MD01G1191400.v1.1

RESISTANCE protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000067
Physical Location & Seq
Reverse (-)
28958068 .. 28963095
5028 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1191400.v1.1.491

Sequence Viewer

Length: 3948 bp
ATGGCTTATTCTCCGTCTTCTTCATCTGTTCTCGATTGGAAATATGATGTCTTCCTCAATTTTAGAGGCGAAGACACTCGGAAGACCTTCATCGGCCATCTGTACAAAGCTCTAGTTCACAAGTCAGTCAACACCTTCGTTGACGCTGAAGAGCTCAGAACAGGCAACGACCTTTCGCAACTACTGACAGCTATTAGCGAGTCAAGGCTTTCGATTGTAGTTTTTTCTCAAAACTACGCTTCTTCCACATGGTGCTTGAAAGAACTCGTCCAGATCCTGCATTGCATGGATCAGAAGCACCAGATTGTGATCCCCGTTTTCTATGAAGTAGATCCTTCTCATGTTCGTAAACTTGAGGGAAGTTTTGGGGAAGCTTTTTCTAAGCACGAACATGATCCCAACGCTGACATGGAAGAGGTTCAGCACTGGAGGTCCGCTCTACAAAGAGCTCCCAATTTATCAGGCTGGGATTCACGAAATTACCAGGATGATGCCAAGCTTATTGAGTTGATTGTAGATGATATTTTTGATAAATTGATCAACAACTCATCAAGTGAAAAGAACGGTTTGGTTGGAATGGATTCCCGCTTACAGAAAATGGATTCACTATTATGTCATGAGGTGGATGATGTTCGCTTTGTTGGAATATGGGGTATGGGTGGTATAGGCAAAACCACCATCGCCAGAGCTATGTATGACAAAATCAATCATCATTTCGAAGGTCGTTGCTTTCTTGAAAATGTGAAGGGACGTTTCCCGACGATTGATGCAGGTGAAGCACCATTAGATATGCAGGCGGAAATTCTGTCTAGCATCACGAATGCTAAGGTGGGGAGTTCAGAGATTTTGAGAAATGGTTTTCAGAAGATGGTGGAAAGAGTTGGTAAGAAAAAGATTTTACTTGTTCTTGATAATGTGGAGAATCCATTCCAAATTGAAGCTTTAATTGGACGACAACCTCCATTTGGTGGCGGAAGTCGAATCATTATAACAACTAGAGATAAACAGTCACTAAGTAGATTTGGTGATCAGATATATAAGCCAGAGTTGTTAAATGATGATGATGCTCTTGAACTGTTTATGCAGTATGCTTTCAGTACAAAGCAACGCACAGGAGAATACAGTGATATGTCAAGTCATTTCATAAAGTGTACTCAAGGTCTGCCTTTAGCACTTAAGGTCTTGGGAGCTTTTCTCGACAACAAAAGTGTACTTGTGTGGAAAGACGAGTTAGAGAAGATAGCGACAAACCCGCACCTGGGAATTCAGAAAGTCCTTCGAACAAGCTTTGATGGACTAGATCGTTTACAGAAGAAAATATTTCTTGATATTGCATGTTTCTTTAAACAAATGAAGAAAGACTATGCGATAAGGGTTATGGAGGGTTGTGGCTTCCATCCCCATACTGGACTGGACGTTCTAGTTGGTAGAGCTCTTGTAACTATCTCATCTGACGGTATAATCGAGATGCATGATTTACTAGAGGAAATGGGTTACGAGATCGTACGTCAGGAATCTATAGAAGAGCCTGGGAGGCGCAGTAGGTTGTGGAGCTATGAAGATGTTCGTCATGTGTTAACTCAAAATACGGCTTCAAAAGCAGTTGAAAGCATTATTGTGCATTGGCCGCACTCAAACGAAGTGTTACAATTAGATGCAGCTTTTGCTAAAATGACGGAACTAAGACTCCTTAGGGTCCACTCACACTATATACGAAAAAATGGAAGACATCAGTCGTTCGACTATGATCAAAGAGAATGTGAAGAATTAAAGTATCTTTCTGGAAAGTTAAGTTGTCTCTTCTGGCCCAATTGCCCCTTAAAATCTTTGTCGTCCAAGTTTAACGCGGAAAATCTTGTTGACCTTGACATGCAATATAGTCGTCTTCAACAGCTTTGGGAAGGAACTAAGTCTTTAGCAAAGTTGAAAGTTGTCAATTTAAAAGGCTCTCAACTTAAGGAAACTCCCGACTTCACTGGGGCGAAGAATCTTGAGAAACTAATTTTTGAGGGTTGCAGTTGTTTATCTGAGGTTCACCCATCCATTTCTGCCCTTGAAAAACTTGTTCTCTTGGATCTAAGTTGGTGCGTCAAACTCAAGGCCATTTCAAGCATCAATCATATGAAATCGCTTGAAACCTTGAATCTTTCTTTTTGCCGAGACATTGACAAATTCCCTGAGATTTCAGAAGTTATGGAGAAGCTTTCAGGACTTTATTTGCGAGGGACTGGCATAAAAGAACTGCCTCAATCAATTAACAATCTTACGGGGCTTCTTACTTTGAATCTTGAATATTGCAGAGAACTCAAGATTCTTCCAAACAGCATCGTTCAACTCAAGTCCCTTAAATTTCTTAATCTTTCTGGTTGTTCAAAGCTCGAGGTGTTTCCAGGAAATGTAGGAAATATGGAAGGATTAAAAGAGCTTTGCTTGGACGAGACATCTGTTAGAGAGCTTTGCCCATCAATTGCGTCTCTTCAAAACCTCGAGAGTTTGAGTTTAGAGCAGTGCAAGGAATTTCATAGTCTTCCAAGCAGCATTCATATGAGATCTCTTCGAACACTTAATCTTTCCGGCTGCTCAAATCTGGAGAAGTTTCCAGAAATTCCTGAAGTCATGGAGAACCTATCAGAGCTTAATTTAGAGGAGACTGCAATTACAGAGTTGCCCTTGTCCATTAAAAATTTAACGGGACTCGTTACTTTGAAACTGAAGGATTGCAGAAGATTAAGGATTCTTCCAAGCAGCATTCATATGAGATCTCTTCAAGCCCTAAATCTTTCTGGCTGCTCAAATCTGACGAAGGTTCCAGAGATTCTAGAAGTTATGAAGAACCTATTAGAGCTTCATTTAGATTGGACTACAATTGAAGACAATCATATGAGATCTGGCAGCCAAAATCTTGAGAACCTTCCTGTGTCTTCAGGACCTATGAAAGATCCATCAAGGCTCAGTTTAAATGGGATTGCAATCAAAGAATTGCCATCAGTAATTTATAATCTGAAGGGGCTTGTAACTTTAACGCTACGGTATAGCAAAGACTTTAAGAGTCTTACAAGCAGCATTTGTCAACTCACATCCCTTAGTTATCTCTCTCTTTCTGGTTGTACAGAGTTTGAGGTGTTTCCAGACATTTTTGAAGATATGGAACGATTAACCGGTCTTGATTTGGATGGAACATCTATTAGAGAGTTGCCCCCATCAATTGAACTCCTTCAAGGGCTTGTGACGTTAAATCTGAAAAACTGCAATAGCCTTGTCTCACTTCCGGACAGTATCTGCAATTTGTTAAGTCTTGAATGGCTGAATCTCAGTGGCTGCTCAAAACTTTCTAAGTTGCCTGAGAACTTGTGGTATTTGAAACGCCTTGACGTAAAGGGAGCTGGTATACGTAAAGACAACAATCATGGACCAACAGGCCGTTACCGCCCAAAACAGCGGTTTTTTAGCACTTTGGGGGATTTGGCTCAGTTTCGAAGCACTGCACCTTTGATTGATATTATGGGCTCAAACTGTAAGCAAAAAGAGTCGATGGCTGATGAGGAATCCCTTCTCACCTGGAGTGATGATTCAGAAATTTTGACTGATCCTGAGAAGTCATGCTATGCGGAAGAGACAAGGCAGATACATTTGGAGGTCCCTGAGCTAGAGAGCACGCTAGAGGGAACTGGTATACATCAAGACAACAATCACAGACCAACGGATTCCCACCCAAAACAGCATTTTTTCAGCACATTGGGGTATTTGGATCAGTTTCAGAGCACCACAACTTTGAGCGATATGGTGGACTCAAGCTACGAAGAAAAGGAGGTGGTGGCTGTTAAGGAAATTTTGAGTGATCTCAAGCAATCGAGACATGAGGAAGAGGAGGAGAGGGCGGTGGTAGCCTCCAGGAAAGGTGGGAGGATATGGGGTTGCATCTGTGGGTCACGGGGTAATGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_3 LRR_5 LRR_8 NB-ARC TIR

Protein Analysis

1316

Amino Acids

148.59

Weight (kDa)

5.7

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 14 - 192 3.3e-55 TIR domain
TIR_2 PF13676 17 - 112 4.2e-14 TIR domain
NB-ARC PF00931 203 - 368 4.9e-17 NB-ARC domain
WHD_ROQ1 PF23282 437 - 506 3.9e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 618 - 637 1.3e-06 Leucine Rich Repeat
LRR_13 PF23286 708 - 793 3.9e-15 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 738 - 814 2.3e-11 Leucine-rich repeat region
LRR_14 PF23598 818 - 971 2.9e-10 Leucine-rich repeat region
LRR_13 PF23286 850 - 934 9e-14 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 984 - 1056 2.2e-08 Leucine-rich repeat region
