TMV resistance protein N-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 723 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 2776 |
| AatII | GACGTC | 1 cut(s) | 4210 |
| Acc16I | TGCGCA | 1 cut(s) | 1646 |
| Acc36I | ACCTGC | 3 cut(s) | 1303, 2641, 4278 |
| AccB1I | GGYRCC | 1 cut(s) | 2031 |
| AccI | GTMKAC | 2 cut(s) | 147, 1498 |
| AccII | CGCG | 1 cut(s) | 1158 |
| AccIII | TCCGGA | 1 cut(s) | 1576 |
| AclI | AACGTT | 2 cut(s) | 964, 2600 |
| AcoI | YGGCCR | 3 cut(s) | 2486, 4110, 4291 |
| AcsI | RAATTY | 6 cut(s) | 253, 729, 1864, 2372, 2925, 3299 |
| AcuI | CTGAAG | 4 cut(s) | 1063, 2198, 2447, 3828 |
| AcyI | GRCGYC | 1 cut(s) | 4207 |
| AdeI | CACNNNGTG | 1 cut(s) | 264 |
| AfaI | GTAC | 6 cut(s) | 924, 2382, 2512, 2832, 3115, 3140 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 187, 711, 1702, 2464, 2879 |
| AflII | CTTAAG | 3 cut(s) | 478, 1937, 2042 |
| AflIII | ACRYGT | 3 cut(s) | 1012, 2235, 3216 |
| AjiI | CACGTC | 1 cut(s) | 1810 |
| AjnI | CCWGG | 5 cut(s) | 710, 1111, 2914, 3042, 4243 |
| AleI | CACNNNNGTG | 1 cut(s) | 2388 |
| Alw21I | GWGCWC | 4 cut(s) | 1438, 2152, 3476, 3562 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2277 |
| Ama87I | CYCGRG | 2 cut(s) | 1043, 3203 |
| Aor13HI | TCCGGA | 1 cut(s) | 1576 |
| AoxI | GGCC | 6 cut(s) | 1089, 1405, 2486, 3294, 4110, 4291 |
| ApeKI | GCWGC | 4 cut(s) | 2058, 2103, 2468, 3974 |
| ApoI | RAATTY | 6 cut(s) | 253, 729, 1864, 2372, 2925, 3299 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2241, 2273 |
| AseI | ATTAAT | 1 cut(s) | 3033 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 1076, 1091, 2211 |
| AspLEI | GCGC | 5 cut(s) | 405, 649, 1647, 3551, 4044 |
| AspS9I | GGNCC | 2 cut(s) | 4, 4247 |
| AsuC2I | CCSGG | 2 cut(s) | 1404, 4378 |
| AvaI | CYCGRG | 2 cut(s) | 1043, 3203 |
| AvaII | GGWCC | 2 cut(s) | 4, 4247 |
| AxyI | CCTNAGG | 1 cut(s) | 2877 |
| BalI | TGGCCA | 1 cut(s) | 2488 |
| BamHI | GGATCC | 2 cut(s) | 1625, 1847 |
| BanI | GGYRCC | 1 cut(s) | 2031 |
| BanII | GRGCYC | 1 cut(s) | 1438 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2944, 2976 |
| BauI | CACGAG | 1 cut(s) | 1722 |
| BbsI | GAAGAC | 8 cut(s) | 58, 93, 1626, 2042, 2952, 3793, 4136, 4255 |
| Bbv12I | GWGCWC | 4 cut(s) | 1438, 2152, 3476, 3562 |
| BbvI | GCAGC | 4 cut(s) | 2070, 2090, 2455, 3961 |
| BceAI | ACGGC | 6 cut(s) | 1587, 2019, 2225, 3267, 4237, 4306 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 2236, 2270, 2552, 2586 |
| BciT130I | CCWGG | 5 cut(s) | 712, 1113, 2916, 3044, 4245 |
| BclI | TGATCA | 1 cut(s) | 2065 |
| BcnI | CCSGG | 2 cut(s) | 1404, 4378 |
