Rroxscaffold_4G00282520

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000067
Physical Location & Seq
Reverse (-)
4573300 .. 4579092
5793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00282520.1

Sequence Viewer

Length: 3360 bp
ATGGATTCCCACATTACAGAAATGAATTCAAGATTATGTCTTCAGGTGAATGATGTTCGTGTTGTTGGAATATGGGGTATGGGCGGTTTAGGAAAAACAACCATTGCTAGAGCTGTTTTTGACGAAATTGCTTGCCAATTTGAGCACTCCTGCTTTCTTGATAACGTCAAAGAAGCTTTTATAAACAAGCGTGAAGTACAAATGCAGGAAGCACTTATATCTATATTGCTGACGGAAAAGGTGCAGAGTCTGGGTTTGAACAGAGGTTGGAATATGATAAAGGAAAGGCTAGGTATGAAAAAAGTCTTGGTTGTTCTTGATGATGTGGAAACTTTTGCCCAAATAGAAGCCTTGCTTGGAAAACTACATGCATTTGGTGAGGGAAGCAGACTCATTGTAACAACTAGAAATAAGCAATCGCTAAGTGGAGTTAATGAGACGTATAAGCCATCGTATTTAAGGGATGATGAAGCTCTCGAGCTGTTTCGGAAATATGCTTTCAGAACAAATCAACCCAACAGAGACTACGATCATCTATCAAGGCGTGCCATAGAATATGCTCAAGGTTTGCCTTTAGCACTTAAAGTCTTGGGGGCATTTCTTGATAACAAAAGTGTATGTGAATGGGAAGTCGTGTTGGAGAAATTGAAGAAAATCCCACACATAGAAATTCAGGATGTGCTTAGAACAAGCTTTGATGGACTAGACGATTCAGAGAAAAACATATTCCTAGATATTGCATGTCACTTTAGAAAATGGCGCACAAATTTTGTAACGGAATTACTGGACGGTTTTGGCTTCTTTCCCCACAATGGATTAAGAGTTCTAGTTGACAGAGCTCTCATAACTTCGTCAAATTACAGAGTCGACATGCATGATTTATTACAAGAAATGGGTAGAGAAATCGTCCGCCTAGAATCTGCTAAAAAACCTGGAAAGCGTAGTAGGTTGTGGAGTTATAAAGATGTTAATCACGTGTTGACTCAAAATACGGCTACGGATGCAGTTGAATGCTTAATGCTAGACTTGTCGAACTCAAAAGTTGACCTAAGCATAAATACTGAGGCCTTTGTAAGGATGACGAAGCTAAGACTGCTAATAATCTGCTATTCATTCCACATATACCTTTTTCCATATAGCGAGTTCCGCAAGAATATATGCCCAGTAGGTGGCTGTAAACAACTCTTGAGGGGGAACTTCGAGTTTCTTTCCCATGAATTGAGGATTCTCTTATGGCATGGATGCCCGCTAAAGTGTTTGCCATCAACTTTTATCCCAAAGAATCTTGTTCACCTTGACATGCGTGGTAGTCACATTCGAGAACTTTGGGAAGGAATCAAGCCCCTACAAAATCTGAGAGTCATCAAGTTAAGTCACTGTCGATACCTTCTCAAAATCCCCGACTTAACTGAGGCGATAAATCTTGAGACGCTACTTCTTGATGGCTGTTCAAGTTTATTTGAGGTTCATTCATCCATTTCTGCTCTTCAAAACCTTCATTTATTGGATCTAACCGGGTGCAAAAAACTCAAGATTCTCCCAAAGAGAATTCATATGAAATCTCTACAGACCCTTTGTCTTTCGGGCTGCTCAAATCTTGAAAATTTTCCTGAGATTTCAGGAATCATGAAGGAGCTATCAATGCTTCGTTTAGATGGGACCGCAATTAAAGGACTGTCCTCATCAATTGATCTGCTTCTGAGGATTAAAAAATTAAGTGTGCGGAACTGCAAAAGCCTTGAATTTCTTCCCGACAGTATCTGTAATTTGGCAAACCTTACACATCTGAATCTGTCTGGGTGCTCGGTACTTCATTACTTGCCTGAGAAATTGGGGGACTTAAAATCATTAGAGGTCCTCGATGTAGGATATAGTGGTATAAAACAACTTCCATTCTCTATCCTACGTTTGGGAAAGCTTAAAGGTGGATTATCATGTATTGGATGTAAAAAGATGACGGCACCCTTGTCAGCCTGGCAATCATCAATTGAAGAATATTGTAGTTACTCTGTTCTGCTACGTCTTGATTTAAGTGACTGCAATCTGTTGGAGTTATCGGATGGTATTGCTCATTTATATTCATTGAAAACATTAAAGCTGTGCAGAAACAATTTGGAGAGCTTACCTGTGACAATGAATCGACTTCGTTGTCTAACGCATCTTGAATTGGAAGCTTGTAAGCGACTGAAGTCGGTACCTGAGCTTTCATCGAGTGTAATACGCATAGATGCACATGATTGCACAGCTCTGGAAACAGTTTCAAGACGAAAGACTGATCCAGTATGTAGAATTCTAGACTTCAGGTTTTCTAATTGCTTCCGGCTTCAAGCAAATTTGTTTATAGATATTGTTGAGACTGAGATTTATTCTCTCGACCAGGACAGTCATCTGCGACCTCTGTGGTTCCACATGTGGCTTCCTGGAAGTGAAGTTCCAGAATGGTTCAACCTTCGGGATACAGGATGTTCAGAAACTTTTCGGAAGACAGGATGTTCAGCAACTTTCCAAGCACCACTGAATAGTTTCAATAACAAGCTTTTAGGATTTGCTATATGCATTGTTATTAAATTCAATGGTGCTCGTCATGATGTATCTGATATGTATGGAAGATGTTATTGGACCTCAAAAGGAAAACATGGTGAGTACTCCTTCGAATTTGATTTGCTCGGCCGGCAGTTCGGAATTTACAGTATAGAGTCAGATCACCTGTTCATGGCATATTTGCCATGGTCTGGATATGGCAGGATTTCACAAGATAGATTAGATGCATTCAAGATTGTAGTAGAAACTGGAACTTATTCTCCGACAGAAGATTGGTTTACTAGTGGCTGCGGTGTTCGTTTAGTTTATGCTCACGATGAGATGCAAAATAGCGTGACACAGCCCCTAATTCATGTGGAAGGCCAGACAATGGAGGATGCGGTGCAGCAGGGGGAGGGGTGCTGTGCTATAGGAGGGGTGTCGGGGCTTGGCGCACCTTTCTTTACGGACCAGTTGGAAATAAGAAAGAGTGAAACAGAGTTGGAGGCAGAGTCATCGGAGTCGCGTGAGGGGAGAGGCACGTGCCAAATATTGGAGCTGTTACCAGAGCCGCAGACTGAGAGAAAAGGAGAGGAGGATGGGGAGGGGTGTCAGGTGTTAGGGTCGGCATTCTCTTTGAGAAAGAGAGAAACAGAAAGAGAAGGAGACAGAGTCACAAGAGTCTCTGACGAGGGAGAGATATTGGAGTTGGGCAGCCACAGTGGTCTGGCGGTGCCAAATTCACAGAGTGAGGGCAAAGAAGAGAAAGAATTGATGGGGGAGGAGTCGTTGGAGAGGGTTGTTAGGGCCGCTGTGCAGCTGCTTTTACTGGCTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1119

Amino Acids

126.6

Weight (kDa)

6.27

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 5 - 170 5.9e-20 NB-ARC domain
WHD_ROQ1 PF23282 238 - 306 1.5e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 488 - 599 1e-05 Leucine-rich repeat region
LRR_13 PF23286 519 - 604 6.3e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 573 - 641 3.9e-09 Leucine-rich repeat region
LRR_14 PF23598 670 - 730 9.8e-06 Leucine-rich repeat region
LRR_8 PF13855 674 - 726 1.7e-06 Leucine rich repeat
C-JID PF20160 806 - 953 9.7e-10 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 182, 960
AasI GACNNNNNNGTC 1 cut(s) 2148
Acc65I GGTACC 1 cut(s) 2194
AccB1I GGYRCC 3 cut(s) 1960, 2194, 3253
AccB7I CCANNNNNTGG 4 cut(s) 1169, 2732, 2911, 3073
AccI GTMKAC 1 cut(s) 867
