Rroxscaffold_4G00284870

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000067
Physical Location & Seq
Reverse (-)
6385241 .. 6391265
6025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00284870.1

Sequence Viewer

Length: 3090 bp
ATGGCTGCTTCTTCGTCTCCCTCATCTGGTGGTGGTTGGAACTATGACGTGTTCCTCAATTTTAGAGGCGAGGATACTCGCAAGATCTTTGTAGGCCATCTCCACAAAGCTCTTGATCTGGAAGCAATCAACTCATTCATAGACTCTGAAGATCTCCGCAAAGGCAACGACCTTTCAGAGTTGCTCAGGGCTATTGAAGACTCTAGGCTTTCAATAGTAGTTTTCTCCCAAAACTATGCTTCCTCAACGTGGTGCTTGAAAGAACTGGTTAAGATACTGGAATGCATGTCTACAAAAAAGCAGCAGGTGGTGCCCATTTTTTATGAAGTGGATCCCTCTGATATCCGCAAAGTAAACGGAAGGTTTGCGGAGGCTTTTGCGAAACATGAGCAAGATTCTAATGCCGACATGGAAGAGGTGAATAGGTGGAAGATTGCTCTAACTGAAGCCAGCAATCTATCTGGCTGGGATTCACGAAATTACACGGATGATGCCAAGCTTATTGAGAAAATTGTTGAAGATATTTCTAAAAAATTGGTCCACATCCGATCCAATAAAGTAAGTAACTTGGTTGGAATGGACTCCCACATTGCGAAAATGAGTTCACTATTAGACCTTGGTGAGGACGATGTTCGCGTTGTTGGAGTATATGGAATGCCTGGTATAGGGAAAACAACCATTGCTAGAGCTATTTATGACGAACTTGTTTGTCAATTTGATCACTATTGCTTTCTTGAAAATGTCAAGGAAGGTTTCAAGCATAATGGTGCCATACATATGCAGGAAGAACTTCTATCTAGGATTTTTGAGAAAAGCGTGCGCAGTCTAGGCACTTTGAGTAGAGGTTCCAAGATGATAATGGATAGGCTGAGTAAGAGAAAAGTTCTGCTTGTTCTTGATGATGTAGAGAACTCTTACCAAATTGAAGAGTTACTTGGAAAGCAGCATTCATTTGGTAGCGGGAGTAGAATCATTGTAACCACTAGGGATATACAATCACTCAGTGGAGTTAATGCGAGATATAGTCCCATGTTTCTAAGCGATAGTGAAGCTCTTGAACTTTTTTTGCAGTATGCCTTCAGAACAAACCAACCTACGAGTGAGTATGATCCTCTCTCAAGGCGTGCTGTAGAATATTCTCAAGGTCTACCTTTAGCACTCAAAGTTTTGGGAGCTAGTCTTGATGGCAAAAGTATACGTGAGTGGGAAGATGAGCTAGAGAAAATAAAGGAAATCCCGCATAAGGAAATTCAGGGTGTGCTTAGAACAAGCTTTGATGGACTGGATCCTTCACAGCAGGAAATCTTTCTAGACATTGCATGTTTCTTTAGAGGAATGGACGAAGGCTATGTAATCAAAATTCTGGAAAATTGTGGCTTCTATCCCCACAGTGGATTACAAGTACTACGTGATAGAGCTCTCTTAACTATCTCATATGGCAATAGGCTTGAAATGCATGATTTAATACAGGATATGGGTTGGCAAATCGTCCGCCAACAATCTATTAAAGAGCCTGGGAAGCGCAGTCGTTTGTGGACTTATGAAGACGTGGATCGTGTATTAGCTCAAAATACGGCTACAGAAGCAGTTGAAGGCATAATGCTGGACTTGTCGAAGTCAAAAGACGTTGACATAGATGCTGAAGCTTTTGTTAGAATGACGAATCTAAGACTGCTCTTAATCGGTTATAACCACTACATAGACTTCAATGCGAAAGATTTTCCATTGGAGCTGTTACACCAGCCAAGAGATGAGTGTAAACAACACGTGAGTGGGGTTGTGAAGTTTCTTTCTCGTGACTTGAGGTATCTCATGTGGCATGGATGCCCCCTAAAGTCCTTGCCATCCAATTTTGGCCCGAAGAATCTTGTGGATCTTGACATGCGTTTTAGCCACATTGAACAACTTTGGGAAGGAATCAAGGGTGATTATCGACTTCTGCTTTCCTTTAATATGTCTCTTCCTGGAAGTGAGATTCCTGATTGGTTCAATTTTCAATGTAGGGGATCCTCATTTACTGTACAGCTACCCCCAAATTGGTTTGATAATAAGTTTTTGGGGTTCGCTATATGTGCTGTTGGCGACATCAAGGGTTCTCAGAATGATGCATCTGATCTATCCGCTCTATGTCATTGTAGCTTAAAAGGAAATCATGGTCAGTACAGTTTCAGTTCTACTTTGCTTGATTGGGGTTTCACTACTGATAGAATCCTCAAGTCGGATCATATGTTCATGGCATATGTGCCATGGTCTGAGTATCGCTTCATTGAAGAGGGAAAGCTGGTCAATGAACGGTATTTCACTGAGGCCACATTTGAGATTGTAGTAGAAAATAGAGTTGATAGACATGGTTTCGGGACAGTAATAAACCGCCATTGCATCACAAGTTGCGGAGTTCGTTTCTTTCATGGTGATTACATGGAGGAGCAAATTCATACCATCAGCGAAACCAAATCTCATAATGACAGCTGTGACATCCTTTCGGTTAGTAGGAAAGGAAAAGAGGGGTTGGGGTGCGGTTGGAGGAGTCTCCAGCGGAAGGCAGCTCTGCCGGTGAGAGCAAGAAGAGGCCGGGTTCAGAGGATCAGAAGGGTACCAACAAGCGAAGGTCAAAAGAGTCCAGAGTCATGGACGGTGGTTTCAAATACGCTTGATGATGGTCAGGTTTGGAGGAAGTATGGGCGGAAGGAAATACTGGGTTCTCCATATCCAAGGGTTTACTTCAGATGTACCCGCAAGTATGATCAAGGTTGCCAAGCAACCAAACGAGTCCAACAAGTCCAAGACACCCCAAAAGAGTACGAAATTACCTACAAAGGAAACCACACGTGCGTAAACATGACGATGATCATGGGAGCCTCCGATCCTGCTACTCGGCCGACCCCAATTTGCAGTAAGAAAGAACATCATGGCGATCTCTCTAGCTCACTGTCCCTGATTCCTTTCGTTCCGGAGAAGAAAGAAGAGTGCAAGGAGGGGACGACGGCGAGTGGTCTGGTGGACAACGTTAACGATACTGGTGATATGTGGACGGATTTTGAGTCGCCGGAGTTTGTGTTCCCGGATTTAGGGACTGAACAGTCACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1029

Amino Acids

117.15

Weight (kDa)

5.89

Isoelectric Point (pI)

46.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 14 - 191 6.3e-54 TIR domain
TIR_2 PF13676 17 - 116 5.2e-14 TIR domain
NB-ARC PF00931 200 - 363 3.4e-19 NB-ARC domain
WHD_ROQ1 PF23282 432 - 501 9.8e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 622 - 641 5.5e-07 Leucine Rich Repeat
C-JID PF20160 654 - 803 5.2e-21 C-JID domain
WRKY PF03106 885 - 944 2.3e-21 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1689
AarI CACCTGC 1 cut(s) 295
AasI GACNNNNNNGTC 1 cut(s) 3079
Acc16I TGCGCA 1 cut(s) 821
Acc36I ACCTGC 1 cut(s) 295
Acc65I GGTACC 1 cut(s) 2593
AccB1I GGYRCC 3 cut(s) 310, 767, 2593
AccBSI CCGCTC 1 cut(s) 2123
AccI GTMKAC 3 cut(s) 290, 1147, 1195
AccII CGCG 1 cut(s) 636
AccIII TCCGGA 1 cut(s) 2950
AclI AACGTT 1 cut(s) 3006
AcoI YGGCCR 1 cut(s) 2876
AcsI RAATTY 3 cut(s) 1248, 1359, 2430
AcuI CTGAAG 5 cut(s) 168, 465, 1063, 1662, 2707
AcvI CACGTG 2 cut(s) 1768, 2829
AdeI CACNNNGTG 1 cut(s) 1004
AfaI GTAC 6 cut(s) 1404, 2022, 2162, 2595, 2731, 2801
AflIII ACRYGT 3 cut(s) 48, 1765, 2826
AjiI CACGTC 2 cut(s) 49, 1549
AjnI CCWGG 3 cut(s) 658, 1513, 1963
AjuI GAANNNNNNNTTGG 2 cut(s) 918, 950
AleI CACNNNNGTG 1 cut(s) 1770
AloI GAACNNNNNNTCC 4 cut(s) 2213, 2245, 3041, 3073
Alw21I GWGCWC 1 cut(s) 1420
Alw26I GTCTC 3 cut(s) 21, 1962, 2534
Aor13HI TCCGGA 1 cut(s) 2950
AoxI GGCC 5 cut(s) 94, 1855, 2307, 2569, 2876
ApeKI GCWGC 4 cut(s) 5, 301, 943, 2543
ApoI RAATTY 3 cut(s) 1248, 1359, 2430
Asp700I GAANNNNTTC 2 cut(s) 789, 1305
Asp718I GGTACC 1 cut(s) 2593
AspLEI GCGC 2 cut(s) 822, 1524
AspS9I GGNCC 2 cut(s) 538, 1856
AsuC2I CCSGG 2 cut(s) 2573, 3062
AsuHPI GGTGA 7 cut(s) 430, 632, 1937, 2423, 2566, 3032, 3075
AvaII GGWCC 1 cut(s) 538
BaeGI GKGCMC 1 cut(s) 315
BamHI GGATCC 3 cut(s) 331, 1285, 2006
BanI GGYRCC 3 cut(s) 310, 767, 2593
BanII GRGCYC 1 cut(s) 1420
BauI CACGAG 1 cut(s) 1794
BbrPI CACGTG 2 cut(s) 1768, 2829
BbsI GAAGAC 2 cut(s) 204, 1551
Bbv12I GWGCWC 1 cut(s) 1420
BbvI GCAGC 3 cut(s) 313, 955, 2555
BccI CCATC 6 cut(s) 105, 1178, 1271, 1852, 2447, 2651
BceAI ACGGC 2 cut(s) 1590, 3000
BciT130I CCWGG 3 cut(s) 660, 1515, 1965
BciVI GTATCC 1 cut(s) 67
BclI TGATCA 3 cut(s) 718, 2743, 2847
BcnI CCSGG 2 cut(s) 2573, 3062
BcoDI GTCTC 3 cut(s) 21, 1962, 2534
BfaI CTAG 9 cut(s) 204, 684, 798, 827, 984, 1176, 1217, 1310, 2922
BfmI CTRYAG 2 cut(s) 1128, 1578
BfuAI ACCTGC 1 cut(s) 295
BfuI GTATCC 1 cut(s) 67
BglII AGATCT 2 cut(s) 84, 151
BisI GCNGC 4 cut(s) 6, 302, 944, 2544
BlsI GCNGC 4 cut(s) 7, 303, 945, 2545
BmcAI AGTACT 1 cut(s) 1404
Bme1390I CCNGG 5 cut(s) 660, 1515, 1965, 2573, 3062
Bme18I GGWCC 1 cut(s) 538
BmgBI CACGTC 2 cut(s) 49, 1549
BmgT120I GGNCC 2 cut(s) 538, 1856
BmiI GGNNCC 8 cut(s) 312, 333, 769, 847, 1287, 2008, 2595, 2857
BmrFI CCNGG 5 cut(s) 660, 1515, 1965, 2573, 3062
BmrI ACTGGG 1 cut(s) 2705
BmsI GCATC 6 cut(s) 481, 1627, 1814, 2095, 2117, 2388
BmuI ACTGGG 1 cut(s) 2705
BpiI GAAGAC 2 cut(s) 204, 1551
BpmI CTGGAG 1 cut(s) 2516
Bpu10I CCTNAGC 1 cut(s) 185
BpuEI CTTGAG 4 cut(s) 1102, 1125, 1822, 2198
BpuMI CCSGG 2 cut(s) 2573, 3062
