Rw1G019150

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000067
Physical Location & Seq
Reverse (-)
41922650 .. 41929079
6430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G019150.1

Sequence Viewer

Length: 4470 bp
ATGGATTTTGCTTCCTCTTCTTCTTCTTCTTCTTCATCTTCATCTTCATTGACCTATGATGTCTTCCTCAGTTTTAGAGGCGAAGACACCCGACAGAGCTTTGTTTGCCATCTTCACAAAGCTCTGGAACTGAAGGCACTCAAAGTCTACATTGATAAAGAAGATCTTGAAAAAGGCAACCACCTTTCGGATCTACTGAAAGCCATTGCTGAATCAAAGCTTTCGATTGTAGTTTTCTCTGAGAACTACGGGAATTCCACATGGTGCTTGAAAGAACTGGTGAAGATCCTGGATTGCAATAAGGCGAAGCAGAAGCAGATTGTGATTCCCGTTTTCTACAAAGTAGATCCTTCCCACATTCGTAAACAGACGGGAAGGTTTGGCAAAGCTTATGACGAGCATGAGCACAAATGCAAAAAGGACAAGGAGTTGCTGCAAGAAGTGCAGAGATGGAGACCCGCTTTAAGCAAAGCCGCCAACTTCAGTGGCTGGGATTCCAATTCCAAAGATTTTGGGGATGATGCCAAGCTCATTGAGCGTATTGTAAATGATGTCCTTGATAAAGTGAATCGGATCTCGCCACACATAGAGGATGGCTTGGTTGCAATGGATTCCCATCTGCATCAAGTGGGACGACTATTAAATGATGCGCCGGATGATGTTTGTTATGTTGGAATCTGGGGAATGGGCGGCTTGGGTAAAACAACAATTGCCAGGGCCGTTTATGACAGAATTTGTCATAAATTTGACCACCGTTGCTTTCTTCAAAATGTGAGGGAACGCTTCACGAGGAACGATGGAGTACAAATGAAAGTTGAATTTCTATCTGGAATCTTGAACGAAAAAGTCAGCAATTTTGATGGAGGTTACGAGAAGAGGCTGAAAAGGCTAGGTGAAAAAAAAATCCTAGTTGTTCTTGACGATGTACTCAACTTAAACCAGATTGATACCTTGCTCGGAATGAAACGTTCATTTGGTGGTGGAAGTATAATTATTGTAACAACTAGAGATGAACATGTGGTGCGTGGATTTAAGATATACAAGCCCAAGCCATTAAGTGATGAAGATGCTCTGAAACTCTTCAGCCAGAAGGCCTTCAACACATACAAACCCCCTGGAGAGTATGAACATCACTCAAAGTGCGTAGTACAATATGCACACGGTCTGCCCTTAGCACTTAAAGTATTGGGAGCGTTTTTTCGTGACAGAAGTGTATCTCAGTGGGAAGGTCAGTTGAACAAATTGAAGAAATTGAAGAAATTGAAGGACCCACAAATTAAGGAAGTGCTTAAAATAAGCTATGATGGACTAGATCCTTCCCAGCAGGACATATTTCTAGATATTGCATGTTTCTTTAAAGGAATGGACAGAGACTACGCAACAGAGGTTCTTGTCAATTGTGACTCAACTCCCGACATTGACTTGGATGTTCTAGTTGAAAGAGCTCTTGTAACAATCTCATACTACAATAAACTTGAGATGCATGATTTACTACAAGAAATGGGTATGGACATTGAATCCACTAAAGACGGGAAGCGGAGTAGATTTTGGGGTGAAGAAGCTGAGCGTGTGTTTACTGAAAATACGGCTACGGAATATGCGGAGCGCTTGATGATTGACTTGCCGTACAAGAAAGACGGATCCTTCAATACTTCAATGCGCATATTCACTTACGGGAAAAGGTATGCTGGAGATGACGGTGAAAATCACTTGATTGGGGACTACAAGTTTCTTTCTCGTGAGTTGAAGGTTCTCGTCTGGCATGGATATCCCCATAAGTGTTTGCCATCCAACTTTGACCCCAAGAGTCTTGTTCAGCTTGACCTGCGTTGCAGTAACATTGAACAACTTTGGGAAGGATCCAAGCCGGCGAAAAATTTGATAAGCATCGATTTGAGTGCATCTCCATATCTTAAAAGAACCCCTGACTTGACTCTTGCCACAAAGCTTAAGGAACTCCATTTTAATGGTTGTTCAACTTTAACGGAGGTTCACCCATCCATTTCAGCTCTTAAAAACCTTGTTTCCTTGAGGTTAGAAGCGTGCTATGAACTTAAGAGTCTTCCTAGCAGCATTGATATGAAATCTCTCAAAACCCTTAGTCTTTGGAGCTGCTCAAATCTTGAGATGTTTCCAGAGGTTTCGGAGAATATGGGAGCACTATCAGAGATTGTTTTAAAAGGCACTGCAATTAAGGAACTACCATGGTCAATTGACCGGCTTCGGGGGCTTAAAAAATTAAGCATGTCATCCTGTCGTAGCCTTCCAGACGGTATTTGTAATTTAGCAGAACTTGAATCTCTCGATCTGTATGGGTGCTCAGAACTTCGTAATTTGCCTGAGAACATTGGGAATTTAGAATCTTTGTTGGAACTTGACGTACGCTATAGTGGTTTAGAACAACTACCTGTCTCTATATTACGTTTGAAACACGGTCGATTATTCTTTTATGGTTGCAGACAAATGGCGGCTCCCCTTTCATCATGGCCATCATCAATTGAAGATCGTTGTACTGTTGTGGTACATCTTGATTTAAGTGATTGCAATATGATGGAATTATCAGATGCTATTGCTTATTTTCCTTCATTGAAAGTATTAAGGTTGTCAAGAAACAATTTGGAGAGCTTACCTGCAATCATGAATGGACTTGCTTCCTTAGAACAGCTTGAATTGGAAGGTTGCAAGAGACTGAGATCAATACCAGAGCTTCCATCGACAATAAGTTACATAAATGCCCATGACTGCATAGCTTTGGAAACTGTTTCGACACCACAGTCTCCCTACGATATCGGTCGGTGCTTCATACTTTCTAATTGCAGTCAGCTGGTACAGGAAGATGTTTTCAGAGATATTGTAGAAACTCATTTCCTTTCTCAGGGTAATTGTTCCCGGCCCTTGTATGTCTCTTTGCCTGGAAGTGAAATTCCAGAGTGGTTCACCCATCAGTGTAGGGGGTCTTCTGTAACTGCCAAGCTACCACCAAATTGGTTTGACAACAAGTTTTTGGGCTTTACTATATGCGCGATTATTAATAAGCCCCAGGAGATCCCTGAGTGTACCGCTTTTTCGCCACAACCTTATTTTACACTGACTGCTCGATGTTTCTGTACCTTCAAAGGAGATCTTTGCGAGTACCGTTTCAGTTTCTATCTCTTTGATATTGTTTCTGGCGGTGGAAGTTGTTGGCCCGAGTCAAATCACATGTTAGTGGGATATGTGCCATGGTCTGAATCTGGCATGATTAAGAGAGAGGAAGTCAATGAACGCCGTTACACAGAGGCCAAATTTGAGATACAATTGCAGTATTCCTCGAATAGACACCGTGCCTATCTCAAATTGAGTGATGGATTCTACCCATCCATCGAAAGGTGCGGAGTTCGATTGGCGTTTGCCACCAATGAGGAAGATTCTAGGGAACCAATGGTAGAAGTTGATAATTCTGGGAGGCGCCTTGAGGAGTGCTTGGCTGAACCCTGTGGCAGTGACATCACATGTGACACCTCCGATGAGGATGAGCAATCCCTTAAATTATTATCAGAAGTTTTTAAAGGCGCAAACAGATCACCGAGTTACATCGAAATTGTGGACAACGATTCATCCAAGAAGAGGATAACTCTGGAGAGTTGGACGCAAAAAGTCAGAGTTACATCGGGTCTAAGGCAAGGGCTTCTGGATGATGGTTTTAACTGGTTCGGACGCACCTATGGATTCGAGAGAGACGAGTATTATTGTGCTCATAGTGGGACCCATCATAGCGGTTGGAAATATCATCAGCCTGACGACTGTAGGGCGAAAGCTATAGTGCGAACTCACATTGACCCAACAATCCTTGAAGTTAATTACATCGTGAGCCTACACACTTGTTGGAGATTTCAACCAATGTTGACACACCGAGTCAGAGTTACACCGGGTATGGGGACTGAAGGGCCTTTTGATGGCTTTACATGGGGGGAGTATGACCAGATGGACATACCAGGAGCTAAATACCCAAGAGTTTATTACATATGCACTCCTCAACATTATGGAGGTTGCATGGCCATAAAGGAAGTGCAGCTCTCAAATGATCAGAAAATCCTTAAAATTACTTACAGAGGAAGGCACACATGTACACTACCCTCCCCCGACATTGGAGGGAGGACCGATAGCAGTACCTCTGTGGCCAGTGGTGCATCAAGTGTTCATGACCAAGTTGTCGAATTACCAAGCTCACCAGGAGCCAATCGCGATGGAGGAAATGTAAACTCCCAGGATATGGAACACTTTGACAATGCCAATTATGGTGGTGCGGTGAGTGAGGGAATCAGTGATGCTGTGGTAAACCAGCAGAGGGTTTCTTTCGGGAAGAGGACCTTAGGGACTTTGAAGAGTCTCTGGACTTCAATAGCGTCAAGAAAAAGGACCCGAGAAATGATGGAAGCTGATGATGATCAACCAAATGAATCCAACTCCATTCCAAACAATAATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1489

Amino Acids

168.51

Weight (kDa)

6.03

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 19 - 197 3.8e-51 TIR domain
TIR_2 PF13676 21 - 117 3.3e-16 TIR domain
