RchiOBHm_Chr1g0375951

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000067
Physical Location & Seq
Forward (+)
63233779 .. 63241249
7471 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59963

Sequence Viewer

Length: 4050 bp
ATGGCCGCTTCTTCTTCTTCTTCGTCTTCTGCAGCTGTCGGTCGTTGGAACTACGATGTCTTCATCAGTTTTAGAGGCGATGACACTCGCAAGATCTTTGTCGGCCATCTCTACAAAGCTCTCCATCAGAAAGCAATCAACACCTTCATCGATTCCGACGAGCTTAGAAACGGTAACAACCCTTCAGAGCTTCTGGAAGCCATCGCCGACTCGAGGCTTTCCGTTGTGGTGTTCTCTAAACACTATGCTTCTTCCGAGTGGTGCCTCAGAGAGCTGGTCAAGATCGTCGAGTGCATGAAAACGATGAAGCAGATTGTGGTGCCCGTTTTCTACGAACTTGATAGTTCCGATATTCGGAAACTCAAGGCGAGTTTTGAGCAAGCTTTTGCTGAACATTTGCATGATTCTAATGCCGATGTGGAAGAGGTGCAGAGGTGGAAGTCTGCTCTAGTTGAGGCCTCCAATTTATCTGGCTGGGATTCAAGAAATTATGAGGATGACGCCAAGCTTATTGAGAAAATAGTCGGTGATATTTCTAAAAGATTGATCCGCGTCTCATCCGGTATAGACAATGTTTTGGTTGGAATGGATAAGCACATTGCAGAAATAGATGTACTATTATGTCTGGGGGTGGATGATGTTCGTGGTATTGGAATATTCGGAATGCCTGGTATAGGTAAAACAACCATTGCTCGAGCTGTTTATGATGAAATCACTTGTCAGTTTGAACACTATTGCTTTCTTGAAAATGTCAAAGATGGTTTCAAGAATAAAGGTGCTGTACATATGCAGGAAGAACTTCTATCTAGAATCTTGAAGAAAAAGGTGTGTACCTTAGGTAATTTGAATACAGGCTTCAAGATGGTTATGGAAAGGCTGAATAAGAAAAGAGTTTTGCTTGTTCTTGATGATGTGGAAAATTTTTCCCAAATTGAAGCCTTACTCGGAAAGCAATGTTCATTTGGTGGTGGAAGTAGAATCATTATAACAACTAGAGATATGCAATCACTAAGTGGAGTGCAGGAGAGATATAATCCCAAATTTTTAAGTGATGATGAAGATCTTGAGCTTTTTATGCAGTATGCGTTCAGAACACACCAACCCACAGGAGATTACGATTCTCTCTCAAGGCTTGCCATAGAATATGCTCAAGGTCTGCCTTTAGGACTTAAAGTTTTGGGAGCTTTTCTTGATAGCAAAAGTGCATGTGAATGGGAAGATGAGCTAGAAAGAATAAAGAAAATCCCACACATAGAAATTCAGGGTCTGCTTAGAACAAGCTTTGATGGACTCGATCCTTTACAAAAGGATATATTTCTAGATATTGCATGCTTCTTCAGAGGAATGAACAAAGGATATGTAACAAAAGTTCTGGAAAGTTGTGGTTTTTACCCCCATAGTGGATTACGAGTACTACTCGATAGAGCTCTCATCACGATCTCAGATGACAATAGGCTCGAAATGCATGATTTACTACAAGAAATAGGTTGGGAAATCGTCCGACGACAATCAATTAAAGAACCTGGGAAGCGCAGTAGGTTGTGGGTTTATGAAGATGTCGATCATGTGTTCACTCAAAATACGGCTACAGATGCAGTTGAGGGCATAATGCTTGACTTGTCAAGATCAAAAGAGGTATACTTAGCTTCCGAAGCTTTTGTTAAAATGACGAGGCTGAGACTGCTAAGAATTATCAGTAATAACCATTCACGTCAGGATTTATGTCATCCAAGTGATGACACTCAACAACATCTGAGTGGCGACTTCAAGTTTCTTTCTAATGAGTTGAGGATTCTCCACTGGCATGGATTCCCCTTAAAGTCTTTGCCATCCAATTTTATCCCGAAGAATCTTGTTGAACTTGACATGCGTTCTAGTCACATTGAACAACTGTGGGAAGGAATCAAGCCTCTGAAAAAGTTGACAATTATTAATCTAAGTCACTCTCAATACCTTAAGAAAATACCTGACTTCACTGAGGCGATGAATATGAAGAAACTAATTCTTGATGGTTGTTCAAGTTTATTGGAGGTTCATCCGTCCATTTCAGCTCTTAAAAACCTTGTTGTCCTGAGTCTAAAAGGGTGCAAAGCAGTTCAGAGTCTTCCGGGTAGCATTTACATGAAATCTCTTAAGACCCTTGATCTTTCTGGTTGCTCAAATCTTAAGATGTTTCCAGAGATCTCAGGAATTATGGAGGACCTATCAGAGCTTTATTTAAATGAGACTGCAATTGAAAAATTACCGTCATCGATTGAACAGCTTCAGGGGCTTGTGTTATTGAATATGGCAGACTGCAGAAGCCTTGTCTGTCTTCCAGACAACATCTGTAATTTGGCATACCTTACAAATCTCACTCTCTCTGGGTGCTCAAAACTTTATCGTTTGCCTGAGAACTTGGGGAATTTAGAATCCTTGATGATCCTTGAAATAGAAAGAAGTGGTATAAAAGAACTCCCTTTTTCGATCTTACGTGTGAATCAGCTTAAAAGATTATCATGTGATGGATGTAAAGAAATGATGGTGCCCCTTTCATCATGGTCATCATCAATCAAAGGATATTATACTTACTCTGGTATGCTACATCTTGATTTAAATGACTGCAATCTGATGGTATTATCAGATGGAATTGCTCATTTGTCCTCATTAAGAACATTAGAGCTGCGCAGAAACAACTTGGAGAGCTTACCTGCAGCAATGAATCGACTTCGTCGTTTATCACATCTTGAATTAGAAGCATGCAAGAGATTGAAATCAATACCTGAGCTTTCATCAAGTATTAGTTACATAGATGCACATGATTGCACAGCTTTGGAAACGGTTTCAACACCAAAACCTCGCTCTTCTACAAATCTTTGCTTCACATTTTCAAATTGCCTCCAGCTGATACAAACCAATCTATTCAGGGATATTGTGGAAACTTATTCCCATTATCAGGGTAGCTATCTACGACCTCTCTCCTTTAGTATGTCACTTCCTGGAAGTGAAATTCCTCATTGGTTCAACCATCAGTGTAAGGGATTCTTAGTGAATGTGCAGCTACCACAGAATTGGTTTGATGATAAGTTTTTGGGATTTGCTATATGCGGTGTTAGTAACTTCAAGGGTGCCCATAATGATGCATCCGATCTATCTGCTGTTTGTTTTTGTACCTTCAAAGGAAATCATGGAGAGTACAAGTTCAGTTTTCATTTGCTTGATTGGGGTTTCAGAACAAACAGATTCCTCCAGTCAGATCACATGTTCCTGGGATATGTGCCATGGTCGCAATATCGCTTCAGTGATGGAGGAAAGCCGGTCAATGAACGCTACTACACTGAAGCCACATTTGAGATTTTGGTAGAAAATGGAGTCTATGCACATGATGATTACAGGACAGTAATACGACGGCATTGCATCACAAGTTGCGGAGTGCGTTTGTTTCATGGTAATTACATGGAACCGCAAAATCTAAGCATCACTCAGCCTAATTCTCATCATGATAGCAGTGAAATCCTATTGGACACTGCGAGGAAGAGAAAGAGAGAAATAGCAGGGGTGAAAATGCAGTGGAAGTGGCCTCCCGCAGAAGAGGTGTTCACTGAGCAAGAGCAAGAAGGATCCCTATCAAGGGAAGGGTCCCTGGTGGGGGAAGGGTCCTCCGCGGGCAAGAGGTCCTCGGCGGGGGAAGGGGTTCTTTCTATCCTTTCTTCAGTAGCAACAGTAGAAAGTGGTGCCAATGAACGTGATGGTCCAACTATCCCTAATCGTACTAACAGAAGAAGGCGCACAAATTTGCGAAATTCGGCAACGTGGATACGGGATACAAATCAACTTATTGATGACGGCCATGCTTGGAGAAAGTATGGCCAAAAGGAGATACTTGGATCCAGATATCCCAGAAACTATTACAGATGCACTTATCGAATGGATCAAGGCTGTATGGCCACAAAGCACGTGCAACGCATCAGTGACGAGCCAGTATTATATAGAACTACTTACAGAGGAAATCACACATGCAGACCCGTCTCCAGCACACAGGACATAGAAGAGTTCTATTTGGCATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1349

Amino Acids

153.3

Weight (kDa)

6.55

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 17 - 183 1.8e-51 TIR domain
TIR_2 PF13676 20 - 117 2e-14 TIR domain
NB-ARC PF00931 212 - 365 5.7e-17 NB-ARC domain
WHD_ROQ1 PF23282 435 - 504 3.8e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 617 - 636 5.9e-07 Leucine Rich Repeat
LRR_14 PF23598 677 - 789 8.3e-09 Leucine-rich repeat region
LRR_13 PF23286 708 - 793 3.9e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 775 - 840 5.9e-09 Leucine-rich repeat region
LRR_14 PF23598 862 - 923 6.8e-07 Leucine-rich repeat region
LRR_8 PF13855 862 - 914 2.9e-06 Leucine rich repeat
C-JID PF20160 992 - 1143 5.7e-19 C-JID domain
