Rmu_sc0000827.1_g000022

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000827.1
OG0000067
Physical Location & Seq
Forward (+)
94386 .. 99140
4755 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000827.1_g000022.1.cds

Sequence Viewer

Length: 3642 bp
atggctggtgcttcttcatcatccggaggcagtctcaaatacgacgttttcatcaatttcagaggcgaagacacacgtaggtgtttcgtctgccatctttacctagctctgaaacagaaaacactcgagcccttcattgattccaaagagcttagaacaggcgacggcctttcggcgctattcaaagccattcaagactcgaggctttcgattgttgttttctctaaacattatgcctcttccacatggtgcttagaagaactggtccacatcctggagtgcatggataggcggggccagattgtggtgcccatattctatgaagtaaatccctcccatatttgcaaccttgaggaaagctttgcaaaagcttttgcagaacatgaaagcaattccaagatcgacaaagaagagttggagagctggaagtccagtttaaagagagccgccaatatatctggctaccactccacaaattatcgggatgatagcgagcttatcggcagcattgtagaagacatttttatgaaattgttccccaactcgccaactaaaaccgatggcttggttggaatggattctcacataaataaaatggataggctattacaactgggggtggatgattctcgcattgttggaatctggggtatgggcgggatcggcaagtcaaccatcgctcgagctgtttatgaaaaaatcgctcctcaatttgaacactcgtgctttcttgataatgtgaaggagggtttcctcacaaaaaatggtggaggacaaatggttgaagaacttctatccagaattctaaaggtaaacaatcgacgcactttggatggaggcctgaataagatgagggaaagacttggtaagaaaagaattcttcttgttcttgatgatgtggacaatcaggaccaaatcgataccttgattggagaaaaacctacatttggtggtgggagcagaattattataacaactagagataagaagttattaggtggatgttcgatatacaagcccaagttgttgactgatgaagaagctcttgaactctttagcaagcatgctttcagaacaaacaaacgctcagagagttacgatgctatgtcaaggcgtgcagtaaaatacgcctatggtcttcctttggcactcaaagtcttgggagctttgcttgataacaggagtgaaccggagtggaaatatgagttggagaagataaagaaaatcccagtcacgtctcttgatgataaaattagacgtgtgctttgggcaagctatgatggattgcatgaatgtgagaagaacatatttctagatgttgcatgtttctttaagggaaagaacaaagattttgtaacaaagattcatgaaagttgtggctttttcccccagattggactaacagttttggcagaaagatctcttgtaactgtctcatcatggggtgataaactagagatgcatgatttgatacaggaagtgggtagggaaatcgtccgccaagaatctactaaagatccaggaatgcgtagtagattatgggactatgaagatgttcgccaagtgttagctcaaaatacggctactgaagcagttgaaggaataatcctagacttgtcaaagttaaaaaaggtacacgtaaatgatgaagtttttgttagcatgaggagactaaagctactccagctttctgaatatcattatggaacggaatatgattatgaaattgcatggcgaaaagaagattatgaaccctgtgtacaagacatgagtgagaacttaaagtttctttcgaatgagttgagagttcttgtgtggcatggatgcccactaaagtctttcccaagtgattttctaccagaaaaacttatttacgtcgacatgtgttatagtcgtattgaacaactttgggaagaaaccaagcctttgttggaatacatagatttaagttgctcaaagtatctaatcaaaacccccgacttcactgaggcaacaagtcttgcgaaactgaagcttaaaggatgtacaagtttactggaggttcacccatccatttcagctcttcaaagcctcgttgagttggacctacgaggctgcacaaatctgaagaaatttccaaagatgtccagaaatatgaagaagctaagtgaactttacctaaatgagactgcaattacagaaatgccctcatcaattgatgatcttttcgggctgcagattttgagcctcgctggttgtagagaactctggagccttccgagtagaattcacatgaaatctgtgaaatctcttgatttatctgactgctcgaaacttacgaagtttccagagatttcagaagtaatggagaagctatgggagcttaaattagacagaacatcaattgaagaacttccctcatctattagtaatcttacggggcttctagtgttaagtttgacagactgcacaagccttgtctgtcttccggacaatatctgcaatttggcatgccttcgttttctctatctgactgggtgctctaacctttcaaagttgcctgagaactttgggaatttgaaatccttgtggaaggaagaagacggccttggtctggagggaagtggtataaaacaacttccattctctgtcttgtgtatggaatcattttcatgtaatggatgtaaagagatgacggcgcccttttcctcttggccaacatggaaccaccaaaatcctagttactctgttctcaccgatcttgatttaagtgactgcaacctcttgcaattatcggatggtattgctcatttatcttcattggaaaaattagacttatccggaaacaacaatttggaaagtttacctgcagcaatgaattacctgcgtaaattgatatggctcaatctgagaggttgcgagagtctgaaatcaataccagagctttcatcgagtatacaagatatcattgcccgggattgcacatctttggagacagtttcagcaccacagcctccgtacgctgagaacatttccttccaattttttaattgcttcaagttggtgaacaataccaatatatttatagatattgtggaacgtgctactcataatcaggattatgaatgctatatgtctcttcctggaagtgatattccaaattggttcaaccatcagagtggagggtcttccgtaactgtgccgttaccacccaattggctcgcaaacaagtttctggggttcgctatatgcgccatcagtagcttgcagggtgatgattatataacaactcttgaatctgcttactgtttttgtaccttaaaagcaaatgatggtcaggacaatttctgttttcctctgtcctattatcttagtggaactggcaagccccctgagttgggtcatatgttgctgggatatacaaatttccgaagtttcggaaagtggcataatgaacgcaaatacaccgaggccagatttaggatagtgatggcggactatcaagtcatccgagatgattgggaagattgcacttgggtcacaaggtgcggggttcggttattcttagagttgaatccaccattctctggggaccttatttgcaatttggaaaatagagaaacagagacagggaggaagagaaagagagaaacagagagttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1213

Amino Acids

137.7

Weight (kDa)

5.65

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 971
AasI GACNNNNNNGTC 1 cut(s) 3482
Acc36I ACCTGC 2 cut(s) 2854, 2871
AccB1I GGYRCC 2 cut(s) 307, 2677
AccB7I CCANNNNNTGG 3 cut(s) 274, 304, 3566
AccI GTMKAC 2 cut(s) 1878, 2935
AccIII TCCGGA 3 cut(s) 23, 2467, 2819
AciI CCGC 6 cut(s) 292, 447, 657, 1498, 3473, 3528
AclWI GGATC 2 cut(s) 668, 1511
AcoI YGGCCR 1 cut(s) 2693
AcsI RAATTY 6 cut(s) 801, 876, 2111, 2265, 2554, 3403
AcuI CTGAAG 3 cut(s) 1608, 2030, 2126
AcyI GRCGYC 1 cut(s) 2678
AdeI CACNNNGTG 2 cut(s) 249, 3525
AfaI GTAC 5 cut(s) 1635, 1761, 2026, 2999, 3295
AfiI CCNNNNNNNGG 9 cut(s) 274, 304, 947, 1394, 2593, 3376, 3377, 3459, 3566
