Rroxscaffold_4G00305210

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000067
Physical Location & Seq
Forward (+)
25860203 .. 25867517
7315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00305210.1

Sequence Viewer

Length: 4890 bp
ATGGACCTTGCTTCCTCTTCTTCTTCTTCATTGACCTATGATGGCTTCCTCAGTTTTAGAGGCGAAGACACCCGACAGAGCTTTGTCTGCCATCTTCACAAAGCTCTGGAACTGAAGGCACTCAAAGTCTACATTGATAAAGAAGATCTGAAAAAAGGCGACCGCCTTTCGGATCTACTGGAAGCCATTGCCGAATCAAAGCTTTCGATTGTAGTTTTCTCTGAGAACTACGGGAATTCCACATGGTGCTTGAAAGAACTGGTGCAGATTATGAAATGCAAGGAGAAGCAGCACCAGATTGTGATGCCCGTTTTCTACAAAGTAGATCCTTCCCACATTCGTAAACAGACGGGAAGTTTTGGTGAAGCTTTTGCCAAGCATGAACGCGACCGCGAAAGGGACGAGGAGTTGCTGCAAGAAGTGCAGACATGGAGATCCGCTTTAAGGGAAGCCGCCGACTTAAGTGGCTGGGATTCCAAAAATTATGAGGATGATGCCAAGCTCATTGAGCGTATTGTAAATGATGTCCTTGATAAAGTGAATCGGATCTCGCCACACATAGAGGATGGCTTGGTTGCAATGGATTCCCATCTGCGTCAAGTGGGACGACTATTAAATGATGCGCCGGATGATGTTGTCTGTGTCGGAATCTGGGGAATGGGCGGCTTGGGTAAAACAACCATTGCCAGGGCTGTTTATGACAGAATTTGTCATAATTTTGACCACCGTTGCTTTCTTCAAAATGTGAGGGAAGGCTTCAAGAGGAAAGGTGAAGTGAAAATGCAACTTAAATTTCTATCTGGAATCTTGAACGAAAAAGTCAGCAATTTTGATGGAGGGTACATGAAGATGCTGAAAAGGCTAGGTGACAAAAAAATCCTGGTTGTTCTTGACGATGTACTCGATTCAAGCCAGATTGATACCTTACTCGGAGAGAAACGTTCATTTGGTGGTGGAAGTATAATTATTGTAACAACTAGAGATGAACATGTGGTGCGTGGATTTGAGATATACAAGCCCGAGCCATTAAGTGATGAAGATGCTCTGAACCTCTTCAGCCAGAAGGCCTTCGACACATGCAAACCCCCTGGAGAGTATGAACATCGCTCAAAGTGCATAGTAGAATATGCACGCGGTCTGCCCTTAGCACTTACAGTATTGGGAAAGTTTCTTTGTTGTAAGAGAAGTGTATCTCACTGGGAAGATCAGCTCAAGAAATTGAAGGAATGTGATCCGCACGAAGGTATTGAGAACGTGCTTAAAATAAGCTATGATGGACTAAAGCCTTGTCAGCAGGTCATATTTCTAGATATTGCATGTTTCTTTAAAGGAATGGAGAAAGACTATGTAACAAAAGTTCTTGCCAATTGTGACTGCATCAGTCCCGGCCTTGACTTAGATGTTCTAGTTGAAAGAGCTCTTGTAACTGTCTCATATTCCAATCAACTTGAGATGCACGATTTACTACAAGAAATGGGTAGACAAATTGAAAAAGACGGGAAGCGGAGTAGATTTTGGGGTGAAGAAGCTGAGCGTGTGCTTACTGAAAATACGGCTACGGAAAATGCTGGACGCTTGATGATTGACTTGCCAAAAAAGGAAGGCGGATCCTTCAATACTTCAATGCGCATATTCACTTACAGGCAAAGGTATGCTGGAGATGATGGTGAAAATCACCTGACTGGGAACTTCAAGTTTCTTTGTCGTGGGTTGAAGGTTCTCGTCTGGCATGGATATCCCCAAAAGTGTTTGCCATCCTACTTTGACCCCAAGAATCTTGTTCAGCTTGACTTGCGTTACAGTAACATTAAACATCGTCTTTCGAAAGGATCCAAGCCGGCGAAAAAGTTGGCAATCATCGATTTGAGTGGCTCTCCATGTGTTAAAAGAATCCCTGACTTGACTCTTGCGACAAATCTAAAGGAACTCCATTTTAACGGTTGTTCAAGTTTAAGGGAGGTTCGCCCATCCATTTCATCTCTTAAAAACCTTGTTTTATTTAGTTTAGCAGAGTGCCGTGAACTTAAGAGTCTTCCTGGCAGCATTGATCAAATGAAGTCTCTCAAAACCCTTGATCTTCACCACTGCTTAAATTTTGAGATATTTCCAGAGATTTCGGAAGTTATGGAGGCACTATCAAAGCTTGATTTATTCAAAACCGCAATTAAGGAGTTACCATCGTCAATTGAACGGCTTCGGGGGCTTAAATCATTAGACATGACATTCTGTAGAAGCCTTATCCGTCTTCCAGACAGTGTCTGTAATTTAGCAGAACTTAAATCTCTCAACCTGGGATGGTGCGAAAAACTTAGTAAGTTGCCTGAGAACATTGGGAATTTAGAATCTCTGTTGGAACTCCAAGTAAATTGCAGTGGTTTAGAACAACTACCTGTATCTATAATACGTTTGAAACAGGGTCGATTATCGTTTTCTAGTTGCGGACAAATGGCGGCTCCCCTTTCAGCATGGCCATCATCAATTGAAGATTGTTGTACTGCTGTGGTAAATCTTGATTTCAGTGGTTGCAATCTGAAGGAGTTATCGGATGCCATTGCTTATTTTCGTTCATTAAAAGTATTGAATTTGTCAGGAAATAGGAATCTGAGGAGCTTGCCTGCAGCCATGAATCAACTGCGTTGCTTAGAACAGCTTAAATTGGAAGATTGCCAGAGGCTGAGATCAATACCAGAGCTTTCATCAAGAATAAATTACATAAATGCGCATAATTGCACAGCTTTGGAAGCTGTTTCAACACCACAGTCTCCCTACGATATCGGTCGGTACTTCATATTTTCTAATTGCCATCTGCTGGTGCAGAAAGATCTTTTCAGAGATATTGTAGAAACTCATTCCCCTCCTCAGCGTAATTGTTCCCGACCCTTTCATGTCTATTTCCCTGGAAGTGAAATTCCAGAGTGGTTCACCCATCAGTGTAGGGGACCTTCCGTAACTGTCAAGCTACTACAACCAAATTGTTTTGACAACAAGTTTTTGGGCTTTACTATATGCGCGGTTACTAATAAGCCCGAGGAGATGATTGCGAGTAACGTTTCAGGTCCACATCGTTATTTTAGACTGACTGCTCGATGTTTCTGTACCTTCAAAGGAGATCTTTGCGAGTACCGTTTCAGTTTCTATATGTTTGATGTTGTTTCTCGCGGAAGTGAATTGTTTGAGTCAAATCACATGTTACTGGGATATGTGCCATGGTCTGAATCTGGCATGATTAAGACAGAGGAAGGCAATGAACGCCGTTACACAGAGGCCAAATTTGAGATACAATTGGACTATGAACCGTGTAGTAGTAGATCATTTCGATTGGGTAATGGATTTGACCCGTGCATCGAAAGGTGCGGAGTTCGATTCGTTTTTGCCAACAGTGAGGAAGATTTCGGGCAACCAATGGCAGAAGTTGATAATTCTGAGAGGAGCTTTGAGGGGTGCACGGCCGAACCCAGTGGCACTGACATCACAAATGAGGATGAGCAATACCTTAAATTATCACAAGTTTTTAAAGGCGCAAACAGACCACCGAGTTACATCAAAATTGAGGACAATGATTTATCTGGGAAGAGGAAAACTCTGGAAAGGTGGACGCAAAAAGTCAGAGTTACATCGGGTCTGATGGAAGGGAGAGTGGATGGTTTTAAATGGTCATGGGGTGGCGGTGCAATCGGCGGGAATACGTATTATTGTGGTGAACGCGTACAACGTCGCTGTTGGGCAAAAGCCATACAGCCACCTCACGATGACCCAACAATCTTTCAAATTACTTACGTTGGGAGGCACACTTGTTGGAAATTTCAACCAATGCTAACACACCAAGTAGGAGTTACACCGGGTATGAGGATTGAAGGTCCTTTTGATGGCTTTACATGGGGGAAGTATGACCAAACGGACATACCAGGAGCTGAATACCCGAGAGTCTATTATTACGTATGCACTCCTCAAAATGTCAAAGGCTGCATGGCCATAAAGGAAGTGCAACACTCCAGTGATCAAAAAATCCTGAAAATTACTTACAGAGGAAGGCACACATGTATAGAAGCCTCCGACAGCATTGCAGGATGGTTTGAAAGACTACACTCCAAGGGCAGTGTTGAATCAAGTTTTCATGAACAAGTTCTCCTATCACCAAGCTCACCAGGAGCCAACTCAAAAGATATGTCAAATAGAAAGGAAGTTACTGCAAAGAAAAGCAAAGGTGAAGAAGTGGCTGAGCTTGAAGTTGAGAAGACAGAAAAGAAGATGAAGAAGAAGAAAAAGGATAAAGACAATGGTGTTTTGGGATCTTCAGATGGGGAGAAATCTGTGAAGGTGAAGAGGCACAAAGGTAAAGAAGAAGCTGGTTCACCTCAAACTGAAAAGTCTGCAAAGATGAAAAAGAAGAAAAACAAAGAGGCTCAGGATCATGGTGCTGATGCTGCCACTGATAATGGCAAGGGTGATGGTGAAGCTGATAAGAGTGGGAAAAAGAAGAAGGAGAAGAAGAAGCGGAAACAAGAGGAAATAACTGACAGTCCTGTTTCAGTTCCTGCAAAGAAAAGCAAATGTGAAGAAGCGGCTGAGCTTAAAGTTGAGAAGACAGAAAAGAAGAAGAAGAAAAAGGATAAAGACAATGGTGTTTTGGATTGTTCAGATGGGGAGAAATCTGTGAATGTGAAGAAGCACAAAGATAAAGAAGAGGCTGGTTCACCTCAAACTGAAAAGTCTGAAAAGAAGAAAAACAAAGAGGCTGAGAATCATGGCGCTAAAGCTGCCACTGATAATGGCAAGGGTGTGATGGTGAGGCTGATAAGAGTGAAAAAGAAGGAGAAGAAGAAGCGCAAACAAGAGGAAATAACTGACAGTCCTGTTACAGTTCCTGCAAAGAAAAGCAAATTAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1629

Amino Acids

184.26

Weight (kDa)

8.63

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 13 - 188 3.3e-53 TIR domain
TIR_2 PF13676 16 - 110 1.7e-15 TIR domain
NB-ARC PF00931 199 - 358 2.8e-18 NB-ARC domain
WHD_ROQ1 PF23282 431 - 497 1.5e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 653 - 764 2.8e-07 Leucine-rich repeat region
LRR_13 PF23286 686 - 770 3.3e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 715 - 788 3.5e-10 Leucine-rich repeat region
LRR_14 PF23598 752 - 805 2.3e-07 Leucine-rich repeat region
C-JID PF20160 965 - 1127 1.2e-15 C-JID domain
WRKY PF03106 1289 - 1347 4.2e-15 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 1628, 2721
Acc36I ACCTGC 1 cut(s) 1285
AccB7I CCANNNNNTGG 1 cut(s) 2809
AccI GTMKAC 2 cut(s) 129, 1480
AccII CGCG 6 cut(s) 387, 393, 1134, 3011, 3159, 3705
AclI AACGTT 2 cut(s) 940, 3048
AcoI YGGCCR 3 cut(s) 2468, 3447, 3969
AcsI RAATTY 9 cut(s) 235, 705, 791, 2092, 2335, 2581, 2907, 3269, 3800
AcuI CTGAAG 4 cut(s) 134, 1039, 2552, 4278
AdeI CACNNNGTG 1 cut(s) 246
AfaI GTAC 7 cut(s) 842, 900, 2494, 2783, 3097, 3122, 3708
AfiI CCNNNNNNNGG 7 cut(s) 169, 397, 444, 687, 1241, 2809, 3866
AflII CTTAAG 2 cut(s) 460, 2024
AflIII ACRYGT 4 cut(s) 988, 3186, 3703, 4037
AjnI CCWGG 8 cut(s) 686, 879, 1087, 2035, 2289, 2896, 3904, 4144
AleI CACNNNNGTG 1 cut(s) 3993
AloI GAACNNNNNNTCC 2 cut(s) 4110, 4142
Alw21I GWGCWC 2 cut(s) 1420, 3446
Alw26I GTCTC 3 cut(s) 1435, 2064, 2766
Alw44I GTGCAC 1 cut(s) 3442
AlwNI CAGNNNCTG 5 cut(s) 2259, 2674, 3911, 4535, 4865
Ama87I CYCGRG 3 cut(s) 1019, 3026, 3919
AoxI GGCC 6 cut(s) 1065, 1387, 2468, 3264, 3447, 3969
ApaLI GTGCAC 1 cut(s) 3442
ApeKI GCWGC 7 cut(s) 289, 412, 2040, 2618, 3963, 4424, 4757
ApoI RAATTY 9 cut(s) 235, 705, 791, 2092, 2335, 2581, 2907, 3269, 3800
ArsI GACNNNNNNTTYG 2 cut(s) 4151, 4183
Asp700I GAANNNNTTC 4 cut(s) 1052, 1067, 2193, 4122
AspLEI GCGC 7 cut(s) 625, 1629, 2722, 3011, 3521, 4751, 4827
AspS9I GGNCC 4 cut(s) 4, 2939, 3056, 3857
AsuC2I CCSGG 2 cut(s) 1386, 3840
AsuII TTCGAA 1 cut(s) 1823
AvaI CYCGRG 3 cut(s) 1019, 3026, 3919
AvaII GGWCC 4 cut(s) 4, 2939, 3056, 3857
BaeGI GKGCMC 1 cut(s) 3446
BalI TGGCCA 2 cut(s) 2470, 3971
BamHI GGATCC 2 cut(s) 1607, 1829
BanII GRGCYC 1 cut(s) 1420
BarI GAAGNNNNNNTAC 2 cut(s) 2926, 2958
BbsI GAAGAC 5 cut(s) 72, 2024, 2237, 4241, 4589
Bbv12I GWGCWC 2 cut(s) 1420, 3446
BbvCI CCTCAGC 1 cut(s) 2859
BbvI GCAGC 7 cut(s) 301, 399, 2052, 2630, 3950, 4411, 4744
BceAI ACGGC 5 cut(s) 1569, 2001, 2207, 3237, 3462
BcgI CGANNNNNNTGC 2 cut(s) 4043, 4077
BciT130I CCWGG 8 cut(s) 688, 881, 1089, 2037, 2291, 2898, 3906, 4146
BclI TGATCA 2 cut(s) 2047, 3997
BcnI CCSGG 2 cut(s) 1386, 3840
BcoDI GTCTC 3 cut(s) 1435, 2064, 2766
BfaI CTAG 5 cut(s) 863, 978, 1307, 1406, 2433
BfmI CTRYAG 2 cut(s) 2227, 2616
BfoI RGCGCY 1 cut(s) 4752
BfrI CTTAAG 2 cut(s) 460, 2024
BfuAI ACCTGC 1 cut(s) 1285
BglII AGATCT 3 cut(s) 145, 2821, 3109
BlpI GCTNAGC 3 cut(s) 1530, 4218, 4566
Bme18I GGWCC 4 cut(s) 4, 2939, 3056, 3857
BmeT110I CYCGRG 3 cut(s) 1019, 3026, 3919
BmgT120I GGNCC 4 cut(s) 4, 2939, 3056, 3857
BmiI GGNNCC 5 cut(s) 1609, 1831, 2454, 2940, 4150
BmrI ACTGGG 4 cut(s) 1207, 1692, 3203, 3450
BmuI ACTGGG 4 cut(s) 1207, 1692, 3203, 3450
BpiI GAAGAC 5 cut(s) 72, 2024, 2237, 4241, 4589
BplI GAGNNNNNCTC 4 cut(s) 1858, 1890, 3565, 3597
BpmI CTGGAG 3 cut(s) 1110, 1677, 3976
Bpu10I CCTNAGC 3 cut(s) 1144, 2859, 4404
Bpu1102I GCTNAGC 3 cut(s) 1530, 4218, 4566
Bpu14I TTCGAA 1 cut(s) 1823
BpuEI CTTGAG 2 cut(s) 1196, 1469
BpuMI CCSGG 2 cut(s) 1386, 3840
Bsa29I ATCGAT 1 cut(s) 1860
BsaAI YACGTR 2 cut(s) 3687, 3937
BsaBI GATNNNNATC 1 cut(s) 588
BsaJI CCNNGG 7 cut(s) 687, 1087, 2290, 2896, 3027, 3206, 4089
BsaXI ACNNNNNCTCC 2 cut(s) 4110, 4140
Bsc4I CCNNNNNNNGG 7 cut(s) 169, 397, 444, 687, 1241, 2809, 3866
Bse118I RCCGGY 1 cut(s) 1837
Bse1I ACTGG 7 cut(s) 183, 264, 1202, 1687, 3198, 3456, 3993
Bse3DI GCAATG 6 cut(s) 186, 585, 681, 2550, 3250, 4059
Bse8I GATNNNNATC 1 cut(s) 588
BseBI CCWGG 8 cut(s) 688, 881, 1089, 2037, 2291, 2898, 3906, 4146
BseCI ATCGAT 1 cut(s) 1860
BseDI CCNNGG 7 cut(s) 687, 1087, 2290, 2896, 3027, 3206, 4089
BseJI GATNNNNATC 1 cut(s) 588