WRKY PF03106 1274 - 1333 7e-24 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 986
Acc16I TGCGCA 1 cut(s) 2666
Acc36I ACCTGC 1 cut(s) 2698
AccB1I GGYRCC 5 cut(s) 261, 319, 2524, 3107, 3713
AccI GTMKAC 1 cut(s) 1638
AccII CGCG 2 cut(s) 552, 3644
AciI CCGC 9 cut(s) 6, 550, 3087, 3408, 3443, 3564, 3642, 3644, 3662
AclWI GGATC 8 cut(s) 541, 1289, 2416, 3594, 3607, 3861, 3874, 3918
AcoI YGGCCR 5 cut(s) 3, 103, 3826, 3847, 3924
AcsI RAATTY 7 cut(s) 919, 1040, 1257, 2404, 2988, 3772, 3781
AcuI CTGAAG 6 cut(s) 168, 1321, 2249, 3262, 3339, 3675
AcvI CACGTG 1 cut(s) 3937
AcyI GRCGYC 1 cut(s) 501
AdeI CACNNNGTG 1 cut(s) 1013
AfaI GTAC 7 cut(s) 615, 783, 832, 1413, 3151, 3176, 3751
AflII CTTAAG 3 cut(s) 1955, 2132, 2165
AflIII ACRYGT 2 cut(s) 2473, 3240
AjiI CACGTC 1 cut(s) 1712
AjnI CCWGG 5 cut(s) 667, 1522, 2977, 3246, 3621
Alw21I GWGCWC 2 cut(s) 1429, 2372
Alw26I GTCTC 4 cut(s) 559, 1672, 2219, 4012
AlwI GGATC 8 cut(s) 541, 1289, 2416, 3594, 3607, 3861, 3874, 3918
AlwNI CAGNNNCTG 1 cut(s) 1267
Ama87I CYCGRG 2 cut(s) 211, 693
AoxI GGCC 7 cut(s) 3, 103, 456, 3557, 3826, 3847, 3924
ApeKI GCWGC 4 cut(s) 32, 2662, 2693, 3037
ApoI RAATTY 7 cut(s) 919, 1040, 1257, 2404, 2988, 3772, 3781
AseI ATTAAT 1 cut(s) 1932
Asp700I GAANNNNTTC 3 cut(s) 798, 2262, 3672
AspLEI GCGC 3 cut(s) 1533, 2667, 3768
AspS9I GGNCC 5 cut(s) 2200, 3618, 3636, 3654, 3731
AsuC2I CCSGG 1 cut(s) 2109
AsuHPI GGTGA 2 cut(s) 539, 3550
AvaI CYCGRG 2 cut(s) 211, 693
AvaII GGWCC 5 cut(s) 2200, 3618, 3636, 3654, 3731
AxyI CCTNAGG 1 cut(s) 835
BaeGI GKGCMC 3 cut(s) 324, 2529, 3112
BalI TGGCCA 2 cut(s) 3849, 3926
BamHI GGATCC 2 cut(s) 3599, 3866
BanI GGYRCC 5 cut(s) 261, 319, 2524, 3107, 3713
BanII GRGCYC 1 cut(s) 1429
BarI GAAGNNNNNNTAC 2 cut(s) 166, 198
BbrPI CACGTG 1 cut(s) 3937
BbsI GAAGAC 4 cut(s) 18, 52, 2096, 2306
Bbv12I GWGCWC 2 cut(s) 1429, 2372
BbvI GCAGC 4 cut(s) 44, 2649, 2705, 3049
BceAI ACGGC 3 cut(s) 1599, 3404, 3841
BcgI CGANNNNNNTGC 4 cut(s) 3116, 3150, 3375, 3409
BciT130I CCWGG 5 cut(s) 669, 1524, 2979, 3248, 3623
BciVI GTATCC 2 cut(s) 3789, 3796
BcnI CCSGG 1 cut(s) 2109
BcoDI GTCTC 4 cut(s) 559, 1672, 2219, 4012
BfaI CTAG 6 cut(s) 449, 807, 993, 1226, 1319, 1875
BfmI CTRYAG 4 cut(s) 30, 1587, 2296, 2691
BfrI CTTAAG 3 cut(s) 1955, 2132, 2165
BfuAI ACCTGC 1 cut(s) 2698
BfuI GTATCC 2 cut(s) 3789, 3796
BglII AGATCT 3 cut(s) 93, 1060, 2181
BisI GCNGC 5 cut(s) 6, 33, 2663, 2694, 3038
BlsI GCNGC 5 cut(s) 7, 34, 2664, 2695, 3039
BmcAI AGTACT 1 cut(s) 1413
Bme1390I CCNGG 6 cut(s) 669, 1524, 2109, 2979, 3248, 3623