| BfaI | CTAG | 8 cut(s) | 905, 1002, 1298, 1325, 1424, 2055, 3486, 4418 |
| BfrI | CTTAAG | 3 cut(s) | 478, 1937, 2042 |
| BfuAI | ACCTGC | 3 cut(s) | 1303, 2641, 4278 |
| BglII | AGATCT | 2 cut(s) | 163, 2297 |
| BisI | GCNGC | 8 cut(s) | 471, 688, 2059, 2104, 2469, 3975, 4110, 4294 |
| BlpI | GCTNAGC | 2 cut(s) | 1548, 3770 |
| BlsI | GCNGC | 8 cut(s) | 472, 689, 2060, 2105, 2470, 3976, 4111, 4295 |
| Bme1390I | CCNGG | 7 cut(s) | 712, 1113, 1404, 2916, 3044, 4245, 4378 |
| Bme18I | GGWCC | 2 cut(s) | 4, 4247 |
| BmeT110I | CYCGRG | 2 cut(s) | 1043, 3203 |
| BmgBI | CACGTC | 1 cut(s) | 1810 |
| BmgT120I | GGNCC | 2 cut(s) | 4, 4247 |
| BmiI | GGNNCC | 6 cut(s) | 6, 1575, 1627, 1849, 2033, 3429 |
| BmrFI | CCNGG | 7 cut(s) | 712, 1113, 1404, 2916, 3044, 4245, 4378 |
| BmrI | ACTGGG | 3 cut(s) | 1228, 1710, 3233 |
| BmuI | ACTGGG | 3 cut(s) | 1228, 1710, 3233 |
| BoxI | GACNNNNGTC | 4 cut(s) | 4210, 4252, 4434, 4513 |
| BpiI | GAAGAC | 8 cut(s) | 58, 93, 1626, 2042, 2952, 3793, 4136, 4255 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 1876, 1908, 3458, 3490, 3601, 3633 |
| BpmI | CTGGAG | 4 cut(s) | 1134, 1695, 4459, 4538 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1168, 3954 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1548, 3770 |
| BpuEI | CTTGAG | 3 cut(s) | 1217, 1487, 2135 |
| BpuMI | CCSGG | 2 cut(s) | 1404, 4378 |
| Bsa29I | ATCGAT | 3 cut(s) | 1878, 4423, 4502 |
| BsaAI | YACGTR | 2 cut(s) | 890, 2537 |
| BsaBI | GATNNNNATC | 4 cut(s) | 612, 861, 1874, 2544 |
| BsaHI | GRCGYC | 1 cut(s) | 4207 |
| BsaI | GGTCTC | 3 cut(s) | 445, 4218, 4350 |
| BsaJI | CCNNGG | 5 cut(s) | 711, 1111, 2195, 3042, 4376 |
| BsaWI | WCCGGW | 5 cut(s) | 1576, 4356, 4399, 4464, 4543 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 187, 711, 1702, 2464, 2879 |
| Bse118I | RCCGGY | 1 cut(s) | 1855 |
| Bse21I | CCTNAGG | 1 cut(s) | 2877 |
| Bse3DI | GCAATG | 6 cut(s) | 204, 609, 705, 3054, 3130, 3347 |
| Bse8I | GATNNNNATC | 4 cut(s) | 612, 861, 1874, 2544 |
| BseAI | TCCGGA | 1 cut(s) | 1576 |
| BseBI | CCWGG | 5 cut(s) | 712, 1113, 2916, 3044, 4245 |
| BseCI | ATCGAT | 3 cut(s) | 1878, 4423, 4502 |
| BseDI | CCNNGG | 5 cut(s) | 711, 1111, 2195, 3042, 4376 |
| BseJI | GATNNNNATC | 4 cut(s) | 612, 861, 1874, 2544 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 187, 711, 1702, 2464, 2879 |
| BseMI | GCAATG | 6 cut(s) | 204, 609, 705, 3054, 3130, 3347 |
| BseRI | GAGGAG | 4 cut(s) | 314, 2865, 3472, 4343 |
| BseX3I | CGGCCG | 2 cut(s) | 4110, 4291 |
| BseXI | GCAGC | 4 cut(s) | 2070, 2090, 2455, 3961 |
| BseYI | CCCAGC | 1 cut(s) | 486 |
| BsgI | GTGCAG | 2 cut(s) | 302, 461 |