LRR_13 PF23286 1025 - 1111 4.3e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 1055 - 1128 2.5e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 989, 2997
AarI CACCTGC 1 cut(s) 761
Acc36I ACCTGC 1 cut(s) 761
AccB7I CCANNNNNTGG 1 cut(s) 968
AccBSI CCGCTC 1 cut(s) 437
AccI GTMKAC 2 cut(s) 3388, 3673
AccII CGCG 1 cut(s) 1847
AccIII TCCGGA 1 cut(s) 3268
AclWI GGATC 9 cut(s) 268, 297, 304, 326, 389, 2082, 2933, 3581, 3756
AcoI YGGCCR 2 cut(s) 94, 1625
AcsI RAATTY 9 cut(s) 801, 1263, 2171, 2348, 2516, 2604, 2683, 3576, 3828
AcuI CTGAAG 5 cut(s) 168, 2631, 2732, 2907, 3023
AdeI CACNNNGTG 1 cut(s) 252
AfaI GTAC 6 cut(s) 104, 1099, 1153, 1212, 1506, 3109
AfiI CCNNNNNNNGG 5 cut(s) 965, 968, 1258, 1259, 1406
AflII CTTAAG 2 cut(s) 1175, 1955
AgeI ACCGGT 1 cut(s) 3158
AjnI CCWGG 6 cut(s) 483, 1257, 1528, 2389, 3557, 3890
AjuI GAANNNNNNNTTGG 2 cut(s) 930, 962
AloI GAACNNNNNNTCC 4 cut(s) 1401, 1433, 1671, 1703
Alw21I GWGCWC 5 cut(s) 156, 451, 1435, 3656, 3764
Alw26I GTCTC 8 cut(s) 1802, 2154, 2432, 2478, 2642, 3265, 3608, 3847
AlwI GGATC 9 cut(s) 268, 297, 304, 326, 389, 2082, 2933, 3581, 3756
AlwNI CAGNNNCTG 3 cut(s) 277, 3279, 3318
Ama87I CYCGRG 2 cut(s) 2378, 2486
Aor13HI TCCGGA 1 cut(s) 3268
AoxI GGCC 5 cut(s) 94, 1625, 1805, 2100, 3418
ApeKI GCWGC 8 cut(s) 1658, 2535, 2577, 2745, 2787, 2892, 3060, 3318
ApoI RAATTY 9 cut(s) 801, 1263, 2171, 2348, 2516, 2604, 2683, 3576, 3828
AsiGI ACCGGT 1 cut(s) 3158
Asp700I GAANNNNTTC 7 cut(s) 86, 417, 580, 926, 1563, 3213, 3549
AspLEI GCGC 1 cut(s) 1539
AspS9I GGNCC 6 cut(s) 432, 1696, 1806, 2927, 3410, 3637
AsuHPI GGTGA 4 cut(s) 785, 1037, 2027, 3547
AsuII TTCGAA 4 cut(s) 717, 1279, 2557, 3475
AvaI CYCGRG 2 cut(s) 2378, 2486
AvaII GGWCC 5 cut(s) 432, 1696, 2927, 3410, 3637
AxyI CCTNAGG 1 cut(s) 1691
BanII GRGCYC 4 cut(s) 156, 451, 1435, 3509
BbsI GAAGAC 9 cut(s) 9, 43, 78, 89, 1732, 1877, 2519, 2877, 2913
Bbv12I GWGCWC 5 cut(s) 156, 451, 1435, 3656, 3764
BbvI GCAGC 8 cut(s) 1670, 2547, 2564, 2757, 2774, 2904, 3072, 3305
BceAI ACGGC 2 cut(s) 1605, 3405
BcgI CGANNNNNNTGC 2 cut(s) 1862, 1896
BciT130I CCWGG 6 cut(s) 485, 1259, 1530, 2391, 3559, 3892
BclI TGATCA 3 cut(s) 537, 1027, 1747
BcoDI GTCTC 8 cut(s) 1802, 2154, 2432, 2478, 2642, 3265, 3608, 3847
BfaI CTAG 9 cut(s) 113, 810, 996, 1298, 1421, 1481, 2819, 3647, 3659
BfmI CTRYAG 1 cut(s) 1518
BfrI CTTAAG 2 cut(s) 1175, 1955
BfuAI ACCTGC 1 cut(s) 761
BglI GCCNNNNNGGC 1 cut(s) 1534
BglII AGATCT 3 cut(s) 2549, 2759, 2885
BisI GCNGC 9 cut(s) 1628, 1659, 2536, 2578, 2746, 2788, 2893, 3061, 3319
BlsI GCNGC 9 cut(s) 1629, 1660, 2537, 2579, 2747, 2789, 2894, 3062, 3320
Bme1390I CCNGG 6 cut(s) 485, 1259, 1530, 2391, 3559, 3892
Bme18I GGWCC 5 cut(s) 432, 1696, 2927, 3410, 3637
BmeT110I CYCGRG 2 cut(s) 2378, 2486
BmgT120I GGNCC 6 cut(s) 432, 1696, 1806, 2927, 3410, 3637
BmiI GGNNCC 3 cut(s) 1697, 2808, 3639
BmrFI CCNGG 6 cut(s) 485, 1259, 1530, 2391, 3559, 3892
BmrI ACTGGG 1 cut(s) 1986
BmsI GCATC 9 cut(s) 481, 757, 822, 1054, 1458, 1645, 2121, 2334, 3927
BmuI ACTGGG 1 cut(s) 1986
BoxI GACNNNNGTC 1 cut(s) 1732
BpiI GAAGAC 9 cut(s) 9, 43, 78, 89, 1732, 1877, 2519, 2877, 2913
BplI GAGNNNNNCTC 4 cut(s) 421, 453, 1179, 1211
BpmI CTGGAG 4 cut(s) 448, 2609, 3580, 3874
Bpu10I CCTNAGC 2 cut(s) 825, 3642
Bpu14I TTCGAA 4 cut(s) 717, 1279, 2557, 3475
BpuEI CTTGAG 9 cut(s) 374, 1140, 2012, 2081, 2291, 2321, 2924, 3775, 3827
BsaAI YACGTR 1 cut(s) 3392
BsaBI GATNNNNATC 2 cut(s) 308, 2969
BsaJI CCNNGG 2 cut(s) 1258, 1529
BsaWI WCCGGW 2 cut(s) 3158, 3268
BsaXI ACNNNNNCTCC 4 cut(s) 1525, 1555, 1671, 1701
Bsc4I CCNNNNNNNGG 5 cut(s) 965, 968, 1258, 1259, 1406
Bse118I RCCGGY 1 cut(s) 3158
Bse1I ACTGG 6 cut(s) 431, 1411, 1416, 1981, 2233, 3673
Bse21I CCTNAGG 1 cut(s) 1691
Bse3DI GCAATG 1 cut(s) 280
Bse8I GATNNNNATC 2 cut(s) 308, 2969
BseAI TCCGGA 1 cut(s) 3268
BseBI CCWGG 6 cut(s) 485, 1259, 1530, 2391, 3559, 3892
BseDI CCNNGG 2 cut(s) 1258, 1529
BseGI GGATG 6 cut(s) 493, 631, 1396, 2039, 3077, 3178
BseJI GATNNNNATC 2 cut(s) 308, 2969
BseLI CCNNNNNNNGG 5 cut(s) 965, 968, 1258, 1259, 1406
BseMI GCAATG 1 cut(s) 280
BseMII CTCAG 9 cut(s) 169, 2019, 2169, 2965, 3325, 3333, 3482, 3582, 3633
BseNI ACTGG 6 cut(s) 431, 1411, 1416, 1981, 2233, 3673
BseRI GAGGAG 3 cut(s) 2660, 3881, 3884
BseXI GCAGC 8 cut(s) 1670, 2547, 2564, 2757, 2774, 2904, 3072, 3305
BseYI CCCAGC 1 cut(s) 465
BsgI GTGCAG 1 cut(s) 3468
Bsh1236I CGCG 1 cut(s) 1847
BshFI GGCC 5 cut(s) 96, 1627, 1807, 2102, 3420
BshTI ACCGGT 1 cut(s) 3158
BsiHKAI GWGCWC 5 cut(s) 156, 451, 1435, 3656, 3764
BsiHKCI CYCGRG 2 cut(s) 2378, 2486
BsiSI CCGG 3 cut(s) 2574, 3159, 3269
BsiWI CGTACG 1 cut(s) 1504
BslFI GGGAC 5 cut(s) 762, 2239, 2326, 2706, 3623
BslI CCNNNNNNNGG 5 cut(s) 965, 968, 1258, 1259, 1406
BsmAI GTCTC 8 cut(s) 1802, 2154, 2432, 2478, 2642, 3265, 3608, 3847
BsmBI CGTCTC 1 cut(s) 2478
BsmFI GGGAC 5 cut(s) 762, 2239, 2326, 2706, 3623
BsmI GAATGC 3 cut(s) 826, 2538, 2748
BsnI GGCC 5 cut(s) 96, 1627, 1807, 2102, 3420
BsoBI CYCGRG 2 cut(s) 2378, 2486
