FvH4_7g28550

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000067
Physical Location & Seq
Forward (+)
21151445 .. 21158170
6726 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g28550.t3

Sequence Viewer

Length: 4002 bp
ATGGCCGCTTCTTCTTCTTCTTCTTCTTCCTCTGTCGGCCGTTGGAACTACGATGTCTTCATCAGCTTCAAAGCCGACGACACCCGCAAGATCTTCGTCGGCCATCTCCACAAGGCTCTGCATGAGAAAGCAATCAACACATTCCTCGACTCCGACGACCCTTCAGAGGTTATGGAAGCCATCGCCGACTCGAGGCTGTCCGTTGTTGTGTTCTCCAAACACTATGCTTCTTCCAGGTGGTGCATGAAGGAGCTGGTGAGGATCATGGAGTGCATGAAAACCATGAAGCAGATTGTGGTGCCTGTTTTCTACGAACTTGATCGTTGTGATATTAAGTCGAGTTTTGAGGAAGGGTTTGGTAAGCATGAAGTAGAAGAGGTGCGGAGATTGAAGTCTCTTCTGATTCAAGCCGCCAGTTTATCTTGCTGGGATTCCCGGAATTATCGGGATGATGCCAAGTTTATTGAGAAAATCGTCGACGATATTTCTAAGAGATTGATACGGGTGAGACCTGGAAAAGAGAATTGTTTGGTTGGAATGGATAGACACATAGCAGAAGTAGATAGTATGTTGTGTCTTGGGGTGGATGATGTTCGTGGTATTGGGATATACGGAATGCCTGGTATAGGTAAAACAACCATTGCTCGAGCTGTTTATGATGAAATCACTTGTCAGTTTGAACACTACTGCTTTCTTGAAAATGTCAAAGATGGTTTCAAGAATAAAGGTGCAGAACGTCTGCAGGAAGAACTTCTATGTAGAATGTTGAAGAAAAAGGTGTGTAACTTAGGTGATTTGAATACTGGCTTTAAGATGGTTATGGAAAGGCTGAATAAGAAAAAAGTTTTGCTTGTTCTTGATGATGTGGAAACTTTTGCCCAGATTGAAGCCTTACTTGGAAAGCAATGTTCATTTGGTGGTGGAAGTAGAATCATTATAACAACTAGAGATATACAATCACTAAGTGGAGTGCAGGAGAGATATAGTCCCAAATTTTTAAGTGATGATGAAGCTCTTGAACTTTTTATGCAGTATGCATTCAGAACACACCAACCCACAAGAGAGTACGATTCTCTCTCAAGGCGTGCCATAGGATATGCTCGAGGTCTGCCTCTAGCACTTAAAGTTTTGGGAGCTTTTCTTGATTGCAAAAGTGCATGTGAGTGGGAAGATGAGCTAGAAAAAATAAAGAAAATCCCACACATGGAAATTCAGGGTGTGCTTAGAACAAGCTTGAATGGACTAGAGCCTTTACAGAAGGACATATTTCTAGATATTGCATGTTTCTTCAGAGGAATGGACAAAGGATATGTGACAAAAGTTCTGGAAAGTTGTGGCTTTTACCCCCCTAGTGGATTACGAGTACTACTTGATAGAGCTCTCATTACGATCTCAGATGACAATAGGCTCGAAATGCATGATCTACTACAAGAAATAGGCTGGGAAATTGTTCGACAACAATCAGTTAAAGATCCTGGGAAGCGCAGTAGGTTGTGGGTTTATGAAGATGTTGATCATGTCTTCACTCAAAATACGGCTACAGATGCAGTTGAGGGCATAATGCTCGACTTCTTAAGGTCAAAACAGGTATACTTAACTTCTGAAGCTTTTGTTAAAATGACGAGGCTGAGACTGCTAAGGATTATCAGTGATAACCATTCACGTGAGGATTTATGTCATCCAAGTGATGACTGTCAACAACAACTGGTTGGGGACTTCAAGTTTCTTTCTAATGAGTTGAGGGTTCTCCACTGGCATGGATTCCCCTTAAAGTCTTTGCCATCCAATTTTCACCCGAAGAATCTTGTTGAACTTGACATGCGTTCTAGTCACATTGAACAACTGTGGGAAGGAATTAAGCCTTTGAAAAAGTTGACAATTATTAATTTAAGTCACTCTCCATACCTTAAGAAAATCCCTGACTTCACTGAGGCGACGAATATGAAGAAACTGGTTCTTGATGGTTGTTTAAGTTTATTGGAGATTCATCCATCCATTTCAGCTCTTAAAAGCCTTGTTGTCATGAGTCTAAAAGGGTGCAAAGCAGTTAAGAGTCTTCCAGGGAGCATTTATATGAAGTCTCTTAAAATCCTTGATCTTTCTGGCTGCTCAAATCTTGAGATGTTTCCAGAGATCTCAGGAATTATGGAGGACCTATCGGAGCTTTATTTGAATGACACTGCAATTGAAGAATTACCGTCATCGATTGAACAGCTTCAGGGGCTTGTGTTATTGAATATGGCAAACTGCAGAAGCCTTGTTTGTCTTCCAGACAATATCTGTAATTTGGCTTACCTTACGAATCTCACTCTCTCTGGGTGCTCAAAGCTTTATCGTTTGCCTGAGAACTTGGGGAACCTTGAATCCTTGACAGTCCTTGAAGTAGAAAGAAGTGGTATAAAAGAACTCCCTTTTTCGATCATACGTGTGAATAAGCTTAAAAGATTATCATGTGATGGATGTAAAGAAATGATGGCCCCCCTTTCATCATGGTCATCACCAATCAAAGGATTTTATACTTACTCGGGTATTCTACATCTTGATTTAAGTGACTGCAATCTGTTTGTATTATCAGATGGTATTGCTCATTTGTCCTCATTAAGAACATTAGAGCTGCGCAGAAACAACTTGGAGAGCTTACCTGCAGTAATGAATCGACTTCATCGTTTATCACATCTTGAATTAGAAGCGTGCAAGAGACTGAAATCAATACCTGAGCTTTCCTCAAGTATTACTTACATAGATGCTCATGATTGCACAGCTTTGGAAACAGTTTCGACACCAAAGCCTCGGTCTTCTACAAATCTTTGCCTCACATTTTCAAATTGCCTCCAGCTGATACATACTAATCTATTTAGGGATATTGTGGAAACTCATTCTCATTATCAGGGTAGTTATCTGCGACCTCTTTCCTTTAGTATGTCACTTCCTGGAAGTGAAATTCCTCATTGGTTCAATCATCAGTGTAAGGGATTCTCAGTGAATGTGCAGCTACCACAGAATTGGTTTGACGATAAGTTCTTGGGATTTGCTTTATGTGTTGTTAGTAATTTCAAGGGTGCTCATAATGATGCATCCAATCTCTCTGCTATTTGTTATTGTACCTTTAAAGGAAATCATGGTGAGTACAAGTTCAATTTTCAATTGCTTGATTGGGGTTTCAGAACAAACAGATTCCTCCAGTCGGATCACATGTTCCTGGGGTATGTGCCATGGTCTCAATATCGCTGCAGTAATGAAGGGAAGCCGGTCAGTGAACGCTACTACACTGAAGCCACGTTTGAGATTGTGGTAGAAAATGGAGTCTATACACATGAGAATAACAGGACAGTAATACGCCGGCATTGCATCACAAGTTGCGGAGTGCGTTTGTTTCATGGTGATTACATGGAAGTGCAAAATCTAAGCATCGCTCAGCCTAATTCTCGTCATGATAGCTGTGAAATTCTATTGGACACTGCAAGGAAGAGAAAGAGAGATATAGCAGGGGTGACATTGCAGTGGGAGTGGCCTCCCACAGAAGAGGTGTTCACTGAGCAAGAGCAAGAAGGATCCCCACCAAGGGAAGGGTCCCTGGCTGGGGAAGGGCCCTCAGTGGGCAAGAGGTCCTCGGTGGGGGAAGGGGTTCTTTCTATCCTTTCTTCAGTAGCAACAGTAGAAAGCGGTGCCAATGAACGTGATGGTGCAACTATCCCTAATAATACTAACAGAAGAAGGCGTACAAATTTGCGAAACTCAGCGACGTGGATCCGGGATACAAGTCAGCTTATTGATGATGGCCATGCTTGGAGAAAGTATGGCCAAAAGGAGATACTTGGATCCAGACATCCCAGAAACTATTACAGATGCACTTATCGATTGGATCAAGGCTGTATGGCCACTAAGCACGTGCAACGCGTTAGTGATGAGCCAGTATTATACAGAACTACTTACAGAGGAAATCACACATGCAGACCCGTCTCCAGCATACAGGACGTGGAAGAGTTCTTTTTGGCATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1334

Amino Acids

151.67

Weight (kDa)

6.81

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 172 5.2e-36 TIR domain
TIR_2 PF13676 19 - 109 1.1e-11 TIR domain
NB-ARC PF00931 188 - 349 2.3e-18 NB-ARC domain
WHD_ROQ1 PF23282 419 - 488 7.3e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 601 - 620 5.9e-07 Leucine Rich Repeat
LRR_14 PF23598 662 - 772 1.1e-08 Leucine-rich repeat region
LRR_13 PF23286 692 - 777 7e-13 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 759 - 824 2e-09 Leucine-rich repeat region
LRR_14 PF23598 846 - 907 5.4e-06 Leucine-rich repeat region
LRR_8 PF13855 846 - 898 3.8e-06 Leucine rich repeat
