RchiOBHm_Chr1g0349181

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000067
Physical Location & Seq
Reverse (-)
42236351 .. 42241875
5525 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57516

Sequence Viewer

Length: 3375 bp
ATGATGAGGGAAAGACTAAGTAAGAAAAGAATTCTTCTTGTTCTTGATGATGTGGACAATCTGGACCAAATTGAAACCTTGATTGGAGAGAAACCTTCATTTGGTCACGGGAGCAGAATCATTATAACAACTAGAGATAAGAAGTTACTAGGTGGATGTAAGATATACGAGCCCGAGTTGTTGACTGATGAAGAAGCTCTCGAACTCTTTAGCAAGTATGCTTTCAGAACAAACAAACGCTCAGAGAATTACGATGCTATGTCAAGGTGTGCACTAAAATACGCCTATGGTCTTCCTTTGGCACTCAAAGTCTTGGGATGTTTGCTTGATAACAGGAGTGAACTGGAGTGGAAATTTGAGTTGGAGAAGATAAAGGAAATCCCAGTCACGTCTCTTGATGATAAAATTAGACGTGTGCTTTGGGCAAGCTATGATGGATTGGATGAATATCAGAAAAACATATTTCTAGATATTGCATGTTTCTTTAAGGGAAAGAACAAAGATTTTGTAACAAAATTTCATAAAAGTTGTGGCTATCACCCCCAGAATGGACTAACAGTTTTGGTAGAAAGATCTCTTGTAAGTGTCAATTTTGCTAATTATGATAGCCTAGAGATGCATGATTTGATACAGGAAGTGGGTAGGGACATTGTCCACCGACAATCTACTGAAGATCCGGGAATGCGTAGTAGATTATGGAGTTATGAAGATGTTCACCAAGTGTTAGCTCAAAATACGGCTACGAAAGTTGAAGGCATAATTCTAGACTTGTCAAAGTTCAAAGAGGTATACGTAAATGATGAAGTTTTTGTTAGCATGAGGAGACTAAGGCTACTCCAGCTTTATGAATATTATTATAAATATGAAGATGAATATGAATATGATTATGGAAGTGCAAGGAATGAATTTGCTTATAGGGAATTGACGCGACCTGCAGATTATGAACCTTGTGTACAGAAAGTGAGTGAGAACTTAAAGTTTCTTTCTCAAGAGTTGAGAATTCTTGTGTGGCATGGATGCCCACTAAAGTCTTTCCCAATTAATTTTCTACCAAAGAATCTTATTTATGTTGACATGTGCTATAGTCGTATTGAACAACTTTGGGAAGGAACCGGGCCTTTGCAACACTTGGTACACATCGATTTAAGATACTCAAAGTATCTAATCAAAACCCCCGATTTCACTGGGGCAACAAATCTTAAGAAATTGAAGCTTAAAGGATGTACCAGCTTATTGGAGGTTCACCCATCCATTTCAGCTCTTAAAAGCCTTGTTGAGTTGGACCTACGAGGCTGCTCAAATCTCAAAAAGTTTCCAAATATATCCGGAATTATGAAAAAGCTAGAAGCACTTTACTTAGATGAGACTGCAATTACAGAAATGCCCTTGTCAATTAATGATCTTTCCGGGTTGCAGACTTTGAGCCTCGCTGGTTGCAGAGAACTCAGGAGTCTTCCGAGCAGCATTCACATGAAATCTCTGAAATATCTTGTTCTATCTGGGTGCTCGAAACTTACGGAGTTTCCAGAGATTTCAGAAGTAATGAAGAAGCTATGGAAGCTTGAATTAGACGGGACAACAATTAAAGAACTACCCTCATCTATTAATAATCTTACGGGGCTTTGGGAGTTGAGTTTGACAAACTGCACAAGCCTTGTCTGTCTTCCGGACCATATCTGCAATTTGGCAAGCCTCAATCTGACTGGGTGCTCAAGCCTTGTCTGTCTTCCGGACAATATCTGCAATTTGGCAAGGCATTCTCATCTCAATCTGACTGGGTGCTCAAGCCTTGTCTGTCTTCCGGACAATATCTGCAATTTGGCATGCCTTTCTCATCTCAATCTGACTGGGTGCTCAAGCCTTGTCTGTCTTCCGGACAATATCTGCAATTTGGCAAGGCTTTCTCATCTCAATCTGACTGGGTGCTCAAGCCTTGTCTGTCTTCCGGACAATATCTTCAATTTGGCAAGGCTTTCTCAGCTCAATCTGACTGAGTGCTCTAACCTTTCAAACTGGCCTGAGTACATAGGGAATTTGAAACCCTTGTGGCAATTTGAAGTGGGGAGAAGTGGTATAAAACAACTTACATTCTCTATCTTGCGTTTAGGGGATTCATTATCATTATCATGTAATGGATGTAAAGAGATGACGGCGCCCTTTTCCTCGTTGCCAACATGGAACCACCAAAATCAGAGGAACTCTTTTCTGGAGAGTCTGGATTTAAGTGACTGCAATCTCTTGGAATTATCATATGGCATTGCTCATTTATTATCAGATGGCATTGCTCATTTATCATTGTATGAATTAAACTTATCCGGAAACAACAAGTTGGAAAGTTTACCTGCAGCAATGATTCGCCTGGGTCATTTAAGACGGCTCTATCTGAGAGGTTGCAAGAGACTGAAATCAATACCAGAGCTTTCATCGAGTATACAAGAAATCGATGCCCAAGATTGCGCATCTTTGGAGACAGTTTCAACACCACAGCCTCCGTACGCTGAGGACATTCGCTTCCGATTTGTTAATTGCTTCAAGTTGGTGACCAATACCAATCTATTTATAGATATTGTGGAAACTGCTACTGATATTCAGGTTATTTATGATCGTTATATTGGACCTCCTTACCATGTATCTCTTCCTGGAAGTGATATTCCAGAATGGTTCAACCATCAGAGTAGAGGGTCTTCCGTAACTGTGCAATTACCACCCAACTGGCTCGCAAACAAGTTTCTAGGGTTCGCTATATGCGCCATCGGTAACTTGCGGGGTGCTGCAAATACAAAAACTCAATCTGCTGTATGTTGTTGTACCTTAGAAGCAAATGATGGTCAGGACAATTTCTATTTTTCTTTGTTCGATATTCTAGATGGAACTGGCATGCCTCTTGAATCAGATCATATGTTGCTGGGATATACAAATTTACGAAGTTTAGGAGAGAGGCATAATGAACGCAAATACACCGAGGCCAGATTTAGGATAGTGGCAAGCACCTATGGAGTCATCCCAAATGATTCAATGACATTAGAAGATTGCACTTGGATCACAAGTTGCGGGGTTCGGTTATTCCACATAAATCCACCCTCGGTGGATCTTATTTGTAATTTGGAAAATAGAGAAACAGAGATAAGGAGTAAGAGAAAGAGAGAAACACTAGAGAGAGGGAAAGAGAAGATGGGGGTGGAGGTGCAGGAAGAGGGGGAGGAGTGCTCAGGAGGGCCCATATGCTGCTGCTTCTCGTTGAGTGGGTGTACAATATCGTTGAGAAAGCGACAAAAAGAAAAAGAAAGAGAGGGAGAGGGTGTTGCTGGAGACACAGGATTAGAGTCAGAGGACGCGGATGTCTCAGGGGAAGGGGGACTCCTCACATTATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1124

Amino Acids

126.86

Weight (kDa)

5.74

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 2 - 78 6.9e-11 NB-ARC domain
WHD_ROQ1 PF23282 149 - 222 2.4e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 413 - 489 2.2e-06 Leucine-rich repeat region
LRR_13 PF23286 421 - 505 3.9e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 492 - 573 7.6e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 493 - 566 1.4e-07 Leucine-rich repeat region
LRR_14 PF23598 565 - 666 7.2e-08 Leucine-rich repeat region
C-JID PF20160 879 - 1025 3.2e-21 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 125, 858
AasI GACNNNNNNGTC 1 cut(s) 3338
Acc16I TGCGCA 1 cut(s) 2458
Acc36I ACCTGC 2 cut(s) 940, 2350
AccB1I GGYRCC 1 cut(s) 2152
AccI GTMKAC 2 cut(s) 789, 2431
AccII CGCG 2 cut(s) 928, 3335
AccIII TCCGGA 7 cut(s) 1325, 1666, 1729, 1801, 1873, 1945, 2315
AciI CCGC 3 cut(s) 2764, 3051, 3335
