MD04G1022500.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000067
Physical Location & Seq
Reverse (-)
2635102 .. 2641369
6268 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1022500.v1.1.491

Sequence Viewer

Length: 3255 bp
ATGGACTCGGACGGCCAGACTGTGGTGCCCGTCTTCTACGACGTCGATCCGTCTCACGTCCGCAAGCGCAAGAGGAGTTTTGCGGAGGCGTTTGCGAGACACGAACTCGACTCCGCGGCTGAGATGGCGGAGGTGCAGAGCTGGAAGTCAGCTCTTACAAGAGCCTGCAATTTATCCGGCTGGGATTCGCAAAATTACAAGGATGATGCAAGGCTTGTTGAAGAAATTGTACAAGATATCTTTGATAAATTGATCCACATGTCGTCAAGTAAAGGCGATGGCTTGGTTGGAATGGATTCCCACCTAGATGAAATGAATTTGCGATTATGTCTTGGCACGGAGGATGTTCGCTTTGTTGGAATATGGGGTATGGGTGGAATAGGCAAAACAACCATTGCCAGAGCTGTTTATGAGGAAATCGCTTGTCAATTTGAAGCTTACTGCTTTCTTGAAAATGTCAAGGAAGGTTTCTCCAGGCATGGTGCAATACATATGCAGGAAGAGCTTTTATCCAGAATATTGAAGGAAAAGGTGCGAAGTTTAGGAACTTTGGATAAAGGTTCCAAGATGATAAGGAAAGGACTAGCTAAGAAAAAAGTTTTCCTTGTTCTTGATGACGTGGACAATATAGACCAAATTGAAGGCTTACTGGGAAAGCAACGTTCATTTGGCTGCGGAAGTAGAATTATTATAACAACACGAGATGTGCAATTACTAAACGGAGTTGATGCAATATATTGGCCCAAGTTTTCAAGTGACAAGGAAGCTCGTGACCTCTTTATGCAATATGCCTTCAGAACAAACCAACCCACAAGAGACCACAAACATCTCACATGCCATGCCATAGAATATGCTCGAGGTTTGCCTTTAGCGCTCAAAGTCTTGGGAGCTTTTCTTGATAATAAAAGTGTACGCGAGTGGGAAGATGTGTTAGAGAAGATAAGGAAAACCCCACACAGGGGAATTCAGGATGTGCTTAGAACGAGTTTTGATGGGCTGGACGATTCGGAGGACATTTTTTTGGACATTGCTTGTTTCTTTAAAGGAATGAAAAATGATTATGCAATAAATATCCTGGACGGTTGTGGCTTCTATCCTAATAGTGGATTAAGAGTTCTAATTGATAGATCTCTCATAACAATATCAGATGATGGCACAATCGAGATGCATGATTTACTAGAAGAAATGGGTTGGGAAATTGTCCGCCAAGAATCTATTAAAGAGCCCGGGAGACGCAGTAGGCTGTGGAGTTATGAAGATGTTCATCCCGTGTTAACTCAAAATATAGCTACAAAAGCTATTGAGAGCATAGTCCTGGACTTGTCAAACAAATCAGAAGAAGTATACTTAAATGCTGAAGCTTTTGCCGGAATGACGAGGCTAAGACTGCTCCGTATCAAATATCATTACCCCTTTAGTGACCATGATTTCATACAACACCTGAGTGGAGACTTAAAGTATCTTTCTCATGAGTTGAGGTGTCTCTTTTGGCATGGCTGCCCCCTAAAGTCTTTGCCATCCAATTTTCACCCCAACAACCTAGTTGACCTTGACATGCAATATAGTAACATCCAACAACTTTGGGAAGGAACCAAGCATTTGGAAAGGTTGAAGTTCATCAATTTAAACCACTCTCATTACCTTGAAAGAACCCCCTACTTAACCGAGGTGAAAAATCTGGAGGAATTATTCCTTGAAGATTGTAACAGTTTATTTGAGGTTCATCCATCCATTTCATCTCTTCAAAATCTTCTTGTTTTGAGTCTAAGAGGGTGCAAAGAACTGAGGAAATTTCCAAGCAGCATTTGTATGAAATCTCTCAAAAGCCTTATTCTTTCCGGCTGCTCTAGTTTTGAGAAGTTTCCAGAGATCTCAGACGTCATGAAGGAACTTTCAAACCTTCATTTAGATGGGATTGCAATTAAAGAACTGCCCTCGTCAATTAAAAATCTTACAGGGCTTGTTACTTTGAACTTAAAAGATTGCATAGAGCTTCAAAGTCTTCCAAGCAGCATTTATTTGAGATCTCTCAAATATCTTAATCTTTCTGGCTGCTCAAATTTGCTAAAGTTCCCAGAGATTTCAGAAATTTCAGAAGTTATGGGAGAACTCCAGTCGCTTTGTTTGGATGATACTGCAATTGAAGAACTGCCATCGTCAATAAGTAATCTGACGGGGCTTTGTTATTTGAGCCTTAAAGGCTGCTTGAAGCTTAAAAGTCTGCCTTGCAGTATCTGTCAACTCAAGTCCCTGAAAGATTTTAATCTTGCTGGCTGTTCAAATCTTGAGAAGTTTCTAGAGAGATTTCAGAAGTTATGGGAGAACTCAAATTGCTTTATTTGGATGGGACTGCAATTGAAGAACTGCCATCAGATTTCAGAAGTTATGGGAGAACTCCAATTGCTTTGTTTGGATGGTACTGCAATTGAAGAACTACCATCGTCGATAAGTAATCTTACGGGGCTTTGTGATTTTAGCCTTAAAGGCTGCTTGAAGCTTAAAAGTTTGCCTTGCAGTATCTGTCAACTCAAGTCTCTGGAAGATTTTAATCTTGCTGGCTGCTCAAATCTTGAGAAGTTTCCAGAAATTTCAGAAGTTATGGGAGAACTCAAATGGCTTTATTTGGATGATACTGCAATTGAAGAACTACCATCGTCGATAAGTAATCTTACGAGGCTTTGTGATTTGAGCCTTGGAGGTTGCTTGAAGCTTAAAAGTCTGCCACTGATCTATCAACTCAAGTCCCTGGAAGATTTTAATCTTGCTGGGAGTTCAAATCTTGAGAAGTTTCCAGAAATTTCAGAAGTTATGGGAGAACTCAAATGGCTTTATTTGGATGATACTGCAATTGAAGAACTACCATCGTCGATAAGTAATCTTACGAGGCTTTGTGATTTGAGCCTTGGAGGCTGCTTGAAGCTTAAAAGTCTGCCACTGATCTATCAACTCAAGTCCCTGGAAGATTTTAATCTTGCTGGGAGTTCAAATCTTGAGAAGTTTCCAGAGATTTCAGAAGTTATGGGAGAACTCAAATGGCTTTGTTTGGATGAGACTACAATTGAAAAACTGCCATCGTCGATAAAACTGCCATCGTCGATAAGTAATCTTACGGGGCTTTGTTATTTGAGCCTTGAAGGCTGCTTGAAGCTTAAAAGTCTGCCATGCAGTATCTGTCAACTCAAGTTTTTGAAAGATTTTAAACTTGCTCGCTGCTCAAATCTTGAGAAGTTTCTAGAGATTTCAGAAGTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1085

Amino Acids

122.56

Weight (kDa)

5.39

Isoelectric Point (pI)

46.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 3 - 97 4e-17 TIR domain
NB-ARC PF00931 102 - 268 2.1e-16 NB-ARC domain
WHD_ROQ1 PF23282 337 - 405 4e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 513 - 532 6.7e-08 Leucine Rich Repeat
LRR_13 PF23286 605 - 674 3.4e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 673 - 769 9.4e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 674 - 772 3e-08 Leucine-rich repeat region
LRR_13 PF23286 796 - 858 5.6e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 843 - 927 3.7e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 913 - 995 1.6e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 983 - 1076 2e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000067)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g01690 FvH4_6g01690 FvH4_7g11151 FvH4_7g11160 FvH4_7g11570 FvH4_7g17700 FvH4_7g26071 FvH4_7g28510 FvH4_7g28520 FvH4_7g28530 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28540 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550 FvH4_7g28550
malus_domestica MD00G1020300.v1.1 MD00G1020400.v1.1 MD00G1020500.v1.1 MD00G1020700.v1.1 MD00G1021100.v1.1 MD00G1131100.v1.1 MD01G1188900.v1.1 MD01G1189000.v1.1 MD01G1189200.v1.1 MD01G1190100.v1.1 MD01G1190300.v1.1 MD01G1190500.v1.1 MD01G1190700.v1.1 MD01G1191400.v1.1 MD02G1260000.v1.1 MD02G1260200.v1.1 MD02G1260400.v1.1 MD03G1202400.v1.1 MD04G1022500.v1.1 MD04G1022700.v1.1 MD04G1200400.v1.1 MD07G1015300.v1.1 MD07G1015400.v1.1 MD07G1141800.v1.1 MD07G1242100.v1.1 MD07G1242500.v1.1 MD07G1243000.v1.1 MD07G1243500.v1.1 MD07G1244000.v1.1 MD07G1244600.v1.1 MD07G1245100.v1.1 MD07G1245600.v1.1 MD07G1246000.v1.1 MD07G1246200.v1.1 MD07G1260000.v1.1 MD07G1260700.v1.1 MD07G1261100.v1.1 MD08G1229600.v1.1 MD08G1229700.v1.1 MD08G1230200.v1.1 MD09G1186700.v1.1 MD09G1186800.v1.1 MD17G1208600.v1.1