AccII CGCG 1 cut(s) 3046
AclWI GGATC 2 cut(s) 1515, 2270
AcoI YGGCCR 1 cut(s) 2668
AcuI CTGAAG 3 cut(s) 26, 2207, 2284
AcvI CACGTG 2 cut(s) 976, 3063
AdeI CACNNNGTG 1 cut(s) 3269
AfaI GTAC 4 cut(s) 198, 1809, 2196, 2645
AfiI CCNNNNNNNGG 8 cut(s) 812, 1074, 1169, 1909, 2713, 2732, 2911, 3073
AflIII ACRYGT 2 cut(s) 975, 2409
AhdI GACNNNNNGTC 1 cut(s) 1575
AhlI ACTAGT 1 cut(s) 2822
AjnI CCWGG 4 cut(s) 931, 1973, 2376, 2419
AjuI GAANNNNNNNTTGG 6 cut(s) 290, 322, 339, 371, 2599, 2631
AloI GAACNNNNNNTCC 6 cut(s) 807, 839, 2415, 2447, 2785, 2817
Alw21I GWGCWC 4 cut(s) 147, 841, 1805, 2581
Alw26I GTCTC 6 cut(s) 431, 516, 1421, 2348, 3180, 3208
AlwI GGATC 2 cut(s) 1515, 2270
AlwNI CAGNNNCTG 2 cut(s) 250, 1761
Ama87I CYCGRG 1 cut(s) 476
AoxI GGCC 4 cut(s) 1065, 2668, 2902, 3327
ApeKI GCWGC 6 cut(s) 1587, 2829, 2926, 3234, 3337, 3340
Asp700I GAANNNNTTC 5 cut(s) 1605, 1746, 2475, 2522, 2797
Asp718I GGTACC 1 cut(s) 2194
AspLEI GCGC 2 cut(s) 762, 2975
AspS9I GGNCC 5 cut(s) 1659, 1855, 2619, 2989, 3327
AsuC2I CCSGG 1 cut(s) 1516
AsuHPI GGTGA 5 cut(s) 58, 389, 1283, 2651, 2696
AsuII TTCGAA 1 cut(s) 2652
AvaI CYCGRG 1 cut(s) 476
AvaII GGWCC 4 cut(s) 1659, 1855, 2619, 2989
BanI GGYRCC 3 cut(s) 1960, 2194, 3253
BanII GRGCYC 1 cut(s) 841
BbrPI CACGTG 2 cut(s) 976, 3063
BbsI GAAGAC 2 cut(s) 32, 2489
Bbv12I GWGCWC 4 cut(s) 147, 841, 1805, 2581
BbvI GCAGC 6 cut(s) 1574, 2816, 2938, 3246, 3327, 3349
BccI CCATC 8 cut(s) 457, 692, 1270, 1436, 1649, 2054, 3113, 3289
BceAI ACGGC 2 cut(s) 1008, 1974
BcgI CGANNNNNNTGC 2 cut(s) 3018, 3052
BciT130I CCWGG 4 cut(s) 933, 1975, 2378, 2421
BciVI GTATCC 1 cut(s) 2449
BcnI CCSGG 1 cut(s) 1516
BcoDI GTCTC 6 cut(s) 431, 516, 1421, 2348, 3180, 3208
BcuI ACTAGT 1 cut(s) 2822
BfmI CTRYAG 2 cut(s) 1565, 2949
BfuI GTATCC 1 cut(s) 2449
BisI GCNGC 8 cut(s) 1588, 2830, 2927, 3092, 3235, 3330, 3338, 3341
BlsI GCNGC 8 cut(s) 1589, 2831, 2928, 3093, 3236, 3331, 3339, 3342
BmcAI AGTACT 1 cut(s) 2645
Bme1390I CCNGG 5 cut(s) 933, 1516, 1975, 2378, 2421
Bme18I GGWCC 4 cut(s) 1659, 1855, 2619, 2989
BmeRI GACNNNNNGTC 1 cut(s) 1575
BmeT110I CYCGRG 1 cut(s) 476
BmgT120I GGNCC 5 cut(s) 1659, 1855, 2619, 2989, 3327
BmiI GGNNCC 5 cut(s) 1660, 1962, 2196, 2405, 3255