AclWI GGATC 3 cut(s) 668, 3047, 3096
AcsI RAATTY 7 cut(s) 30, 353, 515, 905, 999, 2032, 2917
AcuI CTGAAG 1 cut(s) 690
AcyI GRCGYC 1 cut(s) 2153
AdeI CACNNNGTG 1 cut(s) 721
AfaI GTAC 7 cut(s) 954, 1134, 1225, 2024, 2495, 2809, 3250
AfiI CCNNNNNNNGG 3 cut(s) 101, 548, 2973
AflII CTTAAG 1 cut(s) 1199
AflIII ACRYGT 2 cut(s) 412, 1074
AjiI CACGTC 2 cut(s) 390, 413
AjnI CCWGG 2 cut(s) 2358, 2638
AjuI GAANNNNNNNTTGG 4 cut(s) 66, 98, 344, 376
Alw21I GWGCWC 8 cut(s) 274, 1508, 1712, 1784, 1856, 1928, 2000, 3209
Alw26I GTCTC 7 cut(s) 396, 817, 1358, 2392, 2462, 3303, 3346
Alw44I GTGCAC 1 cut(s) 270
AlwI GGATC 3 cut(s) 668, 3047, 3096
Ama87I CYCGRG 1 cut(s) 173
Aor13HI TCCGGA 7 cut(s) 1325, 1666, 1729, 1801, 1873, 1945, 2315
AoxI GGCC 4 cut(s) 1115, 2015, 2964, 3215
ApaI GGGCCC 1 cut(s) 3219
ApaLI GTGCAC 1 cut(s) 270
ApeKI GCWGC 6 cut(s) 1293, 1461, 2345, 2771, 3225, 3228
ApoI RAATTY 7 cut(s) 30, 353, 515, 905, 999, 2032, 2917
ArsI GACNNNNNNTTYG 2 cut(s) 402, 434
AseI ATTAAT 3 cut(s) 1041, 1395, 1605
Asp700I GAANNNNTTC 1 cut(s) 711
AspLEI GCGC 3 cut(s) 2155, 2459, 2750
AspS9I GGNCC 7 cut(s) 64, 1115, 1282, 1669, 2615, 3215, 3216
AsuC2I CCSGG 3 cut(s) 678, 1114, 1408
AsuHPI GGTGA 4 cut(s) 530, 707, 1235, 2551
AvaI CYCGRG 1 cut(s) 173
AvaII GGWCC 4 cut(s) 64, 1282, 1669, 2615
BaeGI GKGCMC 2 cut(s) 274, 3219
BanI GGYRCC 1 cut(s) 2152
BanII GRGCYC 2 cut(s) 174, 3219
BarI GAAGNNNNNNTAC 2 cut(s) 2668, 2700
BbsI GAAGAC 8 cut(s) 284, 1445, 1655, 1718, 1790, 1862, 1934, 2676
Bbv12I GWGCWC 8 cut(s) 274, 1508, 1712, 1784, 1856, 1928, 2000, 3209
BbvCI CCTCAGC 1 cut(s) 2499
BbvI GCAGC 6 cut(s) 1280, 1473, 2357, 2758, 3212, 3215
BccI CCATC 8 cut(s) 428, 1255, 2270, 2676, 2759, 2819, 2861, 3166
BceAI ACGGC 3 cut(s) 753, 2166, 2390
BciT130I CCWGG 2 cut(s) 2360, 2640
BcnI CCSGG 3 cut(s) 678, 1114, 1408
BcoDI GTCTC 7 cut(s) 396, 817, 1358, 2392, 2462, 3303, 3346
BfaI CTAG 9 cut(s) 132, 149, 467, 611, 764, 1343, 2732, 2864, 3152
BfmI CTRYAG 3 cut(s) 933, 1081, 2343
BfoI RGCGCY 1 cut(s) 2156
BfrI CTTAAG 1 cut(s) 1199
BfuAI ACCTGC 2 cut(s) 940, 2350
BglII AGATCT 1 cut(s) 572
BisI GCNGC 6 cut(s) 1294, 1462, 2346, 2772, 3226, 3229
BlsI GCNGC 6 cut(s) 1295, 1463, 2347, 2773, 3227, 3230
Bme1390I CCNGG 5 cut(s) 678, 1114, 1408, 2360, 2640
Bme18I GGWCC 4 cut(s) 64, 1282, 1669, 2615