BsaAI YACGTR 4 cut(s) 1199, 1409, 1768, 2829
BsaJI CCNNGG 4 cut(s) 616, 1514, 2246, 2711
BsaWI WCCGGW 1 cut(s) 2950
BsaXI ACNNNNNCTCC 2 cut(s) 3041, 3071
Bse118I RCCGGY 1 cut(s) 2551
Bse1I ACTGG 5 cut(s) 270, 282, 1287, 2700, 3022
Bse3DI GCAATG 4 cut(s) 588, 678, 1314, 2374
BseAI TCCGGA 1 cut(s) 2950
BseBI CCWGG 3 cut(s) 660, 1515, 1965
BseDI CCNNGG 4 cut(s) 616, 1514, 2246, 2711
BseGI GGATG 5 cut(s) 493, 543, 1829, 1844, 2475
BseMI GCAATG 4 cut(s) 588, 678, 1314, 2374
BseMII CTCAG 6 cut(s) 199, 860, 1015, 2111, 2244, 2295
BseNI ACTGG 5 cut(s) 270, 282, 1287, 2700, 3022
BseRI GAGGAG 2 cut(s) 2438, 2539
BseSI GKGCMC 1 cut(s) 315
BseX3I CGGCCG 1 cut(s) 2876
BseXI GCAGC 3 cut(s) 313, 955, 2555
BseYI CCCAGC 1 cut(s) 465
Bsh1236I CGCG 1 cut(s) 636
Bsh1285I CGRYCG 1 cut(s) 2879
BshFI GGCC 5 cut(s) 96, 1857, 2309, 2571, 2878
BshNI GGYRCC 3 cut(s) 310, 767, 2593
BsiEI CGRYCG 1 cut(s) 2879
BsiHKAI GWGCWC 1 cut(s) 1420
BsiSI CCGG 5 cut(s) 2552, 2572, 2951, 3047, 3062
BslFI GGGAC 5 cut(s) 1011, 2371, 2917, 2992, 3085
BsmAI GTCTC 3 cut(s) 21, 1962, 2534
BsmBI CGTCTC 1 cut(s) 21
BsmFI GGGAC 5 cut(s) 1011, 2371, 2917, 2992, 3085
BsmI GAATGC 3 cut(s) 287, 660, 946
BsnI GGCC 5 cut(s) 96, 1857, 2309, 2571, 2878
Bsp1286I GDGCHC 2 cut(s) 315, 1420
Bsp13I TCCGGA 1 cut(s) 2950
Bsp1407I TGTACA 1 cut(s) 2020
Bsp19I CCATGG 1 cut(s) 2246
BspANI GGCC 5 cut(s) 96, 1857, 2309, 2571, 2878
BspCNI CTCAG 6 cut(s) 198, 861, 1014, 2110, 2245, 2296
BspEI TCCGGA 1 cut(s) 2950
BspFNI CGCG 1 cut(s) 636
BspLI GGNNCC 8 cut(s) 312, 333, 769, 847, 1287, 2008, 2595, 2857
BspMI ACCTGC 1 cut(s) 295
BspT107I GGYRCC 3 cut(s) 310, 767, 2593
BsrBI CCGCTC 1 cut(s) 2123
BsrDI GCAATG 4 cut(s) 588, 678, 1314, 2374
BsrFI RCCGGY 1 cut(s) 2551
BsrGI TGTACA 1 cut(s) 2020
BsrI ACTGG 5 cut(s) 270, 282, 1287, 2700, 3022
BssAI RCCGGY 1 cut(s) 2551
BssECI CCNNGG 4 cut(s) 616, 1514, 2246, 2711
BssNAI GTATAC 1 cut(s) 1196
BssSI CACGAG 1 cut(s) 1794
BssT1I CCWWGG 3 cut(s) 616, 2246, 2711
Bst1107I GTATAC 1 cut(s) 1196
Bst2BI CACGAG 1 cut(s) 1794
Bst2UI CCWGG 3 cut(s) 660, 1515, 1965
Bst4CI ACNGT 8 cut(s) 1391, 2020, 2165, 2295, 2362, 2635, 2931, 3081
