Rmu_ssc0000472.1_g000013

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000472.1
OG0000067
Physical Location & Seq
Reverse (-)
71573 .. 75687
4115 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000472.1_g000013.1.cds

Sequence Viewer

Length: 2646 bp
atggcagttgctgctgcttctccatctggccgttgttggaactatgacgtcttcatcaattttagaggtgaagacacacgcaaaatctttgtcggacatctttacagagctctcgagcagaaagcaatccagacattcatcgattctgaagggcttagaaaaggcaaccatctctccaagctaattgacgctattcacgactcaaggctttcgattatagttttatcaaaaaattatgcatcttccacatggtgcttaatagaacttgtcgaaatcttggaatgtatggatgcacaaaagcaaatagtggtgcctgttttctacgatgtagatccatctgacgttcgtaagctgaagggaacttttgcagaagcttttgctaaacatgaagacggttctaacgctaacaaggaagaggtgcacagctggaagaccgctctaactagagctaccaatctatctggctgggattcacgagattacaaggacgacggtgagctcattgagaaaattgtaaaagatactcttcttaagttgattcaggtctcatcaagtaaaccacatggcttgattggaatggatgaccaccttactgaaatgtatacacttttaggtcttggggtaaatgatgttcgctttgttgggatatggggtatgggcggtataggaaaaacaacgattgccaaagctgtttatgatgaaatagcttgtcaatttgatcattcttgctttcttgatgatgtcaaggaaagtttcatgaagaagagtaaagcagaaattctagaagattttctatctagaatcttggaggaaaaggtgcagactacaggcattttaagtagaggttccaatatgataatggaaaggctaggtaagaaaaaagtgttaattgttcttgatgatttggatgatatagcccaaattgaaaccttactgggggagcaacattcatttgggggtgggagtagaatcattttgacaacaagaaatatacaatctctcagcagagttgaatatcgtttatataagcccaagttcttaagttatgataaagctcatgagctcttcatgaagtatgccttcagaatgaacaaaccctcaggagagtacgatcatctgtcaaggcgtgccataagatatgctcaaggtctgcctttagcacttaaagtcttgggagcttctctttataacaaaagtgtaccagcgtgggaagatacattagagaaaatagaggaaaccccgcaaagggaaattcaggatttccttagatcgaactttgatggactagaggcttcagagaagaacatatttctagatgttgcgtgcttctttataggagaggacagaacatatgcaacaaaaattctgaaaatttgtggcttctttcccaatattggattaggagttctagttgataaagctctggtaactattggagatggtgataagcttgagatgcataatttacttgtggaaatgggtcggaaaatagggagagccagtaggctgtggagtttcaaagattatcatgtgttgactgaaaataaggctacggaaacagttgaagccatagtcctagacttgtcgaactggaacagagaagagatatactggaacagtgaagcttttcttagcatgaaaaatctaagactgctcaagatatattggagtaactcctcagggtggtgtaaacaatacgtcagtggagacttaaagtttctttcacatcagctgaggcttcttgaatggcatagttgcccgctaaaatctttgccatgttcctttcagccgctgaatcttgtcgaccttagcatgccttatagtctcgttcaacaactttgggaaggaaccaagccgctggcaaaattgcaattcatcaatttaagtcactctcaatatcgagagaagacctccgacttgaccggggctccgattcttaagaagctaattcttgaaggttgtacaagtttattcgatatacacccatccattttgatacatgaaaatcttgtatttttaaatctgaaaggttgcagagaacttaagattcttcccagcagcatttatatgaaatctcttaggacccttaacctttctggctgctcaaatcttgaaaagtttccagagatttcaggaattatgatcgagctttcagagattgatttagatgagactgcaattaaagaactgccctcatcaattgaacagctttgggggcttgaagtattaagtatgagaaactgcagaaaccttgtctcccttccggacagtgtctgtaatttgacataccttcgacagctctatttggctgggtgctcaaaattttataacttgcctgacaatttgaggaattcgcaaacattggagatccttgaagtacaaggcagtggtatagaacaactctcattctttatgaaaaacaaagaaacagagagggagggagtcatcagagtctccagggagggaatgtcgtcatcagagttgagggaaaggaaagactacaacaacctcttgtcgctggtgccagagccacaaagtggagaaaagagaagaagagactatgaaggtggcagtgcaagtggaggagtgctcggcaggaggagggatgttgggggctttatgcagctgcttgagttgcctgagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

881

Amino Acids

100.07

Weight (kDa)

6.14

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1188, 2328
AatII GACGTC 1 cut(s) 51
AccB1I GGYRCC 2 cut(s) 310, 2521
AccB7I CCANNNNNTGG 1 cut(s) 2537
AccBSI CCGCTC 1 cut(s) 437
AccI GTMKAC 2 cut(s) 602, 1803
AccIII TCCGGA 1 cut(s) 2263
AciI CCGC 6 cut(s) 435, 660, 1241, 1760, 1790, 1856
AclWI GGATC 2 cut(s) 326, 2362
AcoI YGGCCR 1 cut(s) 28
AcsI RAATTY 6 cut(s) 777, 1251, 1362, 1371, 2321, 2350
AcuI CTGAAG 4 cut(s) 168, 374, 1066, 1278
AcyI GRCGYC 1 cut(s) 48
AdeI CACNNNGTG 2 cut(s) 252, 2537
AfaI GTAC 4 cut(s) 1109, 1200, 1963, 2379
AfiI CCNNNNNNNGG 6 cut(s) 936, 1245, 1246, 1394, 1683, 2537
AflII CTTAAG 4 cut(s) 530, 1039, 1937, 2042
AjnI CCWGG 1 cut(s) 2456
AloI GAACNNNNNNTCC 2 cut(s) 1389, 1421
Alw21I GWGCWC 6 cut(s) 112, 423, 501, 1065, 2318, 2592
Alw26I GTCTC 7 cut(s) 550, 1701, 1829, 2165, 2260, 2458, 2550
Alw44I GTGCAC 1 cut(s) 419
AlwI GGATC 2 cut(s) 326, 2362
AlwNI CAGNNNCTG 3 cut(s) 11, 1792, 2274
Ama87I CYCGRG 1 cut(s) 113
Aor13HI TCCGGA 1 cut(s) 2263
AoxI GGCC 1 cut(s) 28
ApaLI GTGCAC 1 cut(s) 419
ApeKI GCWGC 6 cut(s) 11, 14, 2058, 2100, 2623, 2626
ApoI RAATTY 6 cut(s) 777, 1251, 1362, 1371, 2321, 2350
ArsI GACNNNNNNTTYG 2 cut(s) 75, 107
Asp700I GAANNNNTTC 3 cut(s) 789, 1626, 2118
AspS9I GGNCC 1 cut(s) 2082
AsuC2I CCSGG 1 cut(s) 1924
AsuHPI GGTGA 3 cut(s) 80, 506, 1454
AvaI CYCGRG 1 cut(s) 113
AvaII GGWCC 1 cut(s) 2082
AxyI CCTNAGG 2 cut(s) 1099, 1678
BaeGI GKGCMC 1 cut(s) 423
BanI GGYRCC 2 cut(s) 310, 2521
BanII GRGCYC 4 cut(s) 112, 501, 1065, 1930
BauI CACGAG 1 cut(s) 474