BmeT110I CYCGRG 1 cut(s) 173
BmgBI CACGTC 2 cut(s) 390, 413
BmgT120I GGNCC 7 cut(s) 64, 1115, 1282, 1669, 2615, 3215, 3216
BmiI GGNNCC 4 cut(s) 1111, 2154, 2180, 3217
BmrFI CCNGG 5 cut(s) 678, 1114, 1408, 2360, 2640
BmrI ACTGGG 6 cut(s) 377, 1194, 1713, 1785, 1857, 1929
BmsI GCATC 5 cut(s) 244, 606, 1007, 2434, 2468
BmuI ACTGGG 6 cut(s) 377, 1194, 1713, 1785, 1857, 1929
BpiI GAAGAC 8 cut(s) 284, 1445, 1655, 1718, 1790, 1862, 1934, 2676
BplI GAGNNNNNCTC 2 cut(s) 3191, 3223
BpmI CTGGAG 4 cut(s) 365, 821, 2228, 3327
Bpu10I CCTNAGC 2 cut(s) 2499, 3208
BpuEI CTTGAG 5 cut(s) 972, 1696, 1768, 1840, 1912
BpuMI CCSGG 3 cut(s) 678, 1114, 1408
Bsa29I ATCGAT 2 cut(s) 1140, 2442
BsaAI YACGTR 1 cut(s) 793
BsaBI GATNNNNATC 1 cut(s) 447
BsaHI GRCGYC 1 cut(s) 2153
BsaJI CCNNGG 3 cut(s) 2359, 2961, 3081
BsaWI WCCGGW 7 cut(s) 1325, 1666, 1729, 1801, 1873, 1945, 2315
BsaXI ACNNNNNCTCC 4 cut(s) 2056, 2086, 3285, 3315
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 548, 2973
Bse3DI GCAATG 3 cut(s) 2256, 2280, 2355
Bse8I GATNNNNATC 1 cut(s) 447
BseAI TCCGGA 7 cut(s) 1325, 1666, 1729, 1801, 1873, 1945, 2315
BseBI CCWGG 2 cut(s) 2360, 2640
BseCI ATCGAT 2 cut(s) 1140, 2442
BseDI CCNNGG 3 cut(s) 2359, 2961, 3081
BseGI GGATG 9 cut(s) 161, 323, 448, 1022, 1226, 1247, 2141, 3000, 3343
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 3 cut(s) 101, 548, 2973
BseMI GCAATG 3 cut(s) 2256, 2280, 2355
BseMII CTCAG 9 cut(s) 255, 1459, 1983, 1991, 2010, 2375, 2490, 3222, 3357
BseRI GAGGAG 3 cut(s) 835, 3215, 3350
BseSI GKGCMC 2 cut(s) 274, 3219
BseXI GCAGC 6 cut(s) 1280, 1473, 2357, 2758, 3212, 3215
BseYI CCCAGC 1 cut(s) 2905
BsgI GTGCAG 2 cut(s) 1630, 3206
Bsh1236I CGCG 2 cut(s) 928, 3335
BshFI GGCC 4 cut(s) 1117, 2017, 2966, 3217
BshNI GGYRCC 1 cut(s) 2152
BshVI ATCGAT 2 cut(s) 1140, 2442
BsiHKAI GWGCWC 8 cut(s) 274, 1508, 1712, 1784, 1856, 1928, 2000, 3209
BsiHKCI CYCGRG 1 cut(s) 173
BsiWI CGTACG 1 cut(s) 2493
BslFI GGGAC 3 cut(s) 659, 1588, 3369
BslI CCNNNNNNNGG 3 cut(s) 101, 548, 2973
BsmAI GTCTC 7 cut(s) 396, 817, 1358, 2392, 2462, 3303, 3346
BsmBI CGTCTC 1 cut(s) 396
BsmFI GGGAC 3 cut(s) 659, 1588, 3369
BsmI GAATGC 3 cut(s) 687, 1464, 1756
BsnI GGCC 4 cut(s) 1117, 2017, 2966, 3217
BsoBI CYCGRG 1 cut(s) 173
Bsp120I GGGCCC 1 cut(s) 3215