Bst6I CTCTTC 6 cut(s) 408, 921, 1965, 2265, 2560, 2958
BstAPI GCANNNNNTGC 1 cut(s) 310
BstAUI TGTACA 1 cut(s) 2020
BstBAI YACGTR 4 cut(s) 1199, 1409, 1768, 2829
BstC8I GCNNGC 3 cut(s) 451, 818, 1125
BstDEI CTNAG 9 cut(s) 185, 869, 1001, 1037, 1262, 1667, 2097, 2253, 2304
BstDSI CCRYGG 1 cut(s) 2246
BstENI CCTNNNNNAGG 2 cut(s) 620, 663
BstF5I GGATG 5 cut(s) 493, 543, 1829, 1844, 2475
BstFNI CGCG 1 cut(s) 636
BstHHI GCGC 2 cut(s) 822, 1524
BstMAI GTCTC 3 cut(s) 21, 1962, 2534
BstMCI CGRYCG 1 cut(s) 2879
BstMWI GCNNNNNNNGC 6 cut(s) 310, 828, 1453, 1519, 1583, 2072
BstNI CCWGG 3 cut(s) 660, 1515, 1965
BstNSI RCATGY 3 cut(s) 289, 1323, 1885
BstSCI CCNGG 5 cut(s) 658, 1513, 1963, 2571, 3060
BstSFI CTRYAG 2 cut(s) 1128, 1578
BstSLI GKGCMC 1 cut(s) 315
BstUI CGCG 1 cut(s) 636
BstV1I GCAGC 3 cut(s) 313, 955, 2555
BstV2I GAAGAC 2 cut(s) 204, 1551
BstX2I RGATCY 6 cut(s) 84, 151, 331, 1285, 1873, 2006
BstXI CCANNNNNNTGG 1 cut(s) 2628
BstYI RGATCY 6 cut(s) 84, 151, 331, 1285, 1873, 2006
BstZ17I GTATAC 1 cut(s) 1196
BstZI CGGCCG 1 cut(s) 2876
BsuI GTATCC 1 cut(s) 67
BsuRI GGCC 5 cut(s) 96, 1857, 2309, 2571, 2878
BtgI CCRYGG 1 cut(s) 2246
BtrI CACGTC 2 cut(s) 49, 1549
BtsCI GGATG 5 cut(s) 493, 543, 1829, 1844, 2475
BtsIMutI CAGTG 4 cut(s) 1009, 1396, 2301, 2927
BveI ACCTGC 1 cut(s) 295
Cac8I GCNNGC 3 cut(s) 451, 818, 1125
CfoI GCGC 2 cut(s) 822, 1524
Cfr10I RCCGGY 1 cut(s) 2551
Cfr13I GGNCC 2 cut(s) 538, 1856
Csp6I GTAC 6 cut(s) 1403, 2021, 2161, 2594, 2730, 2800
CviQI GTAC 6 cut(s) 1403, 2021, 2161, 2594, 2730, 2800
DdeI CTNAG 9 cut(s) 185, 869, 1001, 1037, 1262, 1667, 2097, 2253, 2304
DraIII CACNNNGTG 1 cut(s) 1004
DrdI GACNNNNNNGTC 1 cut(s) 3079
DseDI GACNNNNNNGTC 1 cut(s) 3079
EaeI YGGCCR 1 cut(s) 2876
EagI CGGCCG 1 cut(s) 2876
Eam1104I CTCTTC 6 cut(s) 408, 921, 1965, 2265, 2560, 2958
EarI CTCTTC 6 cut(s) 408, 921, 1965, 2265, 2560, 2958
EciI GGCGGA 2 cut(s) 1481, 2698
Ecl136II GAGCTC 1 cut(s) 1418
EclXI CGGCCG 1 cut(s) 2876
Eco130I CCWWGG 3 cut(s) 616, 2246, 2711
Eco24I GRGCYC 1 cut(s) 1420
Eco32I GATATC 1 cut(s) 343
Eco47I GGWCC 1 cut(s) 538
Eco52I CGGCCG 1 cut(s) 2876
Eco53kI GAGCTC 1 cut(s) 1418
Eco57I CTGAAG 5 cut(s) 168, 465, 1063, 1662, 2707