C-JID PF20160 976 - 1127 9.1e-21 C-JID domain
WRKY PF03106 1258 - 1317 1.2e-23 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 938
Acc16I TGCGCA 1 cut(s) 2618
Acc36I ACCTGC 1 cut(s) 2650
AccB1I GGYRCC 2 cut(s) 298, 3665
AccI GTMKAC 2 cut(s) 477, 1590
AccII CGCG 1 cut(s) 3897
AciI CCGC 6 cut(s) 6, 85, 382, 411, 3360, 3663
AcoI YGGCCR 6 cut(s) 3, 37, 100, 3778, 3799, 3876
AcsI RAATTY 5 cut(s) 992, 1209, 2940, 3444, 3724
AcuI CTGAAG 6 cut(s) 147, 1273, 1623, 2201, 3291, 3627
AcvI CACGTG 2 cut(s) 1664, 3889
AdeI CACNNNGTG 1 cut(s) 965
AfaI GTAC 5 cut(s) 1067, 1365, 3103, 3128, 3721
AflII CTTAAG 2 cut(s) 1573, 1907
AflIII ACRYGT 3 cut(s) 2425, 3192, 3895
AjiI CACGTC 2 cut(s) 3744, 3976
AjnI CCWGG 8 cut(s) 233, 511, 619, 1474, 2059, 2929, 3198, 3573
AjuI GAANNNNNNNTTGG 2 cut(s) 879, 911
AloI GAACNNNNNNTCC 4 cut(s) 2348, 2380, 3179, 3211
Alw21I GWGCWC 3 cut(s) 1381, 2324, 3064
Alw26I GTCTC 7 cut(s) 399, 502, 1624, 2085, 2692, 3222, 3964
Ama87I CYCGRG 4 cut(s) 190, 645, 1101, 2524
AoxI GGCC 9 cut(s) 3, 37, 100, 2475, 3509, 3587, 3778, 3799, 3876
ApaI GGGCCC 1 cut(s) 3591
ApeKI GCWGC 4 cut(s) 2106, 2614, 2989, 3228
ApoI RAATTY 5 cut(s) 992, 1209, 2940, 3444, 3724
ArsI GACNNNNNNTTYG 2 cut(s) 2777, 2809
AseI ATTAAT 1 cut(s) 1884
Asp700I GAANNNNTTC 4 cut(s) 750, 1449, 2214, 3624
AspLEI GCGC 2 cut(s) 1485, 2619
AspS9I GGNCC 6 cut(s) 2152, 2476, 3570, 3587, 3588, 3606
AsuC2I CCSGG 2 cut(s) 436, 3752
AsuHPI GGTGA 8 cut(s) 268, 517, 803, 1784, 2490, 3134, 3392, 3502
AvaI CYCGRG 4 cut(s) 190, 645, 1101, 2524
AvaII GGWCC 3 cut(s) 2152, 3570, 3606
BaeGI GKGCMC 1 cut(s) 3591
BalI TGGCCA 3 cut(s) 3780, 3801, 3878
BamHI GGATCC 3 cut(s) 3551, 3747, 3818
BanI GGYRCC 2 cut(s) 298, 3665
BanII GRGCYC 2 cut(s) 1381, 3591
BarI GAAGNNNNNNTAC 2 cut(s) 3703, 3735
BbrPI CACGTG 2 cut(s) 1664, 3889
BbsI GAAGAC 5 cut(s) 49, 1513, 2048, 2258, 2787
Bbv12I GWGCWC 3 cut(s) 1381, 2324, 3064
BbvI GCAGC 4 cut(s) 2093, 2601, 3001, 3215
BceAI ACGGC 2 cut(s) 24, 1551
BcgI CGANNNNNNTGC 4 cut(s) 76, 110, 1546, 1580
BciT130I CCWGG 8 cut(s) 235, 513, 621, 1476, 2061, 2931, 3200, 3575
BciVI GTATCC 1 cut(s) 3748
BclI TGATCA 1 cut(s) 1513
BcnI CCSGG 2 cut(s) 436, 3752
BcoDI GTCTC 7 cut(s) 399, 502, 1624, 2085, 2692, 3222, 3964
BfaI CTAG 7 cut(s) 945, 1115, 1178, 1244, 1271, 1350, 1827
BfmI CTRYAG 5 cut(s) 740, 1539, 2248, 2643, 3229
BfrI CTTAAG 2 cut(s) 1573, 1907
BfuAI ACCTGC 1 cut(s) 2650
BfuI GTATCC 1 cut(s) 3748
BglII AGATCT 2 cut(s) 90, 2133
BisI GCNGC 6 cut(s) 6, 411, 2107, 2615, 2990, 3229
