(s) 298, 1692
BmsI GCATC 3 cut(s) 108, 1769, 2368
BmuI ACTGGG 2 cut(s) 298, 1692
BpiI GAAGAC 6 cut(s) 941, 1151, 1301, 1511, 1931, 2141
BpmI CTGGAG 3 cut(s) 134, 778, 1024
BpuEI CTTGAG 9 cut(s) 437, 442, 611, 647, 821, 1031, 1439, 1859, 2156
BpuMI CCSGG 5 cut(s) 1219, 1429, 1639, 1849, 2059
Bsa29I ATCGAT 1 cut(s) 75
BsaAI YACGTR 1 cut(s) 223
BsaBI GATNNNNATC 1 cut(s) 216
BsaJI CCNNGG 3 cut(s) 179, 2178, 2297
BsaWI WCCGGW 1 cut(s) 2198
BsaXI ACNNNNNCTCC 2 cut(s) 175, 205
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 56, 529, 547, 2398
Bse1I ACTGG 6 cut(s) 61, 117, 293, 673, 795, 1687
Bse3DI GCAATG 1 cut(s) 1708
Bse8I GATNNNNATC 1 cut(s) 216
BseBI CCWGG 4 cut(s) 180, 436, 2180, 2299
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 3 cut(s) 179, 2178, 2297
BseGI GGATG 3 cut(s) 46, 1784, 2275
BseJI GATNNNNATC 1 cut(s) 216
BseLI CCNNNNNNNGG 5 cut(s) 28, 56, 529, 547, 2398
BseMI GCAATG 1 cut(s) 1708
BseMII CTCAG 4 cut(s) 111, 297, 1273, 2327
BseNI ACTGG 6 cut(s) 61, 117, 293, 673, 795, 1687
BseRI GAGGAG 1 cut(s) 848
BseSI GKGCMC 1 cut(s) 405
BseXI GCAGC 9 cut(s) 759, 776, 986, 1329, 1346, 1539, 1749, 1959, 2169
BseYI CCCAGC 1 cut(s) 743
Bsh1285I CGRYCG 1 cut(s) 79
BshFI GGCC 1 cut(s) 403
BshVI ATCGAT 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 79
BsiHKAI GWGCWC 1 cut(s) 85
BsiHKCI CYCGRG 4 cut(s) 1363, 1573, 1783, 1993
BsiSI CCGG 6 cut(s) 1218, 1428, 1638, 1848, 2058, 2199
BslFI GGGAC 1 cut(s) 2161
BslI CCNNNNNNNGG 5 cut(s) 28, 56, 529, 547, 2398
BsmAI GTCTC 3 cut(s) 100, 178, 518
BsmBI CGTCTC 1 cut(s) 178
BsmFI GGGAC 1 cut(s) 2161
BsmI GAATGC 6 cut(s) 750, 1170, 1320, 1530, 1740, 1950
BsnI GGCC 1 cut(s) 403
BsoBI CYCGRG 4 cut(s) 1363, 1573, 1783, 1993
Bsp120I GGGCCC 1 cut(s) 401
Bsp1286I GDGCHC 2 cut(s) 85, 405
Bsp1407I TGTACA 1 cut(s) 2204
Bsp143I GATC 6 cut(s) 76, 345, 1128, 1608, 1705, 2394
BspACI CCGC 1 cut(s) 157
BspANI GGCC 1 cut(s) 403
BspCNI CTCAG 4 cut(s) 110, 296, 1272, 2328
BspDI ATCGAT 1 cut(s) 75
BspLI GGNNCC 2 cut(s) 403, 538
BspPI GGATC 2 cut(s) 1123, 1713
BspQI GCTCTTC 3 cut(s) 837, 1407, 1827
BspTI CTTAAG 8 cut(s) 731, 941, 1151, 1301, 1511, 1721, 1931, 2141
BsrDI GCAATG 1 cut(s) 1708
BsrGI TGTACA 1 cut(s) 2204
BsrI ACTGG 6 cut(s) 61, 117, 293, 673, 795, 1687
BssECI CCNNGG 3 cut(s) 179, 2178, 2297
BssMI GATC 6 cut(s) 76, 345, 1128, 1608, 1705, 2394
