MD00G1020700.v1.1

regulation of response to stimulus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000067
Physical Location & Seq
Reverse (-)
3731631 .. 3736778
5148 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1020700.v1.1.491

Sequence Viewer

Length: 3642 bp
ATGGCTGCTGCTGCATCTTCTTCTTCCTCTGGCTCTCCTTGGAAATACGACGTGTTCATCAATTTCAGAGGGGAAGACACTCGCAGGTGCTTCGTCAGCCACCTCTACAAAGCCCTGAATCAGAAAGCAATCAACACCTTCATTGATGCCGAAGGGCTCCGAAAGGGCAACGACCTTTTGCAGCTACTGACGGCGATCCAAGATTCGCGCGTTTCAATCGTAGTTTTTTCTCAAAACTATGCTTCTTCCACATGGTGCTTGAAGGAGCTCGTCCAAATACTGGATTGTATGGATCGAAAGAACCAGATGGTTGTGCCCGTTTTTTACCAAGTGGATCCTTCTGATGTCCGCAAAGCAAAGAGGAGCTTTGAGGAAGCTTTTGCCGAGCATGAAGGCCATTCAAACACCGACATGGAAGAGGTGCGGAGGTGGAGGTCCGCTCTTAGTCGAGCCATCAATTTATCCGGCTGGGATTCGAAAAATTATGAGGATGATGTGAAGCTCATTGAGGAAATTGTAAAAGATATTTTTCAGAAATTGATCCGCACCTCGTCAAGTAAAGACGATGACTTGGTTGAAATGGATTCCCACATGCATGAAATGGACTTGCTATTACATCCTGCTCCTCAAATGGACGACGTTCGTGTTGTTGGAATATGGGGTATGGGTGGTGTAGGCAAAACCACCATAGCTAGAGCTGTTTATGAGGAAATTGCTTGTCAATTTGAAGCTTGTTGCTTTCTTGACAATGTTAAGGAAGAATTCTCCACTTGTGGTGCCGTACATATGCAGGAAAAATTTCTCTCTAGAATCCTGAATGAAAAGGTGCAGAGTTTAGGCACTTTGGATCGAGGTTACAGAATGATTTTGAAAAGGCTACAGCTGAAAAAGGTTTTGATTGTTCTTGATGATGTTGACGACTTATTTCAAATTGAAACCTTACTTGGAAAGCAACATTCATTTGGTGGTGGGAGTAGAATCATTATCACCACTAGAGATAAGCTAGTACTAAGTGGAGCTGATGCGATCTACAGCCCCAAGGTTCTAAGTGGTGATGGAGCTTTGGAACTCTTTAGCCAGTATGCCTTCCGAACAAAGCAACCCAAAAGAGACTATGACCATCTCTCAAGCCGTGCTGTACGATATGCTCAAGGTCTCCCTTTAGCACTTAAAGTCTTGGGGGCTTTTCTTTATAACAAATCTGTACAGGAGTGGGAAGAGGTGCTAGAGAAATTAAAGAAAATCCCGCAGAGGGGAATTCATGATGTGCTAAGAACAAGCTTCGATGGACTAGATGATTCTGAGAAGGACATATTTCTGGATATTGCATGTTTCTTTAAAGGAGCGGAAAAAGACAACGCAACAAAGGTTCTGGACGGTTGTGGCTTTTATCCCCATACTGGGCTAAGAGTTCTAATTGATCGAGCTCTTATAACTGTCTCATGGGAAAAATTGGAGATGCATGATTTACTAGAGGAAATGGGTGCTCATATAACTGTCTCATGGGAAAAATTGGAGATGCATGATTTACTAGAGGAAATGGGTCGGGAAATCGTTCGCCAAGAATCCATTAAAGAGCCTGGGAAACGCAGTAGGTTGTGGAATTATGAAGATGTTCATCATGTGCTAACTCAAAATAAGGCTACGGAAGCTGTTGAAAGTATAATCCTTGACCCTTCATTCTCAAAACCAGAAGTGGTATACTTAAGTTCCGAAGCTTTTGTTAAAATGACGAAATTAAGATTGCTCAAAATCCATTGCGACAGTGGATATTCATGTGAGAATTTAAAGTTTCTTCCGCATGAGTTAAGAAGTCTCATATGGAAGCATTTTCCCCTAAAGTCTTTACCGTCCAATTTTATTGCGAAGAATCTTGTTGAGCTTGACATGCAAAATAGTCTCATTGAACATCTTTGGGAAGGAGCCAAGCCTCTGGAAAATTTGAAAATTATCAACTTAACAAGTTCTCCTCACCTGAAGAAAACTCCAGACTTCACGGAGGCGAAAAATCTGGAGAAAGTAGTTTTTCGAAGTTGTACAAGTTTACTTGAGGTTCACCCATCCATTTCATCTCTCGAAAACCTGGTTCTTTTGGATTTCGAAAATTGTGGAAATCTTAAGATTTTTCCAAGCAGGATTGGTATGAAATCTCTTAGAACCCTTACACTTTCAGGGTGCGAAAACCTCGAGAAGTTTCCCGAAGTTTCAGATGTTATGCAGGATCTATCAGTGCTTTATTTAAATTCTGCTGGAATTAAAGAACTGCCCTCGTCAATTAGTAATCTTACGGGGCTTCTTGTCTTGAATATAAATGGTTGCAGAAATCTTGAGGGTCTTCCAAGCAGCATTTGTCACATGAAATCTCTTCAATACCTTGATGTTTCTAAGTTCTCAAATCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGAATATTCTATCTGAGCTTGATTTGAGTGGCACTGCAATTAAGGAATTGCCTGCGTTAGTTTTAAATCTCACCAATCTTGTTACTCTGAGGCTGAATGATTGTAGAGAACTTGAAAGTCTTCCAAGCAGCATTTGTCAAATGAAATCTCTTCAATACCTTGATGTTTCTGGGTGCTCAAACCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGAATATGCTATCTGAGCTTAATTTGAGTGGCACTGCAATTAAGGAACTACCTGTGTCAGTTTTAAATCTCACCAATCTTGTTACATTGACGCTGGATGATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTGTCGAATGAAATCTCTTCAATACCTTCATGTTTCTGGATGCTCAAATCTTGAGAAGTTTCCAGAAATTTCAGAGGTTATGACTAAGCTGTCTTGGCTTTATTTGGATGGCACTGCAATTAAGGAATTGCCTGCGTCAGTTTTAAATCTCACCAATCTTCTTACGCTATTGCTGAATGATTGCAGAGAACTTGAGAGTCTTCTAAGCAACATTTGTCACATGAAATCTCTTCAATACCTTGATGTTTCTGGGTGCTCAAACCTTAAGAAGTTTCCAGAAATTTCAGAGGTTATGAATATGCTATCTGAGCTTTATTTGGGTGGCACTGCAATTAAGGAACTGCATGCGTCAGTTTTAAATCTCACCAATCTTGTTACACTGAGGCTGAATGATTGCAAAGAACTTGAGAGTGTTCCAAGCAGCATTTGTCACATGAAATCTCTTCAATACCTTGATGTTTTTGGGTGCTCAAACCTTAAGAAGTTCCCAGAAATTTCAGAGGTTATGAATATGCTATCTAAGCTCTATTTGGGTGGCACTGCAATTAAGGAACTGCCTGCGTCAATTTTAAATCTCACCAATCTTGTTACTCTGAGGCTGAATGATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTGTCAAATGAAATCTCTTCAATACCTTGATGTTTCTGGGTGCTCAAACCTTAAAAAGTTTCCAGAAATATCAGAGGTTATGAATATGCTATCTGAGCTTTATATGGGTGGCACTGCAATTAAGGAACTGCCTGCATCAATTTTAAATCTCACCAATCTTGTTACTCTGAGGCTGAATGATTGCAGAGAACTTGAGAGTCTTCCAAGCAGCATTTCATTTGTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_3 LRR_5 LRR_8 NB-ARC TIR

Protein Analysis

1214

Amino Acids

136.69

Weight (kDa)

5.46

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 15 - 192 2.1e-54 TIR domain
TIR_2 PF13676 18 - 113 7.6e-15 TIR domain
NB-ARC PF00931 210 - 366 1.1e-18 NB-ARC domain
WHD_ROQ1 PF23282 434 - 499 1.7e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 676 - 807 2e-11 Leucine-rich repeat region