BlpI GCTNAGC 1 cut(s) 3414
BlsI GCNGC 6 cut(s) 7, 412, 2108, 2616, 2991, 3230
BmcAI AGTACT 1 cut(s) 1365
Bme18I GGWCC 3 cut(s) 2152, 3570, 3606
BmeT110I CYCGRG 4 cut(s) 190, 645, 1101, 2524
BmgBI CACGTC 2 cut(s) 3744, 3976
BmgT120I GGNCC 6 cut(s) 2152, 2476, 3570, 3587, 3588, 3606
BmsI GCATC 8 cut(s) 442, 1534, 2734, 3061, 3083, 3357, 3417, 3836
BpiI GAAGAC 5 cut(s) 49, 1513, 2048, 2258, 2787
BplI GAGNNNNNCTC 2 cut(s) 2708, 2740
BpmI CTGGAG 3 cut(s) 2816, 3164, 3946
Bpu10I CCTNAGC 2 cut(s) 1637, 2715
Bpu1102I GCTNAGC 1 cut(s) 3414
BpuEI CTTGAG 3 cut(s) 1063, 2138, 2710
BpuMI CCSGG 2 cut(s) 436, 3752
Bsa29I ATCGAT 2 cut(s) 2204, 3856
BsaAI YACGTR 3 cut(s) 1664, 2426, 3889
BsaBI GATNNNNATC 1 cut(s) 1512
BsaI GGTCTC 2 cut(s) 502, 3222
BsaJI CCNNGG 8 cut(s) 1475, 2060, 2789, 3199, 3212, 3560, 3573, 3609
Bse118I RCCGGY 2 cut(s) 3247, 3339
Bse1I ACTGG 7 cut(s) 414, 808, 1710, 1757, 1956, 3181, 3911
Bse3DI GCAATG 4 cut(s) 639, 911, 3343, 3494
Bse8I GATNNNNATC 1 cut(s) 1512
BseBI CCWGG 8 cut(s) 235, 513, 621, 1476, 2061, 2931, 3200, 3575
BseCI ATCGAT 2 cut(s) 2204, 3856
BseDI CCNNGG 8 cut(s) 1475, 2060, 2789, 3199, 3212, 3560, 3573, 3609
BseGI GGATG 9 cut(s) 454, 592, 1678, 1781, 1987, 1991, 2465, 3074, 3826
BseJI GATNNNNATC 1 cut(s) 1512
BseMI GCAATG 4 cut(s) 639, 911, 3343, 3494
BseNI ACTGG 7 cut(s) 414, 808, 1710, 1757, 1956, 3181, 3911
BseSI GKGCMC 1 cut(s) 3591
BseX3I CGGCCG 1 cut(s) 37
BseXI GCAGC 4 cut(s) 2093, 2601, 3001, 3215
BseYI CCCAGC 3 cut(s) 426, 1440, 3578
BsgI GTGCAG 3 cut(s) 750, 992, 3008
Bsh1236I CGCG 1 cut(s) 3897
Bsh1285I CGRYCG 1 cut(s) 40
BshFI GGCC 9 cut(s) 5, 39, 102, 2477, 3511, 3589, 3780, 3801, 3878
BshNI GGYRCC 2 cut(s) 298, 3665
BshVI ATCGAT 2 cut(s) 2204, 3856
BsiEI CGRYCG 1 cut(s) 40
BsiHKAI GWGCWC 3 cut(s) 1381, 2324, 3064
BsiHKCI CYCGRG 4 cut(s) 190, 645, 1101, 2524
BsiSI CCGG 4 cut(s) 436, 3248, 3340, 3751
BslFI GGGAC 3 cut(s) 972, 1727, 3556
BsmAI GTCTC 7 cut(s) 399, 502, 1624, 2085, 2692, 3222, 3964
BsmBI CGTCTC 1 cut(s) 3964
BsmFI GGGAC 3 cut(s) 972, 1727, 3556
BsmI GAATGC 2 cut(s) 621, 1037
BsnI GGCC 9 cut(s) 5, 39, 102, 2477, 3511, 3589, 3780, 3801, 3878
Bso31I GGTCTC 2 cut(s) 502, 3222
BsoBI CYCGRG 4 cut(s) 190, 645, 1101, 2524
Bsp120I GGGCCC 1 cut(s) 3587
Bsp1286I GDGCHC 4 cut(s) 1381, 2324, 3064, 3591
Bsp1720I GCTNAGC 1 cut(s) 3414
Bsp19I CCATGG 1 cut(s) 3212
BspACI CCGC 6 cut(s) 6, 85, 382, 411, 3360, 3663
BspANI GGCC 9 cut(s) 5, 39, 102, 2477, 3511, 3589, 3780, 3801, 3878