pyrus_communis pycom01g20080 pycom01g20090 pycom01g20120 pycom01g20130 pycom01g20160 pycom01g20200 pycom01g20210 pycom01g20250 pycom02g22140 pycom02g22170 pycom02g22200 pycom02g22220 pycom02g22230 pycom03g15410 pycom03g15420 pycom04g01960 pycom04g01970 pycom07g21840 pycom07g21930 pycom07g21960 pycom07g22000 pycom07g23350 pycom07g23370 pycom07g23440 pycom07g23450 pycom07g23460 pycom07g23500 pycom07g23530 pycom07g23590 pycom07g23660 pycom07g23670 pycom07g23690 pycom07g23710 pycom07g23760 pycom07g23770 pycom07g23780 pycom07g23800 pycom07g23820 pycom08g19980 pycom08g19990 pycom08g20010 pycom08g20020 pycom17g04750 pycom17g21140 pycom17g21200 pycom17g21250 pycom17g21270 pycom17g21290 pycom17g21340 pycom17g21350
rosa_chinensis RchiOBHm_Chr1g0335971 RchiOBHm_Chr1g0349181 RchiOBHm_Chr1g0349211 RchiOBHm_Chr1g0349221 RchiOBHm_Chr1g0350001 RchiOBHm_Chr1g0372491 RchiOBHm_Chr1g0372501 RchiOBHm_Chr1g0372511 RchiOBHm_Chr1g0372541 RchiOBHm_Chr1g0375911 RchiOBHm_Chr1g0375921 RchiOBHm_Chr1g0375931 RchiOBHm_Chr1g0375941 RchiOBHm_Chr1g0375951 RchiOBHm_Chr3g0454321 RchiOBHm_Chr6g0266131 RchiOBHm_Chr6g0270371 RchiOBHm_Chr6g0270381 RchiOBHm_Chr6g0270391 RchiOBHm_Chr7g0232121 RchiOBHm_Chr7g0232831
rosa_laevigata RLG00000008620 RLG00000009600 RLG00000010114 RLG00000020933 RLG00000022777 RLG00000022778 RLG00000025461 RLG00000025462 RLG00000026641 RLG00000026642 RLG00000026643 RLG00000026894 RLG00000028442 RLG00000028506 RLG00000028510 RLG00000028563 RLG00000028564 RLG00000028570 RLG00000028574 RLG00000028577 RLG00000028579 RLG00000028580 RLG00000028582 RLG00000028584 RLG00000028588 RLG00000028591 RLG00000028592 RLG00000028596
rosa_multiflora Rmu_co8179714.1_g000001 Rmu_co8285207.1_g000001 Rmu_co8469031.1_g000001 Rmu_co8499245.1_g000002 Rmu_sc0000616.1_g000011 Rmu_sc0000827.1_g000022 Rmu_sc0000827.1_g000025 Rmu_sc0000827.1_g000034 Rmu_sc0000827.1_g000036 Rmu_sc0001340.1_g000001 Rmu_sc0001909.1_g000018 Rmu_sc0002863.1_g000018 Rmu_sc0002863.1_g000031 Rmu_sc0002914.1_g000028 Rmu_sc0002914.1_g000029 Rmu_sc0002914.1_g000032 Rmu_sc0003385.1_g000001 Rmu_sc0003385.1_g000008 Rmu_sc0003385.1_g000009 Rmu_sc0003385.1_g000013 Rmu_sc0003385.1_g000015 Rmu_sc0003385.1_g000025 Rmu_sc0003441.1_g000069 Rmu_sc0003674.1_g000007 Rmu_sc0003674.1_g000011 Rmu_sc0004618.1_g000001 Rmu_sc0005410.1_g000018 Rmu_sc0006415.1_g000005 Rmu_sc0006415.1_g000006 Rmu_sc0006415.1_g000014 Rmu_sc0006415.1_g000018 Rmu_sc0009606.1_g000011 Rmu_sc0011111.1_g000006 Rmu_sc0011709.1_g000003 Rmu_sc0011709.1_g000006 Rmu_sc0014584.1_g000002 Rmu_sc0015952.1_g000001 Rmu_sc0019347.1_g000001 Rmu_sc0019347.1_g000002 Rmu_sc0019347.1_g000003 Rmu_sc0023808.1_g000001 Rmu_sc0032581.1_g000001 Rmu_sc0034725.1_g000002 Rmu_sc0035038.1_g000001 Rmu_ssc0000018.1_g000005 Rmu_ssc0000018.1_g000006 Rmu_ssc0000116.1_g000002 Rmu_ssc0000150.1_g000009 Rmu_ssc0000150.1_g000011 Rmu_ssc0000472.1_g000013
rosa_roxburghii Rroxscaffold_165G00437140 Rroxscaffold_174G00435220 Rroxscaffold_3G00228770 Rroxscaffold_3G00228780 Rroxscaffold_4G00282480 Rroxscaffold_4G00282490 Rroxscaffold_4G00282510 Rroxscaffold_4G00282520 Rroxscaffold_4G00282540 Rroxscaffold_4G00284870 Rroxscaffold_4G00295380 Rroxscaffold_4G00305140 Rroxscaffold_4G00305150 Rroxscaffold_4G00305210 Rroxscaffold_4G00305760 Rroxscaffold_4G00305810 Rroxscaffold_4G00305840 Rroxscaffold_4G00305900 Rroxscaffold_4G00305920 Rroxscaffold_4G00305960 Rroxscaffold_4G00305980 Rroxscaffold_4G00305990 Rroxscaffold_4G00306020 Rroxscaffold_4G00325020 Rroxscaffold_5G00352760 Rroxscaffold_6G00425210 Rroxscaffold_7G00201950 Rroxscaffold_7G00214940
rosa_rugosa Rorug01G0048900 Rorug01G0049000 Rorug01G0201400 Rorug01G0201700 Rorug01G0202000 Rorug01G0207700 Rorug01G0207800 Rorug01G0207900 Rorug01G0286300 Rorug01G0286400 Rorug01G0286500 Rorug01G0372500 Rorug01G0372600 Rorug01G0392300 Rorug01G0392400 Rorug01G0392500 Rorug01G0392600 Rorug01G0392700 Rorug01G0392800 Rorug01G0392800 Rorug01G0392900 Rorug01G0393000 Rorug02G0654200 Rorug02G0654200 Rorug04G0027700 Rorug05G0351300 Rorug05G0351400 Rorug05G0351600 Rorug05G0406600 Rorug07G0269500 Rorug07G0276200 Rorug07G0276200
rosa_samantha Rh1AG224200 Rh1AG229900 Rh1AG297600 Rh1AG297700 Rh1AG381000 Rh1AG404200 Rh1AG404300 Rh1AG404400 Rh1BG184300 Rh1BG367600 Rh1BG367900 Rh1BG368000 Rh1CG203700 Rh1CG208900 Rh1CG279000 Rh1CG279100 Rh1CG357900 Rh1CG380500 Rh1CG380700 Rh1CG380800 Rh1CG380900 Rh1DG214000 Rh3AG058200 Rh3BG059900 Rh6BG145500 Rh6BG292400 Rh6DG129800 Rh7AG423900 Rh7AG431300 Rh7BG249300 Rh7BG404200 Rh7CG049400 Rh7CG444300 Rh7CG450000 Rh7DG420600
rosa_wichuraiana Rw0G000410 Rw0G007570 Rw0G011870 Rw1G018580 Rw1G018590 Rw1G018610 Rw1G018620 Rw1G018640 Rw1G019140 Rw1G019150 Rw1G019260 Rw1G019900 Rw1G026290 Rw1G026300 Rw1G026870 Rw1G026890 Rw1G033510 Rw1G035810 Rw1G035830 Rw1G035840 Rw1G035850 Rw1G035860 Rw3G004460 Rw5G038750 Rw7G035060 Rw7G035610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 692
AatII GACGTC 2 cut(s) 45, 1881
AccB1I GGYRCC 1 cut(s) 25
AccB7I CCANNNNNTGG 1 cut(s) 22
AccI GTMKAC 1 cut(s) 1344
AccII CGCG 2 cut(s) 116, 915
AciI CCGC 7 cut(s) 61, 83, 114, 116, 128, 675, 1204
AclI AACGTT 1 cut(s) 661
AclWI GGATC 2 cut(s) 41, 247
AcoI YGGCCR 1 cut(s) 13
AcsI RAATTY 7 cut(s) 316, 963, 1790, 2059, 2088, 2586, 2796
AcuI CTGAAG 2 cut(s) 778, 1377
AcyI GRCGYC 2 cut(s) 42, 1878
AfaI GTAC 3 cut(s) 231, 912, 2419
AfeI AGCGCT 1 cut(s) 873
AfiI CCNNNNNNNGG 5 cut(s) 22, 957, 958, 959, 1103
AflIII ACRYGT 1 cut(s) 258
AjiI CACGTC 2 cut(s) 58, 619
AjnI CCWGG 5 cut(s) 473, 1074, 1314, 2745, 2955
AleI CACNNNNGTG 1 cut(s) 1443
AloI GAACNNNNNNTCC 2 cut(s) 1098, 1130
Alw26I GTCTC 8 cut(s) 57, 91, 810, 1225, 1443, 1487, 2538, 3044
AlwI GGATC 2 cut(s) 41, 247
AlwNI CAGNNNCTG 3 cut(s) 2235, 2520, 3171
Ama87I CYCGRG 2 cut(s) 855, 1226
Aor51HI AGCGCT 1 cut(s) 873
AoxI GGCC 2 cut(s) 13, 740
ApoI RAATTY 7 cut(s) 316, 963, 1790, 2059, 2088, 2586, 2796
Asp700I GAANNNNTTC 2 cut(s) 295, 1260
AspLEI GCGC 2 cut(s) 69, 874
AspS9I GGNCC 1 cut(s) 741
AsuC2I CCSGG 2 cut(s) 1227, 1228
AsuHPI GGTGA 2 cut(s) 1520, 1681
AvaI CYCGRG 2 cut(s) 855, 1226
BaeGI GKGCMC 1 cut(s) 30
BanI GGYRCC 1 cut(s) 25
BanII GRGCYC 1 cut(s) 1227
BarI GAAGNNNNNNTAC 2 cut(s) 213, 245
BauI CACGAG 2 cut(s) 699, 768
BbsI GAAGAC 2 cut(s) 25, 1994
BceAI ACGGC 1 cut(s) 28
BcgI CGANNNNNNTGC 2 cut(s) 3067, 3101
BciT130I CCWGG 5 cut(s) 475, 1076, 1316, 2747, 2957
BcnI CCSGG 2 cut(s) 1227, 1228
BcoDI GTCTC 8 cut(s) 57, 91, 810, 1225, 1443, 1487, 2538, 3044
BfaI CTAG 7 cut(s) 305, 584, 1178, 1541, 1848, 2297, 3233
BfoI RGCGCY 1 cut(s) 875
BglI GCCNNNNNGGC 4 cut(s) 2199, 2484, 2907, 3135
BglII AGATCT 3 cut(s) 1127, 1869, 2024
Bme1390I CCNGG 7 cut(s) 475, 1076, 1227, 1228, 1316, 2747, 2957
BmeT110I CYCGRG 2 cut(s) 855, 1226
BmgBI CACGTC 2 cut(s) 58, 619
BmgT120I GGNCC 1 cut(s) 741
BmiI GGNNCC 3 cut(s) 27, 562, 1591
BmrFI CCNGG 7 cut(s) 475, 1076, 1227, 1228, 1316, 2747, 2957
BmrI ACTGGG 1 cut(s) 659
BmsI GCATC 3 cut(s) 196, 718, 1155
BmuI ACTGGG 1 cut(s) 659
BpiI GAAGAC 2 cut(s) 25, 1994
BpmI CTGGAG 3 cut(s) 457, 1700, 2096
BpuMI CCSGG 2 cut(s) 1227, 1228
BsaBI GATNNNNATC 5 cut(s) 1263, 2262, 2547, 2757, 2967
BsaHI GRCGYC 2 cut(s) 42, 1878
BsaI GGTCTC 1 cut(s) 810
BsaJI CCNNGG 7 cut(s) 114, 1226, 1665, 2692, 2745, 2902, 2955
BsaXI ACNNNNNCTCC 2 cut(s) 1222, 1252
Bsc4I CCNNNNNNNGG 5 cut(s) 22, 957, 958, 959, 1103
Bse1I ACTGG 2 cut(s) 654, 2113
Bse3DI GCAATG 2 cut(s) 393, 1026
Bse8I GATNNNNATC 5 cut(s) 1263, 2262, 2547, 2757, 2967
BseBI CCWGG 5 cut(s) 475, 1076, 1316, 2747, 2957
BseDI CCNNGG 7 cut(s) 114, 1226, 1665, 2692, 2745, 2902, 2955
BseJI GATNNNNATC 5 cut(s) 1263, 2262, 2547, 2757, 2967
BseLI CCNNNNNNNGG 5 cut(s) 22, 957, 958, 959, 1103
BseMI GCAATG 2 cut(s) 393, 1026
BseMII CTCAG 4 cut(s) 111, 1433, 1775, 1887
BseNI ACTGG 2 cut(s) 654, 2113
BseRI GAGGAG 1 cut(s) 88
BseSI GKGCMC 1 cut(s) 30
BseYI CCCAGC 3 cut(s) 180, 2765, 2975
BsgI GTGCAG 1 cut(s) 155
Bsh1236I CGCG 2 cut(s) 116, 915
BshFI GGCC 2 cut(s) 15, 742
BshNI GGYRCC 1 cut(s) 25
BsiHKCI CYCGRG 2 cut(s) 855, 1226
BsiSI CCGG 4 cut(s) 177, 1227, 1368, 1839
BslFI GGGAC 4 cut(s) 2233, 2361, 2728, 2938
BslI CCNNNNNNNGG 5 cut(s) 22, 957, 958, 959, 1103
BsmAI GTCTC 8 cut(s) 57, 91, 810, 1225, 1443, 1487, 2538, 3044
BsmBI CGTCTC 2 cut(s) 57, 1225
BsmFI GGGAC 4 cut(s) 2233, 2361, 2728, 2938
BsnI GGCC 2 cut(s) 15, 742
Bso31I GGTCTC 1 cut(s) 810
BsoBI CYCGRG 2 cut(s) 855, 1226
Bsp1286I GDGCHC 2 cut(s) 30, 1227
Bsp1407I TGTACA 1 cut(s) 229
Bsp143I GATC 7 cut(s) 46, 252, 1127, 1869, 2024, 2727, 2937
