MD07G1243500.v1.1

RESISTANCE protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000067
Physical Location & Seq
Reverse (-)
31375560 .. 31381258
5699 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1243500.v1.1.491

Sequence Viewer

Length: 2736 bp
ATGGAAGAGGTTCAGAGCTGGAGATCCGCTCTTACAACTGCCACCAGTTTATCCGGCTGGGATTCGCGAAACTATGAGAATGATGTGGTGCTTATTGAGGAAATTGTAGAAGATGTTTATAGGAAATTGATCGACATCTCATCAACATCAAGCAAAGATAATGGCTTGGTTGACATGGATTCTCACATGCATGAAATGCATCTATTATTATATCCTCCCGGAGGTGAAACGAATACTGTTCGCATTGTTGGAATATGGGGTATGGGTGGTTTAGGCAAAACAACCATCGCTAGAGCTGTTCATGATAAAATCGCTAGTCGATTTGAAGCTTGTTGCTTTCTTGAAAATTTCAAGGAAGGTTTCATGAAGCATGGCAAACTACATATGCAGGCACAACTTCTATTGAGTATCTCGAACAACAAGGTGGGGAGTTCTGACATATCGAGTAAAGGTTTTCAGGTGATGTTAGAAAGCCTTGGTCAGAGAAAAGTTCTTATTGTTGTTGATGATGTGGACGAATTAGAACAAATTGAAGCTTTACTTGAAGAGCCACATTCCTTTGGTGGTGGAAGCAGAATTATTATAACAACTAGAGATTCGCAATTACTAAGCATAGCTGATGTGATATATAATCCCAAGATTTTGAGTGATTCTGGAGCTCTGAAACTCTTTAGGCGGCACGCCTTCAGAAAAAACCAACCCACCAGAGATTATGATGATCTCTCGAATCATGTCGTAGAATATGCTCAAGGCCTGCCTTTAGCACTCAAAGTCTTGGGAGCTTTTCTTGGTAACAAAACTATACGCGAGTGGGAAGATGAGTTAGAAAAAATAAGGAAAATCCCGCGAAGGGGAATTCATGATGTGCTTAAATCAAGCTTCGATGGACTAGATGACAAGGAGAAGGACATCTTTCTAGACATTGCATGTTTCTTTAAAGGGATGGAGAAAGACTGTGCAGCCCGAATTCTGGAAGATTGTGGTTTCCATCCTTATACTGGAATAAGAGTTCTAATCGATCGAGCACTCATAACTGTCTCAGGGGAGGGGGAACTGGAGATGCATGATTCATTAGCGGAAATGGGTCGGGAAATCGTCCGCCAAGAATGTATCAAAGAGCCTGGGAGACGAAGTAGGTTGTGGAGTTATGAAGATGTTCATCACGTGCTATCTCAAAATACGGCTACAAAAACAGTTGAAAGCATAATTGTGGATTTCTCGTACTCAGGCTGGCCATGCTTAAATGCTGAAGCTTTTGTTAGAATGACTCAACTAAGACTTCTCAAGATCAGTCATAGGCACTTAAATGCTGAATCTGTTGTTAGAAGGATTCAACGAAGACTTCTCACGATCGGTCTTCATGGGAACTTAAATGCTCAACGAAGACTTCTCGAGATCAGTTATAGGCACTTCGTTTTTAAAGATGAGTACTGCAAACAACACCTGTTTGGGTCCTTAAAGTTGCTTAACCTGAGGTATCTCTCCTGGCTTGGTTTCCCTCTCAAGTCTTTGCCATCCAACTTTCAATTCGAAAATCTTGTTGAACTTGACATGCAATGTAGTCTCATTGACCGACTTTGGGAAGGAACCCAGACGCAGGAAAAGTTGAAATTCATCAATTTAAGTTATTGTCAATCCCTTAAGGAAACCCCTGACTTCACAAATGTGCCAAATCTTGAGAGGCTAATTGTTCGAAGTTGTATAAGCTTAGTTGAGGTTCACCCGTCTATTTCGACTCTTACAAACCTTGTTTTATTGGATCTGCATGGGTGCAATGAACTTAAGATTCTACCTAGCAAAATTTATTGCAAGGAACTTAAGATTCTACCTAGCAAAATTCGTATGAGATCTCTCAAAACCTTTAATCTTTCTGACTGCACCAGTCTTGAGATGTTTCCAGAGATTTCAGAAGGTATGGAGGAGTTAGAAGAGCTTAATTTATCCGGGTCAAAAATTAAAGAACTGTCCCTGTCAATTAATAATCTCACGGGGTTGAGTCATTTCAACCTAAAAGATTGCAAGGAACTTAAGAGTCTTCCAAGCTGCATTCATATGAAATGTCTGAAAACCTTTAATCTTTATGGCTGCTTCAGTCTTGAGATGTTTCCATCGATTTCAGAAGGTATTGAGGGGTTAGAAAAGCTTGATTTACCCTGCTCAAAAATTAAAGAACTGTCCTCGTCAATTAATAATCTTACGAGGTTGTGTCATTTGAATCTACATAATTGCACGGAACTTAAGAGTCTTCCAAGCAGCATTCGTATGAAATCTCTCAAAACCTTTAATCTTTCTGGCTGCTCTAGTCTTGAGATGTTTCCAAAGATTTCAGAAGGTATGGAGGAGTTAGAAGAGCTTAATTTATCCGGGTCAAAAATTAAAGAACTGCCCTCGTCAATTAATAATCTTACGGGATTGAATCATTTGAATCTAAAAGATTGCAAAGAACTTAAGAGTCTTCCAAGCAGCATTTGTCAGCTCAAGTCCCTTTTCTCTCTCTCTCTTTCCGGTTGTACAAAATTTGAGTTGTTTCCAAGCATTGAAGAAAATATGGAAGGATTAAGAAGTCTTTTCCTGGATGGAACATCTATTAAAGAGCTTTCCCCCTGGATTGAACGCCTTACGGAGCTTCGGTATTTATATTTGAGAGACTGCAAAAGCCTTGTACATCGTCCCAACACGCTCTGTAATTTGTATTTTCATAGCCCTCTCGAGGACGCATATCGCCGTCTTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_3 LRR_5 LRR_8 NB-ARC TIR

Protein Analysis

912

Amino Acids

103.71

Weight (kDa)

6.31

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 78 - 232 4.8e-16 NB-ARC domain
WHD_ROQ1 PF23282 299 - 368 4.9e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 616 - 696 6.4e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 687 - 769 5.7e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_EndoS PF23952 714 - 796 3.9e-06 Endo-beta-N-acetylglucosaminidase EndoS LRR domain
LRR_13 PF23286 756 - 839 2.4e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 784 - 881 1.5e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 584
AccBSI CCGCTC 1 cut(s) 29
AccII CGCG 3 cut(s) 67, 807, 847
AciI CCGC 5 cut(s) 27, 676, 845, 1076, 1099
AclWI GGATC 2 cut(s) 18, 1767
AcoI YGGCCR 1 cut(s) 1232
AcsI RAATTY 7 cut(s) 346, 855, 966, 1610, 1800, 1836, 2516
AcuI CTGAAG 3 cut(s) 670, 1269, 2074
AcvI CACGTG 1 cut(s) 1165
AfaI GTAC 4 cut(s) 1223, 1430, 2512, 2664
AfiI CCNNNNNNNGG 8 cut(s) 221, 850, 851, 970, 998, 1579, 1597, 2710
AflII CTTAAG 6 cut(s) 1640, 1781, 1817, 2027, 2237, 2447
AjnI CCWGG 4 cut(s) 1120, 1484, 2571, 2603
