Rmu_sc0005410.1_g000018

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005410.1
OG0000067
Physical Location & Seq
Forward (+)
85372 .. 88841
3470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005410.1_g000018.1.cds

Sequence Viewer

Length: 3183 bp
atggtttcactattagaccttggtgaggacgatgttcgtgttgttggagtatatggactgcctggtatagggaaaacaaccattgctaaagctgtttatgacgaacttgtttgtcaatttgaacactattgctttcttgaaaatgtcaaggaaggtttcaagcataatggtgccatacatatgcgggaagaacttctatctaggatctttgagaaaagggtgcgcagtctaggcacttcgagtagaggttattccaagatgatgatggataggctgagtaagaaaaaagttctgcttgttcttgatgatgtagaaaactttaaccaaattgaagggttacttggaaggcagtattcatttggtggtgggagcagaatcattgtaaccactagggatatacaatcactcggtggagttaatgcgagatatactcccatgtttctaagcaatgatgaagctcttgaactttttatgcagtatgccttcaaaacaaacgaacctacgagagagtatgatcctctctcaaggcgtgctgtaaaatatgctcaaggtctacctttagcactcacagttttaggagctagtcttgctggcaaaagtatacgtgagtggaaagatgagctagataaaataaaggtaatcccgaataaggaaattcagggtgtgcttgcaacaagctttgatggactggatccttcacagcgggacatctttctagacatcgcatgtttctttagaggaatggacaaaagctatgtaatcaaaattctggaaaattgtggcttctatccccacagtggattacgagcactatgtgatagagctctcttaactatctcagatgacaataggcttgaaatgcatgatttaatacaggatatgggttggcaaattgtccgccgacaatctattaaaatgcctgggaagcgcagtcgtttgtggacttatgaagacgtggatcatgtattagctcaaaatacggctacagaagcagttgaaggcatattgctagacttgtcgaagtcaaaaaacgttgacatagatgctgaagcttttgttaaaatgacgaaactaagactgctcataatcggttataattaccactacatagacttcaatgtgaaagattttccatcagggcagttgtaccatccaggagatgagtgtaaacaacatgtgaatggagaagttaaatttctttctcatgacctgaggtatctcatgtggtatggatgccccctaaagtttttgccatctaattttcaaccacagaatcttggtgaccttgacatgcgttatagccagattgaacaactttgggaaggggccaagcccttcaaaaagttgaaattcatcaatttgagccactcaatttaccttatcacaacccctgatttcactgaagcgacaaatcttgaaacactagttcttgaaggttgttcaagtttacgtggtgttcacgcatccatttcagctcttaaagacctcgtattcttgtgcctaaaaggttgcaaaaaacttaagagtcttccaagcagcatttgctttgagtctcttaaaacccttgatctctctggctgctcaagtcttgaaaagtttccagatatttcaggaattatggacaaccgattggaactttatctaaatgagactgcaattgaagaactgccgtcatcaattgaaagacttaaagggcttgtggtattaaatttaggaaactgcagaaacctcatccgtcttccggacaacatctgtaagttggtacgtcttacagatctcaatgtctctgggtgctcaaagcttgatcatttgcctgacaacttggggaatttagaatgcttggtgagccttaaagtagaaggaagtggtattataggactcccattctccatcttacgcttgaataagctcaaaaaattgtcatgtgatggctgtaaaaaaatggagatgcccctttcatcatggtcatcatcaattgaagaaatttcttgttactctggtctggtacatcttcaattaagtgactgcaatctgttggaattatccgatactattgctgatttatcttcattaaaaacattagatctgcgaagaaacaacaacttggaaagcttacctgcaacaatgaatcaacttcgtcatttgacacatcttgaactggaaggttgcaagagactgaaatcaataccagagctttcgtcaagtataagttacatagatgcacatgactgcaaagctttggtaaatgttgcaaaaccaaggcgtcggcgggctaggactctttgcttcacattttctaattgcctccaactggtacaaacaaatctatttagcgatattgtggaagctcattctcatgaccaggatatgcgagcccttccctttaatatttctcttcctggaagtgcaattcctaaatggttcgtccatcgagataggggatcatcaataactgtacaactacccacaaactggtttgataattggtttttggggtttgctatatgtgctgttagtgacttcaagggtgctcatcaagcatctgatctatctgctcgatgttattgtaccttcagaggaaatcatggtgtatacagtttcaattttactttggtcgattggggtgtcataagagacagattcctggaatcagatcacatgttcctcggatatgtgccatggtctgaatatcgctttattcaagaaggaaagtgcgtcggtgaacgctattacacggaggccacatttaagattgtattacaaattggagtccatgtacttggtttcaggacagtaacgccccattgcatcacaagctgcggagttcgtttgtttcatggtagttacatgagcgagaaaagtccaagcattactgaacgtgacaggttgaagagaaggagagaaagagaggggggggacgaggtgtctccgtcaggactgaagctgttcccggatctagaactgcttcacacaagggaaggagccacagaggtgtctcagccggtaccgaagccgttccggccgcatctagaaccggtcgccataagggaaggagccacagaggggcaggagtcctcggttggcgaaaggtcttcagcagggcaagggtcctctctgccttcagtagcaacatgtgaaagccataccactcggaacagtcgtgcactccttgaccttatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1060

Amino Acids

119.54

Weight (kDa)

6.86

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1095
Acc16I TGCGCA 1 cut(s) 224
Acc36I ACCTGC 1 cut(s) 2125
Acc65I GGTACC 1 cut(s) 3004
AccB1I GGYRCC 2 cut(s) 170, 3004
AccB7I CCANNNNNTGG 1 cut(s) 2472
AccI GTMKAC 3 cut(s) 553, 601, 2592
AccIII TCCGGA 1 cut(s) 1741
AciI CCGC 6 cut(s) 184, 703, 898, 2269, 2820, 3023
AclI AACGTT 1 cut(s) 1032
AclWI GGATC 7 cut(s) 212, 509, 686, 699, 966, 2449, 2961
AcoI YGGCCR 1 cut(s) 3020
AcsI RAATTY 7 cut(s) 654, 765, 1193, 1349, 1708, 1828, 1983
AcuI CTGAAG 6 cut(s) 1068, 1422, 2557, 2960, 3078, 3105
AcyI GRCGYC 1 cut(s) 2263
AdeI CACNNNGTG 2 cut(s) 410, 815
AfaI GTAC 8 cut(s) 1148, 1764, 2007, 2316, 2457, 2569, 2778, 3006
AfiI CCNNNNNNNGG 8 cut(s) 25, 68, 649, 797, 1741, 2311, 2472, 3063
AflII CTTAAG 1 cut(s) 1520