BspDI ATCGAT 2 cut(s) 2204, 3856
BspFNI CGCG 1 cut(s) 3897
BspHI TCATGA 3 cut(s) 2022, 2749, 3430
BspMAI CTGCAG 4 cut(s) 744, 2252, 2647, 3233
BspMI ACCTGC 1 cut(s) 2650
BspT107I GGYRCC 2 cut(s) 298, 3665
BspTI CTTAAG 2 cut(s) 1573, 1907
BspTNI GGTCTC 2 cut(s) 502, 3222
BsrDI GCAATG 4 cut(s) 639, 911, 3343, 3494
BsrFI RCCGGY 2 cut(s) 3247, 3339
BsrI ACTGG 7 cut(s) 414, 808, 1710, 1757, 1956, 3181, 3911
BssAI RCCGGY 2 cut(s) 3247, 3339
BssECI CCNNGG 8 cut(s) 1475, 2060, 2789, 3199, 3212, 3560, 3573, 3609
BssNAI GTATAC 1 cut(s) 1591
BssT1I CCWWGG 2 cut(s) 3212, 3560
Bst1107I GTATAC 1 cut(s) 1591
Bst2UI CCWGG 8 cut(s) 235, 513, 621, 1476, 2061, 2931, 3200, 3575
Bst4CI ACNGT 7 cut(s) 1694, 1845, 2199, 2374, 2773, 3331, 3655
Bst6I CTCTTC 5 cut(s) 369, 402, 3461, 3516, 3975
BstAFI CTTAAG 2 cut(s) 1573, 1907
BstBAI YACGTR 3 cut(s) 1664, 2426, 3889
BstC8I GCNNGC 3 cut(s) 1086, 2692, 3341
BstDSI CCRYGG 1 cut(s) 3212
BstENI CCTNNNNNAGG 1 cut(s) 624
BstF5I GGATG 9 cut(s) 454, 592, 1678, 1781, 1987, 1991, 2465, 3074, 3826
BstFNI CGCG 1 cut(s) 3897
BstHHI GCGC 2 cut(s) 1485, 2619
BstMAI GTCTC 7 cut(s) 399, 502, 1624, 2085, 2692, 3222, 3964
BstMCI CGRYCG 1 cut(s) 40
BstMWI GCNNNNNNNGC 5 cut(s) 1414, 1544, 1633, 2221, 3345
BstNI CCWGG 8 cut(s) 235, 513, 621, 1476, 2061, 2931, 3200, 3575
BstNSI RCATGY 5 cut(s) 1161, 1284, 1822, 3196, 3951
BstSFI CTRYAG 5 cut(s) 740, 1539, 2248, 2643, 3229
BstSLI GKGCMC 1 cut(s) 3591
BstUI CGCG 1 cut(s) 3897
BstV1I GCAGC 4 cut(s) 2093, 2601, 3001, 3215
BstV2I GAAGAC 5 cut(s) 49, 1513, 2048, 2258, 2787
BstX2I RGATCY 6 cut(s) 90, 1471, 2133, 3551, 3747, 3818
BstXI CCANNNNNNTGG 2 cut(s) 1757, 3003
BstYI RGATCY 6 cut(s) 90, 1471, 2133, 3551, 3747, 3818
BstZ17I GTATAC 1 cut(s) 1591
BstZI CGGCCG 1 cut(s) 37
Bsu15I ATCGAT 2 cut(s) 2204, 3856
BsuI GTATCC 1 cut(s) 3748
BsuRI GGCC 9 cut(s) 5, 39, 102, 2477, 3511, 3589, 3780, 3801, 3878
BsuTUI ATCGAT 2 cut(s) 2204, 3856
BtgI CCRYGG 1 cut(s) 3212
BtgZI GCGATG 2 cut(s) 166, 3394
BtrI CACGTC 2 cut(s) 3744, 3976
BtsCI GGATG 9 cut(s) 454, 592, 1678, 1781, 1987, 1991, 2465, 3074, 3826
BtsI GCAGTG 3 cut(s) 2178, 3456, 3506
BveI ACCTGC 1 cut(s) 2650
Cac8I GCNNGC 3 cut(s) 1086, 2692, 3341
CciI TCATGA 3 cut(s) 2022, 2749, 3430
CfoI GCGC 2 cut(s) 1485, 2619
Cfr10I RCCGGY 2 cut(s) 3247, 3339
Cfr13I GGNCC 6 cut(s) 2152, 2476, 3570, 3587, 3588, 3606
ClaI ATCGAT 2 cut(s) 2204, 3856
Csp6I GTAC 5 cut(s) 1066, 1364, 3102, 3127, 3720
CviQI GTAC 5 cut(s) 1066, 1364, 3102, 3127, 3720
DraI TTTAAA 1 cut(s) 3109