Bme18I GGWCC 5 cut(s) 2200, 3618, 3636, 3654, 3731
BmeT110I CYCGRG 2 cut(s) 211, 693
BmgBI CACGTC 1 cut(s) 1712
BmgT120I GGNCC 5 cut(s) 2200, 3618, 3636, 3654, 3731
BmrFI CCNGG 6 cut(s) 669, 1524, 2109, 2979, 3248, 3623
BmsI GCATC 8 cut(s) 1582, 2782, 3109, 3131, 3405, 3465, 3884, 3954
BpiI GAAGAC 4 cut(s) 18, 52, 2096, 2306
BplI GAGNNNNNCTC 2 cut(s) 1401, 1433
BpmI CTGGAG 3 cut(s) 2864, 3212, 3994
Bpu10I CCTNAGC 1 cut(s) 2763
BpuEI CTTGAG 4 cut(s) 347, 1085, 1111, 1134
BpuMI CCSGG 1 cut(s) 2109
Bsa29I ATCGAT 2 cut(s) 150, 2252
BsaAI YACGTR 2 cut(s) 2474, 3937
BsaBI GATNNNNATC 5 cut(s) 1059, 1560, 2752, 3604, 3807
BsaHI GRCGYC 1 cut(s) 501
BsaJI CCNNGG 6 cut(s) 1523, 3247, 3260, 3621, 3642, 3657
BsaWI WCCGGW 1 cut(s) 560
Bse118I RCCGGY 1 cut(s) 3295
Bse1I ACTGG 3 cut(s) 1805, 3229, 3959
Bse21I CCTNAGG 1 cut(s) 835
Bse3DI GCAATG 5 cut(s) 597, 687, 959, 2703, 3391
Bse8I GATNNNNATC 5 cut(s) 1059, 1560, 2752, 3604, 3807
BseBI CCWGG 5 cut(s) 669, 1524, 2979, 3248, 3623
BseCI ATCGAT 2 cut(s) 150, 2252
BseDI CCNNGG 6 cut(s) 1523, 3247, 3260, 3621, 3642, 3657
BseGI GGATG 8 cut(s) 502, 557, 640, 1726, 1829, 2035, 2513, 3122
BseJI GATNNNNATC 5 cut(s) 1059, 1560, 2752, 3604, 3807
BseMI GCAATG 5 cut(s) 597, 687, 959, 2703, 3391
BseNI ACTGG 3 cut(s) 1805, 3229, 3959
BseSI GKGCMC 3 cut(s) 324, 2529, 3112
BseXI GCAGC 4 cut(s) 44, 2649, 2705, 3049
BseYI CCCAGC 1 cut(s) 474
BsgI GTGCAG 3 cut(s) 449, 1040, 3056
Bsh1236I CGCG 2 cut(s) 552, 3644
Bsh1285I CGRYCG 1 cut(s) 43
BshFI GGCC 7 cut(s) 5, 105, 458, 3559, 3828, 3849, 3926
BshNI GGYRCC 5 cut(s) 261, 319, 2524, 3107, 3713
BshVI ATCGAT 2 cut(s) 150, 2252
BsiEI CGRYCG 1 cut(s) 43
BsiHKAI GWGCWC 2 cut(s) 1429, 2372
BsiHKCI CYCGRG 2 cut(s) 211, 693
BsiSI CCGG 3 cut(s) 561, 2108, 3296
BslFI GGGAC 1 cut(s) 3604
BsmAI GTCTC 4 cut(s) 559, 1672, 2219, 4012
BsmBI CGTCTC 2 cut(s) 559, 4012
BsmFI GGGAC 1 cut(s) 3604
BsmI GAATGC 1 cut(s) 669
BsnI GGCC 7 cut(s) 5, 105, 458, 3559, 3828, 3849, 3926
BsoBI CYCGRG 2 cut(s) 211, 693
Bsp1286I GDGCHC 5 cut(s) 324, 1429, 2372, 2529, 3112
Bsp1407I TGTACA 1 cut(s) 781
Bsp19I CCATGG 1 cut(s) 3260
BspACI CCGC 9 cut(s) 6, 550, 3087, 3408, 3443, 3564, 3642, 3644, 3662
BspANI GGCC 7 cut(s) 5, 105, 458, 3559, 3828, 3849, 3926
BspDI ATCGAT 2 cut(s) 150, 2252
BspFNI CGCG 2 cut(s) 552, 3644
BspHI TCATGA 1 cut(s) 3478
BspMAI CTGCAG 3 cut(s) 34, 2300, 2695
BspMI ACCTGC 1 cut(s) 2698
BspPI GGATC 8 cut(s) 541, 1289, 2416, 3594, 3607, 3861, 3874, 3918
BspQI GCTCTTC 1 cut(s) 2848
BspT107I GGYRCC 5 cut(s) 261, 319, 2524, 3107, 3713