Bsp119I TTCGAA 4 cut(s) 717, 1279, 2557, 3475
Bsp1286I GDGCHC 6 cut(s) 156, 451, 1435, 3509, 3656, 3764
Bsp13I TCCGGA 1 cut(s) 3268
Bsp1407I TGTACA 2 cut(s) 102, 3107
BspANI GGCC 5 cut(s) 96, 1627, 1807, 2102, 3420
BspCNI CTCAG 9 cut(s) 168, 2020, 2170, 2964, 3324, 3334, 3481, 3583, 3634
BspEI TCCGGA 1 cut(s) 3268
BspFNI CGCG 1 cut(s) 1847
BspHI TCATGA 1 cut(s) 616
BspLI GGNNCC 3 cut(s) 1697, 2808, 3639
BspMI ACCTGC 1 cut(s) 761
BspPI GGATC 9 cut(s) 268, 297, 304, 326, 389, 2082, 2933, 3581, 3756
BspQI GCTCTTC 2 cut(s) 144, 1518
BspT104I TTCGAA 4 cut(s) 717, 1279, 2557, 3475
BspTI CTTAAG 2 cut(s) 1175, 1955
BsrBI CCGCTC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 280
BsrFI RCCGGY 1 cut(s) 3158
BsrGI TGTACA 2 cut(s) 102, 3107
BsrI ACTGG 6 cut(s) 431, 1411, 1416, 1981, 2233, 3673
BssAI RCCGGY 1 cut(s) 3158
BssECI CCNNGG 2 cut(s) 1258, 1529
BssNAI GTATAC 2 cut(s) 3389, 3674
Bst1107I GTATAC 2 cut(s) 3389, 3674
Bst2UI CCWGG 6 cut(s) 485, 1259, 1530, 2391, 3559, 3892
Bst4CI ACNGT 8 cut(s) 566, 1008, 1077, 1124, 1457, 3030, 3275, 3515
Bst6I CTCTTC 9 cut(s) 144, 408, 1518, 1805, 2481, 2559, 2769, 3606, 3858
BstAFI CTTAAG 2 cut(s) 1175, 1955
BstAPI GCANNNNNTGC 1 cut(s) 1664
BstAUI TGTACA 2 cut(s) 102, 3107
BstBAI YACGTR 1 cut(s) 3392
BstBI TTCGAA 4 cut(s) 717, 1279, 2557, 3475
BstC8I GCNNGC 2 cut(s) 795, 3656
BstF5I GGATG 6 cut(s) 493, 631, 1396, 2039, 3077, 3178
BstFNI CGCG 1 cut(s) 1847
BstHHI GCGC 1 cut(s) 1539
BstMAI GTCTC 8 cut(s) 1802, 2154, 2432, 2478, 2642, 3265, 3608, 3847
BstMWI GCNNNNNNNGC 7 cut(s) 776, 1534, 1598, 1627, 1664, 3426, 3884
BstNI CCWGG 6 cut(s) 485, 1259, 1530, 2391, 3559, 3892
BstNSI RCATGY 2 cut(s) 1338, 1873
BstPAI GACNNNNGTC 1 cut(s) 1732
BstSCI CCNGG 6 cut(s) 483, 1257, 1528, 2389, 3557, 3890
BstSFI CTRYAG 1 cut(s) 1518
BstSNI TACGTA 1 cut(s) 3392
BstUI CGCG 1 cut(s) 1847
BstV1I GCAGC 8 cut(s) 1670, 2547, 2564, 2757, 2774, 2904, 3072, 3305
BstV2I GAAGAC 9 cut(s) 9, 43, 78, 89, 1732, 1877, 2519, 2877, 2913
BstX2I RGATCY 7 cut(s) 273, 331, 2074, 2549, 2759, 2885, 2938
BstYI RGATCY 7 cut(s) 273, 331, 2074, 2549, 2759, 2885, 2938
BstZ17I GTATAC 2 cut(s) 3389, 3674
Bsu36I CCTNAGG 1 cut(s) 1691
BsuRI GGCC 5 cut(s) 96, 1627, 1807, 2102, 3420
BtgZI GCGATG 1 cut(s) 664
BtsCI GGATG 6 cut(s) 493, 631, 1396, 2039, 3077, 3178
BtsI GCAGTG 2 cut(s) 2513, 3480
BtsIMutI CAGTG 6 cut(s) 424, 1129, 1974, 2513, 3319, 3480
BveI ACCTGC 1 cut(s) 761
Cac8I GCNNGC 2 cut(s) 795, 3656
CaiI CAGNNNCTG 3 cut(s) 277, 3279, 3318