BmrFI CCNGG 5 cut(s) 933, 1516, 1975, 2378, 2421
BmrI ACTGGG 1 cut(s) 1157
BmsI GCATC 7 cut(s) 991, 1232, 2167, 2218, 2755, 2853, 2908
BmuI ACTGGG 1 cut(s) 1157
BoxI GACNNNNGTC 1 cut(s) 2188
BpiI GAAGAC 2 cut(s) 32, 2489
Bpu10I CCTNAGC 2 cut(s) 1049, 2199
Bpu14I TTCGAA 1 cut(s) 2652
BpuEI CTTGAG 4 cut(s) 546, 1207, 1445, 1514
BpuMI CCSGG 1 cut(s) 1516
BsaAI YACGTR 2 cut(s) 976, 3063
BsaBI GATNNNNATC 1 cut(s) 969
BsaJI CCNNGG 1 cut(s) 2726
BsaXI ACNNNNNCTCC 8 cut(s) 2785, 2815, 3017, 3047, 3177, 3207, 3293, 3323
Bsc4I CCNNNNNNNGG 8 cut(s) 812, 1074, 1169, 1909, 2713, 2732, 2911, 3073
Bse118I RCCGGY 1 cut(s) 2670
Bse1I ACTGG 6 cut(s) 789, 1163, 2279, 2794, 2992, 3354
Bse3DI GCAATG 1 cut(s) 102
Bse8I GATNNNNATC 1 cut(s) 969
BseBI CCWGG 4 cut(s) 933, 1975, 2378, 2421
BseDI CCNNGG 1 cut(s) 2726
BseJI GATNNNNATC 1 cut(s) 969
BseLI CCNNNNNNNGG 8 cut(s) 812, 1074, 1169, 1909, 2713, 2732, 2911, 3073
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 9 cut(s) 1053, 1346, 1401, 1602, 1691, 1815, 2190, 2349, 3090
BseNI ACTGG 6 cut(s) 789, 1163, 2279, 2794, 2992, 3354
BseRI GAGGAG 2 cut(s) 3128, 3317
BseX3I CGGCCG 1 cut(s) 2668
BseXI GCAGC 6 cut(s) 1574, 2816, 2938, 3246, 3327, 3349
BseYI CCCAGC 1 cut(s) 3353
BsgI GTGCAG 4 cut(s) 263, 2122, 2945, 3356
Bsh1236I CGCG 1 cut(s) 3046
Bsh1285I CGRYCG 1 cut(s) 2671
BshFI GGCC 4 cut(s) 1067, 2670, 2904, 3329
BshNI GGYRCC 3 cut(s) 1960, 2194, 3253
BsiEI CGRYCG 1 cut(s) 2671
BsiHKAI GWGCWC 4 cut(s) 147, 841, 1805, 2581
BsiHKCI CYCGRG 1 cut(s) 476
BsiSI CCGG 3 cut(s) 1515, 2320, 2671
BslFI GGGAC 2 cut(s) 1672, 1850
BslI CCNNNNNNNGG 8 cut(s) 812, 1074, 1169, 1909, 2713, 2732, 2911, 3073
BsmAI GTCTC 6 cut(s) 431, 516, 1421, 2348, 3180, 3208
BsmBI CGTCTC 2 cut(s) 431, 1421
BsmFI GGGAC 2 cut(s) 1672, 1850
BsmI GAATGC 3 cut(s) 1016, 2768, 3149
BsnI GGCC 4 cut(s) 1067, 2670, 2904, 3329
BsoBI CYCGRG 1 cut(s) 476
Bsp119I TTCGAA 1 cut(s) 2652
Bsp1286I GDGCHC 4 cut(s) 147, 841, 1805, 2581
Bsp143I GATC 5 cut(s) 529, 1507, 1690, 2275, 2701
Bsp19I CCATGG 1 cut(s) 2726
BspANI GGCC 4 cut(s) 1067, 2670, 2904, 3329
BspCNI CTCAG 9 cut(s) 1054, 1347, 1402, 1603, 1692, 1816, 2191, 2350, 3091
BspFNI CGCG 1 cut(s) 3046
BspHI TCATGA 2 cut(s) 1626, 2584