AjuI GAANNNNNNNTTGG 2 cut(s) 1511, 1543
AleI CACNNNNGTG 1 cut(s) 1664
AloI GAACNNNNNNTCC 2 cut(s) 993, 1025
Alw21I GWGCWC 2 cut(s) 661, 1027
Alw26I GTCTC 4 cut(s) 1042, 1120, 1568, 2640
AlwI GGATC 2 cut(s) 18, 1767
Ama87I CYCGRG 2 cut(s) 1391, 2708
AoxI GGCC 2 cut(s) 751, 1232
ApeKI GCWGC 6 cut(s) 959, 2043, 2085, 2253, 2295, 2463
ApoI RAATTY 7 cut(s) 346, 855, 966, 1610, 1800, 1836, 2516
AseI ATTAAT 3 cut(s) 1977, 2187, 2397
Asp700I GAANNNNTTC 2 cut(s) 9, 1155
AspS9I GGNCC 1 cut(s) 1452
AsuC2I CCSGG 3 cut(s) 219, 1945, 2365
AsuHPI GGTGA 3 cut(s) 236, 472, 1712
AsuII TTCGAA 2 cut(s) 1530, 1693
AvaI CYCGRG 2 cut(s) 1391, 2708
AvaII GGWCC 1 cut(s) 1452
AxyI CCTNAGG 1 cut(s) 1472
BalI TGGCCA 1 cut(s) 1234
BanII GRGCYC 1 cut(s) 661
BbrPI CACGTG 1 cut(s) 1165
BbsI GAAGAC 6 cut(s) 1345, 1349, 1390, 2027, 2237, 2447
Bbv12I GWGCWC 2 cut(s) 661, 1027
BbvI GCAGC 6 cut(s) 971, 2030, 2072, 2265, 2282, 2475
BccI CCATC 7 cut(s) 293, 878, 937, 996, 1522, 2116, 2570
BceAI ACGGC 2 cut(s) 1197, 2709
BciT130I CCWGG 4 cut(s) 1122, 1486, 2573, 2605
BcnI CCSGG 3 cut(s) 219, 1945, 2365
BcoDI GTCTC 4 cut(s) 1042, 1120, 1568, 2640
BfaI CTAG 8 cut(s) 291, 315, 591, 890, 917, 1794, 1830, 2301
BfrI CTTAAG 6 cut(s) 1640, 1781, 1817, 2027, 2237, 2447
BglI GCCNNNNNGGC 1 cut(s) 2730
BglII AGATCT 1 cut(s) 1847
BisI GCNGC 7 cut(s) 677, 960, 2044, 2086, 2254, 2296, 2464
BlsI GCNGC 7 cut(s) 678, 961, 2045, 2087, 2255, 2297, 2465
BmcAI AGTACT 1 cut(s) 1430
Bme1390I CCNGG 7 cut(s) 219, 1122, 1486, 1945, 2365, 2573, 2605
Bme18I GGWCC 1 cut(s) 1452
BmeT110I CYCGRG 2 cut(s) 1391, 2708
BmgT120I GGNCC 1 cut(s) 1452
BmiI GGNNCC 2 cut(s) 1453, 1588
BmrFI CCNGG 7 cut(s) 219, 1122, 1486, 1945, 2365, 2573, 2605
BmsI GCATC 2 cut(s) 208, 1050
BpiI GAAGAC 6 cut(s) 1345, 1349, 1390, 2027, 2237, 2447
BplI GAGNNNNNCTC 2 cut(s) 13, 45
BpmI CTGGAG 3 cut(s) 40, 675, 1076
Bpu14I TTCGAA 2 cut(s) 1530, 1693
BpuEI CTTGAG 8 cut(s) 732, 1268, 1487, 1697, 1907, 2117, 2327, 2462
BpuMI CCSGG 3 cut(s) 219, 1945, 2365
Bsa29I ATCGAT 2 cut(s) 1017, 2111
BsaAI YACGTR 1 cut(s) 1165
BsaBI GATNNNNATC 2 cut(s) 134, 1158