NB-ARC PF00931 210 - 367 2.7e-17 NB-ARC domain
WHD_ROQ1 PF23282 441 - 505 1.8e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 662 - 773 6.1e-10 Leucine-rich repeat region
LRR_13 PF23286 695 - 777 8e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 777 - 924 9e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_8 PF13855 842 - 894 3.1e-06 Leucine rich repeat
LRR_4 PF12799 861 - 904 3.5e-06 Leucine Rich repeats (2 copies)
C-JID PF20160 970 - 1132 3.3e-19 C-JID domain
WRKY PF03106 1312 - 1369 3.6e-15 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 2773, 4190
Acc16I TGCGCA 1 cut(s) 1661
Acc36I ACCTGC 2 cut(s) 1833, 2638
AccB1I GGYRCC 1 cut(s) 3447
AccB7I CCANNNNNTGG 1 cut(s) 4252
AccI GTMKAC 1 cut(s) 147
AccII CGCG 2 cut(s) 3023, 4224
AclI AACGTT 1 cut(s) 967
AcoI YGGCCR 3 cut(s) 2486, 4035, 4158
AcsI RAATTY 8 cut(s) 253, 732, 743, 818, 1876, 2353, 2922, 3284
AcuI CTGAAG 4 cut(s) 152, 466, 1066, 3942
AcyI GRCGYC 1 cut(s) 3448
AdeI CACNNNGTG 1 cut(s) 264
AfeI AGCGCT 1 cut(s) 1607
AflII CTTAAG 2 cut(s) 1949, 2054
AflIII ACRYGT 4 cut(s) 1015, 3201, 3491, 4103
AjnI CCWGG 8 cut(s) 288, 713, 1114, 2911, 3039, 3973, 4210, 4245
Alw21I GWGCWC 5 cut(s) 408, 1447, 2161, 2321, 3736
Alw26I GTCTC 8 cut(s) 448, 1365, 2416, 2680, 2781, 2908, 3711, 4373
AlwNI CAGNNNCTG 1 cut(s) 489
Ama87I CYCGRG 2 cut(s) 3188, 4401
Aor51HI AGCGCT 1 cut(s) 1607
AoxI GGCC 9 cut(s) 717, 1092, 2486, 2891, 3185, 3279, 3926, 4035, 4158
ApeKI GCWGC 4 cut(s) 433, 2070, 2112, 4051
ApoI RAATTY 8 cut(s) 253, 732, 743, 818, 1876, 2353, 2922, 3284
AseI ATTAAT 1 cut(s) 3030
Asp700I GAANNNNTTC 2 cut(s) 1079, 1094
AspLEI GCGC 6 cut(s) 652, 1608, 1662, 3023, 3450, 3554
AspS9I GGNCC 9 cut(s) 717, 1267, 2892, 3186, 3744, 3926, 4137, 4347, 4398
AsuC2I CCSGG 2 cut(s) 2890, 3909
AsuHPI GGTGA 9 cut(s) 292, 905, 1565, 1712, 1985, 2929, 3555, 4200, 4300
AvaI CYCGRG 2 cut(s) 3188, 4401
AvaII GGWCC 5 cut(s) 1267, 3744, 4137, 4347, 4398
AxyI CCTNAGG 1 cut(s) 4351
BalI TGGCCA 3 cut(s) 2488, 4037, 4160
BamHI GGATCC 2 cut(s) 1640, 1859
BanI GGYRCC 1 cut(s) 3447
BanII GRGCYC 1 cut(s) 1447
BarI GAAGNNNNNNTAC 2 cut(s) 2941, 2973
BauI CACGAG 2 cut(s) 787, 1737
BbsI GAAGAC 4 cut(s) 55, 90, 2054, 2949
Bbv12I GWGCWC 5 cut(s) 408, 1447, 2161, 2321, 3736
BbvI GCAGC 4 cut(s) 420, 2082, 2099, 4063
BceAI ACGGC 4 cut(s) 704, 1602, 1609, 3252
BcgI CGANNNNNNTGC 2 cut(s) 1880, 1914
BciT130I CCWGG 8 cut(s) 290, 715, 1116, 2913, 3041, 3975, 4212, 4247