BseLI CCNNNNNNNGG 7 cut(s) 169, 397, 444, 687, 1241, 2809, 3866
BseMI GCAATG 6 cut(s) 186, 585, 681, 2550, 3250, 4059
BseNI ACTGG 7 cut(s) 183, 264, 1202, 1687, 3198, 3456, 3993
BseRI GAGGAG 6 cut(s) 419, 2620, 2847, 3044, 3442, 3936
BseSI GKGCMC 1 cut(s) 3446
BseX3I CGGCCG 1 cut(s) 3447
BseXI GCAGC 7 cut(s) 301, 399, 2052, 2630, 3950, 4411, 4744
BseYI CCCAGC 1 cut(s) 468
BsgI GTGCAG 3 cut(s) 284, 443, 2834
Bsh1236I CGCG 6 cut(s) 387, 393, 1134, 3011, 3159, 3705
Bsh1285I CGRYCG 4 cut(s) 163, 391, 2779, 3450
BshFI GGCC 6 cut(s) 1067, 1389, 2470, 3266, 3449, 3971
BshVI ATCGAT 1 cut(s) 1860
BsiEI CGRYCG 4 cut(s) 163, 391, 2779, 3450
BsiHKAI GWGCWC 2 cut(s) 1420, 3446
BsiHKCI CYCGRG 3 cut(s) 1019, 3026, 3919
BsiSI CCGG 4 cut(s) 626, 1386, 1838, 3839
BslFI GGGAC 4 cut(s) 413, 618, 1368, 2952
BslI CCNNNNNNNGG 7 cut(s) 169, 397, 444, 687, 1241, 2809, 3866
BsmAI GTCTC 3 cut(s) 1435, 2064, 2766
BsmFI GGGAC 4 cut(s) 413, 618, 1368, 2952
BsnI GGCC 6 cut(s) 1067, 1389, 2470, 3266, 3449, 3971
BsoBI CYCGRG 3 cut(s) 1019, 3026, 3919
Bsp119I TTCGAA 1 cut(s) 1823
Bsp1286I GDGCHC 2 cut(s) 1420, 3446
Bsp1720I GCTNAGC 3 cut(s) 1530, 4218, 4566
Bsp19I CCATGG 1 cut(s) 3206
BspANI GGCC 6 cut(s) 1067, 1389, 2470, 3266, 3449, 3971
BspDI ATCGAT 1 cut(s) 1860
BspFNI CGCG 6 cut(s) 387, 393, 1134, 3011, 3159, 3705
BspHI TCATGA 1 cut(s) 4114
BspLI GGNNCC 5 cut(s) 1609, 1831, 2454, 2940, 4150
BspMAI CTGCAG 1 cut(s) 2620
BspMI ACCTGC 1 cut(s) 1285
BspT104I TTCGAA 1 cut(s) 1823
BspTI CTTAAG 2 cut(s) 460, 2024
BsrDI GCAATG 6 cut(s) 186, 585, 681, 2550, 3250, 4059
BsrFI RCCGGY 1 cut(s) 1837
BsrI ACTGG 7 cut(s) 183, 264, 1202, 1687, 3198, 3456, 3993
BssAI RCCGGY 1 cut(s) 1837
BssECI CCNNGG 7 cut(s) 687, 1087, 2290, 2896, 3027, 3206, 4089
BssT1I CCWWGG 2 cut(s) 3206, 4089
Bst2UI CCWGG 8 cut(s) 688, 881, 1089, 2037, 2291, 2898, 3906, 4146
Bst6I CTCTTC 5 cut(s) 22, 1058, 3566, 4316, 4677
BstAFI CTTAAG 2 cut(s) 460, 2024
BstAPI GCANNNNNTGC 1 cut(s) 421
BstBAI YACGTR 2 cut(s) 3687, 3937
BstBI TTCGAA 1 cut(s) 1823
BstC8I GCNNGC 4 cut(s) 1132, 1839, 2612, 2616
BstDSI CCRYGG 1 cut(s) 3206
BstFNI CGCG 6 cut(s) 387, 393, 1134, 3011, 3159, 3705
BstH2I RGCGCY 1 cut(s) 4752
BstHHI GCGC 7 cut(s) 625, 1629, 2722, 3011, 3521, 4751, 4827
BstMAI GTCTC 3 cut(s) 1435, 2064, 2766
BstMCI CGRYCG 4 cut(s) 163, 391, 2779, 3450