AflIII ACRYGT 4 cut(s) 74, 1258, 1636, 1881
AjiI CACGTC 2 cut(s) 1236, 1259
AjnI CCWGG 3 cut(s) 273, 1519, 3121
AjuI GAANNNNNNNTTGG 4 cut(s) 1190, 1222, 2571, 2603
AleI CACNNNNGTG 1 cut(s) 79
Alw21I GWGCWC 1 cut(s) 2522
Alw26I GTCTC 8 cut(s) 38, 1242, 1438, 1663, 2159, 2966, 3120, 3599
AlwI GGATC 2 cut(s) 668, 1511
Ama87I CYCGRG 4 cut(s) 125, 199, 681, 2952
Aor13HI TCCGGA 3 cut(s) 23, 2467, 2819
AoxI GGCC 6 cut(s) 166, 295, 838, 2584, 2693, 3450
ApeKI GCWGC 4 cut(s) 504, 2094, 2212, 2849
ApoI RAATTY 6 cut(s) 801, 876, 2111, 2265, 2554, 3403
ArsI GACNNNNNNTTYG 2 cut(s) 1248, 1280
Asp700I GAANNNNTTC 5 cut(s) 533, 577, 789, 1554, 2391
AspLEI GCGC 3 cut(s) 178, 2680, 3233
AspS9I GGNCC 5 cut(s) 265, 295, 910, 2083, 3571
AsuC2I CCSGG 2 cut(s) 2953, 2954
AsuHPI GGTGA 5 cut(s) 1457, 2036, 2725, 3055, 3263
AsuII TTCGAA 1 cut(s) 1793
AvaI CYCGRG 4 cut(s) 125, 199, 681, 2952
AvaII GGWCC 4 cut(s) 265, 910, 2083, 3571
BaeGI GKGCMC 1 cut(s) 312
BalI TGGCCA 1 cut(s) 2695
BanI GGYRCC 2 cut(s) 307, 2677
BanII GRGCYC 1 cut(s) 132
BauI CACGAG 1 cut(s) 721
BbsI GAAGAC 6 cut(s) 75, 522, 1130, 2456, 2586, 3159
Bbv12I GWGCWC 1 cut(s) 2522
BbvI GCAGC 4 cut(s) 516, 2081, 2199, 2861
BceAI ACGGC 5 cut(s) 181, 1596, 2599, 2691, 3166
BciT130I CCWGG 3 cut(s) 275, 1521, 3123
BcnI CCSGG 2 cut(s) 2953, 2954
BcoDI GTCTC 8 cut(s) 38, 1242, 1438, 1663, 2159, 2966, 3120, 3599
BfaI CTAG 7 cut(s) 104, 978, 1313, 1454, 1610, 2426, 2718
BfmI CTRYAG 2 cut(s) 2213, 2847
BfoI RGCGCY 2 cut(s) 179, 2681
BfuAI ACCTGC 2 cut(s) 2854, 2871
BglII AGATCT 1 cut(s) 1418
BisI GCNGC 5 cut(s) 447, 505, 2095, 2213, 2850
BlsI GCNGC 5 cut(s) 448, 506, 2096, 2214, 2851
Bme1390I CCNGG 5 cut(s) 275, 1521, 2953, 2954, 3123
Bme18I GGWCC 4 cut(s) 265, 910, 2083, 3571
BmeT110I CYCGRG 4 cut(s) 125, 199, 681, 2952
BmgBI CACGTC 2 cut(s) 1236, 1259
BmgT120I GGNCC 5 cut(s) 265, 295, 910, 2083, 3571
BmiI GGNNCC 6 cut(s) 296, 309, 2252, 2679, 2705, 3572
BmrFI CCNGG 5 cut(s) 275, 1521, 2953, 2954, 3123
BmrI ACTGGG 3 cut(s) 623, 1223, 2523
BmsI GCATC 3 cut(s) 1090, 1449, 1814
BmuI ACTGGG 3 cut(s) 623, 1223, 2523
BpiI GAAGAC 6 cut(s) 75, 522, 1130, 2456, 2586, 3159
BplI GAGNNNNNCTC 2 cut(s) 18, 50
BpmI CTGGAG 5 cut(s) 296, 1667, 2057, 2269, 2615
Bpu14I TTCGAA 1 cut(s) 1793
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 2 cut(s) 2953, 2954
