RESISTANCE protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 971, 1545 |
| AatII | GACGTC | 1 cut(s) | 132 |
| AccB1I | GGYRCC | 1 cut(s) | 307 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 304 |
| AccI | GTMKAC | 2 cut(s) | 1447, 1494 |
| AccIII | TCCGGA | 2 cut(s) | 23, 267 |
| AciI | CCGC | 2 cut(s) | 447, 1614 |
| AclWI | GGATC | 6 cut(s) | 278, 323, 529, 825, 1589, 1602 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcsI | RAATTY | 2 cut(s) | 801, 876 |
| AcuI | CTGAAG | 2 cut(s) | 827, 1530 |
| AcyI | GRCGYC | 1 cut(s) | 129 |
| AdeI | CACNNNGTG | 2 cut(s) | 249, 551 |
| AfaI | GTAC | 3 cut(s) | 774, 1188, 1263 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 274, 304, 947 |
| AflII | CTTAAG | 1 cut(s) | 350 |
| AflIII | ACRYGT | 1 cut(s) | 74 |
| AgsI | TTSAA | 7 cut(s) | 184, 194, 695, 716, 785, 1049, 1072 |
| AjiI | CACGTC | 1 cut(s) | 1236 |
| Alw26I | GTCTC | 4 cut(s) | 38, 1242, 1385, 1438 |
| AlwI | GGATC | 6 cut(s) | 278, 323, 529, 825, 1589, 1602 |
| Ama87I | CYCGRG | 2 cut(s) | 199, 1388 |
| Aor13HI | TCCGGA | 2 cut(s) | 23, 267 |
| AoxI | GGCC | 1 cut(s) | 3 |
| ApeKI | GCWGC | 2 cut(s) | 104, 504 |
| ApoI | RAATTY | 2 cut(s) | 801, 876 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1334, 1338, 1366, 1370 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 391, 577, 789 |
| AspLEI | GCGC | 1 cut(s) | 178 |
| AspS9I | GGNCC | 2 cut(s) | 265, 910 |
| AsuHPI | GGTGA | 2 cut(s) | 537, 779 |
| AvaI | CYCGRG | 2 cut(s) | 199, 1388 |
| AvaII | GGWCC | 2 cut(s) | 265, 910 |
| BaeGI | GKGCMC | 1 cut(s) | 312 |
| BamHI | GGATCC | 1 cut(s) | 1594 |
| BanI | GGYRCC | 1 cut(s) | 307 |
| BanII | GRGCYC | 2 cut(s) | 1020, 1626 |
| BauI | CACGAG | 2 cut(s) | 366, 721 |
| BbsI | GAAGAC | 4 cut(s) | 75, 124, 522, 1130 |
| BbvI | GCAGC | 2 cut(s) | 116, 516 |
| BccI | CCATC | 4 cut(s) | 102, 554, 827, 1274 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 155, 189 |
| BcoDI | GTCTC | 4 cut(s) | 38, 1242, 1385, 1438 |
| BfaI | CTAG | 5 cut(s) | 681, 863, 995, 1088, 1313 |
| BfmI | CTRYAG | 1 cut(s) | 1451 |
| BfrI | CTTAAG | 1 cut(s) | 350 |
| BglII | AGATCT | 2 cut(s) | 1418, 1513 |
| BisI | GCNGC | 3 cut(s) | 105, 447, 505 |
| BlsI | GCNGC | 3 cut(s) | 106, 448, 506 |
| Bme18I | GGWCC | 2 cut(s) | 265, 910 |
| BmeT110I | CYCGRG | 2 cut(s) | 199, 1388 |
| BmgBI | CACGTC | 1 cut(s) | 1236 |
| BmgT120I | GGNCC | 2 cut(s) | 265, 910 |
| BmiI | GGNNCC | 3 cut(s) | 309, 1596, 1623 |
| BmsI | GCATC | 3 cut(s) | 245, 669, 1446 |