DraIII CACNNNGTG 1 cut(s) 965
EaeI YGGCCR 6 cut(s) 3, 37, 100, 3778, 3799, 3876
EagI CGGCCG 1 cut(s) 37
Eam1104I CTCTTC 5 cut(s) 369, 402, 3461, 3516, 3975
EarI CTCTTC 5 cut(s) 369, 402, 3461, 3516, 3975
Ecl136II GAGCTC 1 cut(s) 1379
EclXI CGGCCG 1 cut(s) 37
Eco130I CCWWGG 2 cut(s) 3212, 3560
Eco24I GRGCYC 2 cut(s) 1381, 3591
Eco31I GGTCTC 2 cut(s) 502, 3222
Eco47I GGWCC 3 cut(s) 2152, 3570, 3606
Eco52I CGGCCG 1 cut(s) 37
Eco53kI GAGCTC 1 cut(s) 1379
Eco57I CTGAAG 6 cut(s) 147, 1273, 1623, 2201, 3291, 3627
Eco72I CACGTG 2 cut(s) 1664, 3889
Eco88I CYCGRG 4 cut(s) 190, 645, 1101, 2524
EcoICRI GAGCTC 1 cut(s) 1379
EcoNI CCTNNNNNAGG 1 cut(s) 624
EcoO109I RGGNCCY 5 cut(s) 2152, 3570, 3587, 3588, 3606
EcoRII CCWGG 8 cut(s) 233, 511, 619, 1474, 2059, 2929, 3198, 3573
EcoT14I CCWWGG 2 cut(s) 3212, 3560
EcoT22I ATGCAT 3 cut(s) 1039, 1419, 3076
EcoT38I GRGCYC 2 cut(s) 1381, 3591
ErhI CCWWGG 2 cut(s) 3212, 3560
Esp3I CGTCTC 1 cut(s) 3964
FalI AAGNNNNNCTT 8 cut(s) 834, 866, 879, 911, 2719, 2751, 3612, 3644
FaqI GGGAC 3 cut(s) 972, 1727, 3556
FauI CCCGC 1 cut(s) 92
FbaI TGATCA 1 cut(s) 1513
FblI GTMKAC 2 cut(s) 477, 1590
Fnu4HI GCNGC 6 cut(s) 6, 411, 2107, 2615, 2990, 3229
FokI GGATG 9 cut(s) 461, 599, 1665, 1768, 1974, 1978, 2472, 3061, 3813
FriOI GRGCYC 2 cut(s) 1381, 3591
Fsp4HI GCNGC 6 cut(s) 6, 411, 2107, 2615, 2990, 3229
FspBI CTAG 7 cut(s) 945, 1115, 1178, 1244, 1271, 1350, 1827
FspI TGCGCA 1 cut(s) 2618
GlaI GCGC 2 cut(s) 1484, 2618
GluI GCNGC 6 cut(s) 6, 411, 2107, 2615, 2990, 3229
GsaI CCCAGC 3 cut(s) 430, 1444, 3582
GsuI CTGGAG 3 cut(s) 2816, 3164, 3946
HaeIII GGCC 9 cut(s) 5, 39, 102, 2477, 3511, 3589, 3780, 3801, 3878
HapII CCGG 4 cut(s) 436, 3248, 3340, 3751
HhaI GCGC 2 cut(s) 1485, 2619
Hin6I GCGC 2 cut(s) 1483, 2617
HinP1I GCGC 2 cut(s) 1483, 2617
HincII GTYRAC 3 cut(s) 478, 1697, 1875
HindII GTYRAC 3 cut(s) 478, 1697, 1875
HindIII AAGCTT 4 cut(s) 1231, 1605, 2327, 2435
HpaII CCGG 4 cut(s) 436, 3248, 3340, 3751
HphI GGTGA 8 cut(s) 268, 517, 803, 1784, 2490, 3134, 3392, 3502
Hpy166II GTNNAC 6 cut(s) 478, 1591, 1697, 1875, 3257, 3531
Hpy8I GTNNAC 6 cut(s) 478, 1591, 1697, 1875, 3257, 3531
Hpy99I CGWCG 7 cut(s) 80, 101, 158, 479, 482, 1939, 3745
HpyCH4III ACNGT 7 cut(s) 1694, 1845, 2199, 2374, 2773, 3331, 3655
HpyCH4IV ACGT 8 cut(s) 736, 1663, 2425, 3278, 3676, 3743, 3888, 3975
HpyF10VI GCNNNNNNNGC 5 cut(s) 1414, 1544, 1633, 2221, 3345
HpySE526I ACGT 8 cut(s) 736, 1663, 2425, 3278, 3676, 3743, 3888, 3975