Bsp13I TCCGGA 7 cut(s) 1325, 1666, 1729, 1801, 1873, 1945, 2315
Bsp1407I TGTACA 2 cut(s) 952, 3248
Bsp143I GATC 7 cut(s) 572, 673, 1399, 2602, 2893, 3039, 3088
BspACI CCGC 3 cut(s) 2764, 3051, 3335
BspANI GGCC 4 cut(s) 1117, 2017, 2966, 3217
BspCNI CTCAG 9 cut(s) 254, 1458, 1984, 1990, 2011, 2376, 2491, 3221, 3356
BspDI ATCGAT 2 cut(s) 1140, 2442
BspEI TCCGGA 7 cut(s) 1325, 1666, 1729, 1801, 1873, 1945, 2315
BspFNI CGCG 2 cut(s) 928, 3335
BspLI GGNNCC 4 cut(s) 1111, 2154, 2180, 3217
BspMAI CTGCAG 2 cut(s) 937, 2347
BspMI ACCTGC 2 cut(s) 940, 2350
BspPI GGATC 3 cut(s) 668, 3047, 3096
BspT107I GGYRCC 1 cut(s) 2152
BspTI CTTAAG 1 cut(s) 1199
BsrDI GCAATG 3 cut(s) 2256, 2280, 2355
BsrGI TGTACA 2 cut(s) 952, 3248
BssECI CCNNGG 3 cut(s) 2359, 2961, 3081
BssMI GATC 7 cut(s) 572, 673, 1399, 2602, 2893, 3039, 3088
BssNAI GTATAC 2 cut(s) 790, 2432
BssNI GRCGYC 1 cut(s) 2153
Bst1107I GTATAC 2 cut(s) 790, 2432
Bst2UI CCWGG 2 cut(s) 2360, 2640
Bst4CI ACNGT 3 cut(s) 559, 2473, 2695
Bst6I CTCTTC 2 cut(s) 2640, 3186
BstACI GRCGYC 1 cut(s) 2153
BstAFI CTTAAG 1 cut(s) 1199
BstAUI TGTACA 2 cut(s) 952, 3248
BstBAI YACGTR 1 cut(s) 793
BstC8I GCNNGC 6 cut(s) 427, 1690, 1825, 2718, 2879, 2986
BstEII GGTNACC 1 cut(s) 2539
BstF5I GGATG 9 cut(s) 161, 323, 448, 1022, 1226, 1247, 2141, 3000, 3343
BstFNI CGCG 2 cut(s) 928, 3335
BstH2I RGCGCY 1 cut(s) 2156
BstHHI GCGC 3 cut(s) 2155, 2459, 2750
BstKTI GATC 7 cut(s) 575, 676, 1402, 2605, 2896, 3042, 3091
BstMAI GTCTC 7 cut(s) 396, 817, 1358, 2392, 2462, 3303, 3346
BstMBI GATC 7 cut(s) 572, 673, 1399, 2602, 2893, 3039, 3088
BstMWI GCNNNNNNNGC 4 cut(s) 838, 1558, 1978, 2747
BstNI CCWGG 2 cut(s) 2360, 2640
BstNSI RCATGY 4 cut(s) 480, 1078, 1827, 2881
BstPI GGTNACC 1 cut(s) 2539
BstSCI CCNGG 5 cut(s) 676, 1112, 1406, 2358, 2638
BstSFI CTRYAG 3 cut(s) 933, 1081, 2343
BstSLI GKGCMC 2 cut(s) 274, 3219
BstSNI TACGTA 1 cut(s) 793
BstUI CGCG 2 cut(s) 928, 3335
BstV1I GCAGC 6 cut(s) 1280, 1473, 2357, 2758, 3212, 3215
BstV2I GAAGAC 8 cut(s) 284, 1445, 1655, 1718, 1790, 1862, 1934, 2676
BstX2I RGATCY 3 cut(s) 572, 673, 3088
BstXI CCANNNNNNTGG 2 cut(s) 1234, 2712
BstYI RGATCY 3 cut(s) 572, 673, 3088
BstZ17I GTATAC 2 cut(s) 790, 2432
Bsu15I ATCGAT 2 cut(s) 1140, 2442
BsuRI GGCC 4 cut(s) 1117, 2017, 2966, 3217
BsuTUI ATCGAT 2 cut(s) 1140, 2442
BtrI CACGTC 2 cut(s) 390, 413