BstNI CCWGG 8 cut(s) 688, 881, 1089, 2037, 2291, 2898, 3906, 4146
BstNSI RCATGY 5 cut(s) 992, 1080, 1320, 3190, 4041
BstSFI CTRYAG 2 cut(s) 2227, 2616
BstSLI GKGCMC 1 cut(s) 3446
BstSNI TACGTA 2 cut(s) 3687, 3937
BstUI CGCG 6 cut(s) 387, 393, 1134, 3011, 3159, 3705
BstV1I GCAGC 7 cut(s) 301, 399, 2052, 2630, 3950, 4411, 4744
BstV2I GAAGAC 5 cut(s) 72, 2024, 2237, 4241, 4589
BstZI CGGCCG 1 cut(s) 3447
Bsu15I ATCGAT 1 cut(s) 1860
BsuRI GGCC 6 cut(s) 1067, 1389, 2470, 3266, 3449, 3971
BsuTUI ATCGAT 1 cut(s) 1860
BtgI CCRYGG 1 cut(s) 3206
BtgZI GCGATG 1 cut(s) 1088
BtsI GCAGTG 3 cut(s) 2083, 2377, 4102
BveI ACCTGC 1 cut(s) 1285
Cac8I GCNNGC 4 cut(s) 1132, 1839, 2612, 2616
CaiI CAGNNNCTG 5 cut(s) 2259, 2674, 3911, 4535, 4865
CciI TCATGA 1 cut(s) 4114
CfoI GCGC 7 cut(s) 625, 1629, 2722, 3011, 3521, 4751, 4827
Cfr10I RCCGGY 1 cut(s) 1837
Cfr13I GGNCC 4 cut(s) 4, 2939, 3056, 3857
ClaI ATCGAT 1 cut(s) 1860
CseI GACGC 3 cut(s) 584, 1581, 3604
Csp6I GTAC 7 cut(s) 841, 899, 2493, 2782, 3096, 3121, 3707
CviQI GTAC 7 cut(s) 841, 899, 2493, 2782, 3096, 3121, 3707
DraI TTTAAA 3 cut(s) 1327, 3514, 3649
DraIII CACNNNGTG 1 cut(s) 246
EaeI YGGCCR 3 cut(s) 2468, 3447, 3969
EagI CGGCCG 1 cut(s) 3447
Eam1104I CTCTTC 5 cut(s) 22, 1058, 3566, 4316, 4677
EarI CTCTTC 5 cut(s) 22, 1058, 3566, 4316, 4677
EciI GGCGGA 1 cut(s) 1620
Ecl136II GAGCTC 1 cut(s) 1418
EclXI CGGCCG 1 cut(s) 3447
Eco105I TACGTA 2 cut(s) 3687, 3937
Eco130I CCWWGG 2 cut(s) 3206, 4089
Eco147I AGGCCT 1 cut(s) 1067
Eco24I GRGCYC 1 cut(s) 1420
Eco32I GATATC 2 cut(s) 1736, 2773
Eco47I GGWCC 4 cut(s) 4, 2939, 3056, 3857
Eco52I CGGCCG 1 cut(s) 3447
Eco53kI GAGCTC 1 cut(s) 1418
Eco57I CTGAAG 4 cut(s) 134, 1039, 2552, 4278
Eco88I CYCGRG 3 cut(s) 1019, 3026, 3919
EcoICRI GAGCTC 1 cut(s) 1418
EcoO109I RGGNCCY 2 cut(s) 2939, 3857
EcoRI GAATTC 1 cut(s) 235
EcoRII CCWGG 8 cut(s) 686, 879, 1087, 2035, 2289, 2896, 3904, 4144
EcoRV GATATC 2 cut(s) 1736, 2773
EcoT14I CCWWGG 2 cut(s) 3206, 4089
EcoT38I GRGCYC 1 cut(s) 1420
ErhI CCWWGG 2 cut(s) 3206, 4089
FalI AAGNNNNNCTT 2 cut(s) 3096, 3128
FaqI GGGAC 4 cut(s) 413, 618, 1368, 2952
FauI CCCGC 1 cut(s) 3671
FbaI TGATCA 2 cut(s) 2047, 3997
FblI GTMKAC 2 cut(s) 129, 1480
FriOI GRGCYC 1 cut(s) 1420
FspAI RTGCGCAY 2 cut(s) 1628, 2721
FspBI CTAG 5 cut(s) 863, 978, 1307, 1406, 2433
FspI TGCGCA 2 cut(s) 1628, 2721