| BpiI | GAAGAC | 4 cut(s) | 75, 124, 522, 1130 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 18, 50 |
| BpmI | CTGGAG | 1 cut(s) | 1640 |
| BpuEI | CTTGAG | 1 cut(s) | 1093 |
| BsaAI | YACGTR | 1 cut(s) | 77 |
| BsaBI | GATNNNNATC | 1 cut(s) | 539 |
| BsaHI | GRCGYC | 1 cut(s) | 129 |
| BsaWI | WCCGGW | 2 cut(s) | 23, 267 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 274, 304, 947 |
| Bse118I | RCCGGY | 1 cut(s) | 5 |
| Bse1I | ACTGG | 3 cut(s) | 267, 432, 1399 |
| Bse8I | GATNNNNATC | 1 cut(s) | 539 |
| BseAI | TCCGGA | 2 cut(s) | 23, 267 |
| BseGI | GGATG | 4 cut(s) | 20, 490, 628, 838 |
| BseJI | GATNNNNATC | 1 cut(s) | 539 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 274, 304, 947 |
| BseMII | CTCAG | 1 cut(s) | 1310 |
| BseNI | ACTGG | 3 cut(s) | 267, 432, 1399 |
| BseSI | GKGCMC | 1 cut(s) | 312 |
| BseXI | GCAGC | 2 cut(s) | 116, 516 |
| BseYI | CCCAGC | 1 cut(s) | 492 |
| BsgI | GTGCAG | 1 cut(s) | 1137 |
| BshFI | GGCC | 1 cut(s) | 5 |
| BshNI | GGYRCC | 1 cut(s) | 307 |
| BsiHKCI | CYCGRG | 2 cut(s) | 199, 1388 |
| BsiSI | CCGG | 3 cut(s) | 6, 24, 268 |
| BslFI | GGGAC | 1 cut(s) | 1499 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 274, 304, 947 |
| BsmAI | GTCTC | 4 cut(s) | 38, 1242, 1385, 1438 |
| BsmBI | CGTCTC | 1 cut(s) | 1242 |
| BsmFI | GGGAC | 1 cut(s) | 1499 |
| BsmI | GAATGC | 1 cut(s) | 1527 |
| BsnI | GGCC | 1 cut(s) | 5 |
| BsoBI | CYCGRG | 2 cut(s) | 199, 1388 |
| Bsp1286I | GDGCHC | 3 cut(s) | 312, 1020, 1626 |
| Bsp13I | TCCGGA | 2 cut(s) | 23, 267 |
| BspACI | CCGC | 2 cut(s) | 447, 1614 |
| BspANI | GGCC | 1 cut(s) | 5 |
| BspCNI | CTCAG | 1 cut(s) | 1309 |
| BspEI | TCCGGA | 2 cut(s) | 23, 267 |
| BspHI | TCATGA | 1 cut(s) | 1366 |
| BspLI | GGNNCC | 3 cut(s) | 309, 1596, 1623 |
| BspPI | GGATC | 6 cut(s) | 278, 323, 529, 825, 1589, 1602 |
| BspQI | GCTCTTC | 1 cut(s) | 141 |
| BspT107I | GGYRCC | 1 cut(s) | 307 |
| BspTI | CTTAAG | 1 cut(s) | 350 |
| BsrFI | RCCGGY | 1 cut(s) | 5 |
| BsrI | ACTGG | 3 cut(s) | 267, 432, 1399 |
| BssAI | RCCGGY | 1 cut(s) | 5 |
| BssNAI | GTATAC | 1 cut(s) | 1448 |
| BssNI | GRCGYC | 1 cut(s) | 129 |
| BssSI | CACGAG | 2 cut(s) | 366, 721 |
| Bst1107I | GTATAC | 1 cut(s) | 1448 |
| Bst2BI | CACGAG | 2 cut(s) | 366, 721 |
| Bst4CI | ACNGT | 4 cut(s) | 231, 612, 1186, 1432 |
| Bst6I | CTCTTC | 2 cut(s) | 141, 405 |
| BstACI | GRCGYC | 1 cut(s) | 129 |
| BstAFI | CTTAAG | 1 cut(s) | 350 |
| BstBAI | YACGTR | 1 cut(s) | 77 |
| BstC8I | GCNNGC | 3 cut(s) | 1116, 1273, 1616 |
| BstDEI | CTNAG | 2 cut(s) | 152, 1296 |
| BstF5I | GGATG | 4 cut(s) | 20, 490, 628, 838 |