AflIII ACRYGT 3 cut(s) 1174, 2658, 3131
AgeI ACCGGT 1 cut(s) 3034
AhdI GACNNNNNGTC 1 cut(s) 2923
AhlI ACTAGT 1 cut(s) 1423
AjiI CACGTC 1 cut(s) 955
AjnI CCWGG 6 cut(s) 61, 919, 1153, 2361, 2398, 2643
AjuI GAANNNNNNNTTGG 2 cut(s) 324, 356
AleI CACNNNNGTG 1 cut(s) 2990
Alw21I GWGCWC 5 cut(s) 811, 826, 1796, 2533, 3166
Alw26I GTCTC 7 cut(s) 1557, 1643, 1789, 2167, 2628, 2931, 3000
Alw44I GTGCAC 1 cut(s) 3162
AlwI GGATC 7 cut(s) 212, 509, 686, 699, 966, 2449, 2961
Aor13HI TCCGGA 1 cut(s) 1741
AoxI GGCC 3 cut(s) 1326, 2739, 3020
ApaLI GTGCAC 1 cut(s) 3162
ApeKI GCWGC 3 cut(s) 1536, 1578, 2817
ApoI RAATTY 7 cut(s) 654, 765, 1193, 1349, 1708, 1828, 1983
AsiGI ACCGGT 1 cut(s) 3034
Asp700I GAANNNNTTC 5 cut(s) 192, 1596, 2945, 2964, 3014
Asp718I GGTACC 1 cut(s) 3004
AspLEI GCGC 2 cut(s) 225, 930
AspS9I GGNCC 2 cut(s) 1326, 3108
AsuC2I CCSGG 1 cut(s) 2951
AsuHPI GGTGA 4 cut(s) 35, 1292, 1855, 2732
AvaII GGWCC 1 cut(s) 3108
AxyI CCTNAGG 1 cut(s) 1211
BaeGI GKGCMC 1 cut(s) 3166
BamHI GGATCC 1 cut(s) 691
BanI GGYRCC 2 cut(s) 170, 3004
BanII GRGCYC 2 cut(s) 826, 2377
BbsI GAAGAC 4 cut(s) 957, 1520, 1730, 3084
Bbv12I GWGCWC 5 cut(s) 811, 826, 1796, 2533, 3166
BbvI GCAGC 3 cut(s) 1548, 1565, 2804
BccI CCATC 8 cut(s) 259, 677, 1141, 1158, 1261, 1898, 1922, 2436
BceAI ACGGC 3 cut(s) 996, 1654, 2998
BcgI CGANNNNNNTGC 2 cut(s) 2036, 2070
BciT130I CCWGG 6 cut(s) 63, 921, 1155, 2363, 2400, 2645
BclI TGATCA 1 cut(s) 1804
BcnI CCSGG 1 cut(s) 2951
BcoDI GTCTC 7 cut(s) 1557, 1643, 1789, 2167, 2628, 2931, 3000
BcuI ACTAGT 1 cut(s) 1423
BfmI CTRYAG 2 cut(s) 984, 1720
BfrI CTTAAG 1 cut(s) 1520
BfuAI ACCTGC 1 cut(s) 2125
BglI GCCNNNNNGGC 1 cut(s) 3019
BglII AGATCT 2 cut(s) 1774, 2083
BisI GCNGC 4 cut(s) 1537, 1579, 2818, 3023
BlsI GCNGC 4 cut(s) 1538, 1580, 2819, 3024
Bme1390I CCNGG 7 cut(s) 63, 921, 1155, 2363, 2400, 2645, 2951
Bme18I GGWCC 1 cut(s) 3108
BmeRI GACNNNNNGTC 1 cut(s) 2923
BmgBI CACGTC 1 cut(s) 955
BmgT120I GGNCC 2 cut(s) 1326, 3108
BmiI GGNNCC 7 cut(s) 172, 693, 1327, 2983, 3006, 3055, 3109
BmrFI CCNGG 7 cut(s) 63, 921, 1155, 2363, 2400, 2645, 2951
BmsI GCATC 8 cut(s) 1033, 1223, 1472, 1938, 2209, 2549, 2817, 3034