BspLI GGNNCC 5 cut(s) 1660, 1962, 2196, 2405, 3255
BspPI GGATC 2 cut(s) 1515, 2270
BspQI GCTCTTC 1 cut(s) 1491
BspT104I TTCGAA 1 cut(s) 2652
BspT107I GGYRCC 3 cut(s) 1960, 2194, 3253
BsrDI GCAATG 1 cut(s) 102
BsrFI RCCGGY 1 cut(s) 2670
BsrI ACTGG 6 cut(s) 789, 1163, 2279, 2794, 2992, 3354
BssAI RCCGGY 1 cut(s) 2670
BssECI CCNNGG 1 cut(s) 2726
BssMI GATC 5 cut(s) 529, 1507, 1690, 2275, 2701
BssT1I CCWWGG 1 cut(s) 2726
Bst2UI CCWGG 4 cut(s) 933, 1975, 2378, 2421
Bst4CI ACNGT 8 cut(s) 791, 1379, 1677, 1757, 2257, 2384, 2690, 3242
Bst6I CTCTTC 2 cut(s) 1491, 3276
BstBAI YACGTR 2 cut(s) 976, 3063
BstBI TTCGAA 1 cut(s) 2652
BstC8I GCNNGC 4 cut(s) 133, 546, 1247, 2672
BstDSI CCRYGG 1 cut(s) 2726
BstENI CCTNNNNNAGG 1 cut(s) 1072
BstFNI CGCG 1 cut(s) 3046
BstHHI GCGC 2 cut(s) 762, 2975
BstKTI GATC 5 cut(s) 532, 1510, 1693, 2278, 2704
BstMAI GTCTC 6 cut(s) 431, 516, 1421, 2348, 3180, 3208
BstMBI GATC 5 cut(s) 529, 1507, 1690, 2275, 2701
BstMCI CGRYCG 1 cut(s) 2671
BstMWI GCNNNNNNNGC 5 cut(s) 1001, 1093, 1146, 1642, 2671
BstNI CCWGG 4 cut(s) 933, 1975, 2378, 2421
BstNSI RCATGY 5 cut(s) 371, 744, 874, 1303, 2413
BstPAI GACNNNNGTC 1 cut(s) 2188
BstSCI CCNGG 5 cut(s) 931, 1514, 1973, 2376, 2419
BstSFI CTRYAG 2 cut(s) 1565, 2949
BstUI CGCG 1 cut(s) 3046
BstV1I GCAGC 6 cut(s) 1574, 2816, 2938, 3246, 3327, 3349
BstV2I GAAGAC 2 cut(s) 32, 2489
BstX2I RGATCY 1 cut(s) 1507
BstYI RGATCY 1 cut(s) 1507
BstZI CGGCCG 1 cut(s) 2668
BsuI GTATCC 1 cut(s) 2449
BsuRI GGCC 4 cut(s) 1067, 2670, 2904, 3329
BtgI CCRYGG 1 cut(s) 2726
BtsIMutI CAGTG 3 cut(s) 1375, 2513, 3247
Cac8I GCNNGC 4 cut(s) 133, 546, 1247, 2672
CaiI CAGNNNCTG 2 cut(s) 250, 1761
CciI TCATGA 2 cut(s) 1626, 2584
CfoI GCGC 2 cut(s) 762, 2975
Cfr10I RCCGGY 1 cut(s) 2670
Cfr13I GGNCC 5 cut(s) 1659, 1855, 2619, 2989, 3327
CseI GACGC 1 cut(s) 1438
Csp6I GTAC 4 cut(s) 197, 1808, 2195, 2644
CviQI GTAC 4 cut(s) 197, 1808, 2195, 2644
DpnI GATC 5 cut(s) 531, 1509, 1692, 2277, 2703
DpnII GATC 5 cut(s) 529, 1507, 1690, 2275, 2701
DraIII CACNNNGTG 1 cut(s) 3269
DrdI GACNNNNNNGTC 1 cut(s) 2148
DriI GACNNNNNGTC 1 cut(s) 1575
DseDI GACNNNNNNGTC 1 cut(s) 2148
EaeI YGGCCR 1 cut(s) 2668
EagI CGGCCG 1 cut(s) 2668
Eam1104I CTCTTC 2 cut(s) 1491, 3276