LRR_13 PF23286 716 - 784 1.6e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 786 - 871 1.7e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_Zer-1 PF25013 810 - 884 2e-06 Zer-1-like, Leucine-rich repeats
LRR_14 PF23598 811 - 885 4.3e-08 Leucine-rich repeat region
LRR_13 PF23286 858 - 942 8.1e-13 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 887 - 955 7e-09 Leucine-rich repeat region
LRR_13 PF23286 929 - 1014 2.1e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 982 - 1100 1.3e-09 Leucine-rich repeat region
LRR_13 PF23286 999 - 1085 1.3e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 1070 - 1156 1.3e-14 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 1100 - 1171 6.9e-09 Leucine-rich repeat region
LRR_13 PF23286 1142 - 1210 2.9e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1194, 1433
AarI CACCTGC 1 cut(s) 75
AasI GACNNNNNNGTC 1 cut(s) 2863
Acc36I ACCTGC 1 cut(s) 75
AccB1I GGYRCC 1 cut(s) 776
AccB7I CCANNNNNTGG 1 cut(s) 280
AccBSI CCGCTC 2 cut(s) 440, 1346
AccI GTMKAC 1 cut(s) 1701
AccII CGCG 2 cut(s) 208, 210
AciI CCGC 7 cut(s) 349, 424, 438, 544, 1247, 1346, 1799
AclWI GGATC 7 cut(s) 190, 300, 329, 342, 535, 855, 2229
AcuI CTGAAG 1 cut(s) 1997
AdeI CACNNNGTG 1 cut(s) 255
AfaI GTAC 5 cut(s) 783, 1008, 1140, 1206, 2038
AfiI CCNNNNNNNGG 5 cut(s) 280, 1252, 1253, 1400, 1401
AflII CTTAAG 4 cut(s) 1705, 2117, 3038, 3251
AflIII ACRYGT 1 cut(s) 51
AjiI CACGTC 1 cut(s) 52
AjnI CCWGG 2 cut(s) 1579, 2082
AjuI GAANNNNNNNTTGG 4 cut(s) 927, 959, 3610, 3642
Alw21I GWGCWC 7 cut(s) 270, 1429, 1489, 2606, 3032, 3245, 3458
Alw26I GTCTC 6 cut(s) 1104, 1160, 1444, 1504, 1820, 1904
AlwI GGATC 7 cut(s) 190, 300, 329, 342, 535, 855, 2229
AlwNI CAGNNNCTG 1 cut(s) 187
Ama87I CYCGRG 1 cut(s) 2186
AoxI GGCC 1 cut(s) 394
Asp700I GAANNNNTTC 4 cut(s) 798, 1554, 1614, 2547
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 1 cut(s) 435
AsuII TTCGAA 3 cut(s) 476, 2029, 2100
AvaI CYCGRG 1 cut(s) 2186
AvaII GGWCC 1 cut(s) 435
BaeGI GKGCMC 1 cut(s) 318
BamHI GGATCC 1 cut(s) 334
BanI GGYRCC 1 cut(s) 776
BanII GRGCYC 3 cut(s) 159, 270, 1429
BbsI GAAGAC 7 cut(s) 81, 2327, 2540, 2753, 2966, 3392, 3605