BpiI GAAGAC 4 cut(s) 957, 1520, 1730, 3084
BplI GAGNNNNNCTC 2 cut(s) 415, 447
BpuEI CTTGAG 3 cut(s) 508, 531, 1567
BpuMI CCSGG 1 cut(s) 2951
BsaAI YACGTR 2 cut(s) 605, 1451
BsaHI GRCGYC 1 cut(s) 2263
BsaJI CCNNGG 6 cut(s) 19, 920, 2258, 2665, 2678, 3075
BsaWI WCCGGW 2 cut(s) 1741, 3034
BsaXI ACNNNNNCTCC 2 cut(s) 2760, 2790
Bsc4I CCNNNNNNNGG 8 cut(s) 25, 68, 649, 797, 1741, 2311, 2472, 3063
Bse118I RCCGGY 2 cut(s) 3001, 3034
Bse1I ACTGG 4 cut(s) 693, 2163, 2316, 2477
Bse21I CCTNAGG 1 cut(s) 1211
Bse3DI GCAATG 3 cut(s) 81, 454, 2803
BseAI TCCGGA 1 cut(s) 1741
BseBI CCWGG 6 cut(s) 63, 921, 1155, 2363, 2400, 2645
BseDI CCNNGG 6 cut(s) 19, 920, 2258, 2665, 2678, 3075
BseGI GGATG 4 cut(s) 1150, 1238, 1463, 1731
BseLI CCNNNNNNNGG 8 cut(s) 25, 68, 649, 797, 1741, 2311, 2472, 3063
BseMI GCAATG 3 cut(s) 81, 454, 2803
BseMII CTCAG 4 cut(s) 266, 852, 1202, 3011
BseNI ACTGG 4 cut(s) 693, 2163, 2316, 2477
BseSI GKGCMC 1 cut(s) 3166
BseX3I CGGCCG 1 cut(s) 3020
BseXI GCAGC 3 cut(s) 1548, 1565, 2804
Bsh1285I CGRYCG 2 cut(s) 3023, 3039
BshFI GGCC 3 cut(s) 1328, 2741, 3022
BshNI GGYRCC 2 cut(s) 170, 3004
BshTI ACCGGT 1 cut(s) 3034
BsiEI CGRYCG 2 cut(s) 3023, 3039
BsiHKAI GWGCWC 5 cut(s) 811, 826, 1796, 2533, 3166
BsiSI CCGG 5 cut(s) 1742, 2951, 3002, 3019, 3035
BslFI GGGAC 2 cut(s) 719, 2930
BslI CCNNNNNNNGG 8 cut(s) 25, 68, 649, 797, 1741, 2311, 2472, 3063
BsmAI GTCTC 7 cut(s) 1557, 1643, 1789, 2167, 2628, 2931, 3000
BsmFI GGGAC 2 cut(s) 719, 2930
BsmI GAATGC 1 cut(s) 1841
BsnI GGCC 3 cut(s) 1328, 2741, 3022
Bsp1286I GDGCHC 6 cut(s) 811, 826, 1796, 2377, 2533, 3166
Bsp13I TCCGGA 1 cut(s) 1741
Bsp1407I TGTACA 1 cut(s) 2455
Bsp19I CCATGG 1 cut(s) 2678
BspACI CCGC 6 cut(s) 184, 703, 898, 2269, 2820, 3023
BspANI GGCC 3 cut(s) 1328, 2741, 3022
BspCNI CTCAG 4 cut(s) 267, 851, 1203, 3010
BspEI TCCGGA 1 cut(s) 1741
BspHI TCATGA 2 cut(s) 1204, 2356
BspLI GGNNCC 7 cut(s) 172, 693, 1327, 2983, 3006, 3055, 3109
BspMAI CTGCAG 1 cut(s) 1724
BspMI ACCTGC 1 cut(s) 2125
BspPI GGATC 7 cut(s) 212, 509, 686, 699, 966, 2449, 2961
BspT107I GGYRCC 2 cut(s) 170, 3004
BspTI CTTAAG 1 cut(s) 1520
BsrDI GCAATG 3 cut(s) 81, 454, 2803
BsrFI RCCGGY 2 cut(s) 3001, 3034