Bsa29I ATCGAT 1 cut(s) 918
BsaAI YACGTR 2 cut(s) 77, 1639
BsaHI GRCGYC 1 cut(s) 2678
BsaJI CCNNGG 3 cut(s) 2587, 2952, 3447
BsaWI WCCGGW 4 cut(s) 23, 1189, 2467, 2819
Bsc4I CCNNNNNNNGG 9 cut(s) 274, 304, 947, 1394, 2593, 3376, 3377, 3459, 3566
Bse1I ACTGG 7 cut(s) 267, 432, 618, 1229, 2040, 2518, 3364
Bse3DI GCAATG 2 cut(s) 2859, 2946
BseAI TCCGGA 3 cut(s) 23, 2467, 2819
BseBI CCWGG 3 cut(s) 275, 1521, 3123
BseCI ATCGAT 1 cut(s) 918
BseDI CCNNGG 3 cut(s) 2587, 2952, 3447
BseLI CCNNNNNNNGG 9 cut(s) 274, 304, 947, 1394, 2593, 3376, 3377, 3459, 3566
BseMI GCAATG 2 cut(s) 2859, 2946
BseMII CTCAG 6 cut(s) 1101, 1977, 2532, 2879, 2994, 3363
BseNI ACTGG 7 cut(s) 267, 432, 618, 1229, 2040, 2518, 3364
BseRI GAGGAG 2 cut(s) 696, 1681
BseSI GKGCMC 1 cut(s) 312
BseXI GCAGC 4 cut(s) 516, 2081, 2199, 2861
BseYI CCCAGC 1 cut(s) 3391
BsgI GTGCAG 3 cut(s) 1137, 2080, 2431
BshFI GGCC 6 cut(s) 168, 297, 840, 2586, 2695, 3452
BshNI GGYRCC 2 cut(s) 307, 2677
BshVI ATCGAT 1 cut(s) 918
BsiHKAI GWGCWC 1 cut(s) 2522
BsiHKCI CYCGRG 4 cut(s) 125, 199, 681, 2952
BsiSI CCGG 5 cut(s) 24, 1190, 2468, 2820, 2953
BsiWI CGTACG 1 cut(s) 2997
BslFI GGGAC 2 cut(s) 1556, 3584
BslI CCNNNNNNNGG 9 cut(s) 274, 304, 947, 1394, 2593, 3376, 3377, 3459, 3566
BsmAI GTCTC 8 cut(s) 38, 1242, 1438, 1663, 2159, 2966, 3120, 3599
BsmBI CGTCTC 1 cut(s) 1242
BsmFI GGGAC 2 cut(s) 1556, 3584
BsmI GAATGC 2 cut(s) 1530, 3110
BsnI GGCC 6 cut(s) 168, 297, 840, 2586, 2695, 3452
BsoBI CYCGRG 4 cut(s) 125, 199, 681, 2952
Bsp119I TTCGAA 1 cut(s) 1793
Bsp1286I GDGCHC 3 cut(s) 132, 312, 2522
Bsp13I TCCGGA 3 cut(s) 23, 2467, 2819
Bsp1407I TGTACA 2 cut(s) 1759, 2024
Bsp143I GATC 6 cut(s) 399, 660, 1418, 1516, 2200, 2737
BspACI CCGC 6 cut(s) 292, 447, 657, 1498, 3473, 3528
BspANI GGCC 6 cut(s) 168, 297, 840, 2586, 2695, 3452
BspCNI CTCAG 6 cut(s) 1100, 1978, 2533, 2880, 2995, 3364
BspDI ATCGAT 1 cut(s) 918
BspEI TCCGGA 3 cut(s) 23, 2467, 2819
BspHI TCATGA 1 cut(s) 1366
BspLI GGNNCC 6 cut(s) 296, 309, 2252, 2679, 2705, 3572
BspMAI CTGCAG 2 cut(s) 2217, 2851
BspMI ACCTGC 2 cut(s) 2854, 2871
BspPI GGATC 2 cut(s) 668, 1511
BspQI GCTCTTC 1 cut(s) 2067
BspT104I TTCGAA 1 cut(s) 1793
BspT107I GGYRCC 2 cut(s) 307, 2677
BsrDI GCAATG 2 cut(s) 2859, 2946
BsrGI TGTACA 2 cut(s) 1759, 2024
BsrI ACTGG 7 cut(s) 267, 432, 618, 1229, 2040, 2518, 3364
BssECI CCNNGG 3 cut(s) 2587, 2952, 3447