Bbv12I GWGCWC 7 cut(s) 270, 1429, 1489, 2606, 3032, 3245, 3458
BbvI GCAGC 7 cut(s) 193, 2355, 2568, 2781, 3207, 3420, 3633
BccI CCATC 7 cut(s) 301, 461, 1049, 1128, 1280, 2068, 2876
BceAI ACGGC 3 cut(s) 207, 764, 1116
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciT130I CCWGG 2 cut(s) 1581, 2084
BcoDI GTCTC 6 cut(s) 1104, 1160, 1444, 1504, 1820, 1904
BfaI CTAG 8 cut(s) 693, 807, 993, 1004, 1226, 1292, 1472, 1532
BfmI CTRYAG 2 cut(s) 878, 1030
BfrI CTTAAG 4 cut(s) 1705, 2117, 3038, 3251
BfuAI ACCTGC 1 cut(s) 75
BmcAI AGTACT 1 cut(s) 1008
Bme1390I CCNGG 2 cut(s) 1581, 2084
Bme18I GGWCC 1 cut(s) 435
BmeT110I CYCGRG 1 cut(s) 2186
BmgBI CACGTC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 4 cut(s) 158, 336, 778, 1924
BmrFI CCNGG 2 cut(s) 1581, 2084
BmrI ACTGGG 1 cut(s) 1410
BmsI GCATC 7 cut(s) 23, 136, 1012, 1449, 1509, 2804, 3557
BmuI ACTGGG 1 cut(s) 1410
BpiI GAAGAC 7 cut(s) 81, 2327, 2540, 2753, 2966, 3392, 3605
BplI GAGNNNNNCTC 2 cut(s) 424, 456
BpmI CTGGAG 2 cut(s) 1971, 2033
Bpu14I TTCGAA 3 cut(s) 476, 2029, 2100
BsaBI GATNNNNATC 1 cut(s) 1617
BsaI GGTCTC 1 cut(s) 1160
BsaJI CCNNGG 3 cut(s) 38, 1038, 1580
BsaXI ACNNNNNCTCC 2 cut(s) 1951, 1981
Bsc4I CCNNNNNNNGG 5 cut(s) 280, 1252, 1253, 1400, 1401
Bse1I ACTGG 3 cut(s) 285, 1078, 1405
Bse3DI GCAATG 1 cut(s) 1756
Bse8I GATNNNNATC 1 cut(s) 1617
BseBI CCWGG 2 cut(s) 1581, 2084
BseDI CCNNGG 3 cut(s) 38, 1038, 1580
BseGI GGATG 6 cut(s) 496, 616, 2060, 2743, 2819, 2887
BseJI GATNNNNATC 1 cut(s) 1617
BseLI CCNNNNNNNGG 5 cut(s) 280, 1252, 1253, 1400, 1401
BseMI GCAATG 1 cut(s) 1756
BseMII CTCAG 9 cut(s) 1293, 2433, 2507, 2646, 3072, 3146, 3359, 3498, 3572
BseNI ACTGG 3 cut(s) 285, 1078, 1405
BseRI GAGGAG 3 cut(s) 376, 615, 1959
BseSI GKGCMC 1 cut(s) 318
BseXI GCAGC 7 cut(s) 193, 2355, 2568, 2781, 3207, 3420, 3633
BseYI CCCAGC 1 cut(s) 468
BsgI GTGCAG 1 cut(s) 848
Bsh1236I CGCG 2 cut(s) 208, 210
BshFI GGCC 1 cut(s) 396
BshNI GGYRCC 1 cut(s) 776
BsiHKAI GWGCWC 7 cut(s) 270, 1429, 1489, 2606, 3032, 3245, 3458
BsiHKCI CYCGRG 1 cut(s) 2186
BsiSI CCGG 1 cut(s) 465
BslI CCNNNNNNNGG 5 cut(s) 280, 1252, 1253, 1400, 1401
BsmAI GTCTC 6 cut(s) 1104, 1160, 1444, 1504, 1820, 1904
BsnI GGCC 1 cut(s) 396
Bso31I GGTCTC 1 cut(s) 1160