BsrGI TGTACA 1 cut(s) 2455
BsrI ACTGG 4 cut(s) 693, 2163, 2316, 2477
BssAI RCCGGY 2 cut(s) 3001, 3034
BssECI CCNNGG 6 cut(s) 19, 920, 2258, 2665, 2678, 3075
BssNAI GTATAC 2 cut(s) 602, 2593
BssNI GRCGYC 1 cut(s) 2263
BssT1I CCWWGG 3 cut(s) 19, 2258, 2678
Bst1107I GTATAC 2 cut(s) 602, 2593
Bst2UI CCWGG 6 cut(s) 63, 921, 1155, 2363, 2400, 2645
Bst4CI ACNGT 6 cut(s) 571, 797, 2455, 2597, 2794, 3158
Bst6I CTCTTC 2 cut(s) 2400, 2885
BstACI GRCGYC 1 cut(s) 2263
BstAFI CTTAAG 1 cut(s) 1520
BstAPI GCANNNNNTGC 1 cut(s) 1542
BstAUI TGTACA 1 cut(s) 2455
BstBAI YACGTR 2 cut(s) 605, 1451
BstC8I GCNNGC 5 cut(s) 531, 592, 669, 2271, 2373
BstDEI CTNAG 6 cut(s) 275, 443, 838, 1073, 1211, 2997
BstDSI CCRYGG 1 cut(s) 2678
BstEII GGTNACC 1 cut(s) 1280
BstENI CCTNNNNNAGG 2 cut(s) 23, 66
BstF5I GGATG 4 cut(s) 1150, 1238, 1463, 1731
BstHHI GCGC 2 cut(s) 225, 930
BstMAI GTCTC 7 cut(s) 1557, 1643, 1789, 2167, 2628, 2931, 3000
BstMCI CGRYCG 2 cut(s) 3023, 3039
BstNI CCWGG 6 cut(s) 63, 921, 1155, 2363, 2400, 2645
BstNSI RCATGY 5 cut(s) 729, 1178, 1294, 2662, 3135
BstPI GGTNACC 1 cut(s) 1280
BstSCI CCNGG 7 cut(s) 61, 919, 1153, 2361, 2398, 2643, 2949
BstSFI CTRYAG 2 cut(s) 984, 1720
BstSLI GKGCMC 1 cut(s) 3166
BstV1I GCAGC 3 cut(s) 1548, 1565, 2804
BstV2I GAAGAC 4 cut(s) 957, 1520, 1730, 3084
BstX2I RGATCY 5 cut(s) 204, 691, 1774, 2083, 2953
BstXI CCANNNNNNTGG 1 cut(s) 2780
BstYI RGATCY 5 cut(s) 204, 691, 1774, 2083, 2953
BstZ17I GTATAC 2 cut(s) 602, 2593
BstZI CGGCCG 1 cut(s) 3020
Bsu36I CCTNAGG 1 cut(s) 1211
BsuRI GGCC 3 cut(s) 1328, 2741, 3022
BtgI CCRYGG 1 cut(s) 2678
BtgZI GCGATG 1 cut(s) 706
BtrI CACGTC 1 cut(s) 955
BtsCI GGATG 4 cut(s) 1150, 1238, 1463, 1731
BtsIMutI CAGTG 2 cut(s) 802, 1398
BveI ACCTGC 1 cut(s) 2125
Cac8I GCNNGC 5 cut(s) 531, 592, 669, 2271, 2373
CciI TCATGA 2 cut(s) 1204, 2356
CfoI GCGC 2 cut(s) 225, 930
Cfr10I RCCGGY 2 cut(s) 3001, 3034
Cfr13I GGNCC 2 cut(s) 1326, 3108
CseI GACGC 2 cut(s) 2252, 2704
Csp6I GTAC 8 cut(s) 1147, 1763, 2006, 2315, 2456, 2568, 2777, 3005
CspAI ACCGGT 1 cut(s) 3034
CviQI GTAC 8 cut(s) 1147, 1763, 2006, 2315, 2456, 2568, 2777, 3005
DdeI CTNAG 6 cut(s) 275, 443, 838, 1073, 1211, 2997