BssMI GATC 6 cut(s) 399, 660, 1418, 1516, 2200, 2737
BssNAI GTATAC 1 cut(s) 2936
BssNI GRCGYC 1 cut(s) 2678
BssSI CACGAG 1 cut(s) 721
BssT1I CCWWGG 1 cut(s) 2587
Bst1107I GTATAC 1 cut(s) 2936
Bst2BI CACGAG 1 cut(s) 721
Bst2UI CCWGG 3 cut(s) 275, 1521, 3123
Bst4CI ACNGT 5 cut(s) 1405, 1432, 2977, 3178, 3287
Bst6I CTCTTC 5 cut(s) 244, 405, 2067, 3123, 3611
BstACI GRCGYC 1 cut(s) 2678
BstAUI TGTACA 2 cut(s) 1759, 2024
BstBAI YACGTR 2 cut(s) 77, 1639
BstBI TTCGAA 1 cut(s) 1793
BstC8I GCNNGC 9 cut(s) 494, 1061, 1065, 1116, 1273, 2491, 3201, 3245, 3365
BstH2I RGCGCY 2 cut(s) 179, 2681
BstHHI GCGC 3 cut(s) 178, 2680, 3233
BstKTI GATC 6 cut(s) 402, 663, 1421, 1519, 2203, 2740
BstMAI GTCTC 8 cut(s) 38, 1242, 1438, 1663, 2159, 2966, 3120, 3599
BstMBI GATC 6 cut(s) 399, 660, 1418, 1516, 2200, 2737
BstMWI GCNNNNNNNGC 5 cut(s) 663, 1589, 1684, 2359, 3230
BstNI CCWGG 3 cut(s) 275, 1521, 3123
BstNSI RCATGY 4 cut(s) 1067, 1326, 1885, 2493
BstSCI CCNGG 5 cut(s) 273, 1519, 2951, 2952, 3121
BstSFI CTRYAG 2 cut(s) 2213, 2847
BstSLI GKGCMC 1 cut(s) 312
BstV1I GCAGC 4 cut(s) 516, 2081, 2199, 2861
BstV2I GAAGAC 6 cut(s) 75, 522, 1130, 2456, 2586, 3159
BstX2I RGATCY 2 cut(s) 1418, 1516
BstXI CCANNNNNNTGG 2 cut(s) 3158, 3195
BstYI RGATCY 2 cut(s) 1418, 1516
BstZ17I GTATAC 1 cut(s) 2936
Bsu15I ATCGAT 1 cut(s) 918
BsuRI GGCC 6 cut(s) 168, 297, 840, 2586, 2695, 3452
BsuTUI ATCGAT 1 cut(s) 918
BtgZI GCGATG 1 cut(s) 661
BtrI CACGTC 2 cut(s) 1236, 1259
BtsIMutI CAGTG 1 cut(s) 1983
BveI ACCTGC 2 cut(s) 2854, 2871
Cac8I GCNNGC 9 cut(s) 494, 1061, 1065, 1116, 1273, 2491, 3201, 3245, 3365
CciI TCATGA 1 cut(s) 1366
CfoI GCGC 3 cut(s) 178, 2680, 3233
Cfr13I GGNCC 5 cut(s) 265, 295, 910, 2083, 3571
Cfr9I CCCGGG 1 cut(s) 2952
ClaI ATCGAT 1 cut(s) 918
CseI GACGC 1 cut(s) 831
Csp6I GTAC 5 cut(s) 1634, 1760, 2025, 2998, 3294
CviQI GTAC 5 cut(s) 1634, 1760, 2025, 2998, 3294
DinI GGCGCC 1 cut(s) 2679
DpnI GATC 6 cut(s) 401, 662, 1420, 1518, 2202, 2739
DpnII GATC 6 cut(s) 399, 660, 1418, 1516, 2200, 2737
DraI TTTAAA 1 cut(s) 438
DraIII CACNNNGTG 2 cut(s) 249, 3525
DrdI GACNNNNNNGTC 1 cut(s) 3482
DseDI GACNNNNNNGTC 1 cut(s) 3482
EaeI YGGCCR 1 cut(s) 2693
Eam1104I CTCTTC 5 cut(s) 244, 405, 2067, 3123, 3611
EarI CTCTTC 5 cut(s) 244, 405, 2067, 3123, 3611
EciI GGCGGA 2 cut(s) 1487, 3488
Eco130I CCWWGG 1 cut(s) 2587