BtsCI GGATG 9 cut(s) 161, 323, 448, 1022, 1226, 1247, 2141, 3000, 3343
BtsIMutI CAGTG 1 cut(s) 1182
BveI ACCTGC 2 cut(s) 940, 2350
Cac8I GCNNGC 6 cut(s) 427, 1690, 1825, 2718, 2879, 2986
CfoI GCGC 3 cut(s) 2155, 2459, 2750
Cfr13I GGNCC 7 cut(s) 64, 1115, 1282, 1669, 2615, 3215, 3216
ClaI ATCGAT 2 cut(s) 1140, 2442
CseI GACGC 2 cut(s) 934, 3341
Csp6I GTAC 7 cut(s) 953, 1133, 1224, 2023, 2494, 2808, 3249
CviQI GTAC 7 cut(s) 953, 1133, 1224, 2023, 2494, 2808, 3249
DinI GGCGCC 1 cut(s) 2154
DpnI GATC 7 cut(s) 574, 675, 1401, 2604, 2895, 3041, 3090
DpnII GATC 7 cut(s) 572, 673, 1399, 2602, 2893, 3039, 3088
DraIII CACNNNGTG 1 cut(s) 721
DrdI GACNNNNNNGTC 1 cut(s) 3338
DseDI GACNNNNNNGTC 1 cut(s) 3338
Eam1104I CTCTTC 2 cut(s) 2640, 3186
EarI CTCTTC 2 cut(s) 2640, 3186
Eco105I TACGTA 1 cut(s) 793
Eco24I GRGCYC 2 cut(s) 174, 3219
Eco47I GGWCC 4 cut(s) 64, 1282, 1669, 2615
Eco57I CTGAAG 1 cut(s) 690
Eco88I CYCGRG 1 cut(s) 173
Eco91I GGTNACC 1 cut(s) 2539
EcoO109I RGGNCCY 1 cut(s) 3215
EcoO65I GGTNACC 1 cut(s) 2539
EcoRI GAATTC 2 cut(s) 30, 999
EcoRII CCWGG 2 cut(s) 2358, 2638
EcoT22I ATGCAT 1 cut(s) 621
EcoT38I GRGCYC 2 cut(s) 174, 3219
EgeI GGCGCC 1 cut(s) 2154
EheI GGCGCC 1 cut(s) 2154
Esp3I CGTCTC 1 cut(s) 396
FaqI GGGAC 3 cut(s) 659, 1588, 3369
FauI CCCGC 2 cut(s) 2757, 3044
FauNDI CATATG 3 cut(s) 2251, 2898, 3221
FblI GTMKAC 2 cut(s) 789, 2431
Fnu4HI GCNGC 6 cut(s) 1294, 1462, 2346, 2772, 3226, 3229
FokI GGATG 9 cut(s) 168, 330, 455, 1029, 1233, 1234, 2148, 2987, 3350
FriOI GRGCYC 2 cut(s) 174, 3219
Fsp4HI GCNGC 6 cut(s) 1294, 1462, 2346, 2772, 3226, 3229
FspBI CTAG 9 cut(s) 132, 149, 467, 611, 764, 1343, 2732, 2864, 3152
FspI TGCGCA 1 cut(s) 2458
GlaI GCGC 3 cut(s) 2154, 2458, 2749
GluI GCNGC 6 cut(s) 1294, 1462, 2346, 2772, 3226, 3229
GsaI CCCAGC 1 cut(s) 2909
GsuI CTGGAG 4 cut(s) 365, 821, 2228, 3327
HaeII RGCGCY 1 cut(s) 2156
HaeIII GGCC 4 cut(s) 1117, 2017, 2966, 3217
HgaI GACGC 2 cut(s) 934, 3341
HhaI GCGC 3 cut(s) 2155, 2459, 2750
Hin1I GRCGYC 1 cut(s) 2153
Hin6I GCGC 3 cut(s) 2153, 2457, 2748
HinP1I GCGC 3 cut(s) 2153, 2457, 2748
HincII GTYRAC 2 cut(s) 183, 1072
HindII GTYRAC 2 cut(s) 183, 1072
HindIII AAGCTT 2 cut(s) 1211, 1559
HphI GGTGA 4 cut(s) 530, 707, 1235, 2551
HpyAV CCTTC 4 cut(s) 105, 746, 1100, 3344