BbsI GAAGAC 5 cut(s) 43, 78, 396, 437, 1913
Bbv12I GWGCWC 6 cut(s) 112, 423, 501, 1065, 2318, 2592
BbvCI CCTCAGC 1 cut(s) 1733
BbvI GCAGC 4 cut(s) 2070, 2087, 2613, 2635
BccI CCATC 6 cut(s) 31, 177, 343, 1274, 1433, 1993
BceAI ACGGC 1 cut(s) 15
BciT130I CCWGG 1 cut(s) 2458
BclI TGATCA 1 cut(s) 718
BcnI CCSGG 1 cut(s) 1924
BcoDI GTCTC 7 cut(s) 550, 1701, 1829, 2165, 2260, 2458, 2550
BfaI CTAG 8 cut(s) 444, 782, 798, 869, 1286, 1313, 1409, 1577
BfmI CTRYAG 2 cut(s) 825, 2242
BfrI CTTAAG 4 cut(s) 530, 1039, 1937, 2042
BisI GCNGC 8 cut(s) 12, 15, 1790, 1856, 2059, 2101, 2624, 2627
BlsI GCNGC 8 cut(s) 13, 16, 1791, 1857, 2060, 2102, 2625, 2628
Bme1390I CCNGG 2 cut(s) 1924, 2458
Bme18I GGWCC 1 cut(s) 2082
BmeT110I CYCGRG 1 cut(s) 113
BmgT120I GGNCC 1 cut(s) 2082
BmiI GGNNCC 6 cut(s) 312, 847, 1849, 1929, 2084, 2523
BmrFI CCNGG 2 cut(s) 1924, 2458
BmrI ACTGGG 1 cut(s) 944
BmsI GCATC 3 cut(s) 248, 280, 1446
BmuI ACTGGG 1 cut(s) 944
BpiI GAAGAC 5 cut(s) 43, 78, 396, 437, 1913
BplI GAGNNNNNCTC 4 cut(s) 1895, 1927, 2574, 2606
BpmI CTGGAG 1 cut(s) 2440
Bpu10I CCTNAGC 2 cut(s) 1733, 1808
BpuEI CTTGAG 4 cut(s) 187, 1128, 1472, 1640
BpuMI CCSGG 1 cut(s) 1924
Bsa29I ATCGAT 1 cut(s) 141
BsaBI GATNNNNATC 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 48
BsaI GGTCTC 1 cut(s) 550
BsaJI CCNNGG 2 cut(s) 1923, 2457
BsaWI WCCGGW 1 cut(s) 2263
Bsc4I CCNNNNNNNGG 6 cut(s) 936, 1245, 1246, 1394, 1683, 2537
Bse1I ACTGG 4 cut(s) 939, 1500, 1595, 1616
Bse21I CCTNAGG 2 cut(s) 1099, 1678
Bse8I GATNNNNATC 1 cut(s) 330
BseAI TCCGGA 1 cut(s) 2263
BseBI CCWGG 1 cut(s) 2458
BseCI ATCGAT 1 cut(s) 141
BseDI CCNNGG 2 cut(s) 1923, 2457
BseGI GGATG 5 cut(s) 295, 586, 913, 1985, 2611
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 6 cut(s) 936, 1245, 1246, 1394, 1683, 2537
BseMII CTCAG 5 cut(s) 1015, 1113, 1692, 1724, 2631
BseNI ACTGG 4 cut(s) 939, 1500, 1595, 1616
BseRI GAGGAG 3 cut(s) 1666, 2598, 2614
BseSI GKGCMC 1 cut(s) 423
BseXI GCAGC 4 cut(s) 2070, 2087, 2613, 2635
BseYI CCCAGC 3 cut(s) 465, 2054, 2309
BsgI GTGCAG 1 cut(s) 839
BshFI GGCC 1 cut(s) 30
BshNI GGYRCC 2 cut(s) 310, 2521
BshVI ATCGAT 1 cut(s) 141
BsiHKAI GWGCWC 6 cut(s) 112, 423, 501, 1065, 2318, 2592
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 2 cut(s) 1923, 2264