BclI TGATCA 2 cut(s) 4063, 4426
BcnI CCSGG 2 cut(s) 2890, 3909
BcoDI GTCTC 8 cut(s) 448, 1365, 2416, 2680, 2781, 2908, 3711, 4373
BfaI CTAG 8 cut(s) 890, 908, 1005, 1310, 1337, 1433, 2067, 3411
BfmI CTRYAG 3 cut(s) 2386, 3784, 3798
BfoI RGCGCY 2 cut(s) 1609, 3451
BfrI CTTAAG 2 cut(s) 1949, 2054
BfuAI ACCTGC 2 cut(s) 1833, 2638
BglII AGATCT 2 cut(s) 163, 3121
BisI GCNGC 7 cut(s) 434, 474, 691, 2071, 2113, 2469, 4052
BlpI GCTNAGC 1 cut(s) 1563
BlsI GCNGC 7 cut(s) 435, 475, 692, 2072, 2114, 2470, 4053
Bme18I GGWCC 5 cut(s) 1267, 3744, 4137, 4347, 4398
BmeT110I CYCGRG 2 cut(s) 3188, 4401
BmgT120I GGNCC 9 cut(s) 717, 1267, 2892, 3186, 3744, 3926, 4137, 4347, 4398
BpiI GAAGAC 4 cut(s) 55, 90, 2054, 2949
BplI GAGNNNNNCTC 4 cut(s) 1888, 1920, 3598, 3630
BpmI CTGGAG 3 cut(s) 1137, 1710, 3638
Bpu10I CCTNAGC 1 cut(s) 1171
Bpu1102I GCTNAGC 1 cut(s) 1563
BpuEI CTTGAG 4 cut(s) 1496, 2050, 2144, 3473
BpuMI CCSGG 2 cut(s) 2890, 3909
Bsa29I ATCGAT 1 cut(s) 1890
BsaBI GATNNNNATC 3 cut(s) 615, 1886, 4425
BsaHI GRCGYC 1 cut(s) 3448
BsaI GGTCTC 1 cut(s) 448
BsaJI CCNNGG 6 cut(s) 714, 1114, 2204, 3039, 3221, 4245
BsaXI ACNNNNNCTCC 2 cut(s) 4221, 4251
Bse118I RCCGGY 2 cut(s) 1867, 2217
Bse1I ACTGG 3 cut(s) 282, 3692, 4161
Bse21I CCTNAGG 1 cut(s) 4351
Bse3DI GCAATG 2 cut(s) 204, 612
Bse8I GATNNNNATC 3 cut(s) 615, 1886, 4425
BseBI CCWGG 8 cut(s) 290, 715, 1116, 2913, 3041, 3975, 4212, 4247
BseCI ATCGAT 1 cut(s) 1890
BseDI CCNNGG 6 cut(s) 714, 1114, 2204, 3039, 3221, 4245
BseJI GATNNNNATC 3 cut(s) 615, 1886, 4425
BseMI GCAATG 2 cut(s) 204, 612
BseMII CTCAG 9 cut(s) 82, 231, 1232, 1554, 2331, 2334, 2681, 2888, 3042
BseNI ACTGG 3 cut(s) 282, 3692, 4161
BseRI GAGGAG 2 cut(s) 3470, 4002
BseXI GCAGC 4 cut(s) 420, 2082, 2099, 4063
BseYI CCCAGC 2 cut(s) 489, 1320
BsgI GTGCAG 2 cut(s) 464, 4070
Bsh1236I CGCG 2 cut(s) 3023, 4224
Bsh1285I CGRYCG 2 cut(s) 2437, 2794
BshFI GGCC 9 cut(s) 719, 1094, 2488, 2893, 3187, 3281, 3928, 4037, 4160
BshNI GGYRCC 1 cut(s) 3447
BshVI ATCGAT 1 cut(s) 1890
BsiEI CGRYCG 2 cut(s) 2437, 2794
BsiHKAI GWGCWC 5 cut(s) 408, 1447, 2161, 2321, 3736
BsiHKCI CYCGRG 2 cut(s) 3188, 4401
BsiSI CCGG 5 cut(s) 653, 1868, 2218, 2890, 3908
BsiWI CGTACG 1 cut(s) 2380
BslFI GGGAC 5 cut(s) 645, 1733, 3757, 3931, 4369
BsmAI GTCTC 8 cut(s) 448, 1365, 2416, 2680, 2781, 2908, 3711, 4373
BsmBI CGTCTC 1 cut(s) 3711
BsmFI GGGAC 5 cut(s) 645, 1733, 3757, 3931, 4369
BsnI GGCC 9 cut(s) 719, 1094, 2488, 2893, 3187, 3281, 3928, 4037, 4160