DraIII CACNNNGTG 2 cut(s) 410, 815
DriI GACNNNNNGTC 1 cut(s) 2923
EaeI YGGCCR 1 cut(s) 3020
EagI CGGCCG 1 cut(s) 3020
Eam1104I CTCTTC 2 cut(s) 2400, 2885
Eam1105I GACNNNNNGTC 1 cut(s) 2923
EarI CTCTTC 2 cut(s) 2400, 2885
EciI GGCGGA 1 cut(s) 887
Ecl136II GAGCTC 1 cut(s) 824
EclXI CGGCCG 1 cut(s) 3020
Eco130I CCWWGG 3 cut(s) 19, 2258, 2678
Eco24I GRGCYC 2 cut(s) 826, 2377
Eco47I GGWCC 1 cut(s) 3108
Eco52I CGGCCG 1 cut(s) 3020
Eco53kI GAGCTC 1 cut(s) 824
Eco57I CTGAAG 6 cut(s) 1068, 1422, 2557, 2960, 3078, 3105
Eco81I CCTNAGG 1 cut(s) 1211
Eco91I GGTNACC 1 cut(s) 1280
EcoICRI GAGCTC 1 cut(s) 824
EcoNI CCTNNNNNAGG 2 cut(s) 23, 66
EcoO109I RGGNCCY 1 cut(s) 3108
EcoO65I GGTNACC 1 cut(s) 1280
EcoRII CCWGG 6 cut(s) 61, 919, 1153, 2361, 2398, 2643
EcoT14I CCWWGG 3 cut(s) 19, 2258, 2678
EcoT22I ATGCAT 1 cut(s) 864
EcoT38I GRGCYC 2 cut(s) 826, 2377
ErhI CCWWGG 3 cut(s) 19, 2258, 2678
FalI AAGNNNNNCTT 4 cut(s) 279, 311, 324, 356
FaqI GGGAC 2 cut(s) 719, 2930
FauI CCCGC 3 cut(s) 177, 696, 2262
FauNDI CATATG 1 cut(s) 180
FbaI TGATCA 1 cut(s) 1804
FblI GTMKAC 3 cut(s) 553, 601, 2592
Fnu4HI GCNGC 4 cut(s) 1537, 1579, 2818, 3023
FokI GGATG 4 cut(s) 1137, 1245, 1450, 1718
FriOI GRGCYC 2 cut(s) 826, 2377
Fsp4HI GCNGC 4 cut(s) 1537, 1579, 2818, 3023
FspI TGCGCA 1 cut(s) 224
GlaI GCGC 2 cut(s) 224, 929
GluI GCNGC 4 cut(s) 1537, 1579, 2818, 3023
HaeIII GGCC 3 cut(s) 1328, 2741, 3022
HapII CCGG 5 cut(s) 1742, 2951, 3002, 3019, 3035
HgaI GACGC 2 cut(s) 2252, 2704
HhaI GCGC 2 cut(s) 225, 930
Hin1I GRCGYC 1 cut(s) 2263
Hin6I GCGC 2 cut(s) 223, 928
HinP1I GCGC 2 cut(s) 223, 928
HincII GTYRAC 1 cut(s) 1036
HindII GTYRAC 1 cut(s) 1036
HindIII AAGCTT 5 cut(s) 676, 1050, 1799, 2110, 2235
HpaII CCGG 5 cut(s) 1742, 2951, 3002, 3019, 3035
HphI GGTGA 4 cut(s) 35, 1292, 1855, 2732
Hpy188I TCNGA 8 cut(s) 841, 2047, 2545, 2576, 2653, 2669, 2686, 3153
Hpy99I CGWCG 2 cut(s) 2268, 2720
HpyCH4III ACNGT 6 cut(s) 571, 797, 2455, 2597, 2794, 3158
HpyCH4IV ACGT 6 cut(s) 604, 954, 1032, 1450, 1765, 2878
HpyF3I CTNAG 6 cut(s) 275, 443, 838, 1073, 1211, 2997
HpySE526I ACGT 6 cut(s) 604, 954, 1032, 1450, 1765, 2878
Hsp92I GRCGYC 1 cut(s) 2263