Bst2UI CCWGG 4 cut(s) 180, 436, 2180, 2299
Bst4CI ACNGT 3 cut(s) 14, 94, 879
Bst6I CTCTTC 3 cut(s) 837, 1407, 1827
BstAFI CTTAAG 8 cut(s) 731, 941, 1151, 1301, 1511, 1721, 1931, 2141
BstAUI TGTACA 1 cut(s) 2204
BstBAI YACGTR 1 cut(s) 223
BstDEI CTNAG 7 cut(s) 97, 283, 332, 658, 689, 1259, 2336
BstF5I GGATG 3 cut(s) 46, 1784, 2275
BstH2I RGCGCY 1 cut(s) 2261
BstHHI GCGC 1 cut(s) 2260
BstKTI GATC 6 cut(s) 79, 348, 1131, 1611, 1708, 2397
BstMAI GTCTC 3 cut(s) 100, 178, 518
BstMBI GATC 6 cut(s) 76, 345, 1128, 1608, 1705, 2394
BstMCI CGRYCG 1 cut(s) 79
BstNI CCWGG 4 cut(s) 180, 436, 2180, 2299
BstNSI RCATGY 1 cut(s) 505
BstSCI CCNGG 9 cut(s) 178, 434, 1217, 1427, 1637, 1847, 2057, 2178, 2297
BstSLI GKGCMC 1 cut(s) 405
BstV1I GCAGC 9 cut(s) 759, 776, 986, 1329, 1346, 1539, 1749, 1959, 2169
BstV2I GAAGAC 6 cut(s) 941, 1151, 1301, 1511, 1931, 2141
BstXI CCANNNNNNTGG 1 cut(s) 398
Bsu15I ATCGAT 1 cut(s) 75
BsuRI GGCC 1 cut(s) 403
BsuTUI ATCGAT 1 cut(s) 75
BtsCI GGATG 3 cut(s) 46, 1784, 2275
BtsIMutI CAGTG 2 cut(s) 1267, 1680
CaiI CAGNNNCTG 1 cut(s) 547
CfoI GCGC 1 cut(s) 2260
Cfr13I GGNCC 2 cut(s) 401, 402
ClaI ATCGAT 1 cut(s) 75
CseI GACGC 1 cut(s) 553
Csp6I GTAC 2 cut(s) 280, 2205
CviAII CATG 4 cut(s) 122, 322, 374, 502
CviQI GTAC 2 cut(s) 280, 2205
DdeI CTNAG 7 cut(s) 97, 283, 332, 658, 689, 1259, 2336
DpnI GATC 6 cut(s) 78, 347, 1130, 1610, 1707, 2396
DpnII GATC 6 cut(s) 76, 345, 1128, 1608, 1705, 2394
DraI TTTAAA 2 cut(s) 2118, 2331
Eam1104I CTCTTC 3 cut(s) 837, 1407, 1827
EarI CTCTTC 3 cut(s) 837, 1407, 1827
EciI GGCGGA 1 cut(s) 146
Ecl136II GAGCTC 1 cut(s) 83
Eco24I GRGCYC 2 cut(s) 85, 405
Eco47III AGCGCT 1 cut(s) 2259
Eco53kI GAGCTC 1 cut(s) 83
Eco57I CTGAAG 2 cut(s) 327, 2306
Eco72I CACGTG 1 cut(s) 223
Eco88I CYCGRG 4 cut(s) 1363, 1573, 1783, 1993
EcoICRI GAGCTC 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 402
EcoRI GAATTC 1 cut(s) 24
EcoRII CCWGG 4 cut(s) 178, 434, 2178, 2297
EcoT22I ATGCAT 1 cut(s) 123
EcoT38I GRGCYC 2 cut(s) 85, 405
Esp3I CGTCTC 1 cut(s) 178
FaeI CATG 4 cut(s) 125, 325, 377, 505
FaqI GGGAC 1 cut(s) 2161
FatI CATG 4 cut(s) 121, 321, 373, 501
FbaI TGATCA 1 cut(s) 2394
Fnu4HI GCNGC 9 cut(s) 748, 790, 1000, 1318, 1360, 1528, 1738, 1948, 2158
FokI GGATG 3 cut(s) 33, 1791, 2282
FriOI GRGCYC 2 cut(s) 85, 405