HpyCH4III ACNGT 3 cut(s) 559, 2473, 2695
HpyCH4IV ACGT 3 cut(s) 389, 412, 792
HpyF10VI GCNNNNNNNGC 4 cut(s) 838, 1558, 1978, 2747
HpySE526I ACGT 3 cut(s) 389, 412, 792
Hsp92I GRCGYC 1 cut(s) 2153
HspAI GCGC 3 cut(s) 2153, 2457, 2748
KasI GGCGCC 1 cut(s) 2152
Kpn2I TCCGGA 7 cut(s) 1325, 1666, 1729, 1801, 1873, 1945, 2315
Kzo9I GATC 7 cut(s) 572, 673, 1399, 2602, 2893, 3039, 3088
LmnI GCTCC 1 cut(s) 111
Lsp1109I GCAGC 6 cut(s) 1280, 1473, 2357, 2758, 3212, 3215
LweI GCATC 5 cut(s) 244, 606, 1007, 2434, 2468
MaeI CTAG 9 cut(s) 132, 149, 467, 611, 764, 1343, 2732, 2864, 3152
MaeII ACGT 3 cut(s) 389, 412, 792
MaeIII GTNAC 8 cut(s) 104, 144, 385, 508, 2225, 2539, 2689, 2756
MalI GATC 7 cut(s) 574, 675, 1401, 2604, 2895, 3041, 3090
MboI GATC 7 cut(s) 572, 673, 1399, 2602, 2893, 3039, 3088
MflI RGATCY 3 cut(s) 572, 673, 3088
Mly113I GGCGCC 1 cut(s) 2153
MlyI GAGTC 5 cut(s) 1459, 2221, 3006, 3332, 3351
MmeI TCCRAC 3 cut(s) 342, 1260, 2310
Mph1103I ATGCAT 1 cut(s) 621
MroI TCCGGA 7 cut(s) 1325, 1666, 1729, 1801, 1873, 1945, 2315
MroXI GAANNNNTTC 1 cut(s) 711
MslI CAYNNNNRTG 2 cut(s) 1469, 2125
MspCI CTTAAG 1 cut(s) 1199
MspR9I CCNGG 5 cut(s) 678, 1114, 1408, 2360, 2640
Mva1269I GAATGC 3 cut(s) 687, 1464, 1756
MvaI CCWGG 2 cut(s) 2360, 2640
MvnI CGCG 2 cut(s) 928, 3335
MwoI GCNNNNNNNGC 4 cut(s) 838, 1558, 1978, 2747
NarI GGCGCC 1 cut(s) 2153
NciI CCSGG 3 cut(s) 678, 1114, 1408
NdeI CATATG 3 cut(s) 2251, 2898, 3221
NdeII GATC 7 cut(s) 572, 673, 1399, 2602, 2893, 3039, 3088
NlaIV GGNNCC 4 cut(s) 1111, 2154, 2180, 3217
NmuCI GTSAC 4 cut(s) 104, 385, 2225, 2539
NsbI TGCGCA 1 cut(s) 2458
NsiI ATGCAT 1 cut(s) 621
NspI RCATGY 4 cut(s) 480, 1078, 1827, 2881
PaeI GCATGC 2 cut(s) 1827, 2881
PciI ACATGT 1 cut(s) 1074
PctI GAATGC 3 cut(s) 687, 1464, 1756
PdmI GAANNNNTTC 1 cut(s) 711
PfeI GAWTC 6 cut(s) 117, 1057, 2111, 2353, 2888, 3011
Pfl23II CGTACG 1 cut(s) 2493
PflFI GACNNNGTC 1 cut(s) 650
PfoI TCCNGGA 2 cut(s) 676, 2638
PkrI GCNGC 6 cut(s) 1295, 1463, 2347, 2773, 3227, 3230
PleI GAGTC 5 cut(s) 1458, 2220, 3005, 3331, 3351
PluTI GGCGCC 1 cut(s) 2156
PpsI GAGTC 5 cut(s) 1458, 2220, 3005, 3331, 3351
Ppu21I YACGTR 1 cut(s) 793
PscI ACATGT 1 cut(s) 1074
PshBI ATTAAT 3 cut(s) 1041, 1395, 1605
PsiI TTATAA 2 cut(s) 125, 858
Psp6I CCWGG 2 cut(s) 2358, 2638
PspEI GGTNACC 1 cut(s) 2539