Eco147I AGGCCT 1 cut(s) 840
Eco24I GRGCYC 1 cut(s) 132
Eco32I GATATC 1 cut(s) 2944
Eco47I GGWCC 4 cut(s) 265, 910, 2083, 3571
Eco57I CTGAAG 3 cut(s) 1608, 2030, 2126
Eco88I CYCGRG 4 cut(s) 125, 199, 681, 2952
EcoO109I RGGNCCY 1 cut(s) 3571
EcoRI GAATTC 3 cut(s) 801, 876, 2265
EcoRII CCWGG 3 cut(s) 273, 1519, 3121
EcoRV GATATC 1 cut(s) 2944
EcoT14I CCWWGG 1 cut(s) 2587
EcoT22I ATGCAT 1 cut(s) 1464
EcoT38I GRGCYC 1 cut(s) 132
EgeI GGCGCC 1 cut(s) 2679
EheI GGCGCC 1 cut(s) 2679
ErhI CCWWGG 1 cut(s) 2587
Esp3I CGTCTC 1 cut(s) 1242
FalI AAGNNNNNCTT 4 cut(s) 1029, 1061, 2571, 2603
FaqI GGGAC 2 cut(s) 1556, 3584
FauI CCCGC 3 cut(s) 285, 650, 3521
FauNDI CATATG 1 cut(s) 3384
FblI GTMKAC 2 cut(s) 1878, 2935
Fnu4HI GCNGC 5 cut(s) 447, 505, 2095, 2213, 2850
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 5 cut(s) 447, 505, 2095, 2213, 2850
FspBI CTAG 7 cut(s) 104, 978, 1313, 1454, 1610, 2426, 2718
GlaI GCGC 3 cut(s) 177, 2679, 3232
GluI GCNGC 5 cut(s) 447, 505, 2095, 2213, 2850
GsaI CCCAGC 1 cut(s) 3395
GsuI CTGGAG 5 cut(s) 296, 1667, 2057, 2269, 2615
HaeII RGCGCY 2 cut(s) 179, 2681
HaeIII GGCC 6 cut(s) 168, 297, 840, 2586, 2695, 3452
HapII CCGG 5 cut(s) 24, 1190, 2468, 2820, 2953
HgaI GACGC 1 cut(s) 831
HhaI GCGC 3 cut(s) 178, 2680, 3233
Hin1I GRCGYC 1 cut(s) 2678
Hin6I GCGC 3 cut(s) 176, 2678, 3231
HinP1I GCGC 3 cut(s) 176, 2678, 3231
HincII GTYRAC 3 cut(s) 672, 1029, 1879
HindII GTYRAC 3 cut(s) 672, 1029, 1879
HindIII AAGCTT 3 cut(s) 358, 369, 2012
HpaII CCGG 5 cut(s) 24, 1190, 2468, 2820, 2953
HphI GGTGA 5 cut(s) 1457, 2036, 2725, 3055, 3263
Hpy99I CGWCG 4 cut(s) 47, 167, 825, 1880
HpyAV CCTTC 7 cut(s) 142, 736, 1592, 2264, 2504, 2566, 3025
HpyCH4III ACNGT 5 cut(s) 1405, 1432, 2977, 3178, 3287
HpyCH4IV ACGT 7 cut(s) 45, 76, 1235, 1258, 1638, 1875, 3079
HpyF10VI GCNNNNNNNGC 5 cut(s) 663, 1589, 1684, 2359, 3230
HpySE526I ACGT 7 cut(s) 45, 76, 1235, 1258, 1638, 1875, 3079
Hsp92I GRCGYC 1 cut(s) 2678
HspAI GCGC 3 cut(s) 176, 2678, 3231
KasI GGCGCC 1 cut(s) 2677
Kpn2I TCCGGA 3 cut(s) 23, 2467, 2819
Kzo9I GATC 6 cut(s) 399, 660, 1418, 1516, 2200, 2737
LguI GCTCTTC 1 cut(s) 2067
LmnI GCTCC 5 cut(s) 709, 957, 1163, 2250, 2359
Lsp1109I GCAGC 4 cut(s) 516, 2081, 2199, 2861
LweI GCATC 3 cut(s) 1090, 1449, 1814
MaeI CTAG 7 cut(s) 104, 978, 1313, 1454, 1610, 2426, 2718
MaeII ACGT 7 cut(s) 45, 76, 1235, 1258, 1638, 1875, 3079