BsoBI CYCGRG 1 cut(s) 2186
Bsp119I TTCGAA 3 cut(s) 476, 2029, 2100
Bsp1286I GDGCHC 9 cut(s) 159, 270, 318, 1429, 1489, 2606, 3032, 3245, 3458
Bsp1407I TGTACA 2 cut(s) 1204, 2036
Bsp143I GATC 8 cut(s) 195, 292, 334, 540, 847, 1026, 1420, 2221
BspACI CCGC 7 cut(s) 349, 424, 438, 544, 1247, 1346, 1799
BspANI GGCC 1 cut(s) 396
BspCNI CTCAG 9 cut(s) 1294, 2434, 2508, 2647, 3073, 3147, 3360, 3499, 3573
BspFNI CGCG 2 cut(s) 208, 210
BspHI TCATGA 1 cut(s) 1261
BspLI GGNNCC 4 cut(s) 158, 336, 778, 1924
BspMI ACCTGC 1 cut(s) 75
BspPI GGATC 7 cut(s) 190, 300, 329, 342, 535, 855, 2229
BspT104I TTCGAA 3 cut(s) 476, 2029, 2100
BspT107I GGYRCC 1 cut(s) 776
BspTI CTTAAG 4 cut(s) 1705, 2117, 3038, 3251
BspTNI GGTCTC 1 cut(s) 1160
BsrBI CCGCTC 2 cut(s) 440, 1346
BsrDI GCAATG 1 cut(s) 1756
BsrGI TGTACA 2 cut(s) 1204, 2036
BsrI ACTGG 3 cut(s) 285, 1078, 1405
BssECI CCNNGG 3 cut(s) 38, 1038, 1580
BssMI GATC 8 cut(s) 195, 292, 334, 540, 847, 1026, 1420, 2221
BssNAI GTATAC 1 cut(s) 1702
BssT1I CCWWGG 2 cut(s) 38, 1038
Bst1107I GTATAC 1 cut(s) 1702
Bst2UI CCWGG 2 cut(s) 1581, 2084
Bst4CI ACNGT 5 cut(s) 1379, 1438, 1498, 1766, 1851
Bst6I CTCTTC 8 cut(s) 411, 1212, 2370, 2583, 2796, 3009, 3222, 3435
BstAFI CTTAAG 4 cut(s) 1705, 2117, 3038, 3251
BstAUI TGTACA 2 cut(s) 1204, 2036
BstBI TTCGAA 3 cut(s) 476, 2029, 2100
BstC8I GCNNGC 5 cut(s) 2481, 2907, 3120, 3333, 3546
BstF5I GGATG 6 cut(s) 496, 616, 2060, 2743, 2819, 2887
BstFNI CGCG 2 cut(s) 208, 210
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 8 cut(s) 198, 295, 337, 543, 850, 1029, 1423, 2224
BstMAI GTCTC 6 cut(s) 1104, 1160, 1444, 1504, 1820, 1904
BstMBI GATC 8 cut(s) 195, 292, 334, 540, 847, 1026, 1420, 2221
BstMWI GCNNNNNNNGC 9 cut(s) 11, 96, 1649, 1888, 2656, 2869, 3082, 3295, 3508
BstNI CCWGG 2 cut(s) 1581, 2084
BstNSI RCATGY 4 cut(s) 595, 1332, 1891, 3122
BstSCI CCNGG 2 cut(s) 1579, 2082
BstSFI CTRYAG 2 cut(s) 878, 1030
BstSLI GKGCMC 1 cut(s) 318
BstUI CGCG 2 cut(s) 208, 210
BstV1I GCAGC 7 cut(s) 193, 2355, 2568, 2781, 3207, 3420, 3633
BstV2I GAAGAC 7 cut(s) 81, 2327, 2540, 2753, 2966, 3392, 3605
BstX2I RGATCY 2 cut(s) 334, 2221
BstXI CCANNNNNNTGG 1 cut(s) 1933
BstYI RGATCY 2 cut(s) 334, 2221