Bso31I GGTCTC 1 cut(s) 448
BsoBI CYCGRG 2 cut(s) 3188, 4401
Bsp1286I GDGCHC 5 cut(s) 408, 1447, 2161, 2321, 3736
Bsp1407I TGTACA 1 cut(s) 4106
Bsp1720I GCTNAGC 1 cut(s) 1563
Bsp19I CCATGG 2 cut(s) 2204, 3221
Bsp68I TCGCGA 1 cut(s) 4224
BspANI GGCC 9 cut(s) 719, 1094, 2488, 2893, 3187, 3281, 3928, 4037, 4160
BspCNI CTCAG 9 cut(s) 81, 232, 1231, 1555, 2332, 2333, 2682, 2887, 3043
BspDI ATCGAT 1 cut(s) 1890
BspFNI CGCG 2 cut(s) 3023, 4224
BspHI TCATGA 2 cut(s) 2637, 4180
BspMI ACCTGC 2 cut(s) 1833, 2638
BspT107I GGYRCC 1 cut(s) 3447
BspTI CTTAAG 2 cut(s) 1949, 2054
BspTNI GGTCTC 1 cut(s) 448
BsrDI GCAATG 2 cut(s) 204, 612
BsrFI RCCGGY 2 cut(s) 1867, 2217
BsrGI TGTACA 1 cut(s) 4106
BsrI ACTGG 3 cut(s) 282, 3692, 4161
BssAI RCCGGY 2 cut(s) 1867, 2217
BssECI CCNNGG 6 cut(s) 714, 1114, 2204, 3039, 3221, 4245
BssNI GRCGYC 1 cut(s) 3448
BssSI CACGAG 2 cut(s) 787, 1737
BssT1I CCWWGG 2 cut(s) 2204, 3221
Bst2BI CACGAG 2 cut(s) 787, 1737
Bst2UI CCWGG 8 cut(s) 290, 715, 1116, 2913, 3041, 3975, 4212, 4247
Bst6I CTCTTC 6 cut(s) 22, 869, 1085, 3599, 4337, 4358
BstACI GRCGYC 1 cut(s) 3448
BstAFI CTTAAG 2 cut(s) 1949, 2054
BstAPI GCANNNNNTGC 1 cut(s) 442
BstAUI TGTACA 1 cut(s) 4106
BstC8I GCNNGC 2 cut(s) 1869, 2044
BstDSI CCRYGG 2 cut(s) 2204, 3221
BstENI CCTNNNNNAGG 1 cut(s) 2873
BstFNI CGCG 2 cut(s) 3023, 4224
BstH2I RGCGCY 2 cut(s) 1609, 3451
BstHHI GCGC 6 cut(s) 652, 1608, 1662, 3023, 3450, 3554
BstMAI GTCTC 8 cut(s) 448, 1365, 2416, 2680, 2781, 2908, 3711, 4373
BstMCI CGRYCG 2 cut(s) 2437, 2794
BstMWI GCNNNNNNNGC 7 cut(s) 105, 442, 535, 886, 1825, 2227, 4166
BstNI CCWGG 8 cut(s) 290, 715, 1116, 2913, 3041, 3975, 4212, 4247
BstNSI RCATGY 6 cut(s) 1019, 1350, 2248, 3205, 3495, 4107
BstSFI CTRYAG 3 cut(s) 2386, 3784, 3798
BstUI CGCG 2 cut(s) 3023, 4224
BstV1I GCAGC 4 cut(s) 420, 2082, 2099, 4063
BstV2I GAAGAC 4 cut(s) 55, 90, 2054, 2949
BstXI CCANNNNNNTGG 2 cut(s) 1967, 2985
Bsu15I ATCGAT 1 cut(s) 1890
Bsu36I CCTNAGG 1 cut(s) 4351
BsuRI GGCC 9 cut(s) 719, 1094, 2488, 2893, 3187, 3281, 3928, 4037, 4160
BsuTUI ATCGAT 1 cut(s) 1890
BtgI CCRYGG 2 cut(s) 2204, 3221
BtgZI GCGATG 1 cut(s) 4239
BtsI GCAGTG 2 cut(s) 2184, 3487
BtsIMutI CAGTG 8 cut(s) 490, 1226, 2184, 2951, 3086, 3487, 4168, 4309
BtuMI TCGCGA 1 cut(s) 4224
BveI ACCTGC 2 cut(s) 1833, 2638
Cac8I GCNNGC 2 cut(s) 1869, 2044
CaiI CAGNNNCTG 1 cut(s) 489
CciI TCATGA 2 cut(s) 2637, 4180
CfoI GCGC 6 cut(s) 652, 1608, 1662, 3023, 3450, 3554
Cfr10I RCCGGY 2 cut(s) 1867, 2217