BslI CCNNNNNNNGG 6 cut(s) 936, 1245, 1246, 1394, 1683, 2537
BsmAI GTCTC 7 cut(s) 550, 1701, 1829, 2165, 2260, 2458, 2550
BsnI GGCC 1 cut(s) 30
Bso31I GGTCTC 1 cut(s) 550
BsoBI CYCGRG 1 cut(s) 113
Bsp1286I GDGCHC 7 cut(s) 112, 423, 501, 1065, 1930, 2318, 2592
Bsp13I TCCGGA 1 cut(s) 2263
Bsp1407I TGTACA 1 cut(s) 1961
Bsp143I GATC 6 cut(s) 331, 718, 1111, 1268, 2142, 2367
BspACI CCGC 6 cut(s) 435, 660, 1241, 1760, 1790, 1856
BspANI GGCC 1 cut(s) 30
BspCNI CTCAG 5 cut(s) 1014, 1112, 1691, 1725, 2632
BspDI ATCGAT 1 cut(s) 141
BspEI TCCGGA 1 cut(s) 2263
BspHI TCATGA 3 cut(s) 756, 1057, 1068
BspLI GGNNCC 6 cut(s) 312, 847, 1849, 1929, 2084, 2523
BspMAI CTGCAG 1 cut(s) 2246
BspPI GGATC 2 cut(s) 326, 2362
BspQI GCTCTTC 1 cut(s) 1070
BspT107I GGYRCC 2 cut(s) 310, 2521
BspTI CTTAAG 4 cut(s) 530, 1039, 1937, 2042
BspTNI GGTCTC 1 cut(s) 550
BsrBI CCGCTC 1 cut(s) 437
BsrGI TGTACA 1 cut(s) 1961
BsrI ACTGG 4 cut(s) 939, 1500, 1595, 1616
BssECI CCNNGG 2 cut(s) 1923, 2457
BssMI GATC 6 cut(s) 331, 718, 1111, 1268, 2142, 2367
BssNAI GTATAC 1 cut(s) 603
BssNI GRCGYC 1 cut(s) 48
BssSI CACGAG 1 cut(s) 474
Bst1107I GTATAC 1 cut(s) 603
Bst2BI CACGAG 1 cut(s) 474
Bst2UI CCWGG 1 cut(s) 2458
Bst4CI ACNGT 5 cut(s) 395, 494, 1561, 1619, 2270
Bst6I CTCTTC 6 cut(s) 408, 531, 758, 1070, 1596, 2548
BstACI GRCGYC 1 cut(s) 48
BstAFI CTTAAG 4 cut(s) 530, 1039, 1937, 2042
BstAPI GCANNNNNTGC 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 1961
BstC8I GCNNGC 5 cut(s) 1128, 1324, 1760, 1814, 1860
BstF5I GGATG 5 cut(s) 295, 586, 913, 1985, 2611
BstKTI GATC 6 cut(s) 334, 721, 1114, 1271, 2145, 2370
BstMAI GTCTC 7 cut(s) 550, 1701, 1829, 2165, 2260, 2458, 2550
BstMBI GATC 6 cut(s) 331, 718, 1111, 1268, 2142, 2367
BstMWI GCNNNNNNNGC 4 cut(s) 11, 1456, 2215, 2635
BstNI CCWGG 1 cut(s) 2458
BstNSI RCATGY 1 cut(s) 1816
BstSCI CCNGG 2 cut(s) 1922, 2456
BstSFI CTRYAG 2 cut(s) 825, 2242
BstSLI GKGCMC 1 cut(s) 423
BstV1I GCAGC 4 cut(s) 2070, 2087, 2613, 2635
BstV2I GAAGAC 5 cut(s) 43, 78, 396, 437, 1913
BstX2I RGATCY 2 cut(s) 331, 2367
BstXI CCANNNNNNTGG 1 cut(s) 1858
BstYI RGATCY 2 cut(s) 331, 2367
BstZ17I GTATAC 1 cut(s) 603
Bsu15I ATCGAT 1 cut(s) 141
Bsu36I CCTNAGG 2 cut(s) 1099, 1678
BsuRI GGCC 1 cut(s) 30
BsuTUI ATCGAT 1 cut(s) 141
BtsCI GGATG 5 cut(s) 295, 586, 913, 1985, 2611