| Bsh1236I | CGCG | 1 cut(s) | 1158 |
| Bsh1285I | CGRYCG | 6 cut(s) | 181, 2797, 3187, 4113, 4227, 4294 |
| BshFI | GGCC | 6 cut(s) | 1091, 1407, 2488, 3296, 4112, 4293 |
| BshNI | GGYRCC | 1 cut(s) | 2031 |
| BshVI | ATCGAT | 3 cut(s) | 1878, 4423, 4502 |
| BsiEI | CGRYCG | 6 cut(s) | 181, 2797, 3187, 4113, 4227, 4294 |
| BsiHKAI | GWGCWC | 4 cut(s) | 1438, 2152, 3476, 3562 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1043, 3203 |
| BsiSI | CCGG | 9 cut(s) | 650, 1404, 1577, 1856, 4357, 4378, 4400, 4465, 4544 |
| BslFI | GGGAC | 4 cut(s) | 642, 1386, 1602, 1718 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 187, 711, 1702, 2464, 2879 |
| BsmBI | CGTCTC | 1 cut(s) | 4260 |
| BsmFI | GGGAC | 4 cut(s) | 642, 1386, 1602, 1718 |
| BsnI | GGCC | 6 cut(s) | 1091, 1407, 2488, 3296, 4112, 4293 |
| Bso31I | GGTCTC | 3 cut(s) | 445, 4218, 4350 |
| BsoBI | CYCGRG | 2 cut(s) | 1043, 3203 |
| Bsp1286I | GDGCHC | 4 cut(s) | 1438, 2152, 3476, 3562 |
| Bsp13I | TCCGGA | 1 cut(s) | 1576 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1548, 3770 |
| Bsp19I | CCATGG | 1 cut(s) | 2195 |
| BspANI | GGCC | 6 cut(s) | 1091, 1407, 2488, 3296, 4112, 4293 |
| BspDI | ATCGAT | 3 cut(s) | 1878, 4423, 4502 |
| BspEI | TCCGGA | 1 cut(s) | 1576 |
| BspFNI | CGCG | 1 cut(s) | 1158 |
| BspHI | TCATGA | 1 cut(s) | 2640 |
| BspLI | GGNNCC | 6 cut(s) | 6, 1575, 1627, 1849, 2033, 3429 |
| BspMI | ACCTGC | 3 cut(s) | 1303, 2641, 4278 |
| BspT107I | GGYRCC | 1 cut(s) | 2031 |
| BspTI | CTTAAG | 3 cut(s) | 478, 1937, 2042 |
| BspTNI | GGTCTC | 3 cut(s) | 445, 4218, 4350 |
| BsrDI | GCAATG | 6 cut(s) | 204, 609, 705, 3054, 3130, 3347 |
| BsrFI | RCCGGY | 1 cut(s) | 1855 |
| BssAI | RCCGGY | 1 cut(s) | 1855 |
| BssECI | CCNNGG | 5 cut(s) | 711, 1111, 2195, 3042, 4376 |
| BssNI | GRCGYC | 1 cut(s) | 4207 |
| BssSI | CACGAG | 1 cut(s) | 1722 |
| BssT1I | CCWWGG | 1 cut(s) | 2195 |
| Bst2BI | CACGAG | 1 cut(s) | 1722 |
| Bst2UI | CCWGG | 5 cut(s) | 712, 1113, 2916, 3044, 4245 |
| Bst6I | CTCTTC | 3 cut(s) | 22, 1082, 3866 |
| BstACI | GRCGYC | 1 cut(s) | 4207 |
| BstAFI | CTTAAG | 3 cut(s) | 478, 1937, 2042 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1151 |
| BstBAI | YACGTR | 2 cut(s) | 890, 2537 |
| BstC8I | GCNNGC | 6 cut(s) | 1156, 1257, 1857, 2101, 2245, 4304 |
| BstDSI | CCRYGG | 1 cut(s) | 2195 |
| BstFNI | CGCG | 1 cut(s) | 1158 |
| BstHHI | GCGC | 5 cut(s) | 405, 649, 1647, 3551, 4044 |
| BstMCI | CGRYCG | 6 cut(s) | 181, 2797, 3187, 4113, 4227, 4294 |
| BstNI | CCWGG | 5 cut(s) | 712, 1113, 2916, 3044, 4245 |
| BstNSI | RCATGY | 5 cut(s) | 1016, 1104, 1338, 2239, 3220 |