Fsp4HI GCNGC 9 cut(s) 748, 790, 1000, 1318, 1360, 1528, 1738, 1948, 2158
GlaI GCGC 1 cut(s) 2259
GluI GCNGC 9 cut(s) 748, 790, 1000, 1318, 1360, 1528, 1738, 1948, 2158
GsaI CCCAGC 1 cut(s) 747
GsuI CTGGAG 3 cut(s) 134, 778, 1024
HaeII RGCGCY 1 cut(s) 2261
HaeIII GGCC 1 cut(s) 403
HapII CCGG 6 cut(s) 1218, 1428, 1638, 1848, 2058, 2199
HgaI GACGC 1 cut(s) 553
HhaI GCGC 1 cut(s) 2260
Hin1II CATG 4 cut(s) 125, 325, 377, 505
Hin6I GCGC 1 cut(s) 2258
HinP1I GCGC 1 cut(s) 2258
HindIII AAGCTT 3 cut(s) 309, 655, 1699
HpaII CCGG 6 cut(s) 1218, 1428, 1638, 1848, 2058, 2199
HphI GGTGA 1 cut(s) 662
Hpy166II GTNNAC 1 cut(s) 670
Hpy188I TCNGA 9 cut(s) 286, 714, 1176, 1183, 1393, 1603, 1813, 2023, 2337
Hpy8I GTNNAC 1 cut(s) 670
HpyAV CCTTC 3 cut(s) 527, 818, 2240
HpyCH4III ACNGT 3 cut(s) 14, 94, 879
HpyCH4IV ACGT 1 cut(s) 222
HpyF3I CTNAG 7 cut(s) 97, 283, 332, 658, 689, 1259, 2336
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 4 cut(s) 125, 325, 377, 505
HspAI GCGC 1 cut(s) 2258
Ksp22I TGATCA 1 cut(s) 2394
Kzo9I GATC 6 cut(s) 76, 345, 1128, 1608, 1705, 2394
LguI GCTCTTC 3 cut(s) 837, 1407, 1827
LmnI GCTCC 2 cut(s) 797, 1914
Lsp1109I GCAGC 9 cut(s) 759, 776, 986, 1329, 1346, 1539, 1749, 1959, 2169
LweI GCATC 3 cut(s) 108, 1769, 2368
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 411
MalI GATC 6 cut(s) 78, 347, 1130, 1610, 1707, 2396
MboI GATC 6 cut(s) 76, 345, 1128, 1608, 1705, 2394
MhlI GDGCHC 2 cut(s) 85, 405
MmeI TCCRAC 2 cut(s) 492, 537
Mph1103I ATGCAT 1 cut(s) 123
MroXI GAANNNNTTC 1 cut(s) 213
MslI CAYNNNNRTG 8 cut(s) 266, 614, 755, 1325, 1494, 1535, 1745, 1955
MspCI CTTAAG 8 cut(s) 731, 941, 1151, 1301, 1511, 1721, 1931, 2141
MspI CCGG 6 cut(s) 1218, 1428, 1638, 1848, 2058, 2199
MspR9I CCNGG 9 cut(s) 180, 436, 1219, 1429, 1639, 1849, 2059, 2180, 2299
Mva1269I GAATGC 6 cut(s) 750, 1170, 1320, 1530, 1740, 1950
MvaI CCWGG 4 cut(s) 180, 436, 2180, 2299
NciI CCSGG 5 cut(s) 1219, 1429, 1639, 1849, 2059
NdeII GATC 6 cut(s) 76, 345, 1128, 1608, 1705, 2394
NlaIII CATG 4 cut(s) 125, 325, 377, 505
NlaIV GGNNCC 2 cut(s) 403, 538
NsiI ATGCAT 1 cut(s) 123
NspI RCATGY 1 cut(s) 505
OliI CACNNNNGTG 1 cut(s) 614
PaeR7I CTCGAG 4 cut(s) 1363, 1573, 1783, 1993
PciSI GCTCTTC 3 cut(s) 837, 1407, 1827
PctI GAATGC 6 cut(s) 750, 1170, 1320, 1530, 1740, 1950
PdmI GAANNNNTTC 1 cut(s) 213