HspAI GCGC 2 cut(s) 1483, 2617
KflI GGGWCCC 1 cut(s) 3570
KroI GCCGGC 1 cut(s) 3339
KroNI GCCGGC 1 cut(s) 3341
Ksp22I TGATCA 1 cut(s) 1513
LmnI GCTCC 4 cut(s) 250, 1133, 2064, 2161
Lsp1109I GCAGC 4 cut(s) 2093, 2601, 3001, 3215
LweI GCATC 8 cut(s) 442, 1534, 2734, 3061, 3083, 3357, 3417, 3836
MaeI CTAG 7 cut(s) 945, 1115, 1178, 1244, 1271, 1350, 1827
MaeII ACGT 8 cut(s) 736, 1663, 2425, 3278, 3676, 3743, 3888, 3975
MaeIII GTNAC 7 cut(s) 782, 1312, 1829, 1892, 2549, 2922, 3490
MfeI CAATTG 2 cut(s) 2184, 3143
MflI RGATCY 6 cut(s) 90, 1471, 2133, 3551, 3747, 3818
MhlI GDGCHC 4 cut(s) 1381, 2324, 3064, 3591
MlsI TGGCCA 3 cut(s) 3780, 3801, 3878
MluI ACGCGT 1 cut(s) 3895
MluNI TGGCCA 3 cut(s) 3780, 3801, 3878
MlyI GAGTC 5 cut(s) 143, 182, 2035, 2062, 3312
MmeI TCCRAC 4 cut(s) 23, 177, 514, 3165
Mox20I TGGCCA 3 cut(s) 3780, 3801, 3878
Mph1103I ATGCAT 3 cut(s) 1039, 1419, 3076
MroNI GCCGGC 1 cut(s) 3339
MroXI GAANNNNTTC 4 cut(s) 750, 1449, 2214, 3624
MscI TGGCCA 3 cut(s) 3780, 3801, 3878
MslI CAYNNNNRTG 9 cut(s) 1162, 1662, 1683, 1755, 2072, 2426, 3069, 3392, 3499
Msp20I TGGCCA 3 cut(s) 3780, 3801, 3878
MspA1I CMGCKG 1 cut(s) 2836
MspCI CTTAAG 2 cut(s) 1573, 1907
MspI CCGG 4 cut(s) 436, 3248, 3340, 3751
MunI CAATTG 2 cut(s) 2184, 3143
Mva1269I GAATGC 2 cut(s) 621, 1037
MvaI CCWGG 8 cut(s) 235, 513, 621, 1476, 2061, 2931, 3200, 3575
MvnI CGCG 1 cut(s) 3897
MwoI GCNNNNNNNGC 5 cut(s) 1414, 1544, 1633, 2221, 3345
NaeI GCCGGC 1 cut(s) 3341
NciI CCSGG 2 cut(s) 436, 3752
NcoI CCATGG 1 cut(s) 3212
NgoMIV GCCGGC 1 cut(s) 3339
NmuCI GTSAC 6 cut(s) 1312, 1829, 1892, 2549, 2922, 3490
NsbI TGCGCA 1 cut(s) 2618
NsiI ATGCAT 3 cut(s) 1039, 1419, 3076
NspI RCATGY 5 cut(s) 1161, 1284, 1822, 3196, 3951
PaeR7I CTCGAG 3 cut(s) 190, 645, 1101
PagI TCATGA 3 cut(s) 2022, 2749, 3430
PciI ACATGT 1 cut(s) 3192
PcsI WCGNNNNNNNCGW 3 cut(s) 153, 2662, 3894
PctI GAATGC 2 cut(s) 621, 1037
PdiI GCCGGC 1 cut(s) 3341
PdmI GAANNNNTTC 4 cut(s) 750, 1449, 2214, 3624
PfoI TCCNGGA 3 cut(s) 434, 2929, 3750
PkrI GCNGC 6 cut(s) 7, 412, 2108, 2616, 2991, 3230
PleI GAGTC 5 cut(s) 143, 182, 2034, 2061, 3311
PmaCI CACGTG 2 cut(s) 1664, 3889
PmlI CACGTG 2 cut(s) 1664, 3889
PpsI GAGTC 5 cut(s) 143, 182, 2034, 2061, 3311
Ppu21I YACGTR 3 cut(s) 1664, 2426, 3889
PpuMI RGGWCCY 3 cut(s) 2152, 3570, 3606
PscI ACATGT 1 cut(s) 3192
PshBI ATTAAT 1 cut(s) 1884
PsiI TTATAA 1 cut(s) 938
Psp124BI GAGCTC 1 cut(s) 1381
Psp5II RGGWCCY 3 cut(s) 2152, 3570, 3606
Psp6I CCWGG 8 cut(s) 233, 511, 619, 1474, 2059, 2929, 3198, 3573