Eco72I CACGTG 2 cut(s) 1768, 2829
EcoICRI GAGCTC 1 cut(s) 1418
EcoNI CCTNNNNNAGG 2 cut(s) 620, 663
EcoRII CCWGG 3 cut(s) 658, 1513, 1963
EcoRV GATATC 1 cut(s) 343
EcoT14I CCWWGG 3 cut(s) 616, 2246, 2711
EcoT22I ATGCAT 3 cut(s) 287, 1458, 2110
EcoT38I GRGCYC 1 cut(s) 1420
ErhI CCWWGG 3 cut(s) 616, 2246, 2711
Esp3I CGTCTC 1 cut(s) 21
FalI AAGNNNNNCTT 4 cut(s) 873, 905, 918, 950
FaqI GGGAC 5 cut(s) 1011, 2371, 2917, 2992, 3085
FauI CCCGC 3 cut(s) 953, 1245, 2741
FauNDI CATATG 4 cut(s) 777, 1435, 2226, 2239
FbaI TGATCA 3 cut(s) 718, 2743, 2847
FblI GTMKAC 3 cut(s) 290, 1147, 1195
Fnu4HI GCNGC 4 cut(s) 6, 302, 944, 2544
FokI GGATG 5 cut(s) 500, 530, 1831, 1836, 2462
FriOI GRGCYC 1 cut(s) 1420
Fsp4HI GCNGC 4 cut(s) 6, 302, 944, 2544
FspBI CTAG 9 cut(s) 204, 684, 798, 827, 984, 1176, 1217, 1310, 2922
FspI TGCGCA 1 cut(s) 821
GlaI GCGC 2 cut(s) 821, 1523
GluI GCNGC 4 cut(s) 6, 302, 944, 2544
GsaI CCCAGC 1 cut(s) 469
GsuI CTGGAG 1 cut(s) 2516
HaeIII GGCC 5 cut(s) 96, 1857, 2309, 2571, 2878
HapII CCGG 5 cut(s) 2552, 2572, 2951, 3047, 3062
HhaI GCGC 2 cut(s) 822, 1524
Hin6I GCGC 2 cut(s) 820, 1522
HinP1I GCGC 2 cut(s) 820, 1522
HincII GTYRAC 2 cut(s) 1630, 3010
HindII GTYRAC 2 cut(s) 1630, 3010
HindIII AAGCTT 3 cut(s) 497, 1270, 1644
HpaI GTTAAC 1 cut(s) 3010
HpaII CCGG 5 cut(s) 2552, 2572, 2951, 3047, 3062
HphI GGTGA 7 cut(s) 430, 632, 1937, 2423, 2566, 3032, 3075
Hpy99I CGWCG 1 cut(s) 2986
HpyCH4III ACNGT 8 cut(s) 1391, 2020, 2165, 2295, 2362, 2635, 2931, 3081
HpyCH4IV ACGT 9 cut(s) 48, 248, 1198, 1408, 1548, 1626, 1767, 2828, 3006
HpyCH4V TGCA 9 cut(s) 285, 781, 1069, 1319, 1456, 2108, 2379, 2892, 2970
HpyF10VI GCNNNNNNNGC 6 cut(s) 310, 828, 1453, 1519, 1583, 2072
HpyF3I CTNAG 9 cut(s) 185, 869, 1001, 1037, 1262, 1667, 2097, 2253, 2304
HpySE526I ACGT 9 cut(s) 48, 248, 1198, 1408, 1548, 1626, 1767, 2828, 3006
HspAI GCGC 2 cut(s) 820, 1522
Kpn2I TCCGGA 1 cut(s) 2950
KpnI GGTACC 1 cut(s) 2597
Ksp22I TGATCA 3 cut(s) 718, 2743, 2847
KspAI GTTAAC 1 cut(s) 3010
LmnI GCTCC 4 cut(s) 1172, 1729, 2425, 2855
Lsp1109I GCAGC 3 cut(s) 313, 955, 2555
LweI GCATC 6 cut(s) 481, 1627, 1814, 2095, 2117, 2388
