Rw0G000410

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00013
OG0000067
Physical Location & Seq
Reverse (-)
13329 .. 18056
4728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G000410.1

Sequence Viewer

Length: 3789 bp
ATGGCTTCATCTTCATCTGGTGGTCGTTGCAATTATGATGTCTTCATCAGTTTTAGAGGGGAAGACACCCGAAAGATCTTCGTCGGTCATCTTTACAGAGCTCTGCAACAGAAAGCAATCAACACCTTCATCGATTCTGAAGGGCTCAGTAAAGGCAACAAGATTTCCGAGCTGCTGGAAGCTATTGGTAACTCAAGGCTTTCCATTGTAGTTTTATCTCAAAACTATGCCTCTTCCACATGGTGCTTAAAAGAGCTTGCCAAAATCTTGGATTGCATGGTAACAGAGAAGCAGATAGTGGTGCCTATCTTCTACGAAGTAGATCCCTCTGATCTACGGAAAATCAATGGAAAATTTGCTGAAGCTTTTGCCAGACATGGAGAAAATTCTAACCTCGACAAGGAAGAGTTGGAGTGCTGGAGGTCCGCTCTAATTAGAGTTGCGGATCTATCTGGCTGGGATTCCACCAAATATGAGGATGATGGCAGGCTTATTGAGGAAATTGTTGAAAATACTTTTAAAAAACTGATCCACACTTCGTCCAATAAAGCCAATGGCTTGGTTGGAATGGATTCCCACATTACAGAAATGAATTCAAGATTATGTCTACAGGTGAATGATGTTCGTGTTGTTGGATTATGGGGTATGGGCGGTATAGGAAAAACAACCATTGCTAGAGCTGTGTTTGACGAGATTGCTTGGCAATTTGAACACTCCTGCTTTGTTGATAATGTCAAAGAAGCATTTATAAACAAGCGTGAAGTACAAATGCAGGAAGAACTTATATCTATATTGCTGAAGGAAAAGGTGCGGAGTCTGGGTTTGAACAGAGGTTGGAATATGATAAAGGAAAGGCTAGGTATGGAAAAAGTCTTTGTTGTTCTTGATGATGTGGAAACTTTTTCCCAGATTGAAGCCTTGCTTGGAAAACCACATGCATTTGGTGAGGGAAGTAGAGTCATTGTAACAACTAGAAATAAGCAATCACTAAGTGGAGTTAATGAGACATATAAGCCCGCGTATTTAAGTAATGATGAAGCTCTTGAGCTATTTGTGAAATATGCCTTCAGAACAAATCAACCCAACGGAGACTACGATCATCTCTCAAGGTGTGCCATGGAATATGCTCAAGGTTTGCCCTTAGCGCTTAAAGTCTTGGGGGCTTTTCTTGATAACAAAAGTGTGTGTGAATGGGAAGCCGTGTTGATGAAATTGAAGAAAACCCCACAAATAGAGCTTCAGGATGTGCTTAGAACAAGCTTTGATGAACTAGACGATTCAGAGAAGGACATATTTCTAGATATTGCATGTCTCTTTAGAGGATGGACCAAAAGTTCTGTCACAGAATTATTAGACAGTTTTGGCTTCTTTCCCCACAATGGATTGAGAGTTCTAATTGACAGAGCTCTCATAACTATATCACGTTTCGATCAAGTCGACATGCATGATTTAATACAAGAAATGGGTAGAGAAATCGTCCGCCTAGAATCTACCAAAGAACCTGGGAAGCGCAGTAGGTTGTGGAGTAGTGAAGATGCTAATTGTGTATTGACTCAAAAAACGGCTACTGATGCAGTCGAATGCTTAGTGCTAAAGTTGTCGAACTCAAAAGTTGACCTATGCATAGATACTGAGGCCTTTGTAAGAATGACGAAGCTAAGACTACTCATAATCTATTATTCAATCCCTGCAGCGTTCAATTTTGCCTACTGCTGGCCACACAAGAGCATATGCCCAGTTGGTGGCCGTAAACAGCTCACGAGGGGAGACTTCGAGTTTCTTTCTCATGAGTTGAGGTGTCTCTTATGGCATGGATGCCCACTAAAGTCTTTGCCATCAACCTATATCCCAAAGAATCTTGTTAAACTTGATATGCGTGGTAGTCACATTCAAGAACTTTGGGAAGGAATCAAGCCCCTACAAAATCTGAAAGTCATCACGTTAAGTCACTCTCAATACCTTGTCAAAATCCCTGACTTGACAGAGGCGGTAAATCTCGAGAAACTACTTCTTAATGATTGTTCAAGTTTACTTGAGGTCCATCCATCCATTTCTGCTCTTCAAAACCTTGATTTCTTGAATTTAAAGCGGTGCAAAGGACTCAACAATCTCCCAAGCAGCATTCATATGAAATCTCTAAAAACCCTTGATATTATTGGCTGTTCAAATCTTCAAAAGTTTCCAGAAATTTCTCAAGTTATGAATAAGTTAACAGATCTTTACTTAGATGGGACTGCAATTAAAGAACTGCCCTCATCAATTAGTAATCTTACAGGCCTTGTTACTTTGGACCTCAGTGGTTGCAGAGAATTTAAGAGTTTGCCTGGCAGCATTCATATGAAATCTCTGAAAACGCTTAATCTTTCTGGCTGTTCAAATGTGGATAAGTTTCCAGAGATTCCAGAGGTTATGACGGAGCTCAAGGAGGTTTATCTAGATGAGACTGCAATTAAAGAACTACCCTCATCAATTGAACGTCTTATGGGGATTAAAATTTTAAGTATGCGGAACTGCAAAAGCATTGAATTTCTTCCCGACAGTATCTGTAATTTGGCGGACCTTACACATCTCAATCTGTCTGGGTGCTCAGTACTTCATAACTTGCCTGAGAAATTGGGAGACTTAGAATCCTTACAGGTCCTTGACGTAAGTTATAGTGGTATAAACCGTCTTCCATTTCCTATCCTTTATGTGGGCGACACATTATCATGTATTGGATGTAAAGAGATGACGGCACCCTTGTCAGCATTGCCATCATCAATGGAAGAATTCTGGAGTTACTCTGTTCTGCTACATCTTGATTTAAGTGACTGCAGTCTGTCGGAGTTATCAGATGGTATTGCTGATTTGTCTTCACTGAAAACATTAAAGCTCTGCAGAAACAATTTGGAGAGCTTACCTGTGACAATTAATCGACTTGGTTGTTTAACGCATCTTGAATTGGAAGCTTGCAAGAGGCTGAAATCAATACCAGAGCTTTCATCGAGTATAAATTACATAGATGCACATGATTGCATAGCTTTGGAAACAGTTTCAAAGCCAAAGCCTCAGTACCAGACGGATCATTTCTTTACATTTTCTAATTGCCTCCAGCTGGTACAAACAAATCTATTTACAGATATTGTGGAAAGACATTCACATTTGCAGGGTAGTCGTAGTTACCTGGGACCTCTTCAGTTAAATATGTCTCTTCCTGGGAGTGAAGTTCCTGATTGGTTCACTCATCAGAGTAGGGGAGTTTCAGTGAGTGCGCAGCTCCCACCAAATTGGTATGACAGTAAGTTTTTGGGATTCGCTATCTGCGCTGTTCTTAAATGCCAGGGAATTAATTATCATACAGATACTTTATCTGCTGCTTGTTTATGTATCTTCAATGGAAATAGTGGCGAATGTAGTTTCAGTTTTGATATGCTCGACTCAGTGTTCAGAATTAATAGAAACTTTAGGTCAGATCACATGTTCCTGGGATATGTGTCGTGGTCAGATCACAAGGTCCTGATTGAAGAAGGAAAGCTGGCCCATGAAGCCTACAATGAGGCCACATTTCAGATTGTATTAAGTGAATTTGTAAATACAAAACTCCGCGTCAGAAGCTGCGGAGTTCGTTTGGTATATGCTAATCCAGATGAAATGCTGAATCTAAACATGCCACAGCCCCAAGTTCATGTGGACAAAAACAAAAACAGAAAGAGGAGGAGACAGAGTATCTGGCGATGGGGAGATATTGGATTTGGGCAGCCACGATGGTGCCAAATTCACAAAATGATGGCAAAGAAGAGAAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1262

Amino Acids

142.58

Weight (kDa)

6.28

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 188 7.1e-52 TIR domain
TIR_2 PF13676 14 - 115 1.7e-15 TIR domain
NB-ARC PF00931 194 - 359 9.7e-18 NB-ARC domain
WHD_ROQ1 PF23282 427 - 496 1.5e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 702 - 809 6.4e-08 Leucine-rich repeat region
LRR_13 PF23286 710 - 780 2.1e-14 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 780 - 866 4.3e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 782 - 862 7.2e-07 Leucine-rich repeat region
LRR_14 PF23598 848 - 919 7e-07 Leucine-rich repeat region