PspFI CCCAGC 1 cut(s) 2905
PspGI CCWGG 2 cut(s) 2358, 2638
PspLI CGTACG 1 cut(s) 2493
PspN4I GGNNCC 4 cut(s) 1111, 2154, 2180, 3217
PspOMI GGGCCC 1 cut(s) 3215
PspPI GGNCC 7 cut(s) 64, 1115, 1282, 1669, 2615, 3215, 3216
PsrI GAACNNNNNNTAC 2 cut(s) 936, 968
PstI CTGCAG 2 cut(s) 937, 2347
PsuI RGATCY 3 cut(s) 572, 673, 3088
PsyI GACNNNGTC 1 cut(s) 650
RsaI GTAC 7 cut(s) 954, 1134, 1225, 2024, 2495, 2809, 3250
RsaNI GTAC 7 cut(s) 953, 1133, 1224, 2023, 2494, 2808, 3249
RseI CAYNNNNRTG 2 cut(s) 1469, 2125
SatI GCNGC 6 cut(s) 1294, 1462, 2346, 2772, 3226, 3229
Sau3AI GATC 7 cut(s) 572, 673, 1399, 2602, 2893, 3039, 3088
Sau96I GGNCC 7 cut(s) 64, 1115, 1282, 1669, 2615, 3215, 3216
SchI GAGTC 5 cut(s) 1459, 2221, 3006, 3332, 3351
ScrFI CCNGG 5 cut(s) 678, 1114, 1408, 2360, 2640
SfaNI GCATC 5 cut(s) 244, 606, 1007, 2434, 2468
SfcI CTRYAG 3 cut(s) 933, 1081, 2343
SfoI GGCGCC 1 cut(s) 2154
SinI GGWCC 4 cut(s) 64, 1282, 1669, 2615
SmiMI CAYNNNNRTG 2 cut(s) 1469, 2125
SmlI CTYRAG 6 cut(s) 987, 1199, 1711, 1783, 1855, 1927
SmoI CTYRAG 6 cut(s) 987, 1199, 1711, 1783, 1855, 1927
SnaBI TACGTA 1 cut(s) 793
SphI GCATGC 2 cut(s) 1827, 2881
SsiI CCGC 3 cut(s) 2764, 3051, 3335
SspDI GGCGCC 1 cut(s) 2152
SspI AATATT 1 cut(s) 851
SspMI CTAG 9 cut(s) 132, 149, 467, 611, 764, 1343, 2732, 2864, 3152
StyD4I CCNGG 5 cut(s) 676, 1112, 1406, 2358, 2638
TaaI ACNGT 3 cut(s) 559, 2473, 2695
TaiI ACGT 3 cut(s) 392, 415, 795
TaqI TCGA 6 cut(s) 201, 1140, 1508, 2426, 2442, 2856
TatI WGTACW 3 cut(s) 952, 2022, 3248
TfiI GAWTC 6 cut(s) 117, 1057, 2111, 2353, 2888, 3011
TscAI CASTG 1 cut(s) 1189
TseFI GTSAC 4 cut(s) 104, 385, 2225, 2539
TseI GCWGC 6 cut(s) 1293, 1461, 2345, 2771, 3225, 3228
Tsp45I GTSAC 4 cut(s) 104, 385, 2225, 2539
TspGWI ACGGA 3 cut(s) 1532, 2481, 2677
TspRI CASTG 1 cut(s) 1189
Tth111I GACNNNGTC 1 cut(s) 650
Vha464I CTTAAG 1 cut(s) 1199
VneI GTGCAC 1 cut(s) 270
VpaK11BI GGWCC 4 cut(s) 64, 1282, 1669, 2615
VspI ATTAAT 3 cut(s) 1041, 1395, 1605
XapI RAATTY 7 cut(s) 30, 353, 515, 905, 999, 2032, 2917
XbaI TCTAGA 3 cut(s) 466, 763, 2863
XceI RCATGY 4 cut(s) 480, 1078, 1827, 2881
XmiI GTMKAC 2 cut(s) 789, 2431
XmnI GAANNNNTTC 1 cut(s) 711
XspI CTAG 9 cut(s) 132, 149, 467, 611, 764, 1343, 2732, 2864, 3152
Zsp2I ATGCAT 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.