MaeI CTAG 9 cut(s) 204, 684, 798, 827, 984, 1176, 1217, 1310, 2922
MaeII ACGT 9 cut(s) 48, 248, 1198, 1408, 1548, 1626, 1767, 2828, 3006
MaeIII GTNAC 7 cut(s) 563, 930, 976, 1734, 1796, 2471, 3081
MbiI CCGCTC 1 cut(s) 2123
MflI RGATCY 6 cut(s) 84, 151, 331, 1285, 1873, 2006
MhlI GDGCHC 2 cut(s) 315, 1420
MlyI GAGTC 8 cut(s) 137, 194, 575, 2536, 2626, 2633, 2778, 3050
MmeI TCCRAC 6 cut(s) 17, 553, 622, 2199, 2501, 2797
Mph1103I ATGCAT 3 cut(s) 287, 1458, 2110
MroI TCCGGA 1 cut(s) 2950
MroXI GAANNNNTTC 2 cut(s) 789, 1305
MseI TTAA 9 cut(s) 270, 1011, 1424, 1463, 1506, 1679, 1950, 2141, 3009
MslI CAYNNNNRTG 4 cut(s) 765, 776, 1770, 2843
MspA1I CMGCKG 2 cut(s) 2469, 2536
MspI CCGG 5 cut(s) 2552, 2572, 2951, 3047, 3062
MspR9I CCNGG 5 cut(s) 660, 1515, 1965, 2573, 3062
Mva1269I GAATGC 3 cut(s) 287, 660, 946
MvaI CCWGG 3 cut(s) 660, 1515, 1965
MvnI CGCG 1 cut(s) 636
MwoI GCNNNNNNNGC 6 cut(s) 310, 828, 1453, 1519, 1583, 2072
NciI CCSGG 2 cut(s) 2573, 3062
NcoI CCATGG 1 cut(s) 2246
NdeI CATATG 4 cut(s) 777, 1435, 2226, 2239
NlaIV GGNNCC 8 cut(s) 312, 333, 769, 847, 1287, 2008, 2595, 2857
NmeAIII GCCGAG 1 cut(s) 2854
NmuCI GTSAC 3 cut(s) 1796, 2471, 3081
NsbI TGCGCA 1 cut(s) 821
NsiI ATGCAT 3 cut(s) 287, 1458, 2110
NspI RCATGY 3 cut(s) 289, 1323, 1885
OliI CACNNNNGTG 1 cut(s) 1770
PaqCI CACCTGC 1 cut(s) 295
PcsI WCGNNNNNNNCGW 1 cut(s) 633
PctI GAATGC 3 cut(s) 287, 660, 946
PdmI GAANNNNTTC 2 cut(s) 789, 1305
PfeI GAWTC 9 cut(s) 395, 470, 969, 1663, 1864, 1917, 1975, 2208, 2938
PfoI TCCNGGA 2 cut(s) 1963, 3060
PkrI GCNGC 4 cut(s) 7, 303, 945, 2545
PleI GAGTC 8 cut(s) 137, 194, 575, 2535, 2625, 2632, 2777, 3049
PmaCI CACGTG 2 cut(s) 1768, 2829
PmlI CACGTG 2 cut(s) 1768, 2829
PpsI GAGTC 8 cut(s) 137, 194, 575, 2535, 2625, 2632, 2777, 3049
Ppu21I YACGTR 4 cut(s) 1199, 1409, 1768, 2829
PsiI TTATAA 1 cut(s) 1689
Psp124BI GAGCTC 1 cut(s) 1420
Psp1406I AACGTT 1 cut(s) 3006
Psp6I CCWGG 3 cut(s) 658, 1513, 1963
PspCI CACGTG 2 cut(s) 1768, 2829
PspFI CCCAGC 1 cut(s) 465
PspGI CCWGG 3 cut(s) 658, 1513, 1963
PspN4I GGNNCC 8 cut(s) 312, 333, 769, 847, 1287, 2008, 2595, 2857
PspPI GGNCC 2 cut(s) 538, 1856
PsuI RGATCY 6 cut(s) 84, 151, 331, 1285, 1873, 2006