BspTI CTTAAG 3 cut(s) 1955, 2132, 2165
BsrDI GCAATG 5 cut(s) 597, 687, 959, 2703, 3391
BsrFI RCCGGY 1 cut(s) 3295
BsrGI TGTACA 1 cut(s) 781
BsrI ACTGG 3 cut(s) 1805, 3229, 3959
BssAI RCCGGY 1 cut(s) 3295
BssECI CCNNGG 6 cut(s) 1523, 3247, 3260, 3621, 3642, 3657
BssNAI GTATAC 1 cut(s) 1639
BssNI GRCGYC 1 cut(s) 501
BssT1I CCWWGG 1 cut(s) 3260
Bst1107I GTATAC 1 cut(s) 1639
Bst2UI CCWGG 5 cut(s) 669, 1524, 2979, 3248, 3623
Bst4CI ACNGT 6 cut(s) 173, 1893, 2247, 2821, 3379, 3703
Bst6I CTCTTC 5 cut(s) 417, 2848, 3509, 3564, 4023
BstACI GRCGYC 1 cut(s) 501
BstAFI CTTAAG 3 cut(s) 1955, 2132, 2165
BstAUI TGTACA 1 cut(s) 781
BstBAI YACGTR 2 cut(s) 2474, 3937
BstC8I GCNNGC 5 cut(s) 381, 1134, 1330, 2740, 3646
BstDSI CCRYGG 2 cut(s) 3260, 3642
BstENI CCTNNNNNAGG 1 cut(s) 672
BstF5I GGATG 8 cut(s) 502, 557, 640, 1726, 1829, 2035, 2513, 3122
BstFNI CGCG 2 cut(s) 552, 3644
BstHHI GCGC 3 cut(s) 1533, 2667, 3768
BstMAI GTCTC 4 cut(s) 559, 1672, 2219, 4012
BstMCI CGRYCG 1 cut(s) 43
BstMWI GCNNNNNNNGC 7 cut(s) 1075, 1462, 1592, 1652, 1681, 2269, 3265
BstNI CCWGG 5 cut(s) 669, 1524, 2979, 3248, 3623
BstNSI RCATGY 6 cut(s) 1209, 1332, 1870, 2742, 3244, 3999
BstSCI CCNGG 6 cut(s) 667, 1522, 2107, 2977, 3246, 3621
BstSFI CTRYAG 4 cut(s) 30, 1587, 2296, 2691
BstSLI GKGCMC 3 cut(s) 324, 2529, 3112
BstUI CGCG 2 cut(s) 552, 3644
BstV1I GCAGC 4 cut(s) 44, 2649, 2705, 3049
BstV2I GAAGAC 4 cut(s) 18, 52, 2096, 2306
BstX2I RGATCY 5 cut(s) 93, 1060, 2181, 3599, 3866
BstXI CCANNNNNNTGG 2 cut(s) 1805, 3051
BstYI RGATCY 5 cut(s) 93, 1060, 2181, 3599, 3866
BstZ17I GTATAC 1 cut(s) 1639
Bsu15I ATCGAT 2 cut(s) 150, 2252
Bsu36I CCTNAGG 1 cut(s) 835
BsuI GTATCC 2 cut(s) 3789, 3796
BsuRI GGCC 7 cut(s) 5, 105, 458, 3559, 3828, 3849, 3926
BsuTUI ATCGAT 2 cut(s) 150, 2252
BtgI CCRYGG 2 cut(s) 3260, 3642
BtgZI GCGATG 3 cut(s) 93, 187, 1997
BtrI CACGTC 1 cut(s) 1712
BtsCI GGATG 8 cut(s) 502, 557, 640, 1726, 1829, 2035, 2513, 3122
BtsI GCAGTG 3 cut(s) 3493, 3504, 3554
BveI ACCTGC 1 cut(s) 2698
Cac8I GCNNGC 5 cut(s) 381, 1134, 1330, 2740, 3646
CaiI CAGNNNCTG 1 cut(s) 1267
CciI TCATGA 1 cut(s) 3478
CfoI GCGC 3 cut(s) 1533, 2667, 3768
Cfr10I RCCGGY 1 cut(s) 3295
Cfr13I GGNCC 5 cut(s) 2200, 3618, 3636, 3654, 3731
Cfr42I CCGCGG 1 cut(s) 3645
ClaI ATCGAT 2 cut(s) 150, 2252
CseI GACGC 2 cut(s) 509, 541
Csp6I GTAC 7 cut(s) 614, 782, 831, 1412, 3150, 3175, 3750
CviQI GTAC 7 cut(s) 614, 782, 831, 1412, 3150, 3175, 3750
DraI TTTAAA 2 cut(s) 2220, 2595
DraIII CACNNNGTG 1 cut(s) 1013