MaeIII GTNAC 9 cut(s) 1094, 1231, 1354, 1426, 2720, 2750, 3172, 3183, 3517
MalI GATC 6 cut(s) 401, 662, 1420, 1518, 2202, 2739
MboI GATC 6 cut(s) 399, 660, 1418, 1516, 2200, 2737
MfeI CAATTG 3 cut(s) 2193, 2382, 3193
MflI RGATCY 2 cut(s) 1418, 1516
MhlI GDGCHC 3 cut(s) 132, 312, 2522
MlsI TGGCCA 1 cut(s) 2695
MluNI TGGCCA 1 cut(s) 2695
Mly113I GGCGCC 1 cut(s) 2678
MlyI GAGTC 2 cut(s) 191, 2911
MmeI TCCRAC 6 cut(s) 396, 550, 619, 1188, 1911, 2061
Mox20I TGGCCA 1 cut(s) 2695
Mph1103I ATGCAT 1 cut(s) 1464
MroI TCCGGA 3 cut(s) 23, 2467, 2819
MroXI GAANNNNTTC 5 cut(s) 533, 577, 789, 1554, 2391
MscI TGGCCA 1 cut(s) 2695
MslI CAYNNNNRTG 6 cut(s) 79, 524, 1293, 1641, 2650, 3156
Msp20I TGGCCA 1 cut(s) 2695
MspI CCGG 5 cut(s) 24, 1190, 2468, 2820, 2953
MspR9I CCNGG 5 cut(s) 275, 1521, 2953, 2954, 3123
MunI CAATTG 3 cut(s) 2193, 2382, 3193
Mva1269I GAATGC 2 cut(s) 1530, 3110
MvaI CCWGG 3 cut(s) 275, 1521, 3123
MwoI GCNNNNNNNGC 5 cut(s) 663, 1589, 1684, 2359, 3230
NarI GGCGCC 1 cut(s) 2678
NciI CCSGG 2 cut(s) 2953, 2954
NdeI CATATG 1 cut(s) 3384
NdeII GATC 6 cut(s) 399, 660, 1418, 1516, 2200, 2737
NlaIV GGNNCC 6 cut(s) 296, 309, 2252, 2679, 2705, 3572
NmuCI GTSAC 3 cut(s) 1231, 2750, 3517
NsiI ATGCAT 1 cut(s) 1464
NspI RCATGY 4 cut(s) 1067, 1326, 1885, 2493
NspV TTCGAA 1 cut(s) 1793
OliI CACNNNNGTG 1 cut(s) 79
PaeI GCATGC 2 cut(s) 1067, 2493
PaeR7I CTCGAG 3 cut(s) 125, 199, 681
PagI TCATGA 1 cut(s) 1366
PceI AGGCCT 1 cut(s) 840
PciI ACATGT 1 cut(s) 1881
PciSI GCTCTTC 1 cut(s) 2067
PcsI WCGNNNNNNNCGW 2 cut(s) 206, 2315
PctI GAATGC 2 cut(s) 1530, 3110
PdmI GAANNNNTTC 5 cut(s) 533, 577, 789, 1554, 2391
PfeI GAWTC 9 cut(s) 140, 578, 626, 642, 1363, 1505, 2642, 3275, 3553
Pfl23II CGTACG 1 cut(s) 2997
PflMI CCANNNNNTGG 3 cut(s) 274, 304, 3566
PfoI TCCNGGA 3 cut(s) 273, 1519, 3121
PkrI GCNGC 5 cut(s) 448, 506, 2096, 2214, 2851
PleI GAGTC 2 cut(s) 191, 2910
PluTI GGCGCC 1 cut(s) 2681
PpsI GAGTC 2 cut(s) 191, 2910
Ppu21I YACGTR 2 cut(s) 77, 1639
PpuMI RGGWCCY 1 cut(s) 3571
PscI ACATGT 1 cut(s) 1881
PsiI TTATAA 1 cut(s) 971
Psp5II RGGWCCY 1 cut(s) 3571
Psp6I CCWGG 3 cut(s) 273, 1519, 3121
PspFI CCCAGC 1 cut(s) 3391
PspGI CCWGG 3 cut(s) 273, 1519, 3121
PspLI CGTACG 1 cut(s) 2997
PspN4I GGNNCC 6 cut(s) 296, 309, 2252, 2679, 2705, 3572
PspPI GGNCC 5 cut(s) 265, 295, 910, 2083, 3571