BstZ17I GTATAC 1 cut(s) 1702
BsuRI GGCC 1 cut(s) 396
BtrI CACGTC 1 cut(s) 52
BtsCI GGATG 6 cut(s) 496, 616, 2060, 2743, 2819, 2887
BtsI GCAGTG 6 cut(s) 2460, 2673, 2886, 3099, 3312, 3525
BtsIMutI CAGTG 9 cut(s) 1771, 2235, 2460, 2673, 2886, 3099, 3152, 3312, 3525
BveI ACCTGC 1 cut(s) 75
Cac8I GCNNGC 5 cut(s) 2481, 2907, 3120, 3333, 3546
CaiI CAGNNNCTG 1 cut(s) 187
CciI TCATGA 1 cut(s) 1261
CfoI GCGC 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 435
CseI GACGC 4 cut(s) 2740, 2898, 3111, 3324
CsiI ACCWGGT 1 cut(s) 2082
Csp6I GTAC 5 cut(s) 782, 1007, 1139, 1205, 2037
CviQI GTAC 5 cut(s) 782, 1007, 1139, 1205, 2037
DpnI GATC 8 cut(s) 197, 294, 336, 542, 849, 1028, 1422, 2223
DpnII GATC 8 cut(s) 195, 292, 334, 540, 847, 1026, 1420, 2221
DraI TTTAAA 9 cut(s) 1339, 1788, 2241, 2493, 2706, 2919, 3132, 3345, 3558
DraIII CACNNNGTG 1 cut(s) 255
DrdI GACNNNNNNGTC 1 cut(s) 2863
DseDI GACNNNNNNGTC 1 cut(s) 2863
Eam1104I CTCTTC 8 cut(s) 411, 1212, 2370, 2583, 2796, 3009, 3222, 3435
EarI CTCTTC 8 cut(s) 411, 1212, 2370, 2583, 2796, 3009, 3222, 3435
Ecl136II GAGCTC 2 cut(s) 268, 1427
Eco130I CCWWGG 2 cut(s) 38, 1038
Eco24I GRGCYC 3 cut(s) 159, 270, 1429
Eco31I GGTCTC 1 cut(s) 1160
Eco47I GGWCC 1 cut(s) 435
Eco53kI GAGCTC 2 cut(s) 268, 1427
Eco57I CTGAAG 1 cut(s) 1997
Eco88I CYCGRG 1 cut(s) 2186
EcoICRI GAGCTC 2 cut(s) 268, 1427
EcoRI GAATTC 2 cut(s) 761, 1257
EcoRII CCWGG 2 cut(s) 1579, 2082
EcoT14I CCWWGG 2 cut(s) 38, 1038
EcoT22I ATGCAT 3 cut(s) 597, 1464, 1524
EcoT38I GRGCYC 3 cut(s) 159, 270, 1429
ErhI CCWWGG 2 cut(s) 38, 1038
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FauI CCCGC 1 cut(s) 1254
FauNDI CATATG 2 cut(s) 786, 1820
FblI GTMKAC 1 cut(s) 1701
FokI GGATG 6 cut(s) 503, 603, 2047, 2750, 2826, 2894
FriOI GRGCYC 3 cut(s) 159, 270, 1429
FspBI CTAG 8 cut(s) 693, 807, 993, 1004, 1226, 1292, 1472, 1532
GlaI GCGC 1 cut(s) 209
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 2 cut(s) 1971, 2033
HaeIII GGCC 1 cut(s) 396
HapII CCGG 1 cut(s) 465
HgaI GACGC 4 cut(s) 2740, 2898, 3111, 3324
HhaI GCGC 1 cut(s) 210
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HincII GTYRAC 1 cut(s) 916
HindII GTYRAC 1 cut(s) 916
HindIII AAGCTT 4 cut(s) 375, 729, 1279, 1716