BsaJI CCNNGG 3 cut(s) 475, 1121, 2603
BsaWI WCCGGW 1 cut(s) 2504
BsaXI ACNNNNNCTCC 4 cut(s) 1117, 1147, 2615, 2645
Bsc4I CCNNNNNNNGG 8 cut(s) 221, 850, 851, 970, 998, 1579, 1597, 2710
Bse1I ACTGG 4 cut(s) 45, 1003, 1059, 1881
Bse21I CCTNAGG 1 cut(s) 1472
Bse3DI GCAATG 3 cut(s) 921, 1562, 1780
Bse8I GATNNNNATC 2 cut(s) 134, 1158
BseBI CCWGG 4 cut(s) 1122, 1486, 2573, 2605
BseCI ATCGAT 2 cut(s) 1017, 2111
BseDI CCNNGG 3 cut(s) 475, 1121, 2603
BseGI GGATG 4 cut(s) 948, 988, 1514, 2581
BseJI GATNNNNATC 2 cut(s) 134, 1158
BseLI CCNNNNNNNGG 8 cut(s) 221, 850, 851, 970, 998, 1579, 1597, 2710
BseMI GCAATG 3 cut(s) 921, 1562, 1780
BseMII CTCAG 3 cut(s) 1053, 1239, 1463
BseNI ACTGG 4 cut(s) 45, 1003, 1059, 1881
BseRI GAGGAG 2 cut(s) 1934, 2354
BseXI GCAGC 6 cut(s) 971, 2030, 2072, 2265, 2282, 2475
BseYI CCCAGC 1 cut(s) 57
BsgI GTGCAG 2 cut(s) 978, 1861
Bsh1236I CGCG 3 cut(s) 67, 807, 847
Bsh1285I CGRYCG 2 cut(s) 1021, 1353
BshFI GGCC 2 cut(s) 753, 1234
BshVI ATCGAT 2 cut(s) 1017, 2111
BsiEI CGRYCG 2 cut(s) 1021, 1353
BsiHKAI GWGCWC 2 cut(s) 661, 1027
BsiHKCI CYCGRG 2 cut(s) 1391, 2708
BsiSI CCGG 5 cut(s) 54, 219, 1944, 2364, 2505
BslFI GGGAC 3 cut(s) 1951, 2467, 2655
BslI CCNNNNNNNGG 8 cut(s) 221, 850, 851, 970, 998, 1579, 1597, 2710
BsmAI GTCTC 4 cut(s) 1042, 1120, 1568, 2640
BsmBI CGTCTC 1 cut(s) 1120
BsmFI GGGAC 3 cut(s) 1951, 2467, 2655
BsmI GAATGC 2 cut(s) 2046, 2256
BsnI GGCC 2 cut(s) 753, 1234
BsoBI CYCGRG 2 cut(s) 1391, 2708
Bsp119I TTCGAA 2 cut(s) 1530, 1693
Bsp1286I GDGCHC 2 cut(s) 661, 1027
Bsp1407I TGTACA 2 cut(s) 2510, 2662
Bsp143I GATC 9 cut(s) 23, 129, 718, 1018, 1287, 1350, 1395, 1759, 1847
Bsp68I TCGCGA 1 cut(s) 67
BspACI CCGC 5 cut(s) 27, 676, 845, 1076, 1099
BspANI GGCC 2 cut(s) 753, 1234
BspCNI CTCAG 3 cut(s) 1052, 1238, 1464
BspDI ATCGAT 2 cut(s) 1017, 2111
BspFNI CGCG 3 cut(s) 67, 807, 847
BspHI TCATGA 3 cut(s) 301, 363, 859
BspLI GGNNCC 2 cut(s) 1453, 1588
BspPI GGATC 2 cut(s) 18, 1767
BspQI GCTCTTC 3 cut(s) 540, 1923, 2343
BspT104I TTCGAA 2 cut(s) 1530, 1693
BspTI CTTAAG 6 cut(s) 1640, 1781, 1817, 2027, 2237, 2447