Cfr13I GGNCC 9 cut(s) 717, 1267, 2892, 3186, 3744, 3926, 4137, 4347, 4398
ClaI ATCGAT 1 cut(s) 1890
CseI GACGC 3 cut(s) 3637, 3705, 4374
CspCI CAANNNNNGTGG 4 cut(s) 466, 501, 4261, 4296
DinI GGCGCC 1 cut(s) 3449
DraI TTTAAA 3 cut(s) 1357, 2178, 3547
DraIII CACNNNGTG 1 cut(s) 264
DrdI GACNNNNNNGTC 2 cut(s) 2773, 4190
DseDI GACNNNNNNGTC 2 cut(s) 2773, 4190
EaeI YGGCCR 3 cut(s) 2486, 4035, 4158
Eam1104I CTCTTC 6 cut(s) 22, 869, 1085, 3599, 4337, 4358
EarI CTCTTC 6 cut(s) 22, 869, 1085, 3599, 4337, 4358
Ecl136II GAGCTC 1 cut(s) 1445
Eco130I CCWWGG 2 cut(s) 2204, 3221
Eco147I AGGCCT 1 cut(s) 1094
Eco24I GRGCYC 1 cut(s) 1447
Eco31I GGTCTC 1 cut(s) 448
Eco32I GATATC 2 cut(s) 1769, 2788
Eco47I GGWCC 5 cut(s) 1267, 3744, 4137, 4347, 4398
Eco47III AGCGCT 1 cut(s) 1607
Eco53kI GAGCTC 1 cut(s) 1445
Eco57I CTGAAG 4 cut(s) 152, 466, 1066, 3942
Eco81I CCTNAGG 1 cut(s) 4351
Eco88I CYCGRG 2 cut(s) 3188, 4401
EcoICRI GAGCTC 1 cut(s) 1445
EcoNI CCTNNNNNAGG 1 cut(s) 2873
EcoO109I RGGNCCY 5 cut(s) 1267, 3744, 3926, 4347, 4398
EcoRI GAATTC 1 cut(s) 253
EcoRII CCWGG 8 cut(s) 288, 713, 1114, 2911, 3039, 3973, 4210, 4245
EcoRV GATATC 2 cut(s) 1769, 2788
EcoT14I CCWWGG 2 cut(s) 2204, 3221
EcoT22I ATGCAT 1 cut(s) 1485
EcoT38I GRGCYC 1 cut(s) 1447
EgeI GGCGCC 1 cut(s) 3449
EheI GGCGCC 1 cut(s) 3449
ErhI CCWWGG 2 cut(s) 2204, 3221
Esp3I CGTCTC 1 cut(s) 3711
FalI AAGNNNNNCTT 6 cut(s) 150, 182, 3108, 3140, 4334, 4366
FaqI GGGAC 5 cut(s) 645, 1733, 3757, 3931, 4369
FauI CCCGC 1 cut(s) 466
FauNDI CATATG 1 cut(s) 4004
FbaI TGATCA 2 cut(s) 4063, 4426
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 7 cut(s) 434, 474, 691, 2071, 2113, 2469, 4052
FriOI GRGCYC 1 cut(s) 1447
Fsp4HI GCNGC 7 cut(s) 434, 474, 691, 2071, 2113, 2469, 4052
FspAI RTGCGCAY 1 cut(s) 1661
FspBI CTAG 8 cut(s) 890, 908, 1005, 1310, 1337, 1433, 2067, 3411
FspI TGCGCA 1 cut(s) 1661
GlaI GCGC 6 cut(s) 651, 1607, 1661, 3022, 3449, 3553
GluI GCNGC 7 cut(s) 434, 474, 691, 2071, 2113, 2469, 4052
GsaI CCCAGC 2 cut(s) 493, 1324
GsuI CTGGAG 3 cut(s) 1137, 1710, 3638
HaeII RGCGCY 2 cut(s) 1609, 3451
HaeIII GGCC 9 cut(s) 719, 1094, 2488, 2893, 3187, 3281, 3928, 4037, 4160
HapII CCGG 5 cut(s) 653, 1868, 2218, 2890, 3908
HgaI GACGC 3 cut(s) 3637, 3705, 4374
HhaI GCGC 6 cut(s) 652, 1608, 1662, 3023, 3450, 3554
Hin1I GRCGYC 1 cut(s) 3448
Hin6I GCGC 6 cut(s) 650, 1606, 1660, 3021, 3448, 3552
HinP1I GCGC 6 cut(s) 650, 1606, 1660, 3021, 3448, 3552
HincII GTYRAC 1 cut(s) 3885
HindII GTYRAC 1 cut(s) 3885