CciI TCATGA 1 cut(s) 616
CfoI GCGC 1 cut(s) 1539
Cfr10I RCCGGY 1 cut(s) 3158
Cfr13I GGNCC 6 cut(s) 432, 1696, 1806, 2927, 3410, 3637
CseI GACGC 3 cut(s) 152, 2077, 2460
Csp6I GTAC 6 cut(s) 103, 1098, 1152, 1211, 1505, 3108
CspAI ACCGGT 1 cut(s) 3158
CviQI GTAC 6 cut(s) 103, 1098, 1152, 1211, 1505, 3108
DraI TTTAAA 3 cut(s) 1345, 1941, 2958
DraIII CACNNNGTG 1 cut(s) 252
EaeI YGGCCR 2 cut(s) 94, 1625
Eam1104I CTCTTC 9 cut(s) 144, 408, 1518, 1805, 2481, 2559, 2769, 3606, 3858
EarI CTCTTC 9 cut(s) 144, 408, 1518, 1805, 2481, 2559, 2769, 3606, 3858
EciI GGCGGA 2 cut(s) 812, 987
Ecl136II GAGCTC 3 cut(s) 154, 449, 1433
Eco105I TACGTA 1 cut(s) 3392
Eco24I GRGCYC 4 cut(s) 156, 451, 1435, 3509
Eco47I GGWCC 5 cut(s) 432, 1696, 2927, 3410, 3637
Eco53kI GAGCTC 3 cut(s) 154, 449, 1433
Eco57I CTGAAG 5 cut(s) 168, 2631, 2732, 2907, 3023
Eco81I CCTNAGG 1 cut(s) 1691
Eco88I CYCGRG 2 cut(s) 2378, 2486
EcoICRI GAGCTC 3 cut(s) 154, 449, 1433
EcoO109I RGGNCCY 2 cut(s) 2927, 3637
EcoRI GAATTC 1 cut(s) 1263
EcoRII CCWGG 6 cut(s) 483, 1257, 1528, 2389, 3557, 3890
EcoT22I ATGCAT 1 cut(s) 1473
EcoT38I GRGCYC 4 cut(s) 156, 451, 1435, 3509
Esp3I CGTCTC 1 cut(s) 2478
FaqI GGGAC 5 cut(s) 762, 2239, 2326, 2706, 3623
FauI CCCGC 2 cut(s) 593, 1260
FauNDI CATATG 4 cut(s) 2121, 2544, 2754, 2880
FbaI TGATCA 3 cut(s) 537, 1027, 1747
FblI GTMKAC 2 cut(s) 3388, 3673
Fnu4HI GCNGC 9 cut(s) 1628, 1659, 2536, 2578, 2746, 2788, 2893, 3061, 3319
FokI GGATG 6 cut(s) 500, 638, 1383, 2026, 3064, 3185
FriOI GRGCYC 4 cut(s) 156, 451, 1435, 3509
Fsp4HI GCNGC 9 cut(s) 1628, 1659, 2536, 2578, 2746, 2788, 2893, 3061, 3319
FspBI CTAG 9 cut(s) 113, 810, 996, 1298, 1421, 1481, 2819, 3647, 3659
GlaI GCGC 1 cut(s) 1538
GluI GCNGC 9 cut(s) 1628, 1659, 2536, 2578, 2746, 2788, 2893, 3061, 3319
GsaI CCCAGC 1 cut(s) 469
GsuI CTGGAG 4 cut(s) 448, 2609, 3580, 3874
HaeIII GGCC 5 cut(s) 96, 1627, 1807, 2102, 3420
HapII CCGG 3 cut(s) 2574, 3159, 3269
HgaI GACGC 3 cut(s) 152, 2077, 2460
HhaI GCGC 1 cut(s) 1539
Hin6I GCGC 1 cut(s) 1537
HinP1I GCGC 1 cut(s) 1537
HincII GTYRAC 5 cut(s) 130, 142, 1578, 1861, 3071
HindII GTYRAC 5 cut(s) 130, 142, 1578, 1861, 3071
HindIII AAGCTT 5 cut(s) 372, 497, 939, 1285, 2201
HpaI GTTAAC 1 cut(s) 1578
HpaII CCGG 3 cut(s) 2574, 3159, 3269
HphI GGTGA 4 cut(s) 785, 1037, 2027, 3547
Hpy99I CGWCG 1 cut(s) 763
HpyCH4III ACNGT 8 cut(s) 566, 1008, 1077, 1124, 1457, 3030, 3275, 3515
HpyCH4IV ACGT 6 cut(s) 751, 1416, 1507, 3230, 3372, 3391
HpyF10VI GCNNNNNNNGC 7 cut(s) 776, 1534, 1598, 1627, 1664, 3426, 3884