BsrBI CCGCTC 1 cut(s) 29
BsrDI GCAATG 3 cut(s) 921, 1562, 1780
BsrGI TGTACA 2 cut(s) 2510, 2662
BsrI ACTGG 4 cut(s) 45, 1003, 1059, 1881
BssECI CCNNGG 3 cut(s) 475, 1121, 2603
BssMI GATC 9 cut(s) 23, 129, 718, 1018, 1287, 1350, 1395, 1759, 1847
BssT1I CCWWGG 1 cut(s) 475
Bst2UI CCWGG 4 cut(s) 1122, 1486, 2573, 2605
Bst4CI ACNGT 6 cut(s) 238, 956, 1036, 1195, 1965, 2175
Bst6I CTCTTC 3 cut(s) 540, 1923, 2343
BstAFI CTTAAG 6 cut(s) 1640, 1781, 1817, 2027, 2237, 2447
BstAPI GCANNNNNTGC 1 cut(s) 196
BstAUI TGTACA 2 cut(s) 2510, 2662
BstBAI YACGTR 1 cut(s) 1165
BstBI TTCGAA 2 cut(s) 1530, 1693
BstC8I GCNNGC 4 cut(s) 390, 681, 755, 1232
BstDEI CTNAG 6 cut(s) 608, 1039, 1225, 1274, 1472, 1708
BstENI CCTNNNNNAGG 1 cut(s) 219
BstF5I GGATG 4 cut(s) 948, 988, 1514, 2581
BstFNI CGCG 3 cut(s) 67, 807, 847
BstKTI GATC 9 cut(s) 26, 132, 721, 1021, 1290, 1353, 1398, 1762, 1850
BstMAI GTCTC 4 cut(s) 1042, 1120, 1568, 2640
BstMBI GATC 9 cut(s) 23, 129, 718, 1018, 1287, 1350, 1395, 1759, 1847
BstMCI CGRYCG 2 cut(s) 1021, 1353
BstMWI GCNNNNNNNGC 3 cut(s) 196, 1236, 2730
BstNI CCWGG 4 cut(s) 1122, 1486, 2573, 2605
BstNSI RCATGY 3 cut(s) 190, 930, 1555
BstSCI CCNGG 7 cut(s) 217, 1120, 1484, 1943, 2363, 2571, 2603
BstUI CGCG 3 cut(s) 67, 807, 847
BstV1I GCAGC 6 cut(s) 971, 2030, 2072, 2265, 2282, 2475
BstV2I GAAGAC 6 cut(s) 1345, 1349, 1390, 2027, 2237, 2447
BstX2I RGATCY 3 cut(s) 23, 1759, 1847
BstYI RGATCY 3 cut(s) 23, 1759, 1847
Bsu15I ATCGAT 2 cut(s) 1017, 2111
Bsu36I CCTNAGG 1 cut(s) 1472
BsuRI GGCC 2 cut(s) 753, 1234
BsuTUI ATCGAT 2 cut(s) 1017, 2111
BtgZI GCGATG 1 cut(s) 271
BtsCI GGATG 4 cut(s) 948, 988, 1514, 2581
BtuMI TCGCGA 1 cut(s) 67
Cac8I GCNNGC 4 cut(s) 390, 681, 755, 1232
CciI TCATGA 3 cut(s) 301, 363, 859
Cfr13I GGNCC 1 cut(s) 1452
ClaI ATCGAT 2 cut(s) 1017, 2111
CseI GACGC 2 cut(s) 1603, 2723
Csp6I GTAC 4 cut(s) 1222, 1429, 2511, 2663
CviQI GTAC 4 cut(s) 1222, 1429, 2511, 2663
DdeI CTNAG 6 cut(s) 608, 1039, 1225, 1274, 1472, 1708
DpnI GATC 9 cut(s) 25, 131, 720, 1020, 1289, 1352, 1397, 1761, 1849
DpnII GATC 9 cut(s) 23, 129, 718, 1018, 1287, 1350, 1395, 1759, 1847