PspPPI RGGWCCY 1 cut(s) 3571
PspXI VCTCGAGB 3 cut(s) 125, 199, 681
PsrI GAACNNNNNNTAC 2 cut(s) 1743, 1775
PstI CTGCAG 2 cut(s) 2217, 2851
PsuI RGATCY 2 cut(s) 1418, 1516
RsaI GTAC 5 cut(s) 1635, 1761, 2026, 2999, 3295
RsaNI GTAC 5 cut(s) 1634, 1760, 2025, 2998, 3294
RseI CAYNNNNRTG 6 cut(s) 79, 524, 1293, 1641, 2650, 3156
SalI GTCGAC 1 cut(s) 1877
SapI GCTCTTC 1 cut(s) 2067
SatI GCNGC 5 cut(s) 447, 505, 2095, 2213, 2850
Sau3AI GATC 6 cut(s) 399, 660, 1418, 1516, 2200, 2737
Sau96I GGNCC 5 cut(s) 265, 295, 910, 2083, 3571
SchI GAGTC 2 cut(s) 191, 2911
ScrFI CCNGG 5 cut(s) 275, 1521, 2953, 2954, 3123
SduI GDGCHC 3 cut(s) 132, 312, 2522
SfaNI GCATC 3 cut(s) 1090, 1449, 1814
SfcI CTRYAG 2 cut(s) 2213, 2847
SfoI GGCGCC 1 cut(s) 2679
Sfr274I CTCGAG 3 cut(s) 125, 199, 681
SfuI TTCGAA 1 cut(s) 1793
SinI GGWCC 4 cut(s) 265, 910, 2083, 3571
SlaI CTCGAG 3 cut(s) 125, 199, 681
SmaI CCCGGG 1 cut(s) 2954
SmiMI CAYNNNNRTG 6 cut(s) 79, 524, 1293, 1641, 2650, 3156
SmlI CTYRAG 4 cut(s) 125, 199, 350, 681
SmoI CTYRAG 4 cut(s) 125, 199, 350, 681
SphI GCATGC 2 cut(s) 1067, 2493
SseBI AGGCCT 1 cut(s) 840
SsiI CCGC 6 cut(s) 292, 447, 657, 1498, 3473, 3528
SspDI GGCGCC 1 cut(s) 2677
SspMI CTAG 7 cut(s) 104, 978, 1313, 1454, 1610, 2426, 2718
StuI AGGCCT 1 cut(s) 840
StyD4I CCNGG 5 cut(s) 273, 1519, 2951, 2952, 3121
StyI CCWWGG 1 cut(s) 2587
TaaI ACNGT 5 cut(s) 1405, 1432, 2977, 3178, 3287
TaiI ACGT 7 cut(s) 48, 79, 1238, 1261, 1641, 1878, 3082
TatI WGTACW 2 cut(s) 1759, 2024
TauI GCSGC 1 cut(s) 449
TfiI GAWTC 9 cut(s) 140, 578, 626, 642, 1363, 1505, 2642, 3275, 3553
TscAI CASTG 1 cut(s) 1990
TseFI GTSAC 3 cut(s) 1231, 2750, 3517
TseI GCWGC 4 cut(s) 504, 2094, 2212, 2849
Tsp45I GTSAC 3 cut(s) 1231, 2750, 3517
TspGWI ACGGA 3 cut(s) 1724, 2985, 3160
TspMI CCCGGG 1 cut(s) 2952
TspRI CASTG 1 cut(s) 1990
Van91I CCANNNNNTGG 3 cut(s) 274, 304, 3566
VpaK11BI GGWCC 4 cut(s) 265, 910, 2083, 3571
XapI RAATTY 6 cut(s) 801, 876, 2111, 2265, 2554, 3403
XbaI TCTAGA 1 cut(s) 1312
XceI RCATGY 4 cut(s) 1067, 1326, 1885, 2493
XcmI CCANNNNNNNNNTGG 1 cut(s) 1927
XhoI CTCGAG 3 cut(s) 125, 199, 681
XmaI CCCGGG 1 cut(s) 2952
XmiI GTMKAC 2 cut(s) 1878, 2935
XmnI GAANNNNTTC 5 cut(s) 533, 577, 789, 1554, 2391
XspI CTAG 7 cut(s) 104, 978, 1313, 1454, 1610, 2426, 2718
Zsp2I ATGCAT 1 cut(s) 1464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.