LRR_14 PF23598 920 - 993 8.5e-08 Leucine-rich repeat region
LRR_8 PF13855 932 - 984 6e-06 Leucine rich repeat
C-JID PF20160 1064 - 1208 2.4e-20 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 749
Acc16I TGCGCA 1 cut(s) 3252
AccB1I GGYRCC 3 cut(s) 301, 2734, 3747
AccB7I CCANNNNNTGG 1 cut(s) 1742
AccBSI CCGCTC 1 cut(s) 428
AccI GTMKAC 2 cut(s) 607, 1437
AccII CGCG 2 cut(s) 1019, 3585
AclWI GGATC 4 cut(s) 317, 453, 523, 3069
AcoI YGGCCR 2 cut(s) 1715, 1744
AcuI CTGAAG 6 cut(s) 159, 381, 818, 1051, 1223, 3158
AdeI CACNNNGTG 2 cut(s) 243, 992
AfaI GTAC 4 cut(s) 765, 2592, 3053, 3099
AfeI AGCGCT 1 cut(s) 1146
AfiI CCNNNNNNNGG 6 cut(s) 400, 1379, 1713, 1742, 2692, 3094
AflIII ACRYGT 1 cut(s) 3456
AjnI CCWGG 6 cut(s) 1501, 2323, 3162, 3193, 3318, 3462
AjuI GAANNNNNNNTTGG 2 cut(s) 906, 938
AleI CACNNNNGTG 1 cut(s) 3745
AloI GAACNNNNNNTCC 4 cut(s) 1318, 1350, 1374, 1406
Alw21I GWGCWC 4 cut(s) 103, 1408, 2421, 2588
Alw26I GTCTC 9 cut(s) 998, 1083, 1316, 1761, 1805, 2435, 2613, 3192, 3691
AlwI GGATC 4 cut(s) 317, 453, 523, 3069
AlwNI CAGNNNCTG 2 cut(s) 2544, 3594
Ama87I CYCGRG 1 cut(s) 1997
Aor51HI AGCGCT 1 cut(s) 1146
AoxI GGCC 6 cut(s) 1635, 1715, 1744, 2275, 3516, 3537
ApeKI GCWGC 8 cut(s) 172, 1691, 2118, 2328, 3253, 3353, 3594, 3736
ArsI GACNNNNNNTTYG 2 cut(s) 1323, 1355
AseI ATTAAT 3 cut(s) 2910, 3327, 3432
Asp700I GAANNNNTTC 2 cut(s) 571, 2529
AspLEI GCGC 4 cut(s) 1147, 1512, 3253, 3305
AspS9I GGNCC 9 cut(s) 423, 1326, 2038, 2290, 2557, 2638, 3167, 3493, 3517
AsuHPI GGTGA 2 cut(s) 625, 956
AvaI CYCGRG 1 cut(s) 1997
AvaII GGWCC 8 cut(s) 423, 1326, 2038, 2290, 2557, 2638, 3167, 3493
BalI TGGCCA 1 cut(s) 1717
BanI GGYRCC 3 cut(s) 301, 2734, 3747
BanII GRGCYC 4 cut(s) 103, 147, 1408, 2421
BauI CACGAG 1 cut(s) 1759
BbsI GAAGAC 4 cut(s) 34, 69, 2663, 2844
Bbv12I GWGCWC 4 cut(s) 103, 1408, 2421, 2588
BbvI GCAGC 8 cut(s) 159, 1703, 2130, 2340, 3265, 3340, 3581, 3748
BceAI ACGGC 4 cut(s) 1184, 1578, 1731, 2748
BcgI CGANNNNNNTGC 2 cut(s) 3134, 3168
BciT130I CCWGG 6 cut(s) 1503, 2325, 3164, 3195, 3320, 3464
BcoDI GTCTC 9 cut(s) 998, 1083, 1316, 1761, 1805, 2435, 2613, 3192, 3691
BfaI CTAG 7 cut(s) 675, 857, 972, 1271, 1298, 1484, 2435
BfmI CTRYAG 4 cut(s) 608, 1689, 2812, 2875
BfoI RGCGCY 1 cut(s) 1148
BglII AGATCT 2 cut(s) 75, 2215
BisI GCNGC 8 cut(s) 173, 1692, 2119, 2329, 3254, 3354, 3595, 3737