DraI TTTAAA 2 cut(s) 937, 1420
EaeI YGGCCR 1 cut(s) 1232
Eam1104I CTCTTC 3 cut(s) 540, 1923, 2343
EarI CTCTTC 3 cut(s) 540, 1923, 2343
EciI GGCGGA 1 cut(s) 1088
Ecl136II GAGCTC 1 cut(s) 659
Eco130I CCWWGG 1 cut(s) 475
Eco147I AGGCCT 1 cut(s) 753
Eco24I GRGCYC 1 cut(s) 661
Eco47I GGWCC 1 cut(s) 1452
Eco53kI GAGCTC 1 cut(s) 659
Eco57I CTGAAG 3 cut(s) 670, 1269, 2074
Eco72I CACGTG 1 cut(s) 1165
Eco81I CCTNAGG 1 cut(s) 1472
Eco88I CYCGRG 2 cut(s) 1391, 2708
EcoICRI GAGCTC 1 cut(s) 659
EcoNI CCTNNNNNAGG 1 cut(s) 219
EcoO109I RGGNCCY 1 cut(s) 1452
EcoRI GAATTC 2 cut(s) 855, 966
EcoRII CCWGG 4 cut(s) 1120, 1484, 2571, 2603
EcoT14I CCWWGG 1 cut(s) 475
EcoT22I ATGCAT 3 cut(s) 192, 201, 1065
EcoT38I GRGCYC 1 cut(s) 661
ErhI CCWWGG 1 cut(s) 475
Esp3I CGTCTC 1 cut(s) 1120
FalI AAGNNNNNCTT 4 cut(s) 525, 557, 896, 928
FaqI GGGAC 3 cut(s) 1951, 2467, 2655
FauI CCCGC 1 cut(s) 852
FauNDI CATATG 2 cut(s) 384, 2052
Fnu4HI GCNGC 7 cut(s) 677, 960, 2044, 2086, 2254, 2296, 2464
FokI GGATG 4 cut(s) 955, 975, 1501, 2588
FriOI GRGCYC 1 cut(s) 661
Fsp4HI GCNGC 7 cut(s) 677, 960, 2044, 2086, 2254, 2296, 2464
FspBI CTAG 8 cut(s) 291, 315, 591, 890, 917, 1794, 1830, 2301
GluI GCNGC 7 cut(s) 677, 960, 2044, 2086, 2254, 2296, 2464
GsaI CCCAGC 1 cut(s) 61
GsuI CTGGAG 3 cut(s) 40, 675, 1076
HaeIII GGCC 2 cut(s) 753, 1234
HapII CCGG 5 cut(s) 54, 219, 1944, 2364, 2505
HgaI GACGC 2 cut(s) 1603, 2723
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 6 cut(s) 327, 534, 877, 1251, 1705, 2141
HpaII CCGG 5 cut(s) 54, 219, 1944, 2364, 2505
HphI GGTGA 3 cut(s) 236, 472, 1712
Hpy166II GTNNAC 3 cut(s) 172, 514, 1720
Hpy8I GTNNAC 3 cut(s) 172, 514, 1720
HpyCH4III ACNGT 6 cut(s) 238, 956, 1036, 1195, 1965, 2175
HpyCH4IV ACGT 1 cut(s) 1164
HpyF10VI GCNNNNNNNGC 3 cut(s) 196, 1236, 2730
HpyF3I CTNAG 6 cut(s) 608, 1039, 1225, 1274, 1472, 1708
HpySE526I ACGT 1 cut(s) 1164
Kzo9I GATC 9 cut(s) 23, 129, 718, 1018, 1287, 1350, 1395, 1759, 1847
LguI GCTCTTC 3 cut(s) 540, 1923, 2343
LmnI GCTCC 3 cut(s) 656, 779, 2623
Lsp1109I GCAGC 6 cut(s) 971, 2030, 2072, 2265, 2282, 2475
LweI GCATC 2 cut(s) 208, 1050