PvuII CAGCTG 1 cut(s) 2469
RsaI GTAC 6 cut(s) 1404, 2022, 2162, 2595, 2731, 2801
RsaNI GTAC 6 cut(s) 1403, 2021, 2161, 2594, 2730, 2800
RseI CAYNNNNRTG 4 cut(s) 765, 776, 1770, 2843
SacI GAGCTC 1 cut(s) 1420
SaqAI TTAA 9 cut(s) 270, 1011, 1424, 1463, 1506, 1679, 1950, 2141, 3009
SatI GCNGC 4 cut(s) 6, 302, 944, 2544
Sau96I GGNCC 2 cut(s) 538, 1856
ScaI AGTACT 1 cut(s) 1404
SchI GAGTC 8 cut(s) 137, 194, 575, 2536, 2626, 2633, 2778, 3050
ScrFI CCNGG 5 cut(s) 660, 1515, 1965, 2573, 3062
SduI GDGCHC 2 cut(s) 315, 1420
SfaNI GCATC 6 cut(s) 481, 1627, 1814, 2095, 2117, 2388
SfcI CTRYAG 2 cut(s) 1128, 1578
SinI GGWCC 1 cut(s) 538
SmiMI CAYNNNNRTG 4 cut(s) 765, 776, 1770, 2843
SmlI CTYRAG 4 cut(s) 1117, 1140, 1801, 2213
SmoI CTYRAG 4 cut(s) 1117, 1140, 1801, 2213
SspI AATATT 1 cut(s) 1136
SspMI CTAG 9 cut(s) 204, 684, 798, 827, 984, 1176, 1217, 1310, 2922
SstI GAGCTC 1 cut(s) 1420
StyD4I CCNGG 5 cut(s) 658, 1513, 1963, 2571, 3060
StyI CCWWGG 3 cut(s) 616, 2246, 2711
TaaI ACNGT 8 cut(s) 1391, 2020, 2165, 2295, 2362, 2635, 2931, 3081
TaiI ACGT 9 cut(s) 51, 251, 1201, 1411, 1551, 1629, 1770, 2831, 3009
TaqI TCGA 2 cut(s) 1613, 1933
TatI WGTACW 3 cut(s) 1402, 2020, 2160
TfiI GAWTC 9 cut(s) 395, 470, 969, 1663, 1864, 1917, 1975, 2208, 2938
Tru1I TTAA 9 cut(s) 270, 1011, 1424, 1463, 1506, 1679, 1950, 2141, 3009
Tru9I TTAA 9 cut(s) 270, 1011, 1424, 1463, 1506, 1679, 1950, 2141, 3009
TscAI CASTG 4 cut(s) 1009, 1396, 2308, 2934
TseFI GTSAC 3 cut(s) 1796, 2471, 3081
TseI GCWGC 4 cut(s) 5, 301, 943, 2543
Tsp45I GTSAC 3 cut(s) 1796, 2471, 3081
TspDTI ATGAA 9 cut(s) 127, 339, 939, 1557, 2221, 2254, 2304, 2396, 2423
TspGWI ACGGA 3 cut(s) 372, 500, 3047
TspRI CASTG 4 cut(s) 1009, 1396, 2308, 2934
VpaK11BI GGWCC 1 cut(s) 538
XagI CCTNNNNNAGG 2 cut(s) 620, 663
XapI RAATTY 3 cut(s) 1248, 1359, 2430
XbaI TCTAGA 1 cut(s) 1309
XceI RCATGY 3 cut(s) 289, 1323, 1885
XcmI CCANNNNNNNNNTGG 1 cut(s) 856
XmiI GTMKAC 3 cut(s) 290, 1147, 1195
XmnI GAANNNNTTC 2 cut(s) 789, 1305
XspI CTAG 9 cut(s) 204, 684, 798, 827, 984, 1176, 1217, 1310, 2922
ZrmI AGTACT 1 cut(s) 1404
Zsp2I ATGCAT 3 cut(s) 287, 1458, 2110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.