BtsI GCAGTG 2 cut(s) 2392, 2578
BtsIMutI CAGTG 5 cut(s) 1624, 1708, 2275, 2392, 2578
Cac8I GCNNGC 5 cut(s) 1128, 1324, 1760, 1814, 1860
CaiI CAGNNNCTG 3 cut(s) 11, 1792, 2274
CciI TCATGA 3 cut(s) 756, 1057, 1068
Cfr13I GGNCC 1 cut(s) 2082
ClaI ATCGAT 1 cut(s) 141
CseI GACGC 1 cut(s) 197
Csp6I GTAC 4 cut(s) 1108, 1199, 1962, 2378
CspCI CAANNNNNGTGG 2 cut(s) 549, 584
CviQI GTAC 4 cut(s) 1108, 1199, 1962, 2378
DpnI GATC 6 cut(s) 333, 720, 1113, 1270, 2144, 2369
DpnII GATC 6 cut(s) 331, 718, 1111, 1268, 2142, 2367
DraI TTTAAA 1 cut(s) 2019
DraIII CACNNNGTG 2 cut(s) 252, 2537
EaeI YGGCCR 1 cut(s) 28
Eam1104I CTCTTC 6 cut(s) 408, 531, 758, 1070, 1596, 2548
EarI CTCTTC 6 cut(s) 408, 531, 758, 1070, 1596, 2548
Ecl136II GAGCTC 3 cut(s) 110, 499, 1063
Eco24I GRGCYC 4 cut(s) 112, 501, 1065, 1930
Eco31I GGTCTC 1 cut(s) 550
Eco47I GGWCC 1 cut(s) 2082
Eco53kI GAGCTC 3 cut(s) 110, 499, 1063
Eco57I CTGAAG 4 cut(s) 168, 374, 1066, 1278
Eco81I CCTNAGG 2 cut(s) 1099, 1678
Eco88I CYCGRG 1 cut(s) 113
EcoICRI GAGCTC 3 cut(s) 110, 499, 1063
EcoO109I RGGNCCY 1 cut(s) 2082
EcoRI GAATTC 1 cut(s) 2350
EcoRII CCWGG 1 cut(s) 2456
EcoT22I ATGCAT 2 cut(s) 241, 1461
EcoT38I GRGCYC 4 cut(s) 112, 501, 1065, 1930
FalI AAGNNNNNCTT 6 cut(s) 510, 542, 1064, 1096, 1614, 1646
FauI CCCGC 2 cut(s) 1248, 1767
FauNDI CATATG 1 cut(s) 1351
FbaI TGATCA 1 cut(s) 718
FblI GTMKAC 2 cut(s) 602, 1803
Fnu4HI GCNGC 8 cut(s) 12, 15, 1790, 1856, 2059, 2101, 2624, 2627
FokI GGATG 5 cut(s) 302, 593, 920, 1972, 2618
FriOI GRGCYC 4 cut(s) 112, 501, 1065, 1930
Fsp4HI GCNGC 8 cut(s) 12, 15, 1790, 1856, 2059, 2101, 2624, 2627
FspBI CTAG 8 cut(s) 444, 782, 798, 869, 1286, 1313, 1409, 1577
GluI GCNGC 8 cut(s) 12, 15, 1790, 1856, 2059, 2101, 2624, 2627
GsaI CCCAGC 3 cut(s) 469, 2058, 2313
GsuI CTGGAG 1 cut(s) 2440
HaeIII GGCC 1 cut(s) 30
HapII CCGG 2 cut(s) 1923, 2264
HgaI GACGC 1 cut(s) 197
Hin1I GRCGYC 1 cut(s) 48
HincII GTYRAC 2 cut(s) 1536, 1804
HindII GTYRAC 2 cut(s) 1536, 1804
HindIII AAGCTT 3 cut(s) 372, 1448, 1623
HpaII CCGG 2 cut(s) 1923, 2264
HphI GGTGA 3 cut(s) 80, 506, 1454
Hpy166II GTNNAC 7 cut(s) 421, 557, 603, 1199, 1536, 1691, 1804
Hpy8I GTNNAC 7 cut(s) 421, 557, 603, 1199, 1536, 1691, 1804
Hpy99I CGWCG 1 cut(s) 494
HpyAV CCTTC 8 cut(s) 143, 349, 1090, 1838, 1949, 2270, 2300, 2558