| BstPAI | GACNNNNGTC | 4 cut(s) | 4210, 4252, 4434, 4513 |
| BstSCI | CCNGG | 7 cut(s) | 710, 1111, 1402, 2914, 3042, 4243, 4376 |
| BstSNI | TACGTA | 1 cut(s) | 890 |
| BstUI | CGCG | 1 cut(s) | 1158 |
| BstV1I | GCAGC | 4 cut(s) | 2070, 2090, 2455, 3961 |
| BstV2I | GAAGAC | 8 cut(s) | 58, 93, 1626, 2042, 2952, 3793, 4136, 4255 |
| BstX2I | RGATCY | 8 cut(s) | 163, 190, 343, 570, 1625, 1847, 1889, 2297 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1955, 2988 |
| BstYI | RGATCY | 8 cut(s) | 163, 190, 343, 570, 1625, 1847, 1889, 2297 |
| BstZI | CGGCCG | 2 cut(s) | 4110, 4291 |
| Bsu15I | ATCGAT | 3 cut(s) | 1878, 4423, 4502 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2877 |
| BsuRI | GGCC | 6 cut(s) | 1091, 1407, 2488, 3296, 4112, 4293 |
| BsuTUI | ATCGAT | 3 cut(s) | 1878, 4423, 4502 |
| BtgI | CCRYGG | 1 cut(s) | 2195 |
| BtrI | CACGTC | 1 cut(s) | 1810 |
| BtsIMutI | CAGTG | 5 cut(s) | 1216, 2150, 2954, 3492, 3978 |
| BveI | ACCTGC | 3 cut(s) | 1303, 2641, 4278 |
| Cac8I | GCNNGC | 6 cut(s) | 1156, 1257, 1857, 2101, 2245, 4304 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2277 |
| CciI | TCATGA | 1 cut(s) | 2640 |
| CfoI | GCGC | 5 cut(s) | 405, 649, 1647, 3551, 4044 |
| Cfr10I | RCCGGY | 1 cut(s) | 1855 |
| Cfr13I | GGNCC | 2 cut(s) | 4, 4247 |
| ClaI | ATCGAT | 3 cut(s) | 1878, 4423, 4502 |
| CseI | GACGC | 3 cut(s) | 608, 1599, 3640 |
| Csp6I | GTAC | 6 cut(s) | 923, 2381, 2511, 2831, 3114, 3139 |
| CviQI | GTAC | 6 cut(s) | 923, 2381, 2511, 2831, 3114, 3139 |
| DraI | TTTAAA | 4 cut(s) | 1345, 2169, 3544, 3688 |
| DraIII | CACNNNGTG | 1 cut(s) | 264 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2776 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2776 |
| EaeI | YGGCCR | 3 cut(s) | 2486, 4110, 4291 |
| EagI | CGGCCG | 2 cut(s) | 4110, 4291 |
| Eam1104I | CTCTTC | 3 cut(s) | 22, 1082, 3866 |
| EarI | CTCTTC | 3 cut(s) | 22, 1082, 3866 |
| Ecl136II | GAGCTC | 1 cut(s) | 1436 |
| EclXI | CGGCCG | 2 cut(s) | 4110, 4291 |
| Eco105I | TACGTA | 1 cut(s) | 890 |
| Eco130I | CCWWGG | 1 cut(s) | 2195 |
| Eco147I | AGGCCT | 1 cut(s) | 1091 |
| Eco24I | GRGCYC | 1 cut(s) | 1438 |
| Eco31I | GGTCTC | 3 cut(s) | 445, 4218, 4350 |
| Eco32I | GATATC | 1 cut(s) | 1754 |
| Eco47I | GGWCC | 2 cut(s) | 4, 4247 |
| Eco52I | CGGCCG | 2 cut(s) | 4110, 4291 |
| Eco53kI | GAGCTC | 1 cut(s) | 1436 |
| Eco57I | CTGAAG | 4 cut(s) | 1063, 2198, 2447, 3828 |
| Eco81I | CCTNAGG | 1 cut(s) | 2877 |
| Eco88I | CYCGRG | 2 cut(s) | 1043, 3203 |
| EcoICRI | GAGCTC | 1 cut(s) | 1436 |
| EcoRI | GAATTC | 1 cut(s) | 253 |
| EcoRII | CCWGG | 5 cut(s) | 710, 1111, 2914, 3042, 4243 |