HspAI GCGC 2 cut(s) 223, 928
Kpn2I TCCGGA 1 cut(s) 1741
KpnI GGTACC 1 cut(s) 3008
Ksp22I TGATCA 1 cut(s) 1804
LmnI GCTCC 4 cut(s) 369, 578, 2981, 3053
Lsp1109I GCAGC 3 cut(s) 1548, 1565, 2804
LweI GCATC 8 cut(s) 1033, 1223, 1472, 1938, 2209, 2549, 2817, 3034
MaeII ACGT 6 cut(s) 604, 954, 1032, 1450, 1765, 2878
MfeI CAATTG 3 cut(s) 1656, 1677, 1974
MflI RGATCY 5 cut(s) 204, 691, 1774, 2083, 2953
MhlI GDGCHC 6 cut(s) 811, 826, 1796, 2377, 2533, 3166
MlyI GAGTC 6 cut(s) 1534, 1559, 1872, 2272, 2778, 3080
MmeI TCCRAC 3 cut(s) 25, 2016, 2332
Mph1103I ATGCAT 1 cut(s) 864
MroI TCCGGA 1 cut(s) 1741
MroXI GAANNNNTTC 5 cut(s) 192, 1596, 2945, 2964, 3014
MslI CAYNNNNRTG 5 cut(s) 168, 179, 1179, 2355, 2990
MspA1I CMGCKG 1 cut(s) 703
MspCI CTTAAG 1 cut(s) 1520
MspI CCGG 5 cut(s) 1742, 2951, 3002, 3019, 3035
MspR9I CCNGG 7 cut(s) 63, 921, 1155, 2363, 2400, 2645, 2951
MunI CAATTG 3 cut(s) 1656, 1677, 1974
Mva1269I GAATGC 1 cut(s) 1841
MvaI CCWGG 6 cut(s) 63, 921, 1155, 2363, 2400, 2645
NciI CCSGG 1 cut(s) 2951
NcoI CCATGG 1 cut(s) 2678
NdeI CATATG 1 cut(s) 180
NlaIV GGNNCC 7 cut(s) 172, 693, 1327, 2983, 3006, 3055, 3109
NmuCI GTSAC 4 cut(s) 1280, 2021, 2516, 2879
NsbI TGCGCA 1 cut(s) 224
NsiI ATGCAT 1 cut(s) 864
NspI RCATGY 5 cut(s) 729, 1178, 1294, 2662, 3135
OliI CACNNNNGTG 1 cut(s) 2990
PagI TCATGA 2 cut(s) 1204, 2356
PciI ACATGT 3 cut(s) 1174, 2658, 3131
PcsI WCGNNNNNNNCGW 1 cut(s) 3157
PctI GAATGC 1 cut(s) 1841
PdmI GAANNNNTTC 5 cut(s) 192, 1596, 2945, 2964, 3014
PfeI GAWTC 5 cut(s) 375, 1273, 2128, 2640, 2648
PflMI CCANNNNNTGG 1 cut(s) 2472
PfoI TCCNGGA 4 cut(s) 1153, 2398, 2643, 2949
PinAI ACCGGT 1 cut(s) 3034
PkrI GCNGC 4 cut(s) 1538, 1580, 2819, 3024
PleI GAGTC 6 cut(s) 1533, 1558, 1872, 2272, 2777, 3079
PpsI GAGTC 6 cut(s) 1533, 1558, 1872, 2272, 2777, 3079
Ppu21I YACGTR 2 cut(s) 605, 1451
PpuMI RGGWCCY 1 cut(s) 3108
PscI ACATGT 3 cut(s) 1174, 2658, 3131
PsiI TTATAA 1 cut(s) 1095
Psp124BI GAGCTC 1 cut(s) 826
Psp1406I AACGTT 1 cut(s) 1032
Psp5II RGGWCCY 1 cut(s) 3108
Psp6I CCWGG 6 cut(s) 61, 919, 1153, 2361, 2398, 2643
PspEI GGTNACC 1 cut(s) 1280
PspGI CCWGG 6 cut(s) 61, 919, 1153, 2361, 2398, 2643
PspN4I GGNNCC 7 cut(s) 172, 693, 1327, 2983, 3006, 3055, 3109