HindIII AAGCTT 3 cut(s) 218, 387, 1946
HpaII CCGG 5 cut(s) 653, 1868, 2218, 2890, 3908
HphI GGTGA 9 cut(s) 292, 905, 1565, 1712, 1985, 2929, 3555, 4200, 4300
HpyCH4IV ACGT 3 cut(s) 967, 2379, 2422
HpyF10VI GCNNNNNNNGC 7 cut(s) 105, 442, 535, 886, 1825, 2227, 4166
HpySE526I ACGT 3 cut(s) 967, 2379, 2422
Hsp92I GRCGYC 1 cut(s) 3448
HspAI GCGC 6 cut(s) 650, 1606, 1660, 3021, 3448, 3552
KasI GGCGCC 1 cut(s) 3447
KflI GGGWCCC 1 cut(s) 3744
KroI GCCGGC 1 cut(s) 1867
KroNI GCCGGC 1 cut(s) 1869
Ksp22I TGATCA 2 cut(s) 4063, 4426
LmnI GCTCC 7 cut(s) 1190, 1603, 2109, 2156, 2476, 3977, 4214
Lsp1109I GCAGC 4 cut(s) 420, 2082, 2099, 4063
MaeI CTAG 8 cut(s) 890, 908, 1005, 1310, 1337, 1433, 2067, 3411
MaeII ACGT 3 cut(s) 967, 2379, 2422
MfeI CAATTG 5 cut(s) 708, 1396, 2211, 2496, 3296
MhlI GDGCHC 5 cut(s) 408, 1447, 2161, 2321, 3736
MlsI TGGCCA 3 cut(s) 2488, 4037, 4160
MluNI TGGCCA 3 cut(s) 2488, 4037, 4160
Mly113I GGCGCC 1 cut(s) 3448
MlyI GAGTC 7 cut(s) 1397, 1816, 1927, 2068, 3200, 3903, 4375
MmeI TCCRAC 7 cut(s) 652, 1815, 2349, 3605, 3740, 3845, 4467
Mox20I TGGCCA 3 cut(s) 2488, 4037, 4160
Mph1103I ATGCAT 1 cut(s) 1485
MroNI GCCGGC 1 cut(s) 1867
MroXI GAANNNNTTC 2 cut(s) 1079, 1094
MscI TGGCCA 3 cut(s) 2488, 4037, 4160
MslI CAYNNNNRTG 3 cut(s) 1965, 2078, 3206
Msp20I TGGCCA 3 cut(s) 2488, 4037, 4160
MspA1I CMGCKG 1 cut(s) 2824
MspCI CTTAAG 2 cut(s) 1949, 2054
MspI CCGG 5 cut(s) 653, 1868, 2218, 2890, 3908
MunI CAATTG 5 cut(s) 708, 1396, 2211, 2496, 3296
MvaI CCWGG 8 cut(s) 290, 715, 1116, 2913, 3041, 3975, 4212, 4247
MvnI CGCG 2 cut(s) 3023, 4224
MwoI GCNNNNNNNGC 7 cut(s) 105, 442, 535, 886, 1825, 2227, 4166
NaeI GCCGGC 1 cut(s) 1869
NarI GGCGCC 1 cut(s) 3448
NciI CCSGG 2 cut(s) 2890, 3909
NcoI CCATGG 2 cut(s) 2204, 3221
NdeI CATATG 1 cut(s) 4004
NgoMIV GCCGGC 1 cut(s) 1867
NmuCI GTSAC 4 cut(s) 1202, 1400, 3482, 3494
NruI TCGCGA 1 cut(s) 4224
NsbI TGCGCA 1 cut(s) 1661
NsiI ATGCAT 1 cut(s) 1485
NspI RCATGY 6 cut(s) 1019, 1350, 2248, 3205, 3495, 4107
PagI TCATGA 2 cut(s) 2637, 4180
PceI AGGCCT 1 cut(s) 1094
PciI ACATGT 4 cut(s) 1015, 3201, 3491, 4103
PcsI WCGNNNNNNNCGW 1 cut(s) 3717
PdiI GCCGGC 1 cut(s) 1869
PdmI GAANNNNTTC 2 cut(s) 1079, 1094
Pfl23II CGTACG 1 cut(s) 2380
PflMI CCANNNNNTGG 1 cut(s) 4252
PfoI TCCNGGA 1 cut(s) 288
PkrI GCNGC 7 cut(s) 435, 475, 692, 2072, 2114, 2470, 4053
PleI GAGTC 7 cut(s) 1397, 1815, 1927, 2067, 3199, 3902, 4374
PluTI GGCGCC 1 cut(s) 3451
PpsI GAGTC 7 cut(s) 1397, 1815, 1927, 2067, 3199, 3902, 4374