HpaII CCGG 1 cut(s) 465
Hpy166II GTNNAC 4 cut(s) 916, 1702, 2045, 2056
Hpy8I GTNNAC 4 cut(s) 916, 1702, 2045, 2056
Hpy99I CGWCG 2 cut(s) 53, 641
HpyCH4III ACNGT 5 cut(s) 1379, 1438, 1498, 1766, 1851
HpyCH4IV ACGT 2 cut(s) 51, 639
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 96, 1649, 1888, 2656, 2869, 3082, 3295, 3508
HpySE526I ACGT 2 cut(s) 51, 639
HspAI GCGC 1 cut(s) 208
Kzo9I GATC 8 cut(s) 195, 292, 334, 540, 847, 1026, 1420, 2221
LmnI GCTCC 8 cut(s) 162, 265, 363, 628, 1016, 1058, 1343, 1922
Lsp1109I GCAGC 7 cut(s) 193, 2355, 2568, 2781, 3207, 3420, 3633
LweI GCATC 7 cut(s) 23, 136, 1012, 1449, 1509, 2804, 3557
MabI ACCWGGT 1 cut(s) 2082
MaeI CTAG 8 cut(s) 693, 807, 993, 1004, 1226, 1292, 1472, 1532
MaeII ACGT 2 cut(s) 51, 639
MalI GATC 8 cut(s) 197, 294, 336, 542, 849, 1028, 1422, 2223
MbiI CCGCTC 2 cut(s) 440, 1346
MboI GATC 8 cut(s) 195, 292, 334, 540, 847, 1026, 1420, 2221
MflI RGATCY 2 cut(s) 334, 2221
MhlI GDGCHC 9 cut(s) 159, 270, 318, 1429, 1489, 2606, 3032, 3245, 3458
MlyI GAGTC 4 cut(s) 2767, 2980, 3406, 3619
MmeI TCCRAC 1 cut(s) 631
Mph1103I ATGCAT 3 cut(s) 597, 1464, 1524
MroXI GAANNNNTTC 4 cut(s) 798, 1554, 1614, 2547
MslI CAYNNNNRTG 2 cut(s) 410, 594
MspA1I CMGCKG 1 cut(s) 883
MspCI CTTAAG 4 cut(s) 1705, 2117, 3038, 3251
MspI CCGG 1 cut(s) 465
MspR9I CCNGG 2 cut(s) 1581, 2084
MvaI CCWGG 2 cut(s) 1581, 2084
MvnI CGCG 2 cut(s) 208, 210
MwoI GCNNNNNNNGC 9 cut(s) 11, 96, 1649, 1888, 2656, 2869, 3082, 3295, 3508
NdeI CATATG 2 cut(s) 786, 1820
NdeII GATC 8 cut(s) 195, 292, 334, 540, 847, 1026, 1420, 2221
NlaIV GGNNCC 4 cut(s) 158, 336, 778, 1924
NmeAIII GCCGAG 1 cut(s) 409
NmuCI GTSAC 4 cut(s) 2351, 2990, 3203, 3634
NsiI ATGCAT 3 cut(s) 597, 1464, 1524
NspI RCATGY 4 cut(s) 595, 1332, 1891, 3122
NspV TTCGAA 3 cut(s) 476, 2029, 2100
PaeI GCATGC 1 cut(s) 3122
PaeR7I CTCGAG 1 cut(s) 2186
PagI TCATGA 1 cut(s) 1261
PaqCI CACCTGC 1 cut(s) 75
PdmI GAANNNNTTC 4 cut(s) 798, 1554, 1614, 2547
PfeI GAWTC 9 cut(s) 118, 203, 473, 584, 810, 978, 1298, 1565, 1870
PflMI CCANNNNNTGG 1 cut(s) 280
PleI GAGTC 4 cut(s) 2766, 2979, 3405, 3618
PpsI GAGTC 4 cut(s) 2766, 2979, 3405, 3618
PsiI TTATAA 2 cut(s) 1194, 1433
Psp124BI GAGCTC 2 cut(s) 270, 1429
Psp6I CCWGG 2 cut(s) 1579, 2082