EaeI YGGCCR 5 cut(s) 3, 103, 3826, 3847, 3924
Eam1104I CTCTTC 5 cut(s) 417, 2848, 3509, 3564, 4023
EarI CTCTTC 5 cut(s) 417, 2848, 3509, 3564, 4023
Ecl136II GAGCTC 1 cut(s) 1427
Eco130I CCWWGG 1 cut(s) 3260
Eco147I AGGCCT 1 cut(s) 458
Eco24I GRGCYC 1 cut(s) 1429
Eco32I GATATC 1 cut(s) 3875
Eco47I GGWCC 5 cut(s) 2200, 3618, 3636, 3654, 3731
Eco53kI GAGCTC 1 cut(s) 1427
Eco57I CTGAAG 6 cut(s) 168, 1321, 2249, 3262, 3339, 3675
Eco72I CACGTG 1 cut(s) 3937
Eco81I CCTNAGG 1 cut(s) 835
Eco88I CYCGRG 2 cut(s) 211, 693
EcoICRI GAGCTC 1 cut(s) 1427
EcoNI CCTNNNNNAGG 1 cut(s) 672
EcoO109I RGGNCCY 4 cut(s) 2200, 3618, 3636, 3654
EcoRII CCWGG 5 cut(s) 667, 1522, 2977, 3246, 3621
EcoRV GATATC 1 cut(s) 3875
EcoT14I CCWWGG 1 cut(s) 3260
EcoT22I ATGCAT 2 cut(s) 1467, 3124
EcoT38I GRGCYC 1 cut(s) 1429
ErhI CCWWGG 1 cut(s) 3260
Esp3I CGTCTC 2 cut(s) 559, 4012
FalI AAGNNNNNCTT 4 cut(s) 3008, 3040, 3660, 3692
FaqI GGGAC 1 cut(s) 3604
FauI CCCGC 3 cut(s) 3571, 3637, 3655
FauNDI CATATG 1 cut(s) 786
FblI GTMKAC 1 cut(s) 1638
Fnu4HI GCNGC 5 cut(s) 6, 33, 2663, 2694, 3038
FokI GGATG 8 cut(s) 509, 544, 647, 1713, 1816, 2022, 2520, 3109
FriOI GRGCYC 1 cut(s) 1429
Fsp4HI GCNGC 5 cut(s) 6, 33, 2663, 2694, 3038
FspBI CTAG 6 cut(s) 449, 807, 993, 1226, 1319, 1875
FspI TGCGCA 1 cut(s) 2666
GlaI GCGC 3 cut(s) 1532, 2666, 3767
GluI GCNGC 5 cut(s) 6, 33, 2663, 2694, 3038
GsaI CCCAGC 1 cut(s) 478
GsuI CTGGAG 3 cut(s) 2864, 3212, 3994
HaeIII GGCC 7 cut(s) 5, 105, 458, 3559, 3828, 3849, 3926
HapII CCGG 3 cut(s) 561, 2108, 3296
HgaI GACGC 2 cut(s) 509, 541
HhaI GCGC 3 cut(s) 1533, 2667, 3768
Hin1I GRCGYC 1 cut(s) 501
Hin6I GCGC 3 cut(s) 1531, 2665, 3766
HinP1I GCGC 3 cut(s) 1531, 2665, 3766
HincII GTYRAC 1 cut(s) 1923
HindII GTYRAC 1 cut(s) 1923
HindIII AAGCTT 4 cut(s) 381, 506, 1279, 1653
HpaII CCGG 3 cut(s) 561, 2108, 3296
HphI GGTGA 2 cut(s) 539, 3550
Hpy166II GTNNAC 5 cut(s) 831, 1572, 1639, 1923, 3579
Hpy8I GTNNAC 5 cut(s) 831, 1572, 1639, 1923, 3579
Hpy99I CGWCG 5 cut(s) 161, 290, 1506, 2715, 3390
HpyAV CCTTC 9 cut(s) 154, 192, 1892, 3163, 3590, 3608, 3626, 3662, 3756
HpyCH4III ACNGT 6 cut(s) 173, 1893, 2247, 2821, 3379, 3703
HpyCH4IV ACGT 5 cut(s) 1711, 2473, 3724, 3791, 3936
HpyF10VI GCNNNNNNNGC 7 cut(s) 1075, 1462, 1592, 1652, 1681, 2269, 3265
HpySE526I ACGT 5 cut(s) 1711, 2473, 3724, 3791, 3936
Hsp92I GRCGYC 1 cut(s) 501
HspAI GCGC 3 cut(s) 1531, 2665, 3766
KflI GGGWCCC 1 cut(s) 3618
KspI CCGCGG 1 cut(s) 3645
LguI GCTCTTC 1 cut(s) 2848
LmnI GCTCC 1 cut(s) 1181
Lsp1109I GCAGC 4 cut(s) 44, 2649, 2705, 3049