PspCI CACGTG 2 cut(s) 1664, 3889
PspFI CCCAGC 3 cut(s) 426, 1440, 3578
PspGI CCWGG 8 cut(s) 233, 511, 619, 1474, 2059, 2929, 3198, 3573
PspOMI GGGCCC 1 cut(s) 3587
PspPI GGNCC 6 cut(s) 2152, 2476, 3570, 3587, 3588, 3606
PspPPI RGGWCCY 3 cut(s) 2152, 3570, 3606
PspXI VCTCGAGB 3 cut(s) 190, 645, 1101
PstI CTGCAG 4 cut(s) 744, 2252, 2647, 3233
PsuI RGATCY 6 cut(s) 90, 1471, 2133, 3551, 3747, 3818
PvuII CAGCTG 1 cut(s) 2836
RsaI GTAC 5 cut(s) 1067, 1365, 3103, 3128, 3721
RsaNI GTAC 5 cut(s) 1066, 1364, 3102, 3127, 3720
RseI CAYNNNNRTG 9 cut(s) 1162, 1662, 1683, 1755, 2072, 2426, 3069, 3392, 3499
SacI GAGCTC 1 cut(s) 1381
SalI GTCGAC 1 cut(s) 476
SatI GCNGC 6 cut(s) 6, 411, 2107, 2615, 2990, 3229
Sau96I GGNCC 6 cut(s) 2152, 2476, 3570, 3587, 3588, 3606
ScaI AGTACT 1 cut(s) 1365
SchI GAGTC 5 cut(s) 143, 182, 2035, 2062, 3312
SduI GDGCHC 4 cut(s) 1381, 2324, 3064, 3591
SfaNI GCATC 8 cut(s) 442, 1534, 2734, 3061, 3083, 3357, 3417, 3836
SfcI CTRYAG 5 cut(s) 740, 1539, 2248, 2643, 3229
Sfr274I CTCGAG 3 cut(s) 190, 645, 1101
SgrDI CGTCGACG 1 cut(s) 476
SinI GGWCC 3 cut(s) 2152, 3570, 3606
SlaI CTCGAG 3 cut(s) 190, 645, 1101
SmiMI CAYNNNNRTG 9 cut(s) 1162, 1662, 1683, 1755, 2072, 2426, 3069, 3392, 3499
SmlI CTYRAG 8 cut(s) 190, 645, 1078, 1101, 1573, 1907, 2117, 2725
SmoI CTYRAG 8 cut(s) 190, 645, 1078, 1101, 1573, 1907, 2117, 2725
SsiI CCGC 6 cut(s) 6, 85, 382, 411, 3360, 3663
SspMI CTAG 7 cut(s) 945, 1115, 1178, 1244, 1271, 1350, 1827
SstI GAGCTC 1 cut(s) 1381
StyI CCWWGG 2 cut(s) 3212, 3560
TaaI ACNGT 7 cut(s) 1694, 1845, 2199, 2374, 2773, 3331, 3655
TaiI ACGT 8 cut(s) 739, 1666, 2428, 3281, 3679, 3746, 3891, 3978
TaqII GACCGA 1 cut(s) 2781
TatI WGTACW 2 cut(s) 1363, 3126
TauI GCSGC 2 cut(s) 8, 413
TseFI GTSAC 6 cut(s) 1312, 1829, 1892, 2549, 2922, 3490
TseI GCWGC 4 cut(s) 2106, 2614, 2989, 3228
Tsp45I GTSAC 6 cut(s) 1312, 1829, 1892, 2549, 2922, 3490
TspGWI ACGGA 2 cut(s) 190, 627
Vha464I CTTAAG 2 cut(s) 1573, 1907
VpaK11BI GGWCC 3 cut(s) 2152, 3570, 3606
VspI ATTAAT 1 cut(s) 1884
XagI CCTNNNNNAGG 1 cut(s) 624
XapI RAATTY 5 cut(s) 992, 1209, 2940, 3444, 3724
XbaI TCTAGA 1 cut(s) 1270
XceI RCATGY 5 cut(s) 1161, 1284, 1822, 3196, 3951
XhoI CTCGAG 3 cut(s) 190, 645, 1101
XmiI GTMKAC 2 cut(s) 477, 1590
XmnI GAANNNNTTC 4 cut(s) 750, 1449, 2214, 3624
XspI CTAG 7 cut(s) 945, 1115, 1178, 1244, 1271, 1350, 1827
ZrmI AGTACT 1 cut(s) 1365
Zsp2I ATGCAT 3 cut(s) 1039, 1419, 3076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.