Eam1105I GACNNNNNGTC 1 cut(s) 1575
EarI CTCTTC 2 cut(s) 1491, 3276
EciI GGCGGA 1 cut(s) 899
Ecl136II GAGCTC 1 cut(s) 839
EclXI CGGCCG 1 cut(s) 2668
Eco130I CCWWGG 1 cut(s) 2726
Eco147I AGGCCT 1 cut(s) 1067
Eco24I GRGCYC 1 cut(s) 841
Eco47I GGWCC 4 cut(s) 1659, 1855, 2619, 2989
Eco52I CGGCCG 1 cut(s) 2668
Eco53kI GAGCTC 1 cut(s) 839
Eco57I CTGAAG 3 cut(s) 26, 2207, 2284
Eco72I CACGTG 2 cut(s) 976, 3063
Eco88I CYCGRG 1 cut(s) 476
EcoICRI GAGCTC 1 cut(s) 839
EcoNI CCTNNNNNAGG 1 cut(s) 1072
EcoO109I RGGNCCY 1 cut(s) 1855
EcoRI GAATTC 3 cut(s) 25, 1548, 2289
EcoRII CCWGG 4 cut(s) 931, 1973, 2376, 2419
EcoT14I CCWWGG 1 cut(s) 2726
EcoT22I ATGCAT 4 cut(s) 373, 876, 2558, 2770
EcoT38I GRGCYC 1 cut(s) 841
ErhI CCWWGG 1 cut(s) 2726
Esp3I CGTCTC 2 cut(s) 431, 1421
FalI AAGNNNNNCTT 2 cut(s) 339, 371
FaqI GGGAC 2 cut(s) 1672, 1850
FauI CCCGC 1 cut(s) 1254
FauNDI CATATG 1 cut(s) 1554
FblI GTMKAC 1 cut(s) 867
Fnu4HI GCNGC 8 cut(s) 1588, 2830, 2927, 3092, 3235, 3330, 3338, 3341
FriOI GRGCYC 1 cut(s) 841
Fsp4HI GCNGC 8 cut(s) 1588, 2830, 2927, 3092, 3235, 3330, 3338, 3341
GlaI GCGC 2 cut(s) 761, 2974
GluI GCNGC 8 cut(s) 1588, 2830, 2927, 3092, 3235, 3330, 3338, 3341
GsaI CCCAGC 1 cut(s) 3357
HaeIII GGCC 4 cut(s) 1067, 2670, 2904, 3329
HapII CCGG 3 cut(s) 1515, 2320, 2671
HgaI GACGC 1 cut(s) 1438
HhaI GCGC 2 cut(s) 762, 2975
Hin6I GCGC 2 cut(s) 760, 2973
HinP1I GCGC 2 cut(s) 760, 2973
HincII GTYRAC 4 cut(s) 832, 868, 981, 1045
HindII GTYRAC 4 cut(s) 832, 868, 981, 1045
HindIII AAGCTT 5 cut(s) 174, 691, 1916, 2172, 2534
HpaII CCGG 3 cut(s) 1515, 2320, 2671
HphI GGTGA 5 cut(s) 58, 389, 1283, 2651, 2696
Hpy166II GTNNAC 7 cut(s) 832, 868, 981, 1045, 1178, 1291, 2820
Hpy8I GTNNAC 7 cut(s) 832, 868, 981, 1045, 1178, 1291, 2820
HpyAV CCTTC 8 cut(s) 1325, 1397, 1505, 1624, 2459, 2659, 2894, 3176
HpyCH4III ACNGT 8 cut(s) 791, 1379, 1677, 1757, 2257, 2384, 2690, 3242
HpyCH4IV ACGT 6 cut(s) 165, 440, 975, 1906, 2020, 3062
HpyF10VI GCNNNNNNNGC 5 cut(s) 1001, 1093, 1146, 1642, 2671
HpySE526I ACGT 6 cut(s) 165, 440, 975, 1906, 2020, 3062
HspAI GCGC 2 cut(s) 760, 2973
KpnI GGTACC 1 cut(s) 2198
KroI GCCGGC 1 cut(s) 2670
KroNI GCCGGC 1 cut(s) 2672
Kzo9I GATC 5 cut(s) 529, 1507, 1690, 2275, 2701
LguI GCTCTTC 1 cut(s) 1491