MaeI CTAG 8 cut(s) 291, 315, 591, 890, 917, 1794, 1830, 2301
MaeII ACGT 1 cut(s) 1164
MaeIII GTNAC 1 cut(s) 791
MalI GATC 9 cut(s) 25, 131, 720, 1020, 1289, 1352, 1397, 1761, 1849
MbiI CCGCTC 1 cut(s) 29
MboI GATC 9 cut(s) 23, 129, 718, 1018, 1287, 1350, 1395, 1759, 1847
MflI RGATCY 3 cut(s) 23, 1759, 1847
MhlI GDGCHC 2 cut(s) 661, 1027
MlsI TGGCCA 1 cut(s) 1234
MluNI TGGCCA 1 cut(s) 1234
MlyI GAGTC 6 cut(s) 1261, 1729, 2005, 2041, 2251, 2461
MmeI TCCRAC 2 cut(s) 229, 1542
Mox20I TGGCCA 1 cut(s) 1234
Mph1103I ATGCAT 3 cut(s) 192, 201, 1065
MroXI GAANNNNTTC 2 cut(s) 9, 1155
MscI TGGCCA 1 cut(s) 1234
MslI CAYNNNNRTG 6 cut(s) 189, 1208, 1305, 1664, 2051, 2261
Msp20I TGGCCA 1 cut(s) 1234
MspCI CTTAAG 6 cut(s) 1640, 1781, 1817, 2027, 2237, 2447
MspI CCGG 5 cut(s) 54, 219, 1944, 2364, 2505
MspR9I CCNGG 7 cut(s) 219, 1122, 1486, 1945, 2365, 2573, 2605
Mva1269I GAATGC 2 cut(s) 2046, 2256
MvaI CCWGG 4 cut(s) 1122, 1486, 2573, 2605
MvnI CGCG 3 cut(s) 67, 807, 847
MwoI GCNNNNNNNGC 3 cut(s) 196, 1236, 2730
NciI CCSGG 3 cut(s) 219, 1945, 2365
NdeI CATATG 2 cut(s) 384, 2052
NdeII GATC 9 cut(s) 23, 129, 718, 1018, 1287, 1350, 1395, 1759, 1847
NlaIV GGNNCC 2 cut(s) 1453, 1588
NruI TCGCGA 1 cut(s) 67
NsiI ATGCAT 3 cut(s) 192, 201, 1065
NspI RCATGY 3 cut(s) 190, 930, 1555
NspV TTCGAA 2 cut(s) 1530, 1693
OliI CACNNNNGTG 1 cut(s) 1664
PaeR7I CTCGAG 2 cut(s) 1391, 2708
PagI TCATGA 3 cut(s) 301, 363, 859
PceI AGGCCT 1 cut(s) 753
PciSI GCTCTTC 3 cut(s) 540, 1923, 2343
PctI GAATGC 2 cut(s) 2046, 2256
PdmI GAANNNNTTC 2 cut(s) 9, 1155
PfoI TCCNGGA 2 cut(s) 217, 2571
PkrI GCNGC 7 cut(s) 678, 961, 2045, 2087, 2255, 2297, 2465
Ple19I CGATCG 2 cut(s) 1021, 1353
PleI GAGTC 6 cut(s) 1261, 1729, 2004, 2040, 2250, 2460
PmaCI CACGTG 1 cut(s) 1165
PmlI CACGTG 1 cut(s) 1165
PpsI GAGTC 6 cut(s) 1261, 1729, 2004, 2040, 2250, 2460
Ppu21I YACGTR 1 cut(s) 1165
PpuMI RGGWCCY 1 cut(s) 1452
PshBI ATTAAT 3 cut(s) 1977, 2187, 2397
PsiI TTATAA 1 cut(s) 584
Psp124BI GAGCTC 1 cut(s) 661
Psp5II RGGWCCY 1 cut(s) 1452
Psp6I CCWGG 4 cut(s) 1120, 1484, 2571, 2603
PspCI CACGTG 1 cut(s) 1165