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 2 cut(s) 1579, 2082
PspN4I GGNNCC 4 cut(s) 158, 336, 778, 1924
PspPI GGNCC 1 cut(s) 435
PstNI CAGNNNCTG 1 cut(s) 187
PsuI RGATCY 2 cut(s) 334, 2221
PvuII CAGCTG 1 cut(s) 883
RsaI GTAC 5 cut(s) 783, 1008, 1140, 1206, 2038
RsaNI GTAC 5 cut(s) 782, 1007, 1139, 1205, 2037
RseI CAYNNNNRTG 2 cut(s) 410, 594
SacI GAGCTC 2 cut(s) 270, 1429
Sau3AI GATC 8 cut(s) 195, 292, 334, 540, 847, 1026, 1420, 2221
Sau96I GGNCC 1 cut(s) 435
ScaI AGTACT 1 cut(s) 1008
SchI GAGTC 4 cut(s) 2767, 2980, 3406, 3619
ScrFI CCNGG 2 cut(s) 1581, 2084
SduI GDGCHC 9 cut(s) 159, 270, 318, 1429, 1489, 2606, 3032, 3245, 3458
SexAI ACCWGGT 1 cut(s) 2082
SfaNI GCATC 7 cut(s) 23, 136, 1012, 1449, 1509, 2804, 3557
SfcI CTRYAG 2 cut(s) 878, 1030
Sfr274I CTCGAG 1 cut(s) 2186
SfuI TTCGAA 3 cut(s) 476, 2029, 2100
SinI GGWCC 1 cut(s) 435
SlaI CTCGAG 1 cut(s) 2186
SmiI ATTTAAAT 1 cut(s) 2241
SmiMI CAYNNNNRTG 2 cut(s) 410, 594
SphI GCATGC 1 cut(s) 3122
SsiI CCGC 7 cut(s) 349, 424, 438, 544, 1247, 1346, 1799
SspI AATATT 1 cut(s) 2434
SspMI CTAG 8 cut(s) 693, 807, 993, 1004, 1226, 1292, 1472, 1532
SstI GAGCTC 2 cut(s) 270, 1429
StyD4I CCNGG 2 cut(s) 1579, 2082
StyI CCWWGG 2 cut(s) 38, 1038
SwaI ATTTAAAT 1 cut(s) 2241
TaaI ACNGT 5 cut(s) 1379, 1438, 1498, 1766, 1851
TaiI ACGT 2 cut(s) 54, 642
TatI WGTACW 3 cut(s) 1006, 1204, 2036
TfiI GAWTC 9 cut(s) 118, 203, 473, 584, 810, 978, 1298, 1565, 1870
TscAI CASTG 9 cut(s) 1771, 2235, 2467, 2680, 2893, 3106, 3159, 3319, 3532
TseFI GTSAC 4 cut(s) 2351, 2990, 3203, 3634
Tsp45I GTSAC 4 cut(s) 2351, 2990, 3203, 3634
TspGWI ACGGA 2 cut(s) 1661, 2012
TspRI CASTG 9 cut(s) 1771, 2235, 2467, 2680, 2893, 3106, 3159, 3319, 3532
Van91I CCANNNNNTGG 1 cut(s) 280
Vha464I CTTAAG 4 cut(s) 1705, 2117, 3038, 3251
VpaK11BI GGWCC 1 cut(s) 435
XbaI TCTAGA 1 cut(s) 806
XceI RCATGY 4 cut(s) 595, 1332, 1891, 3122
XcmI CCANNNNNNNNNTGG 1 cut(s) 1763
XhoI CTCGAG 1 cut(s) 2186
XmiI GTMKAC 1 cut(s) 1701
XmnI GAANNNNTTC 4 cut(s) 798, 1554, 1614, 2547
XspI CTAG 8 cut(s) 693, 807, 993, 1004, 1226, 1292, 1472, 1532
ZrmI AGTACT 1 cut(s) 1008
Zsp2I ATGCAT 3 cut(s) 597, 1464, 1524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.