BlsI GCNGC 8 cut(s) 174, 1693, 2120, 2330, 3255, 3355, 3596, 3738
BmcAI AGTACT 1 cut(s) 2592
Bme1390I CCNGG 6 cut(s) 1503, 2325, 3164, 3195, 3320, 3464
Bme18I GGWCC 8 cut(s) 423, 1326, 2038, 2290, 2557, 2638, 3167, 3493
BmeT110I CYCGRG 1 cut(s) 1997
BmgT120I GGNCC 9 cut(s) 423, 1326, 2038, 2290, 2557, 2638, 3167, 3493, 3517
BmiI GGNNCC 4 cut(s) 303, 2736, 3168, 3749
BmrFI CCNGG 6 cut(s) 1503, 2325, 3164, 3195, 3320, 3464
BmrI ACTGGG 1 cut(s) 1730
BmsI GCATC 5 cut(s) 1525, 1561, 1805, 2941, 2992
BmuI ACTGGG 1 cut(s) 1730
BoxI GACNNNNGTC 1 cut(s) 2814
BpiI GAAGAC 4 cut(s) 34, 69, 2663, 2844
BplI GAGNNNNNCTC 2 cut(s) 412, 444
BpmI CTGGAG 3 cut(s) 439, 2794, 3074
Bpu10I CCTNAGC 1 cut(s) 1141
BpuEI CTTGAG 7 cut(s) 178, 1064, 1090, 1113, 2054, 2178, 2405
Bsa29I ATCGAT 1 cut(s) 132
BsaJI CCNNGG 6 cut(s) 1116, 1502, 3163, 3194, 3319, 3463
BsaXI ACNNNNNCTCC 6 cut(s) 805, 835, 3228, 3258, 3688, 3718
Bsc4I CCNNNNNNNGG 6 cut(s) 400, 1379, 1713, 1742, 2692, 3094
Bse1I ACTGG 1 cut(s) 1736
Bse3DI GCAATG 2 cut(s) 669, 2747
BseBI CCWGG 6 cut(s) 1503, 2325, 3164, 3195, 3320, 3464
BseCI ATCGAT 1 cut(s) 132
BseDI CCNNGG 6 cut(s) 1116, 1502, 3163, 3194, 3319, 3463
BseGI GGATG 7 cut(s) 484, 1249, 1328, 1820, 2041, 2045, 2723
BseLI CCNNNNNNNGG 6 cut(s) 400, 1379, 1713, 1742, 2692, 3094
BseMI GCAATG 2 cut(s) 669, 2747
BseMII CTCAG 7 cut(s) 160, 1623, 2308, 2598, 2601, 3062, 3432
BseNI ACTGG 1 cut(s) 1736
BseRI GAGGAG 2 cut(s) 3706, 3709
BseXI GCAGC 8 cut(s) 159, 1703, 2130, 2340, 3265, 3340, 3581, 3748
BseYI CCCAGC 1 cut(s) 456
Bsh1236I CGCG 2 cut(s) 1019, 3585
BshFI GGCC 6 cut(s) 1637, 1717, 1746, 2277, 3518, 3539
BshNI GGYRCC 3 cut(s) 301, 2734, 3747
BshVI ATCGAT 1 cut(s) 132
BsiHKAI GWGCWC 4 cut(s) 103, 1408, 2421, 2588
BsiHKCI CYCGRG 1 cut(s) 1997
BslFI GGGAC 2 cut(s) 2245, 3180
BslI CCNNNNNNNGG 6 cut(s) 400, 1379, 1713, 1742, 2692, 3094
BsmAI GTCTC 9 cut(s) 998, 1083, 1316, 1761, 1805, 2435, 2613, 3192, 3691
BsmFI GGGAC 2 cut(s) 2245, 3180
BsmI GAATGC 3 cut(s) 1586, 2121, 2331
BsnI GGCC 6 cut(s) 1637, 1717, 1746, 2277, 3518, 3539
BsoBI CYCGRG 1 cut(s) 1997
Bsp1286I GDGCHC 5 cut(s) 103, 147, 1408, 2421, 2588
Bsp19I CCATGG 1 cut(s) 1116
BspANI GGCC 6 cut(s) 1637, 1717, 1746, 2277, 3518, 3539
BspCNI CTCAG 7 cut(s) 159, 1624, 2307, 2599, 2600, 3061, 3431
BspDI ATCGAT 1 cut(s) 132
BspFNI CGCG 2 cut(s) 1019, 3585