PspPI GGNCC 2 cut(s) 1326, 3108
PspPPI RGGWCCY 1 cut(s) 3108
PstI CTGCAG 1 cut(s) 1724
PsuI RGATCY 5 cut(s) 204, 691, 1774, 2083, 2953
RsaI GTAC 8 cut(s) 1148, 1764, 2007, 2316, 2457, 2569, 2778, 3006
RsaNI GTAC 8 cut(s) 1147, 1763, 2006, 2315, 2456, 2568, 2777, 3005
RseI CAYNNNNRTG 5 cut(s) 168, 179, 1179, 2355, 2990
SacI GAGCTC 1 cut(s) 826
SatI GCNGC 4 cut(s) 1537, 1579, 2818, 3023
Sau96I GGNCC 2 cut(s) 1326, 3108
SchI GAGTC 6 cut(s) 1534, 1559, 1872, 2272, 2778, 3080
ScrFI CCNGG 7 cut(s) 63, 921, 1155, 2363, 2400, 2645, 2951
SduI GDGCHC 6 cut(s) 811, 826, 1796, 2377, 2533, 3166
SfaNI GCATC 8 cut(s) 1033, 1223, 1472, 1938, 2209, 2549, 2817, 3034
SfcI CTRYAG 2 cut(s) 984, 1720
SinI GGWCC 1 cut(s) 3108
SmiMI CAYNNNNRTG 5 cut(s) 168, 179, 1179, 2355, 2990
SmlI CTYRAG 4 cut(s) 523, 546, 1520, 1582
SmoI CTYRAG 4 cut(s) 523, 546, 1520, 1582
SpeI ACTAGT 1 cut(s) 1423
SsiI CCGC 6 cut(s) 184, 703, 898, 2269, 2820, 3023
SspI AATATT 1 cut(s) 2389
SstI GAGCTC 1 cut(s) 826
StyD4I CCNGG 7 cut(s) 61, 919, 1153, 2361, 2398, 2643, 2949
StyI CCWWGG 3 cut(s) 19, 2258, 2678
TaaI ACNGT 6 cut(s) 571, 797, 2455, 2597, 2794, 3158
TaiI ACGT 6 cut(s) 607, 957, 1035, 1453, 1768, 2881
TaqI TCGA 5 cut(s) 239, 1019, 2431, 2557, 2616
TatI WGTACW 2 cut(s) 2455, 2776
TauI GCSGC 1 cut(s) 3025
TfiI GAWTC 5 cut(s) 375, 1273, 2128, 2640, 2648
TscAI CASTG 2 cut(s) 802, 1405
TseFI GTSAC 4 cut(s) 1280, 2021, 2516, 2879
TseI GCWGC 3 cut(s) 1536, 1578, 2817
Tsp45I GTSAC 4 cut(s) 1280, 2021, 2516, 2879
TspDTI ATGAA 8 cut(s) 345, 468, 963, 1342, 1947, 2058, 2141, 2825
TspGWI ACGGA 3 cut(s) 1724, 2750, 2919
TspRI CASTG 2 cut(s) 802, 1405
Van91I CCANNNNNTGG 1 cut(s) 2472
Vha464I CTTAAG 1 cut(s) 1520
VneI GTGCAC 1 cut(s) 3162
VpaK11BI GGWCC 1 cut(s) 3108
XagI CCTNNNNNAGG 2 cut(s) 23, 66
XapI RAATTY 7 cut(s) 654, 765, 1193, 1349, 1708, 1828, 1983
XbaI TCTAGA 3 cut(s) 715, 2956, 3028
XceI RCATGY 5 cut(s) 729, 1178, 1294, 2662, 3135
XcmI CCANNNNNNNNNTGG 1 cut(s) 262
XmiI GTMKAC 3 cut(s) 553, 601, 2592
XmnI GAANNNNTTC 5 cut(s) 192, 1596, 2945, 2964, 3014
Zsp2I ATGCAT 1 cut(s) 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.