BspACI CCGC 7 cut(s) 61, 83, 114, 116, 128, 675, 1204
BspANI GGCC 2 cut(s) 15, 742
BspCNI CTCAG 4 cut(s) 112, 1434, 1776, 1886
BspFNI CGCG 2 cut(s) 116, 915
BspHI TCATGA 2 cut(s) 1468, 1881
BspLI GGNNCC 3 cut(s) 27, 562, 1591
BspPI GGATC 2 cut(s) 41, 247
BspQI GCTCTTC 1 cut(s) 495
BspT107I GGYRCC 1 cut(s) 25
BspTNI GGTCTC 1 cut(s) 810
BsrDI GCAATG 2 cut(s) 393, 1026
BsrGI TGTACA 1 cut(s) 229
BsrI ACTGG 2 cut(s) 654, 2113
BssECI CCNNGG 7 cut(s) 114, 1226, 1665, 2692, 2745, 2902, 2955
BssMI GATC 7 cut(s) 46, 252, 1127, 1869, 2024, 2727, 2937
BssNAI GTATAC 1 cut(s) 1345
BssNI GRCGYC 2 cut(s) 42, 1878
BssSI CACGAG 2 cut(s) 699, 768
BssT1I CCWWGG 2 cut(s) 2692, 2902
Bst1107I GTATAC 1 cut(s) 1345
Bst2BI CACGAG 2 cut(s) 699, 768
Bst2UI CCWGG 5 cut(s) 475, 1076, 1316, 2747, 2957
Bst4CI ACNGT 3 cut(s) 22, 1082, 1709
Bst6I CTCTTC 2 cut(s) 495, 1746
BstACI GRCGYC 2 cut(s) 42, 1878
BstAUI TGTACA 1 cut(s) 229
BstC8I GCNNGC 5 cut(s) 65, 166, 2272, 2557, 3208
BstDEI CTNAG 8 cut(s) 120, 588, 977, 1382, 1442, 1766, 1784, 1873
BstDSI CCRYGG 1 cut(s) 114
BstFNI CGCG 2 cut(s) 116, 915
BstH2I RGCGCY 1 cut(s) 875
BstHHI GCGC 2 cut(s) 69, 874
BstKTI GATC 7 cut(s) 49, 255, 1130, 1872, 2027, 2730, 2940
BstMAI GTCTC 8 cut(s) 57, 91, 810, 1225, 1443, 1487, 2538, 3044
BstMBI GATC 7 cut(s) 46, 252, 1127, 1869, 2024, 2727, 2937
BstMWI GCNNNNNNNGC 9 cut(s) 125, 502, 871, 1295, 1387, 2199, 2484, 2907, 3135
BstNI CCWGG 5 cut(s) 475, 1076, 1316, 2747, 2957
BstNSI RCATGY 3 cut(s) 262, 837, 1558
BstSCI CCNGG 7 cut(s) 473, 1074, 1225, 1226, 1314, 2745, 2955
BstSLI GKGCMC 1 cut(s) 30
BstUI CGCG 2 cut(s) 116, 915
BstV2I GAAGAC 2 cut(s) 25, 1994
BstX2I RGATCY 3 cut(s) 1127, 1869, 2024
BstXI CCANNNNNNTGG 1 cut(s) 1600
BstYI RGATCY 3 cut(s) 1127, 1869, 2024
BstZ17I GTATAC 1 cut(s) 1345
BsuRI GGCC 2 cut(s) 15, 742
BtgI CCRYGG 1 cut(s) 114
BtgZI GCGATG 1 cut(s) 291
BtrI CACGTC 2 cut(s) 58, 619
BtsIMutI CAGTG 2 cut(s) 2723, 2933
Cac8I GCNNGC 5 cut(s) 65, 166, 2272, 2557, 3208
CaiI CAGNNNCTG 3 cut(s) 2235, 2520, 3171
CciI TCATGA 2 cut(s) 1468, 1881
CfoI GCGC 2 cut(s) 69, 874
Cfr13I GGNCC 1 cut(s) 741
Cfr42I CCGCGG 1 cut(s) 117
Cfr9I CCCGGG 1 cut(s) 1226
CseI GACGC 1 cut(s) 1242
Csp6I GTAC 3 cut(s) 230, 911, 2418
CviQI GTAC 3 cut(s) 230, 911, 2418
DdeI CTNAG 8 cut(s) 120, 588, 977, 1382, 1442, 1766, 1784, 1873
DpnI GATC 7 cut(s) 48, 254, 1129, 1871, 2026, 2729, 2939
DpnII GATC 7 cut(s) 46, 252, 1127, 1869, 2024, 2727, 2937
DraI TTTAAA 3 cut(s) 1042, 1626, 3199
EaeI YGGCCR 1 cut(s) 13
Eam1104I CTCTTC 2 cut(s) 495, 1746
EarI CTCTTC 2 cut(s) 495, 1746
EciI GGCGGA 2 cut(s) 143, 1193
Eco130I CCWWGG 2 cut(s) 2692, 2902
Eco24I GRGCYC 1 cut(s) 1227
Eco31I GGTCTC 1 cut(s) 810
Eco32I GATATC 1 cut(s) 238
Eco47III AGCGCT 1 cut(s) 873
Eco57I CTGAAG 2 cut(s) 778, 1377
Eco88I CYCGRG 2 cut(s) 855, 1226