BspHI TCATGA 1 cut(s) 1786
BspLI GGNNCC 4 cut(s) 303, 2736, 3168, 3749
BspMAI CTGCAG 3 cut(s) 1693, 2816, 2879
BspPI GGATC 4 cut(s) 317, 453, 523, 3069
BspQI GCTCTTC 1 cut(s) 2064
BspT107I GGYRCC 3 cut(s) 301, 2734, 3747
BsrBI CCGCTC 1 cut(s) 428
BsrDI GCAATG 2 cut(s) 669, 2747
BsrI ACTGG 1 cut(s) 1736
BssECI CCNNGG 6 cut(s) 1116, 1502, 3163, 3194, 3319, 3463
BssSI CACGAG 1 cut(s) 1759
BssT1I CCWWGG 1 cut(s) 1116
Bst2BI CACGAG 1 cut(s) 1759
Bst2UI CCWGG 6 cut(s) 1503, 2325, 3164, 3195, 3320, 3464
Bst4CI ACNGT 5 cut(s) 1358, 2540, 2669, 3031, 3278
Bst6I CTCTTC 6 cut(s) 238, 399, 2064, 3177, 3195, 3770
BstC8I GCNNGC 6 cut(s) 258, 488, 1017, 1715, 2950, 3516
BstDSI CCRYGG 1 cut(s) 1116
BstENI CCTNNNNNAGG 1 cut(s) 398
BstF5I GGATG 7 cut(s) 484, 1249, 1328, 1820, 2041, 2045, 2723
BstFNI CGCG 2 cut(s) 1019, 3585
BstH2I RGCGCY 1 cut(s) 1148
BstHHI GCGC 4 cut(s) 1147, 1512, 3253, 3305
BstMAI GTCTC 9 cut(s) 998, 1083, 1316, 1761, 1805, 2435, 2613, 3192, 3691
BstMWI GCNNNNNNNGC 5 cut(s) 1144, 1571, 3302, 3524, 3591
BstNI CCWGG 6 cut(s) 1503, 2325, 3164, 3195, 3320, 3464
BstNSI RCATGY 5 cut(s) 938, 1311, 1444, 3460, 3649
BstPAI GACNNNNGTC 1 cut(s) 2814
BstSCI CCNGG 6 cut(s) 1501, 2323, 3162, 3193, 3318, 3462
BstSFI CTRYAG 4 cut(s) 608, 1689, 2812, 2875
BstUI CGCG 2 cut(s) 1019, 3585
BstV1I GCAGC 8 cut(s) 159, 1703, 2130, 2340, 3265, 3340, 3581, 3748
BstV2I GAAGAC 4 cut(s) 34, 69, 2663, 2844
BstX2I RGATCY 4 cut(s) 75, 322, 445, 2215
BstXI CCANNNNNNTGG 3 cut(s) 268, 559, 3267
BstYI RGATCY 4 cut(s) 75, 322, 445, 2215
Bsu15I ATCGAT 1 cut(s) 132
BsuRI GGCC 6 cut(s) 1637, 1717, 1746, 2277, 3518, 3539
BsuTUI ATCGAT 1 cut(s) 132
BtgI CCRYGG 1 cut(s) 1116
BtgZI GCGATG 1 cut(s) 3727
BtsCI GGATG 7 cut(s) 484, 1249, 1328, 1820, 2041, 2045, 2723
BtsIMutI CAGTG 4 cut(s) 2302, 2855, 3249, 3426
Cac8I GCNNGC 6 cut(s) 258, 488, 1017, 1715, 2950, 3516
CaiI CAGNNNCTG 2 cut(s) 2544, 3594
CciI TCATGA 1 cut(s) 1786
CfoI GCGC 4 cut(s) 1147, 1512, 3253, 3305
Cfr13I GGNCC 9 cut(s) 423, 1326, 2038, 2290, 2557, 2638, 3167, 3493, 3517
ClaI ATCGAT 1 cut(s) 132
CseI GACGC 1 cut(s) 3574
Csp6I GTAC 4 cut(s) 764, 2591, 3052, 3098
CviQI GTAC 4 cut(s) 764, 2591, 3052, 3098
DraI TTTAAA 2 cut(s) 520, 2085
DraIII CACNNNGTG 2 cut(s) 243, 992
EaeI YGGCCR 2 cut(s) 1715, 1744
Eam1104I CTCTTC 6 cut(s) 238, 399, 2064, 3177, 3195, 3770