PspFI CCCAGC 1 cut(s) 57
PspGI CCWGG 4 cut(s) 1120, 1484, 2571, 2603
PspN4I GGNNCC 2 cut(s) 1453, 1588
PspPI GGNCC 1 cut(s) 1452
PspPPI RGGWCCY 1 cut(s) 1452
PsuI RGATCY 3 cut(s) 23, 1759, 1847
PvuI CGATCG 2 cut(s) 1021, 1353
RruI TCGCGA 1 cut(s) 67
RsaI GTAC 4 cut(s) 1223, 1430, 2512, 2664
RsaNI GTAC 4 cut(s) 1222, 1429, 2511, 2663
RseI CAYNNNNRTG 6 cut(s) 189, 1208, 1305, 1664, 2051, 2261
SacI GAGCTC 1 cut(s) 661
SapI GCTCTTC 3 cut(s) 540, 1923, 2343
SatI GCNGC 7 cut(s) 677, 960, 2044, 2086, 2254, 2296, 2464
Sau3AI GATC 9 cut(s) 23, 129, 718, 1018, 1287, 1350, 1395, 1759, 1847
Sau96I GGNCC 1 cut(s) 1452
ScaI AGTACT 1 cut(s) 1430
SchI GAGTC 6 cut(s) 1261, 1729, 2005, 2041, 2251, 2461
ScrFI CCNGG 7 cut(s) 219, 1122, 1486, 1945, 2365, 2573, 2605
SduI GDGCHC 2 cut(s) 661, 1027
SfaNI GCATC 2 cut(s) 208, 1050
Sfr274I CTCGAG 2 cut(s) 1391, 2708
SfuI TTCGAA 2 cut(s) 1530, 1693
SinI GGWCC 1 cut(s) 1452
SlaI CTCGAG 2 cut(s) 1391, 2708
SmiMI CAYNNNNRTG 6 cut(s) 189, 1208, 1305, 1664, 2051, 2261
SseBI AGGCCT 1 cut(s) 753
SsiI CCGC 5 cut(s) 27, 676, 845, 1076, 1099
SspMI CTAG 8 cut(s) 291, 315, 591, 890, 917, 1794, 1830, 2301
SstI GAGCTC 1 cut(s) 661
StuI AGGCCT 1 cut(s) 753
StyD4I CCNGG 7 cut(s) 217, 1120, 1484, 1943, 2363, 2571, 2603
StyI CCWWGG 1 cut(s) 475
TaaI ACNGT 6 cut(s) 238, 956, 1036, 1195, 1965, 2175
TaiI ACGT 1 cut(s) 1167
TaqII GACCGA 2 cut(s) 1343, 1587
TatI WGTACW 3 cut(s) 1428, 2510, 2662
TauI GCSGC 1 cut(s) 679
TseI GCWGC 6 cut(s) 959, 2043, 2085, 2253, 2295, 2463
TspGWI ACGGA 2 cut(s) 2246, 2636
Vha464I CTTAAG 6 cut(s) 1640, 1781, 1817, 2027, 2237, 2447
VpaK11BI GGWCC 1 cut(s) 1452
VspI ATTAAT 3 cut(s) 1977, 2187, 2397
XagI CCTNNNNNAGG 1 cut(s) 219
XapI RAATTY 7 cut(s) 346, 855, 966, 1610, 1800, 1836, 2516
XbaI TCTAGA 1 cut(s) 916
XceI RCATGY 3 cut(s) 190, 930, 1555
XcmI CCANNNNNNNNNTGG 1 cut(s) 995
XhoI CTCGAG 2 cut(s) 1391, 2708
XmnI GAANNNNTTC 2 cut(s) 9, 1155
XspI CTAG 8 cut(s) 291, 315, 591, 890, 917, 1794, 1830, 2301
ZrmI AGTACT 1 cut(s) 1430
Zsp2I ATGCAT 3 cut(s) 192, 201, 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.