EcoRI GAATTC 1 cut(s) 963
EcoRII CCWGG 5 cut(s) 473, 1074, 1314, 2745, 2955
EcoRV GATATC 1 cut(s) 238
EcoT14I CCWWGG 2 cut(s) 2692, 2902
EcoT22I ATGCAT 1 cut(s) 1170
EcoT38I GRGCYC 1 cut(s) 1227
ErhI CCWWGG 2 cut(s) 2692, 2902
Esp3I CGTCTC 2 cut(s) 57, 1225
FalI AAGNNNNNCTT 6 cut(s) 588, 620, 2209, 2241, 2494, 2526
FaqI GGGAC 4 cut(s) 2233, 2361, 2728, 2938
FauNDI CATATG 1 cut(s) 492
FblI GTMKAC 1 cut(s) 1344
FriOI GRGCYC 1 cut(s) 1227
FspBI CTAG 7 cut(s) 305, 584, 1178, 1541, 1848, 2297, 3233
GlaI GCGC 2 cut(s) 68, 873
GsaI CCCAGC 3 cut(s) 184, 2769, 2979
GsuI CTGGAG 3 cut(s) 457, 1700, 2096
HaeII RGCGCY 1 cut(s) 875
HaeIII GGCC 2 cut(s) 15, 742
HapII CCGG 4 cut(s) 177, 1227, 1368, 1839
HgaI GACGC 1 cut(s) 1242
HhaI GCGC 2 cut(s) 69, 874
Hin1I GRCGYC 2 cut(s) 42, 1878
Hin6I GCGC 2 cut(s) 67, 872
HinP1I GCGC 2 cut(s) 67, 872
HincII GTYRAC 5 cut(s) 1275, 1546, 2240, 2525, 3176
HindII GTYRAC 5 cut(s) 1275, 1546, 2240, 2525, 3176
HindIII AAGCTT 7 cut(s) 435, 1359, 2210, 2495, 2708, 2918, 3146
HinfI GANTC 7 cut(s) 5, 110, 185, 296, 1004, 1211, 1762
HpaI GTTAAC 1 cut(s) 1275
HpaII CCGG 4 cut(s) 177, 1227, 1368, 1839
HphI GGTGA 2 cut(s) 1520, 1681
Hpy166II GTNNAC 8 cut(s) 622, 911, 1275, 1345, 1546, 2240, 2525, 3176
Hpy8I GTNNAC 8 cut(s) 622, 911, 1275, 1345, 1546, 2240, 2525, 3176
Hpy99I CGWCG 7 cut(s) 44, 47, 2446, 2659, 2869, 3079, 3097
HpyAV CCTTC 8 cut(s) 458, 517, 635, 802, 1580, 1879, 1910, 3128
HpyCH4III ACNGT 3 cut(s) 22, 1082, 1709
HpyCH4IV ACGT 5 cut(s) 42, 57, 618, 661, 1878
HpyF10VI GCNNNNNNNGC 9 cut(s) 125, 502, 871, 1295, 1387, 2199, 2484, 2907, 3135
HpyF3I CTNAG 8 cut(s) 120, 588, 977, 1382, 1442, 1766, 1784, 1873
HpySE526I ACGT 5 cut(s) 42, 57, 618, 661, 1878
Hsp92I GRCGYC 2 cut(s) 42, 1878
HspAI GCGC 2 cut(s) 67, 872
KspAI GTTAAC 1 cut(s) 1275
KspI CCGCGG 1 cut(s) 117
Kzo9I GATC 7 cut(s) 46, 252, 1127, 1869, 2024, 2727, 2937
LguI GCTCTTC 1 cut(s) 495
LmnI GCTCC 2 cut(s) 887, 1395
LweI GCATC 3 cut(s) 196, 718, 1155
MaeI CTAG 7 cut(s) 305, 584, 1178, 1541, 1848, 2297, 3233
MaeII ACGT 5 cut(s) 42, 57, 618, 661, 1878
MaeIII GTNAC 6 cut(s) 755, 770, 1418, 1565, 1703, 1963
MalI GATC 7 cut(s) 48, 254, 1129, 1871, 2026, 2729, 2939
MboI GATC 7 cut(s) 46, 252, 1127, 1869, 2024, 2727, 2937
MfeI CAATTG 7 cut(s) 2139, 2354, 2399, 2424, 2637, 2847, 3057
MflI RGATCY 3 cut(s) 1127, 1869, 2024
MhlI GDGCHC 2 cut(s) 30, 1227
MlyI GAGTC 2 cut(s) 104, 1771
MmeI TCCRAC 3 cut(s) 268, 337, 1597
Mph1103I ATGCAT 1 cut(s) 1170
MroXI GAANNNNTTC 2 cut(s) 295, 1260
MslI CAYNNNNRTG 4 cut(s) 306, 1443, 1808, 1908
MspA1I CMGCKG 1 cut(s) 116
MspI CCGG 4 cut(s) 177, 1227, 1368, 1839
MspR9I CCNGG 7 cut(s) 475, 1076, 1227, 1228, 1316, 2747, 2957
MunI CAATTG 7 cut(s) 2139, 2354, 2399, 2424, 2637, 2847, 3057
MvaI CCWGG 5 cut(s) 475, 1076, 1316, 2747, 2957