PpuMI RGGWCCY 4 cut(s) 1267, 3744, 4347, 4398
PscI ACATGT 4 cut(s) 1015, 3201, 3491, 4103
PshBI ATTAAT 1 cut(s) 3030
Psp124BI GAGCTC 1 cut(s) 1447
Psp1406I AACGTT 1 cut(s) 967
Psp5II RGGWCCY 4 cut(s) 1267, 3744, 4347, 4398
Psp6I CCWGG 8 cut(s) 288, 713, 1114, 2911, 3039, 3973, 4210, 4245
PspFI CCCAGC 2 cut(s) 489, 1320
PspGI CCWGG 8 cut(s) 288, 713, 1114, 2911, 3039, 3973, 4210, 4245
PspLI CGTACG 1 cut(s) 2380
PspPI GGNCC 9 cut(s) 717, 1267, 2892, 3186, 3744, 3926, 4137, 4347, 4398
PspPPI RGGWCCY 4 cut(s) 1267, 3744, 4347, 4398
PsrI GAACNNNNNNTAC 2 cut(s) 2364, 2396
PstNI CAGNNNCTG 1 cut(s) 489
PvuII CAGCTG 1 cut(s) 2824
RruI TCGCGA 1 cut(s) 4224
RseI CAYNNNNRTG 3 cut(s) 1965, 2078, 3206
SacI GAGCTC 1 cut(s) 1447
SatI GCNGC 7 cut(s) 434, 474, 691, 2071, 2113, 2469, 4052
Sau96I GGNCC 9 cut(s) 717, 1267, 2892, 3186, 3744, 3926, 4137, 4347, 4398
SchI GAGTC 7 cut(s) 1397, 1816, 1927, 2068, 3200, 3903, 4375
SduI GDGCHC 5 cut(s) 408, 1447, 2161, 2321, 3736
SfcI CTRYAG 3 cut(s) 2386, 3784, 3798
SfoI GGCGCC 1 cut(s) 3449
SinI GGWCC 5 cut(s) 1267, 3744, 4137, 4347, 4398
SmiMI CAYNNNNRTG 3 cut(s) 1965, 2078, 3206
SmlI CTYRAG 6 cut(s) 1475, 1949, 2029, 2054, 2123, 3452
SmoI CTYRAG 6 cut(s) 1475, 1949, 2029, 2054, 2123, 3452
SseBI AGGCCT 1 cut(s) 1094
SspDI GGCGCC 1 cut(s) 3447
SspMI CTAG 8 cut(s) 890, 908, 1005, 1310, 1337, 1433, 2067, 3411
SstI GAGCTC 1 cut(s) 1447
StuI AGGCCT 1 cut(s) 1094
StyI CCWWGG 2 cut(s) 2204, 3221
TaiI ACGT 3 cut(s) 970, 2382, 2425
TaqII GACCGA 2 cut(s) 2780, 4154
TatI WGTACW 5 cut(s) 802, 925, 1147, 2510, 4106
TauI GCSGC 3 cut(s) 476, 693, 2471
TscAI CASTG 8 cut(s) 490, 1226, 2191, 2951, 3093, 3487, 4168, 4309
TseFI GTSAC 4 cut(s) 1202, 1400, 3482, 3494
TseI GCWGC 4 cut(s) 433, 2070, 2112, 4051
Tsp45I GTSAC 4 cut(s) 1202, 1400, 3482, 3494
TspGWI ACGGA 3 cut(s) 1607, 1653, 2000
TspRI CASTG 8 cut(s) 490, 1226, 2191, 2951, 3093, 3487, 4168, 4309
Van91I CCANNNNNTGG 1 cut(s) 4252
Vha464I CTTAAG 2 cut(s) 1949, 2054
VpaK11BI GGWCC 5 cut(s) 1267, 3744, 4137, 4347, 4398
VspI ATTAAT 1 cut(s) 3030
XagI CCTNNNNNAGG 1 cut(s) 2873
XapI RAATTY 8 cut(s) 253, 732, 743, 818, 1876, 2353, 2922, 3284
XbaI TCTAGA 1 cut(s) 1336
XceI RCATGY 6 cut(s) 1019, 1350, 2248, 3205, 3495, 4107
XmiI GTMKAC 1 cut(s) 147
XmnI GAANNNNTTC 2 cut(s) 1079, 1094
XspI CTAG 8 cut(s) 890, 908, 1005, 1310, 1337, 1433, 2067, 3411
Zsp2I ATGCAT 1 cut(s) 1485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.