| EcoRV | GATATC | 1 cut(s) | 1754 |
| EcoT14I | CCWWGG | 1 cut(s) | 2195 |
| EcoT22I | ATGCAT | 1 cut(s) | 3374 |
| EcoT38I | GRGCYC | 1 cut(s) | 1438 |
| ErhI | CCWWGG | 1 cut(s) | 2195 |
| Esp3I | CGTCTC | 1 cut(s) | 4260 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 150, 182 |
| FaqI | GGGAC | 4 cut(s) | 642, 1386, 1602, 1718 |
| FauI | CCCGC | 3 cut(s) | 463, 1579, 4090 |
| FbaI | TGATCA | 1 cut(s) | 2065 |
| FblI | GTMKAC | 2 cut(s) | 147, 1498 |
| Fnu4HI | GCNGC | 8 cut(s) | 471, 688, 2059, 2104, 2469, 3975, 4110, 4294 |
| FriOI | GRGCYC | 1 cut(s) | 1438 |
| Fsp4HI | GCNGC | 8 cut(s) | 471, 688, 2059, 2104, 2469, 3975, 4110, 4294 |
| FspAI | RTGCGCAY | 1 cut(s) | 1646 |
| FspBI | CTAG | 8 cut(s) | 905, 1002, 1298, 1325, 1424, 2055, 3486, 4418 |
| FspI | TGCGCA | 1 cut(s) | 1646 |
| GlaI | GCGC | 5 cut(s) | 404, 648, 1646, 3550, 4043 |
| GluI | GCNGC | 8 cut(s) | 471, 688, 2059, 2104, 2469, 3975, 4110, 4294 |
| GsaI | CCCAGC | 1 cut(s) | 490 |
| GsuI | CTGGAG | 4 cut(s) | 1134, 1695, 4459, 4538 |
| HaeIII | GGCC | 6 cut(s) | 1091, 1407, 2488, 3296, 4112, 4293 |
| HapII | CCGG | 9 cut(s) | 650, 1404, 1577, 1856, 4357, 4378, 4400, 4465, 4544 |
| HgaI | GACGC | 3 cut(s) | 608, 1599, 3640 |
| HhaI | GCGC | 5 cut(s) | 405, 649, 1647, 3551, 4044 |
| Hin1I | GRCGYC | 1 cut(s) | 4207 |
| Hin6I | GCGC | 5 cut(s) | 403, 647, 1645, 3549, 4042 |
| HinP1I | GCGC | 5 cut(s) | 403, 647, 1645, 3549, 4042 |
| HincII | GTYRAC | 2 cut(s) | 847, 4405 |
| HindII | GTYRAC | 2 cut(s) | 847, 4405 |
| HindIII | AAGCTT | 5 cut(s) | 218, 384, 1934, 2159, 2666 |
| HpaII | CCGG | 9 cut(s) | 650, 1404, 1577, 1856, 4357, 4378, 4400, 4465, 4544 |
| Hpy99I | CGWCG | 3 cut(s) | 3719, 4212, 4281 |
| HpyCH4IV | ACGT | 9 cut(s) | 889, 964, 1272, 1809, 2333, 2422, 2536, 2600, 4207 |
| HpySE526I | ACGT | 9 cut(s) | 889, 964, 1272, 1809, 2333, 2422, 2536, 2600, 4207 |
| Hsp92I | GRCGYC | 1 cut(s) | 4207 |
| HspAI | GCGC | 5 cut(s) | 403, 647, 1645, 3549, 4042 |
| Kpn2I | TCCGGA | 1 cut(s) | 1576 |
| KroI | GCCGGC | 1 cut(s) | 1855 |
| KroNI | GCCGGC | 1 cut(s) | 1857 |
| Ksp22I | TGATCA | 1 cut(s) | 2065 |
| LmnI | GCTCC | 4 cut(s) | 1579, 2147, 2476, 3459 |
| Lsp1109I | GCAGC | 4 cut(s) | 2070, 2090, 2455, 3961 |
| MaeI | CTAG | 8 cut(s) | 905, 1002, 1298, 1325, 1424, 2055, 3486, 4418 |
| MaeII | ACGT | 9 cut(s) | 889, 964, 1272, 1809, 2333, 2422, 2536, 2600, 4207 |
| MfeI | CAATTG | 5 cut(s) | 1384, 2069, 2202, 2496, 3311 |
| MflI | RGATCY | 8 cut(s) | 163, 190, 343, 570, 1625, 1847, 1889, 2297 |
| MhlI | GDGCHC | 4 cut(s) | 1438, 2152, 3476, 3562 |