LweI GCATC 8 cut(s) 1582, 2782, 3109, 3131, 3405, 3465, 3884, 3954
MaeI CTAG 6 cut(s) 449, 807, 993, 1226, 1319, 1875
MaeII ACGT 5 cut(s) 1711, 2473, 3724, 3791, 3936
MaeIII GTNAC 8 cut(s) 173, 1360, 1877, 1940, 2783, 2970, 3095, 3950
MfeI CAATTG 1 cut(s) 2232
MflI RGATCY 5 cut(s) 93, 1060, 2181, 3599, 3866
MhlI GDGCHC 5 cut(s) 324, 1429, 2372, 2529, 3112
MlsI TGGCCA 2 cut(s) 3849, 3926
MluNI TGGCCA 2 cut(s) 3849, 3926
MlyI GAGTC 5 cut(s) 203, 1284, 2083, 2110, 3360
MmeI TCCRAC 5 cut(s) 26, 180, 562, 1525, 3758
Mox20I TGGCCA 2 cut(s) 3849, 3926
Mph1103I ATGCAT 2 cut(s) 1467, 3124
MroXI GAANNNNTTC 3 cut(s) 798, 2262, 3672
MscI TGGCCA 2 cut(s) 3849, 3926
MslI CAYNNNNRTG 7 cut(s) 399, 1210, 1731, 1755, 1803, 2120, 3117
Msp20I TGGCCA 2 cut(s) 3849, 3926
MspA1I CMGCKG 3 cut(s) 35, 2884, 3644
MspCI CTTAAG 3 cut(s) 1955, 2132, 2165
MspI CCGG 3 cut(s) 561, 2108, 3296
MspR9I CCNGG 6 cut(s) 669, 1524, 2109, 2979, 3248, 3623
MunI CAATTG 1 cut(s) 2232
Mva1269I GAATGC 1 cut(s) 669
MvaI CCWGG 5 cut(s) 669, 1524, 2979, 3248, 3623
MvnI CGCG 2 cut(s) 552, 3644
MwoI GCNNNNNNNGC 7 cut(s) 1075, 1462, 1592, 1652, 1681, 2269, 3265
NciI CCSGG 1 cut(s) 2109
NcoI CCATGG 1 cut(s) 3260
NdeI CATATG 1 cut(s) 786
NmeAIII GCCGAG 1 cut(s) 3638
NmuCI GTSAC 4 cut(s) 1877, 1940, 2970, 3950
NsbI TGCGCA 1 cut(s) 2666
NsiI ATGCAT 2 cut(s) 1467, 3124
NspI RCATGY 6 cut(s) 1209, 1332, 1870, 2742, 3244, 3999
PaeI GCATGC 2 cut(s) 1332, 2742
PaeR7I CTCGAG 2 cut(s) 211, 693
PagI TCATGA 1 cut(s) 3478
PceI AGGCCT 1 cut(s) 458
PciI ACATGT 1 cut(s) 3240
PciSI GCTCTTC 1 cut(s) 2848
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 2710
PctI GAATGC 1 cut(s) 669
PdmI GAANNNNTTC 3 cut(s) 798, 2262, 3672
PflFI GACNNNGTC 1 cut(s) 2709
PfoI TCCNGGA 1 cut(s) 2977
PkrI GCNGC 5 cut(s) 7, 34, 2664, 2695, 3039
PleI GAGTC 5 cut(s) 203, 1284, 2082, 2109, 3359
PmaCI CACGTG 1 cut(s) 3937
PmlI CACGTG 1 cut(s) 3937
PpsI GAGTC 5 cut(s) 203, 1284, 2082, 2109, 3359
Ppu21I YACGTR 2 cut(s) 2474, 3937
PpuMI RGGWCCY 4 cut(s) 2200, 3618, 3636, 3654
PscI ACATGT 1 cut(s) 3240
PshBI ATTAAT 1 cut(s) 1932
PsiI TTATAA 1 cut(s) 986
Psp124BI GAGCTC 1 cut(s) 1429
Psp5II RGGWCCY 4 cut(s) 2200, 3618, 3636, 3654
Psp6I CCWGG 5 cut(s) 667, 1522, 2977, 3246, 3621
PspCI CACGTG 1 cut(s) 3937
PspFI CCCAGC 1 cut(s) 474
PspGI CCWGG 5 cut(s) 667, 1522, 2977, 3246, 3621
PspPI GGNCC 5 cut(s) 2200, 3618, 3636, 3654, 3731
PspPPI RGGWCCY 4 cut(s) 2200, 3618, 3636, 3654
PspXI VCTCGAGB 2 cut(s) 211, 693
PsrI GAACNNNNNNTAC 2 cut(s) 1353, 1385
PstI CTGCAG 3 cut(s) 34, 2300, 2695