| BstHHI | GCGC | 1 cut(s) | 178 |
| BstMAI | GTCTC | 4 cut(s) | 38, 1242, 1385, 1438 |
| BstNSI | RCATGY | 1 cut(s) | 1326 |
| BstSFI | CTRYAG | 1 cut(s) | 1451 |
| BstSLI | GKGCMC | 1 cut(s) | 312 |
| BstV1I | GCAGC | 2 cut(s) | 116, 516 |
| BstV2I | GAAGAC | 4 cut(s) | 75, 124, 522, 1130 |
| BstX2I | RGATCY | 5 cut(s) | 270, 328, 1418, 1513, 1594 |
| BstYI | RGATCY | 5 cut(s) | 270, 328, 1418, 1513, 1594 |
| BstZ17I | GTATAC | 1 cut(s) | 1448 |
| BsuRI | GGCC | 1 cut(s) | 5 |
| BtrI | CACGTC | 1 cut(s) | 1236 |
| BtsCI | GGATG | 4 cut(s) | 20, 490, 628, 838 |
| Cac8I | GCNNGC | 3 cut(s) | 1116, 1273, 1616 |
| CciI | TCATGA | 1 cut(s) | 1366 |
| CfoI | GCGC | 1 cut(s) | 178 |
| Cfr10I | RCCGGY | 1 cut(s) | 5 |
| Cfr13I | GGNCC | 2 cut(s) | 265, 910 |
| Csp6I | GTAC | 3 cut(s) | 773, 1187, 1262 |
| CviAII | CATG | 8 cut(s) | 246, 383, 586, 1289, 1323, 1367, 1460, 1587 |
| CviQI | GTAC | 3 cut(s) | 773, 1187, 1262 |
| DdeI | CTNAG | 2 cut(s) | 152, 1296 |
| DraI | TTTAAA | 2 cut(s) | 438, 1333 |
| DraIII | CACNNNGTG | 2 cut(s) | 249, 551 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| Eam1104I | CTCTTC | 2 cut(s) | 141, 405 |
| EarI | CTCTTC | 2 cut(s) | 141, 405 |
| Eco24I | GRGCYC | 2 cut(s) | 1020, 1626 |
| Eco47I | GGWCC | 2 cut(s) | 265, 910 |
| Eco57I | CTGAAG | 2 cut(s) | 827, 1530 |
| Eco88I | CYCGRG | 2 cut(s) | 199, 1388 |
| EcoRI | GAATTC | 2 cut(s) | 801, 876 |
| EcoT22I | ATGCAT | 2 cut(s) | 238, 1461 |
| EcoT38I | GRGCYC | 2 cut(s) | 1020, 1626 |
| Esp3I | CGTCTC | 1 cut(s) | 1242 |
| FaeI | CATG | 8 cut(s) | 249, 386, 589, 1292, 1326, 1370, 1463, 1590 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 734, 766, 1029, 1061 |
| FaqI | GGGAC | 1 cut(s) | 1499 |
| FatI | CATG | 8 cut(s) | 245, 382, 585, 1288, 1322, 1366, 1459, 1586 |
| FauI | CCCGC | 1 cut(s) | 1607 |
| FblI | GTMKAC | 2 cut(s) | 1447, 1494 |
| Fnu4HI | GCNGC | 3 cut(s) | 105, 447, 505 |
| FokI | GGATG | 4 cut(s) | 7, 497, 635, 845 |
| FriOI | GRGCYC | 2 cut(s) | 1020, 1626 |
| Fsp4HI | GCNGC | 3 cut(s) | 105, 447, 505 |
| FspBI | CTAG | 5 cut(s) | 681, 863, 995, 1088, 1313 |
| GlaI | GCGC | 1 cut(s) | 177 |
| GluI | GCNGC | 3 cut(s) | 105, 447, 505 |
| GsaI | CCCAGC | 1 cut(s) | 496 |
| GsuI | CTGGAG | 1 cut(s) | 1640 |
| HaeIII | GGCC | 1 cut(s) | 5 |
| HapII | CCGG | 3 cut(s) | 6, 24, 268 |
| HhaI | GCGC | 1 cut(s) | 178 |
| Hin1I | GRCGYC | 1 cut(s) | 129 |
| Hin1II | CATG | 8 cut(s) | 249, 386, 589, 1292, 1326, 1370, 1463, 1590 |
| Hin6I | GCGC | 1 cut(s) | 176 |
| HinP1I | GCGC | 1 cut(s) | 176 |