HpySE526I ACGT 6 cut(s) 751, 1416, 1507, 3230, 3372, 3391
HspAI GCGC 1 cut(s) 1537
Kpn2I TCCGGA 1 cut(s) 3268
Ksp22I TGATCA 3 cut(s) 537, 1027, 1747
KspAI GTTAAC 1 cut(s) 1578
LguI GCTCTTC 2 cut(s) 144, 1518
LmnI GCTCC 4 cut(s) 454, 1187, 1551, 3380
Lsp1109I GCAGC 8 cut(s) 1670, 2547, 2564, 2757, 2774, 2904, 3072, 3305
LweI GCATC 9 cut(s) 481, 757, 822, 1054, 1458, 1645, 2121, 2334, 3927
MaeI CTAG 9 cut(s) 113, 810, 996, 1298, 1421, 1481, 2819, 3647, 3659
MaeII ACGT 6 cut(s) 751, 1416, 1507, 3230, 3372, 3391
MaeIII GTNAC 9 cut(s) 1008, 1438, 1493, 1644, 2698, 3013, 3226, 3422, 3927
MbiI CCGCTC 1 cut(s) 437
MfeI CAATTG 4 cut(s) 1810, 2466, 2865, 3204
MflI RGATCY 7 cut(s) 273, 331, 2074, 2549, 2759, 2885, 2938
MhlI GDGCHC 6 cut(s) 156, 451, 1435, 3509, 3656, 3764
MlyI GAGTC 6 cut(s) 209, 1680, 2688, 3058, 3536, 3782
MmeI TCCRAC 2 cut(s) 553, 622
Mph1103I ATGCAT 1 cut(s) 1473
MroI TCCGGA 1 cut(s) 3268
MroXI GAANNNNTTC 7 cut(s) 86, 417, 580, 926, 1563, 3213, 3549
MslI CAYNNNNRTG 5 cut(s) 390, 610, 1616, 2543, 2753
MspA1I CMGCKG 1 cut(s) 3439
MspCI CTTAAG 2 cut(s) 1175, 1955
MspI CCGG 3 cut(s) 2574, 3159, 3269
MspR9I CCNGG 6 cut(s) 485, 1259, 1530, 2391, 3559, 3892
MunI CAATTG 4 cut(s) 1810, 2466, 2865, 3204
Mva1269I GAATGC 3 cut(s) 826, 2538, 2748
MvaI CCWGG 6 cut(s) 485, 1259, 1530, 2391, 3559, 3892
MvnI CGCG 1 cut(s) 1847
MwoI GCNNNNNNNGC 7 cut(s) 776, 1534, 1598, 1627, 1664, 3426, 3884
NdeI CATATG 4 cut(s) 2121, 2544, 2754, 2880
NlaIV GGNNCC 3 cut(s) 1697, 2808, 3639
NmeAIII GCCGAG 1 cut(s) 2183
NmuCI GTSAC 3 cut(s) 1008, 3226, 3927
NsiI ATGCAT 1 cut(s) 1473
NspI RCATGY 2 cut(s) 1338, 1873
NspV TTCGAA 4 cut(s) 717, 1279, 2557, 3475
PaeR7I CTCGAG 2 cut(s) 2378, 2486
PagI TCATGA 1 cut(s) 616
PaqCI CACCTGC 1 cut(s) 761
PciSI GCTCTTC 2 cut(s) 144, 1518
PcsI WCGNNNNNNNCGW 1 cut(s) 1461
PctI GAATGC 3 cut(s) 826, 2538, 2748
PdmI GAANNNNTTC 7 cut(s) 86, 417, 580, 926, 1563, 3213, 3549
Pfl23II CGTACG 1 cut(s) 1504
PflMI CCANNNNNTGG 1 cut(s) 968
PfoI TCCNGGA 2 cut(s) 2389, 3890
PinAI ACCGGT 1 cut(s) 3158
PkrI GCNGC 9 cut(s) 1629, 1660, 2537, 2579, 2747, 2789, 2894, 3062, 3320
PleI GAGTC 6 cut(s) 208, 1680, 2688, 3057, 3535, 3782
PpsI GAGTC 6 cut(s) 208, 1680, 2688, 3057, 3535, 3782
Ppu21I YACGTR 1 cut(s) 3392
PpuMI RGGWCCY 2 cut(s) 2927, 3637
PshAI GACNNNNGTC 1 cut(s) 1732
PsiI TTATAA 2 cut(s) 989, 2997
Psp124BI GAGCTC 3 cut(s) 156, 451, 1435
Psp5II RGGWCCY 2 cut(s) 2927, 3637
Psp6I CCWGG 6 cut(s) 483, 1257, 1528, 2389, 3557, 3890