HpyCH4III ACNGT 5 cut(s) 395, 494, 1561, 1619, 2270
HpyCH4IV ACGT 3 cut(s) 48, 342, 1698
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 1456, 2215, 2635
HpySE526I ACGT 3 cut(s) 48, 342, 1698
Hsp92I GRCGYC 1 cut(s) 48
Kpn2I TCCGGA 1 cut(s) 2263
Ksp22I TGATCA 1 cut(s) 718
Kzo9I GATC 6 cut(s) 331, 718, 1111, 1268, 2142, 2367
LguI GCTCTTC 1 cut(s) 1070
LmnI GCTCC 3 cut(s) 940, 1175, 1933
Lsp1109I GCAGC 4 cut(s) 2070, 2087, 2613, 2635
LweI GCATC 3 cut(s) 248, 280, 1446
MaeI CTAG 8 cut(s) 444, 782, 798, 869, 1286, 1313, 1409, 1577
MaeII ACGT 3 cut(s) 48, 342, 1698
MaeIII GTNAC 3 cut(s) 1426, 1670, 1886
MalI GATC 6 cut(s) 333, 720, 1113, 1270, 2144, 2369
MbiI CCGCTC 1 cut(s) 437
MboI GATC 6 cut(s) 331, 718, 1111, 1268, 2142, 2367
MfeI CAATTG 1 cut(s) 2199
MflI RGATCY 2 cut(s) 331, 2367
MhlI GDGCHC 7 cut(s) 112, 423, 501, 1065, 1930, 2318, 2592
MlyI GAGTC 3 cut(s) 194, 2451, 2460
MmeI TCCRAC 4 cut(s) 17, 73, 1463, 1938
Mph1103I ATGCAT 2 cut(s) 241, 1461
MroI TCCGGA 1 cut(s) 2263
MroXI GAANNNNTTC 3 cut(s) 789, 1626, 2118
MslI CAYNNNNRTG 1 cut(s) 2066
MspA1I CMGCKG 5 cut(s) 426, 1732, 1792, 1858, 2626
MspCI CTTAAG 4 cut(s) 530, 1039, 1937, 2042
MspI CCGG 2 cut(s) 1923, 2264
MspR9I CCNGG 2 cut(s) 1924, 2458
MunI CAATTG 1 cut(s) 2199
MvaI CCWGG 1 cut(s) 2458
MwoI GCNNNNNNNGC 4 cut(s) 11, 1456, 2215, 2635
NciI CCSGG 1 cut(s) 1924
NdeI CATATG 1 cut(s) 1351
NdeII GATC 6 cut(s) 331, 718, 1111, 1268, 2142, 2367
NlaIV GGNNCC 6 cut(s) 312, 847, 1849, 1929, 2084, 2523
NmeAIII GCCGAG 1 cut(s) 2571
NmuCI GTSAC 1 cut(s) 1886
NsiI ATGCAT 2 cut(s) 241, 1461
NspI RCATGY 1 cut(s) 1816
PaeI GCATGC 1 cut(s) 1816
PaeR7I CTCGAG 1 cut(s) 113
PagI TCATGA 3 cut(s) 756, 1057, 1068
PciSI GCTCTTC 1 cut(s) 1070
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PdmI GAANNNNTTC 3 cut(s) 789, 1626, 2118
PfeI GAWTC 8 cut(s) 143, 470, 538, 801, 969, 1795, 1933, 2047
PflFI GACNNNGTC 1 cut(s) 2270
PflMI CCANNNNNTGG 1 cut(s) 2537
PkrI GCNGC 8 cut(s) 13, 16, 1791, 1857, 2060, 2102, 2625, 2628
PleI GAGTC 3 cut(s) 194, 2450, 2459
PpsI GAGTC 3 cut(s) 194, 2450, 2459
PpuMI RGGWCCY 1 cut(s) 2082
PsiI TTATAA 2 cut(s) 1188, 2328
Psp124BI GAGCTC 3 cut(s) 112, 501, 1065
Psp5II RGGWCCY 1 cut(s) 2082
Psp6I CCWGG 1 cut(s) 2456
PspFI CCCAGC 3 cut(s) 465, 2054, 2309