| HincII | GTYRAC | 2 cut(s) | 672, 1177 |
| HindII | GTYRAC | 2 cut(s) | 672, 1177 |
| HinfI | GANTC | 6 cut(s) | 140, 197, 578, 599, 642, 1292 |
| HpaI | GTTAAC | 1 cut(s) | 1177 |
| HpaII | CCGG | 3 cut(s) | 6, 24, 268 |
| HphI | GGTGA | 2 cut(s) | 537, 779 |
| Hpy166II | GTNNAC | 7 cut(s) | 672, 901, 1029, 1177, 1262, 1448, 1495 |
| Hpy188I | TCNGA | 6 cut(s) | 62, 111, 145, 528, 807, 1631 |
| Hpy8I | GTNNAC | 7 cut(s) | 672, 901, 1029, 1177, 1262, 1448, 1495 |
| Hpy99I | CGWCG | 3 cut(s) | 47, 131, 167 |
| HpyAV | CCTTC | 2 cut(s) | 736, 802 |
| HpyCH4III | ACNGT | 4 cut(s) | 231, 612, 1186, 1432 |
| HpyCH4IV | ACGT | 5 cut(s) | 45, 76, 129, 944, 1235 |
| HpyCH4V | TGCA | 7 cut(s) | 236, 365, 377, 1118, 1288, 1322, 1459 |
| HpyF3I | CTNAG | 2 cut(s) | 152, 1296 |
| HpySE526I | ACGT | 5 cut(s) | 45, 76, 129, 944, 1235 |
| Hsp92I | GRCGYC | 1 cut(s) | 129 |
| Hsp92II | CATG | 8 cut(s) | 249, 386, 589, 1292, 1326, 1370, 1463, 1590 |
| HspAI | GCGC | 1 cut(s) | 176 |
| Kpn2I | TCCGGA | 2 cut(s) | 23, 267 |
| KspAI | GTTAAC | 1 cut(s) | 1177 |
| LguI | GCTCTTC | 1 cut(s) | 141 |
| LmnI | GCTCC | 3 cut(s) | 957, 1163, 1621 |
| Lsp1109I | GCAGC | 2 cut(s) | 116, 516 |
| LweI | GCATC | 3 cut(s) | 245, 669, 1446 |
| MaeI | CTAG | 5 cut(s) | 681, 863, 995, 1088, 1313 |
| MaeII | ACGT | 5 cut(s) | 45, 76, 129, 944, 1235 |
| MaeIII | GTNAC | 4 cut(s) | 990, 1094, 1354, 1426 |
| MflI | RGATCY | 5 cut(s) | 270, 328, 1418, 1513, 1594 |
| MhlI | GDGCHC | 3 cut(s) | 312, 1020, 1626 |
| MlyI | GAGTC | 1 cut(s) | 191 |
| MmeI | TCCRAC | 3 cut(s) | 396, 619, 1188 |
| MnlI | CCTC | 9 cut(s) | 20, 56, 74, 195, 343, 739, 830, 846, 1397 |
| Mph1103I | ATGCAT | 2 cut(s) | 238, 1461 |
| MroI | TCCGGA | 2 cut(s) | 23, 267 |
| MroXI | GAANNNNTTC | 3 cut(s) | 391, 577, 789 |
| MseI | TTAA | 6 cut(s) | 99, 254, 351, 437, 1176, 1332 |
| MspCI | CTTAAG | 1 cut(s) | 350 |
| MspI | CCGG | 3 cut(s) | 6, 24, 268 |
| Mva1269I | GAATGC | 1 cut(s) | 1527 |
| NlaIII | CATG | 8 cut(s) | 249, 386, 589, 1292, 1326, 1370, 1463, 1590 |
| NlaIV | GGNNCC | 3 cut(s) | 309, 1596, 1623 |
| NmuCI | GTSAC | 1 cut(s) | 1354 |
| NsiI | ATGCAT | 2 cut(s) | 238, 1461 |
| NspI | RCATGY | 1 cut(s) | 1326 |
| PaeR7I | CTCGAG | 2 cut(s) | 199, 1388 |
| PagI | TCATGA | 1 cut(s) | 1366 |
| PciSI | GCTCTTC | 1 cut(s) | 141 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 206 |
| PctI | GAATGC | 1 cut(s) | 1527 |
| PdmI | GAANNNNTTC | 3 cut(s) | 391, 577, 789 |
| PfeI | GAWTC | 5 cut(s) | 140, 578, 599, 642, 1292 |