MvnI CGCG 2 cut(s) 116, 915
MwoI GCNNNNNNNGC 9 cut(s) 125, 502, 871, 1295, 1387, 2199, 2484, 2907, 3135
NciI CCSGG 2 cut(s) 1227, 1228
NdeI CATATG 1 cut(s) 492
NdeII GATC 7 cut(s) 46, 252, 1127, 1869, 2024, 2727, 2937
NlaIV GGNNCC 3 cut(s) 27, 562, 1591
NmuCI GTSAC 3 cut(s) 755, 770, 1418
NsiI ATGCAT 1 cut(s) 1170
NspI RCATGY 3 cut(s) 262, 837, 1558
OliI CACNNNNGTG 1 cut(s) 1443
PaeR7I CTCGAG 1 cut(s) 855
PagI TCATGA 2 cut(s) 1468, 1881
PciI ACATGT 1 cut(s) 258
PciSI GCTCTTC 1 cut(s) 495
PdmI GAANNNNTTC 2 cut(s) 295, 1260
PfeI GAWTC 4 cut(s) 185, 296, 1004, 1211
PflMI CCANNNNNTGG 1 cut(s) 22
PfoI TCCNGGA 2 cut(s) 1074, 1314
PleI GAGTC 2 cut(s) 104, 1770
PpsI GAGTC 2 cut(s) 104, 1770
PscI ACATGT 1 cut(s) 258
PsiI TTATAA 1 cut(s) 692
Psp1406I AACGTT 1 cut(s) 661
Psp6I CCWGG 5 cut(s) 473, 1074, 1314, 2745, 2955
PspFI CCCAGC 3 cut(s) 180, 2765, 2975
PspGI CCWGG 5 cut(s) 473, 1074, 1314, 2745, 2955
PspN4I GGNNCC 3 cut(s) 27, 562, 1591
PspPI GGNCC 1 cut(s) 741
PspXI VCTCGAGB 1 cut(s) 855
PstNI CAGNNNCTG 3 cut(s) 2235, 2520, 3171
PsuI RGATCY 3 cut(s) 1127, 1869, 2024
RsaI GTAC 3 cut(s) 231, 912, 2419
RsaNI GTAC 3 cut(s) 230, 911, 2418
RseI CAYNNNNRTG 4 cut(s) 306, 1443, 1808, 1908
SacII CCGCGG 1 cut(s) 117
SapI GCTCTTC 1 cut(s) 495
Sau3AI GATC 7 cut(s) 46, 252, 1127, 1869, 2024, 2727, 2937
Sau96I GGNCC 1 cut(s) 741
SchI GAGTC 2 cut(s) 104, 1771
ScrFI CCNGG 7 cut(s) 475, 1076, 1227, 1228, 1316, 2747, 2957
SduI GDGCHC 2 cut(s) 30, 1227
SfaNI GCATC 3 cut(s) 196, 718, 1155
Sfr274I CTCGAG 1 cut(s) 855
Sfr303I CCGCGG 1 cut(s) 117
SgrBI CCGCGG 1 cut(s) 117
SlaI CTCGAG 1 cut(s) 855
SmaI CCCGGG 1 cut(s) 1228
SmiMI CAYNNNNRTG 4 cut(s) 306, 1443, 1808, 1908
SsiI CCGC 7 cut(s) 61, 83, 114, 116, 128, 675, 1204
SspI AATATT 1 cut(s) 519
SspMI CTAG 7 cut(s) 305, 584, 1178, 1541, 1848, 2297, 3233
StyD4I CCNGG 7 cut(s) 473, 1074, 1225, 1226, 1314, 2745, 2955
StyI CCWWGG 2 cut(s) 2692, 2902
TaaI ACNGT 3 cut(s) 22, 1082, 1709
TaiI ACGT 5 cut(s) 45, 60, 621, 664, 1881
TaqI TCGA 9 cut(s) 45, 108, 856, 1161, 2444, 2657, 2867, 3077, 3095
TatI WGTACW 1 cut(s) 229
TauI GCSGC 1 cut(s) 119
TfiI GAWTC 4 cut(s) 185, 296, 1004, 1211
TscAI CASTG 2 cut(s) 2730, 2940
TseFI GTSAC 3 cut(s) 755, 770, 1418
Tsp45I GTSAC 3 cut(s) 755, 770, 1418
TspGWI ACGGA 4 cut(s) 39, 353, 735, 1382
TspMI CCCGGG 1 cut(s) 1226
TspRI CASTG 2 cut(s) 2730, 2940
Van91I CCANNNNNTGG 1 cut(s) 22
XapI RAATTY 7 cut(s) 316, 963, 1790, 2059, 2088, 2586, 2796
XbaI TCTAGA 2 cut(s) 2296, 3232
XceI RCATGY 3 cut(s) 262, 837, 1558
XhoI CTCGAG 1 cut(s) 855
XmaI CCCGGG 1 cut(s) 1226
XmiI GTMKAC 1 cut(s) 1344
XmnI GAANNNNTTC 2 cut(s) 295, 1260
XspI CTAG 7 cut(s) 305, 584, 1178, 1541, 1848, 2297, 3233
ZraI GACGTC 2 cut(s) 43, 1879
Zsp2I ATGCAT 1 cut(s) 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.