PspGI CCWGG 1 cut(s) 2456
PspN4I GGNNCC 6 cut(s) 312, 847, 1849, 1929, 2084, 2523
PspPI GGNCC 1 cut(s) 2082
PspPPI RGGWCCY 1 cut(s) 2082
PsrI GAACNNNNNNTAC 2 cut(s) 615, 647
PstI CTGCAG 1 cut(s) 2246
PstNI CAGNNNCTG 3 cut(s) 11, 1792, 2274
PsuI RGATCY 2 cut(s) 331, 2367
PsyI GACNNNGTC 1 cut(s) 2270
PvuII CAGCTG 3 cut(s) 426, 1732, 2626
RsaI GTAC 4 cut(s) 1109, 1200, 1963, 2379
RsaNI GTAC 4 cut(s) 1108, 1199, 1962, 2378
RseI CAYNNNNRTG 1 cut(s) 2066
SacI GAGCTC 3 cut(s) 112, 501, 1065
SalI GTCGAC 1 cut(s) 1802
SapI GCTCTTC 1 cut(s) 1070
SatI GCNGC 8 cut(s) 12, 15, 1790, 1856, 2059, 2101, 2624, 2627
Sau3AI GATC 6 cut(s) 331, 718, 1111, 1268, 2142, 2367
Sau96I GGNCC 1 cut(s) 2082
SchI GAGTC 3 cut(s) 194, 2451, 2460
ScrFI CCNGG 2 cut(s) 1924, 2458
SduI GDGCHC 7 cut(s) 112, 423, 501, 1065, 1930, 2318, 2592
SfaNI GCATC 3 cut(s) 248, 280, 1446
SfcI CTRYAG 2 cut(s) 825, 2242
Sfr274I CTCGAG 1 cut(s) 113
SinI GGWCC 1 cut(s) 2082
SlaI CTCGAG 1 cut(s) 113
SmiMI CAYNNNNRTG 1 cut(s) 2066
SphI GCATGC 1 cut(s) 1816
SsiI CCGC 6 cut(s) 435, 660, 1241, 1760, 1790, 1856
SspI AATATT 1 cut(s) 1393
SspMI CTAG 8 cut(s) 444, 782, 798, 869, 1286, 1313, 1409, 1577
SstI GAGCTC 3 cut(s) 112, 501, 1065
StyD4I CCNGG 2 cut(s) 1922, 2456
TaaI ACNGT 5 cut(s) 395, 494, 1561, 1619, 2270
TaiI ACGT 3 cut(s) 51, 345, 1701
TatI WGTACW 2 cut(s) 1961, 2377
TauI GCSGC 2 cut(s) 1792, 1858
TfiI GAWTC 8 cut(s) 143, 470, 538, 801, 969, 1795, 1933, 2047
TscAI CASTG 5 cut(s) 1624, 1708, 2275, 2392, 2578
TseFI GTSAC 1 cut(s) 1886
TseI GCWGC 6 cut(s) 11, 14, 2058, 2100, 2623, 2626
Tsp45I GTSAC 1 cut(s) 1886
TspGWI ACGGA 1 cut(s) 1568
TspRI CASTG 5 cut(s) 1624, 1708, 2275, 2392, 2578
Tth111I GACNNNGTC 1 cut(s) 2270
Van91I CCANNNNNTGG 1 cut(s) 2537
Vha464I CTTAAG 4 cut(s) 530, 1039, 1937, 2042
VneI GTGCAC 1 cut(s) 419
VpaK11BI GGWCC 1 cut(s) 2082
XapI RAATTY 6 cut(s) 777, 1251, 1362, 1371, 2321, 2350
XbaI TCTAGA 3 cut(s) 781, 797, 1312
XceI RCATGY 1 cut(s) 1816
XcmI CCANNNNNNNNNTGG 1 cut(s) 856
XhoI CTCGAG 1 cut(s) 113
XmiI GTMKAC 2 cut(s) 602, 1803
XmnI GAANNNNTTC 3 cut(s) 789, 1626, 2118
XspI CTAG 8 cut(s) 444, 782, 798, 869, 1286, 1313, 1409, 1577
ZraI GACGTC 1 cut(s) 49
Zsp2I ATGCAT 2 cut(s) 241, 1461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.