| MlsI | TGGCCA | 1 cut(s) | 2488 |
| MluNI | TGGCCA | 1 cut(s) | 2488 |
| MlyI | GAGTC | 6 cut(s) | 1915, 2056, 2307, 3215, 3332, 3337 |
| MmeI | TCCRAC | 5 cut(s) | 649, 858, 1800, 2349, 2532 |
| Mox20I | TGGCCA | 1 cut(s) | 2488 |
| Mph1103I | ATGCAT | 1 cut(s) | 3374 |
| MroI | TCCGGA | 1 cut(s) | 1576 |
| MroNI | GCCGGC | 1 cut(s) | 1855 |
| MroXI | GAANNNNTTC | 3 cut(s) | 1076, 1091, 2211 |
| MscI | TGGCCA | 1 cut(s) | 2488 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1953, 2388 |
| Msp20I | TGGCCA | 1 cut(s) | 2488 |
| MspA1I | CMGCKG | 4 cut(s) | 473, 2103, 2827, 4296 |
| MspCI | CTTAAG | 3 cut(s) | 478, 1937, 2042 |
| MspI | CCGG | 9 cut(s) | 650, 1404, 1577, 1856, 4357, 4378, 4400, 4465, 4544 |
| MspR9I | CCNGG | 7 cut(s) | 712, 1113, 1404, 2916, 3044, 4245, 4378 |
| MunI | CAATTG | 5 cut(s) | 1384, 2069, 2202, 2496, 3311 |
| MvaI | CCWGG | 5 cut(s) | 712, 1113, 2916, 3044, 4245 |
| MvnI | CGCG | 1 cut(s) | 1158 |
| NaeI | GCCGGC | 1 cut(s) | 1857 |
| NciI | CCSGG | 2 cut(s) | 1404, 4378 |
| NcoI | CCATGG | 1 cut(s) | 2195 |
| NgoMIV | GCCGGC | 1 cut(s) | 1855 |
| NlaIV | GGNNCC | 6 cut(s) | 6, 1575, 1627, 1849, 2033, 3429 |
| NmeAIII | GCCGAG | 2 cut(s) | 3455, 4138 |
| NmuCI | GTSAC | 2 cut(s) | 1388, 3995 |
| NsbI | TGCGCA | 1 cut(s) | 1646 |
| NsiI | ATGCAT | 1 cut(s) | 3374 |
| NspI | RCATGY | 5 cut(s) | 1016, 1104, 1338, 2239, 3220 |
| OliI | CACNNNNGTG | 1 cut(s) | 2388 |
| PaeR7I | CTCGAG | 1 cut(s) | 3203 |
| PagI | TCATGA | 1 cut(s) | 2640 |
| PceI | AGGCCT | 1 cut(s) | 1091 |
| PciI | ACATGT | 3 cut(s) | 1012, 2235, 3216 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 924 |
| PdiI | GCCGGC | 1 cut(s) | 1857 |
| PdmI | GAANNNNTTC | 3 cut(s) | 1076, 1091, 2211 |
| PfoI | TCCNGGA | 1 cut(s) | 4243 |
| PkrI | GCNGC | 8 cut(s) | 472, 689, 2060, 2105, 2470, 3976, 4111, 4295 |
| Ple19I | CGATCG | 1 cut(s) | 4227 |
| PleI | GAGTC | 6 cut(s) | 1915, 2055, 2307, 3214, 3331, 3336 |
| PpsI | GAGTC | 6 cut(s) | 1915, 2055, 2307, 3214, 3331, 3336 |
| Ppu21I | YACGTR | 2 cut(s) | 890, 2537 |
| PscI | ACATGT | 3 cut(s) | 1012, 2235, 3216 |
| PshAI | GACNNNNGTC | 4 cut(s) | 4210, 4252, 4434, 4513 |
| PshBI | ATTAAT | 1 cut(s) | 3033 |
| PsiI | TTATAA | 1 cut(s) | 723 |
| Psp124BI | GAGCTC | 1 cut(s) | 1438 |
| Psp1406I | AACGTT | 2 cut(s) | 964, 2600 |
| Psp6I | CCWGG | 5 cut(s) | 710, 1111, 2914, 3042, 4243 |
| PspFI | CCCAGC | 1 cut(s) | 486 |
| PspGI | CCWGG | 5 cut(s) | 710, 1111, 2914, 3042, 4243 |
| PspN4I | GGNNCC | 6 cut(s) | 6, 1575, 1627, 1849, 2033, 3429 |
| PspPI | GGNCC | 2 cut(s) | 4, 4247 |