EarI CTCTTC 6 cut(s) 238, 399, 2064, 3177, 3195, 3770
EciI GGCGGA 2 cut(s) 1469, 2570
Ecl136II GAGCTC 3 cut(s) 101, 1406, 2419
Eco130I CCWWGG 1 cut(s) 1116
Eco147I AGGCCT 2 cut(s) 1637, 2277
Eco24I GRGCYC 4 cut(s) 103, 147, 1408, 2421
Eco47I GGWCC 8 cut(s) 423, 1326, 2038, 2290, 2557, 2638, 3167, 3493
Eco47III AGCGCT 1 cut(s) 1146
Eco53kI GAGCTC 3 cut(s) 101, 1406, 2419
Eco57I CTGAAG 6 cut(s) 159, 381, 818, 1051, 1223, 3158
Eco88I CYCGRG 1 cut(s) 1997
EcoICRI GAGCTC 3 cut(s) 101, 1406, 2419
EcoNI CCTNNNNNAGG 1 cut(s) 398
EcoO109I RGGNCCY 3 cut(s) 2638, 3167, 3493
EcoRI GAATTC 2 cut(s) 592, 2768
EcoRII CCWGG 6 cut(s) 1501, 2323, 3162, 3193, 3318, 3462
EcoT14I CCWWGG 1 cut(s) 1116
EcoT22I ATGCAT 3 cut(s) 940, 1446, 1625
EcoT38I GRGCYC 4 cut(s) 103, 147, 1408, 2421
ErhI CCWWGG 1 cut(s) 1116
FalI AAGNNNNNCTT 2 cut(s) 906, 938
FaqI GGGAC 2 cut(s) 2245, 3180
FauI CCCGC 1 cut(s) 1024
FauNDI CATATG 3 cut(s) 1730, 2127, 2337
FblI GTMKAC 2 cut(s) 607, 1437
Fnu4HI GCNGC 8 cut(s) 173, 1692, 2119, 2329, 3254, 3354, 3595, 3737
FokI GGATG 7 cut(s) 491, 1256, 1335, 1827, 2028, 2032, 2730
FriOI GRGCYC 4 cut(s) 103, 147, 1408, 2421
Fsp4HI GCNGC 8 cut(s) 173, 1692, 2119, 2329, 3254, 3354, 3595, 3737
FspBI CTAG 7 cut(s) 675, 857, 972, 1271, 1298, 1484, 2435
FspI TGCGCA 1 cut(s) 3252
GlaI GCGC 4 cut(s) 1146, 1511, 3252, 3304
GluI GCNGC 8 cut(s) 173, 1692, 2119, 2329, 3254, 3354, 3595, 3737
GsaI CCCAGC 1 cut(s) 460
GsuI CTGGAG 3 cut(s) 439, 2794, 3074
HaeII RGCGCY 1 cut(s) 1148
HaeIII GGCC 6 cut(s) 1637, 1717, 1746, 2277, 3518, 3539
HgaI GACGC 1 cut(s) 3574
HhaI GCGC 4 cut(s) 1147, 1512, 3253, 3305
Hin6I GCGC 4 cut(s) 1145, 1510, 3251, 3303
HinP1I GCGC 4 cut(s) 1145, 1510, 3251, 3303
HincII GTYRAC 3 cut(s) 1438, 1615, 2211
HindII GTYRAC 3 cut(s) 1438, 1615, 2211
HindIII AAGCTT 3 cut(s) 363, 1258, 2946
HpaI GTTAAC 1 cut(s) 2211
HphI GGTGA 2 cut(s) 625, 956
Hpy166II GTNNAC 8 cut(s) 608, 1438, 1615, 1751, 2030, 2211, 3219, 3670
Hpy8I GTNNAC 8 cut(s) 608, 1438, 1615, 1751, 2030, 2211, 3219, 3670
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 7 cut(s) 134, 136, 793, 1075, 1279, 1898, 3500
HpyCH4III ACNGT 5 cut(s) 1358, 2540, 2669, 3031, 3278
HpyCH4IV ACGT 4 cut(s) 1423, 1940, 2476, 2646
HpyF10VI GCNNNNNNNGC 5 cut(s) 1144, 1571, 3302, 3524, 3591
HpySE526I ACGT 4 cut(s) 1423, 1940, 2476, 2646
HspAI GCGC 4 cut(s) 1145, 1510, 3251, 3303