LmnI GCTCC 2 cut(s) 1633, 3076
Lsp1109I GCAGC 6 cut(s) 1574, 2816, 2938, 3246, 3327, 3349
LweI GCATC 7 cut(s) 991, 1232, 2167, 2218, 2755, 2853, 2908
MaeII ACGT 6 cut(s) 165, 440, 975, 1906, 2020, 3062
MalI GATC 5 cut(s) 531, 1509, 1692, 2277, 2703
MboI GATC 5 cut(s) 529, 1507, 1690, 2275, 2701
MboII GAAGA 9 cut(s) 32, 661, 1478, 1739, 2003, 2494, 2619, 2822, 3293
MfeI CAATTG 2 cut(s) 1686, 1986
MflI RGATCY 1 cut(s) 1507
MhlI GDGCHC 4 cut(s) 147, 841, 1805, 2581
MmeI TCCRAC 8 cut(s) 46, 248, 618, 2028, 2828, 2976, 3003, 3291
Mph1103I ATGCAT 4 cut(s) 373, 876, 2558, 2770
MroNI GCCGGC 1 cut(s) 2670
MroXI GAANNNNTTC 5 cut(s) 1605, 1746, 2475, 2522, 2797
MspA1I CMGCKG 2 cut(s) 3332, 3340
MspI CCGG 3 cut(s) 1515, 2320, 2671
MspR9I CCNGG 5 cut(s) 933, 1516, 1975, 2378, 2421
MunI CAATTG 2 cut(s) 1686, 1986
Mva1269I GAATGC 3 cut(s) 1016, 2768, 3149
MvaI CCWGG 4 cut(s) 933, 1975, 2378, 2421
MvnI CGCG 1 cut(s) 3046
MwoI GCNNNNNNNGC 5 cut(s) 1001, 1093, 1146, 1642, 2671
NaeI GCCGGC 1 cut(s) 2672
NciI CCSGG 1 cut(s) 1516
NcoI CCATGG 1 cut(s) 2726
NdeI CATATG 1 cut(s) 1554
NdeII GATC 5 cut(s) 529, 1507, 1690, 2275, 2701
NgoMIV GCCGGC 1 cut(s) 2670
NlaIV GGNNCC 5 cut(s) 1660, 1962, 2196, 2405, 3255
NmeAIII GCCGAG 1 cut(s) 2646
NmuCI GTSAC 7 cut(s) 743, 1310, 1373, 2033, 2128, 2875, 3193
NsiI ATGCAT 4 cut(s) 373, 876, 2558, 2770
NspI RCATGY 5 cut(s) 371, 744, 874, 1303, 2413
NspV TTCGAA 1 cut(s) 2652
PaeR7I CTCGAG 1 cut(s) 476
PagI TCATGA 2 cut(s) 1626, 2584
PceI AGGCCT 1 cut(s) 1067
PciI ACATGT 1 cut(s) 2409
PciSI GCTCTTC 1 cut(s) 1491
PctI GAATGC 3 cut(s) 1016, 2768, 3149
PdiI GCCGGC 1 cut(s) 2672
PdmI GAANNNNTTC 5 cut(s) 1605, 1746, 2475, 2522, 2797
PflFI GACNNNGTC 1 cut(s) 3191
PflMI CCANNNNNTGG 4 cut(s) 1169, 2732, 2911, 3073
PfoI TCCNGGA 1 cut(s) 2419
PkrI GCNGC 8 cut(s) 1589, 2831, 2928, 3093, 3236, 3331, 3339, 3342
PmaCI CACGTG 2 cut(s) 976, 3063
PmlI CACGTG 2 cut(s) 976, 3063
Ppu21I YACGTR 2 cut(s) 976, 3063
PpuMI RGGWCCY 1 cut(s) 1855
PscI ACATGT 1 cut(s) 2409
PshAI GACNNNNGTC 1 cut(s) 2188
PsiI TTATAA 2 cut(s) 182, 960
Psp124BI GAGCTC 1 cut(s) 841
Psp5II RGGWCCY 1 cut(s) 1855
Psp6I CCWGG 4 cut(s) 931, 1973, 2376, 2419
PspCI CACGTG 2 cut(s) 976, 3063
PspFI CCCAGC 1 cut(s) 3353