| PflFI | GACNNNGTC | 1 cut(s) | 1490 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 304 |
| PkrI | GCNGC | 3 cut(s) | 106, 448, 506 |
| PleI | GAGTC | 1 cut(s) | 191 |
| PpsI | GAGTC | 1 cut(s) | 191 |
| Ppu21I | YACGTR | 1 cut(s) | 77 |
| PsiI | TTATAA | 2 cut(s) | 971, 1545 |
| PspFI | CCCAGC | 1 cut(s) | 492 |
| PspN4I | GGNNCC | 3 cut(s) | 309, 1596, 1623 |
| PspPI | GGNCC | 2 cut(s) | 265, 910 |
| PspXI | VCTCGAGB | 1 cut(s) | 199 |
| PsuI | RGATCY | 5 cut(s) | 270, 328, 1418, 1513, 1594 |
| PsyI | GACNNNGTC | 1 cut(s) | 1490 |
| RsaI | GTAC | 3 cut(s) | 774, 1188, 1263 |
| RsaNI | GTAC | 3 cut(s) | 773, 1187, 1262 |
| SapI | GCTCTTC | 1 cut(s) | 141 |
| SaqAI | TTAA | 6 cut(s) | 99, 254, 351, 437, 1176, 1332 |
| SatI | GCNGC | 3 cut(s) | 105, 447, 505 |
| Sau96I | GGNCC | 2 cut(s) | 265, 910 |
| SchI | GAGTC | 1 cut(s) | 191 |
| SduI | GDGCHC | 3 cut(s) | 312, 1020, 1626 |
| SfaNI | GCATC | 3 cut(s) | 245, 669, 1446 |
| SfcI | CTRYAG | 1 cut(s) | 1451 |
| Sfr274I | CTCGAG | 2 cut(s) | 199, 1388 |
| SinI | GGWCC | 2 cut(s) | 265, 910 |
| SlaI | CTCGAG | 2 cut(s) | 199, 1388 |
| SmlI | CTYRAG | 4 cut(s) | 199, 350, 1108, 1388 |
| SmoI | CTYRAG | 4 cut(s) | 199, 350, 1108, 1388 |
| SsiI | CCGC | 2 cut(s) | 447, 1614 |
| SspI | AATATT | 2 cut(s) | 1252, 1308 |
| SspMI | CTAG | 5 cut(s) | 681, 863, 995, 1088, 1313 |
| TaaI | ACNGT | 4 cut(s) | 231, 612, 1186, 1432 |
| TaiI | ACGT | 5 cut(s) | 48, 79, 132, 947, 1238 |
| TaqI | TCGA | 6 cut(s) | 126, 200, 209, 402, 820, 1389 |
| TatI | WGTACW | 2 cut(s) | 772, 1261 |
| TauI | GCSGC | 1 cut(s) | 449 |
| TfiI | GAWTC | 5 cut(s) | 140, 578, 599, 642, 1292 |
| Tru1I | TTAA | 6 cut(s) | 99, 254, 351, 437, 1176, 1332 |
| Tru9I | TTAA | 6 cut(s) | 99, 254, 351, 437, 1176, 1332 |
| TseFI | GTSAC | 1 cut(s) | 1354 |
| TseI | GCWGC | 2 cut(s) | 104, 504 |
| Tsp45I | GTSAC | 1 cut(s) | 1354 |
| TspDTI | ATGAA | 9 cut(s) | 6, 40, 124, 336, 742, 1236, 1305, 1355, 1383 |
| TspGWI | ACGGA | 1 cut(s) | 1607 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1490 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 304 |
| Vha464I | CTTAAG | 1 cut(s) | 350 |
| VpaK11BI | GGWCC | 2 cut(s) | 265, 910 |
| XapI | RAATTY | 2 cut(s) | 801, 876 |
| XbaI | TCTAGA | 1 cut(s) | 1312 |
| XceI | RCATGY | 1 cut(s) | 1326 |
| XhoI | CTCGAG | 2 cut(s) | 199, 1388 |
| XmiI | GTMKAC | 2 cut(s) | 1447, 1494 |
| XmnI | GAANNNNTTC | 3 cut(s) | 391, 577, 789 |
| XspI | CTAG | 5 cut(s) | 681, 863, 995, 1088, 1313 |
| ZraI | GACGTC | 1 cut(s) | 130 |
| Zsp2I | ATGCAT | 2 cut(s) | 238, 1461 |