GlaI GCGC 7 cut(s) 624, 1628, 2721, 3010, 3520, 4750, 4826
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 3 cut(s) 1110, 1677, 3976
HaeII RGCGCY 1 cut(s) 4752
HaeIII GGCC 6 cut(s) 1067, 1389, 2470, 3266, 3449, 3971
HapII CCGG 4 cut(s) 626, 1386, 1838, 3839
HgaI GACGC 3 cut(s) 584, 1581, 3604
HhaI GCGC 7 cut(s) 625, 1629, 2722, 3011, 3521, 4751, 4827
Hin6I GCGC 7 cut(s) 623, 1627, 2720, 3009, 3519, 4749, 4825
HinP1I GCGC 7 cut(s) 623, 1627, 2720, 3009, 3519, 4749, 4825
HindIII AAGCTT 3 cut(s) 200, 366, 2141
HpaII CCGG 4 cut(s) 626, 1386, 1838, 3839
Hpy99I CGWCG 1 cut(s) 3717
HpyCH4IV ACGT 8 cut(s) 940, 1254, 2404, 3048, 3686, 3712, 3777, 3936
HpySE526I ACGT 8 cut(s) 940, 1254, 2404, 3048, 3686, 3712, 3777, 3936
HspAI GCGC 7 cut(s) 623, 1627, 2720, 3009, 3519, 4749, 4825
KroI GCCGGC 1 cut(s) 1837
KroNI GCCGGC 1 cut(s) 1839
Ksp22I TGATCA 2 cut(s) 2047, 3997
LmnI GCTCC 5 cut(s) 2458, 2607, 3429, 3908, 4148
Lsp1109I GCAGC 7 cut(s) 301, 399, 2052, 2630, 3950, 4411, 4744
MaeI CTAG 5 cut(s) 863, 978, 1307, 1406, 2433
MaeII ACGT 8 cut(s) 940, 1254, 2404, 3048, 3686, 3712, 3777, 3936
MfeI CAATTG 4 cut(s) 1366, 2184, 2478, 3281
MhlI GDGCHC 2 cut(s) 1420, 3446
MlsI TGGCCA 2 cut(s) 2470, 3971
MluI ACGCGT 1 cut(s) 3703
MluNI TGGCCA 2 cut(s) 2470, 3971
MlyI GAGTC 4 cut(s) 1897, 2038, 3185, 3933
MmeI TCCRAC 4 cut(s) 625, 2331, 3776, 4077
Mox20I TGGCCA 2 cut(s) 2470, 3971
MroNI GCCGGC 1 cut(s) 1837
MroXI GAANNNNTTC 4 cut(s) 1052, 1067, 2193, 4122
MscI TGGCCA 2 cut(s) 2470, 3971
MslI CAYNNNNRTG 2 cut(s) 848, 3993
Msp20I TGGCCA 2 cut(s) 2470, 3971
MspCI CTTAAG 2 cut(s) 460, 2024
MspI CCGG 4 cut(s) 626, 1386, 1838, 3839
MunI CAATTG 4 cut(s) 1366, 2184, 2478, 3281
MvaI CCWGG 8 cut(s) 688, 881, 1089, 2037, 2291, 2898, 3906, 4146
MvnI CGCG 6 cut(s) 387, 393, 1134, 3011, 3159, 3705
NaeI GCCGGC 1 cut(s) 1839
NciI CCSGG 2 cut(s) 1386, 3840
NcoI CCATGG 1 cut(s) 3206
NgoMIV GCCGGC 1 cut(s) 1837
NlaIV GGNNCC 5 cut(s) 1609, 1831, 2454, 2940, 4150
NmuCI GTSAC 2 cut(s) 866, 1370
NsbI TGCGCA 2 cut(s) 1628, 2721
NspI RCATGY 5 cut(s) 992, 1080, 1320, 3190, 4041
NspV TTCGAA 1 cut(s) 1823
OliI CACNNNNGTG 1 cut(s) 3993
PagI TCATGA 1 cut(s) 4114
PceI AGGCCT 1 cut(s) 1067
PciI ACATGT 3 cut(s) 988, 3186, 4037
PcsI WCGNNNNNNNCGW 2 cut(s) 900, 3709
PdiI GCCGGC 1 cut(s) 1839
PdmI GAANNNNTTC 4 cut(s) 1052, 1067, 2193, 4122