PspGI CCWGG 4 cut(s) 931, 1973, 2376, 2419
PspN4I GGNNCC 5 cut(s) 1660, 1962, 2196, 2405, 3255
PspPI GGNCC 5 cut(s) 1659, 1855, 2619, 2989, 3327
PspPPI RGGWCCY 1 cut(s) 1855
PstNI CAGNNNCTG 2 cut(s) 250, 1761
PsuI RGATCY 1 cut(s) 1507
PsyI GACNNNGTC 1 cut(s) 3191
PvuII CAGCTG 1 cut(s) 3340
RsaI GTAC 4 cut(s) 198, 1809, 2196, 2645
RsaNI GTAC 4 cut(s) 197, 1808, 2195, 2644
SacI GAGCTC 1 cut(s) 841
SalI GTCGAC 1 cut(s) 866
SapI GCTCTTC 1 cut(s) 1491
SatI GCNGC 8 cut(s) 1588, 2830, 2927, 3092, 3235, 3330, 3338, 3341
Sau3AI GATC 5 cut(s) 529, 1507, 1690, 2275, 2701
Sau96I GGNCC 5 cut(s) 1659, 1855, 2619, 2989, 3327
ScaI AGTACT 1 cut(s) 2645
ScrFI CCNGG 5 cut(s) 933, 1516, 1975, 2378, 2421
SduI GDGCHC 4 cut(s) 147, 841, 1805, 2581
SfaNI GCATC 7 cut(s) 991, 1232, 2167, 2218, 2755, 2853, 2908
SfcI CTRYAG 2 cut(s) 1565, 2949
Sfr274I CTCGAG 1 cut(s) 476
SfuI TTCGAA 1 cut(s) 2652
SinI GGWCC 4 cut(s) 1659, 1855, 2619, 2989
SlaI CTCGAG 1 cut(s) 476
SmlI CTYRAG 5 cut(s) 476, 561, 1186, 1424, 1529
SmoI CTYRAG 5 cut(s) 476, 561, 1186, 1424, 1529
SpeI ACTAGT 1 cut(s) 2822
SseBI AGGCCT 1 cut(s) 1067
SspI AATATT 2 cut(s) 1997, 3072
SstI GAGCTC 1 cut(s) 841
StuI AGGCCT 1 cut(s) 1067
StyD4I CCNGG 5 cut(s) 931, 1514, 1973, 2376, 2419
StyI CCWWGG 1 cut(s) 2726
TaaI ACNGT 8 cut(s) 791, 1379, 1677, 1757, 2257, 2384, 2690, 3242
TaiI ACGT 6 cut(s) 168, 443, 978, 1909, 2023, 3065
TatI WGTACW 2 cut(s) 196, 2643
TauI GCSGC 2 cut(s) 3094, 3332
TscAI CASTG 3 cut(s) 1382, 2520, 3247
TseFI GTSAC 7 cut(s) 743, 1310, 1373, 2033, 2128, 2875, 3193
TseI GCWGC 6 cut(s) 1587, 2829, 2926, 3234, 3337, 3340
Tsp45I GTSAC 7 cut(s) 743, 1310, 1373, 2033, 2128, 2875, 3193
TspGWI ACGGA 4 cut(s) 248, 791, 1013, 3002
TspRI CASTG 3 cut(s) 1382, 2520, 3247
Tth111I GACNNNGTC 1 cut(s) 3191
Van91I CCANNNNNTGG 4 cut(s) 1169, 2732, 2911, 3073
VpaK11BI GGWCC 4 cut(s) 1659, 1855, 2619, 2989
XagI CCTNNNNNAGG 1 cut(s) 1072
XbaI TCTAGA 1 cut(s) 2293
XceI RCATGY 5 cut(s) 371, 744, 874, 1303, 2413
XhoI CTCGAG 1 cut(s) 476
XmiI GTMKAC 1 cut(s) 867
XmnI GAANNNNTTC 5 cut(s) 1605, 1746, 2475, 2522, 2797
ZrmI AGTACT 1 cut(s) 2645
Zsp2I ATGCAT 4 cut(s) 373, 876, 2558, 2770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.