KspAI GTTAAC 1 cut(s) 2211
LguI GCTCTTC 1 cut(s) 2064
LmnI GCTCC 2 cut(s) 2416, 3261
Lsp1109I GCAGC 8 cut(s) 159, 1703, 2130, 2340, 3265, 3340, 3581, 3748
LweI GCATC 5 cut(s) 1525, 1561, 1805, 2941, 2992
MaeI CTAG 7 cut(s) 675, 857, 972, 1271, 1298, 1484, 2435
MaeII ACGT 4 cut(s) 1423, 1940, 2476, 2646
MbiI CCGCTC 1 cut(s) 428
MfeI CAATTG 1 cut(s) 2469
MflI RGATCY 4 cut(s) 75, 322, 445, 2215
MhlI GDGCHC 5 cut(s) 103, 147, 1408, 2421, 2588
MlsI TGGCCA 1 cut(s) 1717
MluNI TGGCCA 1 cut(s) 1717
MlyI GAGTC 5 cut(s) 823, 966, 1546, 2094, 3410
MmeI TCCRAC 5 cut(s) 390, 544, 613, 815, 2802
Mox20I TGGCCA 1 cut(s) 1717
Mph1103I ATGCAT 3 cut(s) 940, 1446, 1625
MroXI GAANNNNTTC 2 cut(s) 571, 2529
MscI TGGCCA 1 cut(s) 1717
MslI CAYNNNNRTG 4 cut(s) 2126, 2336, 2707, 3745
Msp20I TGGCCA 1 cut(s) 1717
MspA1I CMGCKG 1 cut(s) 3094
MspR9I CCNGG 6 cut(s) 1503, 2325, 3164, 3195, 3320, 3464
MunI CAATTG 1 cut(s) 2469
Mva1269I GAATGC 3 cut(s) 1586, 2121, 2331
MvaI CCWGG 6 cut(s) 1503, 2325, 3164, 3195, 3320, 3464
MvnI CGCG 2 cut(s) 1019, 3585
MwoI GCNNNNNNNGC 5 cut(s) 1144, 1571, 3302, 3524, 3591
NcoI CCATGG 1 cut(s) 1116
NdeI CATATG 3 cut(s) 1730, 2127, 2337
NlaIV GGNNCC 4 cut(s) 303, 2736, 3168, 3749
NmuCI GTSAC 5 cut(s) 1339, 1883, 1946, 2807, 2902
NsbI TGCGCA 1 cut(s) 3252
NsiI ATGCAT 3 cut(s) 940, 1446, 1625
NspI RCATGY 5 cut(s) 938, 1311, 1444, 3460, 3649
OliI CACNNNNGTG 1 cut(s) 3745
PaeR7I CTCGAG 1 cut(s) 1997
PagI TCATGA 1 cut(s) 1786
PceI AGGCCT 2 cut(s) 1637, 2277
PciI ACATGT 1 cut(s) 3456
PciSI GCTCTTC 1 cut(s) 2064
PcsI WCGNNNNNNNCGW 2 cut(s) 1092, 1434
PctI GAATGC 3 cut(s) 1586, 2121, 2331
PdmI GAANNNNTTC 2 cut(s) 571, 2529
PflMI CCANNNNNTGG 1 cut(s) 1742
PkrI GCNGC 8 cut(s) 174, 1693, 2120, 2330, 3255, 3355, 3596, 3738
PleI GAGTC 5 cut(s) 822, 965, 1546, 2094, 3410
PpsI GAGTC 5 cut(s) 822, 965, 1546, 2094, 3410
PpuMI RGGWCCY 3 cut(s) 2638, 3167, 3493
PscI ACATGT 1 cut(s) 3456
PshAI GACNNNNGTC 1 cut(s) 2814
PshBI ATTAAT 3 cut(s) 2910, 3327, 3432
PsiI TTATAA 1 cut(s) 749
Psp124BI GAGCTC 3 cut(s) 103, 1408, 2421
Psp5II RGGWCCY 3 cut(s) 2638, 3167, 3493
Psp6I CCWGG 6 cut(s) 1501, 2323, 3162, 3193, 3318, 3462
PspFI CCCAGC 1 cut(s) 456
PspGI CCWGG 6 cut(s) 1501, 2323, 3162, 3193, 3318, 3462
PspN4I GGNNCC 4 cut(s) 303, 2736, 3168, 3749
PspPI GGNCC 9 cut(s) 423, 1326, 2038, 2290, 2557, 2638, 3167, 3493, 3517