| PspXI | VCTCGAGB | 1 cut(s) | 3203 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1359, 1391 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2277 |
| PsuI | RGATCY | 8 cut(s) | 163, 190, 343, 570, 1625, 1847, 1889, 2297 |
| PvuI | CGATCG | 1 cut(s) | 4227 |
| PvuII | CAGCTG | 2 cut(s) | 2103, 2827 |
| RsaI | GTAC | 6 cut(s) | 924, 2382, 2512, 2832, 3115, 3140 |
| RsaNI | GTAC | 6 cut(s) | 923, 2381, 2511, 2831, 3114, 3139 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1953, 2388 |
| SacI | GAGCTC | 1 cut(s) | 1438 |
| SatI | GCNGC | 8 cut(s) | 471, 688, 2059, 2104, 2469, 3975, 4110, 4294 |
| Sau96I | GGNCC | 2 cut(s) | 4, 4247 |
| SchI | GAGTC | 6 cut(s) | 1915, 2056, 2307, 3215, 3332, 3337 |
| ScrFI | CCNGG | 7 cut(s) | 712, 1113, 1404, 2916, 3044, 4245, 4378 |
| SduI | GDGCHC | 4 cut(s) | 1438, 2152, 3476, 3562 |
| Sfr274I | CTCGAG | 1 cut(s) | 3203 |
| SinI | GGWCC | 2 cut(s) | 4, 4247 |
| SlaI | CTCGAG | 1 cut(s) | 3203 |
| SmiI | ATTTAAAT | 1 cut(s) | 2169 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1953, 2388 |
| SmlI | CTYRAG | 7 cut(s) | 478, 1232, 1466, 1937, 2042, 2114, 3203 |
| SmoI | CTYRAG | 7 cut(s) | 478, 1232, 1466, 1937, 2042, 2114, 3203 |
| SnaBI | TACGTA | 1 cut(s) | 890 |
| SseBI | AGGCCT | 1 cut(s) | 1091 |
| SspMI | CTAG | 8 cut(s) | 905, 1002, 1298, 1325, 1424, 2055, 3486, 4418 |
| SstI | GAGCTC | 1 cut(s) | 1438 |
| StuI | AGGCCT | 1 cut(s) | 1091 |
| StyD4I | CCNGG | 7 cut(s) | 710, 1111, 1402, 2914, 3042, 4243, 4376 |
| StyI | CCWWGG | 1 cut(s) | 2195 |
| SwaI | ATTTAAAT | 1 cut(s) | 2169 |
| TaiI | ACGT | 9 cut(s) | 892, 967, 1275, 1812, 2336, 2425, 2539, 2603, 4210 |
| TaqII | GACCGA | 3 cut(s) | 2783, 4200, 4332 |
| TatI | WGTACW | 3 cut(s) | 922, 2380, 2510 |
| TauI | GCSGC | 4 cut(s) | 473, 690, 4112, 4296 |
| TscAI | CASTG | 5 cut(s) | 1223, 2157, 2954, 3499, 3985 |
| TseFI | GTSAC | 2 cut(s) | 1388, 3995 |
| TseI | GCWGC | 4 cut(s) | 2058, 2103, 2468, 3974 |
| Tsp45I | GTSAC | 2 cut(s) | 1388, 3995 |
| TspGWI | ACGGA | 4 cut(s) | 1638, 1988, 3588, 4555 |
| TspRI | CASTG | 5 cut(s) | 1223, 2157, 2954, 3499, 3985 |
| Vha464I | CTTAAG | 3 cut(s) | 478, 1937, 2042 |
| VpaK11BI | GGWCC | 2 cut(s) | 4, 4247 |
| VspI | ATTAAT | 1 cut(s) | 3033 |
| XapI | RAATTY | 6 cut(s) | 253, 729, 1864, 2372, 2925, 3299 |
| XbaI | TCTAGA | 2 cut(s) | 1324, 4417 |
| XceI | RCATGY | 5 cut(s) | 1016, 1104, 1338, 2239, 3220 |
| XhoI | CTCGAG | 1 cut(s) | 3203 |
| XmiI | GTMKAC | 2 cut(s) | 147, 1498 |
| XmnI | GAANNNNTTC | 3 cut(s) | 1076, 1091, 2211 |
| XspI | CTAG | 8 cut(s) | 905, 1002, 1298, 1325, 1424, 2055, 3486, 4418 |
| ZraI | GACGTC | 1 cut(s) | 4208 |
| Zsp2I | ATGCAT | 1 cut(s) | 3374 |