PstNI CAGNNNCTG 1 cut(s) 1267
PsuI RGATCY 5 cut(s) 93, 1060, 2181, 3599, 3866
PsyI GACNNNGTC 1 cut(s) 2709
PvuII CAGCTG 2 cut(s) 35, 2884
RsaI GTAC 7 cut(s) 615, 783, 832, 1413, 3151, 3176, 3751
RsaNI GTAC 7 cut(s) 614, 782, 831, 1412, 3150, 3175, 3750
RseI CAYNNNNRTG 7 cut(s) 399, 1210, 1731, 1755, 1803, 2120, 3117
SacI GAGCTC 1 cut(s) 1429
SacII CCGCGG 1 cut(s) 3645
SapI GCTCTTC 1 cut(s) 2848
SatI GCNGC 5 cut(s) 6, 33, 2663, 2694, 3038
Sau96I GGNCC 5 cut(s) 2200, 3618, 3636, 3654, 3731
ScaI AGTACT 1 cut(s) 1413
SchI GAGTC 5 cut(s) 203, 1284, 2083, 2110, 3360
ScrFI CCNGG 6 cut(s) 669, 1524, 2109, 2979, 3248, 3623
SduI GDGCHC 5 cut(s) 324, 1429, 2372, 2529, 3112
SfaNI GCATC 8 cut(s) 1582, 2782, 3109, 3131, 3405, 3465, 3884, 3954
SfcI CTRYAG 4 cut(s) 30, 1587, 2296, 2691
Sfr274I CTCGAG 2 cut(s) 211, 693
Sfr303I CCGCGG 1 cut(s) 3645
SgrBI CCGCGG 1 cut(s) 3645
SinI GGWCC 5 cut(s) 2200, 3618, 3636, 3654, 3731
SlaI CTCGAG 2 cut(s) 211, 693
SmiI ATTTAAAT 2 cut(s) 2220, 2595
SmiMI CAYNNNNRTG 7 cut(s) 399, 1210, 1731, 1755, 1803, 2120, 3117
SmlI CTYRAG 9 cut(s) 211, 362, 693, 1064, 1126, 1149, 1955, 2132, 2165
SmoI CTYRAG 9 cut(s) 211, 362, 693, 1064, 1126, 1149, 1955, 2132, 2165
SphI GCATGC 2 cut(s) 1332, 2742
SseBI AGGCCT 1 cut(s) 458
SsiI CCGC 9 cut(s) 6, 550, 3087, 3408, 3443, 3564, 3642, 3644, 3662
SspI AATATT 1 cut(s) 657
SspMI CTAG 6 cut(s) 449, 807, 993, 1226, 1319, 1875
SstI GAGCTC 1 cut(s) 1429
StuI AGGCCT 1 cut(s) 458
StyD4I CCNGG 6 cut(s) 667, 1522, 2107, 2977, 3246, 3621
StyI CCWWGG 1 cut(s) 3260
SwaI ATTTAAAT 2 cut(s) 2220, 2595
TaaI ACNGT 6 cut(s) 173, 1893, 2247, 2821, 3379, 3703
TaiI ACGT 5 cut(s) 1714, 2476, 3727, 3794, 3939
TaqII GACCGA 1 cut(s) 29
TatI WGTACW 4 cut(s) 613, 781, 1411, 3174
TauI GCSGC 1 cut(s) 8
TseFI GTSAC 4 cut(s) 1877, 1940, 2970, 3950
TseI GCWGC 4 cut(s) 32, 2662, 2693, 3037
Tsp45I GTSAC 4 cut(s) 1877, 1940, 2970, 3950
TspGWI ACGGA 2 cut(s) 211, 2028
Tth111I GACNNNGTC 1 cut(s) 2709
Vha464I CTTAAG 3 cut(s) 1955, 2132, 2165
VpaK11BI GGWCC 5 cut(s) 2200, 3618, 3636, 3654, 3731
VspI ATTAAT 1 cut(s) 1932
XagI CCTNNNNNAGG 1 cut(s) 672
XapI RAATTY 7 cut(s) 919, 1040, 1257, 2404, 2988, 3772, 3781
XbaI TCTAGA 2 cut(s) 806, 1318
XceI RCATGY 6 cut(s) 1209, 1332, 1870, 2742, 3244, 3999
XhoI CTCGAG 2 cut(s) 211, 693
XmiI GTMKAC 1 cut(s) 1638
XmnI GAANNNNTTC 3 cut(s) 798, 2262, 3672
XspI CTAG 6 cut(s) 449, 807, 993, 1226, 1319, 1875
ZrmI AGTACT 1 cut(s) 1413
Zsp2I ATGCAT 2 cut(s) 1467, 3124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.