PfeI GAWTC 5 cut(s) 125, 325, 444, 709, 736
PflMI CCANNNNNTGG 1 cut(s) 547
PkrI GCNGC 9 cut(s) 749, 791, 1001, 1319, 1361, 1529, 1739, 1949, 2159
Ple19I CGATCG 1 cut(s) 79
PmaCI CACGTG 1 cut(s) 223
PmlI CACGTG 1 cut(s) 223
Ppu21I YACGTR 1 cut(s) 223
PshBI ATTAAT 7 cut(s) 891, 1101, 1251, 1461, 1671, 1881, 2091
Psp124BI GAGCTC 1 cut(s) 85
Psp6I CCWGG 4 cut(s) 178, 434, 2178, 2297
PspCI CACGTG 1 cut(s) 223
PspFI CCCAGC 1 cut(s) 743
PspGI CCWGG 4 cut(s) 178, 434, 2178, 2297
PspN4I GGNNCC 2 cut(s) 403, 538
PspOMI GGGCCC 1 cut(s) 401
PspPI GGNCC 2 cut(s) 401, 402
PspXI VCTCGAGB 4 cut(s) 1363, 1573, 1783, 1993
PstNI CAGNNNCTG 1 cut(s) 547
PvuI CGATCG 1 cut(s) 79
RsaI GTAC 2 cut(s) 281, 2206
RsaNI GTAC 2 cut(s) 280, 2205
RseI CAYNNNNRTG 8 cut(s) 266, 614, 755, 1325, 1494, 1535, 1745, 1955
SacI GAGCTC 1 cut(s) 85
SapI GCTCTTC 3 cut(s) 837, 1407, 1827
SatI GCNGC 9 cut(s) 748, 790, 1000, 1318, 1360, 1528, 1738, 1948, 2158
Sau3AI GATC 6 cut(s) 76, 345, 1128, 1608, 1705, 2394
Sau96I GGNCC 2 cut(s) 401, 402
ScrFI CCNGG 9 cut(s) 180, 436, 1219, 1429, 1639, 1849, 2059, 2180, 2299
SduI GDGCHC 2 cut(s) 85, 405
SfaNI GCATC 3 cut(s) 108, 1769, 2368
Sfr274I CTCGAG 4 cut(s) 1363, 1573, 1783, 1993
SlaI CTCGAG 4 cut(s) 1363, 1573, 1783, 1993
SmiI ATTTAAAT 1 cut(s) 2331
SmiMI CAYNNNNRTG 8 cut(s) 266, 614, 755, 1325, 1494, 1535, 1745, 1955
SsiI CCGC 1 cut(s) 157
SstI GAGCTC 1 cut(s) 85
StyD4I CCNGG 9 cut(s) 178, 434, 1217, 1427, 1637, 1847, 2057, 2178, 2297
SwaI ATTTAAAT 1 cut(s) 2331
TaaI ACNGT 3 cut(s) 14, 94, 879
TaiI ACGT 1 cut(s) 225
TaqI TCGA 7 cut(s) 75, 79, 1364, 1574, 1611, 1784, 1994
TaqII GACCGA 1 cut(s) 537
TatI WGTACW 1 cut(s) 2204
TfiI GAWTC 5 cut(s) 125, 325, 444, 709, 736
TscAI CASTG 2 cut(s) 1267, 1687
TseI GCWGC 9 cut(s) 747, 789, 999, 1317, 1359, 1527, 1737, 1947, 2157
TspDTI ATGAA 9 cut(s) 17, 117, 206, 222, 338, 773, 1343, 1553, 1973
TspRI CASTG 2 cut(s) 1267, 1687
Van91I CCANNNNNTGG 1 cut(s) 547
Vha464I CTTAAG 8 cut(s) 731, 941, 1151, 1301, 1511, 1721, 1931, 2141
VspI ATTAAT 7 cut(s) 891, 1101, 1251, 1461, 1671, 1881, 2091
XapI RAATTY 2 cut(s) 24, 1622
XbaI TCTAGA 4 cut(s) 1369, 1579, 1789, 1999
XceI RCATGY 1 cut(s) 505
XcmI CCANNNNNNNNNTGG 1 cut(s) 53
XhoI CTCGAG 4 cut(s) 1363, 1573, 1783, 1993
XmnI GAANNNNTTC 1 cut(s) 213
Zsp2I ATGCAT 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.