PspPPI RGGWCCY 3 cut(s) 2638, 3167, 3493
PstI CTGCAG 3 cut(s) 1693, 2816, 2879
PstNI CAGNNNCTG 2 cut(s) 2544, 3594
PsuI RGATCY 4 cut(s) 75, 322, 445, 2215
PvuII CAGCTG 1 cut(s) 3094
RsaI GTAC 4 cut(s) 765, 2592, 3053, 3099
RsaNI GTAC 4 cut(s) 764, 2591, 3052, 3098
RseI CAYNNNNRTG 4 cut(s) 2126, 2336, 2707, 3745
SacI GAGCTC 3 cut(s) 103, 1408, 2421
SalI GTCGAC 1 cut(s) 1436
SapI GCTCTTC 1 cut(s) 2064
SatI GCNGC 8 cut(s) 173, 1692, 2119, 2329, 3254, 3354, 3595, 3737
Sau96I GGNCC 9 cut(s) 423, 1326, 2038, 2290, 2557, 2638, 3167, 3493, 3517
ScaI AGTACT 1 cut(s) 2592
SchI GAGTC 5 cut(s) 823, 966, 1546, 2094, 3410
ScrFI CCNGG 6 cut(s) 1503, 2325, 3164, 3195, 3320, 3464
SduI GDGCHC 5 cut(s) 103, 147, 1408, 2421, 2588
SfaNI GCATC 5 cut(s) 1525, 1561, 1805, 2941, 2992
SfcI CTRYAG 4 cut(s) 608, 1689, 2812, 2875
Sfr274I CTCGAG 1 cut(s) 1997
SinI GGWCC 8 cut(s) 423, 1326, 2038, 2290, 2557, 2638, 3167, 3493
SlaI CTCGAG 1 cut(s) 1997
SmiMI CAYNNNNRTG 4 cut(s) 2126, 2336, 2707, 3745
SmlI CTYRAG 8 cut(s) 193, 1043, 1105, 1128, 1997, 2033, 2193, 2420
SmoI CTYRAG 8 cut(s) 193, 1043, 1105, 1128, 1997, 2033, 2193, 2420
SseBI AGGCCT 2 cut(s) 1637, 2277
SspMI CTAG 7 cut(s) 675, 857, 972, 1271, 1298, 1484, 2435
SstI GAGCTC 3 cut(s) 103, 1408, 2421
StuI AGGCCT 2 cut(s) 1637, 2277
StyD4I CCNGG 6 cut(s) 1501, 2323, 3162, 3193, 3318, 3462
StyI CCWWGG 1 cut(s) 1116
TaaI ACNGT 5 cut(s) 1358, 2540, 2669, 3031, 3278
TaiI ACGT 4 cut(s) 1426, 1943, 2479, 2649
TaqII GACCGA 1 cut(s) 74
TatI WGTACW 2 cut(s) 763, 2590
TscAI CASTG 4 cut(s) 2302, 2862, 3249, 3426
TseFI GTSAC 5 cut(s) 1339, 1883, 1946, 2807, 2902
TseI GCWGC 8 cut(s) 172, 1691, 2118, 2328, 3253, 3353, 3594, 3736
Tsp45I GTSAC 5 cut(s) 1339, 1883, 1946, 2807, 2902
TspGWI ACGGA 4 cut(s) 352, 1101, 2429, 3074
TspRI CASTG 4 cut(s) 2302, 2862, 3249, 3426
Van91I CCANNNNNTGG 1 cut(s) 1742
VpaK11BI GGWCC 8 cut(s) 423, 1326, 2038, 2290, 2557, 2638, 3167, 3493
VspI ATTAAT 3 cut(s) 2910, 3327, 3432
XagI CCTNNNNNAGG 1 cut(s) 398
XbaI TCTAGA 2 cut(s) 1297, 2434
XceI RCATGY 5 cut(s) 938, 1311, 1444, 3460, 3649
XhoI CTCGAG 1 cut(s) 1997
XmiI GTMKAC 2 cut(s) 607, 1437
XmnI GAANNNNTTC 2 cut(s) 571, 2529
XspI CTAG 7 cut(s) 675, 857, 972, 1271, 1298, 1484, 2435
ZrmI AGTACT 1 cut(s) 2592
Zsp2I ATGCAT 3 cut(s) 940, 1446, 1625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.