PflFI GACNNNGTC 1 cut(s) 2255
PflMI CCANNNNNTGG 1 cut(s) 2809
PleI GAGTC 4 cut(s) 1897, 2037, 3184, 3932
PpsI GAGTC 4 cut(s) 1897, 2037, 3184, 3932
Ppu21I YACGTR 2 cut(s) 3687, 3937
PpuMI RGGWCCY 2 cut(s) 2939, 3857
PscI ACATGT 3 cut(s) 988, 3186, 4037
Psp124BI GAGCTC 1 cut(s) 1420
Psp1406I AACGTT 2 cut(s) 940, 3048
Psp5II RGGWCCY 2 cut(s) 2939, 3857
Psp6I CCWGG 8 cut(s) 686, 879, 1087, 2035, 2289, 2896, 3904, 4144
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 8 cut(s) 686, 879, 1087, 2035, 2289, 2896, 3904, 4144
PspN4I GGNNCC 5 cut(s) 1609, 1831, 2454, 2940, 4150
PspPI GGNCC 4 cut(s) 4, 2939, 3056, 3857
PspPPI RGGWCCY 2 cut(s) 2939, 3857
PsrI GAACNNNNNNTAC 2 cut(s) 1341, 1373
PstI CTGCAG 1 cut(s) 2620
PstNI CAGNNNCTG 5 cut(s) 2259, 2674, 3911, 4535, 4865
PsyI GACNNNGTC 1 cut(s) 2255
RsaI GTAC 7 cut(s) 842, 900, 2494, 2783, 3097, 3122, 3708
RsaNI GTAC 7 cut(s) 841, 899, 2493, 2782, 3096, 3121, 3707
RseI CAYNNNNRTG 2 cut(s) 848, 3993
SacI GAGCTC 1 cut(s) 1420
Sau96I GGNCC 4 cut(s) 4, 2939, 3056, 3857
SchI GAGTC 4 cut(s) 1897, 2038, 3185, 3933
SduI GDGCHC 2 cut(s) 1420, 3446
SfcI CTRYAG 2 cut(s) 2227, 2616
SfuI TTCGAA 1 cut(s) 1823
SinI GGWCC 4 cut(s) 4, 2939, 3056, 3857
SmiMI CAYNNNNRTG 2 cut(s) 848, 3993
SmlI CTYRAG 4 cut(s) 460, 1211, 1448, 2024
SmoI CTYRAG 4 cut(s) 460, 1211, 1448, 2024
SnaBI TACGTA 2 cut(s) 3687, 3937
SseBI AGGCCT 1 cut(s) 1067
SspMI CTAG 5 cut(s) 863, 978, 1307, 1406, 2433
SstI GAGCTC 1 cut(s) 1420
StuI AGGCCT 1 cut(s) 1067
StyI CCWWGG 2 cut(s) 3206, 4089
TaiI ACGT 8 cut(s) 943, 1257, 2407, 3051, 3689, 3715, 3780, 3939
TaqII GACCGA 1 cut(s) 2765
TatI WGTACW 2 cut(s) 898, 2492
TauI GCSGC 4 cut(s) 455, 666, 2453, 4565
TseFI GTSAC 2 cut(s) 866, 1370
TseI GCWGC 7 cut(s) 289, 412, 2040, 2618, 3963, 4424, 4757
Tsp45I GTSAC 2 cut(s) 866, 1370
TspGWI ACGGA 4 cut(s) 1574, 2231, 2935, 3911
Tth111I GACNNNGTC 1 cut(s) 2255
Van91I CCANNNNNTGG 1 cut(s) 2809
Vha464I CTTAAG 2 cut(s) 460, 2024
VneI GTGCAC 1 cut(s) 3442
VpaK11BI GGWCC 4 cut(s) 4, 2939, 3056, 3857
XapI RAATTY 9 cut(s) 235, 705, 791, 2092, 2335, 2581, 2907, 3269, 3800
XbaI TCTAGA 1 cut(s) 1306
XceI RCATGY 5 cut(s) 992, 1080, 1320, 3190, 4041
XmiI GTMKAC 2 cut(s) 129, 1480
XmnI GAANNNNTTC 4 cut(s) 1052, 1067, 2193, 4122
XspI CTAG 5 cut(s) 863, 978, 1307, 1406, 2433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.