PspFI CCCAGC 1 cut(s) 465
PspGI CCWGG 6 cut(s) 483, 1257, 1528, 2389, 3557, 3890
PspLI CGTACG 1 cut(s) 1504
PspN4I GGNNCC 3 cut(s) 1697, 2808, 3639
PspPI GGNCC 6 cut(s) 432, 1696, 1806, 2927, 3410, 3637
PspPPI RGGWCCY 2 cut(s) 2927, 3637
PspXI VCTCGAGB 1 cut(s) 2378
PstNI CAGNNNCTG 3 cut(s) 277, 3279, 3318
PsuI RGATCY 7 cut(s) 273, 331, 2074, 2549, 2759, 2885, 2938
RsaI GTAC 6 cut(s) 104, 1099, 1153, 1212, 1506, 3109
RsaNI GTAC 6 cut(s) 103, 1098, 1152, 1211, 1505, 3108
RseI CAYNNNNRTG 5 cut(s) 390, 610, 1616, 2543, 2753
SacI GAGCTC 3 cut(s) 156, 451, 1435
SapI GCTCTTC 2 cut(s) 144, 1518
SatI GCNGC 9 cut(s) 1628, 1659, 2536, 2578, 2746, 2788, 2893, 3061, 3319
Sau96I GGNCC 6 cut(s) 432, 1696, 1806, 2927, 3410, 3637
SchI GAGTC 6 cut(s) 209, 1680, 2688, 3058, 3536, 3782
ScrFI CCNGG 6 cut(s) 485, 1259, 1530, 2391, 3559, 3892
SduI GDGCHC 6 cut(s) 156, 451, 1435, 3509, 3656, 3764
SfaNI GCATC 9 cut(s) 481, 757, 822, 1054, 1458, 1645, 2121, 2334, 3927
SfcI CTRYAG 1 cut(s) 1518
Sfr274I CTCGAG 2 cut(s) 2378, 2486
SfuI TTCGAA 4 cut(s) 717, 1279, 2557, 3475
SinI GGWCC 5 cut(s) 432, 1696, 2927, 3410, 3637
SlaI CTCGAG 2 cut(s) 2378, 2486
SmiMI CAYNNNNRTG 5 cut(s) 390, 610, 1616, 2543, 2753
SnaBI TACGTA 1 cut(s) 3392
SspI AATATT 2 cut(s) 1320, 2294
SspMI CTAG 9 cut(s) 113, 810, 996, 1298, 1421, 1481, 2819, 3647, 3659
SstI GAGCTC 3 cut(s) 156, 451, 1435
StyD4I CCNGG 6 cut(s) 483, 1257, 1528, 2389, 3557, 3890
TaaI ACNGT 8 cut(s) 566, 1008, 1077, 1124, 1457, 3030, 3275, 3515
TaiI ACGT 6 cut(s) 754, 1419, 1510, 3233, 3375, 3394
TatI WGTACW 5 cut(s) 102, 1097, 1151, 1210, 3107
TauI GCSGC 1 cut(s) 1630
TscAI CASTG 6 cut(s) 431, 1129, 1981, 2513, 3319, 3487
TseFI GTSAC 3 cut(s) 1008, 3226, 3927
TseI GCWGC 8 cut(s) 1658, 2535, 2577, 2745, 2787, 2892, 3060, 3318
Tsp45I GTSAC 3 cut(s) 1008, 3226, 3927
TspGWI ACGGA 2 cut(s) 1691, 3716
TspRI CASTG 6 cut(s) 431, 1129, 1981, 2513, 3319, 3487
Van91I CCANNNNNTGG 1 cut(s) 968
Vha464I CTTAAG 2 cut(s) 1175, 1955
VpaK11BI GGWCC 5 cut(s) 432, 1696, 2927, 3410, 3637
XapI RAATTY 9 cut(s) 801, 1263, 2171, 2348, 2516, 2604, 2683, 3576, 3828
XbaI TCTAGA 1 cut(s) 2818
XceI RCATGY 2 cut(s) 1338, 1873
XcmI CCANNNNNNNNNTGG 2 cut(s) 406, 1403
XhoI CTCGAG 2 cut(s) 2378, 2486
XmiI GTMKAC 2 cut(s) 3388, 3673
XmnI GAANNNNTTC 7 cut(s) 86, 417, 580, 926, 1563, 3213, 3549
XspI CTAG 9 cut(s) 113, 810, 996, 1298, 1421, 1481, 2819, 3647, 3659
Zsp2I ATGCAT 1 cut(s) 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.